JP2003325149A - Water-soluble nucleoprotein-containing healthy drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a water-soluble nucleoprotein-containing healthy drink into which the water-soluble nucleoprotein obtained by subjecting soft roe to enzymatic hydrolysis treatment and therefore having a low molecular weight is mixed together with other active ingredients in a suitable ratio. <P>SOLUTION: This water-soluble nucleoprotein-containing healthy drink contains active ingredients comprising the water-soluble nucleoprotein, collagen, chondroitin, hyaluronic acid, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12 in suitable amounts based on a specified amount of the healthy drink, other admixture components, and water, wherein the water-soluble nucleoprotein is obtained by decreasing the molecular weight of the nucleoprotein in the soft roe of fish so as to be water-soluble. The healthy drink makes nucleic acids and proteins consisting mainly of arginine effectively digested and assimilated when drunk, so that the healthy drink reduces oxidative damage of the gene and is useful for maintenance/promotion of the human health. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a health drink containing a water-soluble nucleoprotein, and more specifically, a low-molecular-weight water-soluble nucleoprotein obtained by enzymatically decomposing fish testis shirako. By reducing the oxidative damage of the gene, and by expressing the synergistic effect of each active ingredient by optimally adjusting the mixing ratio of the water-soluble nucleoprotein and other active ingredients, thereby, in the conventional homogeneous health drink In comparison, the present invention relates to a health drink containing water-soluble nucleoprotein, which has a further improved effect of maintaining and promoting human health.

[0002]

2. Description of the Related Art In recent years, public interest in health has increased, and in addition to conventional nutrients, nucleic acid has been attracting attention as a seventh nutrient. Examples of foods containing a large amount of nucleic acids include brewer's yeast and Shirako (testis of fish). Shirako is a food rich in useful components such as nucleic acids and minerals.

However, nucleic acids and protein peptides mainly composed of arginine in algae have not hitherto been effectively used as a component of drinks, despite their excellent food effects. Because nucleoprotein is a polymer that is difficult to dissolve in water, the dosage form of products using nucleoprotein has heretofore been limited to tablets, granules and capsules.

Recently, it has been found that water-soluble nucleoprotein has an effect of reducing oxidative damage of genes. Gene oxidation induces gene degradation and damage. Reflecting growing public interest in environmental assessment, eliminating or suppressing oxidative damage to genes received from the living environment as much as possible is considered to be extremely important for maintaining and improving human health. became.

The harmful effects of oxidative damage on genes are explained below. The genetic information of an organism is such that adenine, thymine,
It is no longer known that it is encoded by the four bases of guanine and cytosine. However, genes can be oxidatively damaged at a certain frequency due to ultraviolet irradiation, exposure to various chemical substances, especially carcinogens and reactive oxygen species, and replication errors can occur. This replication error causes deterioration of genetic information, which causes various diseases and aging of cells and individuals. Therefore, by examining whether a substance promotes or suppresses the oxidative damage of a gene, it is possible to judge whether the substance has a toxicity or an effect on an organism. .

[0006] As a biological evaluation method for substances by determining the frequency of causing gene damage, International Publication 01
Examples thereof include those described in Japanese Patent No. 69235. In this method, focusing on the oxidation of mononucleosides that make up the gene, 2'-deoxyguanosine (dG) was added to the solution containing the test substance, and the oxidative load such as the passage of a certain period of time and / or ultraviolet irradiation was increased. D) present in the solution after addition
The concentration of the oxidant of G: 8-hydroxy-2′-deoxyguanosine (8OHdG) is measured. Then, based on the amount of generated 8OHdG, the presence or absence and the degree of toxicity or effectiveness of the test substance are determined. The higher the amount of 8OHdG produced, the higher the toxicity of the test substance.
It is considered that the smaller the amount of OHdG produced, the higher the effectiveness of the test substance.

[0007]

Among the conventional health drinks that have been considered to be effective for maintaining and promoting health according to the recent research based on the above-mentioned biological evaluation method for measuring the amount of 8OHdG produced. It was found that there are not a few substances containing a component having oxidative damage to genes. Therefore, if an active ingredient that reduces oxidative damage to genes is added to a health drink, it may be possible to impart a new effect to the health drink, which is not available in conventional health drinks. It was highly anticipated. Also, conventionally, the relationship between the effect and the change in the type of active ingredient or the blending ratio thereof in health drinks has not always been clear. It is considered that the overall effect as a health drink will change depending on the type of active ingredient and the change of their compounding ratio. However, in order to clarify the relationship between the effect of changes in the types of active ingredients of health drinks and their mixing ratios and their effects, for example, a large number of people are allowed to taste multiple types of test health drinks, and appropriate A complicated method such as conducting a questionnaire survey before and after tasting is required for each item, and implementation is not always easy. Therefore, it has been sought to provide a health drink which is effective for maintaining and promoting human health, in which the above-mentioned matters are clarified and each active ingredient is blended so as to obtain the optimum effect. .

The present invention is intended to solve the above-mentioned problems of the prior art, and its purpose is to combine with the components which have been known to be effective for maintaining and promoting human health in the past. The present invention also provides a health drink that also contains a water-soluble nucleoprotein that reduces oxidative damage to protein genes, and that the types and mixing ratios of these active ingredients are optimally adjusted.

[0009]

[Means for Solving the Problems] That is, the first aspect of the present invention is a health drink comprising 720 ml of the health drink.
Water-soluble nuclear protein 700 mg to 20000 mg, collagen 40 g to 150 g, chondroitin 50 mg to 200 mg, hyaluronic acid 20 mg to 80 mg, vitamin C 500 mg to 14000 mg, vitamin B1 2 mg to 40 mg, vitamin B2 3 mg to 50 mg, vitamin B6 3 mg to 50 mg, Vitamin B12 0.3 μg to 7.0 μg and other additive components and the balance water, and the water-soluble nucleoprotein is a water-soluble nucleoprotein which is made water-soluble by reducing the molecular weight of the nucleoprotein in fish alga. The present invention relates to a health drink containing water-soluble nucleoprotein (hereinafter referred to as "i").

The second invention is a health drink,
Water-soluble nuclear protein 1000 mg-15000 mg, collagen 80 g-120 g, chondroitin 80 mg-150 mg, hyaluronic acid 30 mg-60 mg, vitamin C 600 mg-10000 mg, vitamin B1 10 mg-30 mg, vitamin B2 15 mg-40 mg, vitamin B6 per 720 ml of the health drink. 15 mg to 40 mg, Vitamin B12 1.5 μg to 5.0 μg and other added components and the balance water, and the water-soluble nucleoprotein is made water-soluble by reducing the molecular weight of nucleoprotein in fish algae. The present invention relates to a health drink containing a water-soluble nucleoprotein (hereinafter referred to as ii), which is a nucleoprotein.

The water-soluble nucleoprotein-containing health drink of the present invention preferably has the following embodiments. iii) A health drink containing a water-soluble nucleoprotein according to item i or ii, wherein the water-soluble nucleoprotein is made to be water-soluble by lowering the molecular weight of the nucleoprotein in salmon, trout, or herring albino. iv) The water-soluble nucleoprotein contains the water-soluble nucleoprotein of any one of i to iii, which contains 20% by mass or more of a low molecular weight oligonucleotide / nucleoside and oligopeptide obtained by treating the nucleoprotein with a nuclease and a protease. Healthy drink. v) Active ingredients other than the active ingredient of the health drink of i or ii, which are vitamins, minerals, amino acids, peptides, saccharides (for example, fructose, glucose, maltose, etc.), water-soluble plant fibers, fruits or A health drink containing water-soluble nucleoprotein i or ii, further comprising at least one selected from vegetable juice, honey, flavoring agents, preservatives and coloring agents.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The water-soluble nucleoprotein, which is the active ingredient of the health drink of the present invention, can be obtained from the nucleoprotein in the agar of fish. The water-soluble nuclear protein will be described below. The water-soluble nucleoprotein can be produced from Miko (testis of fish). Shirako is an underutilized marine resource that is currently only partially used for food and mostly discarded. But Shirako is DN
It was found that it contains a large amount of a nucleic acid containing A as a main component and a protein containing protamine as a main component, and this has an effect of significantly reducing oxidative damage of a gene.

In the water-soluble nuclear protein, the molecular weight of the nuclear protein is reduced. The low-molecular-weight product is a water-soluble nuclear protein containing 20% by mass or more of an oligonucleotide / nucleoside and an oligopeptide. By lowering the molecular weight of nucleic acids and proteins in the cell nucleus in this way, digestion and absorption in the digestive tract can be facilitated while maintaining the original effectiveness. Since the low molecular weight nuclear protein becomes water-soluble, it can be easily incorporated into a health drink. The water-solubility referred to here is a property of being soluble in water at a concentration of 0.1% by mass or more.

The water-soluble nuclear protein thus obtained is
It has a good inhibitory effect on gene oxidative damage. Gene oxidative damage causes deterioration of genetic information encoded in genes and causes various diseases and aging, but water-soluble nucleoproteins cause oxidative damage when oxidative load is applied to genes. Has the effect of significantly suppressing

The usefulness of the water-soluble nucleoprotein will be described in more detail below. It is known that the cell nucleus of fish contains a large amount of protamine, which is a protein containing arginine as a main component, and an oligopeptide obtained by degrading protamine exhibits excellent effects such as antiallergic action and skin beautifying action. Nucleic acid has the effect of preventing and improving beautiful skin, lifestyle-related diseases (adult diseases) and aging. Therefore, by producing low molecular weight nucleoprotein (water-soluble nucleoprotein) using Shirako as a raw material,
It is possible to obtain a product which has an excellent effect of suppressing gene oxidative damage and also has an effect derived from nucleic acid and protamine. Examples of the fish include salmon, trout, herring, cod, and the like, and salmon, trout, and herring are particularly preferable. Skin, muscle,
The water-soluble nuclear protein can be produced by removing blood vessels and the like, removing the oil by purification, and performing an enzymatic decomposition treatment with a nuclease and a protease.

The water-soluble nuclear protein used in the present health drink preferably contains 20% by mass or more of a low molecular weight oligonucleotide / nucleoside and oligopeptide obtained by treating the nuclear protein with nuclease and protease.

Each component of the health drink will be described below. I) Water-soluble nucleoprotein "Nucleic acid" which plays an important role in life phenomenon exists in the cell nucleus in cells of all organisms, and there are two types of nucleic acid, DNA and RNA. (1) DNA (deoxyribonucleic acid) After a growing period, the DNA production capacity is drastically reduced. DNA conveys genetic information as the body of a gene, controls all of cell division, growth, and energy production, and controls cell birth to death. DNA has a structure in which pentose, phosphate, and four kinds of bases (A, G, C, T) are connected in a spiral shape, and is made in the liver (de novo synthesis) and ingested from food. Two types are utilized in the body, one in which nucleic acid is resynthesized (salvage synthesis).
However, after the growth period, the ability to produce in the liver declines,
Slow cell division, poor quality cells, or incomplete cells are created. It causes adult diseases and aging, and appears in various symptoms. Therefore, it is necessary to supplement the amount of DNA commensurate with the reduced production capacity.

(2) Function of DNA 1) Transmission of genetic information All living organisms make a body by vigorously dividing cells during the growth process. Once the body is complete, it will continue to replace the old cells with new ones. Fine D
NA is the same DN as myself in cell division
It makes A and nuclei, and by its command, it works to make exactly the same cells as old cells. 2) Protein synthesis DNA and RNA make a protein (body). Heart, liver,
The main components of the body, including the skin, are proteins. In addition, the enzymes that are involved in various chemical reactions that occur in the living body are also proteins. These proteins are RNA by the instruction of the DNA that all genetic information is input.
(Ribbonucleic acid) is made by combining amino acids. However, if even one combination of amino acids is different, it will become an incomplete protein and its original function will not work, or normal chemical reaction will not be possible. As a result, blood, skin, hair and internal cells are altered, causing aging and adult diseases.

<Main effects of DNA (nucleic acid)> a. The first effect that comes from ingesting nucleic acid is that the facial expression changes. This is because the skin begins to become tense and wrinkles and tarmi are removed, and the spots gradually become less noticeable. It also moisturizes dry, dry skin due to aging. b. It restores the normal state of metabolic abnormalities such as the oversecretion of fat that is the cause of acne. c. Hair loss and gray hair are caused by the slowdown of cell division in the hair bulb part and its abnormality. By promoting correct cell division, hair loss and gray hair are prevented. d. Improves respiratory function, enhances lung function, and prevents fatigue. e. It prevents the increase of cholesterol and helps prevent arteriosclerosis. f. It increases cerebral blood flow and oxygen consumption, and reduces cerebrovascular resistance. Effective in improving aftereffects of stroke and encephalomalacia. g. Since it increases coronary blood flow and myocardial metabolism, it is effective in preventing heart failure, angina and cardiomyopathy. h. Increases gastric blood flow and activates gastrointestinal function. It also has the effect of promoting bowel movements. i. It is effective in preventing aging of the brain, improving eyestrain and hearing loss. j. In addition, the underlying causes of diseases are fundamentally improved in terms of cell activation.

<Ingestion of DNA (nucleic acid)> Ingestion of DNA nucleic acid (nuclear protein) may not be so effective for young people who have just passed the growth period. However, aging has already been prepared since the teens. Therefore, supplementing nucleic acid (nuclear protein) from a young age and maintaining and promoting correct cell division are the best ways to prevent aging. As a food containing a lot of nucleic acids (nuclear proteins),
Shirako and brewer's yeast. However, these foods are generally not eaten well. Even if I try to eat it again, there are still problems with the taste and storage method. That is why it becomes necessary to take it in the form of dietary supplements.
The water-soluble nucleoprotein used in the health drink of the present invention was developed so that the nucleoprotein can be processed into water by enzymatic treatment and added to the drink. This healthy drink 720
700 ml to 20,000 m of water-soluble nuclear protein per ml
It is preferable to add g, particularly 1000 mg to 15000 mg.

II) Collagen Collagen is the most abundant protein in the animal body.
It accounts for 20% by mass to 40% by mass of all proteins in the living body. Collagen exists as a fibrous protein that fills the spaces between cells of the body and is a major constituent of connective tissues such as skin, bone, cartilage, and tendon. 40% by mass of the total collagen amount is contained in the skin, 20% by mass is contained in bone and cartilage, and is widely distributed throughout the body such as blood vessels and internal organs. Thus, collagen acts as a structural material that supports the shape of the body and organs, and also plays the role of an adhesive as a scaffold (matrix) that connects cells together. In addition to these physical functions, collagen has been found to have a great influence on biological activities such as cell proliferation, organ formation, and wound healing promotion. On the other hand, collagen is produced in fibroblasts, chondrocytes, osteoblasts, etc.
These are relationships that influence each other.

<Relationship between collagen and aging> In the human body, collagen is constantly decomposed and synthesized repeatedly.
As we get older, this balance is upset and more degradation occurs than collagen synthesis. This is one of the aging phenomena. Collagen loses its flexibility, causing wrinkles and spots, and joints and bones. Therefore, in order to counter aging, it is necessary to supplement collagen to promote metabolism.

<Existence Form of Collagen> From a microscopic view of collagen, three spiral elongated molecules are gathered to form a more confused structure. Collagen does not dissolve in water as it is, but when it is heated with water for a long time, at a certain temperature, three chains are detached and the structure changes to dissolve in water, and it is extracted into the liquid. This is called gelatin. Although the shape is different, the same thing is practiced in daily cooking such as stewed boiled meat and stewed meat, pork bone soup, etc. Therefore, it can be said that eating a food rich in collagen has the same meaning as eating gelatin in some form.

<Meaning of collagen as food> In recent years,
The health and beauty effects of eating collagen have been the subject of discussion, but in general, "collagen" in the food market refers to "gelatin" or "collagen peptide" that is a low molecular weight gelatin. It has been reported that collagen intake is effective in preventing hypertension, reducing osteoporosis, and healing arthritis. It has also been reported that it promotes collagen synthesis in the body to rejuvenate the skin and enhances the moisturizing properties of the skin. With the advent of an aging society, the importance of collagen is expected to become even more important. This 720ml health drink has 40-150g collagen, especially 80-12g collagen.
It is preferable to add 0 g.

III) Chondroitin for Food Chondroitin is also called a chondroitin protein complex. It is a natural product that extracts proteins such as mucopolysaccharides such as chondroitin sulfate and collagen, which are abundant in connective tissues and cartilage of animals, in a natural form. It has been purified to. It is also called mucopolysaccharide in a broad sense. More specifically, the mucopolysaccharide has an acidity and a neutrality, and the neutral mucopolysaccharide corresponds to a highly viscous substance or a blood-forming substance that covers the mucous membrane of the stomach. On the other hand, animal eyes, cartilage, skin,
Most of the highly viscous substances in connective tissues such as tendons, blood vessel walls, ligaments and umbilical cords are acidic mucopolysaccharides.

The physiological actions of food chondroitin are as follows.・ Adjusting the water content of extracellular fluid.・ Fossil formation.・ Wound healing.・ Prevention of infection.・ Lubrication. -Prevents blood coagulation. -Fat serum purification.・ Maintains transparency of eyes. 50 parts of chondroitin per 720 ml of this health drink
It is preferable to add mg to 200 mg, particularly 80 mg to 150 mg.

IV) Hyaluronic Acid <Function of Hyaluronic Acid> A type of highly viscous mucopolysaccharide, which is contained in animal ligaments, joints, vitreous humor of the eyeball, and the like. In the body, it creates a viscous solution or gel together with proteins, and has the function of connecting cells. In addition, it works to protect yourself from the ingress of lubricants and bacteria that prevent friction in joints. <Characteristics of hyaluronic acid> Hyaluronic acid has an extremely excellent moisturizing property and has a strong water retaining ability of holding about 6 liters of water per 1 g. Further, in order to form a protective film that gives a firm and smooth feel when applied to the skin, it is also incorporated in cosmetics and the like, in addition to foods, for example, as a skin roughening inhibitor and a skin wrinkle inhibitor. 20mg-80mg, especially 30mg of hyaluronic acid per 720ml of this health drink
It is preferable to add -60 mg.

V) Vitamin C The main functions of Vitamin C are as follows.・ Helps collagen synthesis and strengthens skin, blood vessels, mucous membranes and bones. -Antioxidative damage, vitamin E reduction action. -Prevention of arteriosclerosis. -Relief of chronic fatigue syndrome. -Suppression of Helicobacter pylori growth. -Blur prevention.・ Prevention of eye diseases such as presbyopia and cataract. -Suppress the production of the carcinogen nitrosamine. -Promotion of in-vivo synthesis of interferon which is an anti-cancer agent. -Participation in the conversion of cholesterol into bile acids.・ It has anti-cancer, anti-viral, and detoxifying effects because it strengthens the function of white blood cells.・ Helps to absorb minerals such as iron and copper, and helps to synthesize hemoglobin. -Involvement in the production of anti-stress hormone (adrenocortical hormone). 500m of vitamin C per 720ml of this health drink
g-14000 mg, especially 600 mg-10000 mg
It is preferable to add.

VI) Vitamin B1 Vitamin B1 is contained in 200 ml of milk in an amount of about 10% by mass of the required amount per day for human beings.
It is contained in many foods such as meat. However, when brown rice is 100 in rice, milled rice is as small as 19 and there is a tendency to consume too much favorite beverage recently, and it is said that children and young people in the growing season are deficient in vitamin B1. There is. The function of vitamin B1 is that after sugar is absorbed as glucose (glucose), apoenzyme (synthesized from protein) works to produce energy.
At this time, vitamin B1 and pantothenic acid act as coenzymes. It is said that when B1 is insufficient, it is easy to get tired, loses appetite, and is related to growth and nerve function.
2mg of vitamin B1 per 720ml of this health drink
-40 mg, especially 10 mg-30 mg is preferably added.

VII) Vitamin B2 Vitamin B2, which is also called "growth vitamin", is about 3 in 200 ml of milk against the daily requirement of elementary school students.
You can make up for 0%. In addition to growth, it is known to be related to the functions of the tongue, lips, skin, eyes, etc., and enhance motor skills. It is said that lack of vitamin B2 is associated with symptoms such as seborrheic dermatitis, cheilitis, and eye fatigue.
Recently, it is also called "beauty vitamin", and it can be said that this is a vitamin that children, young people, and women in the growing season should pay particular attention to. This healthy drink 720
Vitamin B2 is 3 mg to 50 mg per ml, especially 15
It is preferable to add mg to 40 mg.

VIII) Vitamin B6 Vitamin B6 functions mainly in the metabolism of proteins.
It also works by converting proteins to sugars and lipids, and vice versa. Since vitamin B6 has a function of normalizing immunity, it is used in combination with an antihistamine agent for the treatment of allergy, and also has a diuretic action and a detoxification action. It is also related to the function of lipids, and when a large amount of fat is eaten, vitamin B6 is consumed and deficient, and if it continues, fatty liver, cirrhosis, cholesterolemia, and arteriosclerosis may occur. In particular, alcoholics tend to be deficient in vitamin B6. In diabetes, vitamin B6 to improve insulin
is necessary. Furthermore, one of the causes of morning sickness is vitamin B.
It is considered to be 6 deficiency, and it is said that 6 times more vitamin B6 than usual is required during pregnancy, not only when the morning sickness is severe. B vitamins, including vitamin B6, are interrelated and working. This healthy drink 720
Vitamin B6 is 3 mg to 50 mg per ml, especially 15
It is preferable to add mg to 40 mg.

IX) Vitamin B12 Vitamin B12 is a vitamin whose function has been clarified since it was cured well in 1929 when a patient with pernicious anemia was given milk and gastric juice of a healthy person. As can be seen from this, in the growing period of human beings, the amount required per day is as large as 25% by mass to 50% by mass in 200 ml of milk. Vitamin B12 is known to cooperate with folic acid, which is often contained in green-yellow vegetables, to promote the synthesis of hemoglobin in erythrocytes, and to participate in the synthesis of nucleic acid, which is the main component of DNA (gene). Vitamin B12 deficiency leads to abnormal production of red blood cells, and because hemoglobin of red blood cells carries oxygen throughout the body, it causes insufficient energy production in the body. B12 is said to be closely related to nerves and is known to be involved in the repair of peripheral nerves and the brain of central nerves, and is also considered to be involved in memory and mental balance. Vitamin B12, like Vitamin B2, can be said to be a vitamin that should be taken into consideration especially during human growth. For 720 ml of this health drink,
Vitamin B12 0.3 μg to 7.0 μg, especially 1.5
It is preferable to add μg to 5.0 μg.

As the water used for the present health drink, pure water suitable for the health drink conventionally used in this field can be used. This health drink may contain vitamins other than the above-mentioned active ingredients (for example, vitamins D, E, and
K, etc., minerals (eg, Mg, Ca, Fe, C)
u, Zn, Se, etc.), amino acids, peptides, saccharides,
An active ingredient consisting of at least one selected from water-soluble vegetable fiber, fruit or vegetable juice, honey, flavor, preservative and colorant may be further added, and the addition amount in this case may be appropriately selected.

[0034]

The present invention will be described in more detail by the following examples. The water-soluble nucleoprotein, which is a raw material for the water-soluble nucleoprotein of this health drink, was produced as follows. <Production of Water-Soluble Nuclear Protein> 2500 g of frozen salmon Shirako was thawed to remove skins, muscles, blood vessels, etc., and then blood was removed and washed with water. Then, the salmon Shiroko was pulverized together with 1000 ml of water, 2.5 g of protease (manufactured by NOVO) was added, and enzyme treatment was carried out at 44 to 47 ° C. and pH 6.0 to 6.3 for 4 hours while stirring. Subsequently, the treated liquid is treated at 70 ° C.
The temperature was raised to 2.5 g, and 2.5 g of nuclease (manufactured by Amano Co., Ltd.) was added, and the enzymatic decomposition treatment was carried out for 4 hours at pH 5.0 to 5.5 while stirring. After the treatment, the temperature of the liquid was raised to 85 ° C. to deactivate the remaining protease and nuclease. The obtained product liquid is cooled to 40 to 50 ° C., continuously fed to a decanter to separate a clarified liquid and spray-dried, and in the form of powder, water-soluble low molecular weight nuclear protein I got a nuclear protein.
The properties of the obtained water-soluble nucleoprotein are as shown in Table 1. As the water-soluble nucleoprotein of the present health drink, this water-soluble nucleoprotein can be used as it is or, if necessary, further purified.

Using the water-soluble nucleoprotein and other active ingredients, a health drink of the following composition [for convenience, referred to as natural DN collagen] was produced. Two types of comparative health drinks having the following compositions were prepared (hereinafter referred to as nuclear protein drink Type A (comparative drink A) and (nuclear protein) collagen drink Type B (comparative drink B) for convenience). The composition of the present health drink corresponds to the composition of the compositions of the comparative drinks A and B, but the composition and content of each active ingredient are different between the present health drink and the comparative drinks A and B. That is, the comparative drink A or the comparative drink B does not contain the active ingredient of the present health drink, or contains the active ingredient in an amount outside the range of the active ingredient of the present health drink. In addition, in order to clarify the difference between the composition of this health drink and the compositions of comparative drinks A and B, regarding comparative drinks A and B, the presence or absence and the increase / decrease amount of each active ingredient based on the composition of this health drink Indicated.

<Health Drink: Natural DN Collagen Composition> Water-soluble nuclear protein 1440 mg, collagen 80 g, chondroitin 115 mg, hyaluronic acid 43 mg, vitamin C 7200 mg, vitamin B1 22 mg, vitamin B2 29 mg, vitamin B6 29 mg, vitamin V per 720 ml. B12 3.6 μg, and other added components (fructose-glucose liquid, pineapple juice, refined honey, flavoring agent and preservative), and the balance water.

<Comparative Drink A: Composition of Nuclear Protein Drink Type A> Per 720 ml, water-soluble nuclear protein 2880 mg, no collagen, no chondroitin, no hyaluronic acid, vitamin C 14400 mg, vitamin B1 44 mg, vitamin B2 58 mg, vitamin B6 58 mg, vitamin B12 7.2 μg, and other additive components (fructose-glucose liquid, pineapple juice, refined honey, flavor and preservative), and the balance water.

<Comparative Drink B: Composition of Collagen Drink Type B> Per 720 ml, water-soluble nucleoprotein-free, 160 g collagen, chondroitin 230 mg, hyaluronic acid 86 mg, vitamin C-free, vitamin B1-free, vitamin B2-free, vitamin B6 is not included, vitamin B12 is not included, and other added components (fructose glucose solution, pineapple juice, refined honey, flavor and preservative) and the balance water.

<Evaluation Test> A. Evaluation test method The difference in effect between the present healthy drink and comparative drinks A and B when tasted for a certain period of time was evaluated by a questionnaire method. FIG. 1 shows a questionnaire form for the present health drink (natural DN collagen), FIG. 2 shows a questionnaire form for comparative drink A (nuclear protein drink Type A), and FIG. 3 shows a questionnaire form for comparative drink B (collagen drink Type B). .

B. Evaluation Test Results The evaluation test results (questionnaire results) regarding the present health drink, comparative drink A and comparative drink B are shown in FIGS. 4 to 15. Each figure shows the result of drinking 60 ml per day for 30 days. That is, regarding the health drink, the results of the questionnaire for smokers are shown in FIG.
Figure 5 shows the results of the questionnaire for those who do not smoke.
FIG. 6 shows the questionnaire results for people who drink alcohol, and FIG. 7 shows the questionnaire results for people who do not drink alcohol. Similarly, regarding the comparative drink A, the result of a questionnaire for a person who smokes is shown in FIG. 8, the result of a questionnaire for a person who does not smoke is shown in FIG. 9, the result of a questionnaire for a person who drinks is shown in FIG. The results of the questionnaire for people are shown in FIG. Further, regarding the comparative drink B, the results of the questionnaire for the person who smokes are shown in FIG. 12, the results of the questionnaire for the person who does not smoke are shown in FIG. 13, the results of the questionnaire for the person who drinks are shown in FIG. 14, and the person who does not drink The result of the questionnaire for each is shown in FIG.

C. Summary of Evaluation Test Results Comparing the questionnaire results for this health drink (FIGS. 4 to 7) with the questionnaire results for comparative drink A (FIGS. 8 to 11) and the questionnaire results for comparative drink B (FIGS. 12 to 15) In this health drink, items about changes in physical symptoms (series 1
Or more than 21 items), majority (50% or more)
People say that it is effective, some 60% to 8
There are even items that many people say 0% or more than 80% are effective. On the other hand, comparative drink A
And in comparative drink B, the majority (50% or more)
There are only a few items that those who answered that are effective. From this, the water-soluble nucleoprotein-containing health drink of the present invention containing water-soluble nucleoprotein and other active ingredients that are made water-soluble by lowering the molecular weight of nucleoprotein in fish algae, respectively, is A comparative drink A and a comparative drink B, in which a synergistic effect of each active ingredient is developed and which does not contain a part of the active ingredient of the present health drink or which contains the active ingredient in an amount outside the range of the active ingredient of the present health drink. Compared to maintaining human health
It is clear that it has a better effect in promoting.

[0042]

The water-soluble nucleoprotein-containing health drink of the present invention is a drink in which the water-soluble nucleoprotein made water-soluble by lowering the molecular weight of the nucleoprotein in the agar of fish is mainly composed of collagen together with other active ingredients. Since this is an appropriate amount, this health drink is regularly added at a fixed amount (for example, 20 ml ~ daily).
Ingestion (60 ml) effectively digests and absorbs nucleic acids and arginine-based proteins, reduces oxidative damage of genes, and is very useful for maintaining and improving the health of healthy drinkers. . In addition, this health drink contains each of the properly selected active ingredients in an optimal mixing ratio, and therefore, a synergistic effect of each active ingredient is developed, and therefore, this health drink is regularly mixed. By ingesting a large amount, even for unhealthy people (for example, sick people and sick people) and people after illness, it is possible to perform digestive absorption with less burden on the body and recover the physical strength of healthy people, Further, it can reduce the oxidative damage of genes, and is therefore effective for prompt recovery of physical strength, improvement of disease symptoms and physical condition, and prevention of aging.

[Brief description of drawings]

FIG. 1 is a view showing a questionnaire form of the present health drink (natural DN collagen).

FIG. 2 is a comparative drink A (nuclear protein drink Type
It is a figure which shows the questionnaire form of A).

FIG. 3 shows comparative drink B (collagen drink
It is a figure which shows the questionnaire sheet of Type B).

[Fig. 4] Fig. 4 is a diagram showing the results of a questionnaire for people who smoke cigarettes regarding the present health drink.

[Fig. 5] Fig. 5 is a view showing a result of a questionnaire for a person who does not smoke, regarding the present health drink.

[Fig. 6] Fig. 6 is a diagram showing the results of a questionnaire for people who drink alcohol about the present health drink.

[Fig. 7] Fig. 7 is a diagram showing a result of a questionnaire for a person who does not drink alcohol about the present health drink.

FIG. 8 is a diagram showing a result of a questionnaire for a person who smokes a cigarette, for the comparative drink A.

FIG. 9 is a view showing a result of a questionnaire regarding a person who does not smoke with respect to the comparative drink A.

FIG. 10 is a diagram showing a result of a questionnaire for a drinker regarding comparative drink A.

FIG. 11 is a diagram showing a result of a questionnaire for a person who does not drink alcohol about the comparative drink A.

[Fig. 12] Fig. 12 is a view showing a result of a questionnaire for a person who smokes a cigarette, regarding Comparative Drink B.

[Fig. 13] Fig. 13 is a diagram showing a result of a questionnaire for a person who does not smoke, regarding Comparative Drink B.

FIG. 14 is a diagram showing a result of a questionnaire with respect to a drinker regarding comparative drink B.

FIG. 15 is a diagram showing a result of a questionnaire for a person who does not drink alcohol about the comparative drink B.

Claims (5)

[Claims] 1. A health drink, wherein, per 720 ml of the health drink, water-soluble nuclear protein 700 mg to 20000 mg, collagen 40 g to 150 g, chondroitin 50 mg to 200 mg, hyaluronic acid 20 mg to 80 mg, vitamin C 500 mg to 14000 mg, vitamin B12 2 mg. -40 mg, Vitamin B2 3 mg-50 mg, Vitamin B6 3 mg-50 mg, Vitamin B12 0.3 μg-7.0 μg and other additive components and the balance water, and the water-soluble nucleoprotein reduces nuclear protein in fish algae. A health drink containing a water-soluble nucleoprotein, which is a water-soluble nucleoprotein that has been made water-soluble by molecularization. 2. A health drink, wherein 720 ml of the health drink contains 1000 mg to 15000 mg of water-soluble nuclear protein, 80 g to 120 g of collagen, 80 mg to 150 mg of chondroitin, 30 mg to 60 mg of hyaluronic acid, 600 mg to 10000 mg of vitamin C, and 10 mg of vitamin B1. -30 mg, Vitamin B2 15 mg-40 mg, Vitamin B6 15 mg-40 mg, Vitamin B12 1.5 μg-5.0 μg and other additive components and the balance water, wherein the water-soluble nucleoprotein reduces nucleoprotein in fish algae. A health drink containing a water-soluble nucleoprotein, which is a water-soluble nucleoprotein that has been made water-soluble by molecularization. 3. The water-soluble nucleoprotein according to claim 1 or 2, wherein the water-soluble nucleoprotein is made to be water-soluble by reducing the molecular weight of the nucleoprotein in salmon, trout, or herring roe. A healthy drink containing nuclear protein. 4. The water-soluble nucleoprotein contains 20% by mass or more of a low molecular weight oligonucleotide / nucleoside and oligopeptide obtained by treating the nucleoprotein with a nuclease and a protease. The health drink containing the water-soluble nuclear protein according to any one of 3 above. 5. An active ingredient other than the active ingredient of the health drink according to claim 1 or 2, which comprises vitamins, minerals, amino acids, peptides, saccharides, water-soluble plant fibers,
3. The water-soluble nuclear protein-containing health drink according to claim 1 or 2, further comprising at least one selected from fruit or vegetable juice, honey, flavoring agents, preservatives and coloring agents.