JP2004187607A - 核酸増幅産物に関する情報を得る方法 - Google Patents
核酸増幅産物に関する情報を得る方法 Download PDFInfo
- Publication number
- JP2004187607A JP2004187607A JP2002361157A JP2002361157A JP2004187607A JP 2004187607 A JP2004187607 A JP 2004187607A JP 2002361157 A JP2002361157 A JP 2002361157A JP 2002361157 A JP2002361157 A JP 2002361157A JP 2004187607 A JP2004187607 A JP 2004187607A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- amplification product
- amplification
- obtaining information
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 191
- 230000003321 amplification Effects 0.000 title claims abstract description 187
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 107
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 107
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 79
- 239000000126 substance Substances 0.000 claims abstract description 52
- 238000002372 labelling Methods 0.000 claims description 25
- 239000000138 intercalating agent Substances 0.000 claims description 22
- 238000004458 analytical method Methods 0.000 claims description 16
- 230000035772 mutation Effects 0.000 claims description 15
- 238000005315 distribution function Methods 0.000 claims description 5
- 238000012921 fluorescence analysis Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 description 84
- 239000000047 product Substances 0.000 description 74
- 239000000523 sample Substances 0.000 description 70
- 239000002773 nucleotide Substances 0.000 description 34
- 125000003729 nucleotide group Chemical group 0.000 description 34
- 238000005259 measurement Methods 0.000 description 25
- 238000001514 detection method Methods 0.000 description 13
- 239000007795 chemical reaction product Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000009396 hybridization Methods 0.000 description 9
- 230000003287 optical effect Effects 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 238000007397 LAMP assay Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000005311 autocorrelation function Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 description 4
- 102000054765 polymorphisms of proteins Human genes 0.000 description 4
- 230000007423 decrease Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 230000005653 Brownian motion process Effects 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000005537 brownian motion Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 230000015654 memory Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- UIZLQMLDSWKZGC-UHFFFAOYSA-N cadmium helium Chemical compound [He].[Cd] UIZLQMLDSWKZGC-UHFFFAOYSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005100 correlation spectroscopy Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000475 fluorescence cross-correlation spectroscopy Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- CPBQJMYROZQQJC-UHFFFAOYSA-N helium neon Chemical compound [He].[Ne] CPBQJMYROZQQJC-UHFFFAOYSA-N 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 229910052743 krypton Inorganic materials 0.000 description 1
- DNNSSWSSYDEUBZ-UHFFFAOYSA-N krypton atom Chemical compound [Kr] DNNSSWSSYDEUBZ-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003541 multi-stage reaction Methods 0.000 description 1
- 238000011330 nucleic acid test Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002361157A JP2004187607A (ja) | 2002-12-12 | 2002-12-12 | 核酸増幅産物に関する情報を得る方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002361157A JP2004187607A (ja) | 2002-12-12 | 2002-12-12 | 核酸増幅産物に関する情報を得る方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004187607A true JP2004187607A (ja) | 2004-07-08 |
| JP2004187607A5 JP2004187607A5 (https=) | 2006-02-02 |
Family
ID=32760007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002361157A Pending JP2004187607A (ja) | 2002-12-12 | 2002-12-12 | 核酸増幅産物に関する情報を得る方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2004187607A (https=) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2006054690A1 (ja) * | 2004-11-19 | 2008-06-05 | 株式会社島津製作所 | 遺伝子多型検出方法、診断方法、並びにそのための装置及び検査試薬キット |
| WO2013021687A1 (ja) * | 2011-08-11 | 2013-02-14 | オリンパス株式会社 | 標的粒子の検出方法 |
| JP2014079260A (ja) * | 2011-09-14 | 2014-05-08 | Ngk Insulators Ltd | 標的核酸の検出方法 |
| JPWO2013038534A1 (ja) * | 2011-09-14 | 2015-03-23 | 日本碍子株式会社 | 標的核酸の検出方法 |
| US9428796B2 (en) | 2012-02-22 | 2016-08-30 | Olympus Corporation | Method for detecting a target particle |
| US9771612B2 (en) | 2012-03-21 | 2017-09-26 | Olympus Corporation | Method for detecting a target nucleic acid molecule |
| US9841418B2 (en) | 2011-08-30 | 2017-12-12 | Olympus Corporation | Method for detecting target particle |
-
2002
- 2002-12-12 JP JP2002361157A patent/JP2004187607A/ja active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2006054690A1 (ja) * | 2004-11-19 | 2008-06-05 | 株式会社島津製作所 | 遺伝子多型検出方法、診断方法、並びにそのための装置及び検査試薬キット |
| JP4742050B2 (ja) * | 2004-11-19 | 2011-08-10 | 株式会社島津製作所 | 検査試薬キットとそれを用いる遺伝子多型検出装置 |
| WO2013021687A1 (ja) * | 2011-08-11 | 2013-02-14 | オリンパス株式会社 | 標的粒子の検出方法 |
| JPWO2013021687A1 (ja) * | 2011-08-11 | 2015-03-05 | オリンパス株式会社 | 標的粒子の検出方法 |
| US9354176B2 (en) | 2011-08-11 | 2016-05-31 | Olympus Corporation | Method for detecting a target particle |
| US9841418B2 (en) | 2011-08-30 | 2017-12-12 | Olympus Corporation | Method for detecting target particle |
| JP2014079260A (ja) * | 2011-09-14 | 2014-05-08 | Ngk Insulators Ltd | 標的核酸の検出方法 |
| JPWO2013038534A1 (ja) * | 2011-09-14 | 2015-03-23 | 日本碍子株式会社 | 標的核酸の検出方法 |
| JPWO2013039228A1 (ja) * | 2011-09-14 | 2015-03-26 | 日本碍子株式会社 | 標的核酸の検出方法 |
| US9428796B2 (en) | 2012-02-22 | 2016-08-30 | Olympus Corporation | Method for detecting a target particle |
| US9771612B2 (en) | 2012-03-21 | 2017-09-26 | Olympus Corporation | Method for detecting a target nucleic acid molecule |
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|---|---|---|---|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20051212 |
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