JP2007526460A - 細胞集団のタンパク質発現プロファイルの作成のための分析用プラットフォームおよび方法 - Google Patents
細胞集団のタンパク質発現プロファイルの作成のための分析用プラットフォームおよび方法 Download PDFInfo
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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| JP2014516644A (ja) * | 2011-04-29 | 2014-07-17 | セブンス センス バイオシステムズ,インコーポレーテッド | 血斑または他の体液の収集および/または操作のためのデバイスおよび方法 |
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| US9775551B2 (en) | 2009-03-02 | 2017-10-03 | Seventh Sense Biosystems, Inc. | Devices and techniques associated with diagnostics, therapies, and other applications, including skin-associated applications |
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| WO2011008990A1 (en) | 2009-07-15 | 2011-01-20 | Prometheus Laboratories Inc. | Drug selection for gastric cancer therapy using antibody-based arrays |
| WO2008036802A2 (en) * | 2006-09-21 | 2008-03-27 | Prometheus Laboratories Inc. | Antibody-based arrays for detecting multiple signal transducers in rare circulating cells |
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| CN103399144B (zh) | 2008-02-25 | 2015-10-28 | 雀巢产品技术援助有限公司 | 用抗体阵列选择乳腺癌治疗药物 |
| WO2012046412A1 (ja) * | 2010-10-07 | 2012-04-12 | パナソニック株式会社 | プラズモンセンサ |
| US9719995B2 (en) | 2011-02-03 | 2017-08-01 | Pierian Holdings, Inc. | Drug selection for colorectal cancer therapy using receptor tyrosine kinase profiling |
| WO2013033623A1 (en) | 2011-09-02 | 2013-03-07 | Nestec S.A. | Profiling of signal pathway proteins to determine therapeutic efficacy |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001092870A2 (de) * | 2000-06-02 | 2001-12-06 | Zeptosens Ag | Kit und verfahren zur multianalytbestimmung |
| WO2002023199A2 (en) * | 2000-09-14 | 2002-03-21 | Reverse Proteomics Research Institute Co., Ltd. | Method, system, apparatus and device for discovering and preparing chemical compounds |
| WO2004009639A1 (ja) * | 2002-07-18 | 2004-01-29 | Cellfree Sciences Co., Ltd. | 単鎖抗体およびその利用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL317379A1 (en) * | 1994-05-27 | 1997-04-01 | Ciba Geigy Ag | Supercritically excited luminescence detecting process |
| EP1890130A3 (en) * | 1995-05-12 | 2008-02-27 | Novartis AG | Sensor platform for the parallel detection of a plurality of analytes using evanescently excited luminescenes |
| DE19628928A1 (de) * | 1996-07-18 | 1998-01-22 | Basf Ag | Feste Träger für analytische Meßverfahren, ein Verfahren zu ihrer Herstellung sowie ihre Verwendung |
| WO1998009156A1 (en) * | 1996-08-29 | 1998-03-05 | Novartis Ag | Optical chemical / biochemical sensor |
| US6087102A (en) * | 1998-01-07 | 2000-07-11 | Clontech Laboratories, Inc. | Polymeric arrays and methods for their use in binding assays |
| AU735887B2 (en) * | 1998-01-09 | 2001-07-19 | Phylogica Limited | Peptide detection method |
| US6197599B1 (en) * | 1998-07-30 | 2001-03-06 | Guorong Chin | Method to detect proteins |
| EP1112262B1 (en) * | 1998-09-09 | 2004-08-04 | Inflazyme Pharmaceuticals Ltd. | Substituted gamma-phenyl-delta-lactones and analogs thereof and uses related thereto |
| US20030027214A1 (en) * | 1999-02-17 | 2003-02-06 | Kamb Carl Alexander | Methods for substrate-ligand interaction screening |
| US6972198B2 (en) * | 1999-02-26 | 2005-12-06 | Cyclacel, Ltd. | Methods and compositions using protein binding partners |
| GB0007088D0 (en) * | 2000-03-23 | 2000-05-17 | Torsana A S | Detection of immunological memory determining the position of loci of pathologyand therapeutic t-cell conjugates |
| US7175811B2 (en) * | 2000-04-28 | 2007-02-13 | Edgelight Biosciences | Micro-array evanescent wave fluorescence detection device |
| EP1315968B1 (de) * | 2000-09-05 | 2008-02-13 | Bayer Technology Services GmbH | Verfahren zur abscheidung von mono- und mehrfachschichten von organophosphor- und -phosphonsäuren und deren salzen sowie deren verwendung |
| US7070740B1 (en) * | 2000-09-28 | 2006-07-04 | Beckman Coulter, Inc. | Method and apparatus for processing biomolecule arrays |
| US7332286B2 (en) * | 2001-02-02 | 2008-02-19 | University Of Pennsylvania | Peptide or protein microassay method and apparatus |
| US7465568B2 (en) * | 2002-04-26 | 2008-12-16 | Bristol-Myers Squibb Company | Essential fungal polynucleotides, polypeptides, and methods of use |
| ATE520027T1 (de) * | 2002-09-03 | 2011-08-15 | Bayer Technology Services Gmbh | Analytische plattform und nachweisverfahren mit gegebenenfalls nach fraktionierung in einer probe nachzuweisenden analyten als immobilisierten spezifischen bindungspartnern |
| US6956651B2 (en) * | 2002-09-07 | 2005-10-18 | Hilary S. Lackritz | Bioanalysis systems including optical integrated circuit |
-
2004
- 2004-03-03 US US10/598,418 patent/US20080020409A1/en not_active Abandoned
- 2004-03-03 CN CNA2004800422474A patent/CN1954213A/zh active Pending
- 2004-03-03 AU AU2004318038A patent/AU2004318038A1/en not_active Abandoned
- 2004-03-03 EP EP04716582A patent/EP1730530A1/en not_active Withdrawn
- 2004-03-03 JP JP2007501122A patent/JP2007526460A/ja active Pending
- 2004-03-03 WO PCT/EP2004/002127 patent/WO2005095965A1/en not_active Ceased
- 2004-03-03 CA CA002559803A patent/CA2559803A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001092870A2 (de) * | 2000-06-02 | 2001-12-06 | Zeptosens Ag | Kit und verfahren zur multianalytbestimmung |
| WO2002023199A2 (en) * | 2000-09-14 | 2002-03-21 | Reverse Proteomics Research Institute Co., Ltd. | Method, system, apparatus and device for discovering and preparing chemical compounds |
| WO2004009639A1 (ja) * | 2002-07-18 | 2004-01-29 | Cellfree Sciences Co., Ltd. | 単鎖抗体およびその利用 |
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| US9730624B2 (en) | 2009-03-02 | 2017-08-15 | Seventh Sense Biosystems, Inc. | Delivering and/or receiving fluids |
| US9775551B2 (en) | 2009-03-02 | 2017-10-03 | Seventh Sense Biosystems, Inc. | Devices and techniques associated with diagnostics, therapies, and other applications, including skin-associated applications |
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| US11177029B2 (en) | 2010-08-13 | 2021-11-16 | Yourbio Health, Inc. | Systems and techniques for monitoring subjects |
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| US10835163B2 (en) | 2011-04-29 | 2020-11-17 | Seventh Sense Biosystems, Inc. | Systems and methods for collecting fluid from a subject |
| US10188335B2 (en) | 2011-04-29 | 2019-01-29 | Seventh Sense Biosystems, Inc. | Plasma or serum production and removal of fluids under reduced pressure |
| US11253179B2 (en) | 2011-04-29 | 2022-02-22 | Yourbio Health, Inc. | Systems and methods for collection and/or manipulation of blood spots or other bodily fluids |
| JP2014516644A (ja) * | 2011-04-29 | 2014-07-17 | セブンス センス バイオシステムズ,インコーポレーテッド | 血斑または他の体液の収集および/または操作のためのデバイスおよび方法 |
| US10543310B2 (en) | 2011-12-19 | 2020-01-28 | Seventh Sense Biosystems, Inc. | Delivering and/or receiving material with respect to a subject surface |
| JP2017536106A (ja) * | 2014-06-03 | 2017-12-07 | 韓国科学技術院Korea Advanced Institute Of Science And Technology | シグナル伝達経路の活性化状態の分析方法およびこれを用いた個人向け治療剤の選定方法 |
| JP2020156490A (ja) * | 2014-06-03 | 2020-10-01 | 韓国科学技術院Korea Advanced Institute Of Science And Technology | シグナル伝達経路の活性化状態の分析方法およびこれを用いた個人向け治療剤の選定方法 |
Also Published As
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| EP1730530A1 (en) | 2006-12-13 |
| CN1954213A (zh) | 2007-04-25 |
| AU2004318038A1 (en) | 2005-10-13 |
| US20080020409A1 (en) | 2008-01-24 |
| WO2005095965A1 (en) | 2005-10-13 |
| CA2559803A1 (en) | 2005-10-13 |
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