JP2003206483A - Antioxidant and skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant and a skin care preparation having an excellent antioxidizing ability and bleaching actions. <P>SOLUTION: This antioxidant comprises an extract from a herb generically called Lagotis brevituba Maxim., Lagotis clarkei Hook. f., Lagotis maerosiphon Tsoong et. H. P. Yang and Lagotis ramalana Batalin or Cortex Berberidis. The skin care preparation contains the extract. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antioxidant and an external preparation for skin, and more specifically, to pharmaceuticals, quasi drugs, cosmetics,
The present invention relates to an antioxidant having excellent safety and antioxidant ability that can be added to foods and the like, and a skin external preparation having excellent antioxidant ability and whitening effect.

[0002]

2. Description of the Related Art Conventionally, the problems to be solved by the invention
Synthetic antioxidants such as BHT and BHA, various antioxidants such as α-tocopherol and ascorbic acid, and various plant extracts are used for cosmetics, pharmaceuticals, foods and the like. However, there were some concerns regarding safety, and the effects were still unsatisfactory.

In view of such a situation, the inventors of the present invention have made earnest researches to improve the difficulties of the prior art, and as a result,
The present inventors have found that the extract of rabbit ear grass or small cucumber has an excellent antioxidant effect and a whitening effect, and have completed the present invention.

That is, an object of the present invention is to provide an antioxidant and a skin external preparation which are highly safe and have excellent antioxidant ability.

[0005]

DISCLOSURE OF THE INVENTION The present invention provides an antioxidant characterized by comprising an extract of Rabbit ears grass or small cucumber, and a skin external preparation containing the extract. It achieves the purpose.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The rabbit ear grass used in the present invention is
Also referred to as rabbit ear grass, it is a short-tube rabbit ear grass (Lagotis br.
evituba Maxim. ), Large calyx ear grass (Lag
otis clearei Hook. f. ), Lagotis maerosiphon T
soong et. H. P. (Yang) and Enzo rabbit (Lagotis ramalana Batali)
n) is a general term and can be used alone or in combination, and Konohide has the scientific name Cortex B.
erberidis, Gansu Konoha (Berneri)
S Kansuensis Schneid. ), And has been used as a crude drug in Tibet. Rabbit eargrass has been shown to have antilipidemic effects, and small crust is effective for eye strain.

The extraction solvent for obtaining the extract of rabbit ear grass or small cucumber is, for example, lower alcohol such as water, ethanol, propanol, butanol, 1,3-butylene glycol, propylene glycol, glycerin, dipropylene glycol. Examples thereof include polyhydric alcohols such as, and mixtures thereof. Of these, water alone, a mixed solution of water and ethanol, and a mixed solution of water and 1,3-butylene glycol are preferable, and the ratio of ethanol or 1,3-butylene glycol and water is 1: 1 to 1:99 ( (Volume ratio) is more preferable. The extraction may be performed at low temperature or at room temperature while heating. From the viewpoint of evaluation, low-pitched extraction is preferable. The obtained extract may be purified and filtered as appropriate, or may be concentrated and dried by a conventional method before use.

[0008] The amount of the rabbit eargrass or small cucumber extract compounded in the external preparation for skin varies widely depending on the purpose, the site to be treated, etc. and cannot be specified unconditionally. Generally from 0.001 to 10 mass% based on the total amount
(Hereinafter, simply referred to as “%”) is preferable, and 0.01 to 3% is more preferable.

The antioxidant according to the present invention comprises the above extract,
It can be used after being dissolved or dispersed in fats and oils, solvents and the like.
Further, it is possible to make granules or powders by appropriately using excipients or the like generally used for pharmaceuticals, foods, etc., and to make liquids, emulsions, creams, pastes, etc. by using an appropriate solvent or the like. Further, it is more preferable to contain a synergistic agent such as ascorbic acid, citric acid or succinic acid, which is known to exert a synergistic effect when used in combination with an antioxidant if necessary.

The antioxidant of the present invention is used not only in cosmetics such as lipsticks, emulsions and creams, pharmaceuticals, quasi drugs, but also fish oil, lard, soybean oil, linseed oil, corn oil, cottonseed oil, palm oil. It can be widely used for oils and fats derived from animals and plants such as, and foods such as butter, margarine, mayonnaise, ham, potato chips, fried rice crackers, fish meat, etc. without impairing the taste, aroma, feel and the like.

When the extract according to the present invention is used in cosmetics, it can be blended in various cosmetics such as skin cosmetics, makeup cosmetics, hair cosmetics and bath salts, and its dosage forms are lotions, creams, ointments, Various types such as emulsions, paps, gels, powders and granules can be used. In particular, the extract of the present invention has an antioxidant effect, so that it not only prevents the oxidation of the contents but also suppresses the generation of peroxide due to ultraviolet rays and the like, and the effect of preventing skin aging phenomena including wrinkles. In addition to being obtained, it also has a melanin synthesis inhibitory action, so that it is possible to exert a whitening effect on spots, freckles, and dullness. Therefore, the form applied directly to the skin is preferable, and the skin cosmetic is particularly preferable. still. In the present invention, the external preparation for skin includes all those applied to the skin on the body including the scalp, for example, cosmetics, pharmaceuticals,
Bath salts are included without distinction between quasi-drugs and the like.

[0012]

EXAMPLES Examples will be described below. Moreover,% shown in an example means the mass%. The present invention is not limited to the examples below. A freeze-dried solid substance was used as each extract. As the rabbit ear grass, short-cylinder rabbit ear grass was used.

Test Example 1 (Antioxidant test) 0.05 mol / L sodium carbonate buffer solution (pH 10.
2) 2.4 mL, 3 mmol / L xanthine 0.1 m
L, 3 mmol / L EDTA 0.1 mL, 0.15%
To the composition of 0.1 mL of bovine serum albumin and 0.7 mL of 0.75 mmol / L nitroblue tetrazolium, 0.1 mL of water or 50% ethanol solution having a concentration of 1.5 mg / mL of the sample was added and heated at 25 ° C. for 10 minutes. To do. To this, 0.1 mL of 250-fold diluted xanthine oxidase (manufactured by Sigma) derived from buttermilk is added and reacted at 25 ° C. for 20 minutes. 6 mL / L cupric chloride 0.1 mL was added, the reaction was stopped, and the absorbance was measured at a wavelength of 560 nm using a spectrophotometer. Instead of the sample solution, 0.1 mL of water or 50% ethanol was added, and the absorbance was set to 100 to determine the inhibition rate (%).

As a result, as shown in Table 1, it was confirmed that the extract used in the present invention has an excellent antioxidant ability.

[0015] Table 1 Antioxidant test results   Sample concentration suppression rate (%) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−   Rabbit eargrass water extract 0.005% 91.7       (Aqueous solution)   Rabbit eargrass 50% ethanol extract 0.005% 90.4       (50% ethanol solution)   Small scales water extract 0.005% 73.1       (Aqueous solution)   Small crust 50% ethanol extract 0.005% 68.6       (50% ethanol solution)

[0016] Test Example 2 (melanin synthesis inhibition test) (1) melanogenesis inhibition test B16 melanoma cells at 2 × 10 ⁴ cells / well 12
24 hours after plating on a well plate, 10μ of each sample compound
Theophylline (T containing g / mL or 30 μg / mL)
The medium was replaced with a medium containing heophylline). After culturing for 72 hours, cells were treated with 10% TCA and ethanol / diethyl ether (= 1/1). continue,
The OD475 value after dissolution in a 1 mol / L sodium hydroxide aqueous solution containing 10% dimethyl sulfoxide was determined and defined as the amount of melanin. After that, the number of cells was measured to determine the inhibition rate (%) of melanin production per cell.

As a result, as shown in Table 2, it was confirmed that the extract used in the present invention had an excellent melanin synthesis inhibitory effect.

[0018] Table 2 Melanin synthesis inhibition test results   Sample concentration Inhibition rate −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−   Rabbit ear grass water extract 10 μg / ml 20.9%   Rabbit ear grass 50% ethanol extract 10 μg / ml 84.6%   Small cucumber water extract 10 μg / ml 55.2%                                   30 μg / ml 73.3%   Small crust 50% ethanol extract 10 μg / ml 50.6%                                   30 μg / ml 76.3%

From the above results, it was found that the extract of the present invention has an excellent antioxidative ability and further an inhibitory effect on melanin synthesis.

Examples 1 to 8 (skin cream) In the composition of Table 3, the extract was blended as shown in Table 4 to prepare a skin cream according to the following preparation method.

[0021]   (Table 3) Blending ingredients Blending amount (%) (A) Oil phase   Stearic acid 7.0   Oleic acid 3.0   Stearyl alcohol 4.0   Stearic acid monoglycerin ester 8.0   Extract (listed in Table 4)   Ethyl paraben 0.1   Butylparaben 0.1   Propylparaben 0.1 (B) Water phase   Propylene glycol 8.0   Glycerin 2.0   Potassium hydroxide 0.4   Disodium edetate 0.05   Purified water Remaining amount based on 100

[0022] (Table 4)                               Example 1 Example 2 Example 3 Example 4 Rabbit eargrass water extract 0.5% − − − Rabbit eargrass water extract − 0.1% − − Rabbit eargrass 50% ethanol extract − − 0.5% − Rabbit ear grass 50% ethanol extract − − − 0.1%                               Example 5 Example 6 Example 7 Example 8 Small cucumber water extract 0.5% − − − Small cucumber water extract − 0.1% − − 50% ethanol extract of small cucumber − − 0.5% − Small crust 50% ethanol extract − − − 0.1%

(Preparation Method) The oil phase portion of A and the water phase portion of B were heated to 70 ° C. to completely dissolve them. Next, Phase B was added to Phase A and emulsified with an emulsifying machine. The emulsion was cooled and filled in a container to obtain a skin cream according to the present invention.

It was confirmed that the skin creams of Examples 1 to 8 according to the present invention showed no skin irritation and were excellent in stability over time.

Examples 9 to 20 (skin lotion) In the composition of Table 5, the extract was blended as shown in Table 6, and the skin lotion was prepared according to the following preparation method.

[0026] (Table 5) Blending ingredients Blending amount (%) (A)   Ethanol 10.0   Polyoxyethylene (6) sorbitan monolaurate 5.0   Fragrance 0.05 (B)   Extract (listed in Table 6) (C)   Glycerin 5.0   Xanthan gum 0.1   Hydroxyethyl cellulose 0.1   Purified water Remaining amount based on 100

[0027] (Table 6)                                 Example 9 Example 10 Example 11 Rabbit ear grass water extract 0.1% − − Rabbit ear grass water extract-1.0%- Rabbit eargrass water extract − − 3.0%                                 Example 12 Example 13 Example 14 Rabbit eargrass 50% ethanol extract 0.1% − − Rabbit ear grass 50% ethanol extract-1.0%- Rabbit ear grass 50% ethanol extract --3.0%                                 Example 15 Example 16 Example 17 Small cucumber water extract 0.1% − − Small cucumber water extract-1.0%- Small cucumber water extract − − 3.0%                                 Example 18 Example 19 Example 20 Small cucumber skin 50% ethanol extract 0.1% − − Small crust 50% ethanol extract-1.0%- 50% ethanol extract --- 3.0%

(Preparation Method) After the component B was uniformly dissolved in the component C, the components A and C were uniformly mixed and stirred, dispersed, and then filled in a container to obtain a skin lotion according to the present invention.

It was confirmed that the skin lotions according to the present invention of Examples 9 to 20 showed no skin irritation and were excellent in stability over time.

Examples 21 to 32 (lipsticks) In the composition of Table 7, the extracts were blended as shown in Table 8 to prepare lipsticks according to the following preparation method.

[0031] (Table 7) Blending ingredients Blending amount (%) (A)   Paraffin 16.0   Polybutene 10.0   Vaseline 20.0   Octyldodecanol 10.0   Liquid paraffin Remaining amount based on 100 (B)   Red No. 201 1.0   Red No. 202 0.5   Yellow No. 4 aluminum rake 0.3   Black iron oxide 0.1 (C)   Extract (listed in Table 8)

[0032] (Table 8)                                   Example 21 Example 22 Example 23   Rabbit ear grass water extract 0.01% − −   Rabbit ear grass water extract-0.3%-   Rabbit eargrass water extract − − 3.0%                                   Example 24 Example 25 Example 26   Rabbit ear grass 50% ethanol extract 0.01% − −   Rabbit ear grass 50% ethanol extract-0.3%-   Rabbit ear grass 50% ethanol extract --3.0%                                   Example 27 Example 28 Example 29   Small cucumber water extract 0.01% − −   Small cucumber water extract-0.3%-   Small cucumber water extract − − 3.0%                                      Example 30 Example 31 Example 32   Small crust 50% ethanol extract 0.01% − −   Small crust 50% ethanol extract-0.3%-   50% ethanol extract --- 3.0%

(Preparation Method) Component A was uniformly dissolved at 90 ° C., and component B was added and kneaded. Redissolve at 90 ° C,
After degassing, add the C component and pour into a mold for cooling and molding.
The lipstick according to the present invention was obtained by putting it in a container.

It was confirmed that the lipsticks of Examples 21 to 32 according to the present invention showed no skin irritation and were excellent in stability over time.

[0035]

The anti-oxidant and the external skin preparation according to the present invention have excellent anti-oxidant ability and whitening effect and prevent skin aging phenomena such as spots, freckles, dullness and wrinkles. When used as a skin external preparation, it is extremely useful.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/00 A61K 7/00 X 7/027 7/027 7/48 7/48 35/78 35/78 CG A61P 17/00 A61P 17/00 39/06 39/06 (72) Inventor Sayuri Yamaguchi 5-328, Kotobuki-cho, Odawara-shi, Kanagawa F-Term (Reference) 4B021 LW03, Kanebo Corporation LW04 LW07 LW08 MC03 MK05 MQ04 4C083 AA111 AA112 AB232 AC012 AC022 AC032 AC072 AC102 AC122 AC242 AC252 AC392 AC422 AC442 AC482 AC532 AC792 AC852 AD022 AD282 AD352 BB47 CC02 CC04 CC05 CC13 DD06 DD21 DD31 EE12 EE13 AB06AB020 ZC41 4H025 BA01

Claims (2)

[Claims] 1. An antioxidant characterized by comprising an extract of rabbit eargrass or small cucumber. 2. An external preparation for skin, which comprises an extract of rabbit eargrass or small cucumber.