JP2004125631A - Versatile flash-less embedding dish, cassette of the same and embedding method when embedding block of pathology texture sample cassette is prepared - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To prevent a flash of an embedding agent from occurring on a periphery between a pathology sample cassette and an opening (2a) of an embedding dish (59) when the embedding agent is cured and fills the pathology sample cassette placed on the periphery of the opening (2a) of the embedding dish (59). <P>SOLUTION: A water tight frame (14) for water-tightly fitting to outer faces of a quadrangular pathology sample cassette integrally and outwardly protrudes from the periphery of the opening (2a) of the pathology sample flash-less embedding dish (59). <P>COPYRIGHT: (C)2004,JPO

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention provides a method for diagnosing a disease and deciding a course of treatment for a pathological tissue sample obtained by slicing a pathological tissue sample collected from a patient into a stained sample and returning it to the clinician. The present invention relates to an embedding dish for molding an embedding block of the pathological sample used in the management system of (1).
[0002]
In addition, even when a conventional cassette other than the cassette for pathological tissue samples without burrs of Japanese Patent Application No. 2001-105509 according to the inventor's prior application is used, embedding of the pathological tissue sample in a state before the thin section of the pathological tissue sample is performed. When forming a block, it is intended to prevent burrs from being generated in the embedding block, to make the thickness of the section at the time of slicing uniform, to improve the accuracy of pathological examination, and to improve the conventional embedding. In the working process, burrs are generated around the conventional cassette due to the structure of the embedding dish, and the burrs are removed with a knife or heat. Eliminating and streamlining the deburring work, and embedding the damaged surface of the sample data printed around the cassette, which occurs during the deburring work of the conventional cassette by this conventional embedding dish embedding method This prevents the reading of sample data from becoming difficult after the block is manufactured.
[0003]
[Prior art]
Conventionally, in this type of work, a pathological tissue sample collected from each patient by a clinician is stored in a container with a label or the like on which each patient name or other data is described, and each patient is These attributes are combined with an examination request form in which clinical diagnoses, findings, and the like are described, and are transported by appropriate means such as mailing or messenger.
[0004]
Reception and entry of patient specimens before specimen preparation work for the pathologist to make a pathological diagnosis of each patient sample stored in the above-mentioned container transferred to the pathological examination side at a location away from the clinician The pathological diagnosis test request, which is sometimes attached together with the patient sample container, is checked against the name of each patient, the contents of the request, the status of the pathological tissue sample, etc. Make sure it is.
[0005]
Then, at the time of reception / entry, the clinician's patient ID number and chart number at the time of collection of histopathological tissue samples, as well as the pathological tissue sample collation random number at the time of collection and delivery of patient samples or messenger sample reception. No, another cassette with the patient's sample number newly written in a serial specification number or sample number (one-touch on the microtome with the sample processing basket and sample embedding block frame base developed in the United States more than 30 years ago) For systematization that can be attached and detached, the external method for fitting to the adapter is about 40 x 28 x 6 mm, and the locking claw of the adapter fits in the position of this external method and the cassette frame shape The base is fixed. All cassettes have this position.) The above-mentioned pathological sample of the patient trimmed is stored therein, and then a chemical solution for preparing a specimen is stored. The embedding block is formed after all the samples are picked up with tweezers, etc., transferred to the bottom of the embedding dish, embedded with an embedding medium, attached to a stock (base), and coagulated. Excess burrs of the embedding agent formed on the base around the base are removed and eliminated to complete an embedded block sample.
[0006]
In the prior application of Japanese Patent Application No. 2001-105509, the burrs-free cassette for pathological tissue samples and the embedding method using the embedding dish, an opening is formed on the upper side, which has been filed by the inventor. By using a cassette bottom consisting of a frame-shaped substrate with a porous support bottom plate projecting with a smaller area than the outer peripheral surface on the side on the side, and using a dedicated burr-free embedding dish together, it can. However, many conventional cassettes do not have a porous support bottom plate projecting with an area smaller than the outer peripheral surface of the bottom surface, and many printers for printing sample numbers (serial specification numbers and sample numbers) of cassettes conform to the shape. Made for use. Of course, it is possible to make a print printer for the cassette bottom with a protruding porous support bottom plate, or to devise the opening and the porous support bottom plate to suit the use of existing print printers. There are no burr-free embedding dishes using conventional cassette bottoms to suit use.
[0007]
The block sample is sliced, the section is stretched and attached to a slide glass, dried, and then unsealable (deparaffinized), stained and sealed to prepare a stained slide specimen. The pathological diagnosis is performed by collating the sample data of the patient with the request form data and the medical history data, and the pathological diagnosis is reported to the clinician.
[0008]
[Problems to be solved by the invention]
An object of the present invention is to provide a clinician with the work of collecting a pathological tissue sample including a biopsy sample collected from each patient and completing the pathological diagnosis report during the work from the time of collection of the pathological diagnosis report. Eliminate the danger of loss or contamination of the sample, request form data, and past history data) and human error of replacement, and if such an error occurs, determine where the trouble occurred even after the fact. Be clear and clarify where you are responsible.
[0009]
In addition, in the above-mentioned work process, human work can be shortened or omitted in order to eliminate troubles that become unrecoverable and major problems due to simple human errors, and management and monitoring by automation and mechanization can be performed. This is to increase the number of work processes.
[0010]
For this reason, macro images and pathological tissue samples that can be observed and confirmed from outside the perforated dish through the window of the fixed transfer container and the window of the cassette, which have already been filed by the inventor, can be used as clinical images. The patient identification data (patient attribute, patient ID, patient chart number, etc.) written and attached at the time of collection on the side and the reception reference number on the pathology side include the collection of samples from the clinical side patients and their clinical data. Receipt of pathological diagnosis test request data and receipt / transport / reception / cut-out sorting of pathological test samples from the time of submission of pathological diagnosis request / chemical solution processing / embedding / sample preparation / history search / pathological diagnosis / data management / archiving / pathological report It is to be managed and used in a unified manner in all work processes up to the issuance of the certificate.
[0011]
For that purpose, in addition to the observation and confirmation of the macro image of the sample in the cassette, means such as a sample data number, a barcode, and a two-dimensional data code for securely linking and relating each patient data to the cassette sample were necessary. However, in the conventional embedded block manufacturing method, the data writing surface was damaged during the deburring operation of the cassette base, and mechanical automatic reading was not possible, so patient data from the clinical side could not be directly introduced into the pathological sample manufacturing process. I was in The purpose is to reliably protect the written surface of the block sample data that is also the patient sample data.
[0012]
In an embedding method using a burrs-free pathological tissue sample cassette and its embedding dish disclosed in Japanese Patent Application No. 2001-105509 already filed by the inventor, an opening is formed on the upper side and a bottom is formed on the lower side. If a dedicated bottom plate without burrs is used in combination with a cassette bottom made of a frame-shaped base on which a porous support bottom plate protruding with a smaller area than the outer peripheral surface is formed, a block block without burrs can be obtained. However, many conventional cassettes do not have a porous support bottom plate projecting with an area smaller than the outer peripheral surface of the bottom surface, and many printers for printing sample numbers (serial specification numbers and sample numbers) of cassettes conform to the shape. Made for use. Of course, it is possible to make a print printer for the cassette bottom with a protruding porous support bottom plate, or to devise the opening and the porous support bottom plate to suit the use of existing print printers. There are no burr-free embedding dishes using conventional cassette bottoms to suit use.
[0013]
For that purpose, in the conventional burr-free embedding dish and its embedding method, an opening is formed on the upper side of the cassette, and the protruding portion is formed with a smaller area than the outer peripheral surface of the lower side of the frame-shaped base which is also the adapter fitting portion. The inner peripheral surface of the opening of the burr-free embedding dish and the surface between the outer peripheral wall and the inner peripheral wall of the embedding dish opening are formed on the concave cross section of the side surface of the perforated supporting bottom plate and the outer peripheral surface of the bottom of the frame-shaped substrate wider than the perforated supporting bottom plate. The bottom of the frame-shaped base, which is formed by projecting the porous support bottom plate with an area smaller than the bottom area thereof, and the inner periphery of the embedding dish on the surface of the intersecting convex portion are fitted in a watertight manner. It is obstructive when printing with a cassette printing machine or embedding in a conventional embedding dish.
[0014]
[Means for Solving the Problems]
According to the present invention, a watertight frame (14) for integrally fitting the outer surface of a rectangular pathological sample cassette to the outer surface of an opening (2a) of an embedding dish (59) is provided. This is an embedding dish without burrs for a sample.
[0015]
A rectangular watertight frame (14) for watertight fitting with the entire outer peripheral surface (11a) of the rectangular pathological sample cassette is integrally formed outside the periphery of the opening (2a) of the embedding dish (59). It is an embedding plate without burrs for a pathological sample to be protruded.
[0016]
Furthermore, a watertight frame (14) that fits tightly with the three outer peripheral surfaces (11a) of the rectangular pathological sample cassette is integrally formed on the three sides on the outer periphery of the square opening (2a) of the embedding dish (59). A pathology which projects upward and forms a watertight upper end (4) upwardly on the other side at the periphery of the rectangular opening (2a) for watertight fitting with the outside of the porous bottom surface (10b) of the pathological sample cassette. This is an embedding dish without burrs for a sample.
[0017]
Still further, the present invention is directed to a porous support bottom plate 13 which is provided with the burr-free cassette frame-like base 11 according to the prior application of the present inventor with a smaller area than the porous bottom outside 10b below the porous bottom outside 10b. The outer peripheral surface 13a and the inner peripheral surface 7 of the embedding dish are fitted in a watertight manner, and the outer peripheral face 11a of the burr-free cassette frame base 11 and the outer peripheral face 8 of the embedding dish almost coincide with each other. It was a device for forming an embedded block of a pathological tissue sample consisting of:
[0018]
However, to print barcodes, two-dimensional data codes, and patient data such as sample numbers and patient names with the automatic printing printers of many cassettes at present, they are provided with a projecting area smaller than the outer peripheral surface of the bottom of the frame base of the cassette. The perforated indicator bottom plate becomes an obstacle.
[0019]
For that purpose, it is similar or almost similar to the cassette of the conventional shape, can be set and printed on the automatic printing printer of the existing cassette, and can be embedded in all the conventional embedding plates. Inventing an embedding dish without burrs when embedding it in a frame-shaped substrate with a dedicated embedding tool or embedding dish is very convenient, and the cost reduction and automation will be further advanced. Furthermore, if the frame base of the cassette and the embedding device are used in common, the embedding device can be integrated with a part of the cassette, so that the work process and cost can be further reduced, and automation can be further advanced. Therefore, troubles such as mistaking, mixing, and loss of patient samples are eliminated.
[0020]
For this purpose, in order to produce a burr-free embedded block cassette without a porous support bottom plate protruding with a small area from the outer perforated bottom surface 10b of the conventional burr-free cassette frame-shaped base 11, the cassette frame-shaped base and the In other words, the outer peripheral surface 11a of the frame-shaped base of the cassette frame-shaped base and the periphery of the perforated bottom surface 10b are used as a water-tight surface by reversing the uneven surface of the water-tight seal surface of the filling dish.
[0021]
To this end, the height of the watertight frame 14 of the burr-free embedding dish 59 is determined by taking the height of the watertight frame 14 of the burr-free embedding dish 59 from the outer wall of the cassette frame-like body as a means for taking out the cassette frame-like base which is an embedded block from the burr-free embedding dish. That is, it is lower than the outer peripheral surface 11a.
[0022]
Alternatively, as a means for taking out the cassette frame-shaped substrate, which is an embedded block embedded from the burr-free embedding plate, the watertight frame 14 on one side of the burr-free embedding plate 59 is removed and the cassette frame-shaped base is taken out. It is to be.
[0023]
Alternatively, as a means for taking out the cassette frame-shaped substrate which is an embedded block embedded from the burr-free embedding dish, the bottom surface of the burr-free embedding dish 59 is removed to remove a burr-free package that is not a dish provided with the watertight frame 14. The embedding frame 19 is formed and the bottom surface is used by embedding a plate at the bottom when embedding. Of course, the bottom can be easily removed so that the embedded block cassette can be pushed out from below.
[0024]
In addition, if the burr-free embedding dish 59 has no bottom and becomes the burr-free embedding frame 19, there is no bottom above and below the cassette frame-shaped base body in which there is no obstruction to the passage of the chemical solution, and there is a frame around it. Therefore, if the cassette frame-shaped base 11 and the burr-free embedding frame 19 are put together from the beginning, the three functions of the role of the basket for the reagent treatment, the role of the embedding plate, and the role as the block base are achieved. It is also possible to shorten the work steps and prevent the sample from being lost and mixed, and to make the cassette frame-shaped substrate 11 thinner.
[0025]
If the cassette frame-shaped substrate 11 is housed in the burring-free embedding frame 19 from the beginning and can be used as an integral embedding dish cassette, the frame-shaped substrate 11 can be embedded without removing the frame-shaped substrate 11. The window 22 is formed on the porous bottom surface 10 of the base 11.
[0026]
On top of that, if there is a window 22 on the porous bottom surface 10 of the cassette frame-shaped substrate 11 housed in the embedding frame 19 from the beginning, the embedding operation can be performed without attaching and detaching the cassette frame-shaped substrate. The use of these pathological tissue treatment cassette instruments together with the embedding gel fixing support of the present invention makes it possible to automate the embedding operation.
BEST MODE FOR CARRYING OUT THE INVENTION
As shown in FIGS. 1 to 4, the conventional embedding dish (FIG. 1) mounts the conventional cassette frame-shaped base 11 on the support 2 having no watertight surface of the opening 2 a of the embedding dish, and disposes the embedding agent 32. Burrs 57 are generated around the cassette frame-shaped substrate 11 of the block sample in order to produce the embedding block by pouring it into the porous bottom surface 10 of the conventional cassette. On the other hand, in the embedding dish shown in FIGS. 2 to 4, the porous support bottom plate 13 is projected downward with a smaller area than the porous bottom outer surface 10b of the other conventional cassette frame base 11, so that the porous support bottom plate 13 is provided. 13 is formed in a watertight manner by the concave diagonal surface of the outer peripheral surface 13a and the outer peripheral bottom surface 10b of the cassette frame-shaped base 11 and the convex diagonal surface of the inner peripheral surface 7 and the upper end surface 4 of the burr-free embedding dish. By fitting, no burrs 57 are generated around the cassette of the block sample.
[0027]
However, it is obvious that the same effect can be obtained even when the watertight surface is fitted in a watertight manner by the convex diagonal surface on the cassette side and the concave diagonal surface of the burr-free embedding dish. Therefore, when an embedding dish 59 having a shape as shown in FIGS. 5 to 9 is formed, the embedding dish 59 has a smaller area than the outer bottom surface 10b of the cassette frame-shaped substrate 11 of FIGS. Even without the protruding perforated support bottom plate 13, the same effect can be obtained with the conventional cassette in the shape of the watertight fitting portion of the burr-free embedding dish, although the shape of the watertight fitting portion is reversed. The shape of the cassette frame-shaped substrate 11 can be variously manufactured. As shown in FIGS. 3 to 10, not only a conventional trapezoid or box but also an inverted trapezoid (right in FIG. 3) can be embedded without burrs. However, in the case of this embedding dish, the take-out portions 16 to 16a of the frame base 11 of the cassette are required to easily take out the embedding block from the burr-free embedding dish.
[0028]
Therefore, as shown in FIGS. 10 to 13, the housing bottom 3 of the burr-free embedding dish 59 shown in FIGS. 5 to 6 and 9 is removed, and the upper opening 2a and the lower embedding frame open bottom 18 are also closed. A cylindrical burr-free embedding frame 19 having no surface and having a lower embedding frame opening bottom portion 18 opened from the upper and lower opening portions 2a is formed, and the lower embedding frame opening of the cassette frame base 11 is performed. A package formed so as to be narrower than the bottom 18 side and slightly smaller than the entire bottom surface of the frame-shaped substrate surrounded by the cassette frame-shaped substrate outer peripheral surface 11a, so that the periphery of the cassette frame-shaped substrate does not fall down. It has an upper end surface 4 of the filling tray, and is formed into a size that is equal or narrower from the embedding storage portion 20 below to the embedding frame open bottom surface 18 and the left and right ends of the final lower embedding frame opening bottom portion 18. Alternatively, a reference mark that is vertically asymmetric can be formed and A large embedding dish or general-purpose embedding dish 54 or a single thin plate (bottom plate for burial-free embedding frame) 29 is laid and sealed, and the embedding agent 32 is injected into the cold plate. When the embedding agent such as paraffin is transferred and solidified and solidified, a burr-free embedded block sample can be prepared.
[0029]
Further, when an embedding block sample is prepared using the burring-free embedding frame 19 of this shape, the cassette frame-shaped substrate 11 can be made water-tight on all four sides so that burrs are not completely attached. In contrast, since the bottom is open without a bottom portion, the block cassette sample without burrs can be easily taken out by pushing the bottom portion upward. FIG. 14 is a plan view at the time of embedding, and a flowing embedding agent 32 is poured from the porous bottom surface 10 of the cassette frame-shaped substrate 11.
[0030]
Further, as shown in FIGS. 15 to 18, a window 22 is formed in the porous bottom portion 10 of the cassette frame-shaped base, and a small chamber for separately storing small samples such as a biopsy sample around the window 22. When the frame 23 is separated and formed by the partition wall 24, the cassette frame-shaped base 11 is fitted into the burial-free embedment frame 19 while the cassette frame-shaped substrate 11 is formed on the lower surface of the embedment frame 19. Alternatively, a biopsy sample or the like can be arranged and fixed on the bottom surface of the slice inspection on the bottom plate 29 for a burr-free embedded frame, and a burr-free embedded block cassette sample can be manufactured.
[0031]
Further, as shown in FIGS. 19 to 31, the cassette frame-shaped substrate 11 is previously mounted in an embedding frame without burrs, and a sample tissue is stored in the frame-shaped substrate, and the cassette frame-shaped substrate 11 is mounted together. When the lid 26 is closed to the opening 2a of the burr-free embedding frame 19, the sample in the cassette frame-shaped substrate together with the embedding frame 19 is set in an automatic fixed embedding device, and a chemical treatment of dehydration, degreasing and penetration is performed. be able to. At this time, it is convenient to use a cassette bottom in which the window 22 is not formed on the porous bottom surface 10 of the frame-shaped base 11. It is not always the case that the surgical excision material has a fixed tissue shape, but there is also an excision material (fragment sample) that scrapes the tissue of the diseased part. There is a high risk of spilling out of the sample, and it may be necessary to process larger amounts of tissue than the biopsy sample. For such a case, as shown in FIG. 19 and FIG. 20, the cassette frame-shaped substrate 11 of the porous bottom surface 10 having a small through-hole of the porous bottom surface 10 without the window 22 can be formed.
[0032]
Furthermore, as shown in FIGS. 32 to 42, when the inventor applies the shape of the cassette frame-shaped substrate having a patent registration already, the thickness of the embedded block cassette sample becomes thinner. can do. When the embedded block cassette sample is sliced, as shown in FIGS. 37 and 42, a U-shaped microtome adapter thin cassette thickness adjusting plate 33a is set on the protruding edge of the cassette frame-like base, and a conventional cassette is formed. The size of the external method 40 × 28 × 6 mm is secured.
[0033]
Further, as shown in FIGS. 38 to 41, the shape of the ridge 40 and the lower edge 41 of the projecting container of the cassette frame-shaped base 11 are formed into the same square size. When a cassette lid and a burr-free embedding frame 19b having a cassette lid fitted in a manner that covers the outer method in a watertight manner and a bottom plate insertion port 42 for a burr-free embedding frame near the inner side of the porous liquid surface 60 are formed, Without discarding the cassette lid / burr-free embedding frame 19b, which is the lid after the chemical solution treatment, and inserting the bottom plate 43 for the burr-free embedding frame for insertion from the bottom plate insertion port 42 for burr-free embedding frame, When the embedding frame 19b without lid and burr is fitted to the lower edge portion 41 of the projecting container and used as an embedding dish, an embedding block cassette sample without burr can be obtained. At this time, the operation of inserting and removing the bottom plate 43 for the burial-free embedding frame for insertion is performed by holding the take-out handle portion 45. A reference mark 5 is formed at a corner inside the inside of the cassette lid / burr-free embedment frame 19b. Is formed, the embedding block embedded by this embedding method can produce an embedding block with the reference mark 5 without burrs. Of course, the thin cassette thickness adjusting plate 33a for the U-shaped microtome adapter can also be used when the embedded block cassette is sliced.
[0034]
Still further, the present invention prevents the burrs from being generated and adhered by water-tightly fitting the convex diagonal surface of the cassette frame-shaped substrate of all shapes and the concave diagonal surface of the burring-free embedding dish. Burr-free embedding can be performed at any position if the substrate has a diagonal surface. Therefore, the thickness of the cassette frame-like substrate can be changed, and the thickness of the cassette frame-like substrate 11 and the burr-free embedding frame 19 shown in FIGS. Is formed into a thickness equivalent to the thickness of the conventional frame base of the cassette, and the embedding plate cassette (FIG. 49) To FIG. 53). At this time, as shown in FIG. 12, the inner surface 14a of the burr-free embedding dish 19 of the burr-free embedding frame 19 is formed on the outer surface of the cassette frame-shaped base 11 so as not to be in the way when the microtome adapter is fitted. When the same taper 14c is attached, the completed burr-free embedded block cassette is removed and it is cheap to take out. The embedding frame retaining feathers 26d of the embedding dish cassette lid in FIGS. 23 to 28 are provided for holding the embedding frame in the horizontal direction.
[0035]
Furthermore, as shown in FIG. 43 to FIG. 48 and FIG. 52 to FIG. 53, the immobilized transfer (biopsy sample) is further performed by using the embedding gel fixing support 46 already applied for invention by the applicant. When the plate 55 is used, the embedding operation can be performed without removing the cassette frame-shaped substrate 11 through the window 22 formed in the cassette frame-shaped substrate 11 in which the biopsy sample is also contained in the burrs-free embedding frame 19. Can be completed. This means that the embedding operation can be automated by using the embedding gel fixing support 46, the (biopsy sample) fixing transfer plate 55, and the burr-free embedding dish cassette.
[0036]
Further, FIGS. 43 to 46 show that the biopsy samples 31 are arranged in the biopsy sample V-shaped valley storage location 47 in the order of the position number 52 and the embedded gel fixing support 46 is poured into the biopsy sample 31 and the sample backing plate 49 is placed there. When the biopsy sample 31 is fixedly held, the biopsy sample 31 fits across the V-shaped valley regardless of the diameter of the biopsy sample 31, and the centers of the biopsy samples 31 are aligned on the same straight line. Line up. As a result, the biopsy sample 31 united with the embedded gel-fixing support 46 arranged in the biopsy sample V-shaped valley storage location 47 has the sample-embedded thin-section surface 50 in the embedding dish storage bottom. When embedded in the embedding frame-like bottom plate 29 or the burrs-free embedding frame-like bottom plate 29, the biopsy samples 31 in all the embedding gel-fixed supports will be cut together and sliced together with a maximum area. The sample backing plate 49 of the fixed transfer plate 55 shown in FIGS. 43 and 44 is preferably a cardboard or a porous plate made of, for example, polyvinyl alcohol. In particular, if a polyvinyl alcohol sample backing plate is used, patient data can be described, and it can be used for simple fixation and backing on the clinical side (sample backing portion 56), and can be sliced, as shown in FIGS. 45 and 46. Can be embedded as is. 47 and 48 show another embodiment of the fixed transfer plate.
[0037]
Although the present invention has been described with respect to the above-described embodiment, the present invention is not limited to the embodiment, and specific technical means for more effectively implementing the embodiment may be added, or the embodiment may be modified. It can be partially changed.
[0038]
For example, when the above-mentioned burring-free embedding dish cassette is used, mixing and replacement of the sample are completely eliminated, and simultaneous embedding becomes possible with a plurality of general-purpose embedding dishes 54a as shown in FIG. Work efficiency is further improved.
[0039]
【The invention's effect】
As described above, the present invention provides a convex diagonal surface or one surface where the outer peripheral surface of the cassette frame-shaped base intersects with the bottom and a concave diagonal surface or one surface where the watertight frame of the burr-free embedding dish intersects with the upper end surface of the embedding dish. The cassette frame-like substrate is fitted into the watertight frame on the top surface of the embedding dish in a watertight manner so that the embedding agent on the bottom of the embedding dish does not overflow onto the outer peripheral surface of the cassette frame-like substrate. In the case of the burrs-free embedment frame, which is a development of the embedment operation, even if the height of the watertight frame around the entire circumference is the same as the height of the outer peripheral surface of the cassette frame-shaped substrate, that is, the thickness of the frame-shaped substrate. The completed embedding block cassette can be easily taken out by pushing the embedding frame open bottom of the frame-shaped base upward, so that a cassette take-out part such as a burr-free embedding dish is not required. Therefore, between the porous bottom surface of the cassette frame-shaped substrate and the open bottom lid of the burrs-free embedment frame, and when a window is formed on the porous bottom surface of the cassette, the lid of the embedment dish cassette and the burrs-free embedment frame open bottom side lid A space completely surrounded by a porous surface is created between the two, and when this space is used as a storage part for a sample, a cassette in which the embedding function is incorporated as an embedding dish cassette is completed.
[0040]
Using the burr-free function of the burr-free embedding dish and the burr-free embedding frame makes it possible to mold the thickness of the cassette frame-shaped substrate to be thinner. Depending on the size of the sample, the small sample is stored inside the thin cassette frame base, and the large sample is stored in the sample storage section of the burial-free embedding frame, from the small sample of the above to the large sample that is surgically extracted and trimmed. It can be stored and used for chemical solution treatment of an automatic fixed embedding device with fixed dehydration, degreasing and transparent penetration.
[0041]
Further, in the embedding dish cassette of the present invention, since the cassette frame-shaped substrate is mounted on the embedding frame from the beginning, there is no need to select or mount the embedding dish at the time of embedding. Set the embedding dish cassette on the embedding dish), open the lid, and place a thin section of the test sample on the bottom plate (or general-purpose embedding dish) through the window at the bottom of the perforated plate to remove the fluid embedding medium. It only needs to be poured and cooled.
[0042]
Also, since the cassette frame base can be made thin, it is set on a microtome adapter by laying it on a thickness adjusting plate when slicing, and the block cassette sample is thinly stored when storing block samples because it is thinner than the conventional cassette frame base. Requires less space.
[0043]
Further, since the shape of the cassette frame-shaped substrate can be formed according to the conventional shape, it can be used for a conventional cassette printing printer.
[0044]
In addition, when used together with an embedding gel fixing support and a fixed transfer plate, multiple biopsy samples can be embedded as one sample without touching any sample, and the embedding of small samples can be automated. became. Of course, if the patient's name is described on the fixed transfer plate, later confirmation can be assured.
[Brief description of the drawings]
FIG. 1A is a plan view of a conventional embedding dish. FIG. 1B is a cross-sectional view taken along the line IB-IB of FIG. 1A.
FIG. 2 is a plan view of a burr-free embedding dish according to the prior application of the present applicant.
3A is a cross-sectional view taken along the line IIIA-IIIA of FIG. 2 and a cross-sectional view of a frame-shaped base of the burr-free cassette. FIG. 3B is a cross-sectional view of a portion corresponding to the line IIIA-IIIA of FIG. 2 of the burr-free cassette frame base and the burr-free embedding dish having shapes different from those of FIG. 2A.
FIG. 4 is a cross-sectional view of a burr-free embedding dish, a burr-free cassette, and a base.
FIG. 5A is a perspective view of an embodiment of the embedding dish of the present invention. FIG. 5B is a perspective view of another embodiment of FIG. 5A.
FIG. 6 is a plan view of FIG. 5A.
FIG. 7A is a cross-sectional view of an embodiment of a cassette frame-like base fitted into the embedding dish of FIGS. FIG. 7B is a sectional view of an embodiment different from FIG. 7A. (C) is a side view of the embodiment of the embedding dish watertight frame and the burr-free embedding dish. FIG. 7D is a side view of the embodiment different from FIG. 7C. FIG. 7E is a side view of the embodiment different from FIG. 7D. FIG. 7F is a side view of the embodiment different from FIG. 7E.
FIG. 8A is a longitudinal sectional view of the cassette frame-shaped base of FIG. 7A. FIG. 8B is a longitudinal sectional view of the embodiment in which the cassette frame-shaped substrate of FIG. 8A is fitted into an embedding dish. (C) is sectional drawing of a part of embedding dish of FIG. 8B. (D) is a longitudinal sectional view of FIG. 7B. (E) is a longitudinal cross-sectional view of the embodiment in which the part of FIG. 8D is fitted to the embedding dish. FIG. 8F is a longitudinal sectional view of a part of FIG. 8E and an enlarged view of the part.
FIG. 9A is a side view of another embodiment of FIG. 7A. (B) is a side view of the embedding dish in which the cassette of FIG. 9A is fitted. FIG. 9C is a side view of an embodiment different from FIG. 9A. (D) is a side view of the embedding dish which fits the cassette of FIG. 9C. (E) is a plan view of FIG. 9D.
FIG. 10 is a perspective view of an embedding dish of an embodiment different from FIGS. 5A and 5B.
FIG. 11 is a longitudinal sectional view showing an embodiment of an embedding frame without burrs of the present invention.
FIG. 12 is a sectional view taken along line XII-XII of FIG. 11;
FIG. 13A is a cross-sectional view of a cassette frame-shaped base according to another embodiment. (B) is an embedding frame without burrs fitted with the portion of FIG. 13A. (C) is a longitudinal cross-sectional view of a general-purpose embedding dish for mounting the portion of FIG. 13B.
FIG. 14 is a plan view when the parts shown in FIGS. 13A, 13B, and 13C are fitted.
FIG. 15 is a plan view of a cassette frame-shaped substrate showing another embodiment.
16 is a vertical sectional view taken along the line XVI-XVI of FIG.
FIG. 17 is a longitudinal sectional view of the embodiment of the example showing a state in which the embedding dish cassette lid is closed by being fitted into the burr-free embedding frame of the portion of FIG. 16;
FIG. 18 is a sectional view taken along line XVII-XVII in FIG. 17;
FIG. 19 is a longitudinal sectional view of another embodiment different from FIG. 17;
20 is a longitudinal sectional view of an embodiment of an example showing a state of an embedding frame without burrs fitted with another box-shaped cassette frame base in the embodiment of FIG. 19;
FIG. 21 is a longitudinal sectional view showing the relationship between the frame-shaped substrate, the burrs-free embedment frame, and the bottom plate for burrs-free embedment frame at the time of embedding when the box-shaped cassette frame-shaped substrate of FIG. 20 is used.
FIG. 22 is an example of an embodiment showing a state in which a window is provided on a porous bottom surface of a generally-used conventional cassette frame-shaped base, fitted to a burr-free embedding frame, and an embedding dish cassette lid is closed. It is a longitudinal cross-sectional view of a form.
FIG. 23 is a transverse sectional view taken along the line XXIII-XXIII of FIG. 22;
FIG. 24 is a side view of each part of the embodiment in FIG. 22;
FIG. 25 is a plan view of each part of the example of FIG. 22;
26 is an example of a back view of the embedding dish cassette lid, which is one part of FIG. 25.
FIG. 27 is a side view of the form in which the parts of FIG. 24 are fitted and set.
FIG. 28 is a plan view of FIG. 27;
FIG. 29 is a plan view of an example of a triple general-purpose embedding dish that can be used when embedding a burr-free embedding frame.
FIG. 30A is a cross-sectional view showing the relationship and shape between a thin cassette thickness adjusting plate for a microtome adapter used when slicing a thin cassette frame base and the frame base. FIG. 31B is a cross-sectional view showing another example of a part of FIG. 31A.
FIG. 31 (A) is a side view showing a relationship between a microtome adapter and an embedding block cassette. (B) is sectional drawing of the Example different from FIG. 31A.
FIG. 32 (A) is a perspective view showing the shape of a thin cassette frame-shaped base of the applicant's earlier patent. FIG. 32B is a cross-sectional view of another embodiment different from FIG. 32A. FIG. 32 (C) is a sectional view of another example different from FIG. 32B.
FIG. 33 is a perspective view showing the relationship between the cassette frame-shaped substrate and the burial-free embedding frame of FIG. 32;
34 (A) is a longitudinal sectional view showing the relationship between the cassette frame-like substrate of FIG. 33 and a burr-free embedding frame or a burr-free embedding dish. 34B is a cross-sectional view of another embodiment of a portion of FIG. 34A.
FIG. 35 is a longitudinal sectional view showing the embedding operation of FIG. 34A.
FIG. 36 is a longitudinal sectional view showing a relationship between a burr-free embedded block cassette manufactured by the embedding operation of FIG. 35 and a thin cassette thickness adjusting plate thereof.
FIG. 37 is a perspective view of each part in FIG. 36;
FIG. 38 is a perspective view of another embodiment of FIG. 34.
FIG. 39 is a perspective view illustrating the parts of FIG. 38;
FIG. 40 is a longitudinal sectional view of a cassette as the sample basket of FIG. 38;
FIG. 41 is a longitudinal sectional view for explaining use when embedding in FIG. 40.
FIG. 42 is a side view showing the relationship between the thin cassette thickness adjusting plate of FIG. 36 and the cassette microtome adapter.
FIG. 43 (A) is a plan view showing an example of a fixed transfer plate. FIG. 43B is a cross-sectional view taken along the line XXXXIII-XXXXIII of FIG. 43A.
FIG. 44 is a cross-sectional view showing the relationship between the biopsy sample, the sample backing plate, and the embedded gel-fixing support in the enlarged view of the cross-sectional view of FIG. 43B.
FIG. 45 is a diagram showing the usage and positional relationship between a biopsy sample, a sample backing plate, and an embedded gel-fixing support.
FIG. 46 is a longitudinal sectional view of the embedding operation of FIGS. 44 and 45.
FIG. 47 is a plan view of another embodiment of FIG. 43A.
FIG. 48 is a perspective view of another embodiment of FIG. 47;
FIG. 49 is a diagram showing a comparison of the thickness between the burr-free embedding dish cassette of the present invention and a conventional cassette.
FIG. 50 is a longitudinal sectional view of each part of the other embodiment of FIG. 22;
FIG. 51 is a cross-sectional view showing a comparison of the thickness of a representative example of an embedding tray cassette without burrs.
FIG. 52 is a cross-sectional view showing a comparison of the thickness at the time of embedding of the embedding tray cassette without burrs.
FIG. 53 is a cross-sectional view showing a comparison of the thickness between the slicing and storage of the burring-free embedding dish cassette.
[Explanation of symbols]
1 Embedded substrate holder frame-shaped substrate
2 Holder
3 accommodation bottom
4 Embedding dish top surface
5 Reference mark
6 Embedding dish tabs
7 Inner peripheral surface of embedding dish
8 Outer peripheral surface of embedding dish
9 (Sample) Data description surface
10. Cassette porous bottom
10a Porous bottom formed in the center of frame-shaped substrate
10b Cassette porous bottom outside
11 Cassette frame base
11a Outer peripheral surface of cassette frame base
11b Lid locking groove of cassette frame base
11c Intersection between outer peripheral surface of cassette and outer surface of porous bottom
12 Position where the cassette adapter is hooked
13 Peripheral surface of perforated support bottom plate
14 Embedding dish watertight frame
14a Inner peripheral surface of embedding dish watertight frame
14b Outer surface of embedding dish watertight frame
14c tapered embedding dish watertight frame
14d Burrs-free embedded watertight frame side locking projection
14e Watertight frame tip
14f Shape according to cassette side wall surface shape
14g Watertight frame lower than the outer surface of the cassette frame base outer wall
15 Cassette frame-shaped substrate outer surface
16 Cassette take-out section
16a Cassette removal recess
17 Box-shaped cassette frame-shaped substrate side surface
18 Embedding frame open bottom
19 Burr-free embedding frame
19a Burr-free embedded frame open bottom side locking projection
19b Cassette lid / burr-free embedding frame
19c perforated plate
19d Embedding frame inner wall
20 Embedding storage section
21 Cassette opening
22 window
23 Biopsy storage room
24 Small room partition and reinforced wall
25 Window frame and partition reinforcement wall
26 Embedding dish cassette lid
26a Embedding dish cassette lid locking piece
26b Locking protruding piece of embedding dish cassette lid
26c Embedding dish cassette lid tab
26d Embedding tray cap feather for embedding tray cassette lid
27 Burr-free Embedding Frame Open Bottom Side Lid
28 Sample storage section
29 Bottom plate for embedded frame without burrs
30 Embedding dish communication outlet
31 Biopsy (small pathological tissue) sample
32 embedding agent
33 Thin cassette thickness adjustment plate for microtome adapter
33a Thin cassette thickness adjustment plate for U-shaped microtome adapter
34 air vent
35 Insert cassette protrusion
36 Microtome sword
37 Normal cassette thickness
37a Thin cassette thickness
38 Cassette Microtome Adapter
39 Microtome block fixing part
40 ridge
41 Lower edge of projecting container
42 Bottom slot for burr-free embedded frame
43 Bottom plate for embedding frame without burrs for insertion
44 Notch for fiducial mark
45 Removal handle
46 Embedded gel fixing support
47 V-shaped valley storage location for biopsy sample
48 Sample backing plate holding projection
49 Sample backing plate
50 Sample embedded thin section
51 tweezers
52 Sample position number
53 Patient Name Data Location
54 General purpose embedding dish
54a Triple general-purpose embedding dish
55 fixed transfer plate
55a Perforated bottom of fixed transfer plate
56 Sample backing
57 Bali
58 Cassette Lid
58a Porous liquid inlet
59 Burr-free embedded dish
60 perforated liquid surface

Claims (18)

A watertight frame (14) for watertight fitting with an outer surface of a square pathological sample cassette is integrally provided outside the periphery of the opening (2a) of the embedding dish (59). Burr-free embedding dish for pathological samples A rectangular watertight frame (14) for watertight fitting with the entire outer peripheral surface (11a) of the rectangular pathological sample cassette is integrally protruded from the periphery of the opening (2a) of the embedding dish (59). Burr-free embedding dish for pathological samples 3. The pathological sample embedding dish according to claim 1, wherein the height (h) of the watertight frame (14) is smaller than the height (H) of the outer peripheral surface of the pathological sample cassette. A watertight frame (14) that fits tightly with the three outer peripheral surfaces (11a) of the rectangular pathological sample cassette is integrally protruded on three sides on the outer periphery of the rectangular opening (2a) of the embedding dish (59). And a water-tight upper end surface (4) for water-tightly fitting with the outside (10b) of the porous bottom surface of the pathological sample cassette on one side remaining at the periphery of the rectangular opening (2a). Burr-free embedding dish for pathological samples characterized by the following: A watertight frame (14), which is fitted in a watertight manner with the outer surface of the rectangular pathological sample cassette, is integrally protruded from the periphery of the opening (2a) of the embedding dish (59). A burial-free embedding frame (19), and the open bottom (18) of the embedding frame (19) is placed on the upper surface of the bottom plate (29) in a watertight manner. Burial dish A watertight frame (14) that fits in a watertight manner with the outer surface of the rectangular pathological sample cassette is integrally protruded outside the periphery of the opening 2a of the embedding dish 59). A pathological sample comprising a burial-free embedding frame (19) around the periphery, and an open bottom portion (18) of the embedding frame (19) placed on the upper surface of a general-purpose embedding dish (54) in a watertight manner. Embedding dish 3. An upper edge (4) engaging with a lower edge of an outer surface of a square sample cassette is formed at a periphery of an opening (2a) of an embedding dish (59). Burr-free embedding dish for pathological samples A cassette frame base (11) having an opening (21) formed on the upper side and a porous bottom surface (10) formed below, and a cassette opening (21) of the cassette frame base 11) which is detachably attached. In an embedding dish fitted with a pathological sample cassette comprising a lid (58) having a fitted porous liquid inlet (58a), an outer peripheral surface of an outer wall of the cassette frame base (11) is provided. 11a) is fitted to the inner peripheral surface (14a) of the watertight frame of the embedding dish (59) of the pathological sample in a watertight manner, and the outer periphery of the cassette is a hook portion of an adapter for detaching the cassette of the cassette to the microtome with one touch. The cassette is hooked at the intersection (11c) of the surface (11a) and the outer surface of the porous bottom (10b), and the height of the outer peripheral surface (11a) of the outer wall of the frame-shaped substrate (11) increases the height of the embedding dish. (59) of the inner peripheral surface (14 g) of the watertight frame Although the thickness is low, the thickness of the watertight frame (14) at the opening (2a) of the embedding dish (59) is such that the embedding agent (32) is in contact with the outer peripheral surface (11a) of the outer wall of the frame-shaped substrate (11). The embedding dish (59) has a thickness (about 0.5 mm) that does not accumulate much at the contact point (14e) of the watertight frame (14g), or the opening ( An embedding dish for forming an embedding block for a pathological sample, characterized by having an embedding dish opening sloped from inside to outside at a point (14e) of the watertight frame (14) of 2a). A frame-shaped substrate 11 having an opening 21 formed on the upper side and a bottom surface 10 having a porous liquid passage port on the lower side thereof; and a porous member 11 detachably fitted to the opening 21 of the frame-shaped substrate 11. In the cassette for a pathological sample composed of the lid 58 having the liquid inlet 58a, the outer peripheral surface 11a of the three outer walls of the outer surface 10b of the rectangular porous bottom surface of the frame-shaped substrate 11 is embedded in a pathological sample embedding dish 59 (see FIG. 5B, 6, 7B, 8, 9) The outer peripheral surface 11a of the cassette, which is a hook portion of an adapter which is fitted to the inner peripheral surface 14a of the three-way watertight frame in a watertight manner and detaches the cassette of the cassette from the microtome with one touch. And the crossing portion 11c of the perforated bottom surface 10b, the cassette is hooked, and the height H of the outer peripheral surface 11a of the three outer walls of the cassette frame base 11 and the embedding dish 59 (FIGS. 7, 8, 9 left) The height of the inner peripheral surface 14a of the three-way watertight frame h is substantially the same as that of the cassette frame-shaped base 11 and the one-sided watertight frame 14 of the embedding dish 59 is characterized by having an opening 2a of the embedding dish that almost coincides with h. An embedding dish for forming an embedding block of a pathological sample, having the morphological features and functions described. A frame-shaped substrate 11 having an opening 21 formed on the upper side and a bottom surface 10 having a porous liquid passage port on the lower side thereof; and a porous member 11 detachably fitted to the opening 21 of the frame-shaped substrate 11. In the cassette for a pathological sample composed of the lid 58 having the liquid inlet 58a, the outer peripheral surface 11a of the three outer walls of the outer surface 10b of the rectangular porous bottom surface of the frame-shaped substrate 11 is embedded in a pathological sample embedding dish 59 (see FIG. (5, 6, 7, 8, 9) The outer peripheral surface 11a of the cassette, which is a hook portion of an adapter which is fitted in a watertight manner with the inner peripheral surface 14a of the three-way watertight frame and detachably attaches and detaches the cassette of the cassette to the microtome with one touch. And the crossing portion 11c of the outer perforated bottom surface 10b, the cassette is hooked, and the height of the outer peripheral surface 11a of the three-side outer wall of the frame-like base 11 and the height of the embedding dish 59 (left of FIGS. ) Almost coincides with the height of the inner peripheral surface 14a of the three-way watertight frame. The embedding dish (FIG. 8) has no watertight frame 14 on the other side, and the lower bottom surface of the cassette frame base 11 is placed on the embedding dish. An embedding dish for forming an embedding block for a pathological sample, characterized in that it has a morphological feature and a function of being formed in a shape covered and closed by the end face 4. A frame-shaped substrate 11 having an opening 21 formed on the upper side and a bottom surface 10 having a porous liquid passage port on the lower side thereof; and a porous member 11 detachably fitted to the opening 21 of the frame-shaped substrate 11. In the cassette for a pathological sample composed of the lid 58 having the liquid passage opening 58a, the outer peripheral surface 11a of the outer wall of the frame-shaped substrate 11 is attached to the embedding frame 19 (FIGS. 10, 11, 12, and 13) of the pathological sample. The inner peripheral surface 14a of the watertight frame has an embedding dish opening 2a that fits in a watertight manner, and the outer peripheral surface 11a of the cassette and the outer surface of the porous bottom surface, which are a hook portion of an adapter for detachably attaching the cassette of the cassette to the microtome with one touch. 10b, the height of the outer peripheral surface 11a of the outer wall of the frame-shaped base 11 and the height of the inner wall of the embedding frame 19 (FIGS. 10, 11, 12, and 13). Are almost the same, and the embedding frame 19 Burrs without packaging Umawaku to and forming a embedded block pathological samples the inner space covered with embedded frame for the bottom plate 19 or universal embedding boat 54 to Hosoko section 18 as embedding dish. A frame-shaped substrate 11 having an opening 21 formed on the upper side and a bottom surface 10 having a porous liquid passage port on the lower side thereof; and a porous member 11 detachably fitted to the opening 21 of the frame-shaped substrate 11. In the cassette for a pathological sample composed of the lid 58 having the liquid passage opening 58a, the outer peripheral surface 11a of the outer wall of the frame-shaped substrate 11 is attached to the embedding frame 19 (FIGS. 10, 11, 12, and 13) of the pathological sample. An outer peripheral surface 11a and a perforated bottom surface 10b of the cassette, which has an embedding opening 2a which fits in a watertight manner with the inner peripheral surface 14a of the watertight frame, and is a hook portion of an adapter for attaching and detaching the cassette of the cassette to the microtome with one touch. And the height of the inner peripheral surface 14g of the watertight frame of the embedding frame 19 is lower than the height of the outer peripheral surface 11a of the outer wall of the frame-shaped base 11, but the embedding is performed. Watertight frame 14 at opening 2a of plate frame 19 The thickness is set to a thickness (about 0.5 mm) such that a large amount of the embedding agent 32 does not accumulate on the outer peripheral surface 11a of the outer wall of the frame-shaped substrate 11 and the contact point 14e of the watertight frame 14g of the embedding frame 19. Alternatively, the embedding frame 19 has an embedding dish opening 2a inclined from the inside to the outside at the pointed end 14e of the watertight frame 14 in the opening 2a of the embedding frame 19, An embedding frame without burrs, wherein an embedding block for a pathological sample is formed by laying an embedding bottom plate 19 or a general-purpose embedding dish 54 and using an inner space thereof as an embedding dish. In the embedding frame 19, the embedding and accommodating portion 20, which is a space between the pathological tissue sample and the embedding agent filling portion surrounded by the embedding frame inner wall 19d and the open bottom portion 18, is asymmetrical left and right or up and down. The embedding frame inner wall 19 is formed, and the thin section of the embedding block of the sample embedded with the embedding agent is sliced asymmetrically left and right or up and down, and the direction of sticking / extending on the slide and the direction in the thin section are described. 13. The flash-free embedding frame according to claim 11, wherein a reference mark for clarifying an arrangement order of the samples is formed on the embedding block. In the cassette frame-like base 11 which is fitted in the embedding dish 59 and the opening 2a of the embedding frame 19 in a watertight manner, the four or three side walls of the outer peripheral surface 11a of the cassette are opposed to the outer surface 10b of the cassette porous bottom. The taper according to any one of claims 8, 9, 10, 11, and 11, wherein the taper has a taper that is slightly obtuse from a right angle so as not to hinder installation of the cassette in a conventional adapter that is detachably attached to the microtome. 14. The cassette according to 12, 13. 9. The window frame 22 is formed in the porous bottom surface 10 of the cassette frame-like base 11 which fits in the embedding dish 59 and the opening 2a of the embedding frame 19 in a watertight manner. , 9, 10, 11, 12, and 13, the flashless embedding method using the embedding dish and the embedding frame. In the cassette frame-shaped substrate 11 which fits in the embedding dish 59 and the opening 2a of the embedding frame 19 in a watertight manner, the porous bottom surface 10 is formed at an intermediate portion of the frame-shaped substrate 11. A flash-free embedding method using the cassette, the embedding dish and the embedding frame according to claim 8, 9, 10, 11, 12, 131, 2, 3, 4, 5, or 6. In the cassette frame-shaped substrate 11 which fits in the embedding dish 59 and the opening 2a of the embedding frame 19 in a water-tight manner, the thickness of the frame-shaped substrate 11 is made smaller than the thickness of the conventional cassette frame-shaped substrate. 14. The cassette according to claim 8, wherein the embedding dish and the cassette are integrally formed, and the burr-free packaging using the embedding dish and the embedding frame. Filling method. A window 22 is formed in the porous bottom surface 10 of the cassette frame-like base 11 which fits in the embedding dish 59 and the opening 2a of the embedding frame 19 in a watertight manner. In the cassette, the embedding dish, the embedding frame and the embedding method without burrs described in 11, 12, and 13, a V-shape for storing a biopsy sample in a part or inside or outside of the cassette is provided. A biopsy sample storage method in which a storage portion 47 is formed, a biopsy sample is arranged there, and the inspection thin-section surface 50 is aligned in a straight line.

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