JP2007533308A5 - - Google Patents
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- Publication number
- JP2007533308A5 JP2007533308A5 JP2007507422A JP2007507422A JP2007533308A5 JP 2007533308 A5 JP2007533308 A5 JP 2007533308A5 JP 2007507422 A JP2007507422 A JP 2007507422A JP 2007507422 A JP2007507422 A JP 2007507422A JP 2007533308 A5 JP2007533308 A5 JP 2007533308A5
- Authority
- JP
- Japan
- Prior art keywords
- cleavage site
- dna
- type
- selectable marker
- cleavage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000003776 cleavage reaction Methods 0.000 claims 98
- 230000007017 scission Effects 0.000 claims 98
- 108020004414 DNA Proteins 0.000 claims 57
- 239000003550 marker Substances 0.000 claims 35
- 102000053602 DNA Human genes 0.000 claims 25
- 238000000034 method Methods 0.000 claims 25
- 239000013598 vector Substances 0.000 claims 14
- 108010042407 Endonucleases Proteins 0.000 claims 12
- 102000004533 Endonucleases Human genes 0.000 claims 12
- 239000000203 mixture Substances 0.000 claims 12
- 108091008146 restriction endonucleases Proteins 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 108091026890 Coding region Proteins 0.000 claims 2
- 206010059866 Drug resistance Diseases 0.000 claims 2
- DGMPVYSXXIOGJY-UHFFFAOYSA-N Fusaric acid Chemical compound CCCCC1=CC=C(C(O)=O)N=C1 DGMPVYSXXIOGJY-UHFFFAOYSA-N 0.000 claims 2
- 108010030975 Polyketide Synthases Proteins 0.000 claims 2
- 239000013599 cloning vector Substances 0.000 claims 2
- ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 claims 1
- ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 claims 1
- 102000012410 DNA Ligases Human genes 0.000 claims 1
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 206010020751 Hypersensitivity Diseases 0.000 claims 1
- 101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- 239000004098 Tetracycline Substances 0.000 claims 1
- 208000026935 allergic disease Diseases 0.000 claims 1
- 230000003172 anti-dna Effects 0.000 claims 1
- 101150102092 ccdB gene Proteins 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000009610 hypersensitivity Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 101150025220 sacB gene Proteins 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 229960002180 tetracycline Drugs 0.000 claims 1
- 229930101283 tetracycline Natural products 0.000 claims 1
- 235000019364 tetracycline Nutrition 0.000 claims 1
- 150000003522 tetracyclines Chemical class 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/820,975 US20050227316A1 (en) | 2004-04-07 | 2004-04-07 | Synthetic genes |
| PCT/US2005/011452 WO2005103279A2 (en) | 2004-04-07 | 2005-04-05 | Synthetic genes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007533308A JP2007533308A (ja) | 2007-11-22 |
| JP2007533308A5 true JP2007533308A5 (https=) | 2008-05-15 |
Family
ID=35061024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007507422A Withdrawn JP2007533308A (ja) | 2004-04-07 | 2005-04-05 | 合成遺伝子 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20050227316A1 (https=) |
| EP (1) | EP1740713A4 (https=) |
| JP (1) | JP2007533308A (https=) |
| WO (1) | WO2005103279A2 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2608636C (en) * | 2005-05-17 | 2015-02-10 | Frank Koentgen | Sequential cloning system |
| US7805252B2 (en) * | 2005-08-16 | 2010-09-28 | Dna Twopointo, Inc. | Systems and methods for designing and ordering polynucleotides |
| US8153598B2 (en) * | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
| US20070148681A1 (en) * | 2005-12-16 | 2007-06-28 | Gene Oracle, Inc. | Gene synthesis kit |
| US8053191B2 (en) * | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| JP2010528608A (ja) * | 2007-06-01 | 2010-08-26 | 454 ライフ サイエンシーズ コーポレイション | 複合的な混合物から個々の試料を特定するためのシステムおよび方法 |
| JP5733784B2 (ja) * | 2009-09-24 | 2015-06-10 | 国立大学法人埼玉大学 | cDNA/mRNA−タンパク質連結体の効率的合成法 |
| BR112013007441A2 (pt) | 2010-09-28 | 2017-07-04 | Univ California | produção de alfa-olefinas usando sintases de policetídeo |
| WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
| CN103502448B (zh) | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
| LT2944693T (lt) * | 2011-08-26 | 2019-08-26 | Gen9, Inc. | Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| CA2871505C (en) | 2012-04-24 | 2021-10-12 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| IL236303B (en) | 2012-06-25 | 2022-07-01 | Gen9 Inc | Methods for high-throughput nucleic acid assembly and sequencing |
| CA3219413A1 (en) | 2014-05-16 | 2015-11-19 | Illumina, Inc. | Nucleic acid synthesis techniques |
| EP3826762A1 (en) * | 2018-07-23 | 2021-06-02 | DNA Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| WO2021241593A1 (ja) * | 2020-05-26 | 2021-12-02 | Spiber株式会社 | マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5824513A (en) * | 1991-01-17 | 1998-10-20 | Abbott Laboratories | Recombinant DNA method for producing erythromycin analogs |
| US5552278A (en) * | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US6358712B1 (en) * | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
| US6753173B1 (en) * | 1999-02-09 | 2004-06-22 | Board Of Trustees Of The Leland Stanford Junior University | Methods to mediate polyketide synthase module effectiveness |
| US7001748B2 (en) * | 1999-02-09 | 2006-02-21 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of making polyketides using hybrid polyketide synthases |
| US20020025561A1 (en) * | 2000-04-17 | 2002-02-28 | Hodgson Clague Pitman | Vectors for gene-self-assembly |
| IL156557A0 (en) * | 2000-12-22 | 2004-01-04 | Janssen Pharmaceutica Nv | Bax-responsive genes for drug target identification in yeast and fungi |
| EP1231220B1 (en) * | 2001-02-09 | 2009-05-13 | Claude Négrier | Modified factor VIII cDNa and its use for the production of factor VIII |
| US20030087254A1 (en) * | 2001-04-05 | 2003-05-08 | Simon Delagrave | Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics |
| AU2003213757A1 (en) * | 2002-03-04 | 2003-09-22 | Brown University Research Foundation | Methods to mediate polyketide synthase module effectiveness |
| JP2006517090A (ja) * | 2002-09-26 | 2006-07-20 | コーサン バイオサイエンシーズ, インコーポレイテッド | 合成遺伝子 |
| US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
-
2004
- 2004-04-07 US US10/820,975 patent/US20050227316A1/en not_active Abandoned
-
2005
- 2005-04-05 JP JP2007507422A patent/JP2007533308A/ja not_active Withdrawn
- 2005-04-05 WO PCT/US2005/011452 patent/WO2005103279A2/en not_active Ceased
- 2005-04-05 EP EP05767675A patent/EP1740713A4/en not_active Withdrawn
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