KR20060129340A - 효소 소화에 의한 핵산의 신속한 제조 - Google Patents
효소 소화에 의한 핵산의 신속한 제조 Download PDFInfo
- Publication number
- KR20060129340A KR20060129340A KR1020067015339A KR20067015339A KR20060129340A KR 20060129340 A KR20060129340 A KR 20060129340A KR 1020067015339 A KR1020067015339 A KR 1020067015339A KR 20067015339 A KR20067015339 A KR 20067015339A KR 20060129340 A KR20060129340 A KR 20060129340A
- Authority
- KR
- South Korea
- Prior art keywords
- biological sample
- enzyme solution
- solution
- nucleic acid
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 83
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 83
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 83
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 238000001976 enzyme digestion Methods 0.000 title description 2
- 238000000034 method Methods 0.000 claims abstract description 73
- 239000012472 biological sample Substances 0.000 claims abstract description 59
- 239000000203 mixture Substances 0.000 claims abstract description 51
- 239000012530 fluid Substances 0.000 claims abstract description 21
- 239000006143 cell culture medium Substances 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims description 123
- 108090000790 Enzymes Proteins 0.000 claims description 123
- 229940088598 enzyme Drugs 0.000 claims description 123
- 108020004414 DNA Proteins 0.000 claims description 75
- 210000004027 cell Anatomy 0.000 claims description 59
- 230000027455 binding Effects 0.000 claims description 58
- 235000010335 lysozyme Nutrition 0.000 claims description 49
- 108010014251 Muramidase Proteins 0.000 claims description 47
- 102000016943 Muramidase Human genes 0.000 claims description 47
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 47
- 239000004325 lysozyme Substances 0.000 claims description 47
- 229960000274 lysozyme Drugs 0.000 claims description 47
- 239000003599 detergent Substances 0.000 claims description 37
- 229910052751 metal Inorganic materials 0.000 claims description 34
- 239000002184 metal Substances 0.000 claims description 34
- 239000002738 chelating agent Substances 0.000 claims description 33
- 239000006166 lysate Substances 0.000 claims description 32
- 238000000746 purification Methods 0.000 claims description 30
- 102000006382 Ribonucleases Human genes 0.000 claims description 29
- 108010083644 Ribonucleases Proteins 0.000 claims description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- 238000002955 isolation Methods 0.000 claims description 23
- 150000003839 salts Chemical class 0.000 claims description 23
- 238000004113 cell culture Methods 0.000 claims description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical group OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 19
- 229920004890 Triton X-100 Polymers 0.000 claims description 19
- 239000013504 Triton X-100 Substances 0.000 claims description 19
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 16
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 14
- 229920001223 polyethylene glycol Polymers 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 150000001298 alcohols Chemical class 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 11
- 239000011159 matrix material Substances 0.000 claims description 11
- -1 polyoxyethylene Polymers 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 10
- 210000005253 yeast cell Anatomy 0.000 claims description 10
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- 239000000523 sample Substances 0.000 claims description 9
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims description 9
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 8
- 239000002202 Polyethylene glycol Substances 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 6
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims description 6
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims description 6
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 claims description 5
- 102000005891 Pancreatic ribonuclease Human genes 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- FCKYPQBAHLOOJQ-UHFFFAOYSA-N Cyclohexane-1,2-diaminetetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UHFFFAOYSA-N 0.000 claims description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims description 4
- 108010056929 lyticase Proteins 0.000 claims description 4
- CGVLVOOFCGWBCS-RGDJUOJXSA-N n-octyl β-d-thioglucopyranoside Chemical group CCCCCCCCS[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O CGVLVOOFCGWBCS-RGDJUOJXSA-N 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 235000009518 sodium iodide Nutrition 0.000 claims description 3
- 229960000789 guanidine hydrochloride Drugs 0.000 claims description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 2
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 claims description 2
- 235000011056 potassium acetate Nutrition 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 claims description 2
- 229910001488 sodium perchlorate Inorganic materials 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 claims 2
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- 239000011833 salt mixture Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 description 139
- 239000013612 plasmid Substances 0.000 description 53
- 238000005119 centrifugation Methods 0.000 description 21
- 239000000306 component Substances 0.000 description 18
- 238000012163 sequencing technique Methods 0.000 description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 10
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 10
- 238000004090 dissolution Methods 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 8
- 239000011521 glass Substances 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000006137 Luria-Bertani broth Substances 0.000 description 6
- 238000003306 harvesting Methods 0.000 description 6
- 238000003828 vacuum filtration Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
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- 238000009472 formulation Methods 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 230000006037 cell lysis Effects 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 238000013492 plasmid preparation Methods 0.000 description 4
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- 210000003296 saliva Anatomy 0.000 description 4
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- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 230000003196 chaotropic effect Effects 0.000 description 3
- 239000011859 microparticle Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- 229920002873 Polyethylenimine Polymers 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
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- 238000002123 RNA extraction Methods 0.000 description 2
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 238000005571 anion exchange chromatography Methods 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
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- 239000011230 binding agent Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 229940079920 digestives acid preparations Drugs 0.000 description 2
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 108010093305 exopolygalacturonase Proteins 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
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- 239000013598 vector Substances 0.000 description 2
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- ODWNBAWYDSWOAF-UHFFFAOYSA-N 2,4,4-trimethylpentan-2-yloxybenzene Chemical compound CC(C)(C)CC(C)(C)OC1=CC=CC=C1 ODWNBAWYDSWOAF-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- JYCQQPHGFMYQCF-UHFFFAOYSA-N 4-tert-Octylphenol monoethoxylate Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1 JYCQQPHGFMYQCF-UHFFFAOYSA-N 0.000 description 1
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000013373 clone screening Methods 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
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- 239000003550 marker Substances 0.000 description 1
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- 238000010369 molecular cloning Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
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- 238000001821 nucleic acid purification Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
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- 229920003023 plastic Polymers 0.000 description 1
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- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53362403P | 2003-12-30 | 2003-12-30 | |
| US60/533,624 | 2003-12-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20060129340A true KR20060129340A (ko) | 2006-12-15 |
Family
ID=34794239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020067015339A Withdrawn KR20060129340A (ko) | 2003-12-30 | 2004-12-30 | 효소 소화에 의한 핵산의 신속한 제조 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20050164260A1 (https=) |
| EP (1) | EP1709197A4 (https=) |
| JP (1) | JP2007516729A (https=) |
| KR (1) | KR20060129340A (https=) |
| IL (1) | IL176609A0 (https=) |
| NO (1) | NO20063278L (https=) |
| WO (1) | WO2005068662A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025063721A1 (ko) * | 2023-09-22 | 2025-03-27 | 주식회사 제노헬릭스 | 핵산 정제 또는 추출용 조성물 및 이의 용도 |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005057334A1 (de) * | 2005-11-28 | 2007-06-06 | Aj Innuscreen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien |
| EP2281826B1 (en) * | 2008-04-02 | 2016-02-17 | Rbc Bioscience Corp. | Reagents, chaotropic agents, kits and methods for isolating nucleic acids based on magnetic cellulose materials |
| BR112012004699B1 (pt) | 2009-09-03 | 2019-10-15 | Becton, Dickinson And Company | Métodos, composições e kits para lise química direta |
| WO2012044991A1 (en) | 2010-09-30 | 2012-04-05 | Phynexus, Inc. | Purification of nucleic acids |
| US10072284B2 (en) | 2012-06-21 | 2018-09-11 | Monsanto Technology Llc | Lysis buffer and methods for extraction of DNA from plant material |
| TWI555850B (zh) * | 2012-08-09 | 2016-11-01 | 財團法人工業技術研究院 | 用於分離核酸的組合物及方法 |
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2004
- 2004-12-30 WO PCT/US2004/043980 patent/WO2005068662A1/en not_active Ceased
- 2004-12-30 US US11/027,150 patent/US20050164260A1/en not_active Abandoned
- 2004-12-30 EP EP04815967A patent/EP1709197A4/en not_active Withdrawn
- 2004-12-30 JP JP2006547582A patent/JP2007516729A/ja not_active Withdrawn
- 2004-12-30 KR KR1020067015339A patent/KR20060129340A/ko not_active Withdrawn
-
2006
- 2006-06-28 IL IL176609A patent/IL176609A0/en unknown
- 2006-07-14 NO NO20063278A patent/NO20063278L/no unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025063721A1 (ko) * | 2023-09-22 | 2025-03-27 | 주식회사 제노헬릭스 | 핵산 정제 또는 추출용 조성물 및 이의 용도 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005068662A1 (en) | 2005-07-28 |
| NO20063278L (no) | 2006-09-29 |
| EP1709197A4 (en) | 2007-07-04 |
| IL176609A0 (en) | 2006-10-31 |
| JP2007516729A (ja) | 2007-06-28 |
| US20050164260A1 (en) | 2005-07-28 |
| EP1709197A1 (en) | 2006-10-11 |
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