WO2017163739A1

WO2017163739A1 - Disinfecting solution against noroviruses and disinfecting article against noroviruses

Info

Publication number: WO2017163739A1
Application number: PCT/JP2017/006715
Authority: WO
Inventors: 広大氏家
Original assignee: 大王製紙株式会社
Priority date: 2016-03-23
Filing date: 2017-02-23
Publication date: 2017-09-28
Also published as: JP2017171606A; TW201734190A; TWI798172B

Abstract

[Problem] To provide a disinfecting solution against noroviruses and a disinfecting article against noroviruses which have high safety and can inactivate noroviruses within a short period of time. [Solution] A disinfecting solution against noroviruses comprises 0.05-0.5 wt% of a polyhexamethylene biguanide-based compound and 40-80 wt% of an alcohol together with sodium carbonate and sodium hydrogen carbonate and has a pH value adjusted to 9-12. A disinfecting article against noroviruses is prepared by impregnating a base material comprising a fiber assembly with the aforesaid disinfecting solution.

Description

Norovirus disinfectant and norovirus disinfecting article

The present invention relates to an antibacterial norovirus disinfectant and an article for norovirus disinfection impregnated with the norovirus disinfectant.

Food poisoning caused by norovirus infection occurs frequently in the winter, accounting for 38% of food poisoning cases, and is the most important cause of food poisoning cases. Norovirus is an RNA virus having a diameter of about 30 nm (3 × 10 ⁻⁸ m), which is classified into the Caliciviridae and Norovirus genera, and has a strong resistance to acidity (gastric acid).

Also, it is known to be infected with a small amount (ingestion of about 10 to 100). The main route of infection is oral infection by eating shellfish such as raw oysters, the main causative food, but many other cases have been reported through the fingers and cooking utensils of people who touched contaminated food. ing.

In addition, secondary infections due to large amounts of virus contained in the patient's vomit and feces are also reported. This means that even if the patient recovers, the virus is discharged into the stool for several days to several weeks, so it is an infection caused by the virus attached to the patient's surrounding environment (door knobs, curtains, linens, daily necessities, etc.) Has been speculated.

The incubation period of norovirus is usually 12 to 72 hours, causing inflammation in the intestinal tract of small intestinal epithelial cells. The main symptom is diarrhea associated with inflammation of the small intestine.

¡Norovirus has a very strong infectivity and retains infectivity for a long time in nature. For example, at a refrigerator temperature of 4 ° C., the infectivity is maintained for several months although the infectivity is reduced. It is estimated that it will die within 2-3 weeks at room temperature and around 10 days at 37 degrees. Moreover, it has a strong physicochemical resistance, is resistant to chlorine disinfection usually used for disinfection of tap water and pool water, and it is considered that 200 ppm or more is necessary for inactivation of rapid action. It has resistance to alcohol, and it is said that inactivation requires treatment with 70% alcohol for 5 to 30 minutes or longer. Furthermore, it is highly resistant to heat and is not inactivated by treatment at 56 ° C. for 30 minutes.

Due to the characteristics of Norovirus as described above, ethanol and reverse soap (such as benzalkonium chloride) that are highly effective against many bacteria are also less effective against Norovirus. At present, boiling and sodium hypochlorite (concentration of 200 ppm or more) are the most effective inactivation means.

However, boiling treatment cannot be used in places where heat-sensitive materials and water cannot be handled in large quantities. In addition, sodium hypochlorite has a drawback that the object to be treated is limited because it rusts the metal. Therefore, there has been a demand for a drug treatment method that can reduce the use of norovirus in a short time, with less restrictions on the use, and prevention of norovirus infection.

In this regard, the present applicant has developed a wet tissue “alcohol towel that can be sterilized” using an antiseptic solution that is effective against food poisoning bacteria such as O-157, Sanemonella, Staphylococcus aureus, and influenza viruses. However, this towel has not been able to inactivate norovirus in a short time.

Therefore, the present applicant has proposed a norovirus disinfectant containing a polyhexamethylene biguanide compound and an alcohol, having a pH in the range of 9 to 12, and using a glycine buffer as a pH adjuster (Patent Document 1). .

However, when a glycine buffer is used as a pH adjuster, it is usually necessary to use sodium hydroxide, a strong base similar to this, or a strong acid. Therefore, it has been sought whether it is possible to improve the safety of manufacturing workers without using these.

Japanese Patent No. 4975987

The main problem to be solved by the present invention is to provide a norovirus disinfecting solution and an article for norovirus disinfection that are highly safe and can inactivate norovirus in a short time.

Means for solving the above problems are as follows.
(Aspect of Claim 1)
Containing 0.05 to 0.5% by weight of polyhexamethylene biguanide compound and 40 to 80% by weight of alcohol;
And containing sodium carbonate and sodium bicarbonate,
the pH is in the range of 9-12,
Norovirus disinfectant characterized by that.

(Aspect of Claim 2)
The sodium carbonate content is 0.01-0.3 wt%,
The content of the sodium bicarbonate is 0.08 to 0.8% by weight,
The norovirus disinfecting solution according to claim 1.

(Aspect according to claim 3)
The disinfecting solution according to claim 1 or claim 2 is impregnated in a substrate made of a fiber assembly.
Norovirus disinfecting article characterized by the above.

(Main effects)
A disinfecting solution containing 0.05 to 0.5% by weight of polyhexamethylene biguanide compound (PHMB) and having a pH of 9 to 12 exhibits an effect of inactivating norovirus even in a small amount.

In this regard, the polyhexamethylene biguanide compound exhibits the virus inactivating effect even in a small amount. The reason for this is not clear, but it is thought that the number of reactive groups (NH groups) per structural unit is large, and the attack probability against viruses increases.

Since polyhexamethylene biguanide compounds are gentle and safe for the skin, they are suitable for disinfecting the skin or directly touching the skin.

In addition, when the disinfectant contains 40 to 80% by weight of alcohol, the effect of inactivating norovirus is further increased. Such a disinfectant can be used as a disinfecting article impregnated in a base material, and in this way, convenience is enhanced.

Further, the same effect can be obtained by adding a buffer solution of sodium carbonate and sodium hydrogen carbonate (a combination of weak acid salt and normal salt) instead of the glycine buffer as a pH adjuster. And when the said buffer solution is used, since it becomes unnecessary to use sodium hydroxide, the strong base similar to this, or a strong acid, the safety of a manufacturing operator can be improved.

According to the present invention, a norovirus disinfecting solution and an article for norovirus disinfection that are highly safe and can inactivate norovirus in a short time.

Hereinafter, an embodiment of the present invention will be described in detail.
<About disinfectant>
The disinfecting solution of this embodiment contains 0.05 to 0.5% by weight of a polyhexamethylene biguanide compound and has a pH in the range of 9 to 12. A polyhexamethylene biguanide compound is a polymer in which a large number of biguanide groups and hexamethylene groups are alternately connected as shown in the following chemical formula 1, and is usually sold in the form of hydrochloride.

In the chemical formula, n is an appropriate integer. In this embodiment, n = 3 to 16, particularly n = 3 to 7, or n = 10 to n from the viewpoints of availability, cost effectiveness, and ease of handling. Sixteen such compounds are suitable.

The content of the polyhexamethylene biguanide compound in the disinfectant is 0.05 to 0.5% by weight. If the content of the polyhexamethylene biguanide compound is less than 0.05% by weight, the inactivation effect of norovirus tends to be insufficient, and if it exceeds 0.5% by weight, there is no significant change in the virus inactivation effect. The gentleness and safety of the camera are reduced. The amount of each component in the disinfecting solution can be determined by the amount of addition or by component quantitative analysis of the disinfecting solution squeezed from the product substrate.

In the inactivation of the norovirus by the polyhexamethylene biguanide compound, the pH of the disinfectant is very important, and is preferably pH 9 to 12, particularly pH 9.1 to 10.8. Outside this range, the virus inactivation effect is poor.

In this embodiment, the pH of the disinfecting solution is adjusted using a buffer solution of sodium carbonate and sodium bicarbonate. By using sodium carbonate and sodium hydrogen carbonate, it is not necessary to use sodium hydroxide, a strong base similar to this, or a strong acid, and the safety of manufacturing workers can be improved. Moreover, the effect that the wiping performance with respect to a virus or protein stain | pollution | contamination improves by using sodium hydrogencarbonate is also acquired. Furthermore, it can be said that strong acid and strong base are highly reactive and difficult to store, and have a high environmental load. When sodium carbonate and sodium hydrogen carbonate are used, the storage stability is improved and the environmental load is low.

The contents of sodium carbonate and sodium hydrogen carbonate can be appropriately determined according to the target pH.

However, the content of sodium carbonate is preferably 0.01 to 0.3% by weight, more preferably 0.02 to 0.1% by weight, and 0.025 to 0.05% by weight. It is particularly preferable to do this. When the content of sodium carbonate is less than 0.01% by weight, the buffering ability of pH is remarkably lowered, and an effect can be expected for specific bacteria and sputum, but an effect cannot be expected for norovirus. There is knowledge. On the other hand, when the content of sodium carbonate exceeds 0.3% by weight, precipitation of components occurs when the water content decreases, leading to deterioration in the feeling of use.

The content of sodium hydrogen carbonate is preferably 0.08 to 0.8% by weight, more preferably 0.2 to 0.5% by weight, and 0.3 to 0.4% by weight. Is particularly preferred. When the content of sodium carbonate is less than 0.08% by weight, the buffering ability of pH is remarkably lowered, and an effect can be expected for specific bacteria and sputum, but an effect cannot be expected for norovirus. There is knowledge. On the other hand, when the content of sodium hydrogen carbonate exceeds 0.8% by weight, when moisture is reduced, precipitation of components occurs, leading to deterioration of the feeling of use.

In the disinfecting solution of this embodiment, alcohol is added to improve the antibacterial effect and the accompanying norovirus inactivation effect.

As the alcohol, for example, ethanol, propanol, butanol, isopropanol and the like can be used. As the glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, or the like can be used.

The alcohol content in the disinfectant can be determined as appropriate, but is preferably 40 to 80% by weight, more preferably 50 to 70% by weight. If the alcohol content is less than 40% by weight, the antibacterial effect is insufficient, and if it exceeds 80% by weight, there is no significant change in the antibacterial effect, and the cost effectiveness deteriorates. Propylene glycol is preferably 0.5 to 3% by weight, particularly preferably 1.00% by weight. The reason why propylene glycol is preferable in such a range is to improve the dispersion stability of antibacterial components other than the polyhexamethylene biguanide compound described later.

In the disinfecting solution of this embodiment, antibacterial components other than the polyhexamethylene biguanide compound can be contained.

Examples of other antibacterial components include benzalkonium chloride, cetylpyridinium chloride, phenoxyethanol, chlorohexidine gluconate, lauryl sulfate, and the like.

The content of other antibacterial components in the disinfectant may be appropriately determined according to the medicinal effect, but is preferably 0.01 to 0.3% by weight. It is preferable to set it as 05 weight%. When the content is less than 0.01% by weight, the antibacterial effect by the antibacterial component becomes insufficient, and when it exceeds 0.3% by weight, the antibacterial effect and its sustainability are not significantly changed, and the cost effectiveness is deteriorated. The reason why the above range is preferable for benzalkonium chloride is that bacteria such as Gram-positive and negative bacteria existing in the environment can be effectively removed.

The solvent used for the disinfectant of this embodiment is not particularly limited, but water is preferable.

In addition to the above, the disinfecting solution of the present embodiment may contain trace components such as a moisturizer such as aloe extract and glycine, and a preservative such as methylparaben, ethylparaben and propylparaben. The content of this trace component can be, for example, 0.1 to 0.3% by weight.

On the other hand, the content of the disinfecting solution of the present embodiment with respect to the base material can be determined as appropriate. For example, in the case of a non-woven base material, the content is preferably 200 to 400% by weight with respect to the absolute dry weight of the base material. If the content with respect to the substrate is less than 200% by weight, the liquid content is small and dirt is difficult to remove, and if it exceeds 400% by weight, the surface to be wiped becomes difficult to dry.

<About the base material>
Norovirus disinfecting article of the present embodiment, for example, a base material that can be used for wiping products is not particularly limited as long as it is made of a fiber assembly, and is mainly composed of paper made of pulp raw material, rayon, or PET. In addition to non-woven fabrics, woven fabrics and the like can be used. In addition to those that do not have water decomposability, those that have water decomposability can also be used.

The fiber constituting the substrate can be used regardless of whether it is natural, regenerated, or synthesized. In this embodiment, fibers containing at least hydrophilic fibers are preferable. Examples of hydrophilic fibers that can be used include natural fibers such as cotton and pulp, and regenerated fibers such as rayon and cupra. Among these fibers, rayon is particularly preferable. Rayon is rich in water absorption and is easy to handle, and a certain length of fiber can be obtained at low cost.

It is desirable that the hydrophilic fiber is blended in the base material at a content ratio of 50 to 70% by weight. When the content of the hydrophilic fiber is less than 50% by weight, sufficient flexibility and water retention cannot be given, and when it exceeds 70% by weight, the strength when wet is too low, and the tearing is caused. In addition to being easily generated, it becomes excessively stretched when taken out from the container in a pop-up manner.

In the base material of this embodiment, heat-fusible fibers can be used in addition to the hydrophilic fibers, and the fibers can be fused and bonded.

As the heat-fusible fiber, any fiber that melts by heating and exhibits adhesiveness to each other can be used.

The heat-fusible fiber may be a single fiber or a composite fiber in which two or more synthetic resins are combined. Specifically, polyolefin single fibers such as polyethylene, polypropylene and polyvinyl alcohol, polyethylene terephthalate / polyethylene, polyethylene terephthalate / polypropylene, polypropylene / polyethylene, polyethylene terephthalate-ethylene / propylene copolymer, low melting point polyester-polyester, etc. A core-sheath type composite fiber or an eccentric core-sheath type composite fiber whose sheath part is made of a relatively low melting point, or a part of each component made of polyethylene terephthalate / polypropylene, polyethylene terephthalate / nylon, polypropylene / polyethylene is the surface Splitting composite fiber exposed on the surface, or thermal splitting by heat shrinkage of one component of polyethylene terephthalate / ethylene-propylene copolymer Or the like can be used composite fibers.

In this case, a core-sheath type composite fiber is preferable when importance is attached to productivity and dimensional stability, and an eccentric type composite fiber is preferable if importance is attached to volume feeling. In addition, if importance is attached to flexibility, when a split type composite fiber or a heat split type composite fiber is used, each component can be easily divided into ultrafine fibers by high-pressure water flow treatment.

Such heat-fusible fibers are desirably blended at a content ratio of 10 to 30% by weight. When the heat-fusible fiber is less than 10% by weight, the wet strength cannot be secured, and the elongation becomes too large when the pop-up type is taken out from the container. On the other hand, if it exceeds 30% by weight, the texture becomes hard and the feel becomes rough, which is not preferable for this type of wet tissue.

In the base material of this embodiment, thermoplastic synthetic fibers can be mixed in addition to hydrophilic fibers and heat-fusible fibers.

As the thermoplastic fiber, various synthetic fibers exist, and among them, a polyester fiber is preferable. Polyolefin fibers such as polyethylene and polypropylene have poor confounding properties when subjected to high-pressure water flow treatment, and become inferior in fluffing and wet strength. Also, polyamide fibers such as nylon are hydrophilic and are not preferred.

The mixing amount of the thermoplastic fiber is preferably 40% by weight or less. When the content of the thermoplastic fiber exceeds 40% by weight, the water retention is impaired and the wet performance is remarkably lowered. By mixing thermoplastic fibers, the stiffness when wet is improved and a bulky nonwoven fabric can be obtained. Moreover, when a part is exposed to the nonwoven fabric surface, a sticky feeling is relieved even when wet due to hydrophobicity, and a dry feel is imparted.

Desirably, the thermoplastic fiber has thermal crimpability. In order to impart heat crimpability, a form of side-by-side type composite fiber in which synthetic resins having different heat shrinkage temperatures are bonded to thermoplastic fibers is adopted. For example, the melting point of the polyester fiber is 255 ° C. for polyethylene terephthalate and 215 ° C. for polybutylene terephthalate. As the low melting point resin to be bonded to this, a resin substantially the same as the melting point temperature of the above-mentioned heat-fusible fiber is used. It is better to do so.

In this embodiment, the shape of the base material is not particularly limited, and may have a thick block shape in addition to the sheet shape.

In the case of a sheet-like substrate, the basis weight of the substrate is preferably 20 to 80 g / m ² , particularly preferably 30 to 60 g / m ² . When the basis weight of the base material is less than 30 g / m ² , the ability to retain dirt becomes poor and it becomes difficult to provide unevenness as described later, and when it exceeds 60 g / m ² , the flexibility becomes poor.

The substrate may have a flat surface, but in order to improve the durability of the effect, unevenness is formed on one surface (one surface) or both surfaces (both surfaces) of the substrate. Is preferred. In this case, the dirt scraping effect is improved by the convex portion, and the dirt accommodation effect is improved by the concave portion. As a result, the effect of removing dirt on the surface to be wiped is improved, and the unevenness of the dirt becomes difficult to remain, so that volatilization of the disinfecting liquid is suppressed.

In a particularly preferred form, a large number of convex portions having a relatively high fiber density and concave portions having a relatively low fiber density are formed on the surface of the substrate. In this case, the scraping ability is improved by increasing the rigidity of the convex part, and the dirt accommodation capacity is improved by widening the fiber gap in the concave part. Moreover, since the base material which has such an unevenness | corrugation has a partial convex part with a high fiber density and a partial concave part with a low fiber density, it is structurally appropriate by the bendability in a recessed part with a low fiber density. Softness is imparted, and an appropriate texture is imparted by the uneven pattern on the surface.

In a more preferable base material, there are alternately ridges having a relatively high fiber density and a linear shape, and ridges having a relatively low fiber density and a linear shape. ing. The irregularities are preferably formed alternately, but the present invention is not limited to this, and irregularities can be formed irregularly. In addition, the irregularities are preferably formed in a linear shape, but can also be formed in a dotted shape.

When the linear irregularities are formed alternately and in parallel, the wiping effect by the convex portion and the accommodating effect by the concave portion are not exhibited at the time of wiping in the extending direction. Therefore, the linear pattern formed by the protruding line part and the recessed line part exhibits an intersecting pattern that intersects with the linear pattern formed by the other protruding line part and the recessed line part, for example, a herringbone pattern. Is preferred. In this case, no matter which direction is wiped off, dirt is pushed into the concave part due to the presence of the convex part, and finally, the dirt is scooped out by the convex part, so that it is wiped cleanly Can be done.

As the intersecting pattern of the linear pattern formed by the protruding line part and the recessed line part, it is only necessary that the linear pattern intersects with another linear pattern. In addition to the herringbone pattern, a lattice pattern, a rhombus pattern, etc. Various patterns can be used.

It is desirable that the number of lines of the linear pattern formed by the convex and concave portions is 3 to 9 / cm. When the number of lines is less than 3 / cm, the non-woven fabric tends to be flat, so that once captured dirt is easily transferred, and when the number of lines exceeds 9 / cm, Since the volume of the space formed by the recess is too small, a desired amount of dirt cannot be secured in the recess, which is undesirable.

The height (thickness) from the back surface (opposite surface) at the ridge is 300 to 800 μm, preferably 450 to 650 μm, and the height (thickness) from the back surface (opposite surface) at the ridge is It is desirable that the thickness is 100 to 500 μm, preferably 200 to 400 μm. From another point of view, the height difference between the ridge and the groove is preferably 50 to 300 μm, and preferably 75 to 150 μm. When the height difference is less than 50 μm, a large amount of capturing effect by the concave portions cannot be expected and a desired wiping amount cannot be secured. In addition, when the height difference exceeds 300 μm, the thickness of the base material increases as a result, and the flexibility and the touch feeling are impaired.

The base material having the unevenness described above can be manufactured, for example, as follows. That is, a fiber web comprising a hydrophilic fiber such as rayon, a heat-fusible fiber such as polyethylene or polypropylene, and a thermoplastic fiber such as polyester fiber on a wire mesh having a desired surface irregularity pattern to be applied to the substrate. And a high-pressure water stream is jetted from above the fiber web during conveyance so that the fibers are entangled. At this time, the fiber in the portion of the wire mesh where the wire is present is pushed to the both sides of the wire by the collision energy of the high-pressure water stream and moved to the opening side, so that the fiber portion of the mesh opening portion has a relatively high fiber density. While becoming higher, it becomes convex according to the shape of the opening, while the portion where the wire is present has a relatively low fiber density and a concave shape is formed along the wire.

The fibers are entangled with each other by the high-pressure water flow, and the entire fiber web is integrated. Thereafter, heat treatment is performed on the integrated fiber web at a temperature close to the melting point of the heat-fusible fiber, the fibers are bonded to each other by melting the heat-fusible fiber, and the thermoplastic fiber has heat crimpability. Bulkiness is provided by crimping.

The above manufacturing method is an application of the spunlace method, but other methods such as a wet method, a dry method, a spunbond method, a melt blow method, a needle punch method, and a stitch bond method are used to form irregularities. It can be applied to the production of substrates as much as possible.

In order to confirm the effect of the present invention, a norovirus inactivation experiment was conducted.
(Content of inactivation experiment)
Conventionally, when evaluating inactivation of norovirus, the amount of virus infecting cultured cells has generally been measured, and feline calicivirus and mouse norovirus have been substituted as viruses. However, in this example, the latest evaluation method using Norovirus-like particles is adopted. Therefore, the evaluation method is also described in detail below.

The outline of this evaluation method is that norovirus-like particles that are structurally and antigenically equivalent to norovirus are used as antigens, and the inactivation of norovirus is evaluated by quantifying the antibody bound to the norovirus-like particles. The antibody is quantified based on an ELISA (Enzyme-Linked Immunosorbent Assay) sandwich method.

In the ELISA sandwich method, a specimen is added to a microplate on which a primary antibody (capture antibody) is solid-phased to cause an antigen / antibody reaction, and an enzyme-labeled antibody is further added to cause an antigen / antibody reaction. By measuring the enzyme activity, the antigen in the sample is detected.

[Norovirus inactivation evaluation method]
First, a primary antibody (capture antibody) is adsorbed (immobilized) on the surface of each well of a microplate (96-well plate). Here, the primary antibody is an antibody used for capturing an antigen (norovirus-like particle).

Next, the diluted specimen is added dropwise to each well of the microplate on which the primary antibody is solid-phased in a predetermined order and at regular time intervals, and the microplate is stirred for several seconds with a plate mixer.

Then, the upper surface of the microplate is covered with a wrap, and the microplate is allowed to stand at 20 to 30 ° C. until the antigen is bound to the primary antibody (40 minutes).

Next, the reaction solution in each well of the microplate is aspirated and removed in the same order and at the same time interval as when the diluted specimen is added dropwise.

Then, wash solution is added dropwise to each well, and after stirring for several seconds with a plate mixer, the wash solution is removed by suction.

Then, the enzyme-labeled antibody solution is added dropwise to each well in the same order and at the same time interval as when the diluted specimen is added dropwise, and stirred for several seconds with a plate mixer.

Then, cover the upper surface of the microplate with a wrap, etc., further cover the whole with an aluminum foil to shield it from light, and let the microplate stand at 20-30 ° C. for 20 minutes.

Then, the substrate solution is added dropwise to each well in the same order and at the same time interval as when the diluted specimen is added dropwise, and stirred for several seconds with a plate mixer.

Then, cover the upper surface of the microplate with a wrap, etc., further cover the whole with aluminum foil etc. to shield it from light, and let the microplate stand at 20-30 ° C. for 40 minutes.

Next, the reaction stop solution is added dropwise to each well of the microplate in the same order and at the same time interval as when the diluted specimen is added dropwise.

Then, within 30 minutes after the addition of the reaction stop solution, the absorbance of the colored solution in each well is measured with an auto reader (wavelength 450 nm), and the residual amount of norovirus-like particles that are antigens is quantified. Simulate the activation effect.

<Conditions for implementation>
(antigen)
The norovirus-like particles attached to the Norovirus antigen kit NV-AD (Denka Seken Co., Ltd.) were used.

(Primary antibody)
Anti-NV-GI and GII monoclonal antibodies (mouse) were used.

(Sample preparation)
The sample was prepared as follows.
Contact an rNV-VLPs (recombinant Norovirus-like particles) dispersion with an equal amount of test specimen liquid, and take a 100 μL sample with a micropipette at any time, and add LP diluent to the sample. Use diluted samples.
Here, the rNV-VLPs dispersion is obtained by diluting rNV-VLPs with physiological saline (0.9 w / v%) and mixing for 10 minutes.
The test specimen solution is each chemical solution (Example and Comparative Example) to be tested. The composition of each chemical solution is shown in Table 1.

(Diluted sample)
The diluted specimen was obtained by preparing each drug solution according to the specimen preparation method described above.

(Enzyme-labeled antibody solution)
A solution containing peroxidase labeled anti-NV antigen polyclonal antibodies (rabbit) and (mouse) and peroxidase labeled anti-NV-GII monoclonal antibody (mouse) was used.

(Substrate solution)
A solution containing 3,3 ′, 5,5′-tetramethylbenzidine (TMB) and hydrogen peroxide (0.009 w / v%) was used.

(Reaction stop solution)
0.3 mol / L sulfuric acid was used.

(Disinfection articles and results)
An article for disinfection was obtained by impregnating and infiltrating a chemical solution into a wet wipe (rayon / PET, spunlace 34 g / m ² ).

Table 2 shows the results of the contact test with the chemical solution, and Table 3 shows the results of the wiping test. In addition, the result (remaining rate of virus) is shown by setting the start time (0 seconds) as 100% by weight (contact test) or 1 before wiping (wiping test).

(Discussion)
From the results of the contact test, it was found that even when sodium carbonate and sodium hydrogen carbonate were used instead of glycine buffer as a pH adjuster, a sufficient inactivation effect (0% after 30 seconds of contact) was obtained. Moreover, it turned out that the inactivation effect is equivalent even if it uses sodium carbonate and sodium hydrogencarbonate instead of a glycine buffer from the result of a wiping test.

The present invention can be used for disinfection of norovirus adhering to the skin or an article touched by the skin.

Claims

Containing 0.05 to 0.5% by weight of polyhexamethylene biguanide compound and 40 to 80% by weight of alcohol;
And containing sodium carbonate and sodium bicarbonate,
the pH is in the range of 9-12,
Norovirus disinfectant characterized by that.
The sodium carbonate content is 0.01-0.3 wt%,
The content of the sodium bicarbonate is 0.08 to 0.8% by weight,
The norovirus disinfecting solution according to claim 1.
The disinfecting solution according to claim 1 or claim 2 is impregnated in a substrate made of a fiber assembly.
Norovirus disinfecting article characterized by the above.