JP2007533308A - 合成遺伝子 - Google Patents

合成遺伝子 Download PDF

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Publication number
JP2007533308A
JP2007533308A JP2007507422A JP2007507422A JP2007533308A JP 2007533308 A JP2007533308 A JP 2007533308A JP 2007507422 A JP2007507422 A JP 2007507422A JP 2007507422 A JP2007507422 A JP 2007507422A JP 2007533308 A JP2007533308 A JP 2007533308A
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Prior art keywords
cleavage site
site
dna
vector
sequence
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Japanese (ja)
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JP2007533308A5 (https=
Inventor
ダニエル サンティ,
サラ ジェイ. コドゥマル,
ラルフ シー. ライド,
ケダル ジー. パテール,
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コーサン バイオサイエンシーズ, インコーポレイテッド
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Publication of JP2007533308A publication Critical patent/JP2007533308A/ja
Publication of JP2007533308A5 publication Critical patent/JP2007533308A5/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2007507422A 2004-04-07 2005-04-05 合成遺伝子 Withdrawn JP2007533308A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/820,975 US20050227316A1 (en) 2004-04-07 2004-04-07 Synthetic genes
PCT/US2005/011452 WO2005103279A2 (en) 2004-04-07 2005-04-05 Synthetic genes

Publications (2)

Publication Number Publication Date
JP2007533308A true JP2007533308A (ja) 2007-11-22
JP2007533308A5 JP2007533308A5 (https=) 2008-05-15

Family

ID=35061024

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007507422A Withdrawn JP2007533308A (ja) 2004-04-07 2005-04-05 合成遺伝子

Country Status (4)

Country Link
US (1) US20050227316A1 (https=)
EP (1) EP1740713A4 (https=)
JP (1) JP2007533308A (https=)
WO (1) WO2005103279A2 (https=)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539766A (ja) * 2005-05-17 2008-11-20 オズジーン プロプライアタリー リミテッド 連続的クローニング系
JP2011087573A (ja) * 2009-09-24 2011-05-06 Saitama Univ cDNA/mRNA−タンパク質連結体の効率的合成法
JP2013544500A (ja) * 2010-09-28 2013-12-19 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ポリケチドシンターゼを用いたα−オレフィンの生成
JP2015523866A (ja) * 2012-06-25 2015-08-20 ジェン9・インコーポレイテッドGen9,INC. 核酸アセンブリおよび高処理シークエンシングのための方法
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US10202608B2 (en) 2006-08-31 2019-02-12 Gen9, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US10308931B2 (en) 2012-03-21 2019-06-04 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US11084014B2 (en) 2010-11-12 2021-08-10 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Spiber株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法
US11702662B2 (en) 2011-08-26 2023-07-18 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7805252B2 (en) * 2005-08-16 2010-09-28 Dna Twopointo, Inc. Systems and methods for designing and ordering polynucleotides
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
US20070148681A1 (en) * 2005-12-16 2007-06-28 Gene Oracle, Inc. Gene synthesis kit
JP2010528608A (ja) * 2007-06-01 2010-08-26 454 ライフ サイエンシーズ コーポレイション 複合的な混合物から個々の試料を特定するためのシステムおよび方法
CA3219413A1 (en) 2014-05-16 2015-11-19 Illumina, Inc. Nucleic acid synthesis techniques
EP3826762A1 (en) * 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824513A (en) * 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US6358712B1 (en) * 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US6753173B1 (en) * 1999-02-09 2004-06-22 Board Of Trustees Of The Leland Stanford Junior University Methods to mediate polyketide synthase module effectiveness
US7001748B2 (en) * 1999-02-09 2006-02-21 The Board Of Trustees Of The Leland Stanford Junior University Methods of making polyketides using hybrid polyketide synthases
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
IL156557A0 (en) * 2000-12-22 2004-01-04 Janssen Pharmaceutica Nv Bax-responsive genes for drug target identification in yeast and fungi
EP1231220B1 (en) * 2001-02-09 2009-05-13 Claude Négrier Modified factor VIII cDNa and its use for the production of factor VIII
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
AU2003213757A1 (en) * 2002-03-04 2003-09-22 Brown University Research Foundation Methods to mediate polyketide synthase module effectiveness
JP2006517090A (ja) * 2002-09-26 2006-07-20 コーサン バイオサイエンシーズ, インコーポレイテッド 合成遺伝子
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539766A (ja) * 2005-05-17 2008-11-20 オズジーン プロプライアタリー リミテッド 連続的クローニング系
US10202608B2 (en) 2006-08-31 2019-02-12 Gen9, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
JP2011087573A (ja) * 2009-09-24 2011-05-06 Saitama Univ cDNA/mRNA−タンパク質連結体の効率的合成法
JP2013544500A (ja) * 2010-09-28 2013-12-19 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ポリケチドシンターゼを用いたα−オレフィンの生成
US9856461B2 (en) 2010-09-28 2018-01-02 The Regents Of The University Of California Producing alpha-olefins using polyketide synthases
US10982208B2 (en) 2010-11-12 2021-04-20 Gen9, Inc. Protein arrays and methods of using and making the same
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis
US11084014B2 (en) 2010-11-12 2021-08-10 Gen9, Inc. Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US11702662B2 (en) 2011-08-26 2023-07-18 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US10308931B2 (en) 2012-03-21 2019-06-04 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10927369B2 (en) 2012-04-24 2021-02-23 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US11072789B2 (en) 2012-06-25 2021-07-27 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
JP2019150030A (ja) * 2012-06-25 2019-09-12 ギンゴー バイオワークス, インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
JP7009409B2 (ja) 2012-06-25 2022-01-25 ジェン9・インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
JP2022050592A (ja) * 2012-06-25 2022-03-30 ジェン9・インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
JP7322202B2 (ja) 2012-06-25 2023-08-07 ジェン9・インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
JP2015523866A (ja) * 2012-06-25 2015-08-20 ジェン9・インコーポレイテッドGen9,INC. 核酸アセンブリおよび高処理シークエンシングのための方法
US12241057B2 (en) 2012-06-25 2025-03-04 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Spiber株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法

Also Published As

Publication number Publication date
US20050227316A1 (en) 2005-10-13
EP1740713A4 (en) 2009-11-04
WO2005103279A2 (en) 2005-11-03
EP1740713A2 (en) 2007-01-10
WO2005103279A3 (en) 2009-04-23

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