DE10105912A1 - Rekombinante Proteinase K - Google Patents

Rekombinante Proteinase K

Info

Publication number
DE10105912A1
DE10105912A1 DE10105912A DE10105912A DE10105912A1 DE 10105912 A1 DE10105912 A1 DE 10105912A1 DE 10105912 A DE10105912 A DE 10105912A DE 10105912 A DE10105912 A DE 10105912A DE 10105912 A1 DE10105912 A1 DE 10105912A1
Authority
DE
Germany
Prior art keywords
proteinase
recombinant
concentration
buffer
zymogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE10105912A
Other languages
German (de)
English (en)
Inventor
Rainer Mueller
Helmut Schoen
Rainer Schmuck
Frank Geipel
Thomas Meier
Stephan Glaser
Bernhard Rexer
Johann-Peter Thalhofer
Bjoern Schott
Rainer Rudolph
Hauke Lilie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE10105912A priority Critical patent/DE10105912A1/de
Priority to US10/467,532 priority patent/US20070099283A1/en
Priority to AT02750504T priority patent/ATE401393T1/de
Priority to DE50212510T priority patent/DE50212510D1/de
Priority to EP02750504A priority patent/EP1360284B1/de
Priority to JP2002571547A priority patent/JP2004525631A/ja
Priority to CA002435753A priority patent/CA2435753A1/en
Priority to PCT/EP2002/001322 priority patent/WO2002072634A2/de
Priority to CZ20032165A priority patent/CZ20032165A3/cs
Publication of DE10105912A1 publication Critical patent/DE10105912A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21064—Peptidase K (3.4.21.64)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE10105912A 2001-02-09 2001-02-09 Rekombinante Proteinase K Withdrawn DE10105912A1 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE10105912A DE10105912A1 (de) 2001-02-09 2001-02-09 Rekombinante Proteinase K
US10/467,532 US20070099283A1 (en) 2001-02-09 2002-02-08 Recombinant proteinase k
AT02750504T ATE401393T1 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k
DE50212510T DE50212510D1 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k
EP02750504A EP1360284B1 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k
JP2002571547A JP2004525631A (ja) 2001-02-09 2002-02-08 組換えプロテイナーゼｋ
CA002435753A CA2435753A1 (en) 2001-02-09 2002-02-08 Renaturation and activation of the proteinase k zmyogen produced in inclusion bodies
PCT/EP2002/001322 WO2002072634A2 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k
CZ20032165A CZ20032165A3 (cs) 2001-02-09 2002-02-08 Rekombinantní proteináza K

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10105912A DE10105912A1 (de) 2001-02-09 2001-02-09 Rekombinante Proteinase K

Publications (1)

Publication Number Publication Date
DE10105912A1 true DE10105912A1 (de) 2002-08-14

Family

ID=7673417

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10105912A Withdrawn DE10105912A1 (de) 2001-02-09 2001-02-09 Rekombinante Proteinase K
DE50212510T Expired - Fee Related DE50212510D1 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE50212510T Expired - Fee Related DE50212510D1 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k

Country Status (8)

Country Link
US (1) US20070099283A1 (cg-RX-API-DMAC10.html)
EP (1) EP1360284B1 (cg-RX-API-DMAC10.html)
JP (1) JP2004525631A (cg-RX-API-DMAC10.html)
AT (1) ATE401393T1 (cg-RX-API-DMAC10.html)
CA (1) CA2435753A1 (cg-RX-API-DMAC10.html)
CZ (1) CZ20032165A3 (cg-RX-API-DMAC10.html)
DE (2) DE10105912A1 (cg-RX-API-DMAC10.html)
WO (1) WO2002072634A2 (cg-RX-API-DMAC10.html)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153666B2 (en) 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
ES2404059T3 (es) 2006-07-25 2013-05-23 General Atomics Procedimientos para analizar el porcentaje de hemoglobina glicada
US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
EP3276349B1 (en) * 2006-11-01 2019-08-07 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US8148105B2 (en) * 2007-03-16 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Scaleable manufacturing process for cysteine endoprotease B, isoform 2
US7682789B2 (en) * 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
US7985557B2 (en) 2007-05-23 2011-07-26 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
EP2283149A1 (en) * 2008-05-13 2011-02-16 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
CA2979353C (en) 2008-06-05 2020-06-30 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
USPP22463P3 (en) * 2010-02-16 2012-01-17 Menachem Bornstein Gypsophila plant named ‘Pearl Blossom’
EP2423217A1 (en) 2010-08-23 2012-02-29 Forschungsverbund Berlin E.V. Peptide and protein affinity tag from mistic protein
CN112592931A (zh) * 2020-12-31 2021-04-02 安徽丰原发酵技术工程研究有限公司 一种生产重组蛋白酶k的方法
CN118165964B (zh) * 2024-04-11 2025-01-24 铭诚惠众(江苏)药物研究有限公司 一种重组蛋白酶k的纯化方法及其应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
ATE139567T1 (de) * 1987-04-03 1996-07-15 Amgen Inc Proteolytische enzyme
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US5606031A (en) * 1990-04-06 1997-02-25 Lile; Jack Production and purification of biologically active recombinant neurotrophic protein in bacteria
US5144006A (en) * 1991-06-13 1992-09-01 The Rockefeller University Oxidative folding of peptide and protein substrates using hydrocarbon sulfoxides
US5792623A (en) * 1992-04-06 1998-08-11 Immuno Aktiengesellschaft Method for producing activated blood factors with a protease and a detergent
DE4225427A1 (de) * 1992-07-31 1994-02-03 Boehringer Mannheim Gmbh Verfahren zur Gewinnung rekombinanter, biologisch aktiver, eukaryontischer alkalischer Phosphatase
NZ261571A (en) * 1993-02-04 1997-03-24 Denzyme Aps Producing correctly folded proteins using multiple denaturation and renaturation cycles
US5747654A (en) * 1993-06-14 1998-05-05 The United States Of America As Represented By The Department Of Health And Human Services Recombinant disulfide-stabilized polypeptide fragments having binding specificity
JPH0748398A (ja) * 1993-08-03 1995-02-21 Nippon Oil Co Ltd 変性蛋白質の再生方法及び変性蛋白質再生剤
US5550262A (en) * 1994-11-14 1996-08-27 Cephalon, Inc. Multicatalytic protease inhibitors
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
AU714318B2 (en) * 1996-06-11 2000-01-06 Roche Diagnostics Gmbh Process for the activation of denatured protein
US6426075B1 (en) * 1996-11-06 2002-07-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Protease-activatable pseudomonas exotoxin A-like proproteins
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
WO2000034504A2 (en) * 1998-12-10 2000-06-15 Genetic Vectors, Inc. Method of protein removal
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
US7001724B1 (en) * 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
JP3878132B2 (ja) * 2000-12-15 2007-02-07 ベーリンガー インゲルハイム （カナダ） リミテッド 精製活性型ｈｃｖｎｓ２／３プロテアーゼ
US7214484B2 (en) * 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples

Also Published As

Publication number Publication date
CZ20032165A3 (cs) 2004-10-13
JP2004525631A (ja) 2004-08-26
ATE401393T1 (de) 2008-08-15
EP1360284A2 (de) 2003-11-12
US20070099283A1 (en) 2007-05-03
EP1360284B1 (de) 2008-07-16
WO2002072634A2 (de) 2002-09-19
CA2435753A1 (en) 2002-09-19
DE50212510D1 (de) 2008-08-28
WO2002072634A3 (de) 2003-03-13

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Legal Events

Date Code Title Description
8141 Disposal/no request for examination