JP2002506419A - A型肝炎ウイルスポリタンパク質の抗原反応性領域 - Google Patents
A型肝炎ウイルスポリタンパク質の抗原反応性領域Info
- Publication number
- JP2002506419A JP2002506419A JP53834397A JP53834397A JP2002506419A JP 2002506419 A JP2002506419 A JP 2002506419A JP 53834397 A JP53834397 A JP 53834397A JP 53834397 A JP53834397 A JP 53834397A JP 2002506419 A JP2002506419 A JP 2002506419A
- Authority
- JP
- Japan
- Prior art keywords
- peptide
- immunogenic
- hav
- immunogenic peptide
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
- C12N2770/32422—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチドが アミノ酸1〜約23に相当するHAVのVP4タンパク質のアミノ酸配列の一部 分とアミノ酸24〜約245に相当するHAVのVP2タンパク質のアミノ酸配 列の一部分とに実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 2. 前記免疫原性ペプチドが下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項1に記載の免疫原性ペプチド。 3. 前記免疫原性ペプチドが下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項1に記載の免疫原性ペプチド。 4. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項1に 記載の免疫原性ペプチド。 5. 前記担体タンパク質が血清アルブミン、アオガイヘモシアニン、ジフテ リア毒素、破傷風毒素及び合成高分子から成るグループから選択された部材であ る、請求項4に記載の免疫原性ペプチド。 6. さらに製薬学的に容認できる担体を含んでいる、請求項1に記載の免疫 原性ペプチド。 7. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチドが アミノ酸246〜約491に相当するHAVのVP3タンパク質の一部分に実質 的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 8. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項7に記載の免疫原性ペプチド。 9. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項7に記載の免疫原性ペプチド。 10. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項7 に記載の免疫原性ペプチド。 11. さらに製薬学的に容認できる担体を含んでいる、請求項7に記載の免 疫原性ペプチド。 12. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸492〜約791に相当するHAVのVP1タンパク質の一部分に実 質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 13. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項12に記載の免疫原性ペプチド。 14. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる免疫原性ペプチド。 15. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項1 2に記載の免疫原性ペプチド。 16. さらに製薬学的に容認できる担体を含んでいる、請求項12に記載の 免疫原性ペプチド。 17. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸792〜約980に相当するHAVのP2Aタンパク質の一部分に実 質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 18. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項17に記載の免疫原性ペプチド。 19. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項17に記載の免疫原性ペプチド。 20. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項1 7に記載の免疫原性ペプチド。 21. さらに製薬学的に容認できる担体を含んでいる、請求項17に記載の 免疫原性ペプチド。 22. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸981〜約1087に相当するHAVのP2Bタンパク質の一部分に 実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 23. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項22に記載の免疫原性HAVペプチド。 24. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド が、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項22に記載の免疫原性ペプチド。 25. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項2 2に記載の免疫原性ペプチド。 26. さらに製薬学的に容認できる担体を含んでいる、請求項22に記載の 免疫原性ペプチド。 27. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸1088〜約1422に相当するHAVのP2Cタンパク質の一部分 に実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 28. 前記免疫原性ペプチドが、下記の及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項27に記載の免疫原性ペプチド。 29. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項27に記載の免疫原性ペプチド。 30. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項2 7に記載の免疫原性ペプチド。 31. さらに製薬学的に容認できる担体を含んでいる、請求項27に記載の 免疫原性ペプチド。 32. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸1423〜約1496に相当するHAVのP3Aタンパク質の一部分 に実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 33. 前記免疫原性ペプチドが、下記の及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項27に記載の免疫原性ペプチド。 34. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項32に記載の免疫原性ペプチド。 35. 前記免疫原性ペプチドが担体タンパク質に抱 合されている、請求項32に記載の免疫原性ペプチド。 36. さらに製薬学的に容認できる担体を含んでいる、請求項32に記載の 免疫原性ペプチド。 37. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸1497〜約1519に相当するHAVのP3Bタンパク質の一部分 に実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 38. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項27に記載の免疫原性ペプチド。 39. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項27に記載の免疫原性ペプチド。 40. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項3 7に記載の免疫原性ペプチド。 41. さらに製薬学的に容認できる担体を含んでいる、請求項37に記載の 免疫原性ペプチド。 42. 単離された免疫原性HAVペプチドであって、前記免疫原性ペプチド がアミノ酸1520〜約1738に相当するHAVのP3Cタンパク質の一部分 に実質的に類似するアミノ酸配列を含んでいる免疫原性ペプチド。 43. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたペプチドと特異的に免 疫反応性の抗体に結合する、請求項27に記載の免疫原性ペプチド。 44. 前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる、請求項42に記載の免疫原性ペプチド。 45. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項4 2に記載の免疫原性ペプチド。 46. さらに製薬学的に容認できる担体を含んでい る、請求項42に記載の免疫原性ペプチド。 47. 免疫学的組成物であって、前記免疫学的組成物が製薬学的に容認でき る担体と本発明の単離された免疫原性HAVペプチドとを哺乳類においてHAV への保護免疫反応を誘発するために十分な量で含有しており、前記免疫原性ペプ チドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる免疫学的組成物。 48. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項4 7に記載の組成物。 49. 前記担体タンパク質が血清アルブミン、アオガイヘモシアニン、ジフ テリア毒素、破傷風毒素及び合成高分子から成るグループから選択された部材で ある、請求項48に記載の組成物。 50. 哺乳類においてHAVへの免疫反応を誘発する方法であって、前記方 法が製薬学的に容認できる担体と本発明の単離された免疫原性HAVペプチドと を含んでいる免疫学的に有効な量の製薬学的組成物を哺乳類に投与することを含 んでおり、前記免疫原性ペプチドが、 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる方法。 51. 前記免疫原性ペプチドが担体タンパク質に抱合されている、請求項5 0に記載の方法。 52. 哺乳類血清中のHAVに対する抗体の存在を検出する方法であって 、前記方法が、 (a)本発明の単離された免疫原性HAVペプチドを哺乳類血清からの抗体と接 触させること;このとき前記免疫原性ペプチドは、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる;及び (b)免疫原性ペプチドと抗体との複合体の形成を検出すること、 を含んでいる方法。 53. さらに第2免疫原性HAVペプチドを哺乳類血清からの抗体と接触さ せることを含んでおり、このとき前記第2免疫原性ペプチドが独立して下記の 及びそれらの保存的変種を含むグループから選択されている、請求項52に記載 の方法。 54. 前記免疫原性ペプチドが下記のアミノ酸配列 及びその保存的変種を含んでいる、請求項52に記載の方法。 55. さらに下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる免疫原性ペプチドを含んでいる、請求項54に記載の方法。 56. ワクチン誘発性免疫と自然HAV免疫とを識別する方法であって、前 記方法が、 (a)本発明の単離された非構造的免疫原性HAVペプチドを哺乳類血清からの 抗体と接触させること;このとき前記免疫原性ペプチドは、下記の及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる;及び (b)免疫原性ペプチドと抗体との複合体の形成を検出すること、 を含んでおり、このときペプチド抗体複合体の存在が自然HAV免疫を指示する 方法。 57. さらに第2免疫原性HAVペプチドを哺乳類血清からの抗体と接触さ せることを含んでおり、このとき前記第2免疫原性ペプチドが独立して下記の 及びそれらの保存的変種を含むグループから選択されている、請求項56に記載 の方法。 58. 前記免疫原性HAVペプチドが下記のアミノ酸配列 及びその保存的変種を含んでいる、請求項56に記載の方法。 59. さらに下記の及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる免疫原性ペプチドを含んでいる、請求項58に記載の方法。 60. HAVの診断のためのキットであって、前記キットは容器と本発明の 単離された免疫原性HAVペプチドとを含んでおり、前記免疫原性ペプチドが、 下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いるキット。 61. さらに診断テストを実施するための説明資料を含んでいる、請求項6 0に記載のキット。 62. ワクチン誘発性免疫と自然HAV免疫とを識別するためのキットであ って、前記キットが容器と本発明の単離された免疫原性HAVペプチドとを含ん でおり、前記免疫原性ペプチドが、下記の及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いるキット。 63. さらに、ワクチン誘発性免疫と自然HAV免疫とを識別するためのテ ストを実施するための説明資料を含んでいる、請求項62に記載のキット。 64. 単離されたDNA配列であって、前記DNA配列が下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を有して いるHAV免疫原性ペプチドをコードするDNA配列。 65. 免疫原性抱合体であって、前記抱合体が(b)単離された免疫原性H AVペプチドに(a)共有的に付加されている担体タンパク質を含んでおり、前 記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる抱合体。 66. 前記担体タンパク質が血清アルブミン、アオガイヘモシアニン、ジフ テリア毒素、破傷風毒素及び合成高分子から成るグループから選択された部材で ある、請求項65に記載の組成物。 67. 前記担体タンパク質が少なくとも1個のスペーサー分子を通して前記 免疫原性ペプチドに共有的に付加されている、請求項65に記載の抱合体。 68. 前記方法が哺乳類に免疫原性HAVペプチドを投与することを含んで おり、前記免疫原性ペプチドが、下記の 及びそれらの保存的変種から成るグループから選択されたアミノ酸配列を含んで いる方法。
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US7223535B2 (en) | 1996-04-19 | 2007-05-29 | Centers For Disease Control | Synthetic peptides immunoreactive with hepatitis A virus antibodies |
US20080050367A1 (en) | 1998-04-07 | 2008-02-28 | Guriq Basi | Humanized antibodies that recognize beta amyloid peptide |
US7964192B1 (en) | 1997-12-02 | 2011-06-21 | Janssen Alzheimer Immunotherapy | Prevention and treatment of amyloidgenic disease |
US7790856B2 (en) | 1998-04-07 | 2010-09-07 | Janssen Alzheimer Immunotherapy | Humanized antibodies that recognize beta amyloid peptide |
TWI239847B (en) | 1997-12-02 | 2005-09-21 | Elan Pharm Inc | N-terminal fragment of Abeta peptide and an adjuvant for preventing and treating amyloidogenic disease |
AU775557B2 (en) * | 1999-07-15 | 2004-08-05 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Synthetic peptides immunoreactive with hepatitis A virus antibodies |
US6884877B2 (en) * | 2001-10-03 | 2005-04-26 | Florida State University Research Foundation, Inc. | Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods |
MY139983A (en) | 2002-03-12 | 2009-11-30 | Janssen Alzheimer Immunotherap | Humanized antibodies that recognize beta amyloid peptide |
CA2590337C (en) | 2004-12-15 | 2017-07-11 | Neuralab Limited | Humanized amyloid beta antibodies for use in improving cognition |
US8784810B2 (en) | 2006-04-18 | 2014-07-22 | Janssen Alzheimer Immunotherapy | Treatment of amyloidogenic diseases |
US8003097B2 (en) | 2007-04-18 | 2011-08-23 | Janssen Alzheimer Immunotherapy | Treatment of cerebral amyloid angiopathy |
ES2498040T3 (es) | 2007-07-27 | 2014-09-24 | Janssen Alzheimer Immunotherapy | Tratamiento de enfermedades amiloidogénicas con anticuerpos anti-beta humanizados |
JO3076B1 (ar) | 2007-10-17 | 2017-03-15 | Janssen Alzheimer Immunotherap | نظم العلاج المناعي المعتمد على حالة apoe |
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CA1324094C (en) * | 1985-04-03 | 1993-11-09 | Dino Dina | Hepatitis a virus vaccines |
NZ235315A (en) * | 1989-09-19 | 1991-09-25 | Wellcome Found | Chimaeric hepadnavirus core antigen proteins and their construction |
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