JP2002523020A - 繊維状タンパク質およびその製造 - Google Patents
繊維状タンパク質およびその製造Info
- Publication number
- JP2002523020A JP2002523020A JP2000563769A JP2000563769A JP2002523020A JP 2002523020 A JP2002523020 A JP 2002523020A JP 2000563769 A JP2000563769 A JP 2000563769A JP 2000563769 A JP2000563769 A JP 2000563769A JP 2002523020 A JP2002523020 A JP 2002523020A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- precursor
- plant cell
- fibrous
- fibrous protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000034240 fibrous proteins Human genes 0.000 title claims abstract description 63
- 108091005899 fibrous proteins Proteins 0.000 title claims abstract description 63
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 239000002243 precursor Substances 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 37
- 230000014509 gene expression Effects 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 56
- 102000004169 proteins and genes Human genes 0.000 claims description 53
- 108010014258 Elastin Proteins 0.000 claims description 21
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 14
- 239000004472 Lysine Substances 0.000 claims description 14
- 102000004316 Oxidoreductases Human genes 0.000 claims description 14
- 108090000854 Oxidoreductases Proteins 0.000 claims description 14
- 102000016942 Elastin Human genes 0.000 claims description 12
- 229920002549 elastin Polymers 0.000 claims description 12
- 239000012634 fragment Substances 0.000 claims description 10
- 102100033167 Elastin Human genes 0.000 claims description 9
- 108010035532 Collagen Proteins 0.000 claims description 8
- 102000008186 Collagen Human genes 0.000 claims description 8
- 229920001436 collagen Polymers 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 7
- 230000002062 proliferating effect Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 108010050808 Procollagen Proteins 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 description 44
- 210000004027 cell Anatomy 0.000 description 31
- 244000061456 Solanum tuberosum Species 0.000 description 12
- 239000013604 expression vector Substances 0.000 description 10
- 108010077465 Tropocollagen Proteins 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 5
- 102000035195 Peptidases Human genes 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 230000004807 localization Effects 0.000 description 4
- 235000019833 protease Nutrition 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000004132 cross linking Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 102220201851 rs143406017 Human genes 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 2
- 229960003669 carbenicillin Drugs 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 101100116283 Arabidopsis thaliana DD11 gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 102100033029 Carbonic anhydrase-related protein 11 Human genes 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 102100036738 Guanine nucleotide-binding protein subunit alpha-11 Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000695352 Homo sapiens Bone morphogenetic protein 1 Proteins 0.000 description 1
- 101000867841 Homo sapiens Carbonic anhydrase-related protein 11 Proteins 0.000 description 1
- 101000851054 Homo sapiens Elastin Proteins 0.000 description 1
- 101100283445 Homo sapiens GNA11 gene Proteins 0.000 description 1
- 101001075218 Homo sapiens Gastrokine-1 Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 101710089395 Oleosin Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010064622 Procollagen N-Endopeptidase Proteins 0.000 description 1
- 102000015339 Procollagen N-endopeptidase Human genes 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 101150049515 bla gene Proteins 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 235000000141 cabbage gum Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 235000016213 coffee Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 102000045875 human BMP1 Human genes 0.000 description 1
- 102000054289 human ELN Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000020978 protein processing Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19834909A DE19834909A1 (de) | 1998-08-03 | 1998-08-03 | Faserproteine und ihre Herstellung |
| DE19834909.2 | 1998-08-03 | ||
| PCT/DE1999/002359 WO2000008142A2 (fr) | 1998-08-03 | 1999-08-03 | Proteine de fibre et sa production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2002523020A true JP2002523020A (ja) | 2002-07-30 |
Family
ID=7876235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000563769A Pending JP2002523020A (ja) | 1998-08-03 | 1999-08-03 | 繊維状タンパク質およびその製造 |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1100915A2 (fr) |
| JP (1) | JP2002523020A (fr) |
| AU (1) | AU758727B2 (fr) |
| CA (1) | CA2336064A1 (fr) |
| DE (1) | DE19834909A1 (fr) |
| IL (1) | IL140549A0 (fr) |
| WO (1) | WO2000008142A2 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023194333A1 (fr) | 2022-04-04 | 2023-10-12 | Swiftpharma Bv | Protéines de collagène recombinantes renforcées par de la soie d'araignée produites dans des plantes et leur utilisation |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997004123A1 (fr) * | 1995-07-19 | 1997-02-06 | Gel Tech Group Inc. | Production de collagene par des plantes |
| US5928922A (en) * | 1996-04-10 | 1999-07-27 | Academy Of Finland | α2 subunit of prolyl-4-hydroxylase, nucleic acid sequences encoding such subunit and methods for producing the same |
| AU3056697A (en) * | 1996-04-12 | 1997-11-07 | Academy Of Finland | Synthesis of human procollagens and collagens in recombinant dna systems |
| AUPO156596A0 (en) * | 1996-08-09 | 1996-09-05 | University Of Sydney, The | Synthetic polynucleotides |
| EP0939826A2 (fr) * | 1996-08-15 | 1999-09-08 | Agrivax Incorporated | Administration d'antigenes tolerogeniques via des plantes comestibles ou des produits derives de plantes |
| FR2757874B1 (fr) * | 1996-12-17 | 2003-04-25 | Biocem | Collagenes recombinants et proteines derivees produits par les plantes, leurs procedes d'obtention et leurs utilisations |
-
1998
- 1998-08-03 DE DE19834909A patent/DE19834909A1/de not_active Withdrawn
-
1999
- 1999-08-03 WO PCT/DE1999/002359 patent/WO2000008142A2/fr not_active Application Discontinuation
- 1999-08-03 JP JP2000563769A patent/JP2002523020A/ja active Pending
- 1999-08-03 CA CA002336064A patent/CA2336064A1/fr not_active Abandoned
- 1999-08-03 IL IL14054999A patent/IL140549A0/xx unknown
- 1999-08-03 AU AU63250/99A patent/AU758727B2/en not_active Ceased
- 1999-08-03 EP EP99950481A patent/EP1100915A2/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000008142A2 (fr) | 2000-02-17 |
| AU6325099A (en) | 2000-02-28 |
| WO2000008142A3 (fr) | 2000-06-08 |
| DE19834909A1 (de) | 2000-02-17 |
| CA2336064A1 (fr) | 2000-02-17 |
| EP1100915A2 (fr) | 2001-05-23 |
| IL140549A0 (en) | 2002-02-10 |
| AU758727B2 (en) | 2003-03-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20040518 |