JP2006006179A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2006006179A5 JP2006006179A5 JP2004187133A JP2004187133A JP2006006179A5 JP 2006006179 A5 JP2006006179 A5 JP 2006006179A5 JP 2004187133 A JP2004187133 A JP 2004187133A JP 2004187133 A JP2004187133 A JP 2004187133A JP 2006006179 A5 JP2006006179 A5 JP 2006006179A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleotide
- nucleotide chain
- modifying
- substance
- chain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000003729 nucleotide group Chemical group 0.000 claims description 67
- 239000002773 nucleotide Substances 0.000 claims description 65
- 238000000034 method Methods 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 13
- 108020004414 DNA Proteins 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 102000035195 Peptidases Human genes 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 125000003172 aldehyde group Chemical group 0.000 claims description 7
- 125000003147 glycosyl group Chemical group 0.000 claims description 7
- 235000019833 protease Nutrition 0.000 claims description 7
- 108020004635 Complementary DNA Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 238000002715 modification method Methods 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 125000003277 amino group Chemical group 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 claims description 2
- 125000000524 functional group Chemical group 0.000 claims 3
- 102000012410 DNA Ligases Human genes 0.000 claims 1
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 102000015636 Oligopeptides Human genes 0.000 claims 1
- 108010038807 Oligopeptides Proteins 0.000 claims 1
- 108091027568 Single-stranded nucleotide Proteins 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 12
- 108020004707 nucleic acids Proteins 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 102000039446 nucleic acids Human genes 0.000 description 11
- FSASIHFSFGAIJM-UHFFFAOYSA-N 3-methyladenine Chemical compound CN1C=NC(N)=C2N=CN=C12 FSASIHFSFGAIJM-UHFFFAOYSA-N 0.000 description 10
- 101000588972 Homo sapiens Myosin-1 Proteins 0.000 description 8
- 102100032975 Myosin-1 Human genes 0.000 description 8
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 239000002262 Schiff base Substances 0.000 description 1
- 150000004753 Schiff bases Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- UFJPAQSLHAGEBL-RRKCRQDMSA-N dITP Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(N=CNC2=O)=C2N=C1 UFJPAQSLHAGEBL-RRKCRQDMSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 150000004662 dithiols Chemical class 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- ZNJHFNUEQDVFCJ-UHFFFAOYSA-M sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OCCN1CCN(CCS(O)(=O)=O)CC1 ZNJHFNUEQDVFCJ-UHFFFAOYSA-M 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004187133A JP4383265B2 (ja) | 2004-06-25 | 2004-06-25 | ヌクレオチド鎖修飾方法 |
| CA2517167A CA2517167C (en) | 2003-06-30 | 2004-06-29 | Method for modifying nucleotide chain |
| EP04746993A EP1647592B1 (en) | 2003-06-30 | 2004-06-29 | Method of modifying nucleotide chain |
| DE602004019926T DE602004019926D1 (de) | 2003-06-30 | 2004-06-29 | Verfahren zur modifizierung einer nukleotidkette |
| KR1020057017735A KR100683025B1 (ko) | 2003-06-30 | 2004-06-29 | 뉴클레오티드쇄 수식방법 |
| AT04746993T ATE425250T1 (de) | 2003-06-30 | 2004-06-29 | Verfahren zur modifizierung einer nukleotidkette |
| US10/554,495 US20070077629A1 (en) | 2003-06-30 | 2004-06-29 | Method for modifying nucleotide chain |
| PCT/JP2004/009524 WO2005014808A1 (ja) | 2003-06-30 | 2004-06-29 | ヌクレオチド鎖修飾方法 |
| US12/720,482 US20100291637A1 (en) | 2003-06-30 | 2010-03-09 | Method for modifying nucleotide chain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004187133A JP4383265B2 (ja) | 2004-06-25 | 2004-06-25 | ヌクレオチド鎖修飾方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006006179A JP2006006179A (ja) | 2006-01-12 |
| JP2006006179A5 true JP2006006179A5 (enExample) | 2006-02-23 |
| JP4383265B2 JP4383265B2 (ja) | 2009-12-16 |
Family
ID=35774092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004187133A Expired - Fee Related JP4383265B2 (ja) | 2003-06-30 | 2004-06-25 | ヌクレオチド鎖修飾方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4383265B2 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5128341B2 (ja) * | 2008-03-31 | 2013-01-23 | 独立行政法人科学技術振興機構 | アルデヒド基を備えるアミジン−カルボン酸ターフェニル会合体とその製法 |
-
2004
- 2004-06-25 JP JP2004187133A patent/JP4383265B2/ja not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2028272B1 (en) | Method of modifying nucleotide chain | |
| US20240318245A1 (en) | Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators | |
| US7078499B2 (en) | Nucleotide analogues comprising a reporter moiety and a polymerase enzyme blocking moiety | |
| JP6382189B2 (ja) | Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット | |
| US11655502B2 (en) | Short pendant arm linkers for nucleotides in sequencing applications | |
| CN107567501A (zh) | 新用途 | |
| JP2003500070A (ja) | 核酸分子を標識するための組成物および方法 | |
| CN102216472A (zh) | Rna检测方法 | |
| EP2231878B1 (en) | A single enzyme system for fast, ultra long pcr | |
| US8691968B2 (en) | Compositions and methods for the protection of nucleophilic groups | |
| CN118804974A (zh) | 多核苷酸合成的方法 | |
| JP2006006179A5 (enExample) | ||
| EP2450453B1 (en) | Method for the replication, amplification or sequencing of a dna template | |
| JP4518754B2 (ja) | ヌクレオチド鎖修飾方法 | |
| EP1647592B1 (en) | Method of modifying nucleotide chain | |
| US20100291637A1 (en) | Method for modifying nucleotide chain | |
| JP4383265B2 (ja) | ヌクレオチド鎖修飾方法 | |
| KR20240024924A (ko) | 폴리머라제 돌연변이체 및 3'-oh 비차단 가역적 종결자와의 사용 | |
| JP2003246794A (ja) | ヌクレオチド鎖修飾方法 | |
| KR20100048028A (ko) | 식물 감염성 세균 또는 곰팡이 검출용 올리고뉴클레오타이드 | |
| CN119040434A (zh) | 一种基于dI修饰引物的单链DNA合成技术 | |
| JP2008199969A (ja) | 膜タンパク質に特異的に結合する機能性分子およびその作製方法 | |
| HK1010209A (en) | Method for enhancing enzyme activity at elevated temperature | |
| JPWO1999002544A1 (ja) | エネルギートランスファー機能を有する化合物及びそれを利用したｄｎａの塩基配列決定方法 |