AU2003217207A1 - Methods for making polynucleotides and purifying double-stranded polynucleotides - Google Patents

Methods for making polynucleotides and purifying double-stranded polynucleotides Download PDF

Info

Publication number
AU2003217207A1
AU2003217207A1 AU2003217207A AU2003217207A AU2003217207A1 AU 2003217207 A1 AU2003217207 A1 AU 2003217207A1 AU 2003217207 A AU2003217207 A AU 2003217207A AU 2003217207 A AU2003217207 A AU 2003217207A AU 2003217207 A1 AU2003217207 A1 AU 2003217207A1
Authority
AU
Australia
Prior art keywords
library
nucleic acid
oligonucleotide
polypeptide
double
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003217207A
Other languages
English (en)
Inventor
Gerhard Frey
Angela Kennedy
Lilian Parra-Gessert
Jay M Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Verenium Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/077,474 external-priority patent/US6673552B2/en
Application filed by Verenium Corp filed Critical Verenium Corp
Publication of AU2003217207A1 publication Critical patent/AU2003217207A1/en
Assigned to VERENIUM CORPORATION reassignment VERENIUM CORPORATION Request for Assignment Assignors: DIVERSA CORPORATION
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003217207A 2002-01-14 2003-01-14 Methods for making polynucleotides and purifying double-stranded polynucleotides Abandoned AU2003217207A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US34876402P 2002-01-14 2002-01-14
US34860902P 2002-01-14 2002-01-14
US34876102P 2002-01-14 2002-01-14
US60/348,764 2002-01-14
US60/348,761 2002-01-14
US60/348,609 2002-01-14
US10/077,474 2002-02-14
US10/077,474 US6673552B2 (en) 2002-01-14 2002-02-14 Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
PCT/US2003/001189 WO2003060084A2 (en) 2002-01-14 2003-01-14 Methods for making polynucleotides and purifying double-stranded polynucleotides

Publications (1)

Publication Number Publication Date
AU2003217207A1 true AU2003217207A1 (en) 2003-07-30

Family

ID=34658146

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003217207A Abandoned AU2003217207A1 (en) 2002-01-14 2003-01-14 Methods for making polynucleotides and purifying double-stranded polynucleotides

Country Status (6)

Country Link
US (1) US20050130156A1 (enExample)
EP (1) EP1472373A4 (enExample)
JP (1) JP2005514927A (enExample)
AU (1) AU2003217207A1 (enExample)
CA (1) CA2473434A1 (enExample)
WO (1) WO2003060084A2 (enExample)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
AU2005295351A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012092374A2 (en) * 2010-12-31 2012-07-05 Short Jay M Express humanization of antibodies
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
AU5195398A (en) * 1996-10-25 1998-05-15 Advanced Research And Technology Institute, Inc. Dna sequences encoding fusions of dna repair proteins and uses thereof
WO1999010369A1 (en) * 1997-08-28 1999-03-04 Thomas Jefferson University Compositions, kits, and methods for effecting adenine nucleotide modulation of dna mismatch recognition proteins
US6033861A (en) * 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
US6297010B1 (en) * 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
US7033781B1 (en) * 1999-09-29 2006-04-25 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6365355B1 (en) * 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US6673552B2 (en) * 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps

Also Published As

Publication number Publication date
JP2005514927A (ja) 2005-05-26
WO2003060084A9 (en) 2004-04-22
EP1472373A2 (en) 2004-11-03
US20050130156A1 (en) 2005-06-16
EP1472373A4 (en) 2005-07-27
WO2003060084A2 (en) 2003-07-24
CA2473434A1 (en) 2003-07-24
WO2003060084A3 (en) 2004-08-19

Similar Documents

Publication Publication Date Title
US20050130156A1 (en) Methods for making polynucleotides and purifying double-stranded polynucleotides
US20040009498A1 (en) Chimeric antigen binding molecules and methods for making and using them
US20030215837A1 (en) Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
US6673552B2 (en) Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
US9255289B2 (en) Geometric patterns and lipid bilayers for DNA molecule organization and uses thereof
JP6626830B2 (ja) Ｄｎａ操作のための複数のトランスポザーゼアダプター
US8137906B2 (en) Method for the synthesis of DNA fragments
US20130059741A1 (en) Binding assays for markers
US20080300842A1 (en) Design, Synthesis and Assembly of Synthetic Nucleic Acids
US20140357500A1 (en) Single cell bar-coding for antibody discovery
JP2005519641A (ja) 標的ポリペプチドをコードするポリヌクレオチドの構築方法
US20070231805A1 (en) Nucleic acid assembly optimization using clamped mismatch binding proteins
JP2004532038A (ja) 新規抗原結合分子の治療、診断、予防、酵素、産業ならびに農業各分野への応用とそのための新規抗原結合分子の作製とスクリーニングの方法
WO1998040519A1 (en) Intron-mediated recombinant techniques and reagents
US20090136986A1 (en) Methods and cells for creating functional diversity and uses thereof
CN104685116A (zh) 用于核酸组装和高通量测序的方法
US20030219781A1 (en) Compositions and methods for making polynucleotides by iterative assembly of codon building blocks
KR20160138579A (ko) 게놈 및 치료학적 적용을 위한 핵산 분자의 클론 복제 및 증폭을 위한 시스템 및 방법
JP2005514927A5 (enExample)
WO2007136833A2 (en) Methods and compositions for aptamer production and uses thereof
IL143123A (en) Methods for the preparation by in vivo recombination of nucleic acid and polypeptide libraries and uses thereof
WO2021053008A1 (en) Immune repertoire profiling by primer extension target enrichment
US20220333178A1 (en) Methods for improving nucleic acid cluster clonality
EP1576154B1 (en) In vivo affinity maturation scheme
CN118984874A (zh) 用于产生具有单链粘端的双链dna片段的异源双链体热稳定性连接组装(htla)和/或环状异源双链体热稳定性连接组装(chtla)

Legal Events

Date Code Title Description
DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: ADD CO-INVENTOR KENNEDY, ANGELA

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: ADD CO-INVENTOR KENNEDY, ANGELA

TC Change of applicant's name (sec. 104)

Owner name: VERENIUM CORPORATION

Free format text: FORMER NAME: DIVERSA CORPORATION

MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application