JP2008013531A - Collagen production promoter and food and beverage for beauty containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a collagen production promoter effective for improving skin aging, chapped skin, etc., and a food and beverage for beauty. <P>SOLUTION: The collagen production promoter comprises an extract of tuberous root of konjak as an active ingredient which is obtained by mixing konjak fine flour with ethanol with stirring at a normal temperature, filtering the mixture to give an extracted solution, concentrating the solution, redissolving the blackish brown wax-like concentrate in ethanol, adding cyclodextrin to the solution, mixing, concentrating, evaporating to dryness and grinding. The food and beverage for beauty comprises the collagen production promoter. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a collagen production promoter and a beauty food or drink containing the same.

  Particularly for women, skin aging (wrinkles, sagging, etc.) and rough skin are a major cosmetic issue. There are various causes of skin aging, rough skin, etc., but the underlying phenomenon is the decrease in collagen production of skin fibroblasts, decrease in hyaluronic acid synthesis, increase in collagenase activity due to ultraviolet rays, activity caused by ultraviolet rays and the environment. It is considered to be a decrease in the moisture retention function of the skin due to oxygen damage or the like, or a deterioration, degeneration or reduction of the skin components. When skin moisturizing function is affected by external factors such as the environment, internal factors such as food and luxury items, and aging, the skin becomes dry and loses elasticity, dry skin, wrinkles, etc. This leads to troubles such as atopic dermatitis.

  On the other hand, the epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrix (collagen, elastin, hyaluronic acid, etc.) that is the structural support of the skin outside these cells. These skin tissues are reborn on a daily basis through a turnover cycle, and in addition to maintaining moisture in the skin, maintaining flexibility and elasticity, they also have effects such as preventing the entry of external enemies. However, the above-mentioned external and internal factors and aging promote the decrease, degradation, and denaturation of the production of collagen, which is a major component of the extracellular matrix. As a result, the moisture of the skin decreases, and the flexibility and elasticity Sexuality is lost and causes aging phenomena such as rough skin, spots and wrinkles.

  Collagen, which is a major component of the extracellular matrix, takes a longer time to turn over than normal protein, and it is said that the cycle becomes slower with aging, and when the turnover cycle becomes slow, the collagen itself is denatured. (Aging) advances, leading to a decrease in skin flexibility and elasticity (hardening). As collagen ages, its function as a structural support decreases, preventing the proliferation, differentiation, and migration of fibroblasts at the base of the skin, which support the turnover of the skin tissue, further increasing the turnover cycle of the skin tissue. It is thought to fall into a vicious cycle of slowing down.

  Based on this idea, various attempts have been made to promote collagen production. For example, reduction of collagen is supplemented with collagen (Patent Documents 1 and 2), for example, collagen metabolic activity (collagenase activity) is increased (Patent Documents 3 and 4), collagen production is promoted (Patent Documents 3 and 4) Patent Documents 5 and 6) can be cited.

Particularly in recent years, the search for plant-derived collagen-like substances and collagen production-promoting substances and the development of products containing them have been actively carried out by reducing the image of animal-derived raw materials (for example, Patent Documents 4 to 9). .
JP 2002-51734 A JP 2003-238597 A Japanese Patent Application Laid-Open No. 07-2699 Japanese Patent No. 3696464 Japanese Patent Laid-Open No. 2001-278783 JP 2003-121748 A JP 2003-206226 A JP 2003-277286 A JP 2006-8571 A

  However, many of the plant-derived collagen-like substances and collagen production-promoting substances described above are derived from herbal medicines and have dramatic effects, but on the other hand, there are problems of side effects due to the original medicinal effects and dose ranges that show appropriate effects. It was necessary to consider various points in use such as narrowness. In addition, many of the ingredients contain irritants, and it is necessary to consider various points in use, such as irritation to the skin and strong irritation (toxicity) to skin fibroblasts. It was.

  An object of the present invention is to provide a collagen production promoter that exhibits a gentle and reliable effect and is highly safe, and a cosmetic food and drink containing the same.

  As a result of intensive studies in order to achieve the above-mentioned problems, the present inventors have obtained an excellent collagen production promoting activity from an extract obtained from konjac koji, and by utilizing the extract obtained from konjac koji, The inventors have found that the problem can be solved and have reached the present invention.

  That is, the first aspect of the present invention is a collagen production promoter characterized by using konjac koji extract as an active ingredient. The second aspect of the present invention is a cosmetic food or drink characterized by blending the above-described collagen production promoter.

  INDUSTRIAL APPLICABILITY According to the present invention, there is provided a collagen production promoter having a konjac koji extract as an active ingredient, which shows an effect gently and surely, and has high safety, and a beauty food and drink containing the extract. can do.

Hereinafter, the present invention will be described in detail.
The konjac koji extract of the present invention can be obtained, for example, by the following method. Any kind of konjac can be used as a raw material as long as it is edible, such as a native species, a foreign species, and a new variety improved. Moreover, the konjac koji may be used as it is, or may be processed by operations such as drying, grinding, crushing, heating, and freezing. Moreover, as a raw material derived from these konjac meals, konjac powder produced as a by-product in the production process of konjac flour can be suitably used. Konjac powder is a resource that is discharged at 3000 to 4000 tons per year but is not used at all as a food, and is only used as a thickener for feed, concrete and the like.

  As the solvent used for obtaining the extract from the above konjac koji, any fraction may be used as long as the fraction having collagen production promoting activity is obtained from the konjac koji as a raw material and the effect of the present invention is not impaired. . As such, alcohols such as methanol, ethanol, propanol and butanol, polyhydric alcohols such as ethylene glycol, propylene glycol, butylene glycol and glycerin, ketones such as acetone and methyl ethyl ketone, esters such as methyl acetate and ethyl acetate , Ethers such as tetrahydrofuran and diethyl ether, halogenated hydrocarbons such as dichloromethane, dichloroethane and chloroform, aliphatic hydrocarbons such as hexane and pentane, aromatic hydrocarbons such as toluene, and polyethers such as polyethylene glycol And pyridines. One of these solvents may be used alone, or a plurality of solvents may be mixed and used.

  Of these, ethanol and hexane are preferred because they are used in foods. Moreover, when extracting with these organic solvents, in order to raise extraction efficiency, additives, such as water, a lecithin, a saponin, surfactant, etc. can be added in the range which does not impair the effect of this invention.

  In order to obtain the konjac koji extract used in the present invention, in addition to the above-described extraction with an organic solvent, a supercritical extraction method, which has attracted attention in recent years, can also be used. In the supercritical extraction method, for example, supercritical carbon dioxide can be used as an extraction solvent.

  The konjac koji extract thus obtained may be used as it is as the collagen production promoter of the present invention, and is concentrated, decolored, desalted, distributed, powdered as long as the effects of the present invention are not impaired. It is good also as what gave the process of chemical conversion etc., and such a thing is contained in a konjac koji extract in this invention. For example, the solvent may be distilled off with a rotary evaporator to increase the solid content, or the colored component may be removed by activated carbon treatment, or liquid-liquid distribution between the aqueous layer and the organic solvent layer Thus, the water-soluble component may be removed. Further, those extracts or processed products may be powdered by adding excipients such as dextrin and lactose.

  The konjac koji extract obtained as described above (including its concentrate, decolorized product, desalted product, distributed purified product, and powdered product) has an activity to promote collagen production. By orally ingesting the collagen production promoter of the present invention, one or more of skin flexibility, elasticity, keratin turnover, and collagen turnover can be improved.

  The cosmetic food / beverage product of the present invention contains the above-described collagen production promoter and refers to foods, beverages, luxury products, supplements, and the like taken orally. The form is not particularly limited, and can be a main dish such as breads and noodles, can be a side dish such as cheese, ham, wiener, processed seafood, beverages such as fruit juice drinks, carbonated drinks, milk drinks, Favorable items such as cookies, cakes, jelly, pudding, candy, and yogurt can be used. Moreover, the form as a supplement is not particularly limited, and can be in the form of a tablet, capsule, soft capsule, or energy drink.

  The intake of the collagen production promoter of the present invention and the cosmetic food and drink of the present invention is not particularly limited, but is preferably 20 mg to 50 g per day in terms of konjac extract. Furthermore, it is preferable that it is 30 mg-200 mg. If the intake is less than 20 mg, it is not sufficient to develop the collagen production promoting activity, and if it is ingested more than 50 g, the effect is no longer increased and only excreted.

  The konjac koji extract used in the present invention has various effects in addition to the collagen production promoting action. The main effects include skin moisturizing improvement, skin whitening action, hair and nail gloss improvement, hair loss prevention, carcinogenesis prevention, active oxygen removal, slimming action, and bowel movement improvement.

  In addition, a collagen production promoter containing the konjac koji extract of the present invention as an active ingredient and a cosmetic food and drink blended with the extract may be mixed with various functional ingredients in order to promote the effect. it can. Examples include vitamins, collagen, squalane, soybean lecithin, egg yolk lecithin, niacin, niacinamide, hyaluronic acid, placenta extract, sorbitol, chitin, chitosan, and various plant extracts. These mixing amounts are not limited as long as the effects of the present invention are not impaired.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to this.

Example 1 [Production 1 of collagen production promoter]
1 kg of konjac powder was charged into a stirring tank, 2 L of ethanol was added thereto, and the mixture was stirred at room temperature for 2 hours. Thereafter, the extract and the residue were separated by filtration. The extract was concentrated with an evaporator to obtain 10.7 g of a brownish waxy concentrate. 10 g of the waxy concentrate is redissolved in ethanol, 20 g of cyclodextrin is added thereto, and after mixing well, concentration, drying and crushing are performed to obtain 28 g of a powdered product of konjac koji extract. It was set as a collagen production promoter.

Example 2 [Production of collagen production promoter 2]
1 kg of konjac powder was charged into a stirring tank, 2 L of ethanol was added thereto, and the mixture was stirred at room temperature for 2 hours. Thereafter, the extract and the residue were separated by filtration. The extract was concentrated with an evaporator to obtain 11.0 g of a brownish waxy concentrate. 10.0 g of waxy concentrate was redissolved in 40.0 mL of ethanol, introduced into 100 mL of water with stirring, and stirred as it was in a dispersed state. The sodium chloride 20.0g was introduce | transduced there, and also it stirred for 10 minutes. Stirring was stopped and the mixture was allowed to stand for 1 hour while boiling on a hot plate. The upper black-brown oil layer was separated with a separatory funnel, and 40.0 mL of ethanol was again added and dissolved. This was concentrated with an evaporator and dried to obtain 7.7 g of a brown waxy concentrate. 7 g of the waxy concentrate is redissolved in ethanol, and 14 g of cyclodextrin is added thereto and mixed well, followed by concentration, drying and crushing to obtain 20 g of a konjac koji extract powder. It was set as a collagen production promoter.

Example 3 [Cosmetology food and drink; production of drink type]
The beauty drink which is one aspect | mode of the beauty food-drinks of this invention was manufactured with the manufacturing method shown next.

Ingredient Compounding amount (in 100 mL)
1) 50 mg of the collagen production promoter obtained in Example 2
2) Honey (Api) 320mg
3) Cyclodextrin (Shisui Minato Sugar) 600mg
4) Reduced maltose syrup (Nikken Kasei) appropriate amount 5) Citric acid (Fuso Chemical) appropriate amount 6) Sodium benzoate (Morimura Corporation) Appropriate amount 7) Xanthan gum (San-Eigen FFI) Appropriate amount 8) Grapefruit flavor (Fuji fragrance) ) Appropriate amount 9) Water Residue Manufacturing method: 1) to 8) were sequentially added to 9), mixed well with a mixer, and then filled with 50 mL / bottle.

Example 4 [Cosmetology food and drink; manufacture of tablet type]
The cosmetic tablet which is one aspect | mode of the beauty food-drinks of this invention was manufactured with the manufacturing method shown next.

Ingredient Compounding amount (in 100g)
1) Collagen production promoter obtained in Example 2 2000 mg
2) Maltitol (Nikken Kasei) 50.0g
3) Crystalline cellulose (Nihon Evolution Co., Ltd.) 31.0g
4) Sucrose fatty acid ester (Mitsubishi Chemical Foods) 8.0g
5) Vitamin C (Maruzen Pharmaceutical) 10.0g
6) Reduced maltose starch syrup (Niken Kasei) Appropriate amount 7) Citric acid (Fuso Chemical) Appropriate amount 8) Lemon flavor (Fuji fragrance) Appropriate amount 9) Lime flavor (Fuji fragrance) Appropriate amount Manufacturing method: 1) to 9) are mixed uniformly. After granulating it by a conventional method, it was tableted at 0.5 g / tablet.

Example 5 [Evaluation of cytotoxicity (cell experiment)]
In order to use for cell experiments, 0.2 g of the collagen production promoter obtained in each of Examples 1 and 2 was suspended in 60 mL of water, autoclaved at 121 ° C. for 2 hours, and the treatment liquid was centrifuged. The clear liquid was filtered through a membrane filter having a pore size of 0.2 μm to obtain about 60 mL of clear filtrates (Konjac koji extract hot water treated products 1 and 2). Cytotoxicity was examined by the following method using samples obtained by diluting these konjac koji extract hydrothermally treated products 1 and 2 with PBS (-) to 2, 5, and 10 volumes. In addition, as a comparative example, crude drug A (Korean ginseng) ethanol extract, crude drug B (Paffia ginseng) ethanol extract, 0.2 g of each was subjected to the same treatment (herbal medicine extract hot water treated products A and B) A similar test was conducted for.

  Normal human skin fibroblasts (NHDF: manufactured by Kurabo Industries) are seeded at a rate of 20000 cells / well in a 96-well plate, in a dedicated medium containing 2% fetal bovine serum (FBS) at 37 ° C. under 5% carbon dioxide gas. Cultured for 24 hours. After culturing for 24 hours, the cells were washed twice with PBS (−) and cultured for 72 hours in the same dedicated medium supplemented with 1/10 volume of the sample. The number of viable cells before and after sample addition was measured with Cell Counting Kit-8 (Dojindo Laboratories) to determine the survival rate.

As is apparent from Table 1, the collagen production promoter of the present invention showed high safety (low cytotoxicity).

Example 6 [Evaluation of Collagen Production Promotion Action (Cell Experiment)]
In order to use for cell experiments, konjac koji extract hydrothermally treated products 1 and 2 obtained in the same manner as in Example 5 were diluted 5 to 10 times with PBS (−) as samples, and the following method was used. Thus, the collagen production promoting effect was examined.

  Normal human skin fibroblasts (NHDF: manufactured by Kurabo Industries) are seeded at a rate of 20000 cells / well in a 96-well plate, in a dedicated medium containing 2% fetal bovine serum (FBS) at 37 ° C. under 5% carbon dioxide gas. Cultured for 24 hours. After culturing for 24 hours, the cells were washed twice with PBS (−), and cultured under the same conditions for 66 hours in a dedicated medium without FBS supplemented with 1/10 volume of the sample. The amount of collagen in the culture supernatant was measured with Procollagen type I-peptide (PIP) EIA Kit (TAKARA). Evaluation was made at a ratio where the amount of collagen produced in the case of adding PBS (-) as a control was 100%. In addition, the same test was done also about the crude drug extract hot-water processed goods A and B used in Example 5 as a comparative example.

As is clear from Table 2, the collagen production promoter of the present invention showed a strong collagen production promoting action, and the dose range was also relatively wide.

Example 7 [Confirmation of skin metabolism promoting effect]
Wistar rats (male, 4 weeks old, 3 animals / group) were bred for 3 weeks on a diet containing 6% protein, and used as a simulated aging model. In this rat, the collagen production promoter (powder product) obtained in Examples 1 and 2 was suspended in water, and 8.4 mg per 1 kg body weight was forcibly orally ingested once a day for 5 consecutive weeks. It was. The body hair on the back of the rat was removed 3 weeks after sample intake, 5% dansyl chloride / acetone solution was applied to the skin, and the number of days required for disappearance of dansyl chloride was observed and measured by UV irradiation.

  As is clear from FIG. 1, it was suggested that the collagen production promoter of the present invention increases the rate of skin metabolism.

Example 8 [Evaluation of Collagen Production Promoting Action (Animal Experiment)]
Wistar rats (male, 4 weeks old, 3 animals / group) were bred for 3 weeks on a diet containing 6% protein, and used as a simulated aging model. In this rat, the collagen production promoter (powder product) obtained in Examples 1 and 2 was suspended in water, and 8.4 mg per 1 kg body weight was forcibly orally ingested once a day for 5 consecutive weeks. It was. After the end of the sample intake period, the hair on the back of the rat was removed, and the skin was excised into a 4 × 5 cm rectangle together with the subcutaneous tissue. After measuring the weight of the extracted skin, 2 g of the skin was weighed, and 10 mL of a 0.15 M sodium chloride aqueous solution was added to sufficiently homogenize. After overnight shaking (extraction) under refrigeration (4 ° C.), the extracted supernatant was recovered by ultracentrifugation (40000 G × 1 hour, 4 ° C.). For precipitation, 0.5 M sodium chloride + 0.5 M 10 mL of acid aqueous solution (pH 3.6) was added, and the same extraction operation was performed. The extracted supernatants were combined and dialyzed (desalted) with a dialysis membrane having a molecular weight cut off of 8000. The dialyzed internal solution was concentrated to 2 mL with a rotary evaporator, 3 mL of 10N hydrochloric acid was added thereto, and hydrolysis treatment was performed at 110 ° C. for 24 hours under a hermetic stopper. After neutralizing the pH of the treatment liquid, the amount of hydroxyproline in soluble collagen contained in the skin was measured by high performance liquid chromatography (Amino Acid Analysis System manufactured by Shimadzu Corporation).

As is clear from Table 3, the collagen production promoter of the present invention increased skin weight and skin soluble collagen content, suggesting that it has a strong collagen production promoting action.

Example 9 [Confirmation of wrinkle improvement effect in human]
15 males and females aged 32 to 58 years with wrinkles on the face skin as the main symptom, 15 at a time, cosmetic food and drink of Example 4; tablet type intake group and placebo group (collagen production from the composition of the tablet) (Without accelerator). Food / beverage products for beauty; 3 tablets of each tablet type were ingested after each meal, and the effect of symptom improvement was evaluated 2 months after the start of ingestion. The improvement effect on wrinkles was observed by visually observing the condition of the wrinkles at the corners of the eyes, “improved: wrinkles became less noticeable”, “slightly improved: wrinkles became less noticeable”, and “no effect: no change” Each stage was evaluated and the number of subjects for each evaluation was counted.

As is apparent from the results in Table 4, the intake of the cosmetic food and drink of the present invention showed a significant effect on wrinkle improvement.

The result of having observed the metabolic rate of the back skin in the pseudo-aging rat which ingested the collagen production promoter from the disappearance days of the dansyl chloride applied to the back skin is shown.

Claims (2)

A collagen production promoter comprising konjac koji extract as an active ingredient. A cosmetic food or drink comprising the collagen production promoter according to claim 1.