JP2007277153A - Preservative for cosmetic or quasi-drug or food and drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a preservative useful as a cosmetic or a quasi-drug and food and drink. <P>SOLUTION: Apolactoferrin exhibits an antibacterial activity and is an environmentally friendly substance safe for human bodies and useful as the preservative for the cosmetic or quasi-drug or food and drink. The cosmetic or the quasi-drug, the food, a soft drink or an alcoholic beverage comprises the apolactoferrin as the preservative. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a preservative useful for cosmetics or quasi drugs and foods and drinks.

  Conventionally, as an ingredient having antiseptic function in cosmetics or quasi-drugs and foods and drinks, organic acids such as benzoic acid and dehydroacetic acid; alcohols such as phenylethanol and phenoxyisopropyl alcohol; hydroxybenzoic acid esters and chlorcresol 1 type or 2 types or more, such as chlorhexidine and propylene glycol which are not classified into these, or quaternary ammonium salts, such as benzalkonium chloride and benzethonium chloride; However, at present, the safety of these components has become a social problem.

  Natural fragrances exhibiting antibacterial properties such as rose oil, geranium oil, lavender oil and lemon oil are also attracting attention as ingredients having antiseptic functions. However, it is difficult to impart an antiseptic function with only these natural fragrances as the fragrance rate of the product decreases.

  Ingredients that are easy for microorganisms to propagate (for example, glycerin, amino acids, hyaluronic acid, collagen, placenta extract, gum, vitamins, etc.) and ingredients derived from animals and plants (for example, fats and oils, waxes, surfactants, etc.) There are also methods for producing cosmetics or quasi drugs or foods and drinks. However, cosmetics, quasi-drugs or foods and drinks manufactured in this way may still cause spoilage and the functionality expected of the product may be reduced.

  Lactoferrin is present in body fluids of many mammals, such as milk. In particular, it is known that colostrum of breast milk contains 5 to 10 g / L and occupies 30% to 70% of the total protein contained. Lactoferrin is an important protein for maintaining and developing the health of infants, and has recently been found to have antibacterial and antibacterial effects, and is used in various fields in addition to the food industry.

  Lactoferrin is an iron-binding glycoprotein having a molecular weight of about 80,000 in which two irons are bound in one molecule, and iron is released under acidic conditions such as pH 2 to become apolactoferrin. Regarding the bacteriostatic (antibacterial) action of lactoferrin, it is considered as follows. Due to the chelating action of apolactoferrin, the iron necessary for the growth of microorganisms is deprived and its growth is restricted. For this reason, microorganisms that strongly require iron during growth receive the bacteriostatic (antibacterial) action of lactoferrin. Such a bacteriostatic (antibacterial) action of lactoferrin has been considered particularly in the intestinal environment. Thus, the chelating action of apolactoferrin is noted with respect to the bacteriostatic (antibacterial) action of lactoferrin.

Lactoferrin is also known to be present in large amounts in human tears. An eye drop and eye ointment for treating eye diseases containing lactoferrin as a preservative is described in Patent Document 1. Patent Document 2 describes that an ophthalmic preparation for treating glaucoma and intraocular pressure contains an antimicrobial peptide such as lactoferrin as a preservative.
JP-A-9-30966 JP 2005-526092 A

  An object of the present invention is to provide a preservative useful for cosmetics or quasi drugs and foods and drinks.

  The present invention provides a preservative for cosmetics or quasi drugs comprising apolactoferrin.

  Furthermore, the present invention provides a cosmetic or quasi drug containing the preservative for cosmetics or quasi drugs.

  In one embodiment, the cosmetic or quasi drug has a preservative concentration of 0.05 mass% to 10.0 mass%.

  In another embodiment, the cosmetic or quasi drug has a preservative concentration of 0.5 to 1.0 mass%.

  The present invention also provides a food and beverage preservative comprising apolactoferrin.

  Furthermore, this invention provides the foodstuff containing the said food-drinks antiseptic | preservative.

  In one embodiment, the food has a preservative concentration of 0.05% to 10.0% by mass.

  Furthermore, this invention provides the soft drink containing the said foodstuff preservative.

  In one embodiment, the soft drink has a preservative concentration of 0.05% by mass to 5.0% by mass.

  Furthermore, this invention provides the alcoholic beverage containing the said food-drinks antiseptic | preservative.

  In one embodiment, the alcoholic beverage has a preservative concentration of 0.01% by mass to 5.0% by mass.

  According to the present invention, apolactoferrin can be used as a preservative in cosmetics or quasi drugs or food and drink. Cosmetics or quasi-drugs or foods and drinks containing apolactoferrin have fewer safety issues than products containing conventional preservatives.

(Preparation of apolactoferrin)
The preservative of this invention consists of apolactoferrin. The origin of the apolactoferrin used in the present invention is not particularly limited.

  Apolactoferrin usually dissociates divalent metal ions (for example, iron ions) of lactoferrin molecules by adjusting the pH of the aqueous solution containing lactoferrin to the acidic side (for example, 0.5 to 3.0). Can be manufactured. Apolactoferrin has a metal ion (for example, iron ion) bond degree in the molecule of 5% or less, preferably 3% or less. The degree of binding of metal ions can be determined by measuring the absorbance of iron-bound lactoferrin by spectroscopic analysis, or by directly measuring the iron content in lactoferrin by atomic absorption analysis or ICP spectroscopic analysis.

  Lactoferrin, which is a raw material for the production of apolactoferrin, is separated and purified from mammalian secretions such as milk (eg, milk) or processed milk products such as skim milk and whey (eg, whey). Can be obtained by using a method such as desorption with a high-concentration salt solution, separation method by electrophoresis, separation method by affinity chromatography, etc. It may be produced by cells (including microorganisms, plant cells, animal cells, insect cells, etc.), plants, animals and the like. Lactoferrin may be commercially available as pharmaceuticals, reagents, and the like. Lactoferrin is preferably derived from natural products. Preferably, it is derived from whey. Whey obtained as a by-product generated when producing a dairy product (for example, cheese, casein, etc.) from cow milk or skim milk can be suitably used as a source of lactoferrin.

  As a pH adjuster of the aqueous solution containing lactoferrin, for example, hydrochloric acid, phosphoric acid, phthalic acid, glycine and the like can be used. In order to produce apolactoferrin, the pH adjuster is added to an aqueous solution containing lactoferrin in an amount that adjusts the pH to the above value.

  Apolactoferrin is, for example, an acid (for example, hydrochloric acid, phosphoric acid, nitric acid, malic acid, or citric acid) added during ultrafiltration of lactoferrin, and metal ions (for example, iron ions) bound to lactoferrin. It can be suitably produced by dissociating. Alternatively, for example, apolactoferrin is partitioned by a bipolar membrane and a cation exchange membrane, which are composite ion exchange membranes having a structure in which a cation exchange membrane and an anion exchange membrane are bonded to each other. It can be suitably manufactured also by using an electrodialyzer having an acid chamber and a base chamber. The production of apolactoferrin is described in detail in Example 1 below, but the production method of apolactoferrin is not limited to this.

  When producing apolactoferrin from lactoferrin, apolactoferrin can be obtained in the form of an aqueous solution. When apolactoferrin is used as a preservative, it may be used in the form of an aqueous solution or in a powdered form after removing the solvent.

  Apolactoferrin may be commercially available as a pharmaceutical product. However, for the purpose of the present invention, it is preferable to use a commercially available product which does not contain other components and is produced so as to contain apolactoferrin alone. What was refine | purified to the purity normally used for medical use can be used suitably.

(Cosmetics or quasi drugs containing apolactoferrin)
Apolactoferrin can be contained as a preservative in cosmetics or quasi drugs. Apolactoferrin can exhibit good antibacterial action in, for example, lotions, as shown in the following examples.

  The term “cosmetics” as used in this specification refers to an object that is applied to the body by rubbing, spraying, etc. in order to keep the skin or hair healthy or to clean the body, beautify it, increase its appeal and change its appearance. Say. Cosmetics are classified into basic cosmetics, cosmetics for makeup, cosmetics for hair, and the like according to their uses. In the present specification, “cosmetics” includes those classified as quasi-drugs as defined in the Pharmaceutical Affairs Law, such as medicinal cosmetics. Cosmetics or quasi-drugs are, for example, cold creams, burnishing creams, mixed creams that are oily, massage creams, emollient creams, hizenic creams, eye and finger creams, lip balms, shaving creams Creams such as after-shave cream; moisturizing lotions, astringent lotions, acidic lotions, alkaline lotions, carmine lotions, after-shave lotions, etc .; emulsions such as emulsions containing polymer systems; Beauty oils; Body scrubs; Packs and peelable packs; foundation creams, burnishing type foundations, fountains, water fountains, kneading trousers, oily foundations including sticks, oil-in-water or water-in-oil emulsions Yup cream foundation, powder foundation, liquid foundation, lipstick, blusher, brushing powder, eyeliner, eyeshadow, mascara, eyebrow, functional lipstick, lip liner pencil, lip gloss, lip balm, nail enamel, base coat, top coat, Make-up products including light removal liquid, nail cream, cuticle remover, etc .; shampoo, conditioning shampoo, rinse, hair treatment, rinse integrated shampoo, hair restorer, hair nourishing agent, hair mousse and hair foam, hair spray, hair mist, hair gel, set Lotion, hair liquid, scalp treatment, hair cream, hair oil, hair coating lotion, hair gloss spray, hair Hair products including waxes, pomades, tics, hair waxes, hair dyes, hair bleaches, etc .; and bath and facial soaps, body cleansers, baths, bubble baths, deodorants, antiperspirants, slimming, Body care products including, but not limited to, fragrance, perfume, perfume, eau de toilette, eau de cologne and the like.

  In addition to apolactoferrin, ingredients that can be included in the cosmetic or quasi-drug of the present invention include the following: oils, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, silicones Oil-based raw materials including purified water, alcohol-containing aqueous raw materials; natural moisturizing factors including polyhydric alcohols, sugars, biopolymers, sodium pyrrolidonecarboxylate, amino acids, urea, sodium lactate; animal and plant extracts Natural surfactants including lactic acid bacteria culture, casein, yeast extract, royal jelly, silk extract, seaweed extract, sodium alginate, etc .; semi-synthetic surfactants including carboxymethylcellulose, etc .; methacrylic acid copolymer, etc. Synthetic surfactants including: carboxylates, sulfonates, phosphate esters, quaternary Hydrophilic group fluorosurfactants including ammonium salts, trimethylglycine, amine oxide, polyoxyethylene, etc .; nonionic systems including polyether modification, anionic systems including sulfonic acid modification, cationic systems including ammonium modification , Amphoteric silicone surfactants including modified sulfobetaines; polypeptide derivative surfactants including surfactin and lipopeptides; natural animal and plant surfactants such as glycolipids, phospholipids and bile acids ; Microbial production-type natural surfactants including sophorolipids; Powder raw materials for shaping and spreading; UV protection, stratum corneum peeling and dissolution, soothing, deodorizing, nutritional supplementation, antiperspirant, whitening, cell activation Raw materials for pharmaceuticals for the purpose of promoting blood circulation, anti-inflammation, astringency, sebum; antiseborrheic agent;

  Apolactoferrin, which is the preservative of the present invention, can be added, blended or contained in cosmetics or quasi-drugs so as not to impair the efficacy of the above ingredients. The content of apolactoferrin depends on the type and ingredients of the cosmetic or quasi-drug, but is preferably 0.05 to 10.0% by mass, more preferably 0.5 to 7.0% by mass, and still more preferably It may be 0.5 to 1.0% by mass. This content is not greatly different depending on the form or application (for foundation, for washing, etc.) such as cream, liquid, emulsion, mousse or solid.

  Apolactoferrin can be added to, blended with, or contained in cosmetics or quasi drugs by procedures commonly used by those skilled in the art. For example, after being pre-dissolved in a solvent such as water or alcohol, it is added to a cosmetic or quasi-drug by mixing with other ingredients or by stirring and mixing with other ingredients as a powder. Can be included.

  The cosmetic or quasi-drug of the present invention can be sealed by being placed in a container (for example, a bottle, an aluminum pouch, etc.) such as plastic or glass. Storage and transportation of the cosmetic or quasi drug of the present invention can be done in the same way as is normally done for the intended product. The cosmetic or quasi-drug of the present invention can be excellent in stability in daily use in addition to storage in an unused state. In particular, contamination with bacteria can be prevented under daily use.

(Food containing apolactoferrin, soft drink or alcoholic beverage)
Also in food and drink, the antibacterial action of apolactoferrin can be used. Apolactoferrin can be included as a preservative in foods, soft drinks, or alcoholic beverages.

  As used herein, “food” refers to all foods that are prepared for eating. In particular, foods that can be stored in daily life (for example, stored in an unopened state after purchase or stored after opening) are included. Specifically, pickles; canned foods; pouched foods; dried foods; heated foods; frozen foods; chilled foods; freeze-dried foods; breads, powdered cereals, ground cereals; Paste; raw food confectionery; pudding, margarine, butter, cheese, etc .; meat; seafood, paste products containing all or part of vegetables, ham, smoked food, seasoned food, cooked food; Agar; noodles; instant miso soup; foods for pets; foods for livestock; foods intended for nutritional supplements, but not limited thereto.

  The content of apolactoferrin that is the preservative of the present invention depends on the type and ingredients of the food, but is preferably 0.05 to 10.0% by mass, more preferably 0.5 to 2.0% by mass, and further Preferably it may be 0.5-1.0 mass%.

  Apolactoferrin can be added, blended, or contained, for example, in the process of manufacturing the types of foods described above. Since apolactoferrin is water-soluble, it can be added, blended or contained in foods in the form of powder or aqueous solution or dissolved in a solvent used for food production such as ethanol.

  As used herein, “soft drink” refers to soft drinks other than water (beverages containing less than 1% by volume of alcohol). Specific examples include carbonated drinks, fruit juice drinks, vegetable drinks, milk drinks, and coffee drinks, but are not limited thereto. Also included are those intended to be consumed as a drink by dilution, thawing, etc. (for example, concentrated juice, frozen juice, etc.). Usually, soft drinks do not contain milk.

  The content of apolactoferrin, which is the preservative of the present invention, depends on the type and components of the soft drink, but is preferably 0.05 to 5.0% by mass, more preferably 0.2 to 2.0% by mass. More preferably, it may be 0.5 to 1.0% by mass.

  Apolactoferrin can be added, blended, or contained, for example, in the process of producing a soft drink of the type described above. Since apolactoferrin is water-soluble, it can be added to, blended with, or contained in soft drinks in the form of a powder or an aqueous solution or dissolved in a solvent used for producing soft drinks such as ethanol.

  The “alcoholic beverage” in the present specification is a beverage containing ethyl alcohol (ethanol) and a beverage containing an alcohol content of 1% by volume or more. Specific examples include, but are not limited to, happoshu, distilled liquor, brewed liquor, beer and liqueur. Examples of the raw materials for sake include, but are not limited to, fruits such as grapes, apples, cherries and palm fruits; grains such as rice, wheat and corn; root vegetables such as potatoes and sweet potatoes; and sugarcane. Specific types of liquor include wine, brandy, cider (apple wine), calvados, refined sake, shochu (eg, rice shochu, shochu shochu), Shaoxing liquor, awamori, beer, malt whiskey, bourbon whiskey, grappa, marl , Ram, tequila (mescal), gin, vodka and the like.

  The content of apolactoferrin that is the preservative of the present invention depends on the type and components of the alcoholic beverage, but is preferably 0.01 to 5.0% by mass, more preferably 0.2 to 2.0% by mass, More preferably, it may be 0.5-1.0 mass%.

  Apolactoferrin can be added, blended, or contained, for example, in the process of manufacturing the type of alcoholic beverage described above. Since apolactoferrin is water-soluble, it can be added to, blended with, or contained in an alcoholic beverage in the form of a powder or an aqueous solution, or dissolved in a solvent used for producing an alcoholic beverage such as ethanol.

  When the preservative of the present invention is added to, blended with, or contained in foods, soft drinks, or alcoholic beverages, components that are blended in ordinary foods, soft drinks, or alcoholic beverages can be arbitrarily blended as appropriate. Ingredients include animal oil (milk fat, beef tallow, etc.), vegetable oil (olive oil, cacao oil, sesame oil, soybean oil, corn oil, cottonseed oil, etc.), sweetener (sucrose, fructose, glucose, palatinose, fructooligosaccharide, dextrin) , Aspartame, sugar alcohol, etc.), starch, thickener, gelling agent, paste, dietary fiber, umami seasoning, vitamins, salt, vinegar, soy sauce, spices, enzymes, fragrances, coloring agents, etc. However, it is not limited to these.

  The food products, soft drinks, or alcoholic beverages of the present invention can be easily put in as is usually done with the intended product. For example, it can be packaged (preferably sealed), can be sealed in a can, or can be sealed in a bottle of plastic, glass or the like. Storage and transport of the food products, soft drinks, or alcoholic beverages of the present invention can be done in the same way as is normally done with the intended product.

  EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, this invention is not limited to these illustrations.

(Example 1: Preparation of apolactoferrin)
As described below, apolactoferrin was prepared by adding an acid when ultrafiltrating lactoferrin and separating it with an acid added with iron bound to lactoferrin. As ultrafiltration equipment, ACP-0013 (hollow fiber module: membrane inner diameter 0.8mm, effective as a UF module manufactured by Asahi Kasei Chemicals Co., Ltd.) A membrane area of 170 cm ² , membrane material: polyacrylonitrile, nominal molecular weight cut-off: 13,000) was incorporated and used for the experiment.

  100 mL / mL lactoferrin (Wako Pure Chemical Industries, Ltd., special grade; containing about 30% iron) 1000 mL of an aqueous solution at room temperature, the operation starting pressure is set to 50 KPa at the module outlet pressure, and ultrafiltration is performed, and the volume becomes 500 mL. Concentrated and reduced to volume. Next, 0.05 mol / L of citric acid aqueous solution was added to the ultrafiltered solution until the volume reached 1000 mL, ultrafiltered, and concentrated and reduced until the volume reached 500 mL. The same operation was repeated again. Subsequently, 0.05 mol / L aqueous citric acid solution was added to the ultrafiltered solution until the volume reached 1000 mL, ultrafiltered, and concentrated and reduced until the volume reached 250 mL. The obtained concentrated solution was collected, and the amount of iron bound to lactoferrin was measured at an absorbance of 470 nm with an ICP spectrometer (SPS1200AR; manufactured by Seiko Instruments Inc.). It was confirmed that the amount of iron bound to lactoferrin was 3% or less, and this concentrated solution was used as an apolactoferrin aqueous solution. Further, the solvent was removed by an evaporator to obtain an apolactoferrin powder.

(Example 2: Production of lotion)
The manufacture example of the lotion containing apolactoferrin is shown.

  Water, apolactoferrin powder (prepared in Example 1), grape fermentation precipitate, ethanol, sodium pyrosulfite, glucosyl hesperidin, and sodium hyaluronate were mixed and dissolved at 40 to 60 ° C. to obtain A phase. On the other hand, glycerin, propylene glycol, glyceres-25 of PCA isostearate, and propylene glycol alginate were mixed at 40 to 60 ° C. so as to be a B phase. Next, A phase and B phase were mixed and stirred at room temperature to obtain a skin lotion.

  Table 1 below shows the composition of the lotion and the blending ratio (mass%).

(Comparative Example 1: Production of lotion)
A lotion was produced in the same manner as in Example 2 except that the apolactoferrin powder was replaced with lactoferrin powder (Wako Pure Chemical Industries, Ltd., special grade; containing about 30% iron).

(Example 3: Antibacterial test of lotion)
In order to examine the antibacterial activity of apolactoferrin in the lotion, the following two tests were conducted.

(3-1: Antibacterial test by microplate method)
In this test, it was investigated whether apolactoferrin in the lotion had antibacterial activity against bacteria with a high concentration of 10 ⁵ CFU / mL.

First, in examining the effect on high-concentration bacteria, the lotion produced in Example 2 (apolactoferrin-containing lotion) or the lotion produced in Comparative Example 1 (lactoferrin-containing lotion) was used with apolactoferrin or lactoferrin. Apo-lactoferrin powder or lactoferrin powder prepared in Example 1 (Wako Pure Chemical Industries, Ltd., special grade; about 30% iron) so that the concentration is 8% by mass, 4% by mass, 2% by mass, or 1% by mass Contained) and dissolved to obtain each lotion sample. Each 50 μL of these lotion samples was placed in a well of a 96-well plate, and then 100 μL of SCD broth medium (Nissui) and 50 μL of 10 ⁵ CFU / mL bacteria were added to each well. The bacteria used were Staphylococcus epidermidis, Listeria innocua, Bacillus cereus, and Escherichia coli. As a control for bacterial growth, apolactoferrin or a lotion containing no lactoferrin was used (apolactoferrin or 0% by mass lotion sample of lactoferrin). The cells were cultured at 35 ° C. for 24 hours, and the growth of the bacteria in the wells was measured for each bacterium by measuring the absorbance at 595 nm or coloring test with Alamar Blue. In these measurement results, since the lotion sample itself was colored, the evaluation was performed by subtracting the value of the well containing water instead of bacteria.

  The results are shown in FIG. In FIG. 1, the upper left graph shows the test result of Staphylococcus epidermidis, the upper right graph shows the test result of Listeria innocua, the lower left graph shows the test result of Bacillus cereus, and the lower right graph shows The test result of Escherichia coli is shown. The black circle in each graph shows the measurement result of apolactoferrin, and the white circle shows the measurement result of lactoferrin. The horizontal axis represents the concentration (mass%) of apolactoferrin or lactoferrin in the lotion, and the vertical axis represents the absorbance value. This absorbance value is a value obtained by subtracting the absorbance of the lotion sample well containing no bacteria from the absorbance of the lotion sample well to which bacteria and a medium are added. A lower absorbance value indicates fewer bacteria. In each graph of FIG. 1, the point represented by each symbol indicates the average value of the measured values of the three wells. In any bacterium, in the case of lactoferrin, the absorbance did not decrease significantly even when the concentration of lactoferrin in the lotion increased. On the other hand, in the case of apolactoferrin, the absorbance value decreased as the apolactoferrin concentration in the lotion increased. From this, it was clarified that apolactoferrin, unlike lactoferrin, causes a decrease in the number of bacteria in a concentration-dependent manner.

  When 8% by mass of apolactoferrin was contained in the lotion, the absorbance value was almost 0 in any bacteria. It was shown that a lotion sample containing 8% by weight of apolactoferrin can almost completely inhibit the growth of such high concentrations of bacteria.

  Even when 1% by mass of apolactoferrin was contained, the absorbance value was lowered. Therefore, it can be seen that the antibacterial action was observed by containing apolactoferrin in the lotion even at a concentration of 1% by mass.

(3-2: Antibacterial test by disk diffusion method)
Also in this antibacterial test, the 8 mass% apolactoferrin-containing lotion similar to the above 3-1 was used as a lotion sample.

In this test, Listeria innocua was used, and the cell concentration was adjusted to 10 ⁶ CFU / mL. As a test disk (filter paper), ADVANTEC No. One filter paper (manufactured by ADVANTEC) was cut into a circle of about 8 mm and used by autoclaving. Bacteria were inoculated into a petri dish containing tryptosa agar medium. For bacterial inoculation, the bacterial solution was uniformly streaked and smeared to cover the entire surface of the agar medium in the petri dish. About 2 drops of the above-mentioned lotion sample was dropped and soaked in the disc, and dried. The disc was placed on the medium and cultured at 35 ° C. for 40 hours. After incubation, the formation of a blocking circle on the disc was observed with the naked eye.

  After 40 hours of culturing, a complete circle of inhibition was seen around the disc. Thus, it was shown that 8 mass% apolactoferrin lotion can almost completely suppress the growth and growth of bacteria.

  From the above-mentioned two antibacterial tests, it can be seen that 8% by mass of apolactoferrin-containing lotion can almost completely suppress the growth of bacteria at a high concentration. However, in these antibacterial tests, such a high concentration of bacteria was used to facilitate the measurement, but under such daily use, such a high concentration of bacteria would not be mixed into the lotion. Conceivable. Rather, these antibacterial tests demonstrate that the inclusion of apolactoferrin in the lotion can inhibit the growth and growth of bacteria that can be mixed into the lotion.

(Example 4: Production of cosmetic cream)
The manufacture example of the cosmetic cream containing apolactoferrin is shown.

  White petrolatum, jojoba oil, behenyl alcohol, polyglyceryl-10 pentastearate, sodium stearoyl lactate, tocopherol, stearyl alcohol, squalane, glyceryl monostearate, polyoxyethylene hydrogenated castor oil 60, and ascorbyl dipalmitate at 60-80 ° C. It melt | dissolved and was set as the A phase. On the other hand, water, propylene glycol, placenta extract, ethanol, sodium hyaluronate, glycerin, and apolactoferrin powder (prepared in Example 1) are mixed and dissolved at 60 to 80 ° C., and xanthan gum is added. Thereto, sodium pyrosulfite, EDTA-2Na, and glucosyl hesperidin were added and dissolved uniformly to obtain a B phase. Next, A phase and B phase were emulsified by mixing at 50 degreeC, and the cream was obtained.

  Table 2 below shows the components and the mixing ratio (mass%) of the cosmetic cream.

(Example 5: Production of cosmetic emulsion)
The manufacture example of the cosmetic milk containing apolactoferrin is shown.

  Water, grape fermentation precipitate, apolactoferrin powder (prepared in Example 1), glucosyl hesperidin, and sodium hyaluronate were mixed and stirred at 50 to 60 ° C. to obtain A phase. On the other hand, squalane, lecithin, jojoba oil, and tocopherol were dissolved and mixed at 40 ° C. to obtain a B phase. Next, A phase and B phase were mixed and stirred and emulsified at 40 degreeC, and the emulsion was obtained.

  Table 3 below shows the blending components and blending ratio (mass%) of the cosmetic emulsion.

(Example 6: Production of pickles)
The manufacture example of the pickles containing apolactoferrin is shown.

  Slice the pepper and carrot thickly and immerse them in a solution of soy sauce and miso diluted with water. Next, apolactoferrin powder is added to 1% by mass with respect to 100 mL of the aqueous solution, mixed at room temperature, and allowed to stand for 3 to 5 hours to allow the solution to mix with pepper and carrots. This gives a pickled pepper and carrot.

(Example 7: Production of fruit juice beverage)
The manufacture example of the fruit juice drink containing apolactoferrin is shown.

  Squeeze tangerine and squeeze out the juice. Apolactoferrin powder is added to the above juice so as to be 1% by mass with respect to 100 mL of the juice and dissolved at room temperature to obtain a juice beverage.

(Example 8: Production of wine)
The example of manufacture of the wine containing apolactoferrin is shown.

  The grapes are squeezed to squeeze out the juice, and the juice is naturally fermented to obtain wine. Apolactoferrin powder is added to the wine so as to be 1% by mass with respect to 100 mL of the wine, and dissolved at room temperature to obtain wine.

  Apolactoferrin may exhibit antibacterial properties. Since apolactoferrin is a natural product, it is safe for the human body and environmentally friendly. This property of apolactoferrin is used as a preservative in cosmetics or quasi-drugs that may come in contact with bacteria due to the high frequency of daily use over a long period of several months and that directly touch the skin. Is preferable. Similarly, there is a possibility that various germs may be mixed especially during daily storage, and it is also preferable as a preservative in foods and drinks taken into the body.

It is a graph which shows the result of the antibacterial property test of the lotion sample containing various concentrations of apolactoferrin or lactoferrin against Staphylococcus epidermidis, Listeria innocua, Bacillus cereus, and Escherichia coli.

Claims (11)

  A preservative for cosmetics or quasi drugs made of apolactoferrin.   A cosmetic or quasi-drug containing the preservative according to claim 1.   The cosmetic or quasi drug according to claim 2, wherein the concentration of the preservative is 0.05 mass% to 10.0 mass%.   The cosmetic or quasi drug according to claim 3, wherein the concentration of the preservative is 0.5 to 1.0 mass%.   A preservative for food and drink comprising apolactoferrin.   A food containing the preservative according to claim 5.   The foodstuff of Claim 6 whose density | concentration of the said preservative is 0.05 mass%-10.0 mass%.   A soft drink containing the preservative according to claim 5.   The soft drink of Claim 8 whose density | concentration of the said preservative is 0.05 mass%-5.0 mass%.   An alcoholic beverage containing the preservative according to claim 5.   The alcoholic beverage of Claim 10 whose density | concentration of the said preservative is 0.01 mass%-5.0 mass%.

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