JP2005514927A5 - - Google Patents

Download PDF

Info

Publication number
JP2005514927A5
JP2005514927A5 JP2003560171A JP2003560171A JP2005514927A5 JP 2005514927 A5 JP2005514927 A5 JP 2005514927A5 JP 2003560171 A JP2003560171 A JP 2003560171A JP 2003560171 A JP2003560171 A JP 2003560171A JP 2005514927 A5 JP2005514927 A5 JP 2005514927A5
Authority
JP
Japan
Prior art keywords
nucleic acid
oligonucleotide
double
library
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
JP2003560171A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005514927A (ja
Filing date
Publication date
Priority claimed from US10/077,474 external-priority patent/US6673552B2/en
Application filed filed Critical
Priority claimed from PCT/US2003/001189 external-priority patent/WO2003060084A2/en
Publication of JP2005514927A publication Critical patent/JP2005514927A/ja
Publication of JP2005514927A5 publication Critical patent/JP2005514927A5/ja
Abandoned legal-status Critical Current

Links

JP2003560171A 2002-01-14 2003-01-14 ポリヌクレオチドの製造方法及び二本鎖ポリヌクレオチドの精製方法 Abandoned JP2005514927A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US34876402P 2002-01-14 2002-01-14
US34860902P 2002-01-14 2002-01-14
US34876102P 2002-01-14 2002-01-14
US10/077,474 US6673552B2 (en) 2002-01-14 2002-02-14 Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
PCT/US2003/001189 WO2003060084A2 (en) 2002-01-14 2003-01-14 Methods for making polynucleotides and purifying double-stranded polynucleotides

Publications (2)

Publication Number Publication Date
JP2005514927A JP2005514927A (ja) 2005-05-26
JP2005514927A5 true JP2005514927A5 (enExample) 2006-03-02

Family

ID=34658146

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003560171A Abandoned JP2005514927A (ja) 2002-01-14 2003-01-14 ポリヌクレオチドの製造方法及び二本鎖ポリヌクレオチドの精製方法

Country Status (6)

Country Link
US (1) US20050130156A1 (enExample)
EP (1) EP1472373A4 (enExample)
JP (1) JP2005514927A (enExample)
AU (1) AU2003217207A1 (enExample)
CA (1) CA2473434A1 (enExample)
WO (1) WO2003060084A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
AU2005295351A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
CN103620032B (zh) * 2010-12-31 2016-02-24 生物蛋白有限公司 抗体的快速人源化
AU2012300401B2 (en) 2011-08-26 2018-02-08 Ginkgo Bioworks, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (en) 2012-04-24 2019-03-06 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20150191719A1 (en) 2012-06-25 2015-07-09 Gen9, Inc. Methods for Nucleic Acid Assembly and High Throughput Sequencing
US9409139B2 (en) 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
AU2016324296A1 (en) 2015-09-18 2018-04-12 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CN110913865A (zh) * 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
KR102637566B1 (ko) 2017-10-20 2024-02-16 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
CN112041438B (zh) 2018-01-04 2025-05-23 特韦斯特生物科学公司 基于dna的数字信息存储
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
JP2022550497A (ja) 2019-06-21 2022-12-02 ツイスト バイオサイエンス コーポレーション バーコードに基づいた核酸配列アセンブリ
CA3155629A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
AU2020355027A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
US6046036A (en) * 1996-10-25 2000-04-04 Advanced Research And Technology Institute DNA sequences encoding fusions of DNA repair proteins and uses thereof
US6333153B1 (en) * 1997-08-28 2001-12-25 Thomas Jefferson University Compositions, kits, and methods for effecting adenine nucleotide modulation of DNA mismatch recognition proteins
US6033861A (en) * 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
US6297010B1 (en) * 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
US7033781B1 (en) * 1999-09-29 2006-04-25 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6365355B1 (en) * 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US6673552B2 (en) * 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps

Similar Documents

Publication Publication Date Title
JP2005514927A5 (enExample)
JP4015193B2 (ja) 反復的選択および組換えにより所望の特徴を有するポリヌクレオチドを作製する方法
JP4067516B2 (ja) ランダムフラグメント化および再組立によるdna変異誘発
JP3344584B2 (ja) 組換えライブラリースクリーニング法
KR101051245B1 (ko) 시험관내 펩타이드 발현 라이브러리
EP1266025B1 (en) Protein scaffolds for antibody mimics and other binding proteins
US20100021477A1 (en) DESIGN AND GENERATION OF HUMAN DE NOVO pIX PHAGE DISPLAY LIBRARIES
JP2000514651A (ja) 合成または増幅過程をブロックまたは中断することにより製造されたポリヌクレオチドによるdnaのシャッフリング方法
JPH06505866A (ja) 結合タンパク質の最適化
JPH08506487A (ja) 全合成親和性試薬
AU2001241850A1 (en) Protein scaffolds for antibody mimics and other binding proteins
WO2010039852A2 (en) Improved antibody libraries
ZA200502624B (en) Method for the synthesis of a bifunctional complex.
JP2003505041A (ja) 固体支持体担体を用いる試験管内選択及びポリペプチドの任意の同定
JP2001514498A (ja) 多数のdna断片の同時連結方法
US5891637A (en) Construction of full length cDNA libraries
US7527954B2 (en) Method for in vitro evolution of polypeptides
KR100491810B1 (ko) 무작위단편화및재조립에의한dna돌연변이유발방법
EP1576154B8 (en) In vivo affinity maturation scheme
AU2013352067A1 (en) Preparation of gene-specific templates for the use in single primer amplification
US20180245070A1 (en) Dna display and methods thereof
WO2025081084A1 (en) Improved methods for determining sequences of nucleic acids
JPH06205691A (ja) モノクローナル抗体の製造方法
AU2003287780A1 (en) In vivo affinity maturation scheme