JP2004502130A - 等温変性下で標的種に結合する化合物の同定方法 - Google Patents

等温変性下で標的種に結合する化合物の同定方法 Download PDF

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Publication number
JP2004502130A
JP2004502130A JP2001547549A JP2001547549A JP2004502130A JP 2004502130 A JP2004502130 A JP 2004502130A JP 2001547549 A JP2001547549 A JP 2001547549A JP 2001547549 A JP2001547549 A JP 2001547549A JP 2004502130 A JP2004502130 A JP 2004502130A
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Japan
Prior art keywords
denaturation
target species
protein
test
test compound
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Pending
Application number
JP2001547549A
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English (en)
Japanese (ja)
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JP2004502130A5 (enExample
Inventor
トミク，ポール　ケー．
エップス，デニス　イー．
ケズディー，フェレンク　ジェイ．
マーシュク，チャールズ　ケー．
サーバー，ロナルド　ダブリュ．
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Pharmacia and Upjohn Co
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Pharmacia and Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia and Upjohn Co filed Critical Pharmacia and Upjohn Co
Publication of JP2004502130A publication Critical patent/JP2004502130A/ja
Publication of JP2004502130A5 publication Critical patent/JP2004502130A5/ja
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/115831—Condition or time responsive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2001547549A 1999-12-09 2000-12-07 等温変性下で標的種に結合する化合物の同定方法 Pending JP2004502130A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/458,171 US6376180B1 (en) 1999-12-09 1999-12-09 Methods of identifying compounds that bind to target species under isothermal denaturing conditions
PCT/US2000/042677 WO2001046693A2 (en) 1999-12-09 2000-12-07 Screening under isothermal denaturing conditions

Publications (2)

Publication Number Publication Date
JP2004502130A true JP2004502130A (ja) 2004-01-22
JP2004502130A5 JP2004502130A5 (enExample) 2005-12-22

Family

ID=23819666

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001547549A Pending JP2004502130A (ja) 1999-12-09 2000-12-07 等温変性下で標的種に結合する化合物の同定方法

Country Status (7)

Country Link
US (2) US6376180B1 (enExample)
EP (1) EP1364213A2 (enExample)
JP (1) JP2004502130A (enExample)
AU (1) AU775000B2 (enExample)
CA (1) CA2392777A1 (enExample)
TW (1) TWI240073B (enExample)
WO (1) WO2001046693A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006010491A (ja) * 2004-06-25 2006-01-12 Mitsubishi Kagaku Bio-Clinical Laboratories Inc 体液分析方法及び分注良否判定装置
JP2014513293A (ja) * 2011-04-18 2014-05-29 エビトラプロテオマ アーベー サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2203832A1 (en) * 1995-09-08 1997-03-13 Jaime E. Arenas Screen for compounds with affinity for rna
WO1999024050A1 (en) 1997-11-12 1999-05-20 3-Dimensional Pharmaceuticals, Inc. High throughput method for functionally classifying proteins identified using a genomics approach
US6569631B1 (en) * 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
US6582907B1 (en) * 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
EP1274983B1 (en) * 2000-03-21 2012-02-01 The Board Of Trustees Of The University Of Illinois Colorimetric artificial nose having an array of dyes and method for artificial olfaction
WO2002103321A2 (en) * 2001-06-14 2002-12-27 Anadys Pharmaceuticals, Inc. Methods of screening for ligands of target molecules
US7672786B2 (en) * 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
EP3183580B1 (en) * 2014-08-21 2025-03-26 Eurofins DiscoverX Corporation Homogenous thermal shift ligand binding assay
EP4455668A3 (en) 2015-02-05 2025-02-19 Promega Corporation Luciferase-based thermal shift assays
CN109444209A (zh) * 2018-10-26 2019-03-08 北京食品科学研究院 一种测量蛋清粉玻璃化转变温度的方法
CN110095421B (zh) * 2019-05-17 2022-02-08 淮海工学院 一种胶原蛋白变性温度的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3939350A (en) * 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US5585277A (en) 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
CA2184195C (en) * 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
WO1997020952A1 (en) * 1995-12-07 1997-06-12 Scriptgen Pharmaceuticals, Inc. A fluorescence-based screening method for identifying ligands
US6036920A (en) 1996-05-09 2000-03-14 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
EP0836090A1 (en) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Method of analysis of samples by determination of the distribution of specific brightnesses of particles
PT1036192E (pt) * 1997-12-05 2003-06-30 Upjohn Co Ensaios de pesquisa de alto rendimento a base de fluorescencia para proteina-quinases e -fosfatases
US6242190B1 (en) * 1999-12-01 2001-06-05 John Hopkins University Method for high throughput thermodynamic screening of ligands

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006010491A (ja) * 2004-06-25 2006-01-12 Mitsubishi Kagaku Bio-Clinical Laboratories Inc 体液分析方法及び分注良否判定装置
JP2014513293A (ja) * 2011-04-18 2014-05-29 エビトラプロテオマ アーベー サーマルシフトアッセイを使用した、標的タンパク質とのリガンド結合を決定するための方法

Also Published As

Publication number Publication date
TWI240073B (en) 2005-09-21
US6376180B1 (en) 2002-04-23
AU775000B2 (en) 2004-07-15
CA2392777A1 (en) 2001-06-28
US20020068298A1 (en) 2002-06-06
EP1364213A2 (en) 2003-11-26
AU4713701A (en) 2001-07-03
WO2001046693A3 (en) 2003-09-12
US6713260B2 (en) 2004-03-30
WO2001046693A2 (en) 2001-06-28

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