JP2003111587A - ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物 - Google Patents
ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物Info
- Publication number
- JP2003111587A JP2003111587A JP2002179815A JP2002179815A JP2003111587A JP 2003111587 A JP2003111587 A JP 2003111587A JP 2002179815 A JP2002179815 A JP 2002179815A JP 2002179815 A JP2002179815 A JP 2002179815A JP 2003111587 A JP2003111587 A JP 2003111587A
- Authority
- JP
- Japan
- Prior art keywords
- reporter gene
- rev
- gene construct
- tat
- reporter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 210000004027 cell Anatomy 0.000 claims abstract description 91
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 39
- 238000000034 method Methods 0.000 claims abstract description 35
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- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 0 CCN(C)C=*=C Chemical compound CCN(C)C=*=C 0.000 description 1
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- 101100321669 Fagopyrum esculentum FA02 gene Proteins 0.000 description 1
- 241000700662 Fowlpox virus Species 0.000 description 1
- 101710177291 Gag polyprotein Proteins 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
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- 102100036738 Guanine nucleotide-binding protein subunit alpha-11 Human genes 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- 101000867841 Homo sapiens Carbonic anhydrase-related protein 11 Proteins 0.000 description 1
- 101100283445 Homo sapiens GNA11 gene Proteins 0.000 description 1
- 101001075218 Homo sapiens Gastrokine-1 Proteins 0.000 description 1
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- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10130155A DE10130155B3 (de) | 2001-06-22 | 2001-06-22 | Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat |
| DE10130155.3 | 2001-06-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003111587A true JP2003111587A (ja) | 2003-04-15 |
| JP2003111587A5 JP2003111587A5 (OSRAM) | 2005-09-29 |
Family
ID=7689082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002179815A Pending JP2003111587A (ja) | 2001-06-22 | 2002-06-20 | ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7183047B2 (OSRAM) |
| EP (1) | EP1270744A1 (OSRAM) |
| JP (1) | JP2003111587A (OSRAM) |
| DE (1) | DE10130155B3 (OSRAM) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10053781B4 (de) * | 2000-10-30 | 2008-07-03 | Geneart Ag | Kernexportreportersystem |
| CA2540253A1 (en) * | 2003-09-28 | 2005-04-14 | The United States Of America, As Represented By The Secretary, Departmen T Of Health And Human Services | Hiv-dependent expression constructs and uses therefor |
| US8617816B2 (en) * | 2007-03-16 | 2013-12-31 | 454 Life Sciences, A Roche Company | System and method for detection of HIV drug resistant variants |
| US10106818B2 (en) | 2013-08-16 | 2018-10-23 | The J. David Gladstone Institutes | Dual-color HIV reporter system for the detection of latently-infected cells |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5654398A (en) * | 1993-06-03 | 1997-08-05 | The Regents Of The University Of California | Compositions and methods for inhibiting replication of human immunodeficiency virus-1 |
| WO1997009342A1 (en) * | 1995-09-08 | 1997-03-13 | Scriptgen Pharmaceuticals, Inc. | Screen for compounds with affinity for rna |
| US6225045B1 (en) * | 1996-05-13 | 2001-05-01 | Ribotargets, Ltd. | Assays for screening for inhibitors of HIV |
| US5989814A (en) * | 1997-04-01 | 1999-11-23 | Reagents Of The University Of California | Screening methods in eucaryotic cells |
| WO1999039011A1 (en) * | 1998-01-30 | 1999-08-05 | Duke University | A method of assaying for rna:protein interactions |
| US6573045B1 (en) * | 1998-06-05 | 2003-06-03 | Ribotargets, Ltd. | Methods and kits for discovery of RNA-binding compounds |
-
2001
- 2001-06-22 DE DE10130155A patent/DE10130155B3/de not_active Expired - Fee Related
-
2002
- 2002-06-20 JP JP2002179815A patent/JP2003111587A/ja active Pending
- 2002-06-20 US US10/176,010 patent/US7183047B2/en not_active Expired - Fee Related
- 2002-06-21 EP EP02013820A patent/EP1270744A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1270744A1 (en) | 2003-01-02 |
| US20030092032A1 (en) | 2003-05-15 |
| US7183047B2 (en) | 2007-02-27 |
| DE10130155B3 (de) | 2004-03-04 |
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