JP2007045732A - Disinfection solution, and article for disinfection - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a disinfection solution mild to the skin and having effects for inactivating Norovirus. <P>SOLUTION: The antiseptic solution contains 0.05-0.5 wt.% polyhexamethylene biguanide-based compound and has a pH within the range of 9-12. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a disinfecting solution having antibacterial properties and a disinfecting article impregnated with the disinfecting solution.

  Food poisoning caused by norovirus infection occurs frequently in the winter, accounting for 38% of food poisoning cases, and is the most important cause of food poisoning cases. Norovirus is an RNA virus that does not have an envelope with a diameter of about 30 nm (3 × 10 −8 m) and is classified into the Caliciviridae and Norovirus genus, and has a strong resistance to acidity (gastric acid).

  Moreover, it is known that it will infect with a small amount (about 10-100 intake). The main route of infection is oral infection by eating shellfish such as raw oysters, the main causative food, but many other cases have been reported through the fingers and cooking utensils of people who touched contaminated food. ing.

  In addition, secondary infections with large amounts of virus in patient vomit and feces have been reported. This is because the virus is excreted in the stool for several days to several weeks after the patient recovers, and this is an infection caused by viruses attached to the patient's surrounding environment (doors, curtains, linens, daily necessities, etc.). Is presumed to be

  The incubation period of norovirus is usually 12 to 72 hours, causing inflammation in the intestinal tract of small intestinal epithelial cells. The main symptom is diarrhea associated with inflammation of the small intestine.

  Norovirus is very infectious and retains infectivity for many years in nature. For example, at a refrigerator temperature of 4 ° C., the infectivity is maintained for several months although the infectivity is reduced. 2-3 weeks at room temperature. At 37 degrees, it is estimated to die around 10 days. Moreover, it has a strong physicochemical resistance, is resistant to chlorine disinfection usually used for disinfection of tap water and pool water, and it is considered that 200 ppm or more is necessary for inactivation of rapid action. There is also resistance to alcohol, and it is said that inactivation requires treatment with 70% alcohol for 5 to 30 minutes or longer. Furthermore, it is highly resistant to heat and is not inactivated by treatment at 56 ° C. for 30 minutes.

Due to the above characteristics of norovirus, ethanol and reverse soap (such as benzalkonium chloride) that are highly effective against many bacteria are also less effective against norovirus. At present, boiling and sodium hypochlorite (concentration of 200 ppm or more) are the most effective inactivation means.
JP 2004-529148 A JP 2004-188091 A

  However, boiling treatment cannot be used in places where heat-sensitive materials and water cannot be handled in large quantities. In addition, sodium hypochlorite has a drawback that the object to be treated is limited because it rusts the metal.

  Accordingly, there is a need for a drug treatment method that can inactivate Norovirus in a short time, with less restrictions on use and more convenient for preventing Norovirus infection.

  The applicant has developed a wet tissue “alcohol towel that can be sterilized” using a non-causative agent of food poisoning such as O-157, Sanemonella and Staphylococcus aureus, and a bactericidal alcohol preparation such as influenza virus. It is provided to welfare facilities, etc., but this towel could not inactivate norovirus in a short time.

  Therefore, a main problem of the present invention is to provide a disinfectant that is highly safe and can inactivate norovirus in a short time.

  In order to solve the above problems, the present inventors have studied various existing drugs, but there is no powerful drug that can inactivate norovirus while satisfying the requirements of safety and gentleness to the hand skin. . Therefore, as a result of intensive studies on the disinfectant having the inactivating effect of norovirus, the present inventors surprisingly contain 0.05 to 0.5% by weight of polyhexamethylene biguanide compound, and the pH is It was found that the disinfecting liquids 9 to 12 exert the effect of inactivating norovirus even in a small amount. Moreover, it has been found that such a disinfectant is gentle and safe for the hand skin.

  The polyhexamethylene biguanide compound exhibits a virus inactivating effect even in a small amount. The reason for this is not clear, but it is thought that the number of reactive groups (NH groups) per structural unit is large, and the attack probability against viruses increases. And since the polyhexamethylene biguanide type compound is gentle and safe for the skin, it was found that it is suitable for the disinfection of the skin or the part that directly touches the skin.

  Furthermore, the present inventors have found that when the disinfectant contains 40 to 80% by weight of alcohol, the effect of inactivating norovirus is further enhanced. Since such a disinfectant is gentle to the skin and safe as described above, it can be used as a disinfecting article impregnated in the base material. In this way, convenience is enhanced.

  As described above, according to the present invention, it is possible to obtain a disinfecting solution and a disinfecting article that are gentle and safe to the skin and have an effect of inactivating norovirus.

Hereinafter, an embodiment of the present invention will be described in detail with reference to the accompanying drawings.
<About disinfectant>
The disinfecting solution of the present invention contains 0.05 to 0.5% by weight of a polyhexamethylene biguanide compound and has a pH in the range of 9 to 12. The polyhexamethylene biguanide compound is a polymer in which a large number of biguanide groups and hexamethylene groups are connected alternately as shown in the following chemical formula 1, and is usually sold in the form of hydrochloride. In the chemical formula, n is an appropriate integer. In the present invention, n = 3 to 16, particularly n = 3 to 7 or n = from the viewpoint of availability, cost effectiveness, ease of handling, and the like. 10 to 16 such compounds are suitable.

  The content of the polyhexamethylene biguanide compound in the disinfectant is 0.05 to 0.5% by weight. If the content of the polyhexamethylene biguanide compound is less than 0.05% by weight, the inactivation effect of norovirus tends to be insufficient, and if it exceeds 0.5% by weight, there is no significant change in the virus inactivation effect. The gentleness and safety of the camera are reduced. In addition, it cannot be overemphasized that the amount of each component in a disinfection liquid can be determined by the addition amount or the component quantitative analysis of the disinfection liquid squeezed from the product base material.

  In the inactivation of the norovirus by the polyhexamethylene biguanide compound, the pH of the disinfectant is very important, and is preferably 9 to 12, particularly 9.1 to 10.8. Outside this range, the virus inactivation effect is poor. The pH of the disinfectant can be adjusted by using an appropriate amount of a pH adjusting agent such as sodium chloride or sodium hydroxide. In particular, it is desirable to adjust with a glycine buffer. The content of the pH adjusting agent in the disinfecting solution can be determined as appropriate according to the target pH, but is usually less than 15% by weight. In the case of a glycine buffer, the ionic strength is preferably from 0.01 to 0.5 mol, particularly preferably from 0.05 to 0.2 mol.

  Alcohol can be added to the disinfecting solution of the present invention in order to improve the antibacterial effect and the accompanying norovirus inactivation effect. As the alcohol, for example, ethanol, propanol, butanol, isopropanol and the like can be used. Moreover, as glycol, propylene glycol, dipropylene glycol, 1, 3- butylene glycol, etc. can be used. The alcohol content in the disinfectant can be determined as appropriate, but is preferably 40 to 80% by weight. If the alcohol content is less than 40% by weight, the antibacterial effect is insufficient, and if it exceeds 80% by weight, there is no significant change in the antibacterial effect, and the cost effectiveness deteriorates.

  In the disinfecting solution of the present invention, other antibacterial components other than the polyhexamethylene biguanide compound can be contained. Examples of other antibacterial components include benzalkonium chloride, cetylbilidinium chloride, phenoxyethanol, chlorohexidine gluconate, lauryl sulfate, and the like. The content of other antibacterial components in the disinfectant may be appropriately determined according to the medicinal effect, but is usually preferably 0.01 to 0.3% by weight. When the content is less than 0.01% by weight, the antibacterial effect by the antibacterial component becomes insufficient, and when it exceeds 0.3% by weight, the antibacterial effect and its sustainability are not significantly changed, and the cost effectiveness is deteriorated.

  Although it does not specifically limit as a solvent used for the disinfection liquid of this invention, Water is suitable.

  In addition to the above, the disinfecting solution of the present invention may contain trace components such as humectants such as aloe extract and glycine, and preservatives such as methylparaben, ethylparaben and propylparaben. The content of this trace component can be, for example, about 0.1 to 0.3% by weight.

  On the other hand, the content of the disinfecting solution of the present invention with respect to the base material can be determined as appropriate. For example, in the case of a non-woven fabric base material, the content is preferably about 200 to 400% with respect to the absolute dry weight of the base material. If the content with respect to the substrate is less than 200%, the liquid content is small and dirt is difficult to remove, and if it exceeds 400%, the surface to be wiped becomes difficult to dry.

<About the base material>
The base material that can be used in the wiping article of the present invention is not particularly limited as long as it is made of a fiber assembly, and paper, non-woven fabric, woven fabric, etc. can be used, and it has water decomposability. In addition to those not present, those having water decomposability can also be used.

  The fiber constituting the substrate can be used regardless of natural, regenerated, or synthesized. In the present invention, fibers containing at least hydrophilic fibers are preferable. Examples of hydrophilic fibers that can be used include natural fibers such as cotton and pulp, and regenerated fibers such as rayon and cupra. Among these fibers, rayon is particularly preferable. Rayon is rich in water absorption and is easy to handle, and a certain length of fiber can be obtained at low cost. Such hydrophilic fibers are desirably blended in the substrate at a content ratio of 50 to 70% by weight. When the content of the hydrophilic fiber is less than 50% by weight, sufficient flexibility and water retention cannot be given, and when it exceeds 70% by weight, the strength when wet is too low, and the tearing is caused. In addition to being easily generated, it becomes excessively stretched when taken out from the container in a pop-up manner.

  In the base material of the present invention, in addition to the above hydrophilic fibers, heat-fusible fibers can be used to bond the fibers together. As the heat-fusible fiber, any fiber that melts by heating and exhibits adhesiveness to each other can be used. This heat-fusible fiber may be a single fiber or a composite fiber in which two or more synthetic resins are combined. Specific examples include polyolefin single fibers such as polyethylene, polypropylene and polyvinyl alcohol, polyethylene terephthalate / polyethylene, polyethylene terephthalate / polypropylene, polypropylene / polyethylene, polyethylene terephthalate-ethylene / propylene copolymer, low-melting point polyester-polyester, etc. A core-sheath type composite fiber or an eccentric core-sheath type composite fiber whose sheath part is made of a relatively low melting point, or a part of each component made of polyethylene terephthalate / polypropylene, polyethylene terephthalate / nylon, polypropylene / polyethylene is the surface Splitting by heat shrinkage of one component consisting of split type composite fiber or polyethylene terephthalate / ethylene-propylene copolymer exposed in Or the like can be used composite fibers. In this case, a core-sheath type composite fiber is preferable when productivity and dimensional stability are important, and an eccentric type composite fiber is preferable when importance is placed on volume. In addition, if importance is attached to flexibility, when a split type composite fiber or a heat split type composite fiber is used, each component can be easily divided into ultrafine fibers by high-pressure water flow treatment. Such heat-fusible fibers are desirably blended at a content ratio of 10 to 30% by weight. When the heat-fusible fiber is less than 10% by weight, the wet strength cannot be secured, and the elongation becomes too large when the pop-up type is taken out from the container. On the other hand, if it exceeds 30% by weight, the texture becomes hard and the feel becomes rough, which is not preferable for this type of wet tissue.

  Moreover, in the base material of this invention, a thermoplastic synthetic fiber other than a hydrophilic fiber and a heat-fusible fiber can be mixed. As the thermoplastic fiber, various synthetic fibers exist, and among them, a polyester fiber is preferable. Polyolefin fibers such as polyethylene and polypropylene have poor confounding properties when subjected to high-pressure water flow treatment, and become inferior in fluffing and wet strength. Also, polyamide fibers such as nylon are hydrophilic and are not preferred. The mixing amount of the thermoplastic fibers is preferably 40% by weight or less. When the content of the thermoplastic fiber exceeds 40% by weight, the water retention is impaired and the wet performance is remarkably lowered. By mixing thermoplastic fibers, the stiffness when wet is improved and a bulky nonwoven fabric can be obtained. Moreover, when a part is exposed to the nonwoven fabric surface, a sticky feeling is relieved even when wet due to hydrophobicity, and a dry feel is imparted.

  Desirably, the thermoplastic fiber has thermal crimpability. In order to impart heat crimpability, a form of side-by-side type composite fiber in which synthetic resins having different heat shrinkage temperatures are bonded to thermoplastic fibers is adopted. For example, the melting point of the polyester fiber is 255 ° C. for polyethylene terephthalate and 215 ° C. for polybutylene terephthalate. As the low melting point resin to be bonded to this, a resin substantially the same as the melting point temperature of the above-mentioned heat-fusible fiber is used. It is better to do so.

In the present invention, there is no particular limitation on the shape of the base material, and it may have a thick block shape in addition to the sheet shape. In the case of a sheet-like substrate, the basis weight of the substrate is preferably ²⁰ to 80 g / m ² , particularly about 30 to 60 g / m ² . When the basis weight of the base material is less than 30 g / m ² , the ability to retain dirt becomes poor and it becomes difficult to provide unevenness as described later, and when it exceeds 60 g / m ² , the flexibility becomes poor.

  On the other hand, the substrate 1 may have a flat surface. However, in order to make the sustainability of the effect more excellent, as shown in FIG. 1 and FIG. Is preferably formed. In this case, the dirt scraping effect is improved by the convex portion 2, and the dirt accommodation effect is improved by the concave portion 3. As a result, the effect of removing dirt on the surface to be wiped is improved, and the unevenness of the dirt becomes difficult to remain, so that volatilization of the disinfecting liquid is suppressed. FIG. 1 shows an example in which irregularities are formed on both surfaces of the substrate 1, and FIG. 2 shows an example in which irregularities are formed on one surface of the substrate 1.

  As shown in FIG. 1 and FIG. 2, a particularly preferable form is that the surface of the substrate 1 has convex portions 2, 2... Having a relatively high fiber density and concave portions 3, 3. Many are formed. In this case, the scraping ability is improved by increasing the rigidity of the convex portion 2, and the dirt accommodation capability is improved by widening the fiber gap of the concave portion 3. Moreover, since the base material 1 having such irregularities includes the partial convex portions 2 having a high fiber density and the partial concave portions 3 having a low fiber density, the base material 1 is structurally easy to bend in the concave portions 3 having a low fiber density. In addition, moderate softness is imparted, and an appropriate texture is imparted by the uneven pattern on the surface.

  More specifically, in the base material 1 in the illustrated example, the ridges 2, 2... Are formed with a relatively high fiber density and a linear shape, and are formed with a relatively low fiber density and a linear shape. The recessed ridges 3, 3, ... are present alternately. The irregularities are preferably formed alternately as in the illustrated example, but the present invention is not limited to this and can be irregularly formed. Moreover, although the unevenness is preferably formed in a strip shape as in the illustrated example, it can also be formed in a dot shape.

  If the strip-shaped unevenness is formed in parallel and alternately as in the example shown in FIG. 3, the scraping effect by the convex portion and the accommodating effect by the concave portion are not exhibited at the time of wiping in the extending direction. Therefore, as shown in FIG. 4, the linear pattern formed by the ridges 2 and the recesses 3 intersects the linear pattern formed by the other ridges 2 and the recesses 3. It is preferable to present an intersecting pattern (in the illustrated example, a herringbone pattern). In this case, no matter which direction is wiped off, dirt is pushed into the recess 3 due to the presence of the protrusion 2, and finally the dirt is scooped out by the protrusion 2, so that it is beautiful. Can be wiped off.

  As the cross pattern of the linear pattern formed by the ridge portion 2 and the concave stripe portion 3, it is sufficient that the linear pattern intersects with other linear patterns. Various patterns such as a rhombus pattern can be used. It is desirable that the number of lines of the linear pattern formed by the ridges 2 and the recesses 3 is 3 to 9 / cm. When the number of lines is less than 3 / cm, the non-woven fabric becomes almost flat, so that once captured, the dirt is easily transferred, and when the number of lines exceeds 9 / cm, the protrusion 2 Since the volume of the space formed by the groove portion 3 becomes too small, a desired amount of dirt cannot be secured in the groove portion 3, which is not desirable.

  Moreover, as FIG.1 and FIG.2 shows, the height (thickness) Hm from the back surface of the protruding item | line part 2 is 300-800 micrometers, Preferably it is 450-650 micrometers, The height from the back surface of the recessed item part 3 ( Thickness Hd is 100 to 500 μm, preferably 200 to 400 μm. From another viewpoint, it is desirable that the height difference between the ridges 2 and the ridges 3 is 50 to 300 μm, preferably about 75 to 150 μm. When the height difference is less than 50 μm, a large amount of capturing effect by the concave strip portion 3 cannot be expected and a desired wiping amount cannot be secured. In addition, when the height difference exceeds 300 μm, the thickness of the base material increases as a result, and the flexibility and the touch feeling are impaired.

  On the other hand, the base material 1 having the unevenness described above can be manufactured, for example, as follows. That is, a fiber web comprising a hydrophilic fiber such as rayon, a heat-fusible fiber such as polyethylene or polypropylene, and a thermoplastic fiber such as polyester fiber on a wire mesh having a desired surface irregularity pattern to be applied to the substrate. And a high-pressure water stream is jetted from above the fiber web during conveyance so that the fibers are entangled. At this time, the fiber in the portion of the wire mesh where the wire is present is pushed to the both sides of the wire by the collision energy of the high-pressure water stream and moved to the opening side, so that the fiber portion of the mesh opening portion has a relatively high fiber density. While becoming higher, it becomes convex according to the shape of the opening, while the portion where the wire is present has a relatively low fiber density and a concave shape is formed along the wire.

  The fibers are entangled with each other by the high-pressure water flow, and the entire fiber web is integrated. Thereafter, heat treatment is performed on the integrated fiber web at a temperature close to the melting point of the heat-fusible fiber, the fibers are bonded to each other by melting the heat-fusible fiber, and the thermoplastic fiber has heat crimpability. Bulkiness is provided by crimping.

  The above manufacturing method is an application of the spunlace method. However, other methods such as a chamber method, a dry method, a spunbond method, a melt blow method, a needle punch method, and a stitch bond method are used. Needless to say, it can be applied to the production of the substrate of the present invention as long as it can be formed.

In order to confirm the effect of the present invention, a norovirus inactivation experiment was conducted.
(Content of inactivation experiment)
Using 15 mL polyethylene tube (assist), FCV 0.3 mL was added to each disinfectant (Examples and Comparative Examples) (9: 1), and after 5 minutes, 50 μL of sample was taken and added to 5 mL DMEM (described later), The effect of the drug solution was neutralized by diluting 100 times, and the virus titer was immediately diluted 10 times to measure the virus titer. The experiment was performed at room temperature.

(Virus titration measurement method)
The plaque method was used as the virus titer measurement method. Specifically, 100 μL was inoculated using 2 to 4 holes for each 10-fold serial dilution in a 6-well microplate (Falcon, USA), adsorbed at 36 ° C. for 1 hour, and then overlaid with 1% methylcellulose. The macroplate was placed in a 5% carbon dioxide furan vessel and cultured at 36 ° C. for 2 days. Thereafter, the plate was fixed with 10% formalin, stained with methylene blue, and the number of plaques was measured.

(Cells, etc.)
As the cells, Crandell-Reese Feline Kindny (CRFK) cells were used. For cell growth, Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum, 100 u / mL penicillin, 100 μg / mL streptomycin, and 1.5% sodium bicarbonate was used. To maintain cells, 2% of the same serum was added. The culture was performed at 36 ° C. in a 5% CO ² furan vessel.

(Virus)
Feline calicivirus (FCV) F4 strain was used. Virus grown on CRFK was freeze-thawed twice, dispensed into 0.5 mL, and stored at -74 ° C until use.

(Antiseptic solution)
The chemical | medical solution of Examples 1-8 and Comparative Examples 1-14 was used for disinfection liquid. The composition of each chemical solution is as shown in the following Tables 1 to 4 shown together with the results. Table 1 shows the difference in virus inactivation effect between the preferred embodiment of the present invention and the comparative example, Table 2 shows the relationship between the pH of the chemical solution and the inactive effect, and Table 3 shows polyhexamethylene biguanide (PHMB) in the chemical solution. ) Relationship between concentration and virus inactivation effect, Table 4 shows the virus inactivation effect when ethanol is mixed.

(Results and discussion)
As can be seen from Tables 1 to 4, Examples (including polyhexamethylene biguanide compounds and having a pH in the range of 9 to 12) which are disinfectants according to the present invention have a virus titer after 5 minutes at the limit of measurement. However, in the comparative example, a sufficient inactivation effect is not obtained, while an inactivation effect of norovirus is obtained and an immediate effect is recognized.

  And as can be seen from Table 2, this inactivation effect of Norovirus is obtained in the pH range of about 9 to 12, and is not recognized outside this range. Moreover, when pH exceeds 12, irritation | stimulation to skin will become strong (Comparative Example 7).

  Further, as can be seen from Table 3, in the comparative example in which the amount of polyhexamethylene biguanide compound is less than 0.1, a sufficient inactivation effect is not obtained, and the amount of mixture is 1.0% by weight. Although inactivating effect is obtained, the skin is strongly stimulated. Although the inventors have not shown in the table, when the amount of the polyhexamethylene biguanide compound is 0.6 or more, irritation to the skin begins to be felt strongly and the cost effectiveness deteriorates. I know.

  Further, as can be seen from Table 4, the inactivation effect of norovirus can be obtained even if ethanol is included. Since ethanol has a general disinfection effect, it is recognized that it is effective against bacteria other than norovirus. In addition, although it cannot read from Table 4, it has discovered that the irritation | stimulation to skin becomes strong from the content of ethanol exceeding 80% (refer comparative example 14). Moreover, the ethanol inactivation effect cannot be obtained (see Example 4 in Table 1).

  As a result, it can be said that the disinfecting solution according to the present invention has an inactivating effect of norovirus, has an immediate effect, and is less irritating to the skin.

  INDUSTRIAL APPLICATION This invention can be utilized for the disinfection use of the norovirus adhering to the skin or the goods which skin touches.

It is sectional drawing of the base material which has an unevenness | corrugation. It is sectional drawing of another base material. It is a perspective view of another base material. It is a top view of another base material.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Base material, 2 ... Convex part, 3 ... Concave part.

Claims (3)

  A disinfecting solution comprising 0.05 to 0.5% by weight of a polyhexamethylene biguanide compound and having a pH in the range of 9 to 12.   The disinfectant according to claim 1, comprising 40 to 80% by weight of alcohol.   An article for disinfection, wherein a base material is impregnated with the disinfectant solution according to claim 1 or 2.

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