JP2004501611A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2004501611A5 JP2004501611A5 JP2001569326A JP2001569326A JP2004501611A5 JP 2004501611 A5 JP2004501611 A5 JP 2004501611A5 JP 2001569326 A JP2001569326 A JP 2001569326A JP 2001569326 A JP2001569326 A JP 2001569326A JP 2004501611 A5 JP2004501611 A5 JP 2004501611A5
- Authority
- JP
- Japan
- Prior art keywords
- composition
- phosphatase
- nuclease
- present
- effective amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101710163270 Nuclease Proteins 0.000 claims 11
- 238000000034 method Methods 0.000 claims 9
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 7
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 7
- 239000000126 substance Substances 0.000 claims 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims 3
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims 3
- 238000006243 chemical reaction Methods 0.000 claims 3
- 230000005714 functional activity Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 239000000047 product Substances 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- 241000238557 Decapoda Species 0.000 claims 2
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 claims 2
- 102100029075 Exonuclease 1 Human genes 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 239000007795 chemical reaction product Substances 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- -1 nucleotide triphosphates Chemical class 0.000 claims 2
- 238000003786 synthesis reaction Methods 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 238000001712 DNA sequencing Methods 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 238000012408 PCR amplification Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000006172 buffering agent Substances 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 238000001668 nucleic acid synthesis Methods 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19081300P | 2000-03-21 | 2000-03-21 | |
| PCT/US2001/008859 WO2001070943A2 (en) | 2000-03-21 | 2001-03-20 | Stable composition comprising a nuclease and a phosphatase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004501611A JP2004501611A (ja) | 2004-01-22 |
| JP2004501611A5 true JP2004501611A5 (cg-RX-API-DMAC10.html) | 2005-01-27 |
| JP3803295B2 JP3803295B2 (ja) | 2006-08-02 |
Family
ID=22702898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001569326A Expired - Lifetime JP3803295B2 (ja) | 2000-03-21 | 2001-03-20 | ヌクレアーゼ及びホスファターゼを含有する安定組成物 |
Country Status (6)
| Country | Link |
|---|---|
| EP (2) | EP2803724A1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP3803295B2 (cg-RX-API-DMAC10.html) |
| AU (1) | AU2001250890A1 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2403438C (cg-RX-API-DMAC10.html) |
| NO (1) | NO20024537L (cg-RX-API-DMAC10.html) |
| WO (1) | WO2001070943A2 (cg-RX-API-DMAC10.html) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005095648A1 (ja) * | 2004-04-01 | 2005-10-13 | Riken | ウシ主要組織適合遺伝子複合体(BoLA)クラスII DQAの対立遺伝子決定法 |
| DE102009012039A1 (de) * | 2009-03-10 | 2010-09-16 | Qiagen Gmbh | Quantifizierung von Nukleinsäuren |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756285A (en) | 1991-09-27 | 1998-05-26 | Amersham Life Science, Inc. | DNA cycle sequencing |
| US5674679A (en) | 1991-09-27 | 1997-10-07 | Amersham Life Science, Inc. | DNA cycle sequencing |
| US6458541B1 (en) * | 1999-08-11 | 2002-10-01 | Whitehead Institute For Biomedical Research | BDNF polymorphism and association with bipolar disorder |
-
2001
- 2001-03-20 WO PCT/US2001/008859 patent/WO2001070943A2/en not_active Ceased
- 2001-03-20 AU AU2001250890A patent/AU2001250890A1/en not_active Abandoned
- 2001-03-20 CA CA002403438A patent/CA2403438C/en not_active Expired - Lifetime
- 2001-03-20 EP EP14173528.2A patent/EP2803724A1/en not_active Withdrawn
- 2001-03-20 JP JP2001569326A patent/JP3803295B2/ja not_active Expired - Lifetime
- 2001-03-20 EP EP01924218A patent/EP1343877A2/en not_active Ceased
-
2002
- 2002-09-20 NO NO20024537A patent/NO20024537L/no not_active Application Discontinuation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2600758C (en) | Compositions and method for storage of nucleic acid from bodily fluids | |
| USRE49207E1 (en) | Transposase-random priming DNA sample preparation | |
| EP2272952B8 (en) | Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity | |
| EP1050587A3 (en) | Generation and amplification of nucleic acids from ribonucleic acids | |
| EP3571318A1 (en) | Method for making an asymmetrically-tagged sequencing library | |
| WO2007149789A2 (en) | Conversion of target specific amplification to universal sequencing | |
| DK0721988T3 (da) | Kimærisk oligonucleotid og dets anvendelse ved opnåelse af transkripter af en nucleinsyre | |
| ATE396280T1 (de) | Detektion und amplifizierung von rna unterverwendung von zielgerichteter dna-ligation durch rna ligase | |
| KR930700680A (ko) | Rna 레플리카제의 dna-의존성 rna 폴리머라제 활성을 이용한 핵산 증폭 | |
| WO2001092569A3 (en) | Formulation for polymerase chain reaction and vessel containing same | |
| US20190169603A1 (en) | Compositions and Methods for Labeling Target Nucleic Acid Molecules | |
| EP2964778A1 (en) | Detection of genomic rearrangements by sequence capture | |
| ATE403747T1 (de) | Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten | |
| Dissing et al. | Exploring the limits for the survival of DNA in blood stains | |
| CN112522370A (zh) | 一种核酸免提取试剂及其在核酸检测中的应用和使用方法 | |
| ATE346169T1 (de) | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen | |
| JP2005518217A5 (cg-RX-API-DMAC10.html) | ||
| JP2007520236A (ja) | 抗凍結タンパク質により高められた核酸増幅 | |
| WO2002101004A3 (en) | Low-temperature cycle extension of dna with high priming specificity | |
| JP2013515458A5 (cg-RX-API-DMAC10.html) | ||
| WO2007002031A3 (en) | Dna polymerase blends and mutant dna polymerases | |
| JPH1080280A (ja) | 核酸合成法 | |
| JP2023543602A (ja) | 標的化された配列付加 | |
| WO2021262013A1 (en) | Bst-nec dna fusion polymerase for use in isothermal replication of specific sars cov-2 virus sequences | |
| US20240401029A1 (en) | Pre-library depletion of nucleic acids |