JP2002255711A - Antibacterial composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antibacterial composition having excellent antibacterial and bacteriostatic actions on Corynebacterium sp. or Staphylococcus aureus, and to obtain a skin care agent and an anti-hircismus agent each containing this antibacterial composition. SOLUTION: This antibacterial composition contains a sugar fatty acid ester having a 8 to 22C and a para-hydroxybenzoate as active ingredients.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an antibacterial composition, particularly an antibacterial agent exhibiting an excellent bactericidal or bacteriostatic effect against Corynebacterium sp. And Staphylococcus aureus . Composition. The present invention also relates to a skin external preparation containing the above antibacterial composition. Further, the present invention relates to an anti-axillary deodorant containing the antibacterial composition, based on the antibacterial activity of the antibacterial composition against bacteria causing axillary odor.

[0002]

2. Description of the Related Art It is known that unpleasant odor due to sweating is caused by decomposition of naturally odorless and aseptic sweat by microorganisms on the skin surface, to which ammonia in the skin surface and sweat is added. And a more characteristic odor. Especially the axillary odor, which is a unique odor that sticks out of the body odor,
Apocrine sweat, which is secreted from the apocrine sweat glands in the axilla, is caused by decomposition by corynebacterium and staphylococci present on the skin surface. Therefore, in order to suppress the generation of body odor, antiperspirants, antiperspirants, antibacterial agents, antibacterial agents and the like have been conventionally used. However, antiperspirants and antiperspirants are not very effective in people who secrete a large amount of sweat. In particular, antiperspirants consisting of aluminum salts such as aluminum hydroxychloride (aluminum chloride hydrate) are not suitable for their protein. There is a problem that the skin is highly irritating due to the denaturing effect. In addition, although antibacterial agents and antibiotics such as benzethonium chloride and benzalkonium chloride are highly effective, not only microorganisms that cause body odor but also microorganisms that form the skin microbiota are sterilized or bacteriostatic. There is a danger that the skin's natural self-cleansing action may be impaired due to the formation of a natural skin flora.

Therefore, in order to develop a method for preventing an unpleasant body odor, even if it is used for a long period of time, it does not adversely affect the healthy skin microflora as much as possible, and causes bacteria that cause unpleasant odor. It is necessary to find an antibacterial agent having an action of selectively disinfecting or bacteriostatic.

[0004] Antibiotics and antibacterial agents have conventionally been used as a method for sterilizing and removing S. aeruginosa such as Staphylococcus aureus, and have the same problems as described above.

[0005] On the other hand, sucrose fatty acid esters are known to have a bacteriostatic action against heat-resistant spore-forming spores. Therefore, sucrose fatty acid esters have been used as preservatives and preservatives in the field of foods and cosmetics. It is an ionic surfactant. Specifically, according to JP-A-10-70971, sucrose fatty acid esters are Bacillus coagulans , B. circulans , B. ce .
reus , B. licheniformis , B. stearothermophilus , B. sub
tilis , B. polymyxa , Clostridium sporogenes , C. perfr
ingens , C. bifementans , C. butyricum , C. boturinum ,
C. pasteurianum , C. thermaceticum , C. thermosacharoly
ticum , C. thermohydrosulfricum , and Desulfotomaculu
m has antibacterial action against thermostable spore-forming bacteria such as Nigrificans, the spoilage of food due to these microorganisms have been described that can significantly prevent and Hei 1-311
According to Japanese Patent No. 014, sucrose fatty acid ester is Propioni
It is described that it has an antibacterial activity against resident bacteria belonging to the genus Propionibacterium such as bacterium acnes and Propionibacterium avidam , and is useful for preventing acne deterioration and skin inflammation.

[0006] Further, paraoxybenzoic acid esters have an action of destroying cell membranes and denaturing proteins, so that they can be used as preservatives, preservatives, stabilizers, bactericides, etc. as pharmaceuticals (eg, eye drops and nasal drops), It is a compound widely used in quasi-drugs, cosmetics (cosmetics for hair, basic cosmetics, makeup cosmetics, bath additives) and foods. Paraoxybenzoate is a compound that has little irritation to the skin and is relatively skin-friendly, but some people show hypersensitivity to the compound.

[0007] As described above, the antibacterial properties of sucrose fatty acid esters and paraoxybenzoic acid esters are conventionally known, but by using both of them, Corynebacterium sp. And Staphylococcus aureus can be used. ) Are not known to exhibit an excellent bactericidal action against certain microorganisms.

[0008]

SUMMARY OF THE INVENTION An object of the present invention is to provide an antibacterial composition. More specifically, the present invention provides an antibacterial property that exhibits an excellent bactericidal effect against Corynebacterium sp., Particularly an axillary odor bacterium, which is a germ causing axillary odor, which is an unpleasant odor among body odors. It is an object of the present invention to provide a composition and the present invention relates to Staphylococcus ( Staphyloco
The purpose of the present invention is to provide an antibacterial composition exhibiting an excellent bactericidal effect against Ccus aureus . Further, the present invention provides an external preparation for skin used for the purpose of suppressing the occurrence of axillary odor by preventing the growth and proliferation of Corynebacterium spp., And for the purpose of preventing suppuration of damaged parts such as wounds. The purpose is to: Still another object of the present invention is to provide an anti-axillary odor agent that prevents an axillary odor based on the above action.

[0009]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to achieve the above object, and have found that paraoxybenzoic acid esters and sucrose esters of fatty acids having 8 to 22 carbon atoms are used in combination. By doing so, it has been found that a small amount exhibits excellent bacteriostatic action against Corynebacterium sp., Especially bacteria causing axillary odor, and furthermore, paraoxybenzoic acid ester is shown against Corynebacterium sp. It has been found that the amount of paraoxybenzoate used can be remarkably reduced by enhancing the bactericidal action possessed by sucrose fatty acid ester. In addition, the present inventors have found that Staphylococc ( Staphylococc) can be obtained by using a combination of a paraoxybenzoate and a sucrose ester of a fatty acid having 8 to 22 carbon atoms.
us aureus ) was found to synergistically enhance the bacteriostatic and bactericidal action. Such a synergistic enhancement of bacteriostatic and bactericidal action by the combined use of paraoxybenzoate and sucrose fatty acid ester is due to Pseudomonas aerugi
nosa ), Bacillus subtilis , E. coli ( E
scherichea coli ), Aspergillus (filamentous fungi) ( Asp
ergillus nigar ), black fungus ( Cladosporium cladospor)
iodides ), Penicillium citrinu
m ), Candida albicans and other microorganisms, and confirmed to be specific to Corynebacterium and Staphylococcus aureus. Through experiments, it has been found that the combination use of the above-mentioned paraoxybenzoic acid ester and sucrose fatty acid ester only acts bacteriostatic on Staphylococcus epidermidis and does not show a bactericidal action. By the way, such Staphylococcus epidermidis ( Staphyloco
ccus epidermidis ) is a resident bacterium of the skin, and it is known that the skin maintains its self-cleansing and protective effects by the skin microflora formed by such resident bacterium. Therefore, the antibacterial composition containing paraoxybenzoic acid ester and sucrose fatty acid ester protects healthy skin microflora, but also causes corynebacterium and aureus that cause axillary odor. It was confirmed that it was effective for selective eradication of E. coli and that it could be applied as a skin-friendly external preparation for skin. The present invention has been developed based on such knowledge.

That is, the present invention is an antibacterial composition described in the following (1) to (5): (1) It contains a sucrose ester of a fatty acid having 8 to 22 carbon atoms and a paraoxybenzoic acid ester or a salt thereof. Antibacterial composition. (2) Corynebacterium spp. (Corynebacterium s
p.), which has an antibacterial action against (p.). (3) Corynebacterium spp. (Corynebacterium s
(p.) is the fungus causing axillary odor. (4) The antibacterial composition according to (1), which has an antibacterial activity against Staphylococcus aureus . (5) The antibacterial composition according to any one of (1) to (4), which is used for skin.

Further, the present invention is a preparation according to the following (6) or (7) containing the above antibacterial composition as an active ingredient: (6) An external preparation for skin, particularly the above antibacterial composition (3) The external preparation for skin contained therein is useful as an external preparation for preventing axillary odor (for axillary odor), and the external preparation for skin containing the antibacterial composition of the above (4) is useful as an external preparation for preventing suppuration. (7) Anti-armpit deodorant.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The antibacterial composition of the present invention is characterized by comprising a sucrose fatty acid ester and a paraoxybenzoic acid ester as active ingredients.

The sucrose fatty acid ester used in the present invention is a sucrose ester of a fatty acid having 8 to 22 carbon atoms. Here, the fatty acid has 8 to 22, preferably 1 carbon atoms.
Mention may be made of from 2 to 18 saturated or unsaturated fatty acids. Specific examples include caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, arachiic acid, behenic acid, oleic acid, and erucic acid. Preferred are lauric acid, myristic acid, palmitic acid and stearic acid. The sucrose fatty acid ester used in the present invention may be composed of one of the above fatty acids, or may be a mixture of two or more of the above fatty acids.

The sucrose fatty acid ester used in the present invention may be a mono-, di-, tri-, tetra-, penta-, hexa-, hepta- or octa-fatty acid ester of sucrose, and may be a mixture of two or more of these. There may be. Preferably 50% monoester per 100% ester composition
% Is desirable, and the proportion is more preferably 70% or more, particularly preferably 75% or more. The HLB is usually in the range of 9 to 20. Preferably it is 11-20, More preferably, it is 15-20.

In the present invention, as the paraoxybenzoic acid ester, those used in the fields of pharmaceuticals, quasi-drugs, cosmetics and foods can be widely used. Specific examples include methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, isopropyl paraoxybenzoate, butyl paraoxybenzoate, isobutyl paraoxybenzoate, and the like. Among them, propyl paraoxybenzoate and butyl paraoxybenzoate are preferable, and propyl paraoxybenzoate is more preferable. In addition, these paraoxybenzoic acid esters may be in the form of a salt, and examples of such salts include alkali metal salts of paraoxybenzoic acid esters such as methyl sodium paraoxybenzoate. The paraoxybenzoic acid esters or salts thereof can be used alone or in any combination of two or more.

The antibacterial composition of the present invention has a bactericidal and bacteriostatic action against Corynebacterium sp. Here, Corynebacterium sp.
There is no particular limitation as long as it is a gram-positive bacillus in the category of the genus, but a bacterium that causes the generation of axillary odor (hereinafter, referred to as
In the present invention, it is also called axillary bacterium. ), Such as axillary odor bacteria Corynebacterium minutissimum ,
Corynebacterium xerosis or Corynebacterium te
Nuis and the like are known. Of these, Corynebact is preferred.
erium minutissimum .

The bactericidal action against Corynebacterium spp. Is as follows. When an antibacterial composition is prepared from sucrose fatty acid ester and paraoxybenzoate, the minimum bactericidal concentration (MBC) is 100 to 1000 ppm, preferably 150 to 500 ppm. Can be shown as

The bacteriostatic effect is as follows: when an antibacterial composition is prepared from a sucrose fatty acid ester and a paraoxybenzoate, the minimum inhibitory concentration (MIC) is 4 to 1%.
It can be given as 00 ppm, preferably 5-40 ppm. The ratio between the sucrose fatty acid ester and the paraoxybenzoic acid ester contained in the antibacterial composition of the present invention can be appropriately adjusted and set so as to retain the bactericidal action and the bacteriostatic action, and is particularly limited. Without
Usually, it can be selected from the range of sucrose fatty acid ester: paraoxybenzoic acid ester = 1: 99 to 99: 1.

Further, the antibacterial composition of the present invention is characterized by exhibiting excellent bactericidal and bacteriostatic activities against Staphylococcus aureus . Its bactericidal action can be shown as a minimum bactericidal concentration (MBC) of 150 to 10000 ppm, preferably 200 to 5000 ppm, when an antibacterial composition is prepared from sucrose fatty acid ester and paraoxybenzoate. Moreover, its bacteriostatic action is as follows: when an antibacterial composition is prepared from sucrose fatty acid ester and paraoxybenzoate, the minimum inhibitory concentration (MIC) is 40 to 1000 pp.
m, preferably 60-500 ppm. The ratio between the sucrose fatty acid ester and the paraoxybenzoic acid ester contained in the antibacterial composition of the present invention can be appropriately adjusted and set so as to retain the bactericidal action and the bacteriostatic action, and is particularly limited. Without the usual sucrose fatty acid ester: p-hydroxybenzoic acid ester =
The ratio can be selected from the range of 1:99 to 99: 1.

The antimicrobial composition of the present invention may contain other antimicrobial components as components other than the above-mentioned sucrose fatty acid ester and paraoxybenzoic acid ester as long as the effects of the present invention are not impaired. Specific examples of such antibacterial components include triclosan, trichlorocarbanilide, benzalkonium chloride, benzethonium chloride, halocarban, chlorhexidine hydrochloride, dihydrofarnesol, isopropylmethylphenol, thymol, and the like.

Since the antibacterial composition of the present invention has an antibacterial action (bactericidal and bacteriostatic action) against Corynebacterium sp. Present on the skin, it can be used as an antibacterial component in skin external preparations. It can be used in combination. In particular, since the antibacterial composition of the present invention exhibits an excellent bactericidal action against axillary odor bacteria, it can be used as an antibacterial component of a skin external preparation for axillary odor. In this case, the mixing ratio of the antibacterial composition of the present invention to the external preparation for skin is such that the sucrose fatty acid ester and the paraoxybenzoate which are the active ingredients exert the antibacterial effect against Corynebacterium spp. The amount of sucrose fatty acid ester in the external preparation for skin may be 0.0004 to 20% by weight, more preferably 0.015 to 5% by weight. It is desirable that the benzoic acid ester is contained in the range of 0.0001 to 1.5% by weight, more preferably 0.015 to 1% by weight.

The antibacterial composition of the present invention can be used by blending it with an external preparation for skin as an antibacterial component because of its antibacterial activity (bactericidal and bacteriostatic activities) against Staphylococcus aureus.
An external preparation for preventing suppuration can be prepared. In this case, the compounding ratio of the antibacterial composition of the present invention to the external preparation for skin is such that the sucrose fatty acid ester and paraoxybenzoate which are the active ingredients exert an antibacterial effect on Staphylococcus aureus. The sucrose fatty acid ester is preferably 0.0004 to 20% by weight, more preferably 0.1 to 5% by weight in the external preparation for skin, but is not particularly limited in this range. 0.004 to 1.5% by weight of ester, more preferably 0.004 to 1.5% by weight.
It is desirable to be contained in the range of 0.5 to 1% by weight.

The present invention also provides a skin external preparation prepared as described above. The external preparation for skin can be widely used in the fields of pharmaceuticals, quasi-drugs, and cosmetics (cosmetics) for the purpose of preventing axillary odor or suppuration. The form is not particularly limited, and creams, ointments, lotions, sprays, aerosols, emulsions, foams, detergents, and sheet preparations in which the above-mentioned antibacterial agent is impregnated in a sheet-like substrate, a patch or a cataplasm, a roll-on It is appropriately prepared in a liquid, semi-solid, cellular, or solid state according to the use form of the agent or the like.

The external preparation for skin includes, in addition to the above-mentioned antibacterial composition containing sucrose fatty acid ester and paraoxybenzoate as components, carriers and preservatives which are usually used in external preparations according to various forms. Agents, stabilizers, suspending agents, pH adjusters, fragrances, surfactants (anionic, cationic, nonionic, amphoteric surfactants), coloring agents, fining agents, viscosity adjusters, bactericidal antibacterial agents, essential oils, etc. Can be blended. As such carriers and additives, those which are safe for the human body are preferred, and those which are not irritating to the skin and the like are more preferred.

As a carrier, for example, in the case of a liquid form,
Water (for example, ion-exchanged water), alcohol (for example, ethanol), and water-soluble solvents (for example, propylene glycol and glycerin). In the case of solid form, various gelling agents (for example, seaweed such as carrageenan and sodium alginate). Polysaccharides; seed polysaccharides such as guar gum and tamarind seed gum; plant sap polysaccharides such as arabic gum and tragacanth gum; fermented polysaccharides such as xanthan gum and gellan gum; proteins such as gelatin; cellulose derivatives such as carboxymethyl cellulose); Examples thereof include inorganic materials such as silica.

As preservatives, benzoic acid, sorbic acid,
Propionic acid, dehydroacetic acid, salicylic acid, edetic acid and salts thereof; sodium sulfite, sodium hyposulfite,
Inorganic salts such as sodium pyrosulfite; plant-derived components such as pectin extract;

Examples of the precipitation inhibitor include fine particles such as silicon dioxide and magnesium stearate, surfactants such as polyoxyethylene hydrogenated castor oil and polyoxyethylene sorbitan monostearate, and gelling agents such as xanthan gum and polyethylene glycol. And the like.

Examples of the pH adjuster include citric acid, fumaric acid, DL-malic acid and salts thereof; carbonates such as sodium hydrogen carbonate and potassium carbonate; phosphates such as phosphoric acid and sodium dihydrogen phosphate. can do.

The above-described various components are merely examples, and the present invention is not limited to these components. That is, in the skin external preparation of the present invention, various components usually used as components of the external preparation can be used, including other additional components.

The antimicrobial composition of the present invention is to sterilize the Corynebacterium genus causing unpleasant odor sources by decomposing sweat and sebaceous secretions, and Staphylococcus epidermidis (Staphyloco
ccusepidermidis ) growth inhibitory (bacteriostatic)
It is useful for preventing the generation of body odor such as axillary odor and sweat odor. Further, the antibacterial composition of the present invention has an excellent bactericidal action against Staphylococcus aureus, which is a pyogenic bacterium, and is therefore useful for preventing suppuration of wounds and the like. Furthermore, the antibacterial composition of the present invention acts only bacteriostatic on Staphylococcus epidermidis, which is a resident bacterium, and has no bactericidal action. Without any adverse effects,
It is useful as a skin-friendly antibacterial component for skin that does not impair the skin's natural self-cleansing function and barrier function.

The external preparation for skin of the present invention, especially the external preparation for axillary odor, has a bactericidal action against Corynebacterium spp., Especially an axillary odor bacterium causing axillary odor, and a bacteriostatic action against Staphylococcus epidermidis. It is excellent in preventing the occurrence of axillary odor and sweat odor. Therefore, the external preparation for skin can also be positioned as a deodorant (body deodorant) used to suppress the generation of axillary odor and sweat odor from another viewpoint, in particular, as an anti-armpit odor.

Furthermore, the antibacterial composition of the present invention is prepared in the form of the above-mentioned external preparation for skin, and is used in the form of directly applied to the skin (skin surface) on which Corynebacterium or Staphylococcus epidermidis is present. Sprays on clothing such as underwear and shirts to which Corynebacterium or Staphylococcus epidermidis adheres (or may adhere) by being prepared in the form of a spray or aerosol, or in the form of a laundry finish or coating agent , Dipping or coating.

When the antibacterial composition of the present invention is used as the latter so-called deodorant for clothing (body deodorant, axillary deodorant), the ratio of the antibacterial composition to be added to the deodorant is as follows: 0.0004 to 2 as the amount of sugar fatty acid ester
0% by weight, preferably 0.1 to 5% by weight, and the amount of paraoxybenzoic acid ester in the range of 0.001 to 3% by weight, more preferably 0.015 to 1% by weight.

The deodorant of the present invention (body deodorant, axillary deodorant) includes, as long as the effects of the present invention are not impaired,
In addition to the above other antibacterial components, known preservatives, preservatives, stabilizers, sedimentation inhibitors, pH adjusters, fragrances, surfactants (anionic, Various additives such as a cationic, nonionic, amphoteric surfactant), a fining agent, a viscosity modifier, and a bactericidal antibacterial agent can be blended. As such carriers and additives, those which are safe for the human body are preferred, and those which are not irritating to the skin and the like are more preferred.

[0035]

The present invention will be described below in more detail with reference to experimental examples and examples. However, the present invention is not limited by the embodiments and the like. Unless otherwise specified, the percentages shown below mean% by weight.

Experimental Example 1 Bactericidal action against various bacteria (MB
C) Sucrose fatty acid ester (fatty acid having 12 to 18 carbon atoms) and p-hydroxybenzoic acid ester (propyl) were mixed at the ratio (weight ratio) shown in Table 1, and this was mixed with the antibacterial composition for testing (No. No. 1 to No. 12) and various bacteria (armpit odor bacteria)
[ Corynebacterium minutissimum ] (IFO15361), Staphylococcus aureus [IFO13276], Staphylococcus epidermidis [ATCC1222]
The minimum bactericidal concentration (MBC) for 8)) was determined.

[0037]

[Table 1]

Specifically, the above bacteria were inoculated into a Mueller Hinton Broth liquid medium (manufactured by DIFCO) containing a sucrose fatty acid ester and propyl paraoxybenzoate mixed at the ratios shown in Table 1 by a multiple dilution method. 24 at 35 ° C
After culturing for a period of time, the obtained bacterial solution is then transferred to an SCD agar medium (So
Ybean-Casein Digest Agar (Nippon Pharmaceutical Co., Ltd.) was inoculated and cultured again at 37 ° C. for 24 hours. From the growth status of the fungus, the minimum concentration of the antibacterial composition in which the fungus did not grow (minimum bactericidal concentration, MBC) I asked. Axillary odor bacteria ( Corynebacter ium mi
nutissimum ) and the results for Staphylococcus aureus are shown in FIG. In the figure, the result of the experiment using propyl paraoxybenzoate alone is `` paraben alone '', and the result of the control experiment using each sucrose fatty acid ester alone is `` C12 alone '' according to the carbon number of the fatty acid, `` C14 alone, C16 alone or C18 alone (the same applies to the following figures).

Axillary odor bacteria ( Corynebacterium minutissimum )
For Corynebacterium spp., Etc., as shown in FIG. 1, by using sucrose fatty acid ester in combination with paraoxybenzoic acid ester, the amount of paraoxybenzoic acid ester, which may cause hypersensitivity, is impaired in its bactericidal effect. It was found that the bacterium can be remarkably reduced without using the bacterium, and the bacterium can be sterilized and removed with a small amount of use. As for Staphylococcus aureus, as shown in FIG. 2, it was found that the bactericidal action against Staphylococcus aureus was synergistically enhanced by using sucrose fatty acid ester and paraoxybenzoate in combination. On the other hand, no remarkable bactericidal effect was observed on Staphylococcus epidermidis (results not shown).

Experimental Example 2 Bacteriostatic action against various bacteria (MI
C) Similarly to Experimental Example 1, sucrose fatty acid ester (fatty acid having 12 to 18 carbon atoms) and paraoxybenzoic acid ester (propyl) were mixed at the ratio (weight ratio) shown in Table 1 above, and the mixture was used for testing. Used as an antimicrobial composition, various bacteria (armpit odor bacteria [ Corynebacterium minutissimum ] (IFO15361), Staphylococcus aureus [IFO13276], Staphylococcus epidermidis ] (ATCC1222)
8), Pseudomonas aeruginosa (IFO13275),
Bacillus subtilis (IFO3134), E. coli [ E
scherichea coli ] (IFO3972) <the bacterium>; Aspergillus (filamentous fungus) [ Aspergillus nigar ] (IFO945
5) Black fungus [ Cladosporium cladosporioides ] (IFO30
313), the minimum growth inhibitory concentration (MIC) for Candida albicans (IFO1594) (more than fungi) was determined.

Specifically, the bacteria were mixed with each of the antibacterial compositions described in Table 1 by a multiple dilution method to obtain Mueller H.
Inton Broth liquid medium (manufactured by DIFCO) was inoculated with each of the above bacteria and cultured at 35 ° C. for 24 hours, and the turbidity of the bacterial solution (630 nm)
Determined the minimum concentration (minimum growth inhibitory concentration, MIC) of the antibacterial composition that did not exceed the turbidity of the control medium (medium cultured in the same manner without cells). As for fungi, Sabouraud Liquid B containing each of the antibacterial compositions described in Table 1 was used.
Each of the above fungi was inoculated into a roth Modified Antibiotic Medium 13 (BBL) medium and cultured at 37 ° C., and the minimum concentration (minimum growth inhibitory concentration, MIC) of the antimicrobial composition showing no growth after 48 hours was determined. . As a control experiment, for each sucrose fatty acid ester alone and for propyl paraoxybenzoate alone, the minimum growth inhibitory concentration for each bacterium was measured in the same manner, and the effect of the combination of both was evaluated.

Axillary odor bacteria ( Corynebacterium minutissimum )
Fig. 3 shows the results of the reaction against Staphylococc.
The results for us aureus) in FIG. 4, Staphylococcus epidermidis (S
taphylococcus epidermidis ) is shown in FIG.
Fig. 6 shows the results for Pseudomonas aeruginosa .
In, Figure 7 the results for Bacillus subtilis (Bacillus subtilis), Figure 8 the results for E. coli (Escherichea coli), the result for Aspergillus (Aspergillus Nigar) in FIG. 9, black fungus (Cladosporium cladosporio
Figure 10 the results for ides), and Candida (Cand
ida albicans ) is shown in FIG. In each figure, the results of the control experiment using each sucrose fatty acid ester alone are `` C12 alone '', `` C14 alone '', `` C16 alone '' and `` C18 alone '' according to the carbon number of the fatty acid,
The result of the control experiment using propyl paraoxybenzoate alone is described as "paraben alone".

As can be seen from the results, the combined use of a sucrose fatty acid ester and a paraoxybenzoic acid ester gave rise to an axillary odor ( Corynebacterium minutissimum ).
It can be seen that the bacteriostatic action is synergistically enhanced against Corynebacterium sp. (FIG. 3), S. aureus (FIG. 4), and S. epidermidis (FIG. 5). Especially axillary odor bacteria ( Co
rynebacterium minutissimum ) showed bacteriostatic action at extremely low concentrations. Such synergistic enhancement of bacteriostatic action was selective for axillary odor, S. aureus and S. epidermidis.

Considering the results of Experimental Examples 1 and 2 together, the antimicrobial composition of the present invention shows a low concentration of axillary odor at a low concentration based on the synergistic effect of the combined use of sucrose fatty acid ester and paraoxybenzoate. It has excellent bactericidal activity against Corynebacterium spp., Such as bacteria, and is used as an antibacterial agent for Corynebacterium spp., Especially for axillary odor bacteria, as an external skin preparation for axillary odor, and an axillary odor inhibitor (anti-axillary odor agent) Is considered to be useful. Further, the antibacterial composition of the present invention has a bacteriostatic action against Staphylococcus epidermidis, which causes sweat odor, and its growth can be significantly suppressed. Therefore, it can be used to prevent the generation of sweat odor ( Deodorant (antiperspirant)
etc). Furthermore, the antibacterial composition of the present invention can significantly suppress the growth and proliferation of Staphylococcus aureus, and is therefore considered to be useful for preventing suppuration of wounds. Moreover, since the antibacterial composition of the present invention does not exert a bactericidal effect against Staphylococcus epidermidis even at a high concentration, it can maintain the skin's original self-cleaning function and barrier function without destroying the skin microflora. .

By the way, it is known that Staphylococcus aureus is detected at a high probability in the eruption area of patients with atopic dermatitis, and it is known that the number of existing bacteria is large. In atopic dermatitis patients, it is also considered useful to normalize the skin by promoting the maintenance and formation of healthy skin microflora. Since the antibacterial composition of the present invention selectively acts on Staphylococcus aureus without impairing the skin microflora as described above, it is also useful as a skin external preparation for patients with atopic dermatitis. .

It should be noted that other bacteria, especially Aspergillus,
Since the antibacterial effect of paraoxybenzoic acid ester combined with sucrose fatty acid ester is also recognized against fungi such as black fungi and Candida fungi (FIGS. 9 to 11), the combined use of sucrose fatty acid ester requires paraoxybenzoic acid. It was considered useful in that the amount of the paraoxybenzoate could be reduced without lowering the preservative effect of the acid ester.

Example 3 Odor Control Test 12 sweats were collected from 12 volunteers exercising in an environment at a temperature of 40 ° C. and a humidity of 75%. These sweats are divided into four equal parts (samples 1 to 4), sample 1 is left as it is (without adding an antimicrobial agent), and sample 2 contains the antimicrobial composition of the present invention (1000 ppm of sucrose palmitate + 1000 ppm of propyl paraoxybenzoate). ), 1000 ppm of sucrose palmitate was added to sample 3, and 1000 ppm of propyl paraoxybenzoate was added to sample 4 to obtain test samples 1 to 4 for the deodorization test. These test specimens 1 to 4
After shaking culture overnight, the odor of each culture was smelled by 11 panelists specialized in odor, and the deodorant effect of the antibacterial composition of the present invention was evaluated. The result is shown in FIG.

From these results, it can be seen that the use of each of sucrose fatty acid ester and paraoxybenzoate alone and the combined use of sucrose fatty acid ester and paraoxybenzoate significantly suppress generation of odor due to microbial degradation, and provide excellent deodorization. It turned out to be effective.

Example 1 Cream Sucrose palmitate 0.8% (Surf Hope SE COSME C-1616, Mitsubishi Chemical Foods) Sucrose myristate 3.0% (Surf Hope SE COSME C-1416, Mitsubishi Chemical Foods) ) Propyl parahydroxybenzoate 0.2% Stearic acid 13.2% Polyoxyethylene sorbitan monostearate 2.0% Propylene glycol 24.0% Glycerin 6.0% Sodium hydroxide 0.6% Purified water 50.2% 100.0% in total.

Example 2 Lotion squalene 2.0% glyceryl monostearate 1.2% stearic acid 0.8% cetanol 0.4% sucrose palmitate 1.0% (Surf Hope SE COSME C-1616, Mitsubishi Chemical foods) Propyl parahydroxybenzoate 0.2% 1,3-butylene glycol 3.0% Triethanolamine 0.2% Purified water 91.2% Total 100.0%.

Example 3 Pump spray Sucrose laurate 0.8% (Surf Hope SE COSME C-1216, Mitsubishi Chemical Foods) Butyl paraoxybenzoate 0.2% Ethanol 79.0% Isopropanol 20.0% Total 100.0%.

[0052]

The antimicrobial composition of the present invention has an excellent bactericidal and bacteriostatic action against Corynebacterium or Staphylococcus aureus. In particular, since the antibacterial composition of the present invention has an action of inhibiting the growth of the staphylococcus epidermidis, which is a resident bacterium, without sterilizing the bacterium, the skin barrier function due to the resident bacterium even when applied directly to the skin. It is possible to prevent generation of axillary odor or suppuration such as a wound without impairing the properties. Therefore, the antibacterial composition of the present invention is an antibacterial component of a skin-friendly external preparation for preventing axillary odor (for axillary odor), as an antibacterial component of a deodorant (antibody odorant, anti-axillary odorant),
It is also useful as an antibacterial component for preventing suppuration.

Since the external preparation for skin and the anti-axillary odor agent of the present invention contain the above-mentioned antibacterial agent as an antibacterial component, they have excellent selective antibacterial (bactericidal / bacteriostatic) effects against Corynebacterium and Staphylococcus aureus. It is a skin-friendly preparation that acts bacteriostatic against Staphylococcus epidermidis.

[Brief description of the drawings]

FIG. 1 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoic acid ester, which comprises an axillary bacterium ( Coryneba).
minimum bactericidal concentration (MB) for cterium minutissimum
C) Drawings showing the results of Experimental Example 1 in which the (bactericidal action) was examined.

FIG. 2 shows the minimum bactericidal concentration (M) of an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester against Staphylococcus aureus.
3 is a drawing showing the results of Experimental Example 1 in which BC) (bactericidal action) was examined.

FIG. 3 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoic acid ester, which is composed of an axillary bacterium ( Coryneba).
cterium minutissimum )
3 is a drawing showing the results of Experimental Example 2 in which IC) (bacteriostatic action) was examined.

FIG. 4 shows the minimum inhibitory concentration (MIC) (bacteriostatic action) of an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate on Staphylococcus aureus, which was examined in Experimental Example 2. It is a drawing showing a result.

FIG. 5 shows the minimum inhibitory concentration (MIC) (bacteriostatic action) of an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate on Staphylococcus epidermidis . It is a drawing showing a result.

FIG. 6 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoic acid ester, which is composed of Pseudomo.
nas aeruginosa ) (MIC)
4 is a drawing showing the results of Experimental Example 2 in which (bacteriostatic action) was examined.

FIG. 7 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester of Bacillus bacillus ( Baci).
llus subtilis )
4 is a drawing showing the results of Experimental Example 2 in which (bacteriostatic action) was examined.

FIG. 8 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester of Escherichia coli ( Escheric
5 is a drawing showing the results of Experimental Example 2 in which the minimum inhibitory concentration (MIC) (bacteriostatic action) against Hea coli was examined.

FIG. 9 shows the minimum inhibitory concentration (M) of an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester against Aspergillus nigar.
3 is a drawing showing the results of Experimental Example 2 in which IC) (bacteriostatic action) was examined.

FIG. 10 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester of a black fungus ( Clad).
3 is a drawing showing the results of Experimental Example 2 in which the minimum inhibitory concentration (MIC) (bacteriostatic action) against osporium cladosporioides was examined.

FIG. 11 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoate ester of Candida fungi ( Ca).
ndida albicans )
4 is a drawing showing the results of Experimental Example 2 in which (bacteriostatic action) was examined.

FIG. 12 shows an antibacterial composition containing a sucrose fatty acid ester and a paraoxybenzoic acid ester (test sample 2 in the figure)
3 is a drawing showing the results of Experimental Example 3 in which the deodorizing power of the sample was examined. In the figure, the specimen 1 has no antibacterial agent added, the specimen 2 has an antibacterial agent (1000 ppm sucrose palmitate + 1000 ppm propyl paraoxybenzoate), the specimen 3 has an antibacterial agent (1000 ppm sucrose palmitate), No. 4 is an antibacterial agent (propyl parahydroxybenzoate 1000 ppm) added.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 31/7016 A61K 31/7016 A61P 17/00 101 A61P 17/00 101 31/04 31/04 F term ( Reference) 4C083 AB032 AC012 AC072 AC092 AC102 AC122 AC242 AC422 AC442 AC471 AC472 AC542 AD221 AD222 BB48 CC04 CC05 CC17 DD08 DD23 DD31 EE18 4C086 AA01 AA02 EA03 MA02 MA04 MA63 NA05 ZA89 ZB35 4C206 AA01 AA02 DB17 MA02 MA04 MA04 BC03 DA21 DB05 DD06 DE16

Claims (7)

[Claims] An antibacterial composition comprising a sucrose ester of a fatty acid having 8 to 22 carbon atoms and a paraoxybenzoate or a salt thereof. 2. A bacterium of the genus Corynebacterium.
2. The antibacterial composition according to claim 1, which has an antibacterial effect on msp.). 3. The genus Corynebacterium.
m sp.) are antimicrobial composition of claim 2, wherein the bacteria causing body odor. (4) Staphylococcus aureu
2. The antibacterial composition according to claim 1, which has an antibacterial action against s ). 5. The antibacterial composition according to claim 1, which is used for skin. 6. An external preparation for skin containing the antibacterial composition according to any one of claims 1 to 4. 7. An anti-axillary deodorant comprising the antibacterial composition according to claim 3.