JP2003212786A - Skin care medicament with bamboo extract component as active ingredient - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antiallergic agent, skin care preparation, dermatitis inhibitor and bath preparation, effective for various itches felt at the skin, serving as natural matter-derived antipruritics, and useful for preventing and treating atopic dermatitis and allergic symptoms. <P>SOLUTION: The skin care medicament, dermatitis inhibitor and bath preparation each containing bamboo extract component are provided. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a skin external preparation containing a bamboo extract as an active ingredient.

[0002]

2. Description of the Related Art Itching caused by the skin on the surface of the body when a person maintains an organism and carries out activities is a sensation or itching sensation caused by a weak stimulus on the skin or mucous membrane. . In recent years, the onset of atopic dermatitis has dramatically increased due to changes in the living environment. It is said that environmental factors greatly affect the onset of atopic dermatitis. Environmental factors are divided into non-allergic factors and allergic factors. Non-allergic factors include dryness of the skin, sweating, pressurization / loading of the skin, and scratching of the skin. The allergic factors are due to inhalation of food allergens, contact with house pets, pollen, metals and the like. In the case of non-allergic factors, contact with the factors causes itching, scratching the skin against rough itching destroys skin tissue, impairs the protective function of the skin, and causes skin hypersensitivity and pathogenic bacteria. Infectious symptoms are triggered. The allergic condition is caused by the production of immunoglobulin E (IgE) by an antigen-antibody reaction that occurs in the body, resulting in stimulation of the cell membrane of mast cells and release of active substances such as histamine, leukotriene and serotonin. These released substances have the action of promoting vascular permeability and the action of contracting smooth muscles, so they cause leukocytes and proteins to leak from blood vessels and cause inflammation to cause bronchoconstriction, causing asthma. . Further, when an allergen enters the body, T cells are sensitized, and when the allergen is contacted again, an inflammatory factor called lymphokine is released to cause a reaction. For such symptoms,
Research is being conducted to find effective factors for prevention and improvement. And, as for the antiallergic component, one that utilizes the stability of the mast cell membrane of borneol (Japanese Patent Laid-Open No. 6-111).
No. 713), those utilizing the inflammation-suppressing action of actinomycete culture (Japanese Patent Laid-Open No. 5-25053) and the like are known. Further, it is known that the intake of sesame oil has an effect of suppressing the production of leukotriene which causes an allergic reaction (Prostaglandin,
Vol. 36, No. 3, 1988). Further, there is known one that utilizes the antiallergic property of oils and fats containing docosahexaenoic acid (DHA) or linolenic acid (JP-A-2-29081). Although these naturally occurring anti-allergic agents have few side effects, their effects are not sufficient for the prevention and treatment of anti-allergic symptoms. Since many active substances represented by histamine are affected by the release from mast cells and basophils, in order to reduce the above-mentioned anti-allergic symptoms, histamine receptor inhibitors are effective. It is considered that antihistamines such as chloralphenylamine and diphenhydramine, and adrenocortical hormones are used, but when these drugs are used, side effects are a problem. For these reasons, the development of drugs with few side effects is underway, and the development of substances derived from natural products is under way. Then, an antiallergic agent such as rhinitis containing a natural bamboo extract as an active ingredient, and an orally administered agent in which a fatty acid is mixed with the antiallergic agent (Japanese Patent Application Laid-Open No. H9-96952
No. 278662) and an antioxidant using a bamboo extract together with an antioxidant (Japanese Patent Laid-Open No. 1-213389), these are exclusively orally administered and also prevent itching. It is not intended to do or improve directly. When it is intended to prevent or improve itching, among these, those used as external preparations such as ointments can stop itching by directly and effectively acting on the surface of the skin, side effects such as drowsiness. Since it is relatively small and can be used by being added together with a product for use, and it is convenient, there is no effective drug for which external drugs are being developed. As this type of natural product, a petal extract of the sunflower genus of the Asteraceae family is used (Japanese Patent Laid-Open No. 2000-351723), an extract of Japanese red pine (Japanese Patent Laid-Open No. 2000-26307), and an extract of Tate Lee (Japanese Patent Laid-Open No. 11-1999). 255566), tocopherol extract (JP 2001-163764).
No.), using chitosan (Japanese Patent Laid-Open No. 2001-5533)
No. 5) has been developed.

[0003]

The problem to be solved by the present invention is to apply a skin external preparation for preventing and improving itching derived from natural products and a skin external preparation and bath preparation for preventing and improving dermatitis due to atopic dermatitis and allergic symptoms. Is to provide.

[0004]

Means for Solving the Problems The inventors of the present invention have conducted extensive studies to solve the above problems, and a composition containing components contained in bamboos was subjected to a histamine release inhibition test and a leukotriene secretion inhibition test, It has high activity and is found to act effectively on allergic symptoms such as allergic dermatitis and atopic dermatitis.In addition, allergic dermatitis, atopic dermatitis and allergic disease in which histamine is actually involved. When used for symptoms, insect bites, and pruritus dermatitis due to environmental factors, a remarkable effect was observed in a short time on a wide range of pruritus symptoms, and it was prevented and improved as an internal or external drug for various pruritus. It is effective in preventing itchiness when used as a functional food, and when used as a bath preparation, it is used in functional foods to prevent and improve itchiness. And we found that it is effective,
The present invention has been completed.

That is, according to the present invention, the following inventions are provided. (1) A skin external preparation containing a bamboo extract component. (2) A dermatitis inhibitor comprising a bamboo extract component. (3) A bath agent containing a bamboo extract component.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION As the raw material bamboo used in the present invention, bamboos of the genus Gypsophila, the genus Siphonia, the genus Madha, the genus Sudasa, the genus Sasamorphua, the genus Pleurotus, the genus Astragalus, etc. are used. Among them, Mensobu and Hachiku of the genus Bamboo are preferably used. Chips obtained by cutting these bamboos are used as the raw material of the present invention. As the pulverized product, a pulverized product is used in consideration of the extraction operation.

In the present invention, an extract is used by treating a crushed bamboo product with water or an organic solvent.

The composition obtained as an extract with water after removing the insoluble matter by contacting the pulverized bamboo product with an excessive amount of water can be used as a skin external preparation. The amount of water is such that the crushed bamboo product can be sufficiently immersed, and the amount can be adjusted as appropriate as long as it can be taken out as an aqueous solution. Usually, hot water can be used for refluxing. It can also be performed under pressure. The extract generally obtained is in a slightly yellowish, transparent state. The obtained extract is concentrated and taken out as a powder.

A substance obtained by contacting a pulverized product of bamboo with an organic solvent to obtain an extract with the organic solvent can be used as a drug. Examples of the organic solvent include alcohols such as ethanol, methanol and isopropanol, polyhydric alcohols such as glycerin, ethylene glycol, propylene glycol and 1,3-butylene glycol or derivatives thereof,
Examples thereof include ketones such as acetone and methyl ethyl ketone, esters such as ethyl acetate and isopropyl acetate, and ethers such as ethyl ether and isopropyl ether. In addition, petroleum ether, n-hexane, n
-Aliphatic hydrocarbons such as pentane and the like can also be used. Among these, ethanol, 1,3-butylene glycol, propylene glycol and the like are used. These may be used as a mixed solvent including water. In any case, after extracting with a solvent, it is possible to further bring out a specific component by contacting with another solvent, but a solvent containing ethanol or 1,3-butylene glycol without performing such an operation. Can be used by directly applying it to the affected area or applying it in a state in which it is soaked in gauze or the like. 0.001 to 20% by weight of crushed bamboo as 50% 1,3-butylene glycol extract
It is generally used as a medium-level one. Among these, anti-inflammatory agents and anti-allergic disease agents such as azulene, diphenhydramine hydrochloride, dl-α-tocopherol and derivatives thereof can also be used. Further, surfactants such as natural polymers such as polysaccharides, starch, water-soluble polymers, anions, cations and nonions can also be used.

When used as a bathing agent, a powdered pulverized bamboo product mixed with sodium hydrogencarbonate, anhydrous sodium sulfate, borax, fragrance, stabilizer, etc. can be dissolved in water and used. . Further, it can also be used in the form of a liquid obtained by mixing liquid Benica miquillis with jojoba oil, perfume oil, pigment, humectant, and surfactant.

The effect of the extract of the crushed bamboo product of the present invention can be confirmed by carrying out a test for histamine release inhibition and leukotriene secretion inhibition. Moreover, it can be performed by confirming the effect of the result actually used.

[0012]

EXAMPLES The contents of the present invention will be described below with reference to examples. Example 1 Production of Ethanol Extraction Solution of Crushed Bamboo Material 10 g of Moso bamboo crushed material was added to 500 ml of ethanol, and then heated under reflux for 2 hours. Insoluble matter is filtered, and 10
The filtrate was concentrated to 0 ml to obtain an ethanol extract.

Example 2 Production of hot water extract of crushed bamboo product 10 g of Moso bamboo crushed product was dissolved in 500 ml of water, heated and heated under reflux conditions. The heat treatment was performed for 5 hours. To the water extract of the pulverized bamboo product obtained by removing the insoluble matter from the obtained product, water was similarly added twice to repeat the extraction operation to obtain an extract. Next, a concentration operation was performed. As a result, powdery bamboo water extract 26 remaining after concentration
7.6 mg was obtained. In the following examples, a solution prepared by dissolving this in water in the required amount is used.

Example 3 Histamine Release Inhibition Test The histamine release inhibiting effect of the aqueous extract of bamboo chips was evaluated using an antiallergic action test method using histamine released from rat-derived basophil cells (RBL-2H3) as an index. did. 2.5x1 RBL-2H3 on a 24-well plate
0 ⁵ cells were seeded such that the cells / well, cultured for 24 hours (5% carbon dioxide, 37 °), Phosphate buffer sal
After washing once with ine (PBS), 180 μL of bamboo chip extract / 20 mM Hepes-DMEM at each concentration was added, and the mixture was incubated at 37 ° C. for 5 minutes with carbon dioxide 5%, and then,
20 stimulants A23187 (calcium ionophore)
After adding μL and culturing for 30 minutes, 200 μL of the supernatant was taken out. In addition, 20 mM Hepes-DMEM (Mediu
m) and surfactant 0.05% Triton X-10
Using 0 (the total histamine content in the cell is measured by lysing the cell wall), the supernatants obtained by stimulation in the same manner as above were designated as Negative Control and Positive Control, respectively. The histamine content of the supernatant removed in this way was
amine enzyme-linked immunoadsorbent assay (ELI
SA) Kit (manufactured by ICN Pharmaceuticals) was used for quantification. The histamine release rate and the histamine release rate with respect to the control were calculated using Equations 1 and 2. The histamine release rate at each concentration relative to the control is shown in FIG. Histamine release rate (%) = ((A−B) / (C−B)) × 100 (Equation 1) * A is A23 in cells treated with the bamboo chip extract at each concentration.
Histamine content in the supernatant stimulated with 187 * B is histamine content in the supernatant by Negative Control * C is histamine content in the supernatant by Positive Control Histamine release rate (%) = (D / E) x 100 ... -(Formula 2) * D is the histamine release rate (%) of the bamboo chip extract at each concentration * E is the A23817 stimulation only (untreated with bamboo chip extract) histamine release rate (%) * The control is A23187 stimulation The histamine release rate (%) in the supernatant of only (untreated with bamboo chip extract) was 0.9603 mg / ml, which is the result of the blank, and was significantly reduced at 0.625 mg / ml. Then 1.25mg / ml, 1.25mg / m
The value gradually decreases after l. It can be seen that the histamine release inhibitory effect is sufficient.

Example 4 Leukotriene Secretion Inhibition Test The leukotriene secretion inhibitory effect of bamboo chip water extract was evaluated using an antiallergic activity test method using leukotriene secreted from rat-derived basophil cells (RBL-2H3) as an index. did. RBL-2H3 was seeded on a 24-well plate at 2.5 × 10 ⁵ cells / well, and 2
After culturing for 4 hours (carbon dioxide 5%, 37 degrees), Phosphate
After washing once with buffer saline (PBS), each concentration of bamboo chip extract / 20 mM Hepes-DMEM 1
After adding 80 μL and culturing for 30 minutes at 37 ° C. and 5% carbon dioxide, 20 μL of antigen was added and stimulated for 30 minutes, and 200 μL of the supernatant was taken out. The amount of leukotriene secreted in the supernatant taken out in this manner was Leukotriene C ₄
Enzyme immunoassay (EIA) Kit (Cayman Chem
ical, Ann Arbor, MI). The amount of leukotriene secreted was determined based on the standard solution of leukotriene (0-10
It was calculated using a calibration curve based on (00 pg / mL). The leukotriene secretion inhibitory effect by each concentration is shown in FIG. Results For the blank results 2521 pg / ml,
A decrease was seen at 0.625 mg / ml, then 1.
It decreased sharply for 25 and 2.5 mg. From this, the effect of suppressing the leukotriene secretion of the water extract of bamboo could be sufficiently confirmed.

Example 5 An aqueous extract of bamboo was dissolved in purified water to a concentration of 0.005%, which was applied to the affected area at any time when itching was observed, and the results shown in Table 1 were obtained for the improvement status of itching symptoms. . During the period of use of this drug, the use of other antiallergic agents such as antihistamines was discontinued. Cases 1 to 4 to which the external preparation for skin according to the present invention is applied include pruritus dermatitis, atopic dermatitis,
It is related to athlete's foot and heat rash. The results show that this drug is extremely effective for itching due to all these symptoms, and no case was found to have an antipruritic effect or no worsening of symptoms due to application. Table 1 shows the results organized into a table.

[0017]

[Table 1]

Results As is clear from the above description of the table, it can be said that many of them were significantly effective, and some of them were effective, and that they were significantly effective as a whole. There was no ineffectiveness or deterioration. It can be understood that it is effective for itching due to various causes.

An example of prescription is shown below. The present invention is
Needless to say, it is not limited to this. Lotion-like pruritus preventive / alleviator Ethanol 15.00 (wt%) Hydroxyethyl cellulose 0.10 Methyl paraoxybenzoate 0.10 Bamboo water extract 0.20 Purified water 84.60

[0020] Milky pruritus preventive / relief agent     Stearic acid 0.2 (wt%)     Cetanol 1.5     Vaseline 3.0     Liquid Paraffin 7.0     Polyoxyethylene (10EO)         Monooleate 1.5     Tocopherol acetate 0.2     Glycerin 5.0     Methyl paraoxybenzoate 0.1     Triethanolamine 0.1     Bamboo water extract 0.4     Purified water 81.0

[0021] Gel itching preventive and reducer     Dipropylene glycol 10.0 (wt%)     Carboxy vinyl polymer 0.5     Potassium hydroxide 0.1     Methyl paraoxybenzoate 0.1     Bamboo water extract 0.5     Purified water 88.8

[0022] Creamy itching and anti-allergic agent     Beeswax 6.0 (wt%)     Cetanol 5.0     Reduced lanolin 8.0     Squalene 27.5     Glycer fatty acid ester 4.00     Lipophilic glycer monostearate 2.0     Polyoxyethylene (20EO) sorbitan         Monolauryl ester 5.0     Propylene glycol 5.0     Paraoxy benzoyl methyl 0.1     Bamboo water extract 0.5     Purified water 36.9

[0023] Powdery pruritus reducing agent     Magnesium silicate magnesium 95.3 (wt%)     Carboxymethyl cellulose calcium 4.5     Bamboo extract dry powder 0.2

[0024] Bath agent     Sodium hydrogen carbonate 63.0 (wt%)     Anhydrous sodium sulfate 30.0     Borax 2.0     Bamboo extract dry powder 5.0

[0025] Bath agent     Refined jojoba oil 5.0 (wt%)     Polyoxyethylene sorbitan monolaurate 20.0     Glycerin monolaurate 5.0     Fluid Paraffin 2.0     Lauric acid diethanolamide 2.0     Bamboo extract 3.0     Purified water 67.0

[0026]

The external preparation for skin, the dermatitis inhibitor, and the bath preparation containing the extract of the pulverized bamboo product according to the present invention are allergic dermatitis, atopic dermatitis, allergic symptoms, and insects. And is useful as a prophylactic and therapeutic drug for pruritus skin.

[Brief description of drawings]

FIG. 1 is a view showing the results of a histamine release inhibition test of a water extract of ground bamboo products.

FIG. 2 is a view showing the results of a leukotriene secretion inhibition test of a water extract of a pulverized product of bamboo.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Minoru Ishikawa             4-8-24, Kudan-minami 4-chome, Chiyoda-ku, Tokyo             Nihon University (72) Inventor Seishiro Mochizuki             4-8-24, Kudan-minami 4-chome, Chiyoda-ku, Tokyo             Nihon University (72) Inventor Hirotaka Kishida             4-8-24, Kudan-minami 4-chome, Chiyoda-ku, Tokyo             Nihon University F-term (reference) 4C083 AA111 AA112 AA122 AB152                       AB312 AB352 AC022 AC422                       AC442 AC642 CC25 DD17                       DD31 EE12 EE42                 4C088 AB76 AC05 AC06 BA08 CA03                       MA63 NA14 ZA89 ZB11 ZB13

Claims (3)

[Claims] 1. A skin external preparation containing a bamboo extract component. 2. A dermatitis inhibitor comprising a bamboo extract component. 3. A bath agent containing a bamboo extract component.