JP2005281284A - Skin care preparation for external use for promoting collagen synthesis - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use, having collagen synthesis promoting effect, and effectively preventing age-related skin aging. <P>SOLUTION: The biogenic collagen synthesis promotor of the skin care preparation for external use contains a powder, extracts or extracted components containing Coptis japonica and/or at least one selected from berberine, palmatine, coptisine, worenine, jateorrhizine, magnoflorine, ferulic acid and chlorogenic acid as an active component. The biogenic collagen synthesis promotor of the skin care preparation for external use promotor collagen synthesis in vivo, and improves tissue fatigue and hypofunction due to age-related change. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an external preparation for skin that prevents changes associated with aging of the skin by enhancing the ability to promote collagen synthesis in a living body.

  Collagen is a major component of the skin that occupies about 70% of the dermis, and is present in most tissues to protect cells and play an important physiological role such as cell binding as an intercellular factor. Collagen that plays an important role in the living body also decreases with a decrease in metabolism due to aging, which causes factors such as aging, and a countermeasure to improve it is desired. Skin aging is likely to progress and signs of aging are likely to be observed. Skin aging wrinkles and tarmi are caused by a decrease in skin elasticity and flexibility, and are caused by a decrease in collagen.

Until now, as shown in Patent Document 1, a collagen synthesis promoter by oral intake has been developed to some extent. However, as disclosed in Patent Document 2, a technique for inhibiting collagenase to indirectly prevent a decrease in collagen has been disclosed as an external preparation for skin, but there is still no one that can significantly promote collagen synthesis. Very few. Collagen itself can also be blended, but since it is a polymer, there are problems in terms of being difficult to absorb percutaneously and stability.
JP 2003-277286 A JP 2001-316221 A

  An object of the present invention is to provide a collagen synthesis-promoting skin external preparation capable of enhancing the ability to synthesize collagen.

  In the present invention, powders or extracts of oren and / or berberine, palmatin, coptidine, worenine, jateorrhizine, magnoflorine, magnoflorine (magnofluorine) The above-mentioned problems have been solved by incorporating a powder or extract containing at least one selected from acid and chlorogenic acid into a skin external preparation base. The present invention will be described in detail below.

  As described above, the collagen synthesis-promoting skin external preparation of the present invention comprises a powder of aurene, an extract or an extract component and / or berberine, palmatin, coptidine, worenine, jateorridine. ), Magnoflorine, ferulic acid, chlorogenic acid, or a powder, extract or extract component containing at least one selected from chlorogenic acid. With regard to aurene, the use of rhizomes is particularly preferred. Moreover, it is preferable that berberine etc. are extracted from the plant containing these. Examples of plants containing berberine and the like include Auren, Rutaeceae, Phellodendri Cortex, Papaveraceae, Corydalisa, and Ranunculacem. What was concentrated and refine | purified from the extract can also be used.

  What is necessary is just to perform powdering and extraction of the said plant by a conventional method. Extraction can be performed, for example, by drying and chopping the plant, or powdering it, adding an extraction solvent, and cooling or heating. As an extraction solvent, 1 type, or 2 or more types of mixed solvents, such as water, ethanol, 1, 3- butanediol, and isopropanol, can be used.

  The extract in the present invention means any of an extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or an extract. Rough purification and purification of the extract may be performed by a conventional method, and for example, adsorption and elution with an adsorbent such as MCI gel (manufactured by Sigma-Aldrich), chromatography, and the like can be performed in an appropriate combination.

  One or more kinds of powders, extracts, and crude purified products, purified products, and components of the plant obtained as described above are collagen synthesis promoting effects, as will be apparent from Examples described later. Therefore, it can be used as an active ingredient of a skin preparation for promoting collagen synthesis. The collagen synthesis-promoting skin external preparation of the present invention is considered to be highly safe because it is derived from a crude drug as an active ingredient.

  The collagen synthesis-promoting skin external preparation of the present invention can be provided as a quasi-drug or a cosmetic without any problem. The blending amount is suitably about 10% by weight or less in consideration of the effective concentration in the external preparation and the stability of the external preparation. The form of the external preparation can take various forms such as lotion, emulsion, cream, ointment and the like. It can also be provided as a collagen synthesis-promoting skin cosmetic such as skin lotion, cosmetic liquid, and milky lotion.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to these examples. “%” Means% by weight unless otherwise specified.

As an example of the present invention, the formulation of a collagen synthesis promoting skin cream is shown in Table 1. In Table 1, (1) to (5) were mixed and dissolved by heating to 75 ° C., and mixed, heated and dissolved to 75 ° C., and (6) to (9) and (12) were added and emulsified. (10) and (11) are added and mixed at 50 ° C. after stirring and cooling. In Table 1, (10) Ourene extraction fraction solution was prepared by extracting 500 g of Ourene with 5 L of 50% ethanol for 1 week at room temperature and fractionating with MCI gel. Moreover, what mixed ethanol instead of (10) in Table 1 was made into the comparative example.

With respect to the examples of the present invention, the collagen synthesis ability increase test was examined by evaluation using human normal neonatal skin fibroblasts. First, the following (1) 0.5% by volume fetal bovine serum (FBS) -containing Dulbecco's modified basal medium (DMEM), (2) coating buffer, (3) washing buffer, and (4) coloring solution are prepared.
(1) 0.5 volume% FBS-containing DMEM : Prepared by adding a final concentration of 0.5 volume% FBS to DMEM (manufactured by Sigma).
(2) Coating buffer solution : 0.8 g of sodium carbonate, 1.5 g of sodium bicarbonate, 0.1 g of sodium azide / 500 ml of purified water are prepared.
(3) Wash buffer : 0.05 vol% Tween 20 / phosphate buffered saline (PBS (-)) solution.
(4) Coloring solution : 0.1 M aqueous solution of sodium hydrogen phosphate (pH 4.0).

Normal human neonatal dermal fibroblast cell line (NHDF) was prepared at 2 × 10 ⁴ cells / ml in DMEM containing 0.5% by volume FBS, seeded in 100 μl each in a 96-well plate, 5% carbon dioxide gas, saturated water vapor Then, the cells were cultured at 37 ° C.

  After 24 hours, the culture solution was removed by suction, and the plant extract 50% aqueous ethanol solution was added to DMEM containing 0.5% by volume FBS at a maximum concentration of 2 mg / ml (final concentration). Culturing was performed at 37 ° C. under saturated steam.

  48 hours later, 0.5 volume% FBS-containing DMEM: coating buffer = 1: 3 was prepared, culture supernatant was added thereto, mixed, and then added to a 96-well plate for enzyme immunoassay (ELISA) Add 100 μl each. Similarly, a collagen solution is prepared for the calibration curve, and 100 μl is added to the plate. React at 37 ° C. for 2 hours, wash 3 times with wash buffer and block with albumin. Thereafter, the mixture is reacted at 37 ° C. for 1 hour, washed 3 times with a washing buffer, added with 100 μl of primary antibody, reacted at 37 ° C. for 1 hour, and washed with a washing buffer 3 times. Thereafter, the secondary antibody was reacted in the same manner, washed, developed with a coloring reagent, and measured with a microplate reader at 405 nm.

The amount of collagen in the culture supernatant was calculated from the collagen calibration curve, and divided by the amount of protein to obtain the amount of collagen produced. The results are shown in Table 2. As shown below, the amount of collagen produced increased significantly depending on the concentration of the fraction extracted from the aurene.

Subsequently, a use test was performed on the examples and comparative examples of the present invention. When collagen synthesis is promoted, it is assumed that elasticity returns to the skin and it becomes difficult to wrinkle, so the following test was performed. A panel of 15 panelists working outdoors was used as a group, and each group was used for the examples and comparative examples blindly on the face and hands, and changes in wrinkles and skin elasticity were observed and evaluated. The period of use was one year from January to December. For wrinkles, there are four stages: “decrease”, “no change”, “increase in fine wrinkles”, “increase in clear wrinkles”, and skin elasticity “increase”, “no change”, “slightly decrease”, “ Table 3 shows the number of panelists that have obtained the respective evaluations. As shown below, wrinkles were reduced and skin elasticity was remarkably increased by the use of the examples containing the Ouren extract fraction, as compared with the comparative examples containing ethanol.

The invention's effect

  As described above in detail, the collagen synthesis-promoting skin external preparation according to the present invention can promote the biosynthesis of collagen in the skin and can be effective in preventing aging such as wrinkles and tarmi accompanying aging changes. . In addition, since it is an active ingredient derived from herbal medicine, it is a skin external preparation with relatively few side effects and excellent safety.

Claims (3)

  A skin external preparation for promoting collagen production in a living body, comprising a powder, extract or extract component of Ranunculaceae auren (Coptis japonica).   Berberine, palmatin, coptidine, worenine, jateorrhizine, magnoflorine, ferulic acid (1), chlorogenic acid, 1 A skin external preparation for promoting collagen production in a living body, comprising a powder, an extract or an extract component containing two or more kinds.   Powder, extract or extract of auren and berberine, palmatin, coptidine, worenine, jateorrizine, magnoflorine, ferulic acid, ullucic acid A skin external preparation for promoting collagen production in a living body, comprising a powder, an extract or an extract component containing one or more selected from acids (chlorogenic acid).