JP2006249045A - Cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic containing a raw material derived from an animal, having high effectiveness on skin-beautifying action, particularly by dissolution of chapped skin and having little anxiety to safety. <P>SOLUTION: The cosmetic comprises an extract of chicken fertilized egg. In the cosmetic, the chicken fertilized egg is 8-12 age in day. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

The present invention relates to a cosmetic.

The skin has a function to protect the living body from various environmental changes and stimuli from the outside world, and the stratum corneum located in the outermost layer of the skin epidermis plays a buffering effect as a boundary between the outside world and the living body. . The stratum corneum needs moderate moisture to respond flexibly to various activities of the living body and to maintain elasticity, flexibility, protective function, etc. It is known that the loss of skin elasticity, flexibility, protective function, etc. will cause various troubles.

In addition, when the skin is exposed to ultraviolet rays, the skin is affected variously. At that time, active oxygen, lipid peroxide, and the like generated in the skin cause inflammation and cause great damage to the skin tissue. These damages cause loss of moisture, texture and texture of the skin, and further affect the dermis, forming wrinkles and the like, which causes photoaging.

For the purpose of eliminating such skin troubles, skin cosmetics such as skin creams and skin lotions are widely used. Conventionally, in cosmetics such as skin cosmetics, extracts of placenta, skin and nape, such as cows, have been used as raw materials for the purpose of preventing skin troubles as described above, and for beautifying skin by eliminating roughness. Since the occurrence of BSE, the so-called mad cow disease, anxiety about the safety of animal-derived ingredients has increased.

For this reason, there is an increasing tendency to obtain physiologically active substances having functions as described above from plants and fish, and various raw materials have been studied. However, when using raw materials derived from plants and fish, the effectiveness is high. Sometimes it was not enough. For this reason, development of the raw material of the animal-derived cosmetics with the high effectiveness of the skin beautifying effect mentioned above and a little anxiety about safety was desired.

Patent Document 1 discloses a physiological health effect and skin conditioning effect on skin and hair by using a cosmetic composition containing a water-soluble chicken egg extract extracted from a chicken egg or a hydrolyzate thereof and a polyphenol derivative. It is disclosed that it can be obtained. However, chicken eggs have not been studied in detail, and the use of fertilized eggs as chicken eggs is not disclosed.

JP 2002-205913 A

In view of the above-mentioned present situation, the present invention aims to provide a cosmetic containing an animal-derived raw material that has a high effectiveness of beautifying skin due to the elimination of roughness and is less worried about safety.

The present invention is a cosmetic comprising an extract of a chicken fertilized egg.
The fertilized chicken eggs are preferably 8-12 days old.
The chicken fertilized egg extract is preferably an enzyme-decomposed extract obtained by enzymatic treatment of a chicken fertilized egg with a protease.
The cosmetic is preferably a skin cosmetic.
Hereinafter, the present invention will be described in detail.

The cosmetic of the present invention contains an extract of a chicken fertilized egg. The use of such fertilized egg extract as a raw material for cosmetics has not been sufficiently studied, but in the present invention, the physiologically healthy effect in the skin by the action of the chicken fertilized egg extract. And it discovered that the skin conditioning effect could be expressed. As a result, the effect of beautifying the skin by eliminating the roughening property can be obtained in the skin.

The above-mentioned fertilized eggs are traditionally eaten as tonic foods in the Southeast Asian countries including the Philippines, and in Western countries such as Sweden, the fertilized egg processed powder is called “Libido”. It is marketed as a tonic food. Thus, the fertilized chicken eggs are stably mass-produced, and there is no problem in supply. Furthermore, the production site is managed hygienically and is systematically stable. Moreover, since a chicken fertilized egg is used for manufacturing vaccines such as influenza, there is little anxiety about safety.

The fertilized chicken eggs are preferably 8-12 days old. When using an extract obtained using an 8-12 day-old chicken fertilized egg, the moisturizing effect, recovery of elasticity, etc. can be more effectively expressed in the skin. If it is less than 8 days old, the component may be insufficiently developed, and if it is more than 12 days old, blades, skeletons and the like may be formed, which may hinder the extraction process. In general, the above-mentioned chicken fertilized eggs are born by incubation for 21 days.

Examples of chickens that can be used in the present invention include Chordata, Birds, Pheasidae, Phasianidae, Chicken Gallus gallus (Domestic), Red-tailed quail, Red-tailed tiger shark, White-headed hawk Long-tailed duck, red-billed chick, red-billed sardine, red-bellied madder, red-bellied toad, red-breasted charcoal, red-bellied red-bellied bird, arakana, lobster, quail-bird, oodan, silky quail, quail, Utai chan, australop, red-bellied rock Long-tailed duck, butterflyfish, baboon, brown coconut, wig crabs, kawachiyako, goshkikawachiyako, kinza surao, kinzasa Tokko, Kimpa, Gyfujidori, Guinsuzunamichabo, Kumamotoshu, Kuremoto, Kuroshikiwa, Gempeichabo, Cochin, Koeyoshi, Kokushoku Kokkei, Crow Kokkei, Kokushokuekinankinshamo, Kokushokuoshamo, Kokushokushashamo, Kokushokushashama Pepper, Pepper, Pepper, Coddle, Golden Sebright Bantam, Papilio surao, Pepper, Plum, Pepper, Papilio, Plover, Pepper, Drosophila, Pepper Uminohiki, White-faced Giant Plover, White-faced Giant, White-faced Shrimp, Silver Sebright Bantam, White-faced White Shark, White-faced White Shark Sasatsumadori, Shirozasato Tokiko, Sorosa Saminohiki, Shiroza Saminohi Cabobo, Saddlebird, Nagoya, Nankinshamo, Hakkosh Tokko, Kakushokukokei, Shirokokkei, Hakushokuzurao, Hakushokuohashaku, Hakushoku Hakushokuhakaku Bantam, hakshokushamo, hakushokubo, hakakukutomaru, hakokuminohiki, hakokukuregu horn, bantam, bearded dragonfly, swanfish, cinnabar, brammer, plymouth rock, pekin bantam, porish, minohiki, minohikichabo, miyagi gankei Any kind of egg such as Rhode Island Red, and any of these crossbred eggs can be used.

The method for extracting the chicken fertilized egg is not particularly limited. For example, the chicken fertilized egg is directly or hydrolyzed using an acid, an alkali or an enzyme, and then an extraction solvent of water or a water-soluble solvent is used. Can be extracted using. Moreover, it can also hydrolyze after extracting using an extraction solvent. Especially, since allergenicity falls, it is preferable to extract using an extraction solvent after decomposing | disassembling using an enzyme.

Examples of the extraction solvent include water, physiological saline, alcohols (for example, lower alcohols such as methanol, absolute ethanol and ethanol, polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and ethyl. Examples include ethers such as ether, dioxane, acetonitrile, and ethyl acetate, xylene, benzene, and chloroform. Especially, it is preferable to use water from the point which can obtain the extract with high effectiveness of the skin beautification effect by elimination of roughness. These may be used alone or in combination of two or more.

The extract may be prepared by arbitrarily drying, concentrating or diluting depending on the dosage form / form of the cosmetic to be blended.
The method for producing the extract is not particularly limited, but it may be usually in the range of the boiling point of the solvent at normal temperature and normal pressure. After extraction, it is adsorbed, decolored and purified using filtration or an ion exchange resin. The solution, paste, gel, and powder may be used. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decoloration may be added as long as the effect is not affected. An activated carbon column or the like may be used, and usual means generally applied depending on the extracted substance may be arbitrarily selected.

More preferably, the chicken fertilized egg extract is an enzyme-decomposed extract obtained by enzymatic treatment of a chicken fertilized egg with a proteolytic enzyme. Rather than using an extract obtained by directly extracting chicken fertilized eggs with water, physiological saline, lower alcohol or the like, it is possible to reduce the allergenicity by using an extract obtained by reducing the molecular weight by enzymatic degradation. Moreover, when the skin cosmetics containing the extract of the said enzyme degradation product are used, the improvement effect with respect to roughness can be obtained effectively. Furthermore, when the chicken fertilized egg extract is an enzyme-decomposed extract obtained by enzymatic treatment with a proteolytic enzyme, it is more preferable to use water as an extraction solvent. Thereby, the extract with high effectiveness about the improvement effect with respect to roughness can be obtained.

Examples of the protease include thiol proteases such as papain, cathepsin B1, ficin, bromelain, chymopapain, Clostridium histolyticum proteinase, and Streptococcal proteinase; trypsin, plasmin, E. coli and the like. Examples include serine proteases such as E. coli protease II, elastase and proteinase K; acidic proteases such as pepsin, and other endopeptidases.

Since the suitable pH of the proteolytic enzyme varies depending on the type, it is preferable to perform the enzyme treatment by adjusting the pH appropriately depending on the proteolytic enzyme used, but the pH is usually adjusted to 1.8 to 8.0. It is preferable to adjust and perform the enzyme treatment.

Although the addition amount of the said proteolytic enzyme changes with kinds of enzyme to be used, it is preferable that it is 0.01-2.0 mass parts with respect to 100 mass parts of said fertilized eggs. In addition, the enzyme treatment is efficient when the raw material is placed in a tank with a temperature controller and stirred with a stirrer or the like.

In the enzyme treatment using the above-mentioned proteolytic enzyme, the reaction temperature may be room temperature or high temperature, but is preferably maintained in the range of 50 to 80 ° C. The enzyme treatment time may be appropriately determined depending on the type and amount of the proteolytic enzyme to be used, but the enzyme treatment is preferably performed for 2 to 48 hours. Subsequently, after heating to 85-100 degreeC normally, it cools to 10-40 degreeC and hold | maintains for a fixed time.

The insoluble matter generated by the enzyme treatment can be removed by filtration, centrifugation, decantation, or the like to obtain an extract of the target enzyme degradation product.
The extract of the above enzymatic degradation product is adjusted for pH as necessary, and the activity is not lost even if the polymer is removed by gel filtration or ultrafiltration. Furthermore, anion and cation exchange resins Fractionation may be performed by either column method or batch method. In addition, the enzyme degradation product or a fractional component thereof may be concentrated by a method such as vacuum concentration, ultrafiltration or freeze concentration, or may be dry powdered by a method such as freeze drying, spray drying or flat plate drying. it can.

In the cosmetic of the present invention, the extract of a fertilized chicken egg is preferably 0.0001 to 20% by mass in 100% by mass of the cosmetic. If it is less than 0.0001% by mass, the effect of beautifying the skin due to the elimination of the roughening property may not be obtained. Even if it exceeds 20 mass%, there is a possibility that the increase of the effect commensurate with the increased amount is not observed. More preferably, it is 0.01-5 mass%, and it is still more preferable that it is 0.1-2 mass%.

In addition to the components described above, the cosmetic of the present invention can be appropriately selected from the following substances depending on the required efficacy and effects. For example, commonly used excipients, fragrances, fats and oils, surfactants, moisturizers, whitening agents, pH adjusters, binders, polyhydric alcohols, essential oils and fragrances, thickening And various cosmetic ingredients such as agents, preservatives, antioxidants, ultraviolet absorbers, pigments, plant pulverized products and herbal medicines, inorganic salts and acids, cleaning agents, and emulsifiers.

As the fats and oils, generally used in cosmetics, for example, soybean oil, almond oil, paraffin, cetanol, avocado oil, olive oil, jojoba oil, coconut oil, palm oil, nuka oil, jojoba oil, egg yolk oil, Vegetable oils such as castor oil, squalane, avocado oil, lanolin, liquid paraffin, white petrolatum; animal fats such as beef tallow, pork fat, horse fat, turtle oil, mink oil, perserine oil, squalane; methylpolysiloxane, behenyl alcohol, Synthetic fats and oils such as glyceryl tricaprate, glyceryl trioctanoate, liquid paraffin, DHA, EPA and the like can be mentioned.

Examples of the surfactant include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate, and diethanolamide laurate; cations such as stearyltrimethylammonium chloride, cetyltrimethylammonium chloride, and benzalkonium chloride. Non-ionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, polymer Surfactants, fluorosurfactants, silicone surfactants, polypeptide derivatives, natural surfactants and the like can be mentioned.

Examples of the humectant include synthetic humectants such as glycerin, propylene glycol, 1,3-butylene glycol, polyoxyglycerin (26E.O), pyrrolidone carboxylate soda and pantethein-S sulfonate; hyaluronic acid and elastin. , Keratin, dermatan sulfate, collagen, placenta extract, royal jelly, microbial fermentation liquids such as chitin, chitosan, pectin, and other plant and animal extracts, natural oils such as vegetable oils and their esterified products, derivatives, etc. Is mentioned.

Examples of the whitening agent include ascorbic acid and salts and derivatives thereof, arbutin and derivatives thereof, placental extract, and extracts derived from other plants and animals.

Examples of the pH adjuster include organic acids such as citric acid, lactic acid, salicylic acid, tartaric acid, malic acid, benzoic acid, sodium citrate, fumaric acid, sodium monohydrogen phosphate, potassium dihydrogen phosphate, inorganic acids and the like. Examples include salts.

As the binder, generally used in cosmetics, for example, sodium carboxymethylcellulose, methylcellulose, sodium salt, casein, pectin, starch, sodium alginate, polyvinyl alcohol, polyvinylpyrrolidone, locust bean gum, carrageenan, Examples include agar and carbopol.

Examples of the polyhydric alcohols include glycerin, propylene glycol, sorbitol, polyethylene glycol, 1,3-butylene glycol, dipropylene glycol, polyoxyethylene glycerin (26E.C) and the like.

The essential oils and fragrances include lavender oil, jasmine oil, rose oil, lemon oil, orange oil, mint oil, thyme oil, ginger oil, fennel oil, cedar oil, hiba oil, raspberry oil, cypress oil, rose oil, Examples include natural and synthetic fragrances such as eucalyptus oil, camphor, peppermint oil, spearmint oil, geraniol, mandarin oil, spruce, citronellol, terpenes, coffee oil, tea oil, coconut oil, and raspberry ketone.

Examples of the thickener include carboxymethyl cellulose, carboxymethyl cellulose Na, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer, polyvinyl alcohol, sodium alginate, carrageenan, xanthan gum, bentonite and the like.

Examples of the preservative include paraoxybenzoic acid esters such as methyl paraben, ethyl paraben, propyl paraben, and butyl paraben, phenoxyethanol, ethanol, and dehydroacetic acid.

Examples of the antioxidant include alcorbic acid, EDTA4Na, EDTA2Na, butyloxytoluene, hydroxybutyltoluene, butylhydroxyanisole (BHA), dibutylhydroxytoluene (BHT), and the like.

The ultraviolet absorber is not particularly limited as long as it has a property of selectively absorbing ultraviolet rays. For example, oxybenzone, oxybenzone sulfonic acid, tetrahydroxybenzophenone, sodium dihydroxydimethoxybenzophenone sulfonate, Examples include dihydroxydimethoxybenzophenone, dihydroxybenzophenone, sinoxate, methyl diisopropylcinnamate, octyl methoxycinnamate, glyceryl paraaminobenzoate, octyl paraaminobenzoate, and the like.

Examples of the pigment include bengara, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, zinc oxide, sericite, mica, talc, carbon, medicinal charcoal and the like.

As the above plant pulverized products and herbal medicines, lemon peel, seaweed, cypress, hiba, fukkas, rice bran, shrub, ginger, licorice, chimpi, spruce, yuzu, tokyo, carrot, mint, keihi, Japanese cypress, mugwort, Dokudami, Momonoha, Chamomile, Aloe, Jasmine, Rosehip, Lavender, Guava, Ogon, Cuco, Reishi, Elderberry, Ashitaba, Uguigi, Burdock, Black Sesame, Black Rice, Fuji Sanchi Ginseng, Ginseng, Ginseng, Ginseng , Pulverized products such as mugwort and nematode and extracts thereof.

Examples of the inorganic salts and inorganic acids include sodium chloride, sodium hydrogen carbonate, sodium carbonate, boric acid, borax, sodium sulfate, sodium sulfide, potassium sulfide, sodium nitrate, calcium nitrate, ammonium sulfate, sodium thiosulfate, calcium hydrogen phosphate Potassium chloride, ammonium chloride, sodium phosphate, sodium hyposulfite, calcium thiosulfate, sulfur, sodium sesquicarbonate, magnesium sulfate, magnesium chloride, anhydrous silicic acid, metasilicic acid, boric acid and the like.

Examples of the cleaning agent include fatty acid soaps, anionic surfactants such as sodium lauryl sulfate and alkyl ether carboxylates, and aliphatics.

As the emulsifier, a substance known as a cosmetic raw material can be used. Particularly preferred are sucrose fatty acid esters such as sucrose laurate, sucrose myristic ester, sucrose palmitate, sucrose stearate, sucrose oleate and sucrose erucate; tetraglycerin mono Stearic acid ester, tetraglycerin monooleic acid ester, tetraglycerin tristearic acid ester, hexaglycerin monolauric acid ester, hexaglycerin monomyristic acid ester, hexaglycerin monostearic acid ester, hexaglycerin tristearic acid ester, decaglycerin monolauric acid ester , Decaglycerin monomyristic acid ester, decaglycerin monostearic acid ester, decaglycerin distearic acid ester, decaglycerin Tristearic acid ester, decaglycerin pentastearic acid ester, decaglycerin monoisostearic acid ester, decaglycerin diisostearic acid ester, decaglycerin pentaisostearic acid ester, decaglycerin monooleic acid ester, decaglycerin trioleic acid ester, decaglycerin pentaolein Polyglycerin fatty acid esters such as acid esters; sorbitan branched fatty acid esters such as sorbitan monoisostearate, sorbitan sesquiisostearate, sorbitan triisostearate; soy lecithin, egg yolk lecithin, hydrogenated soy lecithin, hydrogenated egg yolk lecithin, and these Lysolecithin and / or hydrogenated lysolecithin, hydroxyl, which was made into a monoacyl form by enzymatic treatment Lecithins such as the hydroxy lecithin; monoisostearate, glyceryl sesquiisostearate, glyceryl glycerol branched fatty acid esters such diisostearate, glyceryl: can be exemplified, and can be used in combination of one or more.

Furthermore, in addition to the above-described cosmetics, the cosmetics of the present invention include milk-derived components such as lactose, milk and condensed milk as necessary, other inorganic pigments such as titanium and talc, isopropylmethylphenol and chlorohydrochloride. Bactericides such as rohexidine and chlorohexidine gluconate; vitamins such as vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin P, CoQ10, CoQ9, CoQ8, thioctic acid, coenzymes, anhydrous silicic acid, synthetic aluminum silicate, etc. Fluidizers for pharmaceuticals; pharmaceuticals, quasi drugs, and tar-type pigments for cosmetics can be appropriately blended.

In addition to the components described above, various cosmetic components such as medicinal components for the purpose of beauty, facial treatment, skin treatment, and the like can be appropriately blended in the cosmetic of the present invention.
The above-mentioned medicinal ingredients include: Ashitaba extract, Avocado extract, Achacha extract, Altea extract, Arnica extract, Aloe extract, Apricot extract, Apricot extract, Ginkgo biloba extract, Fennel extract, Turmeric extract, Oolong tea extract, Echinashi leaf extract, Ogon extract, Oat extract, barley extract, Dutch mustard extract, orange extract, seaweed extract, seawater dried product, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, kawara mugi extract, licorice extract, calkade extract, kiwi extract , Kina extract, Cucumber extract, Guanosine, Kumazasa extract, Walnut extract, Grapefruit extract, Clematis extract, Yeast extract, Burdock extract, Comfrey extract, Cowberry extract Psycho extract, Saitai extract, Salvia extract, Soybean extract, Sasa extract, Hawthorn extract, Shiitake extract, Giant extract, Siphon extract, Linden extract, Shimotake extract, Pepper extract, Ginger root extract, Birch extract, Japanese cedar extract, Honeysuckle extract, Prunus Kizuna extract, hawthorn extract, elderberry extract, yarrow extract, mint extract, mallow extract, seaweed extract, thyme extract, thyme extract, clove extract, chigaya extract, chimpi extract, spruce extract, dokudami extract, tomato extract, natto extract, Carrot extract, Novara extract, Hibiscus extract, Bakumondou extract, Parsley extract, Honey, Parietalia extract, Hikiokoshie , Bisabolol, dandelion extract, burdock extract, butterfly extract, butcher bloom extract, grape extract, propolis, loofah extract, peppermint extract, body sage extract, hop extract, pine extract, maronier extract, mizubasho extract, mukuroji extract, peach leaf extract, cornflower Extract, Eucalyptus extract, Yuzu extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Lotus root extract, Rose extract, Rosemary extract, Roman chamomile extract, Royal jelly extract and the like.

Further, anti-inflammatory agents such as ε-aminocaproic acid, glycyrrhizic acid, dipotassium glycyrrhizinate, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, hydrocortisone, vitamins A, B2, B6, C, D, E, calcium pantothenate, biotin , Vitamins such as nicotinamide, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as carotenoids, flavonoids, tannins, lignans, saponins, γ-oryzanol, vitamin E derivatives Blood circulation promoter such as β-carotene, proteolytic enzyme wound healing agent, cephalanthin, chili pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocolic acetate Errol, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinylesteradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, camphor, salicylic acid, nonyl acid Vanillylamide, nonanoic acid vanillylamide, piroctone olamine, glyceryl pentadecanoate, 1-menthol, pyrrolidone carboxylate of menthol, mononitroguaiacol, urea, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, Cantharis tincture, cyclosporine, hydrocortisone, analgesics, tranquilizers, antihypertensives, antibiotics, antihistamines, antibacterial A sex substance etc. can also be arbitrarily blended.

The cosmetics of the present invention include, for example, topical or systemic skin cleansers or skin cosmetics; medicinal and cosmetic preparations applied to the scalp and hair; bath preparations used in bath water; Odor / deodorant: skin patch sheet, cosmetic sheet, cosmetic cotton, sanitary goods, sanitary cotton, wet tissue and the like. The cosmetic of the present invention includes quasi-drugs, designated quasi-drugs, external medicines, and the like, and the dosage form can be arbitrarily selected according to the purpose.

Specifically, lotions, emulsions, creams, ointments, gels, lotions, oils, packs, mists, facial cosmetic sheets and other skin cosmetics, shaving agents, facial cleansers, skin cleansers, shampoos, rinses , Hair treatments, hair preparations, perms, hair arts, hair dyes, hair cosmetics such as hair growth and nourishing hair, foundations, lipsticks, blushers, eye shadows, eyeliner, mascara makeup cosmetics, perfumes, skin Deodorant, antiperspirant, bathing agent, body rinse and the like.

Especially, it is preferable that the cosmetics of this invention are skin cosmetics. When used as a skin cosmetic, effects such as a moisturizing effect and recovery of elasticity can be sufficiently obtained.
The cosmetic of the present invention can be produced according to a conventional method.

The cosmetic of the present invention contains an extract of a chicken fertilized egg. For this reason, especially when used as a skin cosmetic, effects such as a moisturizing effect and elasticity recovery can be exhibited. Moreover, there is little anxiety about safety.

Since the cosmetic of the present invention has the above-described configuration, effects such as a moisturizing effect and elasticity recovery can be exhibited. Therefore, it can be suitably used particularly as a skin cosmetic.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. In the examples, “parts” and “%” mean “parts by mass” and “% by mass” unless otherwise specified.

Production Example 1
A frozen 9-day-old chicken fertilized egg was thawed, the shell was removed, and the contents were homogenized. 2500 mL of pure water was added to the contents (2.4 Kg) of 50 homogenized fertilized eggs, heated to 80 ° C., and held for 15 minutes. Thereafter, the mixture was put in a 10 L stainless steel beaker and cooled to 60 ° C., and 50 g of proteolytic enzyme (pancreatin, derived from pig) was added. The enzyme reaction was carried out by homogenizing with a mixer for 5 hours under conditions of pH 7.0 and 60 ° C. At the end of the reaction, the pH was adjusted to 6 with lactic acid, further heated to 90 ° C. and held for 30 minutes. Thereafter, the mixture was cooled to room temperature and allowed to stand for 10 hours, and the supernatant was collected by decantation. The obtained supernatant is stored frozen, thawed, filtered using a filter paper, then filtered using a membrane filter, and filled into a sterile bottle to obtain about 4.3 L of a fertilized egg extract. It was.

Examples 1 to 3 and Comparative Example 1
The amount of transdermal moisture transpiration and stratum corneum moisture when the extract obtained in Production Example 1 was applied to the skin were examined by the following test method.

1. One group of five hairless mice of 9 weeks of age (male) at the start of the experimental animal test used in this example and comparative examples was used.

2. 2. Measurement of transdermal moisture transpiration 2-1. Measuring Device and Condition Transcutaneous moisture transpiration (hereinafter abbreviated as TEWL) was measured as follows using a continuous sweat measuring device Hydrograph AMU-100 (manufactured by K & S Inc.). A 1 cm 2 capsule was brought into close contact with the skin, nitrogen gas was introduced into the capsule (300 ml / min), and the amount of water vapor in the nitrogen gas was measured before being sent to the capsule and after being recovered from the capsule. From this difference in value, the amount of water (milligram) transpired from 1 square centimeter of skin per minute was calculated and used as TEWL.

2-2. Sample and Experimental Method A sample was prepared by mixing 50% ethanol aqueous solution (base) with the chicken fertilized egg extract obtained in Production Example 1 as described in Table 1. First, 0.05 ml of this sample was applied to the back skin (diameter of about 2.5 cm) of a hairless mouse in which TEWL was measured in advance once a day at a frequency of 5 times a week (preliminary application). . Thereafter, ultraviolet B wavelength (UVB) was irradiated once at 0.15 J / cm ^{2 on} the third day from the final application of the pre-application. Then, the TEWL after 3 days and 4 days after the irradiation is measured, and the TEWL fluctuation rate (= 3 days after the irradiation or the relative value indicating how much the TEWL has fluctuated due to UVB with reference to the TEWL at the start of the test. The TEWL value after 4 days / the TEWL value at the start of the test) was calculated, and the average values of the base group and each group were compared. The results are shown in FIG.

2-3. The effect determination method TEWL varies depending on the degree of skin permeation barrier function disruption, and the TEWL variation rate indicates that the greater the value, the greater the degree of skin permeation barrier function disruption. In addition, when only the base is applied, the skin permeation barrier function collapses due to UVB as in the case of no application. Therefore, the case where the TEWL fluctuation rate was low compared with the base coating group in a state of natural skin permeation barrier function collapse was determined to be effective.

3. Measurement of stratum corneum moisture content In the above-mentioned measurement of transdermal moisture transpiration, the amount of change in stratum corneum moisture content at the start of the test and before UVB irradiation is measured using IMPEDANCE METER SKICON-200 (manufactured by IBS Corporation). did. The results are shown in FIG.

From FIG. 1, a significant rough skin inhibitory effect was recognized in a concentration-dependent manner by adding 0.1% or more of the fertilized egg extract. From FIG. 2, the tendency which increases a stratum corneum moisture content by adding a fertilized egg extract was recognized.

Examples 4-9
Skin cosmetics (skin lotion, milky lotion, cream, sheet pack, powder pack, bathing agent) containing an extract of the chicken fertilized egg obtained in Production Example 1 were prepared with the following composition.

Example 4 (skin lotion)
[composition]
Raw material component amount (% by mass)
Ethanol 10.0
Polyoxyethylene hydrogenated castor oil (60 EO) 1.0
Glycerin 3.0
1,3-butylene glycol 2.0
Dipropylene glycol 3.0
Polyethylene glycol 1500 1.0
Phosphate 0.5
Methylparaben 0.1
Chicken fertilized egg extract (9 days) 0.1
Purified water remaining

Example 5 (milky lotion)
[composition]
Raw material component amount (% by mass)
(A component)
Stearic acid 1.0
Stearic acid glyceryl ester 2.0
Cetanol 1.0
Cholesterol 0.5
Vaseline 2.0
Squalene 5.0
Liquid paraffin 5.0
Silicone oil 1.0
Ptylparaben 0.1
Chicken fertilized egg extract (9 days) 0.5
(B component)
Acyl glutamate 1.0
Alkyl-modified carboxyvinyl polymer 0.2
Glycerin 2.0
Dipropylene glycol 3.0
Remaining amount of purified water totaling 100 [Preparation method]
The emulsion was prepared by adding the component B shown above to the component A and stirring.

Example 6 (cream)
[composition]
Raw material component amount (% by mass)
(A component)
Stearic acid 2.0
Stearic acid glycerin ester 2.0
Cetanol 3.0
Cholesterol 0.5
Vaseline 2.0
Squalene 5.0
Liquid paraffin 10.0
Silicone oil 1.0
Butylparaben 0.1
Chicken fertilized egg extract (9 days) 1.0
(B component)
Acyl glutamate 1.0
Carboxyvinyl polymer 0.15
Alkyl-modified carboxyvinyl polymer 0.15
Glycerin 5.0
Dipropylene glycol 3.0
Purified water remaining amount (preparation method)
Each of the A and B components shown above was dissolved by heating at 80 ° C. and then cooled to 30 ° C. while mixing and stirring to prepare each skin cream.

Example 7 (sheet-shaped pack)
[composition]
Raw material component amount (% by mass)
Chicken fertilized egg extract (9 days) 1.0
Sodium polyacrylate 2.0
Polyvinylpyrrolidone 0.5
Glycerin 25.0
1,3-butylene glycol 10.0
Paraben 0.1
Fragrance 0.1
L-Menthol 0.1
Impregnated nonwoven fabric 10.0
Purified water remaining

Example 8 (powdered pack)
[composition]
Raw material component amount (% by mass)
Chicken fertilized egg extract (9th) 2.0
Sodium alginate 15.0
Talc 20.0
Citric acid 0.2
Sodium citrate 0.6
Paraben 0.1
Perfume Micro-grilled gypsum

Example 9 (bathing agent)
[composition]
Raw material component amount (% by mass)
Chicken fertilized egg extract (9 days) 0.5
Giant extract 0.1
Carrot extract 0.05
Chinpi powder 1.0
Macadamia nut oil 0.05
Hiba oil 0.1
1,3-butylene glycol 1.0
Xylitol 0.5
Polyoxyethylene sorbitan monolaurate 1.5
Anhydrous sodium sulfate

In Examples 4 to 9, lotions, emulsions, creams, sheet packs, powder packs, and bath preparations containing the chicken fertilized egg extract obtained in Production Example 1 could be suitably prepared.

The cosmetic of the present invention can be suitably used as a skin cosmetic or the like.

It is the schematic which showed the TEWL fluctuation rate of the 3rd day and the 4th day after UVB irradiation. It is the schematic which showed the variation | change_quantity of the stratum corneum moisture content.

Claims (4)

A cosmetic comprising an extract of a fertilized chicken egg. The cosmetic according to claim 1, wherein the chicken fertilized egg is 8 to 12 days old. 3. The cosmetic according to claim 1 or 2, wherein the chicken fertilized egg extract is an enzyme-decomposed extract obtained by subjecting a chicken fertilized egg to a proteolytic enzyme. The cosmetic according to claim 1, 2 or 3, wherein the cosmetic is a skin cosmetic.

Images