JP2007516722A - マイクロ流体装置および沈殿試薬を使用した核酸を単離する方法およびキット - Google Patents

マイクロ流体装置および沈殿試薬を使用した核酸を単離する方法およびキット Download PDF

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Publication number
JP2007516722A
JP2007516722A JP2006546978A JP2006546978A JP2007516722A JP 2007516722 A JP2007516722 A JP 2007516722A JP 2006546978 A JP2006546978 A JP 2006546978A JP 2006546978 A JP2006546978 A JP 2006546978A JP 2007516722 A JP2007516722 A JP 2007516722A
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sample
nucleic acid
chamber
processing chamber
valved
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JP2007516722A5 (enExample
Inventor
ブイ． パーシャサラシー，ランジャニ
ケー． エリクソン，カティア
ベディンガム，ウィリアム
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3M Innovative Properties Co
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3M Innovative Properties Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2006546978A 2003-12-24 2004-10-25 マイクロ流体装置および沈殿試薬を使用した核酸を単離する方法およびキット Withdrawn JP2007516722A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53252303P 2003-12-24 2003-12-24
US10/852,022 US20050142570A1 (en) 2003-12-24 2004-05-24 Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
PCT/US2004/035330 WO2005068626A1 (en) 2003-12-24 2004-10-25 Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent

Publications (2)

Publication Number Publication Date
JP2007516722A true JP2007516722A (ja) 2007-06-28
JP2007516722A5 JP2007516722A5 (enExample) 2007-11-22

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JP2006546978A Withdrawn JP2007516722A (ja) 2003-12-24 2004-10-25 マイクロ流体装置および沈殿試薬を使用した核酸を単離する方法およびキット

Country Status (6)

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US (1) US20050142570A1 (enExample)
EP (1) EP1697512A1 (enExample)
JP (1) JP2007516722A (enExample)
AU (1) AU2004313912A1 (enExample)
CA (1) CA2551156A1 (enExample)
WO (1) WO2005068626A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008072904A (ja) * 2006-09-19 2008-04-03 Eiken Chem Co Ltd 核酸増幅反応における阻害を回避する方法
JP2011528552A (ja) * 2008-07-18 2011-11-24 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド 微小流体ｄｎａ試料調製のための方法およびシステム
JP2012533296A (ja) * 2009-07-17 2012-12-27 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド マイクロ流体装置においてｄｎａを単離する方法及びシステム
JP2014501517A (ja) * 2010-11-30 2014-01-23 クワンタムディーエックス・グループ・リミテッド 核酸抽出及び分画のためのマイクロ流体デバイス
US9116088B2 (en) 2009-07-17 2015-08-25 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
WO2024203442A1 (ja) * 2023-03-27 2024-10-03 京セラ株式会社 遺伝子検査装置

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US7322254B2 (en) * 2003-12-12 2008-01-29 3M Innovative Properties Company Variable valve apparatus and methods
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7387877B2 (en) * 2004-04-06 2008-06-17 Oro Grande Technology Bio-sensor and bio-sensor reporting system
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
US20080121591A1 (en) * 2006-11-29 2008-05-29 Canon U.S. Life Sciences, Inc. Device for nucleic acid preparation
US20090130746A1 (en) * 2007-10-25 2009-05-21 Canon U.S. Life Sciences, Inc. Microchannel surface coating
DE102008026058A1 (de) 2008-05-30 2009-12-03 Qiagen Gmbh Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren
JP2013507943A (ja) * 2009-10-21 2013-03-07 リボックス・ゲーエムベーハー Ｒｎａの指数関数的増幅のための方法およびｒｎａリアクター
USD667561S1 (en) 2009-11-13 2012-09-18 3M Innovative Properties Company Sample processing disk cover
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
USD638550S1 (en) 2009-11-13 2011-05-24 3M Innovative Properties Company Sample processing disk cover
USD638951S1 (en) 2009-11-13 2011-05-31 3M Innovative Properties Company Sample processing disk cover
WO2012158997A1 (en) 2011-05-18 2012-11-22 3M Innovative Properties Company Systems and methods for detecting the presence of a selected volume of material in a sample processing device
USD672467S1 (en) 2011-05-18 2012-12-11 3M Innovative Properties Company Rotatable sample processing disk
CN103501908B (zh) 2011-05-18 2016-03-16 3M创新有限公司 用于样品处理装置上阀调的系统和方法
BR112013027903B1 (pt) 2011-05-18 2021-01-12 Diasorin S.P.A. estrutura de medição em um dispositivo de processamento de amostras e método para a medição volumétrica de referido dispositivo
US8852919B2 (en) 2011-11-17 2014-10-07 Rheonix, Inc. Microfluidic apparatus, method, and applications
CN104762200B (zh) * 2015-04-08 2016-06-08 冯晓均 一种核酸检测反应管及其使用方法
US10859563B2 (en) 2015-12-01 2020-12-08 General Electric Company Erythrocyte aggregation and leukocyte isolation
JP7277050B2 (ja) 2016-01-29 2023-05-18 ピューリジェン バイオシステムズ， インコーポレイテッド 核酸の精製のための等速電気泳動
CN109414695B (zh) * 2016-03-14 2022-03-18 海利克斯拜恩德股份有限公司 集成流体装置及相关方法
CA3053576A1 (en) * 2017-02-27 2018-08-30 miDiagnostics NV System and method for purifying and amplifying nucleic acids
AU2018312570B2 (en) 2017-08-02 2024-01-11 Purigen Biosystems, Inc. Systems, devices, and methods for isotachophoresis
US20210237064A1 (en) 2020-01-31 2021-08-05 Astrin Biosciences, Inc. Biological Fluid Filtration System
CN114076823B (zh) * 2020-08-13 2024-05-17 深圳迈瑞生物医疗电子股份有限公司 固相组分的制备方法及所制备的固相组分

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3157635A (en) * 1960-08-05 1964-11-17 Takeda Chemical Industries Ltd Sulfonic acid cation exchange resin purification of nucleotides
US4399009A (en) * 1981-01-19 1983-08-16 Oronzio Denora Impianti Elettrochimici S.P.A. Electrolytic cell and method
US4839296A (en) * 1985-10-18 1989-06-13 Chem-Elec, Inc. Blood plasma test method
US5079155A (en) * 1987-03-02 1992-01-07 E. I. Du Pont De Nemours And Company Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
US6093558A (en) * 1991-07-25 2000-07-25 Edge Biosystems, Inc. Binding protein of biologically active compositions to an adhesive formulation on a substrate
DE69316231T2 (de) * 1992-10-21 1998-08-20 Cornell Res Foundation Inc Selektive, die porengrösse betreffende chemische modifikation poröser materialien
US6780818B2 (en) * 1994-02-02 2004-08-24 The Regents Of The University Of California Quantitative organic vapor-particle sampler
SE9500183D0 (sv) * 1995-01-20 1995-01-20 Pharmacia Biotech Ab Method for the purifivation of short nucleic acids
US5705059A (en) * 1995-02-27 1998-01-06 Miltenyi; Stefan Magnetic separation apparatus
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5620869A (en) * 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
US6068751A (en) * 1995-12-18 2000-05-30 Neukermans; Armand P. Microfluidic valve and integrated microfluidic system
JP3818689B2 (ja) * 1996-01-16 2006-09-06 富士写真フイルム株式会社 コロイド状シリカをコアとし、有機ポリマーをシェルとするコア／シェル状複合粒子の水性分散物及びその製造方法
US5837203A (en) * 1996-04-09 1998-11-17 Sievers Instruments, Inc. Device to alternately supply a fluid to an analyzer
US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US6143248A (en) * 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
WO1998028623A1 (en) * 1996-12-20 1998-07-02 Gamera Bioscience Corporation An affinity binding-based system for detecting particulates in a fluid
US6063589A (en) * 1997-05-23 2000-05-16 Gamera Bioscience Corporation Devices and methods for using centripetal acceleration to drive fluid movement on a microfluidics system
US6241980B1 (en) * 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
EP1179585B1 (en) * 1997-12-24 2008-07-09 Cepheid Device and method for lysis
EP1092144A1 (de) * 1998-06-29 2001-04-18 Evotec BioSystems AG Verfahren und vorrichtung zur manipulation von partikeln in mikrosystemen
JP3892594B2 (ja) * 1998-10-07 2007-03-14 株式会社東芝 超音波診断装置
US6240790B1 (en) * 1998-11-09 2001-06-05 Agilent Technologies, Inc. Device for high throughout sample processing, analysis and collection, and methods of use thereof
EP1230544B1 (en) * 1999-06-18 2004-07-28 Gamera Bioscience Corporation Devices and methods for the performance of miniaturized homogeneous assays
WO2001030873A1 (en) * 1999-10-27 2001-05-03 3M Innovative Properties Company Fluorochemical sulfonamide surfactants
AU3986501A (en) * 2000-02-23 2001-09-03 Zyomyx Inc Chips having elevated sample surfaces
JP2003533681A (ja) * 2000-05-15 2003-11-11 テカン・トレーディング・アクチェンゲゼルシャフト ミクロ流体装置および細胞ベースの分析を実行する方法
US6734401B2 (en) * 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6627159B1 (en) * 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6504021B2 (en) * 2000-07-05 2003-01-07 Edge Biosystems, Inc. Ion exchange method for DNA purification
US6537502B1 (en) * 2000-07-25 2003-03-25 Harvard Apparatus, Inc. Surface coated housing for sample preparation
US6919058B2 (en) * 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
US7189368B2 (en) * 2001-09-17 2007-03-13 Gyros Patent Ab Functional unit enabling controlled flow in a microfluidic device
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US6532997B1 (en) * 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
US7981600B2 (en) * 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US7322254B2 (en) * 2003-12-12 2008-01-29 3M Innovative Properties Company Variable valve apparatus and methods
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7939249B2 (en) * 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) * 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008072904A (ja) * 2006-09-19 2008-04-03 Eiken Chem Co Ltd 核酸増幅反応における阻害を回避する方法
JP2011528552A (ja) * 2008-07-18 2011-11-24 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド 微小流体ｄｎａ試料調製のための方法およびシステム
US9513196B2 (en) 2008-07-18 2016-12-06 Canon U.S. Life Sciences, Inc. Methods and systems for microfluidic DNA sample preparation
JP2012533296A (ja) * 2009-07-17 2012-12-27 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド マイクロ流体装置においてｄｎａを単離する方法及びシステム
US9116088B2 (en) 2009-07-17 2015-08-25 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
US9938519B2 (en) 2009-07-17 2018-04-10 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
JP2014501517A (ja) * 2010-11-30 2014-01-23 クワンタムディーエックス・グループ・リミテッド 核酸抽出及び分画のためのマイクロ流体デバイス
JP2017079766A (ja) * 2010-11-30 2017-05-18 クワンタムディーエックス・グループ・リミテッド 核酸抽出及び分画のためのマイクロ流体デバイス
WO2024203442A1 (ja) * 2023-03-27 2024-10-03 京セラ株式会社 遺伝子検査装置

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EP1697512A1 (en) 2006-09-06
US20050142570A1 (en) 2005-06-30
CA2551156A1 (en) 2005-07-28
WO2005068626A1 (en) 2005-07-28
AU2004313912A1 (en) 2005-07-28

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