JP2006282598A - Melanogenesis inhibitor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a melanogenesis inhibitor having both of the active oxygen-eliminating action and the tyrosinase activity-inhibiting action and capable of multilaterally inhibiting melanogenesis. <P>SOLUTION: The present invention provides a melanogenesis inhibitor containing an extract of Hibiscusrosa-sinensis, the melanogenesis inhibitor which is a bleaching agent, the melanogenesis inhibitor which is an external preparation, the melanogenesis inhibitor which is an medicine for internal use, the melanogenesis inhibitor which is an agent for oral cavity, the melanogenesis inhibitor which is a food and the melanogenesis inhibitor which is a beverage. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a melanin production inhibitor. More specifically, the present invention relates to a melanin production inhibitor that effectively prevents pigmentation such as spots and freckles by suppressing the production of melanin from multiple angles.

  Conventionally, it is known that the deposition of pigments such as spots and freckles is caused by the production and accumulation of melanin in skin cells. The production process of melanin has not been fully elucidated, but the following process has become clear as one of the main production processes: (1) Skin is exposed to ultraviolet rays. Reactive oxygen is generated in the epidermal cells of (1), (2) cells are damaged by active oxygen, and various signal transmitters are generated and released. (3) Tyrosinase production promotion and activity by various signal transmitters Causes melanin to form. Therefore, in order to suppress the production of melanin and keep the skin white, it is considered as effective means to eliminate active oxygen on the skin, suppress the production of tyrosinase, or inhibit tyrosinase activity. Yes.

  So far, active oxygen scavengers such as ascorbic acid and tyrosinase activity inhibitors such as kojic acid and arbutin have been proposed as active ingredients of whitening agents (Patent Document 1, etc.). However, the substances proposed as the active ingredients of conventional whitening agents only act on part of the melanin production process, and melanin is produced by other processes, so its melanin production inhibitory effect is satisfactory. It wasn't. Furthermore, the conventional whitening agent components are not satisfactory from the viewpoint of the sustained effect. Against the background of such conventional technology, development of a melanin production inhibitor that effectively suppresses pigment deposition by acting in a multifaceted manner on the melanin production process, and a melanin production inhibitor excellent in sustainability are desired. It was.

On the other hand, the hibiscus extract itself is used in combination with various proliferative effects such as fibroblast proliferation effect (Patent Document 2), anti-inflammatory effect (Patent Document 3, Patent Document 4), and other physiologically active ingredients. It has been reported that a hair function enhancing effect is exhibited (Patent Document 5), and that a specific hibiscus extract exhibits an active oxygen scavenging effect and a melanin production suppressing effect (Patent Document 6). However, since there are various varieties of hibiscus, the physiologically active components contained in each hibiscus vary depending on the variety, and it is impossible to uniformly discuss the presence or absence of the physiologically active component. That is, the effect exhibited by the already reported hibiscus extract is not inherent in all hibiscuses, but is caused by specific hibiscuses.
JP-A-1-85907 Japanese Patent Laid-Open No. 10-36279 JP-A-57-99517 JP-A-9-87188 JP 2000-7545 A JP 2000-95663 A

  An object of the present invention is to provide a melanin production inhibitor capable of solving the conventional problems as described above and acting on the melanin production process in a multifaceted manner and effectively suppressing pigment deposition. It is. Specifically, it is intended to provide a melanin production inhibitor capable of effectively suppressing melanin production by effectively having active oxygen scavenging action and tyrosinase activity inhibition action, and effectively preventing pigmentation such as spots and freckles. It is the purpose.

  The present inventors have been diligently studied to solve the above-mentioned problems. As a result, the extract of Bushroom, which is a natural product-derived ingredient and known as a food material, has an excellent active oxygen scavenging action and a tyrosinase activity inhibitory action. It has been found that the production of melanin can be multifaceted. Therefore, from such knowledge, the present inventors have convinced that a melanin production inhibitor containing a Bushroom extract as an active ingredient is effective as a whitening agent. Furthermore, from its excellent active oxygen scavenging action, it was convinced that it was effective as an internal preparation, particularly a food. The present invention has been completed based on such findings.

That is, the present invention is the following melanin production inhibitors:
Item 1. A melanin production inhibitor comprising a Bushroom extract.
Item 2. Item 2. The melanin production inhibitor according to Item 1, which is used as a whitening agent.
Item 3. Item 3. The melanin production inhibitor according to Item 1 or 2, which is an external preparation.
Item 4. Item 3. The melanin production inhibitor according to Item 1 or 2, which is an internal preparation.
Item 5. Item 3. The melanin production inhibitor according to Item 1 or 2, which is an oral preparation.
Item 6. Item 3. The melanin production inhibitor according to Item 1 or 2, which is a food or drink.

  In addition, the melanin production inhibitor as used in the field of this invention has the effect which suppresses the production | generation of melanin based on an active oxygen scavenging action or a tyrosinase activity inhibitory action. Moreover, the whitening agent as used in the field of this invention has a whitening effect based on this melanin production inhibitory action.

Hereinafter, the present invention will be described in more detail.
(A) Melanin production inhibitor The melanin production inhibitor of the present invention is characterized in that it contains a Bushroom extract that has been used as a food raw material and has been confirmed to be safe as an active ingredient. .

Bushsoge (scientific name: Hibiscus rosa-sinensis ) used in the present invention is a plant species belonging to the family Malvaceae and genus Hibiscus , and its origin is China, and it is native to temperate regions in Japan. It has been known. The bushwort used in the present invention may be a wild species or an improved variety.

  The extract used as the active ingredient of the present invention can be prepared by extracting from Bushroom. There are no particular restrictions on the extraction site of Bushroom, and examples include the whole plant of the plant, or a part of the plant such as flowers, buds, leaves, stems, branches, branch tips, roots, rhizomes, and seeds. . Among these, a flower can be mentioned preferably.

  The Bushroom extract used in the present invention is a cold water, hot water or an organic solvent, or water and an organic material obtained by subjecting the above extraction object as it is or after drying, chopping, crushing, pressing, boiling or fermentation treatment. It can be obtained by extraction with an extraction solvent such as a solvent mixture. As an organic solvent used for this extraction, for example, methanol, ethanol, isopropanol, propylene glycol, 1,3-butylene glycol, acetone, ethyl acetate, chloroform, pentane, hexane, heptane, etc., alone or in combination of two or more kinds are used. Can be mentioned. Among the above extraction solvents, preferably, lower alcohols such as methanol, ethanol, isopropanol, or a mixture of water and lower alcohols can be used.

  The method for extracting the Bushroom extract used in the present invention is not particularly limited, and can be performed according to a conventional method. As an example, the flowers of Bushow are pulverized, and 3 to 5 parts by weight of 30% ethanol aqueous solution is added to 1 part by weight of the extraction object, followed by extraction for 2-3 days with stirring at room temperature, followed by filtration. The method of removing solid content can be mentioned.

  The Bushroom extract thus obtained is a liquid dissolved in an extraction solvent, and the extract can be used as it is in the present invention, but preferably the organic solvent is removed by distillation under reduced pressure or the like. It is. Further, if necessary, it can be used as a dried product by removing moisture by subjecting it to a drying treatment such as reduced pressure drying or freeze drying.

  Moreover, as a Bushroom extract, what was obtained commercially can also be used simply.

  The melanin production inhibitor of the present invention may be composed of Bushroom extract itself, and contains other ingredients such as a pharmaceutically or food hygienically acceptable carrier or additive as an active ingredient. It may be. The type and blending amount of the carrier or additive can be appropriately selected and adjusted according to the dosage form of the melanin production inhibitor or the object to be used, as long as the effects of the present invention are not impaired. Further, the shape of the melanin production inhibitor of the present invention is not particularly limited, and may be liquid, emulsion, cream, powder, granule according to the dosage form, form, use, etc. of the applied product. , Pills, ointments and the like.

  The melanin production inhibitor of the present invention can effectively suppress the production of melanin and effectively suppress the deposition of pigment, and therefore can be applied as a whitening agent.

  In prescribing the melanin production inhibitor of the present invention, it can be an external preparation, an internal preparation, or an oral preparation.

  In prescribing the melanin production inhibitor of the present invention as an external preparation, if necessary, inorganic pigments, ultraviolet absorbers, whitening ingredients, tyrosinase activity inhibitors, melanin pigment reducing agents, surfactants, cell activators, Additives such as inflammatory agents, antibacterial agents, humectants, fragrances, and coloring agents may be added as appropriate. Although it does not restrict | limit especially as an external preparation which can apply the melanin production inhibitor of this invention, For example, external pharmaceutical products, external quasi-drugs (skin external preparation etc.), basic cosmetics (skin lotion, milky lotion, cream, ointment) , Lotions, oils, packs, etc.), makeup cosmetics (foundation, lipstick, blusher, mascara, etc.), other skin cosmetics (facial cleansers, skin cleansers, massage agents, cleansing agents, etc.) or bath preparations Can be mentioned.

  When prescribing the melanin production inhibitor of the present invention as an external preparation, the amount to be added to the external preparation varies depending on the compounding target, dosage form, expected effect, etc., and cannot be defined uniformly. In 100% by weight of the external preparation, the range of Bushoujou extract is 0.01 to 10% by weight, preferably 0.1 to 3% by weight, more preferably 0.1 to 1% by weight.

  In prescribing the melanin production inhibitor of the present invention as an internal preparation, additives such as a binder, a disintegrant, a lubricant, a wetting agent, a buffer, an excipient, an extender, a preservative, and a fragrance are added. It is good also as oral preparations, such as a powder, a tablet, a granule, a capsule, or a syrup, by mix | blending. Moreover, when prescribing the melanin production inhibitor of the present invention as an internal preparation, other pharmaceutically active ingredients can be further blended.

  When prescribing the melanin production inhibitor of the present invention as an internal preparation, the compounding amount in the internal preparation varies depending on the administration subject, dosage form, administration method, etc., and cannot be uniformly defined. In 100% by weight of the internal preparation, there can be mentioned a range in which the Bushou extract is 0.01 to 100% by weight, preferably 0.1 to 50% by weight, more preferably 1 to 30% by weight. .

  In addition, the dosage of the internal preparation of the present invention varies depending on the dosage form, administration subject, administration method, expected effect, etc., and cannot be defined uniformly. The product has a dry weight of 0.01 to 50 g / day, preferably 0.1 to 20 g / day, and more preferably about 1 to 10 g / day.

  In prescribing the melanin production inhibitor of the present invention as an oral preparation, if necessary, an adhesive, a refreshing agent, a binder, a sweetener, a flavoring agent, a disintegrating agent, a lubricant, a coloring agent, a sustained release adjustment. Additives such as an agent, a surfactant, a solubilizer, a wetting agent, and a pH adjuster may be added as appropriate. The oral preparation to which the melanin production inhibitor of the present invention can be applied is not particularly limited, and examples thereof include dentifrice such as toothpaste, mouthwash, mouth rinse, oral pasta, troche, gum massage cream and the like. be able to. The form is not particularly limited, and it can be prepared in various forms such as a solution, emulsion, ointment, sol, gel, paste, tablet, powder, spray, sheet, and film.

  When prescribing the melanin production inhibitor of the present invention as an oral preparation, the amount to be added to the oral preparation varies depending on the compounding target, dosage form, expected effect, etc., and cannot be defined uniformly. Usually, in 100% by weight of the oral preparation, the extract of Bushou is 0.01 to 10% by weight, preferably 0.1 to 3% by weight, more preferably 0.1 to 1% by weight. It is.

  The manufacturing method of an oral preparation is not specifically limited, According to a well-known method, it can manufacture suitably. The application method is not particularly limited, and an appropriate amount may be applied once to several times a day to the affected area or the entire oral cavity. The oral preparation of the present invention has an excellent active oxygen scavenging action, tyrosinase activity inhibitory action, and melanin production inhibitory action based on the inclusion of the above-mentioned Bushroom extract, and removes pigments deposited on gums In addition, an excellent whitening effect can be achieved.

  Furthermore, the melanin production inhibitor of the present invention can be prepared as a food, or can be prepared as a beverage, alone or in combination as a component.

  In preparing the melanin production inhibitor of the present invention as a food, it is not particularly limited. For example, it can be prepared in the form of powder, tablet, capsule, syrup, jelly and the like. Various foods such as confectionery (gum, candy, cookies, rice crackers, biscuits, etc.), marine products (boiled rice, bamboo rings, etc.), seasonings (sauce, mayonnaise, sprinkles, spices, dressings, etc.), bread, noodles, cereals, etc. It can also be blended as a component.

  In preparing the melanin production inhibitor of the present invention as a beverage, although not particularly limited, for example, carbonated beverages, soft drinks, milk beverages, coffee beverages, mineral water, alcoholic beverages, fruit juice beverages, teas, nutritional beverages, etc. It can be blended as a component of a beverage.

  In addition, the said foodstuff and drink may belong to categories, such as health food, food for sick people, food for specified health, and dietary supplement.

  When the melanin production inhibitor of the present invention is prepared as a food or beverage, the blending amount in the food or beverage varies depending on the form, expected effect, etc., and cannot be uniformly defined. In such a case, the range is such that, in 100% by weight of the food, the Bushou extract is, for example, 0.01 to 100% by weight, preferably 0.1 to 50% by weight, and more preferably 1 to 30% by weight. In the case of a beverage, the Bushou extract is in a dry weight of, for example, 0.01 to 50% by weight, preferably 0.1 to 50% by weight, more preferably 1 to 30% by weight in 100% by weight of the beverage Can be mentioned.

  In addition, the daily intake of the food or beverage is not particularly limited, but from the viewpoint of the melanin production inhibitory effect, for example, in the case of an adult, the Bushou extract is usually 0.01 to 50 g / dry weight. The amount that is about 1 to 20 g / day, more preferably about 1 to 10 g / day, can be used as a guide.

In addition, as shown in Test Example 1 which will be described later, the Bushroom extract used in the present invention has an excellent action of scavenging active oxygen (superoxide scavenging action), and as an active ingredient of an active oxygen scavenger. It can also be used. That is, the present invention includes the following aspects of the invention.
(1) A reactive oxygen scavenger characterized by containing a Bushroom extract.
(2) The active oxygen scavenger according to (1), which is an external preparation.
(3) The active oxygen scavenger according to (1), which is an internal preparation.
(4) The active oxygen scavenger according to (1), which is an oral preparation.
(5) The active oxygen scavenger according to (1), which is a food.
(6) The active oxygen scavenger according to (1), which is a beverage.

In addition, as shown in Test Example 2 to be described later, the Bushroom extract used in the present invention has an action of inhibiting the enzyme activity even when tyrosinase is produced, and should be used as a tyrosinase activity inhibitor. You can also. That is, the present invention includes the following aspects of the invention.
(11) A tyrosinase activity inhibitor characterized by containing a Bushroom extract.
(12) The tyrosinase activity inhibitor according to (11), which is an external preparation.
(13) The tyrosinase activity inhibitor according to (11), which is an internal preparation.
(14) The tyrosinase activity inhibitor according to (11), which is an oral preparation.
(15) The tyrosinase activity inhibitor according to (11), which is a food.
(16) The tyrosinase activity inhibitor according to (11), which is a beverage.

  The melanin production inhibitor comprising the Bushroom extract of the present invention as an active ingredient has an excellent active oxygen scavenging action, and also has a tyrosinase activity inhibitory action. Can be effectively suppressed, and can be applied as a whitening agent having an excellent whitening effect.

  In addition, the Bushroom extract used in the present invention has been conventionally used as a raw material for food and has been confirmed to be safe. Therefore, it can be formulated as an internal preparation, an oral preparation or an external preparation. It can also be used as a component of an oral composition such as a beverage or toothpaste. Therefore, according to the melanin production inhibitor of the present invention, it is possible to provide a wide variety of means for achieving whitening by suppressing the production of melanin.

  EXAMPLES Hereinafter, although a manufacture example, a test example, and an Example are given and this invention is demonstrated in more detail, this invention is not limited at all by the following Examples.

Production example

100 g of dried flowers of Bussouge (Fussia mulberry) (scientific name: Hibiscus rosa-sinensis L.) were pulverized with a mill, and 500 mL of 30% ethanol aqueous solution was added thereto, followed by extraction treatment at room temperature for 3 days. Next, the solid content was removed by filtration, and the filtrate was concentrated under reduced pressure, and then lyophilized to obtain 15 g of Bushroom extract.

  In addition, unless otherwise indicated, the test examples and examples shown below were performed using the Bushroom extract obtained by the above method.

Test example 1

Evaluation of the active oxygen scavenging effect In order to evaluate the active oxygen scavenging effect of the Bushroom extract, Superoxide dismutase (SOD) analysis kit-WST (manufactured by Dojindo Molecular Technologies) was used. Was measured. A specific method is described below. In addition, about the solution etc. which are not specifically described with the following method, what was equipped with the said measurement kit or the thing adjusted according to the usage method of this kit was used.

First, 20 μL of a Bushroom extract solution (hereinafter referred to as a target sample solution) whose concentration was adjusted so that the final well concentration would be 0.0033 to 0.8 wt% was added to each well in a 96-well microplate. Next, 20 μL of tetrazolium salt solution was added to each well and mixed well. Thereafter, 20 μL of the enzyme solution was added to each well and immediately incubated at 37 ° C. for 20 minutes, and then the absorbance at 450 nm was measured with a microplate reader. As a control, the following conditions were also measured in the same manner. The superoxide elimination rate (%) was calculated from the measured absorbance value based on the following formula.
・ Blank: No enzyme solution added (added diluted solution of enzyme solution instead) ・ Target sample solution added ・ Control 1: Enzyme solution added ・ No target sample solution added (diluted target sample solution added instead)
-Control 2: No enzyme solution added (added diluted solution of enzyme solution instead)-No target sample solution added (added diluted solution of target sample solution instead)

なお、比較として、活性酸素消去能を有することが知られている物質であるアスコルビン酸、及びチロシナーゼ阻害活性に基づいて美白剤として使用されているコウジ酸、アルブチン、さらに同じハビスカス属のロゼル（Hibiscus sabdariffa L.）およびムクゲ（Hibiscus syriacus L.)の抽出物を、ブッソウゲ抽出物の代わりに用いて、同様に測定を行った。

For comparison, ascorbic acid, a substance known to have active oxygen scavenging ability, and kojic acid, arbutin, which are used as whitening agents based on tyrosinase inhibitory activity, and the same Habiscus roselle ( Hibiscus Sabdariffa L. and extract of Hibiscus syriacus L. were used in place of the Bushou extract, and the same measurement was performed.

  The obtained results are shown in FIG. In FIG. 1, the superoxide elimination rate (%) in each addition density | concentration of a bussouge extract, a roselle extract, a mugwort extract, ascorbic acid, kojic acid, and arbutin is shown, respectively.

  As a result, Bushroom extract has superoxide scavenging ability comparable to ascorbic acid, which has been conventionally known as a substance having high superoxide scavenging ability, and other hibiscus species (Rozel extract, Mucuge extract). ) Was confirmed to be more effective.

Test example 2

Evaluation of Tyrosinase Activity Inhibitory Effect In order to evaluate the activity inhibitory effect of tyrosinase in the extract of Bushroom, the following test was conducted.
Mouse-derived B16 melanoma cells (Dainippon Pharmaceutical B16F0) were seeded in a 96-well microplate at 5 × 10 ⁴ cells / well and cultured overnight to fix the cells. Next, the culture solution on the culture plate was removed with an aspirator, and PBS (phosphate buffered saline) containing 1% Triton X-100 was added at 50 μl / well to disrupt the cell membrane structure to obtain a crude tyrosinase solution. Next, 50 μl / well of a Bushroom extract solution diluted with PBS (referred to as a target sample in the following formula) was added so that the final concentration in the well would be 10 mg / ml. Thereafter, 50 μL of 0.1% dopa solution diluted with PBS was added to each well, immediately incubated at 37 ° C. for 4 hours, and absorbance at 405 nm was measured using a microplate reader. As a control, the following conditions were also measured in the same manner. Based on the measured absorbance value, the tyrosinase activity inhibition rate (%) was calculated based on the following formula.
・ Blank: B16 melanoma cell non-seeded ・ Bussouge extract solution added corresponding to target sample ・ Blank: B16 melanoma cell non-seeded ・ Busso extract extract added Addition / Control 1: B16 melanoma cell seeding / Bususu extract extract solution not added (PBS added instead)
・ Control 2: B16 melanoma cell non-seeding ・ Bususu extract extract solution not added (PBS added instead)

なお、比較として、同じハビスカス属のムクゲ（Hibiscus syriacus L.)の抽出物をブッソウゲ抽出物の代わりに用いて、同様に測定を行った。得られた結果を 図２に示す。 図２には、ブッソウゲ抽出物とムクゲ抽出物のチロシナーゼ活性阻害率（％）を示している。

For comparison, the same measurement was carried out using the same extract of Hibiscus syriacus L. instead of the extract of Bushouge. The obtained results are shown in FIG. FIG. 2 shows the tyrosinase activity inhibition rate (%) of the Bushroom extract and Mungweed extract.

  From this result, it was confirmed that the Bushroom extract has a higher tyrosinase activity inhibitory action than the extract of Mucuge which has already been known to have a whitening effect.

Test example 3

Melanin production inhibitory effect From the above results, it has been clarified that the extract of Bushroom has an active oxygen scavenging action and a tyrosinase activity inhibitory action involved in the synthesis of melanin. In this test, the production inhibitory effect on the melanin itself was evaluated. The test was conducted according to the following method.

Mouse-derived B16 melanoma cells (RCB 0557) were seeded in a 6-well plate at about 10 ⁵ cells / well and cultured overnight to fix the cells. Next, a Bushroom extract solution (referred to as a target sample in the following formula) diluted with PBS so that the final concentration in the well becomes 0.2 to 2% is added, and after culturing for 4 days, 0.25% The cells were detached using a trypsin solution, and the cells were collected by centrifugation. After visually confirming the color difference of the collected cells, the membrane structure of the cells was destroyed with 1N NaOH to elute melanin, and the absorbance at 405 nm was measured. In addition, as a control, the measurement was performed in the same manner for a system to which no Bushroom extract was added (hereinafter referred to as an additive-free sample in the following formula). Furthermore, in order to correct the error at the time of preparing the solution, the amount of protein in the sample was measured using a quantification kit, corrected with the amount of unit protein, and the melanin production rate (%) was calculated based on the following formula.

なお、比較として、同じハビスカス属のムクゲ（Hibiscus syriacus L.)の抽出物をブッソウゲ抽出物の代わりに用いて、同様に測定を行った。得られた結果を 図３に示す。図３には、ブッソウゲ抽出物とムクゲ抽出物の各添加濃度におけるメラニン生成率（％）を示す。

For comparison, the same measurement was carried out using the same extract of Hibiscus syriacus L. instead of the extract of Bushouge. The obtained results are shown in FIG. In FIG. 3, the melanin production rate (%) in each addition density | concentration of a Bushroom extract and a Mokuge extract is shown.

  As a result, it was confirmed that the addition of Bushroom extract significantly suppressed the production of melanin, and also confirmed that it has a higher melanin production-inhibiting effect than the extract of Mukugei, which already has a known whitening effect. It was.

Formulation example

<Formulation Example 1> Lotion (weight%)
Bushroom extract 1.0
Ascorbic acid phosphate magnesium 3.0
Polypropylene glycol 2.0
Oxybenzone 3.0
P-Hydroxybenzoate ester 0.5
Citric acid appropriate amount Fragrance appropriate amount
Purified water balance
Total 100.0.

<Formulation example 2> Cosmetic cream (wt%)
Bushroom extract 0.5
Ascorbic acid monostearate 3.0
Cysteine 0.1
Stearyl alcohol 5.0
Stearic acid 8.0
Squalane 10.0
Self-emulsifying glyceryl monostearate 3.0
Polyoxyethylene cetyl ether (20E.0) 1.0
Propylene glycol 5.0
Potassium hydroxide 0.3
Perfume appropriate amount Preservative appropriate amount
Purified water balance
Total 100.0.

<Formulation example 3> Emulsion (wt%)
Bushroom extract 1.0
Hibiscus acid monoethyl 1.0
Squalane 8.0
Vaseline 2.0
Beeslow 0.5
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20E.0) 1.2
Carboxyvinyl polymer 0.2
Propylene glycol 0.5
Potassium hydroxide 0.1
Ethanol 7.0
Perfume appropriate amount Preservative appropriate amount
Purified water balance
Total 100.0.

<Formulation example 4> Bread (% by weight)
The raw materials were blended at the blending ratio shown in the following table, and bread was produced according to a conventional method.
Flour 51.0
Sugar 3.0
Salt 1.0
Bushroom extract 0.5
Nonfat dry milk 1.0
Oils and fats 3.1
East Food 0.1
East 1.0
Purified water balance
Total 100.0.

<Prescription Example 5> Tablet (% by weight)
Bushroom extract 10
Ascorbic acid phosphate magnesium 10
Placenta extract 10
Collagen 20
Crystalline cellulose 20
Dextrin 14
Glycerin fatty acid ester 6
Excipient 10
Total 100.

<Formulation example 6> Drink (weight%)
Bushroom extract 1.0
Fructose glucose 20.0
Citric acid 0.5
Trisodium citrate 0.1
Ascorbic acid 0.2
Perfume appropriate amount
Purified water balance
Total 100.0.

<Prescription Example 7> Toothpaste No.1 (% by weight)
Dicalcium phosphate 30.0
Glycerin 10.0
Sorbitol 20.0
Sodium carboxymethylcellulose 1.0
Sodium lauryl sulfate 1.5
Carrageenan 0.5
Saccharin sodium 0.1
Fragrance 1.0
Sodium benzoate 0.3
Bushroom extract 0.1
Purified water balance
Total 100.0.

<Prescription Example 8> Toothpaste No.2 (% by weight)
Sodium lauryl sulfate 0.80
Lauric acid diethanolamide 1.00
Propylene glycol 3.00
60% Sorbit liquid 35.00
Methylparaben 0.20
Butylparaben 0.01
Saccharin sodium 0.15
Gelatin 0.30
Fragrance 0.60
Sodium alginate 0.90
Sodium benzoate 0.10
Aluminum hydroxide 45.00
Sodium monofluorate 0.76
Bushroom extract 0.05
Purified water balance
Total 100.0.

<Prescription Example 9> Mouthwash (wt%)
Ethanol 10.00
Glycerin 5.00
Citric acid 0.01
Sodium citrate 0.10
Polyoxyethylene hydrogenated castor oil 0.50
Methyl paraoxybenzoate 0.10
Fragrance 0.20
Bushroom extract 0.1
Purified water balance
Total 100.0.

<Prescription Example 10> Oral pasta (% by weight)
Liquid paraffin 13.00
Cetanol 10.00
Glycerin 25.00
Sorbitan monopalmitate 0.60
Polyoxyethylene sorbitan monostearate 5.00
Sodium lauryl sulfate 0.10
Benzotonium chloride 0.10
Methyl salicylate 0.10
Saccharin 0.20
Fragrance 0.25
Bushroom extract 0.50
Purified water balance
Total 100.00.

It is a figure which shows the relationship between the addition density | concentration of a Bushroom extract and a superoxide elimination rate (%) (Test Example 1). For comparison, the relationship between the additive concentration and the superoxide elimination rate (%) is similarly shown for roselle extract, mugweed extract, kojic acid, arbutin, and ascorbic acid. It is a figure which shows the inhibition rate (%) of tyrosinase activity when the final density | concentration of a Bushroom extract is 10 mg / ml (Test Example 2). For comparison, the rate of inhibition of tyrosinase activity (%) at the same concentration is also shown for the extract of Mukuge. The melanin production rate (%) in each addition density | concentration of a Bushroom extract is shown (Test Example 3). For comparison, the melanin production rate (%) at each added concentration is also shown for the Mukuge extract.

Claims (1)

A melanin production inhibitor comprising a Bushroom extract.

Images