JP2007516729A - 酵素消化による核酸の迅速な調製 - Google Patents

酵素消化による核酸の迅速な調製 Download PDF

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Publication number
JP2007516729A
JP2007516729A JP2006547582A JP2006547582A JP2007516729A JP 2007516729 A JP2007516729 A JP 2007516729A JP 2006547582 A JP2006547582 A JP 2006547582A JP 2006547582 A JP2006547582 A JP 2006547582A JP 2007516729 A JP2007516729 A JP 2007516729A
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Prior art keywords
enzyme solution
nucleic acid
solution
biological sample
binding
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JP2006547582A
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Japanese (ja)
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JP2007516729A5 (https=
Inventor
フキアン・チェン
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Sigma Aldrich Co LLC
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Sigma Aldrich Co LLC
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Publication of JP2007516729A publication Critical patent/JP2007516729A/ja
Publication of JP2007516729A5 publication Critical patent/JP2007516729A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2006547582A 2003-12-30 2004-12-30 酵素消化による核酸の迅速な調製 Withdrawn JP2007516729A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53362403P 2003-12-30 2003-12-30
PCT/US2004/043980 WO2005068662A1 (en) 2003-12-30 2004-12-30 Rapid preparation of nucleic acids by enzymatic digestion

Publications (2)

Publication Number Publication Date
JP2007516729A true JP2007516729A (ja) 2007-06-28
JP2007516729A5 JP2007516729A5 (https=) 2007-08-09

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ID=34794239

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JP2006547582A Withdrawn JP2007516729A (ja) 2003-12-30 2004-12-30 酵素消化による核酸の迅速な調製

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US (1) US20050164260A1 (https=)
EP (1) EP1709197A4 (https=)
JP (1) JP2007516729A (https=)
KR (1) KR20060129340A (https=)
IL (1) IL176609A0 (https=)
NO (1) NO20063278L (https=)
WO (1) WO2005068662A1 (https=)

Cited By (1)

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JP2013503639A (ja) * 2009-09-03 2013-02-04 ベクトン・ディキンソン・アンド・カンパニー 直接的な化学的溶解のための方法および組成物

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DE102005057334A1 (de) * 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
EP2281826B1 (en) * 2008-04-02 2016-02-17 Rbc Bioscience Corp. Reagents, chaotropic agents, kits and methods for isolating nucleic acids based on magnetic cellulose materials
WO2012044991A1 (en) 2010-09-30 2012-04-05 Phynexus, Inc. Purification of nucleic acids
US10072284B2 (en) 2012-06-21 2018-09-11 Monsanto Technology Llc Lysis buffer and methods for extraction of DNA from plant material
TWI555850B (zh) * 2012-08-09 2016-11-01 財團法人工業技術研究院 用於分離核酸的組合物及方法
CN103571824B (zh) * 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
WO2016183292A1 (en) * 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
WO2017118719A1 (en) * 2016-01-08 2017-07-13 Pathoquest Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in a cellular biological fluids
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US10428370B2 (en) 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
CA3036702A1 (en) * 2016-09-15 2018-03-22 Sun Genomics Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
TW202513803A (zh) * 2023-09-22 2025-04-01 南韓商西諾海利斯股份有限公司 用於純化或提取核酸的組合物及其用途

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US4843012A (en) * 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
GB8725606D0 (en) * 1987-11-02 1987-12-09 Soc D Etudes Prod Chimique Preparation polynucleotides
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
DE68914689T2 (de) * 1989-05-22 1994-10-27 Genetics Inst Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsäure und dadurch verbessertes verfahren.
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
SE9103215D0 (sv) * 1991-11-04 1991-11-04 Kabi Pharmacia Ab A method and device for dosing a liquid preparation
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5438129A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
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US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5660984A (en) * 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
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EP1007730A4 (en) * 1996-08-30 2004-04-28 Invitrogen Corp METHODS OF IDENTIFYING AND ISOLATING SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA
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Cited By (4)

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Publication number Priority date Publication date Assignee Title
JP2013503639A (ja) * 2009-09-03 2013-02-04 ベクトン・ディキンソン・アンド・カンパニー 直接的な化学的溶解のための方法および組成物
US10190152B2 (en) 2009-09-03 2019-01-29 Becton, Dickinson And Company Methods and compositions for direct chemical lysis
US10323267B2 (en) 2009-09-03 2019-06-18 Becton Dickinson And Company Methods and compositions for direct chemical lysis
US11434519B2 (en) 2009-09-03 2022-09-06 Becton, Dickinson And Company Methods and compositions for direct chemical lysis

Also Published As

Publication number Publication date
WO2005068662A1 (en) 2005-07-28
NO20063278L (no) 2006-09-29
EP1709197A4 (en) 2007-07-04
KR20060129340A (ko) 2006-12-15
IL176609A0 (en) 2006-10-31
US20050164260A1 (en) 2005-07-28
EP1709197A1 (en) 2006-10-11

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