JP2006526994A - 方法 - Google Patents
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- JP2006526994A JP2006526994A JP2006516372A JP2006516372A JP2006526994A JP 2006526994 A JP2006526994 A JP 2006526994A JP 2006516372 A JP2006516372 A JP 2006516372A JP 2006516372 A JP2006516372 A JP 2006516372A JP 2006526994 A JP2006526994 A JP 2006526994A
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Treatments Of Macromolecular Shaped Articles (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polymerisation Methods In General (AREA)
Abstract
Description
i)単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させるが、前記単量体を付着させている間、前記単量体および/または前記表面を相互に関連させて動かすことで非均一プラズマ重合表面を生じさせ、そして
ii)前記プラズマ重合表面の少なくとも一部に結合体(binding entity)を導入することで前記結合体が非均一表面を形成するようにする、
ことを含んで成る。
i)単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させるが、前記単量体を付着させている間、前記単量体および/または前記表面を相互に関連させて動かすことで非均一プラズマ重合表面を生じさせ、
ii)前記プラズマ重合表面の少なくとも一部に1番目の結合体を導入することで前記結合体が非均一表面を形成するようにし、そして
iii)前記1番目の結合体と結合する2番目の結合体を前記1番目の結合体と接触させる、
ことを含んで成る。
i)空間的に離れて位置する少なくとも2つの単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させることで非均一プラズマ重合表面を生じさせる、
ことを含んで成る。
て同じ軸に沿って同じ方向にか或は別の軸に沿ってリガンド(B)の濃度が低くなるようにしてもよい。
i)本発明に従う基質を準備し、
ii)前記基質の表面を選別することで前記表面に結合した生体分子の結合特性を決定するが、前記結合特性は、それが前記表面に結合する位置で識別可能であり、
iii)前記結合特性を用いて生体分子の識別を行う、
ことを含んで成る。
al Chemistry B 101:9548−54、1997)のステンシルアプローチを用いたのでは、そのような表面を得るのは不可能である。当該基質の形態は単にプラズマパターンの最大解像度に影響を与える。
材料および方法
プラズマ重合の方法論がWO 01/31339に開示されており、これは引用することによって全体が本明細書に組み入れられる。
体流入口を数個有しかつプラズマを励起させる電極を有する。下方の室にプレシジョンXYZマニピュレーション(manipulation)ステージが入っており、これの上にパターン化を受けさせる基質を取り付ける。
プラズマ勾配の「書き込み」
異なる2種類の単量体化合物を用いて官能性勾配(functionality gradient)を付着させた。アリルアミンおよびアクリル酸をAldrich(UK)から入手して、それらに凍結−ポンプ−解凍サイクルを数回受けさせることで溶解していた気体を除去した。〜100マイクロンの穴が1個開いているマスクをマスクプレートに取り付けた後、1片のシリコンウエーハを基質として用い、これを持ち上げることで触れないように前記マスクにできるだけ近づけた(この上に記述したように)。最初に、アクリル酸単量体供給材料のみを用いてプラズマを励起させた。次に、単量体ガスの混合を変えると同時に前記マスクの下に位置するサンプルを直線的に動かした。それによって生じた最初の付着物は全体がアクリル酸プラズマ重合体で構成されていたが、後の付着物はアリルアミンとアクリル酸の混合物で構成されており、そして最後の付着部分は全体がアリルアミンで構成されていた。このように、この実験中に動かしたサンプルの範囲に渡って表面の組成がカルボン酸基が優位に占める領域からアミン基が優位に占める領域に滑らかに変化した。
n)にしておいてもよいか、或は書き込みを行う前の基質に局所的造形を刻み込んでおいてもよい。それによって表面上に三次元の官能構造物を単一工程で生じさせること(例えば底に沿って「掘った溝」にアミン官能基を付着させることなど)ができる。
〈実施例〉
プラズマ重合体表面の調製
持した。[この2種類の単量体の流量(cm3 stp分−1)の比率がそれらのモル比に相当する(挙動が理想的であると仮定して)]。
アミン基勾配へのビオチンの結合
酸基勾配上で細胞を培養
Claims (43)
- 基質の少なくとも1つの表面の少なくとも一部を準備する方法であって、
i)単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させ、かつ前記単量体を付着させている間、前記単量体および/または前記表面を相互に関連させて動かすことで非均一プラズマ重合表面を生じさせ、そして
ii)前記プラズマ重合表面の少なくとも一部に結合体を導入することで前記結合体が非均一表面を形成するようにする、
ことを含んで成る方法。 - 前記結合体を前記プラズマ重合表面の官能基と共有結合で相互作用させる請求項1記載の方法。
- 前記結合体を前記プラズマ重合表面の官能基と非共有結合で相互作用させる請求項1記載の方法。
- さらなる結合体を前記プラズマ重合表面に導入した前記結合体と相互作用させる請求項2または3記載の方法。
- 前記結合体が細胞、代謝産物、薬学的に活性のある薬剤;ホルモン、抗体、酵素、受容体を包含する蛋白質;DNA、RNA、蛋白質断片、ペプチド、ポリペプチドを包含する巨大分子;リガンド、プロテオグリカン、炭水化物、ヌクレオチド、オリゴヌクレオチド、毒性試薬および化学種から成る群から選択される請求項1記載の方法。
- i)単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させ、かつ前記単量体を付着させている間、前記単量体および/または前記表面を相互に関連させて動かすことで非均一プラズマ重合表面を生じさせ、
ii)前記プラズマ重合表面の少なくとも一部に1番目の結合体を導入することで前記結合体が非均一表面を形成するようにし、そして
iii)前記1番目の結合体と結合する2番目の結合体を前記1番目の結合体と接触させる、
段階を含んで成る請求項1記載の方法。 - i)空間的に離れて位置する少なくとも2つの単量体源から少なくとも1種のプラズマ単量体を前記表面に付着させることで非均一プラズマ重合表面を生じさせ、
ii)前記プラズマ重合表面の少なくとも一部に1番目の結合体を導入することで前記結合体が非均一表面を形成するようにし、そして
iii)前記1番目の結合体と結合する2番目の結合体を前記1番目の結合体と接触させる、
段階を含んで成る請求項1記載の方法。 - 前記単量体が揮発性アルコールである請求項1記載の方法。
- 前記単量体が揮発性酸である請求項1記載の方法。
- 前記単量体が揮発性アミンである請求項1記載の方法。
- 前記単量体が揮発性炭化水素である請求項1記載の方法。
- 前記単量体が揮発性フルオロカーボンである請求項1記載の方法。
- 前記単量体がエチレンオキサイド型分子である請求項1記載の方法。
- 前記単量体が揮発性シロキサンである請求項1記載の方法。
- 前記単量体がN−ビニルピロリドン、アリルアルコール、アクリル酸、オクタ−1,7−ジエン、アリルアミン、パーフルオロヘキサン、テトラエチレングリコールのモノアリルエーテルおよびヘキサメチルジシロキサン(HMDSO)から成る群から選択される請求項1記載の方法。
- 前記重合体を単一種の単量体から生じさせる請求項1記載の方法。
- 前記単量体が本質的にエチレン系不飽和有機化合物から成る請求項16記載の方法。
- 前記単量体が本質的に単一種のエチレン系不飽和有機化合物から成る請求項17記載の方法。
- 前記単量体がエチレンオキサイド型分子から成る請求項18記載の方法。
- 前記単量体が2種以上のエチレン系不飽和有機化合物の混合物から成る請求項17記載の方法。
- 前記化合物が炭素原子を20個以下の数で含有するアルケン、カルボン酸、アルコールおよびアミンから成る群から選択される請求項17記載の方法。
- 前記重合体が共重合体である請求項1記載の方法。
- 前記共重合体が少なくとも1種の有機単量体と少なくとも1種の炭化水素を含んで成る請求項22記載の方法。
- 前記単量体が少なくとも6.6x10−2ミリバールの蒸気圧を示す重合性単量体である請求項1記載の方法。
- 前記単量体1種または2種以上を前記表面に空間的に離れて位置する点の状態で付着させる請求項1記載の方法。
- 前記単量体1種または2種以上を前記表面に軌道または線の状態で付着させる請求項1記載の方法。
- 前記線、軌道または点の化学的組成および/または官能性を長さ方向に沿って非均一にする請求項25または26記載の方法。
- 前記線、軌道または点の化学的組成および/または官能性を高さ方向に非均一にする請求項1記載の方法。
- 前記表面に非結合表面がもたらされるようにエチレン−オキサイド型単量体の重合体で構成されていてプラズマが付着しない領域を含める請求項1記載の方法。
- 請求項1記載の方法で得ることができる表面を含んで成る基質。
- プラズマ重合表面を含んで成っていて前記表面が少なくとも1番目と2番目の重合体領域を含んで成りかつ前記1番目の領域の組成と前記2番目の領域の組成が異なる基質であって、少なくとも一方の領域に1番目の結合体が結合していて前記1番目の結合体に2番目の結合体が結合することを特徴とする基質。
- 該基質がガラス、プラスチック、ニトロセルロース、ポリフッ化ビニリデン(PVdF)、ポリカーボネート、ポリ(メタアクリル酸メチル)、ナイロン、金属、セラミック、石英、複合構造体およびケイ素ウエーハから成る群から選択される請求項30または31記載の基質。
- 前記プラスチックがポリエチレンテレフタレート、高密度ポリエチレン、低密度ポリエチレン、ポリ塩化ビニル、ポリプロピレンおよびポリスチレンから成る群から選択される請求項30または31記載の基質。
- 請求項30または31記載の基質を含んで成る製品。
- 該製品が検定用製品の一部である請求項34記載の製品。
- 前記検定用製品がマイクロアレイである請求項35記載の製品。
- 前記検定用製品がマイクロタイタープレートである請求項35記載の製品。
- 該製品がマイクロ流体工学素子または一部を含んで成る請求項34記載の製品。
- 請求項30または31記載の基質を含んで成る細胞培養装置であって、前記結合体が少なくとも1種の細胞が増殖し得る表面を与えている細胞培養装置。
- 該装置が検定用製品の一部である請求項39記載の細胞培養装置。
- 生物学的分子を分離する時に請求項30または31記載の基質を用いる使用。
- 前記生物学的分子が細胞、代謝産物、薬学的に活性のある薬剤;ホルモン、抗体、酵素、受容体を包含する蛋白質;DNA、RNA、蛋白質、ペプチド、ポリペプチドを包含する巨大分子;リガンド、プロテオグリカン、炭水化物、ヌクレオチド、オリゴヌクレオチド、毒性試薬および化学種から成る群から選択される請求項41記載の使用。
- 生物学的分子を選別する方法であって、
i)請求項30または31記載の基質を準備し、
ii)前記基質の表面を選別することで前記表面に結合した生物学的分子の結合特性を決定するが、前記結合特性は、それが前記表面に結合する位置で識別可能であり、そして
iii)前記結合特性を用いて生物学的分子の識別を行う、
段階を含んで成る方法。
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US8802603B2 (en) | 2010-06-17 | 2014-08-12 | Becton, Dickinson And Company | Medical components having coated surfaces exhibiting low friction and low reactivity |
US9541480B2 (en) | 2011-06-29 | 2017-01-10 | Academia Sinica | Capture, purification, and release of biological substances using a surface coating |
US9828521B2 (en) | 2012-09-28 | 2017-11-28 | Ut-Battelle, Llc | Durable superhydrophobic coatings |
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