JP2006276663A - Microscope - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microscope which allows an observation image having a magnification different from that of a visual observation image to be satisfactorily acquired by an image pickup device simultaneously with visual observation. <P>SOLUTION: An inverted microscope body 1 has a revolver 5 below a stage 2. The revolver 5 is capable of holding a plurality of objective lenses 4 and is supported turnably by a revolver stand 6. The inverted microscope body 1 is provided with an optical path switching prism 13 below the revolver stand 6. The optical path switching prism 13 divides an observation optical path extending through an objective lens 4 disposed in an observation position, into an optical path for camera observation extending to a side through a camera port 17 and an optical path for visual observation which is turned back by a downward extending mirror 23 and extends upward obliquely. An imaging lens 15 for visual observation is disposed on the optical path for visual observation, and an imaging lens 16 for camera observation is disposed on the optical path for camera observation. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a microscope having a visual observation optical path and a camera observation optical path.

  FIG. 8 is a side view of a conventional inverted microscope, FIG. 9 is a front view thereof, and FIG. 10 is a top view thereof. As shown in FIGS. 8, 9, and 10, the inverted microscope body 1 has a stage 2, and a specimen 3 is placed on the stage 2. The inverted microscope body 1 has a revolver 5 below the stage 2. The revolver 5 can hold a plurality of objective lenses 4 and is rotatably supported by a revolver holding base 6. The plurality of objective lenses 4 held by the revolver 5 are selectively disposed at an observation position below the sample 3 and used. The objective lens 4 arranged at the observation position is switched by the rotation of the revolver 5.

  The revolver holding base 6 is supported so as to be linearly movable up and down, and is connected to the rotary focusing knob 7 via a rack and pinion mechanism (not shown) provided inside the inverted microscope body 1. The rotational movement of the rotary focusing knob 7 is converted into a linear movement of the revolver holding base 6 by a rack and pinion mechanism.

  The lamp house 8 has a built-in light source 9 and is fixed to the inverted microscope body 1 through a light projection tube 10. The inverted microscope main body 1 includes a mirror cassette 11 for guiding light from the lamp house 8 to the objective lens 4 arranged at the observation position below the revolver 5. The mirror cassette 11 has a mirror 12 that is detachably fixed. The light emitted from the light source 9 enters the mirror cassette 11 through the projection tube 10 and is reflected toward the objective lens 4 arranged at the observation position by the mirror 12 in the mirror cassette 11.

  The inverted microscope main body 1 includes an imaging lens 14 and an optical path switching prism 13 below the mirror cassette 11. The imaging lens 14 has a focal length of 180 mm. The optical path switching prism 13 includes a prism 13a that transmits 100% of light from the specimen 3 on the optical axis O of the objective lens 4 disposed at the observation position, and 100% or partially camera port of the light from the specimen 3. A prism 13b that reflects toward the lens 17 can be selectively disposed.

  The optical path switching prism 13 divides the observation optical path extending through the objective lens 4 disposed at the observation position into a camera observation optical path and a visual observation optical path. The optical path for camera observation extends laterally from the optical path switching prism 13 through the camera port 17. The optical path for visual observation extends downward from the optical path switching prism 13, is folded back by the mirror 23, and extends obliquely upward.

  On the optical path for visual observation that extends obliquely upward, an eyepiece 24 that enables visual observation of an image formed by the visual observation imaging lens 15 is disposed. The eyepiece 24 is detachably attached to the inverted microscope body 1.

  A camera adapter 27 is attached to the camera port 17, and a TV camera 19 and a camera 28 are attached to the camera adapter 27. The camera adapter 27 includes an optical path dividing half mirror 18 that divides the optical path for camera observation into two, and the TV camera 19 and the camera 28 are positioned on the divided optical path for camera observation. The optical path dividing half mirror 18 reflects 50% of light and transmits 50%. The TV camera 19 is a general-purpose high-sensitivity camera, and has a size of, for example, 150 mm × 90 mm × 100 mm. The camera 28 is a general-purpose analog camera and has a size of, for example, 80 mm × 30 mm × 30 mm.

  The optical path splitting half mirror 18 is disposed at a position of 135 mm along the optical path from the imaging lens 14. The imaging surface 28 a of the camera 28 is disposed at a position 45 mm from the optical path splitting half mirror 18 on the reflected optical path of the optical path splitting half mirror 18, that is, at the imaging position of the imaging lens 14. The imaging surface 19 a of the TV camera 19 is disposed at a position 45 mm from the optical path dividing half mirror 18 on the transmission optical path of the optical path dividing half mirror 18, that is, at the imaging position of the imaging lens 14.

  Part of the light exiting the sample 3 passes through the objective lens 4 to become a parallel light beam, passes through the mirror cassette 11, passes through the imaging lens 14, and enters the optical path switching prism 13. The light transmitted through the optical path switching prism 13 is reflected by the mirror 23 and then enters the eyepiece lens 24. The imaging lens 14 forms an incident light, and the image can be observed through the eyepiece 24. The light reflected by the optical path switching prism 13 passes through the camera port 17 to the camera adapter 27 and is split by the optical path splitting half mirror 18. The light reflected by the optical path dividing half mirror 18 forms an image on the imaging surface 28 a, and the image is acquired by the camera 28. Further, the light transmitted through the optical path dividing half mirror 18 forms an image on the imaging surface 19 a, and the image is acquired by the TV camera 19.

  Japanese Patent Application Laid-Open No. 6-250093 discloses a sample detection apparatus that can simultaneously obtain images of samples having different magnifications. In this sample detection apparatus, the light from the sample passes through the objective lens and then is divided into three camera observation optical paths by a beam splitter. The three camera observation optical paths are a high magnification observation optical path, a low magnification observation optical path, and a medium magnification observation optical path. A convex lens is provided in the high magnification observation optical path, and a concave lens is provided in the low magnification observation optical path. In addition, no variable magnification lens is provided in the optical path for medium magnification observation.

Japanese Patent Laid-Open No. 2002-31758 discloses a microscope capable of observing a wide field of view at a low magnification and observing a high resolution at a high magnification without requiring an operation of switching an objective lens. This microscope has an objective lens that collects light from the specimen, an optical path dividing element that divides the optical path from the objective lens into an optical path for visual observation and an optical path for camera observation, and a magnification conversion that is arranged on the optical path for camera observation. And an optical system.
JP-A-6-250093 JP 2002-31758 A

  In microscope observation, a technique is often used in which a cell to be viewed at a high magnification is magnified and recorded by a TV camera while searching for the position of the sample by visual observation in a wide field of view at a low magnification.

  However, in the microscope shown in FIGS. 8 to 10, the magnification is switched by attaching a low-magnification objective lens and a high-magnification objective lens to the microscope revolver, and rotating the revolver to switch between the low-magnification objective lens and the microscope. This is done by switching a high-magnification objective lens and placing it on the optical path. For this reason, time loss occurs when the magnification is changed. That is, observation with an imaging device cannot be performed simultaneously with visual observation. Further, since there is a considerable misalignment when the objective lens is switched, the observation position of the sample is deviated.

  Therefore, instead of changing the magnification by switching the objective lens, a method of increasing the magnification by providing a zoom lens in the optical path for camera observation can be considered, but this increases the number of lenses, which causes image degradation and loss of light quantity. End up.

  In the sample detection apparatus disclosed in Japanese Patent Laid-Open No. 6-250093, light from a specimen is collected by an objective lens, and the light passing through the objective lens converges to form an image. This apparatus is substantially the same as the optical path for camera observation shown in FIG. 10 regardless of the difference that the imaging lens is built in the objective lens. Since the zooming of each camera uses a zooming lens, image degradation and loss of light amount occur. Further, this apparatus configuration cannot be realized for a focal length of about 180 mm to 200 mm, and therefore cannot be applied to a microscope having a visual observation optical path. The reason is as follows.

  In a microscope equipped with an optical path for visual observation, an imaging lens having a focal length of about 180 mm to 200 mm is usually used. This is because if the focal length of the imaging lens is very long, the eye point position becomes high and the operability becomes poor. Further, if an image forming lens having a very long focal length is used and an eye point position is to be kept at an optimum position, it is necessary to fold the lens repeatedly with a prism or the like, which causes image deterioration. For these reasons, the focal length of the imaging lens is optimally about 180 mm to 200 mm.

  The microscope disclosed in Japanese Patent Application Laid-Open No. 2002-31758 has a magnification conversion optical system in the optical path for camera observation, so that image deterioration and light amount loss occur due to an increase in the number of lenses.

  The present invention has been made in consideration of such a situation, and the purpose of the present invention is to make it possible to obtain an observation image having a magnification different from that of the visual observation image at the same time as the visual observation with the imaging device. Is to provide.

  The microscope of the present invention includes an objective lens that creates a parallel light beam from light from an object, an optical path dividing unit that divides an observation optical path extending through the objective lens into an optical path for camera observation and an optical path for visual observation, and an optical path for visual observation An imaging lens for visual observation, a camera observation imaging lens arranged on the optical path for camera observation, and an eyepiece that enables visual observation of an image formed by the imaging lens for visual observation. I have.

  ADVANTAGE OF THE INVENTION According to this invention, the microscope which makes it possible to acquire favorably the observation image of the magnification different from a visual observation image simultaneously with visual observation with an imaging device is provided.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

[First embodiment]
FIG. 1 is a side view of an inverted microscope according to the first embodiment of the present invention, FIG. 2 is a front view thereof, and FIG. 3 is a top view thereof.

  As shown in FIGS. 1, 2, and 3, in the inverted microscope of the present embodiment, the inverted microscope body 1 is held horizontally on a surface plate 36. The upper surface of the surface plate 36 is processed with high flatness, and screw holes are provided at equal intervals. The inverted microscope main body 1 has a stage 2, and a specimen 3 is placed on the stage 2. The inverted microscope body 1 has a revolver 5 below the stage 2. The revolver 5 can hold a plurality of objective lenses 4 and is rotatably supported by a revolver holding base 6. The plurality of objective lenses 4 held by the revolver 5 are selectively disposed at an observation position below the specimen 3 by the rotation of the revolver 5 and used. The objective lens 4 arranged at the observation position creates a parallel light beam from the light emitted from the sample 3.

  The revolver holding base 6 is supported so as to be linearly movable up and down, and is connected to the rotary focusing knob 7 via a rack and pinion mechanism (not shown) provided inside the inverted microscope body 1. The rotational movement of the rotary focusing knob 7 is converted into a linear movement of the revolver holding base 6 by a rack and pinion mechanism.

  The lamp house 8 has a built-in light source 9 and is fixed to the inverted microscope body 1 through a light projection tube 10. The inverted microscope main body 1 includes a mirror cassette 11 for guiding light from the lamp house 8 to the objective lens 4 arranged at the observation position below the revolver 5. The mirror cassette 11 has a mirror 12 that is detachably fixed. The light emitted from the light source 9 enters the mirror cassette 11 through the projection tube 10 and is reflected toward the objective lens 4 arranged at the observation position by the mirror 12 in the mirror cassette 11.

  The inverted microscope main body 1 includes an optical path switching prism 13 below the mirror cassette 11. The optical path switching prism 13 includes a prism 13a that transmits 100% of light from the specimen 3 on the optical axis O of the objective lens 4 disposed at the observation position, and 100% or partially camera port of the light from the specimen 3. A prism 13b that reflects toward the lens 17 can be selectively disposed.

  The optical path switching prism 13 divides the observation optical path extending through the objective lens 4 disposed at the observation position into a camera observation optical path and a visual observation optical path. The optical path for camera observation extends laterally from the optical path switching prism 13 through the camera port 17. The optical path for visual observation extends downward from the optical path switching prism 13, is folded back by the mirror 23, and extends obliquely upward.

  The inverted microscope body 1 further includes a visual observation imaging lens 15 on the visual observation optical path, and a camera observation imaging lens 16 on the camera observation optical path. The visual observation imaging lens 15 is positioned below the optical path switching prism 13 and has a focal length of 180 mm. The imaging lens 16 for camera observation is located on the side of the optical path switching prism 13 and has a focal length of 360 mm.

  On the optical path for visual observation that extends obliquely upward, an eyepiece 24 that enables visual observation of an image formed by the visual observation imaging lens 15 is disposed. The eyepiece 24 is detachably attached to the inverted microscope body 1.

  On the camera observation optical path, three optical path dividing half mirrors 18a, 18b and 18c are arranged outside the inverted microscope body 1. The optical path splitting half mirrors 18a, 18b, and 18c are fixed to the surface plate 36 by brackets 37a, 37b, and 37c, respectively, so that the positions thereof can be adjusted. The optical path dividing half mirrors 18a, 18b, and 18c reflect 50% of light emitted from the inverted microscope body 1 through the camera port 17 and transmit 50% thereof. The three optical path splitting half mirrors 18a, 18b, and 18c split the camera observation optical path extending from the optical path switching prism 13 into four.

  TV cameras 19, 20, 21, and 22 are arranged on the four divided optical paths for camera observation, respectively. The TV cameras 19, 20, 21, and 22 are fixed to the surface plate 36 by brackets 38a, 38b, 38c, and 38d, respectively, so that the position can be adjusted. The TV cameras 19, 20, 21, and 22 are general-purpose high-sensitivity cameras, and are not limited thereto, but have a size of 150 mm × 90 mm × 100 mm, for example.

  The optical path dividing half mirror 18a is disposed at a position 120 mm from the imaging lens 16 for camera observation, and partially reflects light emitted from the camera port 17 by 90 degrees. The optical path splitting half mirror 18b is disposed at a position 50 mm from the optical path splitting half mirror 18a, and partially reflects the light transmitted through the optical path splitting half mirror 18a by 90 degrees. The optical path dividing half mirror 18c is disposed at a position 50 mm from the optical path dividing half mirror 18b, and partially reflects the light transmitted through the optical path dividing half mirror 18b by 90 degrees. Two adjacent reflection directions of the optical path dividing half mirrors 18a, 18b and 18c are set to be opposite to each other so that the cameras on the reflected optical paths of the optical path dividing half mirrors 18a, 18b and 18c do not interfere with each other.

  The imaging surface 19a provided inside the TV camera 19 is disposed at a position 240 mm from the optical path dividing half mirror 18a on the reflected optical path of the optical path dividing half mirror 18a. This is the imaging position of the imaging lens 16 for camera observation. The imaging surface 20a provided inside the TV camera 20 is disposed at a position 190 mm from the optical path splitting half mirror 18b on the reflected optical path of the optical path splitting half mirror 18b. This is the imaging position of the imaging lens 16 for camera observation. The imaging surface 21a provided inside the TV camera 21 is disposed at a position 140 mm from the optical path dividing half mirror 18c on the reflected optical path of the optical path dividing half mirror 18c. This is the imaging position of the imaging lens 16 for camera observation. The imaging surface 22a provided inside the TV camera 22 is disposed at a position 140 mm from the optical path dividing half mirror 18c on the transmitted optical path of the optical path dividing half mirror 18c. This is the imaging position of the imaging lens 16 for camera observation.

  Part of the light exiting the sample 3 passes through the objective lens 4 to become a parallel light beam, passes through the mirror cassette 11 and enters the optical path switching prism 13. The light transmitted through the optical path switching prism 13 passes through the visual observation imaging lens 15, is reflected by the mirror 23, and then enters the eyepiece lens 24. The visual observation imaging lens 15 forms an incident light, and the image can be observed through the eyepiece 24.

  The light reflected by the optical path switching prism 13 passes through the camera observation imaging lens 16 and is emitted from the camera port 17 to the outside of the inverted microscope body 1. The light emitted from the camera port 17 is divided by the optical path dividing half mirror 18 a, and the reflected light forms an image on the imaging surface 19 a, and the image is acquired by the TV camera 19. The light transmitted through the optical path splitting half mirror 18a is split by the optical path splitting half mirror 18b, reflected and imaged on the imaging surface 20a, and the image is acquired by the TV camera 20. The light transmitted through the optical path splitting half mirror 18b is split by the optical path splitting half mirror 18c, the reflected light is imaged on the imaging surface 21a, the image is acquired by the TV camera 21, and the transmitted light is captured on the imaging surface. An image is formed on 22 a, and the image is acquired by the TV camera 22.

  A generally well-known relational expression between the focal length and the magnification is shown in Expression (1).

Magnification M = focal length of imaging lens / magnification of objective lens (1)
According to equation (1), if the magnification of the objective lens is fixed and the focal length of the imaging lens is changed to twice, the magnification is also doubled.

  As a result, the magnification of the image formed by the camera observation imaging lens 16 having a focal length of 360 mm and acquired by the camera is the image formed by the visual observation imaging lens 15 having a focal length of 180 mm and acquired by the eyepiece. Twice as much. That is, low-magnification visual observation with a wide field of view and high-magnification TV camera observation can be performed without switching the objective lens 4.

  Further, in the inverted microscope shown in FIGS. 8 to 10, since the focal length of the imaging lens 14 is as short as 180 mm, the number of cameras that can be arranged on the camera observation optical path is at most two. On the other hand, in the inverted microscope of this embodiment, the camera observation imaging lens 16 is provided on the camera observation optical path separately from the visual observation imaging lens 15 on the visual observation optical path, and the focal length is 360 mm. Therefore, the camera observation optical path can be divided into three or more optical paths by a half mirror or the like. For this reason, it is possible to install three or more TV cameras. Simultaneous observation with three or more TV cameras is also possible.

  In this embodiment, a high-sensitivity camera having a general size is used as the TV camera, and four TV cameras are installed so that the TV cameras and the TV camera and the microscope main body do not interfere with each other. If a TV camera is used, it is possible to install more cameras.

(Modification of the first embodiment)
The inverted microscope of this modification has a configuration in which the optical path dividing half mirrors 18a, 18b, and 18c in the inverted microscope shown in FIGS. 1 to 3 are replaced with dichroic mirrors that combine optical path division and wavelength separation. .

  The first dichroic mirror applied as an alternative to the optical path dividing half mirror 18a has the wavelength characteristics shown in FIG. The second dichroic mirror applied as an alternative to the optical path dividing half mirror 18b has the wavelength characteristics shown in FIG. 5, and the third dichroic mirror applied as an alternative to the optical path dividing half mirror 18c is shown in FIG. It has the wavelength characteristics shown.

  As can be seen from FIG. 4, the first dichroic mirror has a low transmittance for light with a wavelength of 0 to 450 nm and a high transmittance for light with a wavelength of 451 nm or more. As can be seen from FIG. 5, the second dichroic mirror has a low transmittance for light with a wavelength of 0 to 505 nm and a high transmittance for light with a wavelength of 506 nm or more. As can be seen from FIG. 6, the third dichroic mirror has a low transmittance for light with a wavelength of 0 to 600 nm and a high transmittance for light with a wavelength of 600 nm or more.

  Part of the light exiting the sample 3 passes through the objective lens 4 to become a parallel light beam, passes through the mirror cassette 11 and enters the optical path switching prism 13. The light transmitted through the optical path switching prism 13 passes through the visual observation imaging lens 15, is reflected by the mirror 23, and then enters the eyepiece lens 24. The visual observation imaging lens 15 forms an incident light, and the image can be observed through the eyepiece 24.

  The light reflected by the optical path switching prism 13 passes through the camera observation imaging lens 16 and is emitted from the camera port 17 to the outside of the inverted microscope body 1. The light emitted from the camera port 17 is selectively reflected by the first dichroic mirror at a wavelength of 0 to 450 nm, and the reflected light is imaged on the imaging surface 19 a, and the image is acquired by the TV camera 19. Is done. The light having a wavelength of 451 nm or more transmitted through the first dichroic mirror is selectively reflected by the second dichroic mirror, and the light having a wavelength of 505 nm or less is reflected and imaged on the imaging surface 20a. Acquired by the camera 20. The light having a wavelength of 506 nm or more transmitted through the second dichroic mirror is selectively reflected by the third dichroic mirror with the light having a wavelength of 600 nm or less, and the reflected light forms an image on the imaging surface 21a. Acquired by the TV camera 21. In addition, light having a wavelength of 600 nm or more transmitted through the third dichroic mirror forms an image on the imaging surface 22 a, and the image is acquired by the TV camera 22.

  In this modification, it is possible to observe light of different wavelengths with different cameras. For example, a cell is stained with a fluorescent dye that emits fluorescence at a wavelength of 440 nm, a microtubule is stained with a fluorescent dye that emits light at a wavelength of 480 nm, a mitochondria is stained with a fluorescent dye that emits light at a wavelength of 540 nm, and is emitted at a wavelength of 700 nm. Assume that actin is stained with a fluorescent dye. In this case, the TV camera 19 can observe the nucleus, the TV camera 20 can observe the microtubules, the TV camera 21 can observe the mitochondria, and the TV camera 22 can observe the actin simultaneously. That is, different parts of cells can be observed simultaneously with different cameras.

Second Embodiment FIG. 7 is a top view of an inverted microscope according to a second embodiment of the present invention. 7, members indicated by the same reference numerals as those shown in FIG. 1, FIG. 2, and FIG. 3 are similar members, and detailed description thereof is omitted.

  As shown in FIG. 7, the microscope of the present embodiment includes four camera observation imaging lenses 16a, 16b, 16c, and 16d instead of the camera observation imaging lens 16 in the first embodiment. Yes. The camera observation imaging lens 16 a is disposed on the optical path between the optical path dividing half mirror 18 a and the TV camera 19. The camera observation imaging lens 16b is on the optical path between the optical path dividing half mirror 18b and the TV camera 20, and the camera observation imaging lens 16c is on the optical path between the optical path dividing half mirror 18c and the TV camera 21. The imaging lens 16d for camera observation is disposed on the optical path between the optical path dividing half mirror 18c and the TV camera 22.

  The imaging lenses 16a, 16b, 16c, and 16d for camera observation form images on the imaging surfaces 19a, 20a, 21a, and 22a of the corresponding TV cameras 19, 20, 21, and 22 according to their focal lengths. Placed in. In FIG. 7, the imaging lenses for camera observation 16a, 16b, 16c, and 16d are depicted as having the same focal length, but may have different focal lengths. That is, the focal lengths of the camera observation imaging lenses 16a, 16b, 16c, and 16d may be changed as appropriate. The focal lengths of the camera observation imaging lenses 16a, 16b, 16c, and 16d may be set shorter or longer than the focal length of the visual observation imaging lens 15. Further, an optical path with a long focal length and a short optical path may be mixed, or only a part of the optical paths may have the same focal length.

  The magnification of the images acquired by the TV cameras 19, 20, 21, and 22 is determined according to the focal lengths of the corresponding camera observation imaging lenses 16a, 16b, 16c, and 16d according to the above-described equation (1). Therefore, the TV cameras 19, 20, 21 and 22 can acquire images with different magnifications.

  Thus, in addition to the advantage of the inverted microscope of the first embodiment, the inverted microscope of the present embodiment has the advantage that the TV cameras 19, 20, 21, and 22 can acquire images with different magnifications. In this embodiment, since one camera observation imaging lens is provided for one camera, it is possible to easily install more cameras.

  Also, the same modification as the modification of the first embodiment can be applied to this embodiment. That is, also in the inverted microscope of the present embodiment, the optical path dividing half mirrors 18a, 18b, and 18c may be replaced with dichroic mirrors that perform both optical path division and wavelength separation.

  In each of the embodiments, the case where the number of imaging lenses for camera observation is one for each optical path has been described. However, the imaging lens for an arbitrary optical path is configured by two or more lenses, and is arranged before and after the optical path dividing member 18. You may arrange.

  Further, by configuring an arbitrary imaging lens to be detachable with respect to the optical path, it is possible to improve the system performance, for example, by exchanging with a different optical element.

  An imaging lens with a long focal length is arranged before the optical path is divided, and an imaging lens is also arranged on each of the divided observation optical paths, so that the design on the optical path for camera observation is compared with the second embodiment. Increases freedom.

  For example, in the second embodiment, when an image twice as large as visual observation is to be obtained with a camera, an imaging lens having a focal length of 360 mm to 400 mm is required in the optical path for camera observation. Since the diameter is large and the focal length is long, the foot space on the optical path for camera observation becomes large, and there are restrictions on the arrangement of other units.

  Therefore, by arranging one imaging lens with a long focal length before the division, the plurality of imaging lenses after the division have a short focal length, a small diameter, and a small foot space on the optical path for camera observation. Therefore, the degree of freedom of arrangement of other units and combinations of imaging lenses having different focal lengths is increased.

  The embodiments of the present invention have been described above with reference to the drawings. However, the present invention is not limited to these embodiments, and various modifications and changes can be made without departing from the scope of the present invention. Also good.

FIG. 1 is a side view of an inverted microscope according to the first embodiment of the present invention. It is a front view of the inverted microscope shown by FIG. FIG. 2 is a top view of the inverted microscope shown in FIG. 1. It is a graph of the wavelength characteristic of the 1st dichroic mirror used in the modification of 1st embodiment. It is a graph of the wavelength characteristic of the 2nd dichroic mirror used in the modification of 1st embodiment. It is a graph of the wavelength characteristic of the 3rd dichroic mirror used in the modification of 1st embodiment. It is a top view of the inverted microscope by 2nd embodiment of this invention. It is a side view of the inverted microscope of a prior art example. It is a front view of the inverted microscope shown by FIG. It is a front view of the inverted microscope shown by FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Inverted microscope main body, 2 ... Stage, 3 ... Sample, 4 ... Objective lens, 5 ... Revolver, 6 ... Revolver holding stand, 7 ... Rotation focusing knob, 8 ... Lamp house, 9 ... Light source, 10 ... Projection tube 11 ... mirror cassette, 12 ... mirror, 13 ... optical path switching prism, 13a ... prism, 13b ... prism, 14 ... imaging lens, 15 ... imaging lens for visual observation, 16 ... imaging lens for camera observation, 16a ... Imaging lens for camera observation, 16b ... Imaging lens for camera observation, 16c ... Imaging lens for camera observation, 16d ... Imaging lens for camera observation, 17 ... Camera port, 18 ... Half mirror for dividing optical path, 18a ... Optical path Division half mirror, 18b ... Optical path division half mirror, 18c ... Optical path division half mirror, 19 ... TV camera, 19a ... Imaging surface, 20 ... TV camera, 20a ... Photography 21: TV camera, 21a: imaging surface, 22 ... TV camera, 22a ... imaging surface, 23 ... mirror, 24 ... eyepiece, 27 ... camera adapter, 28 ... camera, 28a ... imaging surface, 36 ... surface plate, 37a ... Bracket, 37b ... Bracket, 37c ... Bracket, 38a ... Bracket, 38b ... Bracket, 38c ... Bracket, 38d ... Bracket

Claims (10)

An objective lens that creates a parallel light flux from the light from the object;
An optical path dividing unit for dividing an observation optical path extending through the objective lens into an optical path for camera observation and an optical path for visual observation;
An imaging lens for visual observation arranged on the optical path for visual observation;
An imaging lens for camera observation arranged on the optical path for camera observation;
A microscope provided with an eyepiece that enables visual observation of an image formed by a visual observation imaging lens.   The microscope according to claim 1, wherein the focal lengths of the imaging lens for camera observation and the imaging lens for visual observation have different focal lengths.   3. The microscope according to claim 2, further comprising camera observation optical path dividing means for dividing the camera observation optical path into a plurality of parts.   4. The microscope according to claim 3, wherein the camera observation imaging lens is disposed in the optical path for camera observation before being divided.   4. The microscope according to claim 3, wherein the camera observation imaging lens is disposed in the divided optical path for camera observation.   6. The microscope according to claim 5, further comprising a plurality of camera observation imaging lenses, wherein each of the plurality of camera observation imaging lenses is arranged in a plurality of divided camera observation optical paths.   4. The microscope according to claim 3, wherein the optical path dividing means for camera observation includes an optical element that performs both optical path division and wavelength separation.   4. The microscope according to claim 3, wherein at least one camera observation imaging lens is disposed separately in the optical path for camera observation before and after being divided.   The camera observation imaging lens according to claim 3, comprising a plurality of camera observation imaging lenses (including the above-mentioned camera observation imaging lenses), each of the plurality of camera observation imaging lenses having an optical path for camera observation before being divided, and A microscope arranged in a plurality of divided optical paths for camera observation.   10. The microscope according to claim 1, wherein at least one of the imaging lenses for camera observation is configured to be detachable with respect to each optical path.

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