JP2004525631A5 - - Google Patents
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- Publication number
- JP2004525631A5 JP2004525631A5 JP2002571547A JP2002571547A JP2004525631A5 JP 2004525631 A5 JP2004525631 A5 JP 2004525631A5 JP 2002571547 A JP2002571547 A JP 2002571547A JP 2002571547 A JP2002571547 A JP 2002571547A JP 2004525631 A5 JP2004525631 A5 JP 2004525631A5
- Authority
- JP
- Japan
- Prior art keywords
- buffer
- folding
- complexing agent
- molecular weight
- low molecular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000008139 complexing agent Substances 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 108010067770 Endopeptidase K Proteins 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10105912A DE10105912A1 (de) | 2001-02-09 | 2001-02-09 | Rekombinante Proteinase K |
| PCT/EP2002/001322 WO2002072634A2 (de) | 2001-02-09 | 2002-02-08 | Rekombinante proteinase k |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004525631A JP2004525631A (ja) | 2004-08-26 |
| JP2004525631A5 true JP2004525631A5 (cg-RX-API-DMAC10.html) | 2006-12-07 |
Family
ID=7673417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002571547A Pending JP2004525631A (ja) | 2001-02-09 | 2002-02-08 | 組換えプロテイナーゼｋ |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20070099283A1 (cg-RX-API-DMAC10.html) |
| EP (1) | EP1360284B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP2004525631A (cg-RX-API-DMAC10.html) |
| AT (1) | ATE401393T1 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2435753A1 (cg-RX-API-DMAC10.html) |
| CZ (1) | CZ20032165A3 (cg-RX-API-DMAC10.html) |
| DE (2) | DE10105912A1 (cg-RX-API-DMAC10.html) |
| WO (1) | WO2002072634A2 (cg-RX-API-DMAC10.html) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7153666B2 (en) | 2003-07-17 | 2006-12-26 | General Atomics | Methods and compositions for determination of glycated proteins |
| ES2404059T3 (es) | 2006-07-25 | 2013-05-23 | General Atomics | Procedimientos para analizar el porcentaje de hemoglobina glicada |
| US7943385B2 (en) | 2006-07-25 | 2011-05-17 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
| EP3276349B1 (en) * | 2006-11-01 | 2019-08-07 | Ventana Medical Systems, Inc. | Haptens, hapten conjugates, compositions thereof and method for their preparation and use |
| US8148105B2 (en) * | 2007-03-16 | 2012-04-03 | The Board Of Trustees Of The Leland Stanford Junior University | Scaleable manufacturing process for cysteine endoprotease B, isoform 2 |
| US7682789B2 (en) * | 2007-05-04 | 2010-03-23 | Ventana Medical Systems, Inc. | Method for quantifying biomolecules conjugated to a nanoparticle |
| US7985557B2 (en) | 2007-05-23 | 2011-07-26 | Ventana Medical Systems, Inc. | Polymeric carriers for immunohistochemistry and in situ hybridization |
| EP2283149A1 (en) * | 2008-05-13 | 2011-02-16 | General Atomics | Electrochemical biosensor for direct determination of percentage of glycated hemoglobin |
| CA2979353C (en) | 2008-06-05 | 2020-06-30 | Ventana Medical Systems, Inc. | Compositions comprising nanomaterials and method for using such compositions for histochemical processes |
| USPP22463P3 (en) * | 2010-02-16 | 2012-01-17 | Menachem Bornstein | Gypsophila plant named ‘Pearl Blossom’ |
| EP2423217A1 (en) | 2010-08-23 | 2012-02-29 | Forschungsverbund Berlin E.V. | Peptide and protein affinity tag from mistic protein |
| CN112592931A (zh) * | 2020-12-31 | 2021-04-02 | 安徽丰原发酵技术工程研究有限公司 | 一种生产重组蛋白酶k的方法 |
| CN118165964B (zh) * | 2024-04-11 | 2025-01-24 | 铭诚惠众(江苏)药物研究有限公司 | 一种重组蛋白酶k的纯化方法及其应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5332805A (en) * | 1984-02-03 | 1994-07-26 | Celltech Limited | Process for the recovery of recombinantly produced chymosin from insoluble aggregate |
| US5082775A (en) * | 1984-05-11 | 1992-01-21 | Berlex Laboratories, Inc. | Efficient process for isolating insoluble heterologous protein using non-ionic detergents |
| US5453363A (en) * | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
| DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| ATE139567T1 (de) * | 1987-04-03 | 1996-07-15 | Amgen Inc | Proteolytische enzyme |
| DE3835350A1 (de) * | 1988-10-17 | 1990-04-19 | Boehringer Mannheim Gmbh | Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern |
| DE3903581A1 (de) * | 1989-02-07 | 1990-08-16 | Boehringer Mannheim Gmbh | Gewebs-plasminogenaktivator-derivat |
| US5191063A (en) * | 1989-05-02 | 1993-03-02 | University Of Medicine And Dentistry Of New Jersey | Production of biologically active polypeptides by treatment with an exogenous peptide sequence |
| US5606031A (en) * | 1990-04-06 | 1997-02-25 | Lile; Jack | Production and purification of biologically active recombinant neurotrophic protein in bacteria |
| US5144006A (en) * | 1991-06-13 | 1992-09-01 | The Rockefeller University | Oxidative folding of peptide and protein substrates using hydrocarbon sulfoxides |
| US5792623A (en) * | 1992-04-06 | 1998-08-11 | Immuno Aktiengesellschaft | Method for producing activated blood factors with a protease and a detergent |
| DE4225427A1 (de) * | 1992-07-31 | 1994-02-03 | Boehringer Mannheim Gmbh | Verfahren zur Gewinnung rekombinanter, biologisch aktiver, eukaryontischer alkalischer Phosphatase |
| NZ261571A (en) * | 1993-02-04 | 1997-03-24 | Denzyme Aps | Producing correctly folded proteins using multiple denaturation and renaturation cycles |
| US5747654A (en) * | 1993-06-14 | 1998-05-05 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant disulfide-stabilized polypeptide fragments having binding specificity |
| JPH0748398A (ja) * | 1993-08-03 | 1995-02-21 | Nippon Oil Co Ltd | 変性蛋白質の再生方法及び変性蛋白質再生剤 |
| US5550262A (en) * | 1994-11-14 | 1996-08-27 | Cephalon, Inc. | Multicatalytic protease inhibitors |
| IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
| AU714318B2 (en) * | 1996-06-11 | 2000-01-06 | Roche Diagnostics Gmbh | Process for the activation of denatured protein |
| US6426075B1 (en) * | 1996-11-06 | 2002-07-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Protease-activatable pseudomonas exotoxin A-like proproteins |
| US20030207402A1 (en) * | 1997-08-22 | 2003-11-06 | Erhard Kopetzki | Autocatalytically activatable zymogenic precursors of proteases and their use |
| WO2000034504A2 (en) * | 1998-12-10 | 2000-06-15 | Genetic Vectors, Inc. | Method of protein removal |
| US7351549B2 (en) * | 2000-01-24 | 2008-04-01 | Polymun Scientific Immunbiologische Forschung Gmbh | Method for the manufacture of recombinant trypsin |
| US7001724B1 (en) * | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| JP3878132B2 (ja) * | 2000-12-15 | 2007-02-07 | ベーリンガー インゲルハイム （カナダ） リミテッド | 精製活性型ｈｃｖｎｓ２／３プロテアーゼ |
| US7214484B2 (en) * | 2002-12-17 | 2007-05-08 | Sigma-Aldrich Co. | Compositions and methods for nucleic acid extraction from biological samples |
-
2001
- 2001-02-09 DE DE10105912A patent/DE10105912A1/de not_active Withdrawn
-
2002
- 2002-02-08 DE DE50212510T patent/DE50212510D1/de not_active Expired - Fee Related
- 2002-02-08 CA CA002435753A patent/CA2435753A1/en not_active Abandoned
- 2002-02-08 CZ CZ20032165A patent/CZ20032165A3/cs unknown
- 2002-02-08 JP JP2002571547A patent/JP2004525631A/ja active Pending
- 2002-02-08 AT AT02750504T patent/ATE401393T1/de not_active IP Right Cessation
- 2002-02-08 WO PCT/EP2002/001322 patent/WO2002072634A2/de not_active Ceased
- 2002-02-08 EP EP02750504A patent/EP1360284B1/de not_active Expired - Lifetime
- 2002-02-08 US US10/467,532 patent/US20070099283A1/en not_active Abandoned
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