CN112105744A - 方法 - Google Patents

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Publication number
CN112105744A
CN112105744A CN201980029513.6A CN201980029513A CN112105744A CN 112105744 A CN112105744 A CN 112105744A CN 201980029513 A CN201980029513 A CN 201980029513A CN 112105744 A CN112105744 A CN 112105744A
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CN
China
Prior art keywords
polynucleotide
target
adaptor
effector protein
target polynucleotide
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Pending
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CN201980029513.6A
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English (en)
Chinese (zh)
Inventor
詹姆斯·爱德华·格拉哈姆
艾蒂安·雷蒙多
瑞贝卡·维多利亚·鲍恩
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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Publication date
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Publication of CN112105744A publication Critical patent/CN112105744A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN201980029513.6A 2018-05-24 2019-05-24 方法 Pending CN112105744A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1808554.8A GB201808554D0 (en) 2018-05-24 2018-05-24 Method
GB1808554.8 2018-05-24
PCT/GB2019/051444 WO2019224560A1 (en) 2018-05-24 2019-05-24 Method

Publications (1)

Publication Number Publication Date
CN112105744A true CN112105744A (zh) 2020-12-18

Family

ID=62812420

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980029513.6A Pending CN112105744A (zh) 2018-05-24 2019-05-24 方法

Country Status (8)

Country Link
US (1) US20210198732A1 (https=)
EP (2) EP4549584A3 (https=)
JP (1) JP7365363B2 (https=)
CN (1) CN112105744A (https=)
AU (1) AU2019274949B2 (https=)
CA (1) CA3096856A1 (https=)
GB (1) GB201808554D0 (https=)
WO (1) WO2019224560A1 (https=)

Cited By (4)

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CN114457145A (zh) * 2022-01-29 2022-05-10 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
CN114921535A (zh) * 2022-05-19 2022-08-19 四川大学华西医院 一种rna长片段靶向测序方法
CN114921534A (zh) * 2022-05-19 2022-08-19 四川大学华西医院 一种高靶向效率和数据产量的rna长片段靶向测序方法
WO2024138517A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 提升测序通量的文库接头设计

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US11046995B2 (en) 2016-08-16 2021-06-29 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (FLASH)
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
TW202039845A (zh) * 2018-12-20 2020-11-01 北京大學 使用加標籤的嚮導rna構建體進行高效基因篩選的組合物和方法
CN112029838B (zh) * 2020-07-23 2022-07-12 东南大学 一种用于DNA均相检测的CRISPR/Cas9分型PCR方法及其应用
LT4211260T (lt) 2020-09-11 2025-06-25 New England Biolabs, Inc. Imobilizuotų fermentų taikymas nanoporų bibliotekos konstravimui
WO2022243437A1 (en) * 2021-05-19 2022-11-24 KWS SAAT SE & Co. KGaA Sample preparation with oppositely oriented guide polynucleotides
WO2023107899A2 (en) * 2021-12-07 2023-06-15 Caribou Biosciences, Inc. A method of capturing crispr endonuclease cleavage products
CN114921533A (zh) * 2022-05-05 2022-08-19 成都齐碳科技有限公司 用于表征目标多核苷酸的方法及衔接体

Citations (4)

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CN107075546A (zh) * 2014-08-19 2017-08-18 哈佛学院董事及会员团体 用于对核酸探测并作图的rna‑引导的系统
WO2017162754A1 (en) * 2016-03-22 2017-09-28 Vib Vzw Means and methods for amplifying nucleotide sequences
WO2018035062A1 (en) * 2016-08-16 2018-02-22 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (flash)
WO2018060740A1 (en) * 2016-09-29 2018-04-05 Oxford Nanopore Technologies Limited Method for nucleic acid detection by guiding through a nanopore

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
FR2861405A1 (fr) * 2003-10-24 2005-04-29 Centre Nat Rech Scient METHODE DE PRODUCTION D'ADNc PLEINE LONGUEUR PAR LIGATURATION D'ADAPTATEUR A EXTREMITE COHESIVE
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2017044843A1 (en) * 2015-09-11 2017-03-16 The General Hospital Corporation Full interrogation of nuclease dsbs and sequencing (find-seq)

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
CN107075546A (zh) * 2014-08-19 2017-08-18 哈佛学院董事及会员团体 用于对核酸探测并作图的rna‑引导的系统
WO2017162754A1 (en) * 2016-03-22 2017-09-28 Vib Vzw Means and methods for amplifying nucleotide sequences
WO2018035062A1 (en) * 2016-08-16 2018-02-22 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (flash)
WO2018060740A1 (en) * 2016-09-29 2018-04-05 Oxford Nanopore Technologies Limited Method for nucleic acid detection by guiding through a nanopore

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114457145A (zh) * 2022-01-29 2022-05-10 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
WO2023143247A1 (zh) * 2022-01-29 2023-08-03 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
CN114457145B (zh) * 2022-01-29 2023-08-11 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
CN114921535A (zh) * 2022-05-19 2022-08-19 四川大学华西医院 一种rna长片段靶向测序方法
CN114921534A (zh) * 2022-05-19 2022-08-19 四川大学华西医院 一种高靶向效率和数据产量的rna长片段靶向测序方法
WO2024138517A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 提升测序通量的文库接头设计

Also Published As

Publication number Publication date
WO2019224560A1 (en) 2019-11-28
AU2019274949B2 (en) 2025-08-14
EP3802862A1 (en) 2021-04-14
US20210198732A1 (en) 2021-07-01
EP4549584A2 (en) 2025-05-07
JP2021523704A (ja) 2021-09-09
AU2019274949A1 (en) 2020-10-15
CA3096856A1 (en) 2019-11-28
JP7365363B2 (ja) 2023-10-19
GB201808554D0 (en) 2018-07-11
EP4549584A3 (en) 2025-07-30

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