JP2003517609A - 等温変性条件下での、標的種に結合する化合物を同定するための蛍光相関分光検査法の使用 - Google Patents

等温変性条件下での、標的種に結合する化合物を同定するための蛍光相関分光検査法の使用

Info

Publication number
JP2003517609A
JP2003517609A JP2001545854A JP2001545854A JP2003517609A JP 2003517609 A JP2003517609 A JP 2003517609A JP 2001545854 A JP2001545854 A JP 2001545854A JP 2001545854 A JP2001545854 A JP 2001545854A JP 2003517609 A JP2003517609 A JP 2003517609A
Authority
JP
Japan
Prior art keywords
target species
test
target
protein
denaturing conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2001545854A
Other languages
English (en)
Japanese (ja)
Other versions
JP2003517609A5 (enExample
Inventor
イー． エップス，デニス
ケー． トミク，ポール
ジェイ． ケズディー，フェレンク
ケー． マーシュク，チャールズ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Pharmacia and Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia and Upjohn Co filed Critical Pharmacia and Upjohn Co
Publication of JP2003517609A publication Critical patent/JP2003517609A/ja
Publication of JP2003517609A5 publication Critical patent/JP2003517609A5/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/12—Condition responsive control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2001545854A 1999-12-09 2000-12-07 等温変性条件下での、標的種に結合する化合物を同定するための蛍光相関分光検査法の使用 Pending JP2003517609A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/458,172 1999-12-09
US09/458,172 US6582907B1 (en) 1999-12-09 1999-12-09 Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
PCT/US2000/042642 WO2001044817A2 (en) 1999-12-09 2000-12-07 Fluorescence correlation spectroscopy to identify compound-target binding

Publications (2)

Publication Number Publication Date
JP2003517609A true JP2003517609A (ja) 2003-05-27
JP2003517609A5 JP2003517609A5 (enExample) 2005-12-22

Family

ID=23819671

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001545854A Pending JP2003517609A (ja) 1999-12-09 2000-12-07 等温変性条件下での、標的種に結合する化合物を同定するための蛍光相関分光検査法の使用

Country Status (13)

Country Link
US (2) US6582907B1 (enExample)
EP (1) EP1236047B1 (enExample)
JP (1) JP2003517609A (enExample)
AT (1) ATE265684T1 (enExample)
AU (1) AU777163B2 (enExample)
CA (1) CA2392239A1 (enExample)
DE (1) DE60010323T2 (enExample)
DK (1) DK1236047T3 (enExample)
ES (1) ES2218280T3 (enExample)
HK (1) HK1047158B (enExample)
PT (1) PT1236047E (enExample)
TR (1) TR200401075T4 (enExample)
WO (1) WO2001044817A2 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6517781B1 (en) * 1997-06-02 2003-02-11 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
DE10013854A1 (de) * 2000-03-17 2001-09-20 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
AU2003244122A1 (en) * 2002-06-21 2004-01-06 Olympus Corporation Biomolecule analyzer
EP1527333A1 (en) * 2002-08-01 2005-05-04 Sensor Technologies LLC Fluorescence correlation spectroscopy instrument
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2006013109A1 (en) * 2004-08-06 2006-02-09 Epfl Swiss Federal Institute Of Technology-Lausanne Method for testing a substance interacting with a target molecule
EP1848983B1 (en) * 2005-02-15 2013-03-27 Tata Institute of Fundamental Research Fluorescence correlation microscopy with real-time alignment readout
DE102007045666A1 (de) * 2007-09-25 2009-04-02 Carl Zeiss Microimaging Gmbh Verfahren und Anordnung zur Ausgabe von Restfehlern einer an eine Punktemenge angepassten Funktion
KR100963305B1 (ko) 2008-01-23 2010-06-11 건국대학교 산학협력단 단백질 내의 도메인 간 변성과정 분석방법
US12400741B2 (en) * 2020-02-28 2025-08-26 Howard University Selective extraction of late exponentials

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585277A (en) 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
CA2236339A1 (en) 1995-12-07 1997-06-12 Robert T. Sauer A fluorescence-based screening method for identifying ligands
HUP9902418A3 (en) 1996-05-09 2001-10-29 Dimensional Pharm Inc Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
EP0836090A1 (en) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Method of analysis of samples by determination of the distribution of specific brightnesses of particles
AU738194B2 (en) 1997-12-05 2001-09-13 Pharmacia & Upjohn Company Fluorescence-based high throughput screening assays for protein kinases and phosphatases
US6242190B1 (en) 1999-12-01 2001-06-05 John Hopkins University Method for high throughput thermodynamic screening of ligands
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions

Also Published As

Publication number Publication date
EP1236047B1 (en) 2004-04-28
US20030219820A1 (en) 2003-11-27
EP1236047A2 (en) 2002-09-04
AU4519801A (en) 2001-06-25
DK1236047T3 (da) 2004-07-05
DE60010323D1 (de) 2004-06-03
DE60010323T2 (de) 2005-05-25
HK1047158A1 (en) 2003-02-07
WO2001044817A3 (en) 2002-05-10
AU777163B2 (en) 2004-10-07
PT1236047E (pt) 2004-08-31
ATE265684T1 (de) 2004-05-15
WO2001044817A2 (en) 2001-06-21
CA2392239A1 (en) 2001-06-21
ES2218280T3 (es) 2004-11-16
US6582907B1 (en) 2003-06-24
TR200401075T4 (tr) 2004-07-21
HK1047158B (en) 2004-12-03

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Effective date: 20040708