JP2002535016A - アズラクトン官能性担体を使用したホールセルの選択 - Google Patents
アズラクトン官能性担体を使用したホールセルの選択Info
- Publication number
- JP2002535016A JP2002535016A JP2000596375A JP2000596375A JP2002535016A JP 2002535016 A JP2002535016 A JP 2002535016A JP 2000596375 A JP2000596375 A JP 2000596375A JP 2000596375 A JP2000596375 A JP 2000596375A JP 2002535016 A JP2002535016 A JP 2002535016A
- Authority
- JP
- Japan
- Prior art keywords
- azlactone
- carrier
- biologically active
- functional
- whole cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- IEJPPSMHUUQABK-UHFFFAOYSA-N 2,4-diphenyl-4h-1,3-oxazol-5-one Chemical compound O=C1OC(C=2C=CC=CC=2)=NC1C1=CC=CC=C1 IEJPPSMHUUQABK-UHFFFAOYSA-N 0.000 title claims description 41
- 239000000969 carrier Substances 0.000 title description 26
- 238000000034 method Methods 0.000 claims abstract description 44
- 229940088623 biologically active substance Drugs 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 108090001008 Avidin Proteins 0.000 claims description 20
- 230000008878 coupling Effects 0.000 claims description 18
- 238000010168 coupling process Methods 0.000 claims description 18
- 238000005859 coupling reaction Methods 0.000 claims description 18
- 239000000427 antigen Substances 0.000 claims description 16
- 239000011324 bead Substances 0.000 claims description 16
- 102000036639 antigens Human genes 0.000 claims description 15
- 108091007433 antigens Proteins 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 14
- 239000013543 active substance Substances 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 8
- 239000000178 monomer Substances 0.000 claims description 8
- 210000005259 peripheral blood Anatomy 0.000 claims description 7
- 239000011886 peripheral blood Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 125000004432 carbon atom Chemical group C* 0.000 claims description 6
- 239000011859 microparticle Substances 0.000 claims description 6
- 238000005497 microtitration Methods 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 230000001588 bifunctional effect Effects 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 229920000578 graft copolymer Polymers 0.000 claims description 4
- 125000006413 ring segment Chemical group 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 108090001090 Lectins Proteins 0.000 claims description 3
- 102000004856 Lectins Human genes 0.000 claims description 3
- 230000027455 binding Effects 0.000 claims description 3
- 210000001185 bone marrow Anatomy 0.000 claims description 3
- 239000007943 implant Substances 0.000 claims description 3
- 239000002523 lectin Substances 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000011282 treatment Methods 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 125000000217 alkyl group Chemical group 0.000 claims description 2
- 125000005282 allenyl group Chemical group 0.000 claims description 2
- 125000003118 aryl group Chemical group 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 239000003431 cross linking reagent Substances 0.000 claims description 2
- 125000004122 cyclic group Chemical group 0.000 claims description 2
- 125000000753 cycloalkyl group Chemical group 0.000 claims description 2
- 238000012674 dispersion polymerization Methods 0.000 claims description 2
- 125000005842 heteroatom Chemical group 0.000 claims description 2
- 125000000864 peroxy group Chemical group O(O*)* 0.000 claims description 2
- 238000010558 suspension polymerization method Methods 0.000 claims description 2
- 239000011149 active material Substances 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 87
- 239000000463 material Substances 0.000 description 19
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 239000000523 sample Substances 0.000 description 11
- 230000003993 interaction Effects 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- WHNPOQXWAMXPTA-UHFFFAOYSA-N 3-methylbut-2-enamide Chemical compound CC(C)=CC(N)=O WHNPOQXWAMXPTA-UHFFFAOYSA-N 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- 239000003446 ligand Substances 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 6
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 6
- 230000009871 nonspecific binding Effects 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 210000000130 stem cell Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- -1 antibodies Proteins 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000012050 conventional carrier Substances 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000004971 Cross linker Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 210000000612 antigen-presenting cell Anatomy 0.000 description 2
- 229920005601 base polymer Polymers 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 238000003320 cell separation method Methods 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000010414 supernatant solution Substances 0.000 description 2
- 238000010557 suspension polymerization reaction Methods 0.000 description 2
- YUDNXDTXQPYKCA-YDHLFZDLSA-N (4-nitrophenyl) 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate Chemical compound C1=CC([N+](=O)[O-])=CC=C1OC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1 YUDNXDTXQPYKCA-YDHLFZDLSA-N 0.000 description 1
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 description 1
- FPBFWDHYZMVTJD-UHFFFAOYSA-N 2-methyl-2-(prop-2-enoylamino)propanoic acid Chemical compound OC(=O)C(C)(C)NC(=O)C=C FPBFWDHYZMVTJD-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102100033779 Collagen alpha-4(IV) chain Human genes 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 101000710870 Homo sapiens Collagen alpha-4(IV) chain Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000009295 crossflow filtration Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000000998 lymphohematopoietic effect Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 108010087904 neutravidin Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 238000000614 phase inversion technique Methods 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
- Y10S530/816—Attached to the carrier via a bridging agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T442/00—Fabric [woven, knitted, or nonwoven textile or cloth, etc.]
- Y10T442/20—Coated or impregnated woven, knit, or nonwoven fabric which is not [a] associated with another preformed layer or fiber layer or, [b] with respect to woven and knit, characterized, respectively, by a particular or differential weave or knit, wherein the coating or impregnation is neither a foamed material nor a free metal or alloy layer
- Y10T442/2525—Coating or impregnation functions biologically [e.g., insect repellent, antiseptic, insecticide, bactericide, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/240,829 | 1999-02-01 | ||
| US09/240,829 US6379952B1 (en) | 1999-02-01 | 1999-02-01 | Method for cell selection utilizing azlactone-functional supports |
| PCT/US1999/009497 WO2000045174A1 (en) | 1999-02-01 | 1999-04-30 | Whole cell selection utilizing azlactone-functional supports |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002535016A true JP2002535016A (ja) | 2002-10-22 |
| JP2002535016A5 JP2002535016A5 (https=) | 2006-06-29 |
Family
ID=22908111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000596375A Pending JP2002535016A (ja) | 1999-02-01 | 1999-04-30 | アズラクトン官能性担体を使用したホールセルの選択 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6379952B1 (https=) |
| EP (1) | EP1149289B1 (https=) |
| JP (1) | JP2002535016A (https=) |
| CN (1) | CN1141582C (https=) |
| AU (1) | AU3875299A (https=) |
| DE (1) | DE69920972T2 (https=) |
| WO (1) | WO2000045174A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6379952B1 (en) * | 1999-02-01 | 2002-04-30 | 3M Innovative Properties Company | Method for cell selection utilizing azlactone-functional supports |
| US20030040125A1 (en) * | 2001-08-21 | 2003-02-27 | 3M Innovative Properties Company | Methods for performing immunological assays |
| US7883720B2 (en) | 2003-07-09 | 2011-02-08 | Wisconsin Alumni Research Foundation | Charge-dynamic polymers and delivery of anionic compounds |
| WO2006044532A1 (en) * | 2004-10-15 | 2006-04-27 | 3M Innovative Properties Company | Pleated multi-layer filter media and cartridge |
| WO2007024825A2 (en) * | 2005-08-26 | 2007-03-01 | Genway Biotech, Inc. | Immunoaffinity separation and analysis compositions and methods |
| US8834918B2 (en) * | 2007-01-22 | 2014-09-16 | Wisconsin Alumni Research Foundation | Modified multilayered film |
| US8071210B2 (en) * | 2007-10-09 | 2011-12-06 | Wiscousin Alumni Research Foundation | Covalent assembly of ultrathin polymeric films |
| US8710111B2 (en) | 2007-11-09 | 2014-04-29 | 3M Innovative Properties Company | Porous polymeric resins |
| US8592493B2 (en) * | 2008-06-26 | 2013-11-26 | 3M Innovative Properties Company | Solid support with a grafted chain |
| WO2019097446A1 (en) | 2017-11-16 | 2019-05-23 | 3M Innovative Properties Company | Polymer matrix composites comprising functional particles and methods of making the same |
| US10913834B2 (en) | 2017-11-16 | 2021-02-09 | 3M Innovative Properties Company | Polymer matrix composites comprising indicator particles and methods of making the same |
| US10836873B2 (en) | 2017-11-16 | 2020-11-17 | 3M Innovative Properties Company | Polymer matrix composites comprising thermally insulating particles and methods of making the same |
| US11732104B2 (en) | 2017-11-16 | 2023-08-22 | 3M Innovative Properties Company | Polymer matrix composites comprising dielectric particles and methods of making the same |
| US10927228B2 (en) | 2017-11-16 | 2021-02-23 | 3M Innovative Properties Company | Polymer matrix composites comprising intumescent particles and methods of making the same |
| KR102722514B1 (ko) | 2017-11-16 | 2024-10-29 | 쓰리엠 이노베이티브 프로퍼티즈 캄파니 | 중합체 매트릭스 복합재의 제조 방법 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965204A (en) | 1984-02-06 | 1990-10-23 | The Johns Hopkins University | Human stem cells and monoclonal antibodies |
| US5215927A (en) | 1986-01-30 | 1993-06-01 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
| US5336742A (en) | 1987-03-13 | 1994-08-09 | Minnesota Mining And Manufacturing Company | Polymeric supports |
| US5013795A (en) | 1989-04-10 | 1991-05-07 | Minnesota Mining And Manufacturing Company | Azlactone graft copolymers |
| US5262484A (en) | 1989-04-10 | 1993-11-16 | Minnesota Mining And Manufacturing Company | Azlactone graft copolymers |
| US5200471A (en) | 1990-11-05 | 1993-04-06 | Minnesota Mining And Manufacturing Company | Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same |
| NO173481C (no) | 1991-09-11 | 1993-12-22 | Sinvent As | Anlegg for oppdrett og lagring av fisk o.l. i sjoeen |
| US5993935A (en) * | 1991-10-11 | 1999-11-30 | 3M Innovative Properties Company | Covalently reactive particles incorporated in a continous porous matrix |
| US5344701A (en) | 1992-06-09 | 1994-09-06 | Minnesota Mining And Manufacturing Company | Porous supports having azlactone-functional surfaces |
| EP0647232B1 (en) | 1992-06-23 | 1999-04-28 | Minnesota Mining And Manufacturing Company | Deproteinization with azlactone-functional supports |
| US5292514A (en) | 1992-06-24 | 1994-03-08 | Minnesota Mining And Manufacturing Company | Azlactone-functional substrates, corneal prostheses, and manufacture and use thereof |
| EP0693085B1 (en) | 1993-03-29 | 2001-10-31 | Minnesota Mining And Manufacturing Company | Method of coupling ligands within porous supports (p.e. azlactone) and uses thereof |
| US5408002A (en) | 1993-09-09 | 1995-04-18 | Minnesota Mining And Manufacturing Company | Azlactone-functional polymer blends, articles produced therefrom and methods for preparing both |
| US5561097A (en) | 1994-04-28 | 1996-10-01 | Minnesota Mining And Manufacturing Company | Method of controlling density of ligand coupled onto supports and products produced therefrom |
| US5986076A (en) * | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| US5510421A (en) | 1994-05-26 | 1996-04-23 | Minnesota Mining And Manufacturing Company | Azlactone-functional membranes and methods of preparing and using same |
| US5968753A (en) | 1994-06-14 | 1999-10-19 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| US6379952B1 (en) * | 1999-02-01 | 2002-04-30 | 3M Innovative Properties Company | Method for cell selection utilizing azlactone-functional supports |
-
1999
- 1999-02-01 US US09/240,829 patent/US6379952B1/en not_active Expired - Lifetime
- 1999-04-30 DE DE69920972T patent/DE69920972T2/de not_active Expired - Lifetime
- 1999-04-30 JP JP2000596375A patent/JP2002535016A/ja active Pending
- 1999-04-30 CN CNB998159506A patent/CN1141582C/zh not_active Expired - Lifetime
- 1999-04-30 AU AU38752/99A patent/AU3875299A/en not_active Abandoned
- 1999-04-30 EP EP99921573A patent/EP1149289B1/en not_active Expired - Lifetime
- 1999-04-30 WO PCT/US1999/009497 patent/WO2000045174A1/en not_active Ceased
-
2002
- 2002-03-01 US US10/087,555 patent/US20020150951A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1149289A1 (en) | 2001-10-31 |
| WO2000045174A1 (en) | 2000-08-03 |
| CN1141582C (zh) | 2004-03-10 |
| DE69920972D1 (de) | 2004-11-11 |
| AU3875299A (en) | 2000-08-18 |
| US20020150951A1 (en) | 2002-10-17 |
| US6379952B1 (en) | 2002-04-30 |
| CN1334923A (zh) | 2002-02-06 |
| DE69920972T2 (de) | 2005-10-20 |
| EP1149289B1 (en) | 2004-10-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002535016A (ja) | アズラクトン官能性担体を使用したホールセルの選択 | |
| US7727710B2 (en) | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface | |
| US4035316A (en) | Cell specific, variable density, polymer microspheres | |
| Kumar et al. | Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture | |
| Ugelstad et al. | Preparation and biochemical and biomedical applications of new monosized polymer particles | |
| KR19990035924A (ko) | 폴리비닐 알코올을 토대로 한 자성 중합체 입자,그것의 제조방법 및 용도 | |
| JPH0664064B2 (ja) | 免疫精製における細胞に適用しうる分離のための製品 | |
| JP2001512565A (ja) | 疎水性表面への細胞及び生体分子の接着を調節するための組成物及び方法 | |
| JPH0560759A (ja) | スクシンイミド含有ポリマー由来の生物学的活性試薬の製造方法及びそれを含んでなる分析要素並びにその用途 | |
| Ugelstad et al. | Biochemical and biomedical application of monodisperse polymer particles | |
| JP2002098676A (ja) | 分離材及び分離・回収方法 | |
| AU5439794A (en) | Artificial antibodies, method of producing the same and use thereof | |
| Ujam et al. | Isolation of monocytes from human peripheral blood using immuno‐affinity expanded‐bed adsorption | |
| JP3441530B2 (ja) | 温度応答型分離材料及びその製造方法 | |
| JPH059229A (ja) | スクシンイミド含有ポリマー並びにそれらより製造されるラテツクス | |
| JP3441496B2 (ja) | アフィニティー分離材料 | |
| Mandrusov et al. | Membrane-based cell affinity chromatography to retrieve viable cells | |
| JPH08108069A (ja) | 幹細胞分離方法 | |
| JP3311831B2 (ja) | アルデヒド含有ポリマーから製造した生物学的活性試薬、検査キット、分析要素及び使用方法 | |
| JPH07113799A (ja) | 細胞の選択的分離方法 | |
| JP3970339B2 (ja) | 細胞分離用材料 | |
| AU636001B2 (en) | Method of covalently bonding proteins to hydrophilic surfaces | |
| JPH0558707B2 (https=) | ||
| JPH06269499A (ja) | 細胞分離方法及びその装置 | |
| JPH07120452A (ja) | 細胞の選択的分離方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20060428 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060428 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090519 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090819 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20100223 |