JP2021523704A5 - - Google Patents
Info
- Publication number
- JP2021523704A5 JP2021523704A5 JP2020562111A JP2020562111A JP2021523704A5 JP 2021523704 A5 JP2021523704 A5 JP 2021523704A5 JP 2020562111 A JP2020562111 A JP 2020562111A JP 2020562111 A JP2020562111 A JP 2020562111A JP 2021523704 A5 JP2021523704 A5 JP 2021523704A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- polynucleotides
- target polynucleotide
- sample
- adapter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1808554.8A GB201808554D0 (en) | 2018-05-24 | 2018-05-24 | Method |
| GB1808554.8 | 2018-05-24 | ||
| PCT/GB2019/051444 WO2019224560A1 (en) | 2018-05-24 | 2019-05-24 | Method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021523704A JP2021523704A (ja) | 2021-09-09 |
| JP2021523704A5 true JP2021523704A5 (https=) | 2022-04-25 |
| JP7365363B2 JP7365363B2 (ja) | 2023-10-19 |
Family
ID=62812420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020562111A Active JP7365363B2 (ja) | 2018-05-24 | 2019-05-24 | 方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20210198732A1 (https=) |
| EP (2) | EP4549584A3 (https=) |
| JP (1) | JP7365363B2 (https=) |
| CN (1) | CN112105744A (https=) |
| AU (1) | AU2019274949B2 (https=) |
| CA (1) | CA3096856A1 (https=) |
| GB (1) | GB201808554D0 (https=) |
| WO (1) | WO2019224560A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11046995B2 (en) | 2016-08-16 | 2021-06-29 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (FLASH) |
| EP3731959B1 (en) | 2017-12-29 | 2025-10-08 | Clear Labs, Inc. | Automated priming and library loading device |
| TW202039845A (zh) * | 2018-12-20 | 2020-11-01 | 北京大學 | 使用加標籤的嚮導rna構建體進行高效基因篩選的組合物和方法 |
| CN112029838B (zh) * | 2020-07-23 | 2022-07-12 | 东南大学 | 一种用于DNA均相检测的CRISPR/Cas9分型PCR方法及其应用 |
| LT4211260T (lt) | 2020-09-11 | 2025-06-25 | New England Biolabs, Inc. | Imobilizuotų fermentų taikymas nanoporų bibliotekos konstravimui |
| WO2022243437A1 (en) * | 2021-05-19 | 2022-11-24 | KWS SAAT SE & Co. KGaA | Sample preparation with oppositely oriented guide polynucleotides |
| WO2023107899A2 (en) * | 2021-12-07 | 2023-06-15 | Caribou Biosciences, Inc. | A method of capturing crispr endonuclease cleavage products |
| CN114457145B (zh) * | 2022-01-29 | 2023-08-11 | 成都齐碳科技有限公司 | 用于表征靶多核苷酸测序的接头、构建体、方法和应用 |
| CN114921533A (zh) * | 2022-05-05 | 2022-08-19 | 成都齐碳科技有限公司 | 用于表征目标多核苷酸的方法及衔接体 |
| CN114921535B (zh) * | 2022-05-19 | 2023-05-23 | 四川大学华西医院 | 一种rna长片段靶向测序方法 |
| CN114921534B (zh) * | 2022-05-19 | 2023-05-30 | 四川大学华西医院 | 一种高靶向效率和数据产量的rna长片段靶向测序方法 |
| WO2024138517A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大生命科学研究院 | 提升测序通量的文库接头设计 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| FR2861405A1 (fr) * | 2003-10-24 | 2005-04-29 | Centre Nat Rech Scient | METHODE DE PRODUCTION D'ADNc PLEINE LONGUEUR PAR LIGATURATION D'ADAPTATEUR A EXTREMITE COHESIVE |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| US20110121840A1 (en) | 2007-02-20 | 2011-05-26 | Gurdial Singh Sanghera | Lipid Bilayer Sensor System |
| KR20110125226A (ko) | 2009-01-30 | 2011-11-18 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 혼성화 링커 |
| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| WO2015150786A1 (en) | 2014-04-04 | 2015-10-08 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3183358B1 (en) * | 2014-08-19 | 2020-10-07 | President and Fellows of Harvard College | Rna-guided systems for probing and mapping of nucleic acids |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| WO2017044843A1 (en) * | 2015-09-11 | 2017-03-16 | The General Hospital Corporation | Full interrogation of nuclease dsbs and sequencing (find-seq) |
| WO2017162754A1 (en) * | 2016-03-22 | 2017-09-28 | Vib Vzw | Means and methods for amplifying nucleotide sequences |
| US11046995B2 (en) * | 2016-08-16 | 2021-06-29 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (FLASH) |
| GB201616590D0 (en) * | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
-
2018
- 2018-05-24 GB GBGB1808554.8A patent/GB201808554D0/en not_active Ceased
-
2019
- 2019-05-24 US US17/057,863 patent/US20210198732A1/en active Pending
- 2019-05-24 CA CA3096856A patent/CA3096856A1/en active Pending
- 2019-05-24 WO PCT/GB2019/051444 patent/WO2019224560A1/en not_active Ceased
- 2019-05-24 EP EP25161085.3A patent/EP4549584A3/en active Pending
- 2019-05-24 AU AU2019274949A patent/AU2019274949B2/en active Active
- 2019-05-24 CN CN201980029513.6A patent/CN112105744A/zh active Pending
- 2019-05-24 EP EP19727476.4A patent/EP3802862A1/en not_active Ceased
- 2019-05-24 JP JP2020562111A patent/JP7365363B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2021523704A5 (https=) | ||
| US12110545B2 (en) | Methods of assessing nuclease cleavage | |
| US8003322B2 (en) | Method of amplifying a target nucleic acid by rolling circle amplification | |
| US20200291452A1 (en) | Method | |
| US11697836B2 (en) | Normalization of NGS library concentration | |
| US7601499B2 (en) | Paired end sequencing | |
| US12571016B2 (en) | Methods and compositions for nucleic acid amplification | |
| JP2026035740A (ja) | 核酸配列決定のための方法および試薬、ならびに関連する用途 | |
| WO2008051530A3 (en) | Polymerase enzymes and reagents for enhanced nucleic acid sequencing | |
| EP2242855A1 (en) | Paired end sequencing | |
| US20230287477A1 (en) | Methods and compositions for recombinase-mediated selective cleavage of nucleic acids | |
| US20200109426A1 (en) | Programmable nucleic acid synthesis cascade | |
| WO2016135300A1 (en) | Efficiency improving methods for gene library generation | |
| JP4535615B2 (ja) | 核酸増幅のモニター及び検出のための蛍光定量法 | |
| KR20220084322A (ko) | 세포에서 하나 이상의 표적-특이적인 프로그램 가능한 뉴클레아제의 오프-타겟 활성을 프로파일링하는 진정한 편향되지 않은 시험관내 분석(abnoba-seq) | |
| JP2023539169A5 (https=) | ||
| US9150914B2 (en) | Methods and compositions for amplification and sequencing of difficult DNA templates | |
| US10059985B2 (en) | Method of amplifying telomere | |
| JP2022515466A (ja) | 標的化された相補的dna富化のための方法 | |
| US20240076653A1 (en) | Method for constructing multiplex pcr library for high-throughput targeted sequencing | |
| Ukai et al. | A new technique to prevent self-ligation of DNA | |
| KR20170020091A (ko) | shRNA 발현 카세트의 제조방법, 그 방법에 의해 제조된 shRNA 발현 카세트 및 이를 포함하는 라이브러리 | |
| JP2016136851A (ja) | ミスマッチエンドヌクレアーゼ活性を有するポリペプチドのゲノム編集への利用 | |
| CN119932155A (zh) | 用于靶向基因组富集的方法和试剂盒 | |
| JP2024501832A5 (https=) |