JP2006177692A - Mold forming die for forming sample embedded body for microscopic observation, sample embedded body for microscopic observation, and its fixing method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To fix a cut surface of a sample with satisfactory repeatability and to fix it with an angle of the cut surface held with satisfactory repeatability when mounting the sample on a sample holder, such as a sample holder for a microtome or a sample holder for an ultramicrotome, in order to observe the cut surface (observing object surface) of the sample which is an object under inspection in observation or inspection analysis by means of an optical microscope, an electronic microscope, etc. <P>SOLUTION: A molding die recessed part A for therein putting/molding resin for sample embedding, comprises: a cube-shaped recessed-part area 1 or rectangular solid-shaped recessed-part area 1 equipped with a mold wall part 6 for a cutting object surface molding or observing object surface molding on one end surface or two opposite end surfaces; and projection-shaped recessed-part areas 2 and 2 for molding a lockingly fixing part which projects relative to the other two opposite end surfaces in the relevant recessed area and which is locked on and fixed to an observed sample holder of a microscope. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention is to produce a sample embedment for microscopic observation by embedding (holding) a sample, which is an object to be inspected in an observation or inspection analysis using an optical microscope or an electron microscope, with a transparent resin. The present invention relates to a mold for molding a sample embedding body, a sample embedding body, and a fixing method thereof.

  In making an observation sample (sample) for an optical microscope or an electron microscope, the sample is embedded and fixed and held in a transparent resin in order to prevent distortion due to the impact of the cutting or to facilitate handling. There is a way.

  The sample embedding body (sample block) embedded with transparent resin must be trimmed according to the size of the sample shape, but in order to reduce the labor, the sample embedding body must be trimmed. 2. Description of the Related Art Molding molds have been used that can form the vicinity of the tip of a body thinly.

  Conventionally, there are various shapes of the sample embedding body (sample block). Among them, for example, the shape of the sample embedding body described in Non-Patent Document 1 is shown in FIG. A single-ended type having a shape with inclined surfaces 22 and 22 on both sides of one end surface 21 which is an observation target surface (or a cutting target surface) of the rectangular parallelepiped (hexahedron-shaped) embedded body 20 as shown in FIG. Inclined surfaces 22 and 22 are provided on both sides of both end surfaces 21 and 21 facing each other in parallel, which are observation target surfaces (or cutting target surfaces) of the rectangular parallelepiped (hexahedron-shaped) embedded body 20 as shown. The double end type etc. provided with are used.

Below, the well-known nonpatent literature relevant to the technique of this invention is described.
Oken Shoji Co., Ltd., Electron Microscope Material Model Record, May 2000, p28-30 EM Japan Co., Ltd., Electron / Optical Materials Catalog No. 7, p74-77 JEOL Datum Co., Ltd., Peripheral device related to electron microscope 2002, p19

  Generally, as a method of observing and analyzing a substance with a microscope, for example, as a substance to be observed, a sample (sample) obtained by collecting a part of a package packaging material is embedded with a transparent resin, and the embedded body is cut. There is an analysis method for observing a surface (observation target surface) with a microscope.

  In order to perform more accurate analysis, images may be acquired from various observation microscopes such as an optical microscope and an electron microscope, and information may be collected and analyzed. For example, in order to observe at a higher magnification after observation with an optical microscope, the observation may be performed with a scanning electron microscope (hereinafter referred to as SEM) or a transmission electron microscope (hereinafter referred to as TEM).

  In particular, in the analysis of objects at specific locations such as minute foreign objects, the amount of sample is small, and it is difficult to make a new sample embedded body, and the same embedded body is observed and analyzed several times. Sometimes.

  In order to reduce the focus shift in observation with an optical microscope, remove the embedded body cut with the microtome from the microtome body for each sample holder without removing it from the sample holder, and observe the cut surface. Can be observed.

  However, it is difficult to make the cut surface (observation target surface) of the sample embedding body once removed from the microtome sample holder, for example, by SEM or the like, again horizontal within the depth of focus of the field of view of the optical microscope. And very inefficient. Also, in the preparation of observation slices by TEM on a microtome, the cut surface angle (observation target surface angle) of the sample embedded body reattached to the sample holder is often not parallel to the cutting knife. Since it is necessary to move the knife position and to adjust the knife angle in the vertical and horizontal directions, the work efficiency is poor.

  An object of the present invention is to observe a cut surface (surface to be observed) of a sample which is an object to be inspected in an observation or inspection analysis using an optical microscope, an electron microscope, or the like. When attaching to a sample holder, the cut surface of the sample is fixed with good reproducibility, and the cross-sectional angle is held with high reproducibility so that it can be fixed.

  In the invention according to claim 1 of the present invention, the molding die recess for filling and molding the sample embedding resin includes at least one mold wall portion for molding the surface to be cut or the surface to be observed on one end surface or opposite both end surfaces. Cuboid-shaped recessed area or rectangular parallelepiped-shaped recessed area, and a protrusion-shaped recessed area for forming an engaging and fixing portion that protrudes from the opposite opposite end faces of the recessed area and is fixed to the observation sample holder of the microscope. And a mold for forming a sample-embedded body for microscopic observation.

  In the invention according to claim 2 of the present invention, the molding die recess for filling and molding the sample embedding resin has at least one mold wall portion for molding the surface to be cut or the surface to be observed on one end surface or opposite both end surfaces. Clamp-shaped recessed area or rectangular parallelepiped-shaped recessed area, and a locking and fixing portion for protruding and locking to the observation specimen holder of the microscope, having both sides symmetrical with respect to the other opposite end faces of the recessed area It is a mold forming die for forming a sample embedding body for microscopic observation, characterized in that it is constituted by a projection-shaped recessed region for forming a sample.

  In the invention according to claim 3 of the present invention, the molding die recess for filling and molding the sample embedding resin is provided with at least one mold wall portion for molding the surface to be cut or the surface to be observed on one end surface or opposite both end surfaces. A cube-shaped recessed area or a rectangular parallelepiped-shaped recessed area and projecting with both sides symmetrical to each other at right angles to the other opposite end faces of the recessed area and for locking and fixing to the observation sample holder of the microscope It is a mold forming mold for forming a sample embedding body for microscopic observation, characterized by comprising a projection-shaped recessed area for forming a locking and fixing part.

  The invention according to a fourth aspect of the present invention is the mold molding die for molding a sample embedding body for microscopic observation according to any one of the first to third aspects, wherein the mold depth D of the concave portion of the molding die is 2 mm to 10 mm, the mold width W1 of the cube-shaped recess area or the rectangular parallelepiped recess area not including the protrusion-shaped recess area of the mold recess is 1 mm to 3 mm, and the mold width W2 including the protrusion-shaped recess area of the mold recess is 5 mm. A mold for forming a sample-embedded body for microscopic observation, characterized in that it is ˜7 mm.

According to a fifth aspect of the present invention, a sample embedding transparent resin in which a sample is mixed is filled in a molding recess of the sample embedding mold according to any one of the first to fourth aspects. A microscope-embedded sample embedding body comprising a cube-shaped recessed area or a rectangular parallelepiped recessed area and a protrusion-shaped recessed area.

  The invention according to claim 6 of the present invention is a microscope in which the sample embedding body according to claim 5 is observed at a boundary portion between the cubic-shaped recessed area or the rectangular parallelepiped-shaped recessed area of the embedded body and the protrusion-shaped recessed area. This is a method for fixing a sample embedment for microscopic observation, which is fixed to the observation sample holder.

  According to a seventh aspect of the present invention, the sample embedding body according to the fifth aspect is configured such that a boundary portion between the cubic-shaped recessed area or the rectangular parallelepiped-shaped recessed area of the embedded body and the protrusion-shaped recessed area is a microscope. This is a method for fixing a sample embedment for microscopic observation, characterized in that the sample embedded for microscopic observation is locked and fixed so as to be in contact with a screw fixing plate of a sample holder for a screw type microtome.

  According to the mold for molding a sample embedding body for microscopic observation of the present invention, the mold recess for filling and molding the sample embedding resin is at least a cubic (hexahedral) recess region or a rectangular parallelepiped (hexahedral) recess. The sample-embedded body of the present invention as a molded body molded using the mold of the present invention is composed of a cubic-shaped recessed area or a rectangular parallelepiped-shaped recessed area. Of the sample-shaped embedding body formed between the cube-shaped recess region or the rectangular parallelepiped-shaped recess region and the protrusion-shaped recess region. A locking and fixing portion for locking and fixing to the observation sample holder of the microscope can be formed at a fixed position with respect to the surface (observation target surface).

  Therefore, the sample embedding body of the present invention is reliably stable with respect to the specimen holder of the microscope at the boundary between the cubic shaped recessed area or the rectangular parallelepiped shaped recessed area and the protruding shaped recessed area of the embedded body. The surface to be cut or the observation target surface of the sample embedding body can be locked and fixed in a state of being held at a predetermined observation angle with respect to the sample holder of the microscope. Alternatively, the boundary part (or boundary surface) between the cube-shaped recessed area or the rectangular parallelepiped recessed area of the embedded body and the protrusion-shaped recessed area is in contact with the screwing plate of the sample holder for the screw type microtome of the microscope, It can be reliably and stably locked, and can be locked and fixed in a state where the cutting target surface or the observation target surface of the sample embedding body is held at a predetermined observation angle with respect to the sample holder of the microscope.

  As described above, the present invention provides a microtome for microscopic observation in order to observe a cut surface (surface to be observed) of a sample that is an object to be inspected in an observation or inspection analysis using an optical microscope, an electron microscope, or the like. When attaching to the sample holder or ultramicrotome sample holder, the cut surface (observation target surface) of the sample can be fixed stably and reliably with good reproducibility, and the angle of the cut surface (observation target surface) can be reproducibly. It can be held and fixed well.

  The mold for molding a sample embedding body for microscopic observation according to the present invention will be described in detail below along the embodiment thereof.

  FIG. 1 is an overall perspective view for explaining an example of the structure and shape of a mold, and the overall external shape is, for example, an arrow shape.

As shown in FIG. 1, a molding die recess A for filling and molding a sample embedding resin of a mold for molding a sample embedding body for microscopic observation according to the present invention includes a base F having a flat upper surface and On the upper surface of the base F, at least the bottom 5 of the mold and the mold wall surface 6 for molding the surface to be cut or the surface to be observed are provided on one end surface, and the molding surface 7 is provided on the other end surface (or The mold wall surfaces 6 and 7 for forming the surface to be cut or the surface to be observed are provided on opposite opposite end faces) on the cube-shaped recessed area or the rectangular parallelepiped recessed area 1 and on the opposite opposite end faces 4 and 4 of the recessed area 1. It is comprised by the protrusion-shaped recessed area | region 2, 2 which protrudes with respect to each.

  The cube-shaped recessed area or the rectangular parallelepiped-shaped recessed area 1, and the protrusion-shaped recessed areas 2 and 2 that protrude with respect to the opposite end faces 4 and 4 of the recessed area 1 are appropriately defined as the recessed area 1 and the recessed areas 2 and 2, respectively. It is formed to be surrounded by a surrounding member. The base F can be omitted.

  The material for forming the cube-shaped concave region 1 or the rectangular parallelepiped concave region 1, the projecting concave region 2, and the surrounding member that forms the mold concave portion A and the bottom portion 5 are particularly limited in the present invention. For example, a synthetic resin material, a metal material, a wood material, a ceramic material, a glass material, a ceramic material, or the like can be appropriately selected.

  For example, a plate-shaped surrounding member F1 formed of an appropriate material so as to surround the recessed area 1 and the recessed areas 2 and 2 may be formed by standing on the upper surface of the base F, or the recessed area A block-shaped surrounding member F2 formed of an appropriate material so as to surround 1 and the recessed areas 2 and 2 is formed on the upper surface of the base F over a predetermined area of the recessed areas 1, 2, and 2 to be the mold recessed area A. Each may be formed flush with the upper end of the mold depth direction.

  Projection-shaped recess regions 2 and 2 projecting from both side surface portions of the cube-shaped recess region or the rectangular parallelepiped recess region 1 are formed by observing the entire sample embedding body formed later by the mold recess A in a microscope observation sample holder or the like. Is a locking portion for positioning and locking at a fixed position at a fixed angle, and serves as a positioning reference portion.

  The protrusion-shaped recessed areas 2 and 2 that serve as positioning reference portions are formed in the cubic-shaped recessed areas 1 or the rectangular parallelepiped-shaped recessed areas 1 so that the protrusion-shaped recessed areas 2 and 2 can always be locked at a fixed angle with respect to an observation sample holder or the like of a microscope. There are no limitations on the shape, size, volume, etc. of the opposing end faces 4, 4 as long as they project at predetermined positions set in advance. 4 may be provided so as to project with both side surfaces 3 and 3 symmetrical with respect to the line 4, or at right angles to the opposite end surfaces 4 and 4 of the recessed area 1. It may be formed so as to protrude with both projecting side surfaces 3 and 3 that are line-symmetric (vertical).

  The mold depth D of the mold recess A, the projection-shaped recess area 2 of the mold recess 2, the mold width W1 of the cube-shaped recess area 1 or the rectangular parallelepiped recess area 1 not including the mold recess W, and the protrusion-shaped recess area 2 of the mold recess. The mold width W2 including 2 and the mold length L are not particularly limited in the present invention. For example, as an example, the mold depth D is 2 mm to 10 mm, and the protrusion of the mold recess A The mold width W1 of the cube-shaped recess area 1 or the rectangular parallelepiped recess area 1 not including the shape recess areas 2 and 2 is 1 mm to 3 mm, and the mold width W2 of the mold recess A including the protrusion-shaped recess areas 2 and 2 is The mold length L is about 3 mm to 10 mm.

The sample embedding body of the present invention is a molded body molded by a mold as shown in FIG. 1, and is for sample embedding in which a sample is mixed in a molding die recess A of a mold embedding mold for sample embedding. A molded body that is molded by filling with a transparent resin, which is a cube-shaped molding region 11 or a rectangular parallelepiped-shaped molding region 11, and projecting molding surfaces 13, each projecting from both side surfaces 14, 14 of the molding region 11, 13 is formed of protrusion-shaped forming regions 12 and 12 protruding with 13.

  For example, as shown in the plan view of FIG. 2, the sample embedding body B is a mold formed by a cube-shaped recessed area 1 or a rectangular parallelepiped-shaped recessed area 1 that does not include the protrusion-shaped recessed areas 2 and 2 of the forming mold recessed section A. Mold depth D and mold width W2 formed by a cube-shaped molding region 11 or a rectangular parallelepiped molding region 11 having a depth D, a mold width W1, a mold length L, and projection-shaped recess regions 2 and 2 of the mold recess A Projection-shaped molding regions 12, 12 serving as positioning reference portions projecting from the both side surfaces 14, 14 of the cube-shaped molding region 11 or the rectangular parallelepiped-shaped molding region 11, respectively, with projection-shaped molding surfaces 13, 13 are molded. Shaped in the shape of an arrow.

  The protrusion-shaped forming regions 12 and 12 serving as positioning reference portions are formed in the cubic-shaped forming region 11 or 11 so that the sample embedded body B can always be locked at a fixed angle with respect to the observation sample holder of the microscope. There is no limitation on the shape, size, volume, and the like as long as it is formed so as to protrude to a predetermined position with respect to the opposing opposite end faces 14, 14 of the rectangular parallelepiped shape forming region 11. It may be provided so as to have both protrusion-shaped molding surfaces 13, 13 that are line-symmetric with respect to the opposite end faces 14, 14, or to protrude in a protrusion shape, or the opposite end faces of the molding region 11. 14 and 14 may be formed so as to project in a projecting shape having both projecting molding surfaces 13 and 13 that are symmetrical with respect to each other at right angles (perpendicular).

  Further, on one end surface in the mold length L direction, there is a cutting target surface 16 or an observation target surface 16 formed by a molding wall surface 6 for forming a cutting target surface or an observation target surface of the molding die recess A. The other end face is formed by the molding surface 17 formed by the molding wall surface 7 for molding the molding recess A (or by the molding wall surface 7 for molding the surface to be cut or observation surface of the molding recess A. A cutting target surface or observation target surface 17) is formed.

  For example, as an example of the sample embedding body B, a sample 15 (polymer) cut into a wedge shape having a maximum width of 1 mm and a length of several mm in the mold recess A of the mold shown in FIG. Multi-layer film) is placed, and a one-component curable type or two-component curable transparent resin solution such as an epoxy resin is poured and cooled, solidified or reacted and cured.

  In the sample embedding body B of the present invention, for example, when the cutting target surface (observation target surface) is a single-ended sample embedding body B having only one end surface side, the length of the sample embedding body B is In the case of the double-ended sample embedding body B, a cutting target surface 16 (observation target surface) is provided on one end surface in the L direction, and a molding surface 17 is provided on the other end surface. A cutting target surface 16 (observation target surface) is provided on one end surface in the length L direction of B, and a cutting target surface 17 (observation target surface) is also provided on the other end surface.

  In the case of the single-ended sample embedded body B, the width of the cutting target surface 16 is cut in order to cut (cut) the sample embedded body B from the cutting target surface 16 (observation target surface) side of one end surface. W3 is preferably narrower than the width W1 of the molding surface 17 (W3 <W1).

  Next, a fixing method for locking and fixing the sample embedding body B for microscope observation of the present invention to the observation sample holder of the microscope will be described below. The cube-shaped molding region 11 of the sample embedding body B of the present invention or At the boundary between the rectangular parallelepiped shape forming region 11 and the protrusion-shaped recessed regions 12 and 12 protruding from both side surfaces 14 and 14 of the forming region 11, the sample embedding body B is related to the observation sample holder of the microscope. It is to be fixed.

Further, the cube-shaped molding region 11 or the rectangular parallelepiped-shaped molding region 11 of the sample embedding body B for microscopic observation of the present invention, and the projection-shaped recessed regions 12 and 12 projecting from both side surfaces 14 and 14 of the molding region 11, respectively. The boundary portion is locked and fixed so as to be in contact with the screw fixing plate of the sample holder for the screw type microtome of the microscope.

  First, the cured sample embedding body B (see FIG. 2) is removed from the molding die recess A of the mold, and as shown in the side view of FIG. 3 (a) and the top view of FIG. 3 (b), Attach to the microtome sample holder C of the microscope.

  As shown in FIGS. 3A to 3B, the microtome sample holder C of the microscope includes a pair of opposed fixed side screwing plates 31 and movable side screwing plates 32 that are separated from each other in parallel, and the movable side. The screwing plate 32 includes a screw portion 33 that moves the screwing plate 32 closer to the fixed screwing plate 31 side.

  As shown in FIG. 3 (a), a cube of the sample embedding body B (see FIG. 2) is provided between opposing surfaces of a pair of opposed fixed-side screwing plates 31 and movable-side screwing plates 32 that are separated in parallel. The shape forming region 11 or the rectangular parallelepiped shape forming region 11 is inserted, and the protruding forming surfaces 13 and 13 protruding from the protruding shape recessed regions 12 and 12 are respectively attached to one end surfaces of the two screwing plates 31 and 32. After locking the sample embedded body B so that it does not fall off, the movable side screwing plate 32 is moved toward the fixed side screwing plate 31 in the direction of the arrow, and both side surfaces of the sample embedded body B are moved. Fasten 14 and 14 in place.

  The sample embedding body B has corner corners formed by the both protrusion-shaped molding surfaces 13 and 13 of the respective protrusion-shaped recess regions 12 and 12 and both side surfaces 14 and 14 of the cube-shaped molding region 11 or the rectangular parallelepiped-shaped molding region 11. 18 (see FIG. 2) are fixed so that they are in contact with each other at corners formed by one end face of each of the screwing plates 31 and 32 and the opposite surface of the screwing plates 31 and 32. The embedded body B can be fixed at a fixed angle at a fixed position.

  After the sample embedding body B is locked and fixed to the sample holder C, the sample embedding body B is vertically cut for each sample holder C so that the cutting target surface (observation target surface) 16 faces upward. The target surface (observation target surface) 16 (cross section) becomes substantially horizontal, and observation with an optical microscope (or electron microscope) can be performed efficiently.

  In addition, when observing the sample 15 with the sample embedding body B using a transmission electron microscope (TEM), it is necessary to prepare a thin piece of the sample embedding body B attached to the sample holder C. By attaching the sample holder C to which the embedded body B is locked and fixed to the microtome, the angle setting of the cutting (cutting) knife can be simplified and the work can be performed efficiently.

  The sample holder C for microtome is made of metal, and the side surfaces of the screw fastening plates 31 and 32 are almost vertical, and the transparent resin used for embedding needs to be strong enough not to be deformed by the screwing pressure. is there. Further, when the sample embedded body B is locked and fixed to the sample holder C as shown in FIGS. 3A to 3B, the vertical and horizontal resin cross-sectional angles of the sample embedded body B are set at a constant angle. Thus, it can be fixed at a substantially constant position.

1 is an overall perspective view of a mold for forming a sample embedding body for microscopic observation according to the present invention. The top view of the sample embedding body for microscope observation of this invention. (A) is the side view of the sample holder explaining the fixing method of the sample embedding body for microscope observation of this invention, (b) is the top view. FIG. 6 is a top view of a single-end embedded plate, which is a conventional sample-embedded body for microscopic observation. It is the conventional sample embedding body for microscope observation, Comprising: The top view of a double end type embedding board.

Explanation of symbols

A ... Mold recess
B ... Sample embedding body C ... Microscope sample holder D ... Mold depth F of mold recess ... Base
F1 ... Plate-shaped surrounding member F2 ... Block-shaped surrounding member W1 ... Mold width W2 ... Mold width L ... Mold length L
DESCRIPTION OF SYMBOLS 1 ... Cuboid-shaped recessed area or rectangular parallelepiped-shaped recessed area 2 ... Projection-shaped recessed area 3 ... Projection-shaped side surface 4 ... Opposite end faces 5 of the recessed area 1 ... Bottom 6 ... Mold wall surface for forming a cutting object surface or an observation object surface 7 ... Molding surface (or mold wall surface for molding the surface to be cut or the surface to be observed)
DESCRIPTION OF SYMBOLS 11 ... Cube shape shaping | molding area | region or a rectangular parallelepiped shape shaping | molding area | region 12 ... Projection shape shaping | molding area | region 13 ... Projection shaped shaping | molding surface 14 ... Side surface 15 of a cube shape shaping | molding area | region or a rectangular parallelepiped shape shaping | molding area ... Sample 16 ... Forming surface (or cutting target surface or observation target surface)
31 ... Fixed side screwing plate 32 ... Movable side screwing plate 33 ... Screw part 33

Claims (7)

  The mold recess for filling and molding the sample embedding resin has a cube-shaped recess region or a rectangular parallelepiped recess region having at least one surface to be cut or an observation target surface on one end surface or the opposite end surfaces, and the other opposite ends of the recess region Microscopic observation sample embedding molding characterized by comprising a projection-shaped recess region for molding a locking and fixing portion for protruding and fixing to a microscope observation sample holder Molding mold.   The mold recess for filling and molding the sample embedding resin has a cube-shaped recess region or a rectangular parallelepiped recess region having at least one surface to be cut or an observation target surface on one end surface or the opposite end surfaces, and the other opposite ends of the recess region And a protrusion-shaped recess region for forming a locking and fixing portion for locking and fixing to an observation sample holder of a microscope. Mold for mold embedding of sample for microscope observation.   The mold recess for filling and molding the sample embedding resin has a cube-shaped recess region or a rectangular parallelepiped recess region having at least one surface to be cut or an observation target surface on one end surface or the opposite end surfaces, and the other opposite ends of the recess region And projecting concave and convex regions for forming locking and fixing portions for locking and fixing to the observation sample holder of the microscope. A mold for molding a sample embedded body for microscopic observation.   The mold depth D of the mold recess is 2 mm to 10 mm, the mold width W1 of the cube-shaped recess region or the rectangular parallelepiped recess region not including the protrusion-shaped recess region of the mold recess is 1 mm to 3 mm, and the mold recess 4. The mold mold for forming a sample embedding body for microscopic observation according to claim 1, wherein the mold width W2 including the protrusion-shaped concave region is 5 mm to 7 mm.   5. A molded body obtained by filling a mold embedding transparent resin in which a sample is mixed into a mold recess of the sample embedding mold according to claim 1. A sample-embedded body for microscopic observation, characterized in that the sample-embedded body is composed of a shape-depressed region or a rectangular parallelepiped-shaped recessed region and a protrusion-shaped recessed region.   The sample embedding body according to claim 5 is locked and fixed to an observation sample holder of a microscope at a boundary portion between a cubic shaped recessed area or a rectangular parallelepiped shaped recessed area of the embedded body and a projecting shaped recessed area. A method for fixing a sample embedded body for microscopic observation.   The sample embedding body according to claim 5 is configured such that a boundary portion between a cubic-shaped recessed area or a rectangular parallelepiped-shaped recessed area of the embedding body and a protrusion-shaped recessed area is a screwing plate of a sample holder for a screw type microtome of a microscope. A method for fixing a sample-embedded body for microscopic observation, wherein the sample-embedded body for microscopic observation is fixed while being in contact.

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