JP3877832B2 - Histopathology cassette - Google Patents

Description

[0001]
[Industrial application fields]
The present invention relates to a histopathological examination cassette. More specifically, information for identifying a specimen can be entered for each specimen storage section, and a cassette for pathological examination in which the entry section can be visually recognized even when the lid is closed, and a plurality of cassettes in close contact with each other when immersed in a drug solution. The present invention relates to a cassette for examination of pathological tissue in which a chemical solution can easily enter the inside.
[0002]
[Prior art]
In order to prepare a specimen for examining a pathological tissue, a histopathological examination cassette (hereinafter referred to as a cassette) is used. The process of making a biopsy specimen, for example, using a cassette is as follows.
(1) Place the specimen in a cassette and immerse it in a fixative such as formalin to infiltrate the fixative into the tissue to prevent tissue decay.
(2) Remove the fixative of (1) with tap water.
{Circle around (3)} Remove water contained in tissue cells by soaking in several alcohols with different concentrations.
{Circle around (4)} Since this alcohol and the paraffin to be finally permeated are not mixed, xylene, chloroform or the like that can be mixed in both of them is permeated as a mediator.
(5) Immerse in molten paraffin to infiltrate the paraffin into the tissue.
(6) Remove the chemical-treated sample from the cassette and make a paraffin block.
(7) A paraffin block is sliced together with a specimen and affixed to a slide glass, and a medical condition is examined with a microscope or the like.
[0003]
An example of a conventional cassette is shown in FIG. What is shown in the figure is a cassette described in JP-A-8-285745. The cassette 4 includes a cassette body 41. The cassette body 41 is provided with a required number of sample storage portions 42 formed by dividing the inside thereof, and has a large number of liquid passage holes at the bottom thereof. In addition, information entry sections 43 and 44 are provided at the front and side of the cassette body 41. The upper opening 45 of the cassette body 41 is provided with a lid 46 having a large number of liquid passage holes so as to be opened and closed.
[0004]
[Problems to be solved by the invention]
The cassette described in the above publication is sufficiently useful for preparing a specimen of a pathological tissue, but has the following problems.
(A) Especially in the case of biopsy, for example, even when collecting from one organ, a plurality of living tissues are collected at different locations and examined. In this case, different samples are accommodated in the respective sample accommodating portions of the cassette body, but it is necessary to be able to understand the contents of the samples for each sample accommodating portion so as not to make a mistake.
An information entry unit is provided at the front and side of a conventional cassette body, but the front information entry unit is provided not corresponding to any sample accommodation unit. It is difficult to use if you have to fill in information.
In addition, since the information entry section provided on the side of the cassette body has a vertical entry surface, there is a concern that the specimen may be spilled when the lid is opened and the specimen is accommodated, making entry difficult. Furthermore, after entry, the entry surface is not visible from the upper surface side, so that it is difficult to visually recognize.
[0005]
(B) As described above, in the step of preparing the specimen, the operation of immersing the cassette containing the specimen in the chemical solution is performed several times. In this operation, a large number of cassettes may be immersed together. In this case, the cassettes are stacked or closely adhered in the lateral direction and accommodated in the liquid tank.
Since the conventional cassette has both a flat top surface and a bottom surface of the main body, when the cassettes are stacked, for example, the stacked portions are in close contact with each other, and a chemical solution cannot enter.
Therefore, since the chemical solution that has passed through the other cassette enters the cassette that is sandwiched between the other cassettes, the chemical solution cannot enter the cassette quickly, so that the chemical processing capacity decreases, and the work It tends to cause a decrease in efficiency.
[0006]
The present invention solves the above-described problem, and it is easy to enter information for identifying a sample for each sample storage portion of the storage portion in a cassette, and the histopathological examination allows the information to be visually recognized from the upper surface side even in a closed state. The purpose is to provide a cassette.
It is another object of the present invention to provide a histopathological examination cassette in which a chemical solution can easily enter the inside even when a plurality of cassettes are closely attached vertically and horizontally when immersed in the chemical solution.
[0007]
In the first invention,
A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body has a main information entry part at the front part, and an edge part of the lid mounting part is provided with a sub information entry part corresponding to the sample storage part,
The lid is formed so as not to cover the sub information entry part when it is attached to the lid attachment part of the cassette body .
This is a cassette for pathological examination.
[0008]
In the second invention,
A cassette for histopathology examination,
The cassette body,
A lid that is detachably attached to the cassette body;
With
When the bottom of another cassette body is stacked on the closed cassette, between the lower cassette and the upper cassette body,
The spacer provided on the top side of the lid, the spacer provided on the bottom of the cassette body, the notch provided on the side of the lid protruding from the top of the cassette, or the width of the lid is made narrower than the width of the cassette body. opening provided on both sides, or those ones were combined, characterized in that in having a liquid passing part is formed,
This is a cassette for pathological examination.
[0009]
In the third invention,
A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body or the lid body is provided with a liquid passage forming means that allows liquid to flow even in a stacked portion when another cassette is stacked,
The liquid passing portion forming means includes a spacer provided on the upper surface side of the lid, a spacer provided on the bottom of the cassette body, a notch provided on the side of the lid protruding from the upper surface of the cassette, or a width of the lid. An opening formed narrower than the width of the main body and provided on both sides, or a combination of these,
When another cassette body is stacked on the upper surface of the lid, a gap is formed between the lower cassette and the upper cassette body so that the liquid can be passed by the fluid passage forming means. ,
This is a cassette for pathological examination.
[0010]
In the fourth invention,
A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body has a main information entry part at the front part, and an edge part of the lid mounting part is provided with a sub information entry part corresponding to the sample storage part,
The lid is formed so as not to cover the sub information entry part when attached to the lid attachment part of the cassette body,
The upper surface of the lid is provided with a spacer that forms a gap in the stacked portion when another cassette is stacked ,
Cassette for pathological examination.
[0012]
The location where the information entry portion is provided is not particularly limited as long as it is on the upper surface side of the cassette body, and may be provided on the edge portion on the upper surface side or may be provided in the central portion.
Examples of the liquid passage forming means include a spacer provided on the top surface of the lid, a spacer provided on the bottom of the cassette body, a notch provided on the side of the lid protruding from the top of the cassette, a lid For example, the body width is narrower than the width of the cassette body and openings are provided on both sides, or a combination of them.
[0013]
(Function)
An information entry portion corresponding to the specimen storage portion is provided on the upper surface side of the cassette body, and the lid is formed so as not to cover the information entry portion when attached to the lid attachment portion of the cassette body. If there is a sample, the information can be entered for each specimen storage section with the cassette body placed with the bottom face down, so it is easy to fill in and the specimen stored in the specimen storage section. There is no worry of spilling even when the lid is open. Moreover, since the information entry part can be seen from the upper surface side, the content of information is easy to visually recognize.
Therefore, if containing the sample of different types, each specimen storage section, can identify the specimen by the information entry for each specimen containing portion, it is possible to prevent a mistake of the specimen to be examined.
[0014]
When a stack of cassettes, in a to be a liquid permeation possible in a stacked portion, when immersed stacking multiple cassettes in a work of immersing the cassette in the chemical also the internal liquid medicine each cassette by passing liquid portion Circulate smoothly. Therefore, the chemical solution enters the inside of each cassette quickly, and the working efficiency is improved.
[0015]
DETAILED DESCRIPTION OF THE INVENTION
The invention will be explained in more detail on the basis of an embodiment shown in the drawing.
1 is an exploded perspective view showing an embodiment of a cassette according to the present invention, FIG. 2 is a cross-sectional view corresponding to the A-A portion in FIG. FIG. 3 is a cross-sectional explanatory view showing an engaged state of the engaging portion of the cassette body and the engaging claw of the lid.
Reference numeral C denotes a cassette according to the present invention, which is made of a synthetic resin having chemical resistance. The cassette C includes a cassette body 1 and a lid 2 that can be attached to and detached from the cassette body 1.
[0016]
On the upper surface side of the cassette body 1, an upper surface plate 11 formed slightly lower than the peripheral edge portion 10 is divided and provided in two places in the width direction via a liquid passage port 110.
Between the both edges in the width direction of the upper surface plate 11 and the long sides of the peripheral edge 10, a sub information entry part 12 formed of an inclined surface is provided. The sub-information entry part 12 is formed with a slightly rough surface like a surface of ground glass so that the subject name, number, etc. can be entered with a pencil or the like.
[0017]
Each sub-information entry unit 12 is provided on the outside along a specimen storage unit 14 described later. Each sub information entry part 12 is provided with air vent holes 13 penetrating in two places. The air vent 13 prevents air from accumulating on the back side of the sub-information entry part 12 and the side plate having a mountain-like cross section when immersed in a chemical solution such as paraffin.
In addition, the inside of the boundary line 111 which is a boundary part of each said upper surface board 11 and each sub-information entry part 12 is the lid | cover mounting part 112 which mounts the cover body 2 mentioned later.
[0018]
Each upper surface plate 11 is provided with three specimen accommodating portions 14 at six places in total. The specimen storage unit 14 has an octagonal shape in plan view, and has a storage unit wall 140 that bulges downward. The accommodating portion wall 140 is provided with a large number of liquid passage holes 141 through which a chemical solution or the like can pass.
In addition, the liquid passage hole 141 can be provided so as to be perpendicular to the wall surface of each accommodating portion wall 14, and the hole direction is perpendicular from the viewpoint of facilitating die cutting and shortening the molding process. It can also be formed.
[0019]
As described above, the liquid passage port 110 is provided between the rows of the specimen storage portions 14 provided on the one side three. The liquid passage port 110 is for allowing a chemical solution to pass through a large amount efficiently. Further, a reinforcing beam 15 for reinforcing adhesion with paraffin is provided below the liquid passage port 110.
Further, a main information entry section 16 formed of an inclined surface is provided on the front side of the cassette body 1. The main information entry unit 16 is formed with a slightly rough surface like a surface of ground glass, like the sub information entry unit 12. The side surfaces 160 on both sides of the cassette body 1 are similarly finished so that writing is possible.
[0020]
An engagement step portion 17 is provided on the rear end surface of the cassette body 1 over the entire width. On the rear end surface of the cassette body 1, guide portions 18 are provided at two locations. Each guide portion 18 is provided by cutting away the upper surface side obliquely corresponding to the position of an engaging claw of the lid 2 described later. Note that an engagement portion 19 is provided on the back side of the main information entry portion 16 on the front side of the cassette body 1 to be engaged with a hooking claw of the lid 2 described later.
[0021]
The lid body 2 has a rectangular substrate 20 that can be fitted and mounted on the lid mounting portion 112 of the cassette body 1. When the lid body 2 is mounted on the lid mounting portion 112, the sub information entry section 12 of the cassette body 1 is provided. It is formed so as not to cover.
At both ends in the length direction of the substrate 20, spacers 21 and 22 having an L-shaped cross section are provided so as to protrude upward over the entire length in the width direction. The spacers 21 and 22 are formed at a height that slightly protrudes upward from the peripheral edge portion 10 when the lid body 2 is attached to the lid attachment portion 112.
The spacer 21 on the front side is provided with a hooking claw 23 at the center. Further, a knob 24 is provided on the spacer 22 on the rear side. The knob 24 is provided with two engaging claws 25 facing downward.
[0022]
The substrate 20 is provided with storage lids 26 at six locations corresponding to the specimen storage unit 14. Each housing lid portion 26 has an octagonal shape in plan view and has a lid portion wall 260 that bulges upward. The lid wall 260 is provided with a large number of liquid passage holes 261 through which a chemical solution or the like can pass, and four protrusions 262 are provided on the upper surface. These protrusions 262 are formed to be the same height as the spacers 21 and 22. In addition, between the rows of the storage lid portions 26, a liquid passage port 27 including a required number of relatively large holes is provided.
[0023]
(Work)
The operation of the cassette C according to the present invention will be described with reference to FIGS.
Since both the cassette body 1 and the lid 2 are formed of a synthetic resin having chemical resistance, the cassette C is not affected by the chemical used until the test specimen is completed.
When the lid 2 is attached to the cassette body 1 and integrated, the six specimen storage spaces 3 (shown in FIG. 2) are partitioned and independent while having liquid permeability.
[0024]
A sub-information entry unit 12 is provided at a position corresponding to the sample storage unit 14 at the edge of the lid mounting unit 112 of the cassette body 1, and when the lid 2 is mounted on the lid mounting unit 112 of the cassette body 1. Since the main information entry unit 16 and the sub information entry unit 12 are formed so as not to be covered, information can be entered for each specimen storage unit 14, and the information content of each information entry unit 12 is also Easy to see.
Therefore, when different types of specimens are accommodated in the specimen accommodating sections 14, it is possible to reliably identify the specimen by entering information for each specimen accommodating section 14, and to prevent mistakes in the specimen to be examined. .
[0025]
The lid 2 has an engaging claw 25 for engaging with the cassette body 1, and the engaging claw 25 is formed so as to engage with the engaging step portion 17 of the cassette body 1 from the outside. Therefore, when the operation of pulling up the end edge of the knob 24 is performed, the end edge of the lid body 2 is bent upward (see FIG. 3, phantom line portion), and the engaging claw 25 is opened outward. The engagement with the engagement step portion 17 is easily released.
Therefore, since the removal of the lid 2 can be performed gently without causing an impact, it is possible to prevent the specimen from popping out when the specimen block is taken out from the specimen storage section 14 or dropping the cassette C together with the specimen.
[0026]
Further, when the cassette C is stacked on the upper surface portion of the lid 2, spacers 21 and 22 and protrusions 262 that form a gap S (see FIG. 2) through which the liquid can pass are provided. Even when many cassettes C are stacked and immersed in the operation of immersing C in the chemical solution, the chemical solution smoothly circulates through the gap S. Accordingly, the chemical solution enters the inside of the cassette C quickly, and the working efficiency is improved.
[0027]
Furthermore, since the accommodating part wall 140 of the specimen accommodating part 14 has a bulging shape, the area becomes wider as long as the projection area is the same as that of a flat plate. Thereby, the number of the liquid passage holes 141 can be increased, the liquid permeability of the chemical liquid is improved, and the liquid passage amount is also increased.
[0028]
When the paraffin block is formed by the above process, the liquid inlets 110 and 27 function as a molten paraffin runner, and the molten paraffin easily flows into every corner of the embedding dish. Further, the reinforcing beam 15 below the liquid passage port 110 is fixed while being embedded in the paraffin when the paraffin is cured. As a result, the paraffin and the cassette body 1 are firmly fixed, and the connection force between the paraffin in the cassette body 1 and the paraffin outside the cassette body 1 is improved.
[0029]
Since the boundary surface between the paraffin inside the cassette body 1 of the paraffin block and the paraffin outside the cassette body 1, that is, the storage wall 140 of the specimen storage section 14 is an uneven surface, it is strong against the lateral force during the slicing operation. Even in this respect, it is possible to prevent the paraffin from falling off the cassette body 1.
[0030]
Note that the terms and expressions used in the present specification are merely explanatory and are not restrictive, and do not exclude terms and expressions equivalent to the above terms and expressions. The present invention is not limited to the illustrated embodiment, and various modifications can be made within the scope of the technical idea.
[0031]
【The invention's effect】
The present invention has the above configuration and has the following effects.
(A) An information entry portion corresponding to the sample storage portion on the upper surface side of the cassette body is provided, and the lid is formed so as not to cover the information entry portion when attached to the lid attachment portion of the cassette body. In the cassette, the information can be entered in the state where the cassette body is placed with the bottom face down when entering information for each specimen container. There is no risk of spilling even when the specimen is open. Moreover, since the information entry part can be seen from the upper surface side, the content of information is easy to visually recognize.
Therefore, if containing the sample of different types, each specimen storage section, can identify the specimen by the information entry for each specimen containing portion, it is possible to prevent a mistake of the specimen to be examined.
[0032]
(B) In the also possible liquid passing in a stacked portion of the time a stack of cassettes, when immersed stacking multiple cassettes in a work of immersing the cassette in the chemical also chemical each by passing liquid portion Distributes smoothly inside the cassette. Therefore, the chemical solution enters the inside of each cassette quickly, and the working efficiency is improved.
[0033]
(C) A main information entry part and a sub information entry part corresponding to the specimen storage part on the upper surface side of the cassette body are provided, and the sub information entry part is provided when the lid is attached to the lid attachment part of the cassette body. If the lid is provided with a spacer that allows liquid to pass through the stacked portion when the cassettes for pathological examination are stacked, the above (a) , (B) can be provided.
[Brief description of the drawings]
FIG. 1 is an exploded perspective view showing an embodiment of a cassette according to the present invention.
FIG. 2 is a cross-sectional view corresponding to the AA portion in FIG.
FIG. 3 is an explanatory cross-sectional view showing an engaged state of an engaging portion of a cassette body and an engaging claw of a lid.
FIG. 4 is a perspective view showing an example of a conventional cassette.
[Explanation of symbols]
C Cassette 1 Cassette body 10 Peripheral part 11 Top plate 110 Fluid inlet 12 Sub-information entry part 13 Air vent hole 14 Sample container part 111 Boundary line 112 Lid mounting part 140 Container part wall 141 Fluid hole 15 Reinforcement beam 16 Main information entry Part 17 Engagement step part 18 Guide part 19 Engagement part 2 Lid body 20 Substrate 21 and 22 Spacer 23 Hook claw 24 Pick piece 25 Engagement claw 26 Storage lid part 260 Lid wall 261 Fluid passage hole 262 Projection 27 Fluid passage opening

Claims (4)

A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body has a main information entry part at the front part, and an edge part of the lid mounting part is provided with a sub information entry part corresponding to the sample storage part,
The lid is formed so as not to cover the sub information entry part when it is attached to the lid attachment part of the cassette body.
Cassette for pathological examination. A cassette for histopathology examination,
The cassette body,
A lid that is detachably attached to the cassette body;
With
When the bottom of another cassette body is stacked on the closed cassette, between the lower cassette and the upper cassette body,
The spacer provided on the top side of the lid, the spacer provided on the bottom of the cassette body, the notch provided on the side of the lid protruding from the top of the cassette, or the width of the lid is made narrower than the width of the cassette body. opening provided on both sides, or those ones were combined, characterized in that in having a liquid passing part is formed,
Cassette for pathological examination. A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body or the lid body is provided with a liquid passage forming means that allows liquid to flow even in a stacked portion when another cassette is stacked,
The liquid passing portion forming means includes a spacer provided on the upper surface side of the lid, a spacer provided on the bottom of the cassette body, a notch provided on the side of the lid protruding from the upper surface of the cassette, or a width of the lid. An opening formed narrower than the width of the main body and provided on both sides, or a combination of these,
When another cassette body is stacked on the upper surface of the lid, a gap is formed between the lower cassette and the upper cassette body so that the liquid can be passed by the fluid passage forming means. ,
Cassette for pathological examination. A cassette for histopathology examination,
A cassette body having a required number of sample storage portions having a lid mounting portion on the upper surface side and a liquid passage hole on the bottom portion;
A lid body that is detachable from the lid mounting portion of the cassette body and has a liquid passage hole;
The cassette body has a main information entry part at the front part, and an edge part of the lid mounting part is provided with a sub information entry part corresponding to the sample storage part,
The lid is formed so as not to cover the sub information entry part when attached to the lid attachment part of the cassette body,
The upper surface of the lid is provided with a spacer that forms a gap in the stacked portion when another cassette is stacked,
Cassette for pathological examination.

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