JP2006519590A - タバコ属由来のシトクロムp450遺伝子のクローニング - Google Patents

タバコ属由来のシトクロムp450遺伝子のクローニング Download PDF

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JP2006519590A
JP2006519590A JP2005501429A JP2005501429A JP2006519590A JP 2006519590 A JP2006519590 A JP 2006519590A JP 2005501429 A JP2005501429 A JP 2005501429A JP 2005501429 A JP2005501429 A JP 2005501429A JP 2006519590 A JP2006519590 A JP 2006519590A
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amino acid
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nicotiana
acid sequence
nucleic acid
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JP2006519590A5 (enExample
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シュー,ドンメイ
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ユーエス スモークレス タバコ カンパニー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2005501429A 2002-10-16 2003-10-16 タバコ属由来のシトクロムp450遺伝子のクローニング Withdrawn JP2006519590A (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41893302P 2002-10-16 2002-10-16
US48536803P 2003-07-08 2003-07-08
US50398903P 2003-09-18 2003-09-18
PCT/US2003/032722 WO2004035745A2 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Related Child Applications (1)

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JP2010019897A Division JP2010142241A (ja) 2002-10-16 2010-02-01 タバコ属由来のシトクロムp450遺伝子のクローニング

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JP2006519590A true JP2006519590A (ja) 2006-08-31
JP2006519590A5 JP2006519590A5 (enExample) 2006-11-30

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JP2005501429A Withdrawn JP2006519590A (ja) 2002-10-16 2003-10-16 タバコ属由来のシトクロムp450遺伝子のクローニング
JP2010019897A Pending JP2010142241A (ja) 2002-10-16 2010-02-01 タバコ属由来のシトクロムp450遺伝子のクローニング

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EP (3) EP2336305A3 (enExample)
JP (2) JP2006519590A (enExample)
KR (1) KR20050052541A (enExample)
AP (1) AP2005003286A0 (enExample)
AU (1) AU2003301431A1 (enExample)
BR (1) BR0315388A (enExample)
CA (1) CA2501631A1 (enExample)
MX (1) MXPA05004016A (enExample)
WO (1) WO2004035745A2 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508821A (ja) * 2003-10-16 2007-04-12 ユーエス スモークレス タバコ カンパニー タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング
JP2007512003A (ja) * 2003-10-16 2007-05-17 ユーエス スモークレス タバコ カンパニー タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング
WO2008117887A1 (ja) * 2007-03-28 2008-10-02 Japan Tobacco Inc. 燃焼煙中のカルボニル類含量が低減されたタバコ属植物及びその作製法
JP2010512751A (ja) * 2006-12-15 2010-04-30 ユーエス スモークレス タバコ カンパニー 低減されたニコチンデメチラーゼ活性を有するタバコ植物

Families Citing this family (32)

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US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
KR20040091124A (ko) * 2002-03-12 2004-10-27 유.에스.스모크리스 토바코 컴퍼니 니코티아나 사이토크롬 p450 유전자의 클로닝
EP2330191A1 (en) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from nicotiana
ATE554161T1 (de) * 2003-10-16 2012-05-15 Us Smokeless Tobacco Co Nutzung eines cytochrom-p450-gen aus nicotiana
EP2933328A1 (en) * 2004-04-29 2015-10-21 U.S. Smokeless Tobacco Company LLC Nicotiana nucleic acid molecules and uses thereof
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
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JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Nicotiana核酸分子およびその用途
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EP2087123B1 (en) * 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
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PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
BR112013021515A2 (pt) 2011-02-28 2018-06-12 Univ North Carolina State plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90
KR20140034160A (ko) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 담배 근친교배 식물체 albex1f 및 albex1ms
WO2014110363A1 (en) 2013-01-11 2014-07-17 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
CN103718957B (zh) * 2013-12-12 2016-03-02 湖北省烟草科研所 通过未受精胚珠培养雄性不育烟草单倍体的方法
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN106659232A (zh) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 烟草近交和杂种植物以及由其制得的烟草产品
EP3113603A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508821A (ja) * 2003-10-16 2007-04-12 ユーエス スモークレス タバコ カンパニー タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング
JP2007512003A (ja) * 2003-10-16 2007-05-17 ユーエス スモークレス タバコ カンパニー タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング
JP2010512751A (ja) * 2006-12-15 2010-04-30 ユーエス スモークレス タバコ カンパニー 低減されたニコチンデメチラーゼ活性を有するタバコ植物
JP2013172738A (ja) * 2006-12-15 2013-09-05 Us Smokeless Tobacco Co Llc 低減されたニコチンデメチラーゼ活性を有するタバコ植物
WO2008117887A1 (ja) * 2007-03-28 2008-10-02 Japan Tobacco Inc. 燃焼煙中のカルボニル類含量が低減されたタバコ属植物及びその作製法

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Publication number Publication date
EP1581633A4 (en) 2008-10-15
EP1581633A2 (en) 2005-10-05
AU2003301431A1 (en) 2004-05-04
EP2336305A3 (en) 2011-10-05
EP2336305A2 (en) 2011-06-22
JP2010142241A (ja) 2010-07-01
WO2004035745A8 (en) 2007-12-06
CA2501631A1 (en) 2004-04-29
AU2003301431A8 (en) 2008-02-28
MXPA05004016A (es) 2006-01-27
BR0315388A (pt) 2005-08-23
EP2204444A1 (en) 2010-07-07
AP2005003286A0 (en) 2005-06-30
KR20050052541A (ko) 2005-06-02
WO2004035745A2 (en) 2004-04-29

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