JP2002145732A - External preparation for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an external preparation for skin that has anti-inflammatory effect, collagenase activity-inhibitory effect, hyaluronidase activity-inhibitory effect, skin-whitening effect, humectant effect, antioxidant effect and the like. SOLUTION: The objective external preparation for skin includes an extract from seeds of a plant, longan (Euphoria longana) in Sapindaceae.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin characterized by containing an extract of longan seeds. The field of application is, for example, cosmetics (for humans and other animals). Various preparations to be used), pharmaceuticals, quasi-drugs and the like.

[0002]

2. Description of the Prior Art Euphoria longgan
a) is of the order Sapindacea (Sapindacea)
This plant belongs to e) and is widely cultivated as a fruit tree in tropical America and Southeast Asia. 1 cm inside the tan skin
Contains jelly-like pulp to wrap large seeds. This pulp is used as a raw or dried Chinese herb with a tonic or sedative effect, and is also used as a longan extract in cosmetics. However, the seeds remaining after the removal of the pulp are discarded, and there has been no example of evaluating the pharmacological effect or the cosmetic base. Accordingly, the present inventors have studied and pursued a unique and useful method of use and action of longan seed, and as a result of intensive research, it has been found that the longan seed extract has anti-inflammatory activity, collagenase activity inhibitory activity, hyaluronidase activity inhibition. It has an activity, a whitening action, a moisturizing action, and an antioxidant action, and has been found to be very effective to be applied to an external preparation for skin, and has completed the present invention.

[0003]

An object of the present invention is to provide a novel external preparation for skin containing an extract of longan seed.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above-mentioned object, and as a result, it has been found that an excellent anti-inflammatory action, collagenase activity inhibitory action, hyaluronidase activity inhibitory action, and whitening effect of longan seed extract are excellent. The present inventors have found that they have an action, a moisturizing action and an antioxidant action, and that they have high safety, and have completed the present invention.

The longan seed extract for use in the present invention can be prepared by extracting longan seeds with water, alcohol, polyhydric alcohol or a mixture thereof, and using them in solution. However, powdery products obtained by drying these solutions can also be used for cosmetics. In extracting longan seeds, a buffer solution, a dilute acid or a dilute alkali can be used instead of water.

It is desirable that the longan seed extract of the present invention be dried after drying the longan seed, and then sufficiently washed with water to extract the impurities. The longan seed from which impurities have been removed can be extracted as it is, but can also be pulverized before being subjected to extraction. Longan seed extract is obtained by washing the washed longan seed with water, methanol, ethanol, isopropanol, lauryl alcohol, cetanol, natural alcohols such as stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol, isostearyl alcohol. , Synthetic alcohols such as 2-octyldodecanol, ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether,
Diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, polyhydric alcohols such as mannitol, ketones such as acetone, diethyl ether, dioxane, Acetonitrile, ethyl acetate, isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate,
Esters such as cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, pyridine, chloroform, benzene, toluene, xylene, cyclohexane, Organic solvents such as hexane, avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange raffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil,
Tallow fatty acid, kuku nut oil, safflower oil, shea butter,
Soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, pig Fats such as fats or hydrogenated products of these fats and oils (hardened oils and the like), beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, waxes such as candelilla wax, montan wax, shellac wax, liquid paraffin, Vaseline,
Mineral oils such as paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, and tall oil , Natural fatty acids such as lanolin fatty acids, synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid, citrate buffer, phosphate buffer Buffer solution such as borate buffer, Tris-HCl buffer, diluted alkali such as dilute caustic soda, dilute caustic potash, sodium carbonate and ammonia, dilute acid such as dilute hydrochloric acid, dilute citric acid, dilute acetic acid, dilute lactic acid, or a mixture thereof. In addition, -30 to 110 ° C
For one hour to two months.

The extracted longan seed extract can be used as it is, but it is preferable to further purify it by filtration, centrifugation or the like. In many cases, it can be used as is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected.
An activated carbon column or the like may be used as a purification treatment means such as deodorization and decolorization, and any ordinary means generally applied to the extracted substance may be selected. Further, the obtained Longan seed extract can be obtained as a dried product by a heat drying method such as a freeze drying method and a spray drying method.

[0008] Cosmetics to which the longan seed extract of the present invention is blended include face lotions, lotions, serums, general creams, cleansing creams, and the like, packs, shaving creams, tanning creams, sunscreens, and the like. For sunscreen lotion, suntan lotion, toilet soap, foundation, whitening, powder, lipstick, lip balm, eyeliner, eye cream, eyeshadow, mascara, bath cosmetics, shampoo, rinse, hair dye, hair cosmetics, etc. Available. The content thereof can be 0.001 to 100% in the cosmetic composition, but is preferably 0.001 to 10%, and particularly preferably 0.01 to 5%.

The external preparation for skin of the present invention may be arbitrarily selected from the various components and additives usually used in external preparations for skin such as cosmetics as described below, and those which are suitable for the intended use may be used in combination. Can be manufactured.

[0010] For example, avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil,
Safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro , Coconut oil, beef tallow, lard, and the like, or hydrogenated products of these fats and oils (hardened oil, etc.).

Waxes such as beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax and the like. Mineral oils such as liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, and pristane. Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid,
Natural fatty acids such as undecylenic acid, tall oil, and lanolin fatty acids.

[0012] isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-
Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.
Ethanol, isopropanol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol and phytosterol. Synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol and 2-octyldodecanol.

[0013] Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3 Polyhydric alcohols such as butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol;

Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl phthalate, phthalate Esters such as diethyl, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.

Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate,
Metal soaps such as zinc laurate and zinc undecylenate.

Gum arabic, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin , Starch, carrageenan, succinyl chitosan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan added with alkylene (C2-C4) oxide such as ethylene oxide, low molecular weight chitin or chitosan, chitosan salt,
Sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate and its salts, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxyethylcellulose sodium, hydroxyethylcellulose, hydroxypropyl Cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, polyethyleneimine, etc. Gums, sugars and water-soluble polymer compounds

Anionic surfactants (alkyl carboxylate, alkyl sulfonate, alkyl sulfate,
Alkyl phosphate salts), cationic surfactants (alkyl amine salts, alkyl quaternary ammonium salts), amphoteric surfactants: carboxylic acid type amphoteric surfactants (amino type,
Betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, ether ester type nonionic surfactant) Agents, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants, protein hydrolyzate derivatives, polymer interfaces) Surfactants such as surfactants, surfactants containing titanium and silicon, and fluorocarbon surfactants).

Retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folate , Nicotinic acids, pantothenic acids, biotins, choline, inositols,
Ascorbic acid and its derivatives, ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxerol, tocopherol and its derivatives, ubiquinones, phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3) ), Menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamins such as vitamin U.

Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, Ornithine,
Amino acids such as histidine and their sulfates, phosphates, nitrates and citrates; and amino acid derivatives such as pyrrolidone carboxylic acid.

Microbial culture metabolites such as yeast metabolites, yeast extract, rice ferment extract, rice bran extract, euglena extract, trehalose or derivatives thereof. Α-hydroxy acids such as glycolic acid, citric acid, malic acid, tartaric acid and lactic acid.

Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, Inorganic pigments such as black iron oxide, gunjou, chromium oxide, chromium hydroxide, carbon black, and calamine.

P-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, UV absorption of nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin, etc. A blocking agent.

P-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin,
Whitening agents such as baicalin, baicalein, and berberine.

Ascorbic acid and its derivatives, hydroquinone and its glycosides, kojic acid and its derivatives, placenta extract, silk peptide, plant extracts (mulberry, corn,
Waremokou, mugwort, honeysuckle, yellowfin, prickle,
Matsuhod, Adlay, Pompidium, Hawthorn, Eucalyptus, Achillea millefolium, Altea, Keihi, Mankeishi, Hamamelis, Yamaguwa, Extended life grass, Bellflower, Toshishi,
Zuisonko, Sun-dried, Mao, Senkyu, Dokkatsu, Psycho,
Boufu, Hamabofu, Ogon, Peony, Gennoshoco, Licorice, Fivefold child, Aloe, Ginger, Safflower,
Tyrosinase activity inhibitors such as Asenyaku.

A melanin pigment reducing / decomposing agent such as hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, aqueous hydrogen peroxide and zinc peroxide. Hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, ogon, horsetail, gentian, burdock, sicon, carrot, Turnover-promoting / cell activators, such as hamamelis, hops, yokuinin, edulis, assembly, touki, balsam, amacha, hypericum, cucumber, scorpion, rosemary and parsley.

Astringents such as succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, and tannic acid (including catechin compounds). Active oxygen scavengers such as SOD, catalase and glutathione peroxidase.

Ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA),
Antioxidants such as hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin and gossypol.

Β-carotene, plant extract (cultured sesame cells, amacha, hypericum, hamamelis, clove,
Lipid peroxide production inhibitors such as melissa, emicou, birch, sage, rosemary, Minami Tenmi, kina, age, ginkgo). Iktamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, Anti-inflammatory agents such as licorice extract component, sicon extract and age extract.

Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyldiaminoethylglycine chloride solution, isopropylmethylphenol, Disinfectants for disinfectants such as triclosan and kujin.

Glycerin, propylene glycol, 1,
Humectants such as 3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, pyrrolidone carboxylic acid and its salts, sodium lactate, and mini-sasanashiki extract.

Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thiantole, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thioglycolic acid, etc. For hair.

Antiandrogens such as estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone and the like. Vitamin E and its derivatives, assembly extract, garlic extract,
Peripheral vascular blood flow enhancers such as ginseng extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride and minoxidil.

Topical stimulants such as pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor and benzyl nicotinate. Metabolic activators such as photosensitizer 301, hinokitiol, pantothenic acid and derivatives thereof, allantoin, placental extract, biotin, and glyceride pentadecanoate.

Antiseborrheic agents such as pyridoxine and its derivatives, sulfur and vitamin B6. Resorcinol, salicylic acid,
Keratolytic agents such as lactic acid. Aqueous hydrogen peroxide, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, sodium orthophosphate And oxidizing agents such as sodium hydrogen silicate peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, and mushroom extract.

Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide and calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, thioglycolate) Hair remover such as strontium acid).

Hair swelling agents such as ethanolamine, urea and guanidine. 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5
-Dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium piclamate, N ', N-bis (4-aminophenyl)-
2,5-diamino-1, -4-quinonediimine, 5-
(2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, Orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, Paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-na Tall, hydroquinone, pyrogallol, fluoroglycine log arsine, gallic acid, resorcinol, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diamino azo benzo Zen-5'-sodium sulfonate,
Dyes such as hematein.

Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil,
Cardamom essential oil, guaiac wood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, cedar wood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Greater Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batchy essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil,
Plant flavors such as rosemary essential oil and Japanese peppermint essential oil,
Other fragrances such as synthetic fragrances.

Red cabbage pigment, red rice pigment, madder pigment,
Anato color, squid color, turmeric color, orange color, krill color, persimmon color, caramel, gold, silver, gardenia color, corn color, onion color, tamarind color,
Pigments and coloring agents such as spirulina pigment, buckwheat whole plant pigment, cherry pigment, nori pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment and rutin.

Also, almonds (red peaches), eyes (blue leaves), blue vegetation (Seifu Fuji), blue vegetation (Kibo), blue-green agave, red-green agave, red grape, red grape, red-green ape Red buds, Akane (Akane grass roots), Akajiojiu Jiou (Jihuang), Agi (Awei), Akinire (Royu skin), Akebi (Kidori), Asa (Asakojin), Malva asagao or Asagao (Toshiko), Ashitaba (Asuka), Azuki (red azuki), Acerola, Asenyaku (Asenyaku), Anise, Abemaki fruit, Avocado,
Ama, Amacha (Amacha), Amachaworu, Amadokoro (Tamatake), Amigasa lily (Shellfish), Altea, Arnica, Aloe (Lugu), Aloe vera, Angelica, Anzu Honganzu (Anjin), Ansokkou (Azuka), Igakozolina (Earth) Bile head), knotweed (tiger root), strawberry, fig (apricot) or its leaves, strawberry (winter aoiko), ichiyakusou, ginkgo (ginko leaves, ginkgo), Itohimehagi (enshi), carob, rice seed or seed coat,
Inondo seeds, Ibuki gypsophila, nettle, ylang-ylang, fennel (fenka), ukiyagara (sanryo), guisukagura fruit, himeuikyo, turmeric (depression),
Usubasaishin Keirinsaishin (spicy), Usbenioioi, Usbeniitaoioi, Utsubogusa (summer hay),
Udo or Shishudo (Qanggai, Independent, Tang Deng), Plum (Ume) or its pulp, Vladimir, Oolong tea, Uwaurushi (Kusaco peach), Unshu mandarin (Chen skin),
Estragon, Ezokogi (Ezo Goka), Ezoki strawberry, Echinashi (Hosobamurasakibarengiku), Enishida, Enokitake (Enokitake), Ebisugusa (Keiko), Elderberry fruit, Elemi, Eringyi (pre-lotus erinji), Enduyu (pre-flower) (Rice blossom), ogi / kibanaogi (radix astragali), uren (orange), giant crow squirrel (karonin), ootsu-la-fuji (self-defense), psyllium (vegetative child, vegetative grass), tou-habami (haruko), eucalyptus okaera (white jujitsu) ), Obanaya shabushi fruit, O hawthorn hawthorn (Yamako), Oohisasanoyuki, Sasanoyuki, Barley (barley), Ogurma (turning), Okra fruit, Panax ginseng Tochibanjin (Ginseng), Ogirisou Kogomebayotoyosougi grass Odrico C (continued cross-sectional), cocklebur (Aoimimiko), Juglans, Gastrodia elata (Tian Ma), Tiger -
Sasayuri Hakatayuri (Yuri), Halibut (Hihayu),
Ominaeshi (defeated soybean), Dutch strawberry, Dutch mustard (Watercress), Dutch jelly, Dutch honeysuckle, olive, oregano, orange fruit or pericarp, silkworm (ripened yellow), cacao seed, oyster or its leaves (persimmon), (Katsuo Choto), Cassia, Cassinaki fruit (Kozou), Gajutsu (Shazajutsu), Kashiwa (Shakiju, Sakuraha),
Cascarilla, Kanixa (Kinosato), Valerian (Kisosone), Birch or Weeping birch (White birch), Pumpkin, Capoknoki seed, Kahoku Sansho (Shupper), Gama (Gambah), Chamomile roman chamomile, Kamiyatsude (Tsou grass), Kam Kam / Duck, duckweed (gold melon),
Crow bishaku (midsummer), oat wheat, guarana seeds, karahoo (thick), karaya, karin (mochi), garcinia, kawayanagi, kawaratake, kawamuragi (inchin bulk), kansui (successful), licorice (licorice), kanaraasa, Candelilla, canto, canna, raspberry, kiwi fruit, kikarasuuri (urorone), kikyo (bellflower, bellflower), chrysanthemum (chrysanthemum), catalpa (azumi), sagebrush (yotei), pheasant (komi), kizuta , Kidachi aloe,
Yellow mint, quince, red linden (shincona, red linden), yellow fin (yellow), gymnema sylvestre, cabbage, cabbage immature fruit, cucumber, Kiraja saponaria, kyalaya, kumquat fruit, betel, beetroot, guava fruit, guayule, Quercus Infectria (Gallery), Kukui Nuts, Wolfberry (Gugi, Gyugia, Gyugi Leaves, Skeletal Bark), Quercus spp. (Tenmen Winter),
Camphor tree, gooseberry fruit, gardenia, gardenia, kunugi (bokusou), kumazasa, black pine (horse whip), clara (bitan), cranberry fruit, chestnut or its skin, curculigo latifolia fruit, grapefruit, buckthorn, black squirrel Rescue), Kurobana Hiokikoshi, Clove (cloves, cloves), Kaigai (codling, codling spikes), Celosia (corolla, cotyledon), bay laurel (laurel), kenashisarutoribara (tokubukuryo, mountain return), gentian, gennoshoko (old) Kouzi / Tachibana / Obenimikan / Ponkan / Santara (Tachibana skin), Koushinbara (Monthly flower), Kouzui mint, Kozo fruit, Koucha (tea), Kouhone (river bone), Koufon (straw book, Kara straw book), Koulyang, cilantro fruit, koganebana (yellow gon), Chemomo (Koshibachi), coconut fruit, Goshuyu (Goshuyu), Goshostrawberry (covered bonko), pepper (pepper), copaiba balsam, coffee beans, Kobushi magnolia (spicy), burdock (burdock, burdock), kobotur, sesame (Sesame), sesame seedlings (ginseng), garnishes (gomiko), Sanekazura binankazura Matsubusa,
Wheat (wheat), rice, rice bran (red bran, white bran), rice oil, cola acuminata seeds, cola vera seeds, fenugreek fruit, colombo, conzulango, kelp, konjac, comfrey (finnari grass), sisal, sisal asa , Sakisima buttonpul (weird sen), sakura (Oshimazakura, Yamazakura, Oyamazakura, Edhigashi, Mamezakura,
Leaves, flowers, fruits, bark (cherry bark), cherries, pomegranate, sasa, sasanqua, sardine, sweet potato, sugar cane, sugar beet, Jujube jujube (acid jujube), saffron (banana safflower, western safflower), pomelo fruit, sabonso, salmon berry fruit, sarashinasho (masuma), salvia (sage), sawagikyo (yamachona), sawagurumi (yamawomo), sanshichi Carrot (37 ginseng), sanshuyu (sanshuyu), sansho (sansho),
Sandscon (Yamazone), Shea (Karite), Cyanoki fruit, Shiitake (Shiitake), Shion (Shion), Dikitaris,
Sikunshi (Kimiko), Shiso Aojiso Chirimenjiso
Katamenjiso (perilla leaves, perilla), rosewood, linden,
Pterodactyla, Lynchium japonicus (serial forsythia), Shimakangiku (chrysanthemum flower), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiiroshimeji, Bunashimeji, Shirotamogitake), Shimotsukesou, Potato, Paeonia ), Jujudama, Palm fruit, Ginger (ginger), Shobu (Iris, Iris root), Shozuku fruit, Shirakashi seed, Shirogoshi butterfly seed, Shirobanatsuta flower, Shirominanten (Minami Tenmi), Cinchona saxilbra, Cinnamon, Watermelon ( Watermelon), honeysuckle (gold and silver flowers, Shinobi winter), sorrel (acid model),
Swimberry fruit, Stevia, Strawberry fruit, Horsetail (Thorn), Stevia, Plum fruit, Scots pine cone, Larch of Europe, Scotch vine, Scotch pine walnut, Atlantic hawthorn, European dandelion, Horse chestnut (Maloney), Atlantic pear fruit, Atlantic snowfruit, Western elderberry (Elder), Atlantic shrew (Juniper / Morimatsu), Achillea millefolium (Milfoil), Atlantic rose, European cornflower, European mistletoe, Atlantic lipstick, Japanese horseradish, Japanese horseradish , Hirohasenega, senega, seri, celery, senkyu (river kyu), sendan, senburi (drug), senna fruit or leaves, senninsou (large tate), souka (grass), buckwheat seeds, sui Monoimo, rhubarb (daioh), radish, soybean, rhubarb (orange, kikomi), takatodai (daigo), takawarabi (dog sparrow), dark sweet cherry fruit, hollyhock, scotch thyme (thyme, Hyakurika), shiroyanagi, tamarind seeds , Onion, tamshiba (hot spring), arachis or its root bark, tanjin (dansin), dandelion (gakkohide) or shirobana dandelion, moko dandelion, dammar, cherry fruit, tigaya or its root (chinese ginseng), chikusetsujin (bamboo ginseng) , Chicory, ginseng (Gomiko), ginseng (daiko), ginseng (Wuyi), ginseng (carrot), ginseng (Ginseng), artemisia (Aoba), choreimaitake (Irei), Tsukimiso (mushroom), camellia , Spiderweeds, clovers , Tsuyukusa (Dark primrose), Tsuruazuki (red azuki bean), Tsurudukami (Hanshu crow), Tsuruna (Banju), Tsuruninjin (Four leaf ginseng), Tsuwabuki, Deiko, Teuchigurumi, Duberry fruit, Tengusa, Tencha, Tendaiyaku ), Pepper (Furry), Pepper (Pepper), Touki (Toki), Calendula (Marigold), Maize or Maize Hair (Nanban Hair), Toad Mizuchi (Female Sadako), Torindo (Dragon Bile), Dokudami (10 medicines), Tokon (sore root), Toshishi, Mamedaoshi, Nashikazura, Eucommia (Tonaka, Tonaka leaf), Tomato, Traganto, Triacanthus seed, Tormentilla, Dronoki, Troloaoi, Niseria berry fruit, Yam yam (Yamayaku) , Nagikadada (Butcher Bloom), Naginata Kouju, Na Zuna, rapeseed, jujube, jujube (daijuju), nanawaibara (gold cherry blossom), nameko, narco lily (yellow), peanut (peanut), nanten (minamitenmi), nigaki (makinoki), nigarimogi (bitumen), nikuzuku, Fruits, flowers or foliage of Kei Nikkei (Kihi bark) / Keishi (Keishi), Chinese chive (Nirako), Elderberry (bone-bonded tree),
Garlic (garlic), nurde (five times), green onion, thistle (daiji), wild rose (fruit), sawwood, sawdust (maehu), nobara, peach, palm, pineapple fruit, hibiscus (bussouge, hurinbussouge,
Roselle, Hakayamauraboshi (Bone Crushing), Hakusen (Rinosaur), Huckleberry Fruit, Hakobe (Regular), Hazel (Haruko), Hashiridoro (Roto Root), Basil, Lotus (Lotus, Lotus, Lotus), Parsley ( Dutch jelly), naked mussels, batata, hachiku madake (bamboo-boiled), patchouli,
Mentha (light load, light leaves), Adlay (Yokuinin), Hanasuge (Chimo), banana, Hanamint, vanilla bins,
Papaya, hakogusa (rat koji), hub grass, paprika,
Hamago / Mitsubahamago (vine thorn), Hamasuge (Katsukishi), Hamabishi (Situriko), Hamanas (maikai flower), Hamaboufu (beach storm), Hamamelis, rose (rose), agarita (haratake, white-faced agaricus) , Parietaria, Harunire (yu bark, yu white bark, yuba), breadfruit, lizard carrot (party),
Pecan nuts, Amaryllidaceae (garlic, vinegar), Hiokikoshi (extended life grass), Hishi (Ryomi), Pistachio, Beetroot horn (stone reed), Hinata Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himegama (incense blossom), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta fruit, Juniper, Bjakuyu, Hiyu fruit, Belowudoi, Hirohakinagusa (Hakuto Okina), Loquat (Loquat, Loquat leaf), Betelrow (Large husk, Betelnut) ), Sperm Kazura (Southern Rattan), Butterfly, Butterfly Dandelion (Winter Flower,
Subsidiary winter leaf), Fujibakama (orchid grass), Fuji bean (flat bean), grape fruit or pericarp, seeds and leaves, beech, Fuyumushinatsukutaketake (corticoleum), Brazilian liquorice, black currant fruit, blackberry, plum fruit, furcellaria, blueberry (Prunus avium), prunes,
Blond psyllium, bundo (green bean), loofah, safflower (safflower), henneken, belladonna, berry fruit, Peruvian berg, boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki (wakopoku, park) , Bokeh (Gourd Gourd), Hosovanaokera (Sojutsu), Hosobabarengiku, Bodaiju (Bodaiju), Button (Peonies, Peony Skin), Jojoba, Whiteberry Fruit,
Hon-shimeji, Maizulten Nanshaw (Tiannan star), Maitake (Maitake), Ephedra (mao), Macadamia nuts, Macri (Martian grass), Magwa (Mulberry white bark, Mulberry leaf), Magnolia splengeri, Matatabi (Mokuten tate) , Matsukasa, pine needles, matsuhodo (bukuryo), marjoram (hanahakka), malvanozadine (bitter), mulberry fruit, quince, mango, mangosteen, manshu gurumi, mandarin fruit, mannentake (reishiba), mandarin plant fruit (komi), Mishima Psycho (Saiko), Mizo Kakshi (Haren Lotus),
Misohagi (Chisatsuna), Mitsugashiwa, Mitsuba, Midorihakka, Mimosa, Myoga, Miracle Fruit Fruit, Myrrh, Milobaran, Mukuge (Tsuki), Mukunoki, Mokuroji, Murasaki (Purple Root), Murasaki Maize, Mehajiki (Merukibuki), Mebagi , Melissa, melilot, melon fruit, sagebrush, sagebrush, mushroom (wood incense), maple blossom, peach leaf (peach leaf)
・ Fruits and seeds (peach seeds), bean sprouts, morello cherry fruits, moroheiya (burlap), yakawamurasakiimo, yakuchi (masuti), cornflowers (cornflowers), cornflower mint, yashabushi (arrow wheels), yachiyanagi, yatsude (octagon metal plate) ), Mistletoe (willow parasitism), willow leaf, leaf syrup, yabukouji (purple gold beef), pokeweed (commercial land), yamahannoki (yamaharu), yamamomo (yangbai skin), yamamogi,
Eucalyptus, Saxifraga (tiger eargrass), Yucca flevifolia, Yuzu fruit, lily, yogurusa, European strawberry, mugwort (Aiba), lime fruit, rye, peanut seed coat, lakanka fruit, raspberry leaf and fruit, lavender,
Ryokucha (green tea), apple fruit, gentian, rubus
Suavisimus (sweet bean), lettuce, red currant fruit, lemon fruit, lemongrass, forsythia,
Plants such as astragalus, wax palm, locon (reed: root) loganberry fruit, rosemary (mannenrow), rosehip (nobara), wasabi, waremokou (jiyu), chlorella vulgaris, chlorella pyrenoidosa,
Green algae such as Chlorella ellipsoidia, Aonori (Usubaaonori, Sujiaonori, Hiraaonori, Bowaonori, Hosoedaaonori), and kelp (macombu, ricilisbu, hosomecombu, Mitsishicombu), wakame, hirome, aowakapsis macro Macrocystis integrifolia, Neocitys luetokeana), brown algae such as Hijiki and Hibamata, and Hijirimen, Maxa (Tengusa), Hiracusa, Onyxa, Obakusa, Cataobakusa, Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Tokakirinsai, Giraffe, juniper ginseng, tsunomata, obatsunomata, tochaka (yahatsunonomata), ezotunomata, togetsunomata, hirakotoji, koto Chondrus, Suginori, Shikin'nori, Kainori, Ibotsunomata, Yareusubanori,
Seaweed and Chlamydomonas represented by red algae such as Kagiusbanori, Sujiusbanori, Hyusbanori, Akamomininori, Chlamydomonas, Akayukimo, Dunaliella, Chlorococcus, Kwanomimo, Ohashigewari, Volvox, Palmera,
Hizaori, Amidro, Tsurugimidro, Hibimidoro, Anaaosa, Amiosaa, Nagaaosa, Kawanori, Fricciella, Ooishiogusa, Asamidoriushiogusa, Kawashiogusa, Marimo, Tamagovaronia, Tamabalonia, Magatatama, Fusaiwatamil, Husaiwatamil, Husaiwatamila , Nagamir, Hiramil, Casanori, Futojuzumo, Tamazuzumo, Mizojuzumo, Mikazukimo, Colecaete, Tsuzumimo, Kikkogusa, Hitoegusa, Hirohanohiegusa,
Green algae such as Usugusa, Motsukihitoe, Sayamidoro, Crimson ginger, Sumiremo, Hoshimidoro, Fushinashimidoro, etc. Senkurogashira, kashirazaki,
Muchimo, Hiramuchimo, Kebelligsa, Amidixa, Sakibiroamiji, Sanadagusa, Fukurinamiji, Common gusa,
Ezoyahazu, Yahuzugusa, Urabashiyahazu, Jigamigusa, Umiuchiwa, Konaumiuchiwa, Akabaumiuchiwa,
Hirnamimakukura, Somewagekusa, Nabarimo, Motikachasoumen, Matsumo, Nagamatsumo, Okinawamozuku, Niseftmozuku, Futomozuku, Ishimozuku, Kuromo, Nisemozuku, Mozuku, Ishige, Iroro, Ichimegasa, Keari, Umibosugoshi, Sasa, Samurai , Common hazel, Common bukuro, Ezobukuro, Fukuronori, Cottontail, Chishimafukuronori, Kagomenori, Murachidori, Shark's squirrel, Iwahige, Yokozimanori, Kayamamonori, Fukuoka, Tsurumo, Aname, Suzume, Misuzujikakonbu, Atsugakubunakonbukonkonbu , Pea kelp, Ootumi kelp, Toro kelp, Antokume, Kajime, Tsuruarame, Black Me, Kikuishi-kombu, Nejire-kombu, Kuroshiome, Nekoashi-kombu, Alame, Ainu-Wakame, Chigaiso, Ezoishige, Yabanemok, Rapamoku, Uganomok, Jiromoku, Hiemoku, Tamanashimoku, Isomoku, Nagashimamaku, Akamomaku, Shimomagawa, Shidamaramohon, Shidamo-makumo, Shimoda-mo-mo , Brown algae such as cedar, sea squirrel, squirrel, squirrel, squirrel, squirrel , Akebono Mozuku, Haikonahada, Yogorekonahada, Aokonahada, Umizomen, Tsukumonori, Ka Gashiranori, Benimozuku, Hosobenimozuku, Kasamatsu, Fusanori, Nisefusanori, Sodegarami, rattle, Hiragaragara, Hiro Ha Tama top, Tamaitadaki, Kaginori, Kagikenori, Himetengusa, high Gelidium, Oobusa, Nanbugusa, Kohira, Yohira, Kinukusa, Hibiroudo, Himehibiroudo, Isomumemodoki, Michigaesou,
Ryumonsou, Hera Ryumon, Nisekarekigusa, Oobaokitsubara, Aqaba, Marubaakaba, Hosovanaminominana, Naminohana, Garagamodoki, Shiogosagoromo,
Etsuki Iwanokawa, Kinokawa, Kaninote, Coral,
Centipede, Sujikade, Katanori, Hiramukade, Kyounoyomo, Sakuranori, Nikumukade, Tambanori, Tsururu, Nurakusa, Kronulakusa, Omukadenori, Hirakintoki, Matabou, Chabokintoki, Kintoki, Matsunori, Komatsunotori, Komatsunotori, Komatsunotori, Tonenomito , Fukuro nonori, mah nori, karekungusa, boninosato,
Hiro notosakamaki, yatsudegatatosakamoki, kurotosakamoki, nezashinosakamoki, kinuhada, ezotosaka, enashikarimenia, ootsukasanori, hanagatakakarimenia, honoo, hikakenoito, usuginorin, nikusuginorin, nikusuginorin, nikusuginorin , Ezonameshi, Isomokka, Yukari, Hosoyukari, Ibaranori, Saidaibara, Tachiibara, Kagiibaranori, Kijinoo, Isodanthus, Atsubanori, Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizookonori, Kabanori, Hachijougonosa, Hachijougonokasumi Saimi, Harigane, Lotus jigsaw, Ikanoashi, Hosoibonori, Nobonori, Kurohaginnanso, Red Gin'nansou, Hishibukuro, Togemadara, Etsukimadara, Taoyagisou, Hanasakura, Fukurotsunagi, Sujikonori, Hananoeda, Hirataoyagi,
Darus, Masagoshibari, Anadarus, Webagsa, Benifukuronori, Fushitsunagi, Himefushinagi, Hirohafushinagi, Hirawatsunagisou, Usuwawatsunagisou, Igis, Keigis, Hariigis, Hanagigis, Amixa, Egonomiri, Suzunikomi Hasujiginu, Nagakonohanori, Sujiginu, Atubasujiginu, Kagiusbanori, Yaleusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki, Ayanishiki, Ayaginu,
Enashidadia, Isohagi, Shimadagi, Dazimomodoki,
Moretogusa, Futogusa, Makuri, Hanayanagi, Yuna, Sanaginori, Motsureuna, Benyanagicoli, Mosanagi, Sasabayanaginori, Kurosozo, Kobusozo, Hanesozo, Sozonohana, Hanegusa, Kehanegusa, Kozanemone, Somuragoe, Somuragoe
Kikuhiodori, Hiodosigusa, Usubahioshi, Isomegusa, Suginashigusa, Isobasho, Fujimatsumo, Hakezakinokogirihiba, Kawamozuku, Aokawamozuku, Himekamozuku, Ideyugogome, Okuchimozuku, Chinomomida Axle algae such as chaboflask and tripella, and other algae such as yellow algae such as Hikarimo and cockscomb extract, placenta extract of cattle and human, extract of stomach and duodenum or intestine of pig and cattle or Decomposed products, extracts of swine or cow spleen or decomposed products thereof, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates ,Water soluble Lastin derivatives, keratins and their degradation products or their derivatives, silk proteins and their degradation products or their derivatives, porcine and bovine blood cell protein degradation products (globin peptides), and porcine and bovine hemoglobin degradation products (hemin, hematin, heme, Materials derived from animal sources such as protoheme, heme iron), milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, and fish meat decomposition products The processing is carried out in a conventional manner according to the product type and form to be added (eg, pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, extraction,
Fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., are arbitrarily selected and combined), and may be arbitrarily selected from various materials. .
The solvent used for the extraction may be selected in consideration of the processing to be performed later, but usually, water or a water-soluble organic solvent (eg, ethanol, propylene glycol, 1,3-butylene glycol, etc.) It is desirable to use one kind or a mixture of two or more kinds selected from the above.
However, when the content of the organic solvent is not preferable depending on the use, water alone may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary liquid mixture with water.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, fresheners, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral agents, deodorants / deodorants, bitters, enzymes, and the like are listed, and when used in combination therewith, various additive and synergistic effects can be expected.

[0041]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to the examples.

Production Example 1 Dried longan seeds were pulverized, and 10 g of the seeds were immersed in 100 mL of water and extracted at 60 ° C. for 24 hours. The precipitate was removed by centrifugation (10000 G, 10 minutes) to obtain a light brown clear longan seed extract.

Preparation Example 2 The longan seed extract obtained in Preparation Example 1 was freeze-dried in vacuum to obtain a freeze-dried powder of the longan seed extract.

Production Example 3 Dried longan seeds were pulverized, and 10 g were immersed in 100 mL of a 50% aqueous ethanol solution and extracted at 60 ° C. for 24 hours.
The precipitate was removed by centrifugation (10000 G, 10 minutes) to obtain a light brown clear longan seed extract.

Production Example 4 A dried longan seed was pulverized and 10 g of ethanol
and extracted at 60 ° C. for 24 hours. The precipitate was removed by centrifugation (10000 G, 10 minutes) to obtain a light brown clear longan seed extract.

Production Example 5 Dried longan seeds were pulverized, immersed in 10 g of a 50% 1,3-butylene glycol aqueous solution (100 mL), and extracted at room temperature for 2 weeks. The precipitate is centrifuged (10,000 G, 10
Min.) To obtain a light brown clear longan seed extract.

Production Example 6 Dried longan seeds were pulverized, and 10 g of the dried longan seeds were immersed in 100 mL of 1,3-butylene glycol and extracted at 60 ° C. for 24 hours. The precipitate was centrifuged (10000 G, 10 minutes), and then filtered to obtain a light brown clear longan seed extract.

Example 1 <Collagenase activity inhibitory action> The freeze-dried powder of the longan seed extract prepared in Production Example 2 was mixed with water at 1000 μg / m 2.
L, 100 μg / mL and 10 μg / mL were prepared as test solutions. Collagenase activity inhibition was measured using Collageno Kit CLN-100 manufactured by Collagen Technology Workshop. 50 μL of the test solution and 200 μL of human skin fibroblast-derived collagenase solution (0.1 units / mL) were added to 200 μL of the fluorescence-labeled substrate collagen solution, and 35
After reacting at 2 ° C. for 2 hours, the enzyme reaction was stopped with a reaction stop solution, and the decomposed product was extracted into an ethanol solution.
nm (Em) / 495 nm (Ex) fluorescence intensity (FI
[S]) was measured. The collagenase activity inhibition rate was calculated using the following equation.

(Equation 1)

FI [C]: Fluorescence intensity of a solution that was similarly operated using a buffer instead of a test solution FI [B]: A solution that was similarly operated using a buffer instead of an enzyme solution Fluorescence intensity

(Test Results) The results are shown in FIG. As is clear from FIG. 1, a high collagenase inhibitory activity was observed in the longan seed extract. The collagenase inhibitor is also used as a therapeutic drug for inflammatory diseases, indicating that it also has a strong anti-inflammatory effect on the longan seed extract.

Example 2 <Hyaluronidase Inhibiting Activity> The freeze-dried powder of the Longan seed extract prepared in Production Example 2 was purified using acetate buffer.
%, 0.1% and 0.01% to prepare test solutions. Hyaluronidase solution (330 units / mL) 0.1
Add 0.2 mL of each 1% and 0.1% solution to the mL and add 37 mL
Incubated at 20 ° C for 20 minutes. Further, 0.2 mL of an enzyme activity solution (2.5 mM calcium chloride.2H ₂ O)
And incubated at 37 ° C. for 20 minutes.
0.5 mL of a 08% hyaluronic acid solution was added, and the temperature was kept constant for exactly 40 minutes. Enzyme activity is Morgan-Els
It was measured by the on method. That is, 0.4 N
After 0.1 mL of NaOH was added to stop the reaction, 0.1 mL of a potassium borate solution was added, and the mixture was heated in a boiling water bath for 3 minutes. After cooling with running water, 3 mL of p-dimethylaminobenzaldehyde solution was added, and the mixture was stirred well and reacted at 37 ° C. for exactly 20 minutes. Absorbance at 585 nm (OD
[S]) was measured. The hyaluronidase activity inhibition rate was calculated using the following equation.

(Equation 2)

OD [C]: Absorbance of a solution similarly operated using a buffer instead of a test solution OD [B]: Absorbance of a solution similarly operated using a buffer instead of an enzyme solution Absorbance OD [T]: Absorbance of a solution that was similarly operated using a buffer instead of a hyaluronic acid solution

(Test Results) The results are shown in FIG. As is clear from FIG. 2, strong hyaluronidase inhibitory activity was observed in the Longan seed extract. Hyaluronidase, which is activated during inflammation as a result of a series of inflammatory reactions, is thought to play a role in destroying the matrix of connective tissue and increasing the permeability of inflammatory cells, thus significantly inhibiting this enzyme. It can be seen that the longan seed extract having a strong effect has a strong anti-inflammatory effect.

Example 3 <Whitening test> One cell culture bottle having a surface area of 25 cm ²
10 mL of DMEM medium containing 0% FBS, B16 cell suspension (2.5 × 10 ⁴ cells / mL, D containing 10% FBS
(MEM medium) 1 mL, CO ₂ concentration 5%, 37 ° C
For 24 hours. The solute concentration of the longan seed extract prepared in Production Example 1 was 0.5, 0.1, 0.05 with respect to the medium.
%, And cultured for 3 days. After replacing the same amount of medium and sample, the culture was further cultured for 3 days. The cells were detached by trypsin-EDTA treatment, and the obtained cells were centrifuged (1000 ppm, 1 minute) to remove the supernatant, and the hue of the cells was observed. A sample to which no test solution was added was used as a control.

[Table 1]

(Test Results) The results are shown in Table 1. As is clear from Table 1, an excellent whitening effect was observed in the Longan seed extract.

Example 4 <Antioxidant test> The freeze-dried powder of the longan seed extract prepared in Production Example 2 was subjected to 100 pp using a 50% aqueous ethanol solution.
m sample solutions were prepared. Reaction indicator (800 μM
1,1-diphenyl-2-picrylhydr
azyl 12 mL, 400 mM 2-morpholi
noethanesulphonic acid Bu
ffer 12 mL, ethanol aqueous solution 24 mL).
Dispense 9 mL into a test tube, and add (300-a) μL of a 50% aqueous ethanol solution. AL of the sample was added every 30 seconds, mixed with a vortex mixer, and the absorbance at 520 nm was measured after 20 minutes. a = 0, 30,
The volume was 60, 120, 180, and 240 μL. As a control, a freeze-dried powder of tea extract prepared in the same manner as in Production Example 2 was used.

(Test Results) The results are shown in FIG. As is clear from FIG. 3, a high antioxidant effect was observed in the Longan seed extract.

Example 5 <Production of lotion> According to the formulation shown in Table 2, (1) to (1)
0) was stirred and dissolved to produce a lotion.

[Table 2]

The obtained lotions were all at 40 ° C., RH7
It was stable for 3 months under the condition of 5%. In addition, a sensory test was conducted by 10 expert panelists. For the evaluation, the following items were evaluated on a 5-point scale.

(1) Skin moistness 1. Be bulky. 2. Slightly soft. Normal 4. 4. Slightly moist. Moist

(2) Skin smoothness 1 Rough 2 Slightly rough 3 Normal 4 Slightly smooth 5 Smooth

(3) Stickiness of skin 1 Sticky 2 Slightly sticky 3 Normal 4 Slightly refreshing 5 Refreshing The average of the scores of 10 panelists is shown in Table 3. As is clear from Table 3, the product of Example 4 in which the longan seed extract was blended exhibited better moisture retention and skin smoothness than the product of Comparative Example 1 in which the longan seed extract was not blended.

[Table 3]

Example 6 <Preparation of shampoo>
(4), (6) to (8) were mixed and stirred at 70 ° C.
After cooling, (5) was added and mixed and stirred to obtain a shampoo. When the hair was washed using the obtained product, the feel of the hair was smooth and the hair was moisturized. The obtained product was not sticky during use and moisturized the skin.

[Table 4]

Example 7 <Preparation of cream> (1)-
(7) was mixed and stirred at 80 ° C., and separately (8),
A mixture obtained by mixing and stirring (10) and (11) at 80 ° C. is added, homogenized, and cooled to 30 ° C. while stirring.
(9) was further added and mixed and stirred to produce a cream.

[Table 5]

Example 8 <Production of body gel> According to the formulation shown in Table 6, (1)
(8) was mixed and stirred at 80 ° C. to obtain a body gel agent. The obtained product was not sticky during use and moisturized the skin.

[Table 6]

Example 9 <Production of hair pack>
(2) was mixed and stirred at 80 ° C, and (3) to
The mixture obtained by mixing and stirring (13) at 80 ° C was added, and while mixing and stirring at 80 ° C, (14) and (15) were further added and mixed and stirred to obtain a hair pack agent. When hair treatment was performed using the obtained product, the feel of the hair was smooth and the hair was moisturized.

[Table 7]

[0067]

EFFECTS OF THE INVENTION The extract obtained from the longan seeds of the present invention makes it possible to effectively utilize the long discarded seeds which have been conventionally discarded, and has an anti-inflammatory effect, a collagenase activity inhibitory effect, a hyaluronidase. It has an activity inhibiting effect, a whitening effect, a moisturizing effect, and an antioxidant effect.
Therefore, a great effect is brought about by including the longan seed extract of the present invention in a skin external preparation. In other fields of use, it can be used as an external preparation for pharmaceuticals, quasi-drugs, cosmetics, and the like.

[Brief description of the drawings]

FIG. 1 is a diagram showing collagenase inhibitory activity.

FIG. 2 is a view showing a hyaluronidase inhibitory activity.

FIG. 3 shows antioxidant activity.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (reference) A61P 29/00 A61P 29/00 39/06 39/06 43/00 111 43/00 111 F term (reference) 4C083 AA111 AA112 AB442 AC022 AC072 AC102 AC122 AC182 AC352 AC392 AC422 AC432 AC482 AC642 AC712 AC792 AD042 AD092 AD202 AD322 AD352 AD392 AD442 AD512 CC02 CC04 CC05 CC33 CC38 DD23 DD31 DD41 EE01 EE06 EE07 EE10 EE12 AC28 ZB11 ZC20 ZC21

Claims (7)

[Claims] 1. An external preparation for skin, comprising a longan seed extract. 2. The external preparation for skin according to claim 1, wherein an extract of Longan seed is blended as a collagenase activity inhibitor. 3. The external preparation for skin according to claim 1, wherein an extract of longan seed is blended as a hyaluronidase activity inhibitor. 4. The external preparation for skin according to claim 1, wherein an extract of longan seed is blended as an anti-inflammatory agent. 5. The external preparation for skin according to claim 1, wherein the extract of Longan seed is blended as a whitening agent. 6. The external preparation for skin according to claim 1, wherein an extract of Longan seed is blended as a humectant. 7. The external preparation for skin according to claim 1, wherein an extract of Longan seed is blended as an antioxidant.