AU2003301431A1 - Cloning of cytochrome p450 genes from nicotiana - Google Patents

Cloning of cytochrome p450 genes from nicotiana

Info

Publication number
AU2003301431A1
AU2003301431A1 AU2003301431A AU2003301431A AU2003301431A1 AU 2003301431 A1 AU2003301431 A1 AU 2003301431A1 AU 2003301431 A AU2003301431 A AU 2003301431A AU 2003301431 A AU2003301431 A AU 2003301431A AU 2003301431 A1 AU2003301431 A1 AU 2003301431A1
Authority
AU
Australia
Prior art keywords
nicotiana
cytochrome
cloning
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003301431A
Other languages
English (en)
Other versions
AU2003301431A8 (en
Inventor
Dongmei Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Smokeless Tobacco Co LLC
Original Assignee
US Smokeless Tobacco Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Smokeless Tobacco Co LLC filed Critical US Smokeless Tobacco Co LLC
Publication of AU2003301431A1 publication Critical patent/AU2003301431A1/en
Priority to AU2004282588A priority Critical patent/AU2004282588B2/en
Priority to AU2004282576A priority patent/AU2004282576B2/en
Publication of AU2003301431A8 publication Critical patent/AU2003301431A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2003301431A 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana Abandoned AU2003301431A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2004282588A AU2004282588B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
AU2004282576A AU2004282576B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US41893302P 2002-10-16 2002-10-16
US60/418,933 2002-10-16
US48536803P 2003-07-08 2003-07-08
US60/485,368 2003-07-08
US50398903P 2003-09-18 2003-09-18
US60/503,989 2003-09-18
PCT/US2003/032722 WO2004035745A2 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Related Child Applications (2)

Application Number Title Priority Date Filing Date
AU2004282588A Division AU2004282588B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
AU2004282576A Division AU2004282576B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana

Publications (2)

Publication Number Publication Date
AU2003301431A1 true AU2003301431A1 (en) 2004-05-04
AU2003301431A8 AU2003301431A8 (en) 2008-02-28

Family

ID=32110829

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003301431A Abandoned AU2003301431A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Country Status (9)

Country Link
EP (3) EP2336305A3 (enExample)
JP (2) JP2006519590A (enExample)
KR (1) KR20050052541A (enExample)
AP (1) AP2005003286A0 (enExample)
AU (1) AU2003301431A1 (enExample)
BR (1) BR0315388A (enExample)
CA (1) CA2501631A1 (enExample)
MX (1) MXPA05004016A (enExample)
WO (1) WO2004035745A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
KR20040091124A (ko) * 2002-03-12 2004-10-27 유.에스.스모크리스 토바코 컴퍼니 니코티아나 사이토크롬 ｐ450 유전자의 클로닝
EP2330191A1 (en) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from nicotiana
JP4519137B2 (ja) * 2003-10-16 2010-08-04 ユーエス スモークレス タバコ カンパニー タバコ属（Ｎｉｃｏｔｉａｎａ）からのチトクロムｐ４５０遺伝子のクローニング
ATE554161T1 (de) * 2003-10-16 2012-05-15 Us Smokeless Tobacco Co Nutzung eines cytochrom-p450-gen aus nicotiana
CN102321641A (zh) * 2003-10-16 2012-01-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
EP2933328A1 (en) * 2004-04-29 2015-10-21 U.S. Smokeless Tobacco Company LLC Nicotiana nucleic acid molecules and uses thereof
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
ES2373881T3 (es) * 2004-04-29 2012-02-09 U.S. Smokeless Tobacco Company Llc Clon genómico de nicotina desmetilasa de tabaco y usos del mismo.
WO2005111217A2 (en) * 2004-04-29 2005-11-24 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Ｎｉｃｏｔｉａｎａ核酸分子およびその用途
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
US20060156430A1 (en) * 2005-01-13 2006-07-13 Mcgonigle Brian Novel cytochrome P450 monooxygenase
EP1851317B1 (en) 2005-02-23 2011-10-26 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EP1940868A2 (en) 2005-10-07 2008-07-09 Cornell University Polynucleotides encoding phenylpropanoid pathway enzymes in coffee
EP2087123B1 (en) * 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
JP2010136624A (ja) * 2007-03-28 2010-06-24 Japan Tobacco Inc 燃焼煙中のカルボニル類含量が低減されたタバコ属植物及びその作製法
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
BR112013021515A2 (pt) 2011-02-28 2018-06-12 Univ North Carolina State plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90
KR20140034160A (ko) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 담배 근친교배 식물체 albex1f 및 albex1ms
WO2014110363A1 (en) 2013-01-11 2014-07-17 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
CN103718957B (zh) * 2013-12-12 2016-03-02 湖北省烟草科研所 通过未受精胚珠培养雄性不育烟草单倍体的方法
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN106659232A (zh) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 烟草近交和杂种植物以及由其制得的烟草产品
EP3113603A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof

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Also Published As

Publication number Publication date
EP1581633A4 (en) 2008-10-15
JP2006519590A (ja) 2006-08-31
EP1581633A2 (en) 2005-10-05
EP2336305A3 (en) 2011-10-05
EP2336305A2 (en) 2011-06-22
JP2010142241A (ja) 2010-07-01
WO2004035745A8 (en) 2007-12-06
CA2501631A1 (en) 2004-04-29
AU2003301431A8 (en) 2008-02-28
MXPA05004016A (es) 2006-01-27
BR0315388A (pt) 2005-08-23
EP2204444A1 (en) 2010-07-07
AP2005003286A0 (en) 2005-06-30
KR20050052541A (ko) 2005-06-02
WO2004035745A2 (en) 2004-04-29

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MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase