JP7029825B2 - Method for Producing Ceramide-Containing Composition - Google Patents

Description

The present invention relates to a method for producing a ceramide-containing composition, and more particularly to a method for producing a ceramide-containing composition in which an extraction operation is performed using an aqueous solvent containing no organic solvent.

Ceramides are a group of compounds constituting sphingolipids, and are a group of compounds having a ceramide skeleton in which sphingoids and fatty acids are amide-bonded. It is known that a large amount of a component consisting of a ceramide skeleton (free ceramide) is present in human keratin and contributes to adhesion between keratinocytes. Further, among ceramides, glucosylceramide in which one molecule of glucose is bound to the ceramide skeleton has been found to have a moisturizing effect and an anti-atopic effect, and is attracting attention as a material for cosmetics and pharmaceuticals.

Conventionally, ceramides have been extracted from the brain of cattle and the like, but since the occurrence of BSE, a safer source of supply has been required. For example, rice bran (Patent Document 1) and konjac (Patent Document 1). 2), a method for extracting ceramides from yeast (Patent Document 3) and the like has been proposed. In particular, yeast is capable of stable mass production and is attracting attention as a source of ceramides.

Japanese Unexamined Patent Publication No. 11-279586 Japanese Unexamined Patent Publication No. 2002-281936 Japanese Unexamined Patent Publication No. 2012-246287

By the way, when ceramides are extracted from plants and microorganisms, an organic solvent is used as an extraction solvent. However, the organic solvent may remain in the obtained extract, which may cause a problem in blending the extract containing ceramides into cosmetics and the like.

An object of the present invention is to provide a method for producing a ceramide-containing composition capable of efficiently extracting ceramides without using an organic solvent as an extraction solvent.

In order to solve the above problems, first, the present invention is a method for producing a ceramide-containing composition, which comprises extracting yeast by using an aqueous solvent containing a surfactant without containing an organic solvent. (Invention 1).

According to the present invention, since yeast is used as an extraction raw material and an aqueous solvent containing a surfactant without containing an organic solvent is used as an extraction solvent, ceramides can be efficiently produced without using an organic solvent. Can be extracted.

In the above invention (Invention 1), the aqueous solvent preferably contains 0.01 to 2% by mass of the surfactant (Invention 2).

In the above inventions (Inventions 1 and 2), it is preferable that the yeast is Saccharomyces veronae (Invention 3).

In the above inventions (Inventions 1 to 3), the surfactant is selected from the group consisting of a propylene glycol fatty acid ester, a glycerin fatty acid ester, a sucrose fatty acid ester, a sorbitan fatty acid ester, and a polyoxyethylene sorbitan fatty acid ester. Alternatively, it is preferable that the number is two or more (Invention 4).

Secondly, the present invention provides a ceramide-containing composition produced by the production method according to the above invention (Invention 1 to 4) (Invention 5).

Thirdly, the present invention provides a cosmetic comprising the ceramide-containing composition according to the above invention (Invention 5) (Invention 6).

Fourth, the present invention provides a food or drink characterized by blending a ceramide-containing composition produced by the production method according to the above invention (Invention 4) (Invention 7).

According to the present invention, ceramides can be efficiently extracted without using an organic solvent as the extraction solvent.

It is a photograph showing the result of thin layer chromatography (TLC). It is a photograph showing the result of thin layer chromatography (TLC).

Hereinafter, embodiments of the present invention will be described.
The method for producing a ceramide-containing composition according to the present embodiment is to extract yeast using an aqueous solvent containing a surfactant without containing an organic solvent.

Here, the "ceramides" in the present embodiment refer to compounds containing a ceramide skeleton in which sphingoids and fatty acids are amide-bonded, and may be referred to as "free ceramide" in the present specification. In addition to (.), The concept includes sugar ceramides in which the sphingoid moiety in the ceramide skeleton is glycoside-bonded with sugar (for example, celebrosides such as glucosylceramide and galactosylceramide).

1. 1. Extraction solvent The "aqueous solvent" in the present specification is used as a solvent for extracting ceramides from yeast, and refers to an aqueous solution containing water as a solvent, a surfactant described later, and if desired, a salt or the like. ..

The water that can be used in this embodiment includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Treatments applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmoregulation, buffering and the like. Therefore, the water that can be used as the aqueous solvent in the present embodiment includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

The aqueous solvent in this embodiment does not contain an organic solvent. Organic solvents (for example, chloroform, methanol, etc.) are widely used in the extraction of ceramides, but they may remain in the obtained ceramide-containing extract, and the extract may be added to cosmetics and the like. This is because safety concerns can arise.

In this embodiment, a surfactant is used in order to efficiently extract ceramides from yeast without using an organic solvent. Examples of the surfactant that can be used include a nonionic surfactant, an anionic surfactant, a cationic surfactant, and an amphoteric surfactant.

Specifically, examples of the nonionic surfactant that can be used in the present embodiment include propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, and POE (polyoxyethylene) sorbitan fatty acid. Esters, POE sorbit fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymers, POE / POP alkyl ethers , Polyether-modified silicone lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids and the like.

Examples of anionic surfactants include fatty acid sequens, α-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkylnaphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, and alkylamide sulfates. Alkyl phosphate, POE alkyl phosphate, alkyl amid phosphate, alkylyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolysis Examples thereof include collagen peptide salts and perfluoroalkyl phosphates.

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, disstearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and benza chloride. Examples thereof include luconium, behenic chloride amidopropyldimethylhydroxypropylammonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, and quaternary ammonium salts of lanolin derivatives.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imitazoline derivative type, amidoamine type and the like.

These surfactants can be used alone or in combination of two or more. Among these, nonionic surfactants are preferable because they have excellent extraction efficiency of fatty acids and are excellent in safety when the obtained composition containing fatty acids is blended in cosmetics and foods and drinks. A surfactant that can be used as an additive, such as propylene glycol fatty acid ester, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, or POE sorbitan fatty acid ester, is preferable, and POE sorbitan fatty acid ester is particularly preferable.

The aqueous solvent in the present embodiment preferably contains 0.01 to 2% by mass, particularly preferably 0.05 to 0.5% by mass, and 0.07 to 0.15% by mass of the above-mentioned surfactant. Is even more preferable. When the aqueous solvent contains a surfactant in the above range, ceramides can be efficiently extracted from yeast and impurities can be suppressed from being extracted.

In addition to the above-mentioned surfactant, additives such as salts and pH adjusters can be added to the aqueous solvent of the present embodiment as long as the effects of the present embodiment are not impaired.

2. 2. Extraction raw material The extraction raw material used in this embodiment is yeast. The yeast that can be used in the present embodiment is not particularly limited as long as it produces ceramides, but for example, the genus Saccharomyces such as Saccharomyces veronae and Saccharomyces kluyveri. The genus Schizosaccharomyces such as Schizosaccharomyces pombe; the genus Kluyveromyces thermotolerans, the genus Kluyveromyces lactis, etc. Zygosaccharomyces such as Zygosaccharomyces thermotolerans; Lacansea thermotolerans, Lacansea kluyveris, Lacansea yeast, Lacansea kluyveri, etc. Yeasts such as the genus Candida; are preferably exemplified. Saccharomyces cerevisiae (baker's yeast) does not produce ceramides and cannot be used in this embodiment.

In this embodiment, these yeasts can be used alone or in combination of two or more. Among these, the genus Saccharomyces (excluding Saccharomyces cerevisiae) is particularly preferable from the viewpoint of productivity of ceramides, availability and safety when used in cosmetics, and Saccharomyces veronae is further preferable. preferable.

In this embodiment, such yeast is cultivated. The culture type may be either batch culture or continuous culture. The medium used for culturing is not particularly limited as long as yeast can be cultivated, and contains a carbon source such as glucose; a nitrogen source such as an ammonium salt; an inorganic salt such as a magnesium salt; essential amino acids and vitamins. All you need is. Further, in addition to or in place of these, components commonly used in yeast media such as yeast extract, peptone, and casein may be used as the medium.

In the present embodiment, as the medium containing the above components, a medium commonly used for yeast culture can be preferably used, among which YPD medium, YM medium, ME medium and MRS medium are preferable, and MRS medium is particularly preferable. preferable.

Yeast is cultured by placing the above medium in a culture tank, inoculating yeast, and treating the yeast in a temperature range of 25 to 35 ° C, preferably 28 to 32 ° C for 12 to 72 hours, preferably 18 to 48 hours. It can be carried out.

In the culture solution at the end of the culture, the ethanol concentration is preferably 1% by volume or less, more preferably 0.5% by volume or less. Here, 1% by volume or less includes 0% by volume, that is, the case where the culture solution does not contain ethanol at all. Further, it is preferable that the above culture is mainly carried out by aerobic respiration and substantially no alcoholic fermentation is carried out. The yeast cells obtained by substantially not performing alcoholic fermentation have a low ethanol concentration, and therefore, the effect of the present embodiment when extracted with an aqueous solvent containing no organic solvent is exhibited.

The obtained culture broth may be subjected to an extraction operation by adding a surfactant as it is, but after collecting yeast cells by centrifugation, filtration, etc., an extraction operation is performed with an aqueous solvent containing a surfactant. It is preferable to do.

3. 3. Extraction method In this embodiment, ceramides are extracted using the yeast cells as the extraction raw material and the above-mentioned aqueous solvent as the extraction solvent.

In the extraction operation, the amount of the aqueous solvent containing the surfactant used is preferably 1.0 to 10 parts by mass, preferably 2.0 to 8.0, based on 100 parts by mass of the wet mass of the yeast cells. It is particularly preferably parts by mass, and even more preferably 3.0 to 6.0 parts by mass. The extraction time is preferably 1 to 10 hours, more preferably 1.5 to 5 hours. The extraction temperature is preferably 50 to 100 ° C, more preferably 70 to 90 ° C.

The ceramide-containing composition according to the present embodiment can be obtained by removing the extraction residue from the obtained extract by filtration, centrifugation or the like, and concentrating, freeze-drying or the like as desired.

The obtained ceramide-containing composition may be further purified, if necessary. The purification method is not particularly limited and can be carried out by a conventional method. For example, a method of recovering a fraction containing ceramides by subjecting to column chromatography using a porous substance such as silica gel or alumina, an ion exchange resin or the like can be mentioned.

Since the ceramide-containing composition thus obtained is extracted with an aqueous solvent that does not contain an organic solvent, it is highly safe, and in addition to the cosmetics and foods and drinks described later, compounding components such as pharmaceuticals and the like. Can be suitably used as.

4. Cosmetics The cosmetics according to the present embodiment contain the above-mentioned ceramide-containing composition. In the present embodiment, the ceramide-containing composition may be blended as it is, or a composition further formulated from the ceramide-containing composition may be blended. By blending the ceramide-containing composition with the cosmetic, it is possible to impart favorable effects of the ceramide, such as a moisturizing effect and an anti-atopic effect, to the cosmetic.

Here, the type of cosmetics to which the ceramide-containing composition can be blended is not particularly limited, and for example, in the case of skin cosmetics, ointments, creams, emulsions, lotions, packs, foundations and the like are exemplified. In the case of hair cosmetics, hair tonics, hair creams, hair liquids, shampoos, pomades, rinses and the like are exemplified.

The blending amount of the ceramide-containing composition can be appropriately adjusted according to the type of cosmetics, but the suitable blending ratio is about 0.0001 to 10% by mass in terms of the ceramide-containing content. A particularly suitable blending ratio is about 0.001 to 1% by mass.

The cosmetic of the present embodiment has a moisturizing effect, an anti-atopic effect, and a main agent, an auxiliary agent or other components used in the production of ordinary cosmetics, for example, an astringent agent and a bactericidal agent, as long as they do not interfere with the preferable effects of ceramides.・ Antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, antioxidant / active oxygen removers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters , A surfactant, a fragrance, etc. can be used in combination. Such concomitant use may result in a more general product and may result in better than normally expected synergistic effects with other concomitant active ingredients.

Since the above-mentioned cosmetics according to the present embodiment contain a ceramide-containing composition extracted with an aqueous solvent that does not contain an organic solvent, the ceramides have a favorable effect and are safe. Will be excellent.

5. Food and Beverage The food and drink according to the present embodiment contains a ceramide-containing composition extracted using a specific surfactant.

The ceramide-containing composition to be blended in the food or drink according to the present embodiment is selected from the group consisting of propylene glycol fatty acid ester, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, and polyoxyethylene sorbitan fatty acid ester. It is extracted using one kind or two or more kinds of surfactants. Since these specific surfactants can be used as food additives, ceramide-containing compositions extracted using such surfactants are particularly excellent in safety when blended in foods and drinks. It will be.

In the present embodiment, the ceramide-containing composition may be blended as it is, or a composition further formulated from the ceramide-containing composition may be blended. By blending the above-mentioned ceramide-containing composition into foods and drinks, it is possible to impart favorable effects of ceramides such as moisturizing action and anti-atopic action to foods and drinks.

Here, foods and drinks are those that are less likely to harm human health and are ingested by oral or gastrointestinal administration in normal social life, and are foods, pharmaceuticals, and quasi-drugs under administrative divisions. It is not limited to such categories. Therefore, the "food and drink" in the present embodiment includes general foods, health foods, functional foods, health functional foods (foods for specified health use, functional foods, nutritionally functional foods), and non-pharmaceutical foods that are orally ingested. It includes a wide range of products, pharmaceuticals, etc. The food and drink according to the present embodiment is preferably a food and drink capable of displaying the preferable action of ceramides on the food and drink or its packaging, and is a food with health claims (food for specified health use, food with functional claims). , Nutritionally functional foods), quasi-drugs and pharmaceuticals are particularly preferred.

When the above-mentioned ceramide-containing composition or the composition formulated from the above-mentioned ceramide-containing composition is blended in foods and drinks, the blending amount of the active ingredient in them is appropriately changed in consideration of the purpose of use, symptoms, sex, etc. However, in consideration of the general intake of foods and drinks to be added, it is preferable that the daily intake of ceramides for an adult is about 1 to 1000 mg. When the food or drink to be added is a granular, tablet-shaped or capsule-shaped food or drink, the amount of the ceramide-containing composition or the composition formulated from the ceramide-containing composition to be added is the food or drink to be added. On the other hand, it is usually 0.1 to 100% by mass, preferably 5 to 100% by mass in terms of the mass of ceramides.

The food or drink of the present embodiment may be a mixture of the above-mentioned ceramide-containing composition in any food or drink that does not interfere with its activity, or a nutritional supplement containing the above-mentioned ceramide-containing composition as a main component. It may be food.

When producing the food and drink of the present embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soy protein and corn protein; amino acids such as alanine, glutamine and isoleucine; Sugars: Any auxiliary agent such as oils and fats such as soybean oil and medium-chain amino acid triglyceride can be added to prepare foods and drinks having any shape.

The food and drink to which the above ceramide-containing composition can be blended is not particularly limited, and specific examples thereof include beverages such as soft beverages, carbonated beverages, nutritional beverages, fruit beverages, and lactic acid beverages (concentrated stock solutions and preparations for these beverages). Includes powders); Cold drinks such as ice cream, ice sherbets, and shaved ice; Noodles such as buckwheat, udon, harusame, gyoza skin, sushi skin, Chinese noodles, and instant noodles; candy, chewing gum, candy, gum, chocolate, Confectionery such as tablets, snacks, biscuits, jelly, jams, creams, baked confectionery; marine and livestock processed foods such as kamaboko, ham, sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, Oils and fats and processed foods such as mayonnaise, shortening, whipped cream, dressings; seasonings such as sauces and sauces; soups, stews, salads, prepared foods, pickles; other various forms of health and nutritional supplements; tablets, capsules, Examples thereof include drinks, and when the above-mentioned ceramide-containing composition is blended with these foods and drinks, auxiliary raw materials and additives usually used can be used in combination.

Since the above-mentioned food and drink according to the present embodiment contains a ceramide-containing composition extracted with an aqueous solvent containing a surfactant that can be used as a food additive and does not contain an organic solvent, ceramide. The favorable action of the class is imparted, and the safety is excellent.

Hereinafter, the present invention will be specifically described with reference to Examples, Test Examples and the like, but the present invention is not limited to the following examples.

[Example 1]
52 g of MRS medium (manufactured by Merck Millipore) was dissolved in 1000 g of water and heat-sterilized, and 0.1 g of yeast (Saccharomyces verona, manufactured by Akita Konno Shoten) was inoculated into the flask. The culture was carried out by allowing it to stand for a while. The obtained culture broth was centrifuged (2,500 × g, 30 minutes) to remove the culture medium supernatant, and yeast cells were collected.

300 g of the obtained bacterial cells was added to 3,000 g of water containing 0.1% by mass of Tween 20 (polyoxyethylene (20) sorbitan monolaurate) (manufactured by NOF CORPORATION), and the mixture was extracted at 80 ° C. The obtained extract was filtered through diatomaceous earth and concentrated under reduced pressure to obtain a ceramide-containing composition as an extract (2,800 g).

[Example 2]
A ceramide-containing composition (2,800 g) was obtained in the same manner as in Example 1 except that the concentration of Tween 20 as a surfactant was changed to 0.2% by mass.

[Example 3]
A ceramide-containing composition (2,800 g) was obtained in the same manner as in Example 1 except that the concentration of Tween 20 as a surfactant was changed to 0.01% by mass.

[Example 4]
A ceramide-containing composition (2,800 g) was obtained in the same manner as in Example 1 except that the concentration of Tween 20 as a surfactant was changed to 0.05% by mass.

[Comparative Example 1]
An extract (2,800 g) was obtained in the same manner as in Example 1 except that water containing no Tween 20 was used as the extraction solvent.

[Test Example 1] Detection of ceramides Ceramides were detected in the extracts (ceramide-containing compositions) obtained in Examples and Comparative Examples as follows.

25 mL of distilled water was added to 5 mL of the extracts (ceramide-containing compositions) obtained in Examples and Comparative Examples to dilute them, and the mixture was passed through Sep-Pak (manufactured by Waters) and adsorbed on a column. After washing this column with 6 mL of water, glucosylceramide was eluted with 6 mL of methanol. The eluate was concentrated under reduced pressure, and 0.5 mL of methanol was added to the concentrate to dissolve it to prepare a sample solution. On the other hand, 2 mg of a glucosylceramide standard product (derived from corn) (manufactured by Nagara Science Co., Ltd., NS170202) was collected and dissolved in 10 mL of methanol to prepare a standard solution.

The obtained sample solution and standard solution were subjected to thin layer chromatography (TLC) under the following conditions, and light purple-brown to purple-brown spots were observed at an Rf value of about 0.3.

<Thin layer chromatography conditions>
Developing solvent: Ethyl acetate / 1-propanol / water mixture (80: 20: 3)
Thin layer plate: Pre-Coated TLC plates SILICAGEL 60F254 (manufactured by Merck)
Deployment distance: Approximately 6 cm
Detection method: Anthrone sulfuric acid solution in which 0.1 g of anthrone and 2 g of thiourea are dissolved in 200 mL of diluted sulfuric acid is evenly sprayed on the developed thin layer plate, and then heated at 120 ° C. for 10 minutes.

The results of TLC analysis on the samples of Examples 1 and 2 are shown in FIG. 1, and the results of Examples 1, 3 and 4 and Comparative Example 1 are shown in FIG. 2, respectively.

As shown in FIGS. 1 and 2, it was clarified that ceramides can be efficiently extracted by adding a surfactant to water as an extraction solvent without using an organic solvent. Here, it is clear that the composition of Example 1 extracted with an aqueous solvent having a surfactant content of 0.1% by mass has the highest content of ceramides and a small amount of other impurities. became.

[Formulation Example 1]
A milky lotion was produced by a conventional method according to the following composition.
Ceramide-containing composition (Example 1) 0.01 g
Jojoba oil 4.00g
1,3-butylene glycol 3.00g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50 g
Olive oil 2.00g
Squalan 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00 g
Polyoxyethylene sorbitan oleate (20E.O.) 2.00 g
Methyl paraoxybenzoate 0.15 g
Stearyl glycyrrhizinate 0.10 g
Yellow 杞 extract 0.10g
Dipotassium glycyrrhizinate 0.10 g
Ginkgo leaf extract 0.10g
Conchiolin 0.10g
Phellodendron extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water balance (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Ceramide-containing composition (Example 1) 0.05 g
Kujin extract 0.1g
Scutellaria extract 0.1g
Liquid paraffin 5.0g
Sarashi beeswax 4.0g
Squalene 0.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Polyoxyethylene sorbitan oleate (20E.O.) 1.5g
Glyceryl monostearate 3.0 g
Oil-soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
Methyl paraoxybenzoate 1.5g
Fragrance 0.1g
Purified water balance (total amount is 100 g)

[Formulation Example 3]
A beauty essence having the following composition was produced by a conventional method.
Ceramide-containing composition (Example 1) 0.01 g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethyl cellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
Dipotassium glycyrrhizinate 0.1g
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (methyl paraoxybenzoate) 0.15 g
Ethanol 2.0g
Purified water balance (total amount is 100 g)

[Formulation Example 4]
A hair tonic having the following composition was produced by a conventional method.
Ceramide-containing composition (Example 1) 0.4 g
Tocopherol acetate Appropriate amount Cephalatin 0.002 g
Isopropylmethylphenol 0.1g
Sodium hyaluronate 0.15g
Glycerin 15.0g
Ethanol 15.0g
Fragrance Appropriate amount Chelating agent (sodium edetate) Appropriate amount Preservative (hinokitiol) Appropriate amount Solubilizer (polyoxyethylene cetyl ether) Appropriate amount Purified water balance (total amount is 100 g)

[Formulation Example 5]
A shampoo having the following composition was produced by a conventional method.
Ceramide-containing composition (Example 1) 0.5 g
Marjoram extract 1.0g
Plum fruit extract 0.2g
Coconut oil fatty acid methyl taurine sodium 10.0g
Coconut oil fatty acid amide propyl betaine 10.0g
Polyoxyethylene alkyl ether sodium sulfate 20.0 g
Coconut oil fatty acid diethanolamide 4.0g
Propylene glycol 2.0g
Perfume Appropriate amount Purified water balance (total amount is 100g)

[Formulation Example 6]
Tablets having the following composition were produced by a conventional method.
Ceramide-containing composition (Example 1) 5.0 mg
Dolomite (containing 20% calcium and 10% magnesium) 83.4 mg
Casein phosphopeptide 16.7 mg
Vitamin C 33.4 mg
Maltitol 136.8 mg
Collagen 12.7 mg
Sucrose fatty acid ester 12.0 mg

[Formulation Example 7]
An oral liquid preparation having the following composition was produced by a conventional method.
<Composition in 1 ampoule (100 mL per ampoule)>
Ceramide-containing composition (Example 1) 0.3% by mass
Sorbitol 12.0% by mass
Sodium benzoate 0.1% by mass
Fragrance 1.0% by mass
Calcium sulfate 0.5% by mass
Purified water balance (100% by mass)

According to the present invention, a composition containing ceramides, which is highly useful as a compounding ingredient for cosmetics, foods and drinks, pharmaceuticals, etc., can be extracted from yeast with an aqueous solvent containing no organic solvent, and is safe. It is possible to produce a ceramide-containing composition having a high content.

Claims (6)

A method for producing a ceramide-containing composition for extracting yeast using an aqueous solvent containing a surfactant without containing an organic solvent.
The surfactant comprises a polyoxyethylene sorbitan fatty acid ester
The temperature in the extraction is 50 to 100 ° C.
A method for producing a ceramide-containing composition. The method for producing a ceramide-containing composition according to claim 1, wherein the aqueous solvent contains 0.01 to 2% by mass of the surfactant. The method for producing a ceramide-containing composition according to claim 1 or 2, wherein the yeast is Saccharomyces veronae. A ceramide-containing composition produced by the production method according to any one of claims 1 to 3. A cosmetic comprising the ceramide-containing composition according to claim 4. A food or drink containing a ceramide-containing composition produced by the production method according to any one of claims 1 to 3.
The surfactant is at least one or more selected from the group consisting of propylene glycol fatty acid ester, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, and polyoxyethylene sorbitan fatty acid ester. Contains the polyoxyethylene sorbitan fatty acid ester
Food and drink characterized by that.

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