DE69920972T2 - Selektion ganzer zellen unter verwendung von azlacton-funktionalisierten trägern - Google Patents
Selektion ganzer zellen unter verwendung von azlacton-funktionalisierten trägern Download PDFInfo
- Publication number
- DE69920972T2 DE69920972T2 DE69920972T DE69920972T DE69920972T2 DE 69920972 T2 DE69920972 T2 DE 69920972T2 DE 69920972 T DE69920972 T DE 69920972T DE 69920972 T DE69920972 T DE 69920972T DE 69920972 T2 DE69920972 T2 DE 69920972T2
- Authority
- DE
- Germany
- Prior art keywords
- whole cells
- carrier
- azlactone
- cells
- biologically active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000969 carrier Substances 0.000 title claims description 40
- IEJPPSMHUUQABK-UHFFFAOYSA-N 2,4-diphenyl-4h-1,3-oxazol-5-one Chemical group O=C1OC(C=2C=CC=CC=2)=NC1C1=CC=CC=C1 IEJPPSMHUUQABK-UHFFFAOYSA-N 0.000 claims description 52
- 238000000034 method Methods 0.000 claims description 36
- 239000000203 mixture Substances 0.000 claims description 19
- 108090001008 Avidin Proteins 0.000 claims description 16
- 239000011324 bead Substances 0.000 claims description 16
- 229940088623 biologically active substance Drugs 0.000 claims description 16
- 239000000427 antigen Substances 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 102000004169 proteins and genes Human genes 0.000 claims description 14
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 13
- 230000003993 interaction Effects 0.000 claims description 11
- 239000013543 active substance Substances 0.000 claims description 10
- 239000000178 monomer Substances 0.000 claims description 8
- 210000005259 peripheral blood Anatomy 0.000 claims description 8
- 239000011886 peripheral blood Substances 0.000 claims description 8
- 229920005601 base polymer Polymers 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 230000009871 nonspecific binding Effects 0.000 claims description 7
- 238000009739 binding Methods 0.000 claims description 6
- 238000001212 derivatisation Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 230000027455 binding Effects 0.000 claims description 5
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- 239000004971 Cross linker Substances 0.000 claims description 4
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- 238000010557 suspension polymerization reaction Methods 0.000 claims description 4
- 230000001588 bifunctional effect Effects 0.000 claims description 3
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- 238000012216 screening Methods 0.000 claims description 2
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- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 7
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 7
- 239000003446 ligand Substances 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 6
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
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- 210000000130 stem cell Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000012050 conventional carrier Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- WHNPOQXWAMXPTA-UHFFFAOYSA-N 3-methylbut-2-enamide Chemical compound CC(C)=CC(N)=O WHNPOQXWAMXPTA-UHFFFAOYSA-N 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 125000004432 carbon atom Chemical group C* 0.000 description 3
- 238000003320 cell separation method Methods 0.000 description 3
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- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229920001917 Ficoll Polymers 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- -1 antibodies Proteins 0.000 description 2
- 210000000612 antigen-presenting cell Anatomy 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000005497 microtitration Methods 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 125000006413 ring segment Chemical group 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000010414 supernatant solution Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- YUDNXDTXQPYKCA-YDHLFZDLSA-N (4-nitrophenyl) 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate Chemical compound C1=CC([N+](=O)[O-])=CC=C1OC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1 YUDNXDTXQPYKCA-YDHLFZDLSA-N 0.000 description 1
- FPBFWDHYZMVTJD-UHFFFAOYSA-N 2-methyl-2-(prop-2-enoylamino)propanoic acid Chemical compound OC(=O)C(C)(C)NC(=O)C=C FPBFWDHYZMVTJD-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
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- 125000000217 alkyl group Chemical group 0.000 description 1
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- 125000002837 carbocyclic group Chemical group 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
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- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000009295 crossflow filtration Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 125000000753 cycloalkyl group Chemical group 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 238000011156 evaluation Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000010559 graft polymerization reaction Methods 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 210000001821 langerhans cell Anatomy 0.000 description 1
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- 210000004185 liver Anatomy 0.000 description 1
- 230000000998 lymphohematopoietic effect Effects 0.000 description 1
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- 239000011159 matrix material Substances 0.000 description 1
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- 102000039446 nucleic acids Human genes 0.000 description 1
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- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
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- 239000002243 precursor Substances 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
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- 229910000077 silane Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
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- 241000894007 species Species 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
- Y10S530/816—Attached to the carrier via a bridging agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T442/00—Fabric [woven, knitted, or nonwoven textile or cloth, etc.]
- Y10T442/20—Coated or impregnated woven, knit, or nonwoven fabric which is not [a] associated with another preformed layer or fiber layer or, [b] with respect to woven and knit, characterized, respectively, by a particular or differential weave or knit, wherein the coating or impregnation is neither a foamed material nor a free metal or alloy layer
- Y10T442/2525—Coating or impregnation functions biologically [e.g., insect repellent, antiseptic, insecticide, bactericide, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US240829 | 1999-02-01 | ||
| US09/240,829 US6379952B1 (en) | 1999-02-01 | 1999-02-01 | Method for cell selection utilizing azlactone-functional supports |
| PCT/US1999/009497 WO2000045174A1 (en) | 1999-02-01 | 1999-04-30 | Whole cell selection utilizing azlactone-functional supports |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69920972D1 DE69920972D1 (de) | 2004-11-11 |
| DE69920972T2 true DE69920972T2 (de) | 2005-10-20 |
Family
ID=22908111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69920972T Expired - Lifetime DE69920972T2 (de) | 1999-02-01 | 1999-04-30 | Selektion ganzer zellen unter verwendung von azlacton-funktionalisierten trägern |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6379952B1 (https=) |
| EP (1) | EP1149289B1 (https=) |
| JP (1) | JP2002535016A (https=) |
| CN (1) | CN1141582C (https=) |
| AU (1) | AU3875299A (https=) |
| DE (1) | DE69920972T2 (https=) |
| WO (1) | WO2000045174A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6379952B1 (en) * | 1999-02-01 | 2002-04-30 | 3M Innovative Properties Company | Method for cell selection utilizing azlactone-functional supports |
| US20030040125A1 (en) * | 2001-08-21 | 2003-02-27 | 3M Innovative Properties Company | Methods for performing immunological assays |
| US7883720B2 (en) | 2003-07-09 | 2011-02-08 | Wisconsin Alumni Research Foundation | Charge-dynamic polymers and delivery of anionic compounds |
| WO2006044532A1 (en) * | 2004-10-15 | 2006-04-27 | 3M Innovative Properties Company | Pleated multi-layer filter media and cartridge |
| WO2007024825A2 (en) * | 2005-08-26 | 2007-03-01 | Genway Biotech, Inc. | Immunoaffinity separation and analysis compositions and methods |
| US8834918B2 (en) * | 2007-01-22 | 2014-09-16 | Wisconsin Alumni Research Foundation | Modified multilayered film |
| US8071210B2 (en) * | 2007-10-09 | 2011-12-06 | Wiscousin Alumni Research Foundation | Covalent assembly of ultrathin polymeric films |
| US8710111B2 (en) | 2007-11-09 | 2014-04-29 | 3M Innovative Properties Company | Porous polymeric resins |
| US8592493B2 (en) * | 2008-06-26 | 2013-11-26 | 3M Innovative Properties Company | Solid support with a grafted chain |
| WO2019097446A1 (en) | 2017-11-16 | 2019-05-23 | 3M Innovative Properties Company | Polymer matrix composites comprising functional particles and methods of making the same |
| US10913834B2 (en) | 2017-11-16 | 2021-02-09 | 3M Innovative Properties Company | Polymer matrix composites comprising indicator particles and methods of making the same |
| US10836873B2 (en) | 2017-11-16 | 2020-11-17 | 3M Innovative Properties Company | Polymer matrix composites comprising thermally insulating particles and methods of making the same |
| US11732104B2 (en) | 2017-11-16 | 2023-08-22 | 3M Innovative Properties Company | Polymer matrix composites comprising dielectric particles and methods of making the same |
| US10927228B2 (en) | 2017-11-16 | 2021-02-23 | 3M Innovative Properties Company | Polymer matrix composites comprising intumescent particles and methods of making the same |
| KR102722514B1 (ko) | 2017-11-16 | 2024-10-29 | 쓰리엠 이노베이티브 프로퍼티즈 캄파니 | 중합체 매트릭스 복합재의 제조 방법 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965204A (en) | 1984-02-06 | 1990-10-23 | The Johns Hopkins University | Human stem cells and monoclonal antibodies |
| US5215927A (en) | 1986-01-30 | 1993-06-01 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
| US5336742A (en) | 1987-03-13 | 1994-08-09 | Minnesota Mining And Manufacturing Company | Polymeric supports |
| US5013795A (en) | 1989-04-10 | 1991-05-07 | Minnesota Mining And Manufacturing Company | Azlactone graft copolymers |
| US5262484A (en) | 1989-04-10 | 1993-11-16 | Minnesota Mining And Manufacturing Company | Azlactone graft copolymers |
| US5200471A (en) | 1990-11-05 | 1993-04-06 | Minnesota Mining And Manufacturing Company | Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same |
| NO173481C (no) | 1991-09-11 | 1993-12-22 | Sinvent As | Anlegg for oppdrett og lagring av fisk o.l. i sjoeen |
| US5993935A (en) * | 1991-10-11 | 1999-11-30 | 3M Innovative Properties Company | Covalently reactive particles incorporated in a continous porous matrix |
| US5344701A (en) | 1992-06-09 | 1994-09-06 | Minnesota Mining And Manufacturing Company | Porous supports having azlactone-functional surfaces |
| EP0647232B1 (en) | 1992-06-23 | 1999-04-28 | Minnesota Mining And Manufacturing Company | Deproteinization with azlactone-functional supports |
| US5292514A (en) | 1992-06-24 | 1994-03-08 | Minnesota Mining And Manufacturing Company | Azlactone-functional substrates, corneal prostheses, and manufacture and use thereof |
| EP0693085B1 (en) | 1993-03-29 | 2001-10-31 | Minnesota Mining And Manufacturing Company | Method of coupling ligands within porous supports (p.e. azlactone) and uses thereof |
| US5408002A (en) | 1993-09-09 | 1995-04-18 | Minnesota Mining And Manufacturing Company | Azlactone-functional polymer blends, articles produced therefrom and methods for preparing both |
| US5561097A (en) | 1994-04-28 | 1996-10-01 | Minnesota Mining And Manufacturing Company | Method of controlling density of ligand coupled onto supports and products produced therefrom |
| US5986076A (en) * | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| US5510421A (en) | 1994-05-26 | 1996-04-23 | Minnesota Mining And Manufacturing Company | Azlactone-functional membranes and methods of preparing and using same |
| US5968753A (en) | 1994-06-14 | 1999-10-19 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| US6379952B1 (en) * | 1999-02-01 | 2002-04-30 | 3M Innovative Properties Company | Method for cell selection utilizing azlactone-functional supports |
-
1999
- 1999-02-01 US US09/240,829 patent/US6379952B1/en not_active Expired - Lifetime
- 1999-04-30 DE DE69920972T patent/DE69920972T2/de not_active Expired - Lifetime
- 1999-04-30 JP JP2000596375A patent/JP2002535016A/ja active Pending
- 1999-04-30 CN CNB998159506A patent/CN1141582C/zh not_active Expired - Lifetime
- 1999-04-30 AU AU38752/99A patent/AU3875299A/en not_active Abandoned
- 1999-04-30 EP EP99921573A patent/EP1149289B1/en not_active Expired - Lifetime
- 1999-04-30 WO PCT/US1999/009497 patent/WO2000045174A1/en not_active Ceased
-
2002
- 2002-03-01 US US10/087,555 patent/US20020150951A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1149289A1 (en) | 2001-10-31 |
| WO2000045174A1 (en) | 2000-08-03 |
| CN1141582C (zh) | 2004-03-10 |
| DE69920972D1 (de) | 2004-11-11 |
| AU3875299A (en) | 2000-08-18 |
| US20020150951A1 (en) | 2002-10-17 |
| US6379952B1 (en) | 2002-04-30 |
| CN1334923A (zh) | 2002-02-06 |
| JP2002535016A (ja) | 2002-10-22 |
| EP1149289B1 (en) | 2004-10-06 |
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