JP2002060317A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin care preparation which has a synergistically enhanced inhibitory action on melanin formation, is effective for preventing and improving pigmentation after sunburn, freckle, brown spot, liver spot, or the like, and has extremely improved bleaching effect on the skin and high safety. SOLUTION: This skin care preparation is formulated with one or more kinds selected from L-ascorbic acid and its salt or its derivative except an ascorbic acid glycoside, a 2-hydroxycarboxylic acid and its salt or its derivative, hydroquinone and its derivative, cysteine and its derivative, glucosamine and its derivative, azelaic acid and its derivative, an extract of placenta and an extract from a plant/alga having an inhibitory action on melanin production, and glycogen.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention has a synergistically enhanced melanin production inhibitory effect, is effective in preventing and improving pigmentation, spots, freckles, spots, etc. after sunburn, and has a markedly improved skin whitening effect. And a highly safe external preparation for skin.

[0002]

2. Description of the Related Art Conventionally, darkening of skin by ultraviolet rays,
In order to prevent or improve pigmentation of the skin, such as spots and freckles, components having an action of inhibiting melanin production or reducing the produced melanin pigment have been screened and formulated into skin external preparations. For example, ascorbic acid, cysteine, hydroquinone, placenta extract, 2-
Hydroxy acids and their derivatives, extracts from plants and algae and the like are used.

[0003] However, ascorbic acid, cysteine and hydroquinone are susceptible to oxidation-reduction reaction and are unstable, and 2-hydroxy acid has a problem in skin safety when an effective amount is added, placental extracts, plants and algae However, when an effective amount is added to the extract, there is a problem that an unpleasant odor or color may be imparted to the external preparation for skin.

[0004] Glycogen is a homopolysaccharide composed of D-glucose in the same manner as starch and amylopectin. From glycosyl chains of α-1,4 bonds of glucose, glycogen is averaged at a rate of about one every three units of glucose. Degree of polymerization 12
１８18 branches are formed by α-1,6 bonds, which also form a branched network structure. For this reason, it has been conventionally proposed that a skin external preparation is formulated as a moisturizer utilizing its structure (JP-A-62-1785).
05, JP-A-63-290809). Further, a fibroblast proliferating agent characterized by containing plant-derived phytoglycogen as an active ingredient has been disclosed (Japanese Patent Application Laid-Open No. Hei 11 (1999)).
-255657). However, it has not been known yet that glycogen is used in combination with a component having an effect of inhibiting specific melanin production to exert an effect of enhancing the effect.

[0005]

DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned problems, has a very strong melanin production inhibitory effect on the skin, is stable, has skin irritation and skin sensitization properties. The aim was to obtain an external preparation for the skin that had no safety problems.

[0006]

In order to solve the above-mentioned problems, various studies have been made. As a result, the whitening effect is synergistically improved by using a component having an action of inhibiting specific melanin production and glycogen in combination. The present inventors have found that an external preparation for skin which is enhanced and has no safety problems such as skin irritation and skin sensitization can be obtained, and the present invention has been completed.

[0007]

Embodiments of the present invention will be described.

[0008] Examples of L-ascorbic acid and salts or derivatives thereof other than ascorbic acid glycoside used in the present invention include L-ascorbic acid, sodium L-ascorbate, magnesium L-ascorbate and L-ascorbic acid. Potassium, L-ascorbate such as calcium L-ascorbate, L-ascorbate monostearate, L-ascorbate monopalmitate, L
L-ascorbic acid dialkyl or dialkenyl ester derivatives such as L-ascorbic acid distearate, L-ascorbic acid dipalmitate, L-ascorbic acid dioleate, etc., L-ascorbic acid L-ascorbic acid trialkyl or trialkenyl ester derivatives such as tristearate, L-ascorbic acid tripalmitate and L-ascorbic acid trioleate; L-ascorbyl sulfate;
L-ascorbic acid sulfates such as sodium ascorbyl sulfate, potassium L-ascorbyl sulfate, magnesium L-ascorbyl sulfate, calcium L-ascorbyl sulfate and salts thereof, L-ascorbyl phosphate, sodium L-ascorbyl phosphate, L-ascorbyl Potassium phosphate, L-ascorbyl magnesium phosphate, L-
L-ascorbic acid phosphates such as calcium ascorbyl phosphate and salts thereof can be mentioned. Of these L-ascorbic acid and salts or derivatives thereof, particularly preferred are L-ascorbic acid, L-ascorbyl phosphate and magnesium salts thereof.

The hinokitiol and derivatives thereof used in the present invention are not particularly limited, and include hinokitiol glycosides such as hinokitiol, hinokitiol zinc complex, hinokitiol glucoside, and acetyl derivatives thereof.

The 2-hydroxycarboxylic acid and salts or derivatives thereof used in the present invention are not particularly limited, but 2-hydroxycarboxylic acids having 2 to 22 carbon atoms are preferable, and 2-hydroxycarboxylic acids having 2 to 6 carbon atoms are more preferable. -Hydroxycarboxylic acid is preferably used in view of the effects of the present invention. For example, 2-hydroxyacetic acid, lactic acid, malic acid, tartaric acid, citric acid and salts thereof, and alkyl esters of 2-hydroxycarboxylic acid, cholesterol esters, glycosides, which improve the skin affinity of 2-hydroxycarboxylic acid, Phosphatidyl esters, L-arginine salts and the like.

The hydroquinone and its derivatives used in the present invention are not particularly limited, but hydroquinone glycosides are preferably used.
α-D-glucoside, hydroquinone-β-D-glucoside, hydroquinone-α-L-glucoside, hydroquinone-β-L-glucoside, hydroquinone-α-D
-Hexasaccharide glycosides such as -galactoside, hydroquinone-β-D-galactoside, hydroquinone-α-L-galactoside, hydroquinone-β-L-galactoside, hydroquinone-α-D-ribose, hydroquinone-β-
D-ribose, hydroquinone-α-L-ribose, hydroquinone-β-L-ribose, hydroquinone-α
Pentacarbon sugar glycosides such as -D-arabinose, hydroquinone-β-D-arabinose, hydroquinone-α-L-arabinose, hydroquinone-β-L-arabinose;
Hydroquinone-α-D-glucosamine, hydroquinone-β-D-glucosamine, hydroquinone-α-L-
Glucosamine, hydroquinone-β-L-glucosamine, hydroquinone-α-D-galactosamine, hydroquinone-β-D-galactosamine, hydroquinone-
amino sugar glycosides such as α-L-galactosamine and hydroquinone-β-L-galactosamine; hydroquinone-α
-D-glucuronic acid, hydroquinone-β-D-glucuronic acid, hydroquinone-α-L-glucuronic acid, hydroquinone-β-L-glucuronic acid, hydroquinone-
Uronic acid glycosides such as α-D-galacturonic acid, hydroquinone-β-D-galacturonic acid, hydroquinone-α-L-galacturonic acid, and hydroquinone-β-L-galacturonic acid can be exemplified. Examples of the derivative include an ester such as an acetylated product and an ether such as a methylated product. Among them, hydroquinone-β-D-glucose is most preferable from the viewpoint of the effect of the present invention.

The cysteine and derivatives thereof used in the present invention are not particularly limited.
Or phospholipid esters of cysteine, sphingosine and its derivatives, glycolipid esters, sugar esters, sterol esters, and alkyl or alkenyl esters having 8 to 20 carbon atoms.

The glucosamine and its derivatives used in the present invention are not particularly limited, and include, for example, glucosamine, glucosamine esters such as acetylglucosamine, and glucosamine ethers such as glucosamine methyl ether.

The azelaic acid and derivatives thereof used in the present invention are not particularly limited, but include, for example, azelaic acid, azelaic acid monoesters such as azelaic acid monoalkyl ester, and azelaic acid diesters such as azelaic acid dialkyl ester. Is mentioned.

The placenta extract used in the present invention may be of any origin as long as it is used in a usual external preparation for skin.

[0016] Plants to obtain extracts for use in the present invention, the algae, especially the type is not limited as far as extracts from plants and algae melanin production inhibitory activity is known, witch hazel (Hamamelis japonica Sieb. Et Zucc.), Cinnamon Saku ( Hamamelis mollis Oliv.), Hamameris ( Hamame
lis virginiana L.) witch hazel plant of the genus, etc., Hoshitsudzuri (Saxifraga aizoon Jacq.), Shikotansou (Saxifrag
a cherlerioides D. Donvar. rebunshirensis (Engl . e
t Irmsch) Hara, Saxifraga bronchialalis L.), ginjisou ( Saxifraga cortusaefolia Sieb. et Zucc.),
Squirrel ( Saxifraga fortunei Hook. F. Var. I
ncisolobata (Engl. et Irmsch.) Nakai), Saxifraga nipponica Makino, Sendaisou ( Saxifraga sendaica Maxim.), Saxifra
ga stolonifera Meerb.), Saxifraga ( Saxifraga )
j aponica Boiss.), black moss ( Saxifraga fusca )
Maxim.), Spider Magsa ( Saxifraga merkii Fish. Var.
laciniata Nakai), Sakumai Sakura ( Saxifraga lac)
iniata Nakai et Takeda), Shikotanso ( Saxifraga )
bronchialalis L.), Mukagoyukinosita ( Saxifraga ce)
rnua L.) and other saxifrage plants, Jinko ( Aquilaria)
agallocha Roxb.), Camellia japonica L., its variants and tea ( Came)
Camellia plants, such as llia sinensis (L.) O. Kuntze) and its variants, safflower ( Aesculus carnea Hayn)
e), Aesculus chinensis Bunge, Aesculus chinensis Bunge, Aesculus hippocastanum L., Aesculus genus plants such as Aesculus turbinata Bl.
Knotweed ( Polygonum cuspidatum Sieb. Et Zucc.),
Honeybird ( Polygonum cuspidatum Sieb. Et
Hachidyoense Ohwi), Giant Knotweed ( Polyg )
onum sachalinense Fr. Schm.) Polygonum plants such, oilseed isodon plants typified by Melissa (Melissa officinalis L.), Ibuki thyme (Thymus serph
yllum L. subsp. quinquecostatus (Aelak. ) Kitamur
a), thyme ( Thymus vulgaris L.) and the like, plants of the genus Artemisia absinthium
L.), Artemisia annua (Artemisia annua L.), Kawara carrots (Artemisia apiacea Hance), Artemisia capillaris (A
rtemisia capillaris Thunb.), Artemisi
a cina Berg.), Tarragon ( Artemisia dracunculus )
L.), Artemisia japonica Thunb.,
Mibuyomogi (Artemisia maritima L.), wormwood (Artem
Artemisia plants such as isia princeps Pamp.), Yarrow ( Achillea alpina L.), Yarrow ( Ac )
hillea milleifolium L.), gypsophila ( Ac )
hillea moschata Jacq., etc., E. spp. ( Eupatorium japonicum Thunb.), Sawa's bulbul ( Eupatorium lindleyanum DC.), Bulbul ( Eupatorium chinense L. var. oppositifolium (Koid
z.) Murata et H. Koyama)
American linden ( Tilia americana L.), scallop ( Tiliacordata Mill.), Linden ( Tili )
a europaea L.), linden ( Tilia japonica (Miq.) Si
Monk.), Tilia miqueliana Maxim., Tilia platyphyllos Scop., etc., linden plants, Hypericum ascyron L., Hypericum erectum Thunb., Hypericum japonicum Thunb. Hypericum plants such as St. John's wort ( Hypericum perforatum L.),
Iva saxifrage (Tanakaea radicans Fr. et Sav.) On a rock Saxifraga plant to be representative, Satsumafuji (Daphne
genkwa Sieb. etZucc.), camphor tree ( Daphne kius )
iana Miq.), Daphne mezereum
L.), Daphne odora Thunb., Onishibari ( Daphne pseudo-mezereum A. Gray), Naniwazu ( Daphne kamtchatica Maxim. Var. Yezoensis Ohwi),
Daphne miyabeana Makino and other Daphne genus plants, Diplomorpha phymatog
lossa (Koidz.) Nakai), Ganpi ( Diplomorpha sikoki )
ana (Fr. et Sav.) Honda), Kiganpi ( Diplomorpha )
trichotoma (Thunb.) Nakai), Ganpi plants such as mithota ( Edgeworthia chrysantha Lindl.), Paeonia lactiflora Pal
l.), Dutch Peony ( Paeonia officinalis)
L.), button ( Paeonia suffruticosa Andr.), And other button genus plants, Glycyrrhiza glabra
L.), Kikanzou (Glycyrrhiza kansuensis Chang et
peng), licorice ( Glycyrrhiza urarensis Fisch.), liquorice plants represented by mulberry ( Morus alba L.), Clara ( Sophora flavescens Ait.),
Plants of the genus Genus such as Enju ( Sophora japonica L.), plants of the genus Genus such as char ( Epigaea asiatica Maxim.), American char ( Epigaea repens L.),
Nagase-no-moss ( Drosera angelica Huds.), Ichomochisou ( Drosera peltata Smith), Datura moss ( Drosera routundifolia L.), Komasen-moss ( Dros
era spathulata Labill.) and other species of the genus Araceae, Urashi azalea ( Arctostaphylos alpina (L.) Spreng. v
ar. japonica (Nakai) Hulten) , bearberry (Arctost
aphylos uva-ursi (L.) Spreng.) and other species of the genus Rhododendron, sp. alga represented by Ceratodictyon spongiosum , and coralless coral ( Corall.
ina sp.), coralline (Corallina officinalis), coralline algae such Pirihiba (Corallinapilurifera), Yahazugusa (Dictyopteris latiuscula), Shiwayahazu (D
ictyopteris undulata ), Herayahaz ( Dictyopteris p.
rolifera), Sujiyahazu (Dictyopteris plagiogramm
a ), Himeyahazu ( Dictyopteris repens ), Ezoyahazu ( Dictyopteris divaricata ), Uraboyahazu ( Dicty )
opteris polypodioides) Yahazugusa algae such as, Hariamiji (Amijigusa algae typified Dictyota spinulosa), hijiki algae typified hijiki (Hizikia fusiformis), Sozo sp. (Laurencia sp.), Kurosozo (La
urencia intermedia ), mitsudezo ( Laurencia okamur )
ai ), Sozonohana ( Laurencia grevilleana ), Osozo ( Laurencia glandulifera ), Hanesozo ( Laurencia )
pinnata), Kobusozo (Laurencia undulata) Sozo algae such as, Fushitsunagi (Lomentaria catenata), Kosujifushitsunagi (Lomentaria hakodatensis) Fushitsunagi algae such as, cassiope lycopodioides algae typified cassiope lycopodioides (Myelophycus caespitosus), dulse (Palmaria palmata)
Algae typified by the genus Darussa, Sargassum fu
lvellum ), pea ( Sargassum yendoi ), beetle ( Sargassum piluriferum ), and yam ( Sarg
assum patens ), red moku ( Sargassum horneri ), saw-toothed moku ( Sargassum serratifolium ), giant-faced sawtoothed moku ( Sargassum giganteifolium ), and yolemoku ( Sa
rgassum tortile ), willow moku (Obamoku: Sargass)
um ringgoldianum ), Nejimoku ( Sargassum sagamianu )
m ), Hahakimoku ( Sargassum kjellmanianum ), Sea turtle ( Sargassum thunbergii ), Fushijimoku ( Sarg
assum confusum ), isomok ( Sargassum hemiphyllu )
m ), Narasamo ( Sargassum nigrifolium ), Togemoku ( Sargassum micracanthum ), Tamanashimoku ( Sargassu
m nipponicum ), gypsophila ( Sargassum vulgare ),
Futamomoku (Holly moku: Sargassum duplicatum ),
Examples include algae belonging to the genus Hondara such as Sargassum yezoense, and algae belonging to the genus Isomozuku represented by Sphaerotrichia divaricata .

Among the above-mentioned plants and algae, Hamameli
Su (Hamamelis virginiana L.)Saxifra
ga stolonifera Meerb.), Jinko (Aquilaria agall
ocha Roxb.), Cha (Camellia sinensis (L.) O. Kunt
ze) and its variant, knotweed (Polygonum cuspidatum S
ieb. et Zucc.), Melissa (Melissa officinalis
L.), time (Thymus vulgaris L.), mugwort
(Artemisia capillarisThunb.), European saw
CAchillea milleifolium L.), Hypericum (Hype
ricum erectum Thunb.), Hypericum perforatum (Hypericu
m perforatum L.), peonies (Paeonia lactiflora 
Pall.), Button (Paeonia suffruticosaAndr.) 、 Spec
Incan elephant (Glycyrrhiza glabra L.), Licorice (G
lycyrrhiza urarensis Fisch.), Mulberry (Morus alba 
L.), Clara (Sophora flavescensAit.) 、 Iwanashi
(Epigaea asiatica Maxim.), American char (Epi
gaearepens L.)Drosera routundifol
ia L.), Uwaurushi (Arctostaphylos uva-ursi (L.)
Spreng.), Herayahaz (Dictyopteris prolifera),
Haramiji (Dictyota spinulosa), Hijiki (Hizikia 
fusiformis), Sozo sp. (Laurencia sp.)
(Lomentaria catenata), Mustache (Myelophycus cae
spitosus), Darth (Palmaria palmata), Willow moku
(Oobamoku:Sargassum ringgoldianum), Ezonone
Jimok (Sargassum yezoense), Fushijimoku (Sargas
sum confusum) 、 Ishimozuku (Sphaerotrichia divaric
ata) Is selected from one or more plants and algae
It is preferably used.

The extracts from these plants and algae are used as they are or after drying one or more places such as leaves, bark, roots, flowers, branches and the like of various kinds of whole plants. The extraction solvent is not particularly restricted but includes water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol,
Monohydric alcohols such as n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol, etc. Polyhydric alcohols or derivatives thereof, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, and methyl-n-propyl ketone; esters such as ethyl acetate and isopropyl acetate; ethers such as ethyl ether, isopropyl ether and n-butyl ether , Squalane, petrolatum, paraffin wax, paraffin oil and other hydrocarbons, olive oil, wheat germ oil, rice oil, sesame oil, macadamian nut oil, almond oil, yam Vegetable oils such as oil, beef tallow, lard, animal oils and fats, such as whale oil and the like. Further, a polar solvent to which an inorganic salt such as a phosphate buffered saline is added, and a solvent to which a surfactant is added can be used, and there is no particular limitation.

Further, as the extraction method, a method of extracting by impregnation at room temperature, in a cooled or heated state, a method of extraction using a distillation method such as steam distillation, a method of pressing a plant or algae to obtain an extract For example, these methods are used alone or in combination of two or more.

The ratio of the plant or algae to the solvent at the time of extraction is not particularly limited.
0.1 to 1000 times by weight, especially in terms of extraction operation and efficiency.
5 to 100 times by weight is preferred. It is convenient to set the extraction pressure and the extraction temperature in a range from 0 ° C. to the boiling point of the solvent at normal pressure, and the extraction time varies depending on the extraction temperature.
It is preferable to set the time in a range from time to two weeks.

The extract of the plant or algae thus obtained can be used as it is, but it may be subjected to purification operations such as deodorization, decolorization and concentration, as long as the effect is not lost. May be used as a fraction using column chromatography or the like. These extracts, purified products, and fractionated products can be dried by removing the solvent therefrom, and can be used in a form solubilized in a solvent such as alcohol or in an emulsion form. it can.

The components obtained by purification and fractionation from the plant extract include hamamelitannins, which are often contained in hamamelis leaves, and flavonoids, particularly glabridine and hispagrabridine, which are contained in oil-soluble extract components of licorice or licorice. And (-)-epicatechin contained in an extract of the genus Polygonum.

The amount of the component having an effect of inhibiting melanin production in the external preparation for skin is considered to be 0.0001 to 10% by weight in consideration of its effect and odor and color tone when added. It is desirable to do.

The source of glycogen used in the present invention may be of animal or plant origin. Examples of animals include shellfish such as scallops, abalones, oysters, mussels, and livers of cattle and pigs. Examples of plants include seeds of corn, barley, rice and the like.

The method of extracting glycogen used in the present invention is not particularly limited, and any method can be adopted. A general preparation method is as follows. After making a biological tissue containing glycogen easy to extract by pulverization or the like, the solid content of the biological tissue is extracted by adding hot water 3 to 20 times by weight with respect to the solid content of the biological tissue. Other insolubles are removed by centrifugation or the like. The resulting extract is treated as it is or after treatment with trichloroacetic acid or the like to remove proteins, and then an organic solvent such as methanol, ethanol, acetone or the like is added to the treated solution to precipitate glycogen, or a method such as spray drying or freeze drying. To obtain glycogen in powder form.

The amount of glycogen incorporated into the external preparation for skin is not particularly limited, but is generally 0.0001 to 10% by weight, preferably 0.001 to 5% by weight.

The external preparation for skin of the present invention may contain, if necessary,
Oils and fats, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, ultraviolet absorbers, thickeners usually used in pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics, and cleansers , Drugs, fragrances, resins, alcohols and the like can be appropriately compounded. Further, the dosage form of the external preparation for skin of the present invention is arbitrary, and for example, it can be provided as a solubilizing system such as a lotion, an emulsifying system such as a cream or an emulsion, or a dispersion system such as a calamine lotion. An aerosol dosage form filled together with the aerosol may be used.

[0028]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples. First, preparation examples of glycogen, placenta extract, and plant and algal extracts used in the present invention will be described.

[Glycogen Derived from Mussels] 50 ml of purified water at 75 ° C. was added to 100 g of mussel shell meat, and the mixture was allowed to stand for 15 minutes. Centrifuge at 800rpm,
About 5% by weight of trichloroacetic acid was added to the obtained separated liquid to remove the protein, followed by filtration. The filtrate was concentrated by a membrane filter, and then spray-dried with a spray dryer to obtain mussel-derived glycogen.

[Glycogen derived from sweet corn] 100 g of fresh ground corn seeds and 100 g of hot water
After adding ml and mixing, the mixture is filtered through a 200-mesh sieve. The obtained filtrate was centrifuged at 8,500 rpm to remove proteins and other insolubles. After filtering the supernatant with filter paper, 95
After heating at 20 ° C. for 20 minutes and cooling, the mixture was centrifuged at 5000 rpm to remove the coagulated protein. The supernatant was cooled to 4 ° C., and trichloroacetic acid was added thereto to a concentration of 5% by weight.
After standing at ℃ overnight, the precipitate was removed by centrifugation at 5000 rpm, the supernatant was poured into 3 times the volume of methanol, and the resulting precipitate was collected by centrifugation at 5000 rpm, and ethanol, methanol, After successive washing with diethyl ether,
Vacuum drying was performed to obtain sweet corn-derived glycogen.

[Placenta extract] A commercially available water-soluble placenta extract (manufactured by Nichirei) was used.

[Kitadori extract] Knotweed ( Polygonum
cuspidatum Sieb. et Zucc.)
It was pulverized and stirred and extracted in 1,500 ml of a 50% by volume aqueous ethanol solution at 25 ° C. for 5 days. Next, the extract was filtered, and the filtrate was collected to obtain a knotweed extract.

[ Kitadori Extract Fraction] Knotweed ( Polygo
dry powder 2 of rhizome of num cuspidatum Sieb. et Zucc.)
00 g was immersed in 2,000 ml of a 50% by volume aqueous ethanol solution and extracted at room temperature for 7 days. Next, the extract was filtered, the filtrate was concentrated under reduced pressure, dissolved in 800 ml of a 30% by volume aqueous ethanol solution, and the DIAION MCI Gel was dissolved.
Apply to an HP-20 column (manufactured by Mitsubishi Chemical Corporation) and apply
The fraction eluted with a 0% by volume aqueous ethanol solution was collected. Subsequently, the fraction was subjected to silica gel thin layer chromatography to obtain a chloroform / methanol mixture (volume ratio = 5:
Fractionation was performed using 1) as a developing solvent. Among the obtained fractions, the fraction containing (-)-epicatechin was scraped off and dissolved in 50 ml of 50% by volume ethanol to obtain a knotweed extract fraction.

[ Hamelis extract] Hamamelis ( Hameme
lis virginiana L.) 500 g of leaves are dried, crushed and
25 ° C in 1,000 ml of 0 volume% ethanol aqueous solution
For 5 days. Next, the extract was filtered, and the filtrate was collected to obtain a Hamamelis extract.

[Hamellis extract fraction] Hamamelis ( Ha
(mamelis virginiana L.), and a total of 500 g of leaves and bark were minced and extracted with stirring in 1,500 ml of hot water for 5 hours.
Then, the extract is filtered, and the filtrate is filtered by DIAION MCI.
It was applied to a Gel HP-20 column (manufactured by Mitsubishi Kasei Corporation) and eluted with a mixed solvent of ethanol and water. A fraction having a hamamelitannin content of 50% by weight or more was collected and used as a hamamelis extract fraction.

[ Hypericum perforatum extract] Whole plant 35 of flowering season of Hypericum perforatum L.
0g is finely chopped and 1,000ml of 1,3-butylene glycol
It was immersed at 20 ° C. for 7 days to extract. The extract was filtered and the filtrate was collected to obtain a Hypericum perforatum extract.

[Licorice extract] Spanish licorice ( Glycyr)
rhiza glabra L.) roots and rhizomes (500 g) are dried and pulverized, and are dried at 25 ° C. in 1,000 ml of anhydrous ethanol aqueous solution.
For 24 hours. The extract is filtered to collect the filtrate, concentrated under reduced pressure, and lyophilized to dryness. The dried product was stirred and extracted in 1,000 ml of ethyl acetate at 20 ° C. for 2 days. After extraction, it was dried under reduced pressure to obtain a licorice extract.

[ Goldfish extract] Ginkgo ( Aquillaria)
agallocha Roxb.) was dried and pulverized, and immersed in ethanol 1,000 at 20 ° C. for 10 days. Next, the extract was filtered, and the filtrate was concentrated to 1/10 volume to obtain a ginger extract.

[Oolong tea extract] Oolong tea ( Thea s
inensis L. var. viridis Szkzyl.) leaves were dried and pulverized, and dried at 50 ° C. in 1,000 ml of purified water for 24 hours.
The mixture was stirred and extracted for hours. The extract was filtered, and the filtrate was concentrated to 1/5 volume to obtain an oolong tea extract.

[Melissa extract] Melissa of
ficinalis L.) leaves were crushed and extracted with stirring in 1,000 ml of 1,3-butylene glycol at 25 ° C. for 5 days. The extract was filtered, and the filtrate was collected to obtain a Melissa extract.

[Thyme extract] Thyme ( Thymus vulgari
s L.) was dried and pulverized, and extracted with stirring in 1,500 ml of a 50% by volume aqueous glycerin solution at 25 ° C. for 5 days. The extract was filtered, and the filtrate was collected to obtain a thyme extract.

[Button extract] button ( Paeonia suffru)
ticosa Andr.) is dried and pulverized, and is dried at 25 ° C. in 1,000 ml of a 95% by volume aqueous ethanol solution at 25 ° C.
Extracted with stirring for days. The extract was filtered, and the filtrate was collected to obtain a button extract.

[Mulberry Extract] 350 g of mulberry ( Morus alba L.) root bark is minced, and 1,3-butylene glycol 1,0
It was immersed in 00 ml at 20 ° C. for 7 days for extraction. The extract was filtered and the filtrate was collected to obtain a mulberry extract.

[ Achillea millefolium extract] Flowers 320 of Achillea milleifolium L.
g was homogenized in 2,000 ml of physiological saline at 10 ° C., and further extracted with stirring for 4 hours. The extract was filtered, and the filtrate was collected to obtain an Achillea millefolium extract.

[American char pear extract] 450 g of leaves of American char pear ( Epigaea repens L.) were dried, pulverized, and extracted with stirring in 1,500 ml of absolute ethanol at 25 ° C for 5 days. The extract was filtered, and the filtrate was collected to obtain a American chard extract.

[0046] [sundew extract] sundew (Dr
osera routundifolia L.) was crushed and extracted with stirring in 1,000 ml of absolute ethanol at 25 ° C. for 5 days. The extract was filtered, and the filtrate was collected to obtain an A. moss extract.

[ Erythrophilus extract]
taphylos uva-ursi (L.) Spreng.) leaves (350 g) were minced and dissolved in 1,000 ml of 1,3-butylene glycol for 20 minutes .
Extraction was performed by immersion at 7 ° C. for 7 days. The extract was filtered, and the filtrate was collected to obtain a mulberry extract.

[0048] [Herayahazu extract], [Hariamiji extract], [Sozo sp. Extract], [dulse extract] was taken from the sea, Herayahazu (Dictyopteris prolifera), Hariamiji (Dict yota spinulosa), Sozo sp. ( Laurencia s
p.) and dulse ( Palmaria palmata ) were washed with water, cut into small pieces, dispersed in an equal volume of phosphate buffered saline (pH 7.4), and then extracted with stirring in a blender mill for 3 hours. The extract was filtered, and the filtrate was collected to obtain each of the above extracts.

According to the formulation shown in Table 1, Examples 1 to 12 and Comparative Examples 1 to 12 were prepared as cosmetics according to the present invention. These serums were prepared by mixing and homogenizing all components. In addition, all the items were shown by weight%.

[0050]

[Table 1]

[0051]

[Table 2]

[0052]

[Table 3]

Examples 1 to 3 of the present invention prepared according to the above formula
Example 12 and Comparative Examples 1 to 12 were evaluated for the effect of improving pigmentation symptoms. The effect of improving the pigmentation symptom was such that 20 female panelers having remarkable pigmentation symptoms such as spots and freckles were grouped into a group, and each group was blinded with Examples 1 to 12 and Comparative Examples 1 to 12 respectively. The skin was used twice a day for one month, and the state of pigmentation of the skin after one month was observed and compared with that before use. The state of pigmentation was evaluated according to the criteria shown in Table 4,
Tables 5 and 6 show the average values of the 20 subjects.

[0054]

[Table 4]

[0055]

[Table 5]

[0056]

[Table 6]

As is clear from Tables 5 and 6, in the group using the examples according to the present invention, remarkable improvement of the pigmentation symptom was observed in all the groups, and after the end of the use test, it was mild or slight. The symptoms had been improved to the extent that pigmentation was only observed. In particular, good improvement was observed in Example 1 in which glycogen was used in combination with L-ascorbyl magnesium phosphate, Example 8 in which a knotweed extract was used in combination, and Example 9 in which a hamamelis extract was used in combination. Had been. On the other hand, in a comparative example use group in which a substance having a melanin production inhibitory action was independently blended,
Although the pigmentation symptom could be improved, the degree of improvement was clearly smaller than in the corresponding groups using the examples.

In Examples 1 to 12 of the present invention, no change in the state of the preparation such as precipitation, separation, agglomeration, discoloration, and discoloration of the components was found at all during the use test period. In addition, in each group used in Examples, there was no paneler showing a skin irritating reaction or a skin sensitizing reaction.

Another embodiment of the present invention will be described.

Example 13 Serum (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 32.5 (5) Fatty Acid Glycerin 0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20EO) sorbitan monolaurate 2.0 (8) Propylene glycol 5.0 (9) Sweet corn-derived glycogen 0.4 (10 ) Sodium lactate 0.3 (11) Purified water 34.8 Production method: The oily components (1) to (7) and the aqueous components (8) to (11) are mixed and homogenized, respectively, and heated to 75 ° C. The oily component is added to the aqueous component, emulsified, and cooled with stirring.

Example 14 Essence (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Poly Oxyethylene oleyl ether (20EO) 1.2 (6) Propylene glycol 5.0 (7) Purified water 58.2 (8) Carboxyvinyl polymer (1% by weight aqueous solution) 20.0 (9) Mussel-derived glycogen 1.5 (10) Potassium hydroxide 0.1 (11) Ethanol 5.0 (12) Fragrance 0.2 (13) L-ascorbic acid 0.5 Production method: The oil phase components of (1) to (5) are mixed, Heat to 75 ° C to dissolve and homogenize. On the other hand, the aqueous phase components (6) to (9) were mixed and dissolved, heated to 75 ° C., added with the oil phase component, uniformly emulsified with a homomixer, and added with (10) to adjust the pH. To adjust. After cooling, add (11) to (13) at 40 ° C and mix.

[Example 15] Lotion for skin (1) Ethanol 10.0 (wt%) (2) Hydroxyethylcellulose 1.0 (3) Sweet corn-derived glycogen 1.0 (4) Glycerin 7.0 (5) Sodium guaiazulene sulfonate 0.5 (6) α-hydroxyacetic acid 0.5 (7) Purified water 80.0 Production method: Mix (1) to (7) to make uniform.

Example 16 Emulsion for Skin (1) Stearic acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Vaseline 3.0 (4) Liquid paraffin 7.0 (5) Polyoxy Ethylene (10EO) monooleate 1.5 (6) Glycerin 5.0 (7) Triethanolamine 1.0 (8) Mussel-derived glycogen 1.0 (9) Purified water 79.0 (10) Lactic acid bacteria extract 0.5 (11) Placental extract 0.3 Production method: Mix and heat the oil phase components of (1) to (5) to dissolve uniformly and keep at 70 ° C. On the other hand, the aqueous phase components (6) to (9) are mixed and heated to be uniform, and the temperature is set to 70 ° C. The oil phase component is gradually added to the aqueous phase component with stirring, emulsified, cooled, and the components (10) and (11) are added at 40 ° C.

Example 17 Skin Gel (1) Purified Water 90.9 (wt%) (2) Carboxyvinyl Polymer 0.5 (3) Sweet Corn-Derived Glycogen 0.3 (4) Dipropylene Glycol 8 0.0 (5) Potassium hydroxide 0.1 (6) Hinokitiol-β-D-glucoside 0.2 Production method: After (2) and (3) are uniformly dissolved in (1), (4) is added. Then, (5) is added to thicken, and (6) is added and mixed uniformly.

Example 18 Skin Cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 29.5 (5) Lipophilic Type Glyceryl monostearate 4.0 (6) Polyoxyethylene (20EO) sorbitan monolaurate 5.0 (7) Propylene glycol 5.0 (8) Mussel-derived glycogen 0.5 (9) Oolong tea extract 0.2 (10) Purified water 36.8 Production method: Mix and dissolve the oil phase components (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved and heated to 75 ° C. Next, after the oil phase component is added to the water phase component and pre-emulsified, the mixture is uniformly emulsified by a homomixer.

Example 19 Oil-in-water emulsion ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Sodium lauryl sulfate 1.0 (4) Glycerin 10.0 (5) Glycogen derived from sweet corn 0.5 (6) Melissa extract 0.2 (7) Purified water 38.3 Production method: The oil phase components of (1) to (3) are mixed and dissolved to make uniform, and 75 Heat to ° C. On the other hand, (4) to (6) are dissolved in (7) and dissolved by heating at 75 ° C., and the oil phase component is added to this and emulsified.

Example 20 Lotion (1) Ethanol 10.0 (% by weight) (2) 1,3-butylene glycol 10.0 (3) Hinokitiol-β-D-glucoside tetraacetate 0.5 (4 ) Dipotassium glycyrrhizinate 0.5 (5) Mussel-derived glycogen 0.2 (6) Fragrance 0.1 (7) Purified water 78.7 Production method: (1) to (6) are sequentially added to (7) to be uniform Mix and dissolve.

Example 21 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self Emulsified glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Sweet corn-derived glycogen 0.5 (8) Purified water 69.0 (9) Titanium oxide 1.0 (10) Bengala 0.1 (11) Yellow iron oxide 0.4 (12) Fragrance 0.1 (13) Button extract 0.1 Production method: Mix oil phase components (1) to (4) And heated to 75 ° C. to make it uniform. On the other hand, the components (5) to (8) were mixed and 75
The mixture was heated and dissolved at ℃ to make it uniform, and the pigments of (9) to (11) were added thereto and uniformly dispersed with a homomixer to obtain an aqueous phase component. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and the components (12) and (13) are added and mixed at 40 ° C.

Example 22 Emulsion Foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 8.0 (7) Potassium hydroxide 0.1 (8) Mussel-derived glycogen 0.3 (9) Purified water 51.2 (10 ) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Mulberry extraction Product 0.2 Production method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. to make uniform. On the other hand, the aqueous phase components (6) to (9) are mixed,
The mixture is heated to 75 ° C. and dissolved to make the mixture uniform, and the pigments (10) to (14) are added thereto and uniformly dispersed by a homomixer. After adding the oil phase component and emulsifying, the mixture is cooled and cooled at 40 ° C. (15),
Add and mix component (16).

Example 23 Hand Cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO) glyceryl isostearate 2.5 (6) Tocopherol acetate 0.5 (7) Glycerin 20.0 (8) Methyl parahydroxybenzoate 0.1 (9) Sweet corn-derived glycogen 0.3 (10 ) Hydroquinone-β-D-glucoside 0.1 (11) Purified water 59.0 Production method: Mix and dissolve the oil phase components (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (11) are mixed and dissolved and heated to 75 ° C. Then, after the oil phase component is added to the water phase component and pre-emulsified, the mixture is uniformly emulsified by a homomixer.

Example 24 Jelly-like peel-off pack (1) Polyvinyl alcohol 15.0 (% by weight) (2) Carboxymethyl cellulose 5.0 (3) 1,3-butylene glycol 3.0 (4) Ethanol 0 (5) Polyoxyethylene (20EO) oleyl ether 0.5 (6) Mussel-derived glycogen 0.2 (7) Achillea millefolium extract 0.1 (8) Purified water 70.2 Manufacturing method: (8) ) And heat to 75 ° C. to this
(1) and (2) are added for dissolution, and (4) to (7) are added for solubilization.

Example 25 Massage Gel (1) Dipropylene glycol 7.0 (% by weight) (2) Glycerin 8.0 (3) Polyoxyethylene (15EO) oleyl ether 1.0 (4) Carboxyvinyl polymer 0.4 (5) Methylcellulose 0.2 (6) Sweet corn-derived glycogen 0.4 (7) Thyme extract 0.1 (8) Ginger extract 0.1 (9) Potassium hydroxide 0.1 (10) Purified water 82.7 Production method: Components (1) to (9) are sequentially added to (10) heated to 75 ° C., dissolved and homogenized.

Example 26 Face Wash (1) Stearic acid 2.0 (% by weight) (2) Cetanol 3.0 (3) Vaseline 10.0 (4) Liquid paraffin 33.0 (5) Isopropyl myristate 7.5 (6) Glyceryl monostearate 2.5 (7) Polyoxyethylene (20EO) sorbitan monostearate 2.5 (8) Glycerin 5.0 (9) Sweet corn-derived glycogen 0.3 (10 ) Potassium hydroxide 0.1 (11) Purified water 33.9 (12) Herayahazu extract 0.2 Production method: The oil phase components of (1) to (7) are mixed and dissolved by heating.
° C. On the other hand, the aqueous phase components (8) to (11) are mixed and dissolved by heating to 70 ° C. The oil phase component is gradually added to the aqueous phase component for pre-emulsification, then uniformly emulsified by a homomixer, cooled, and the component (12) is added at 40 ° C.

Example 27 Skin Lotion (1) Ethanol 20.0 (% by weight) (2) Hydroxyethylcellulose 1.0 (3) Mussel-derived glycogen 0.2 (4) Glycerin 7.0 (5) Guaiazulene Sodium sulfonate 0.5 (6) Haramidi extract 0.2 (7) Purified water 71.1 Production method: (1) to (7) are mixed and made uniform.

Example 28 Gel for Skin (1) Purified water 84.2 (wt%) (2) Carboxyvinyl polymer 0.5 (3) Sweet corn-derived glycogen 0.1 (4) 1,3- Butylene glycol 15.0 (5) Sozo sp. Extract 0.1 (6) Potassium hydroxide 0.1 Manufacturing method: (2) to (5) are added sequentially to (1) and uniformly dissolved,
Add (6) to thicken.

Example 29 Lotion (1) Ethanol 10.0 (% by weight) (2) 1,3-butylene glycol 10.0 (3) Mussel-derived glycogen 0.1 (4) Dipotassium glycyrrhizinate 5 (5) Dulce extract 0.2 (6) Fragrance 0.1 (7) Purified water 79.1 Production method: The components (1) to (6) are sequentially added to (7), mixed,
Make uniform.

In Examples 13 to 29 of the present invention, the effects of improving skin irritation and pigmentation symptoms were examined.
For skin irritation, a 48-hour obstruction sticking test was performed by 20 male panelists, and the results were determined according to the criterion shown in Table 7, and indicated by the average value of the skin irritation index of 20 persons. The jelly-like peel-off pack of Example 24 was peeled off 20 minutes after application and then closed, and the face wash of Example 26 was evaluated with a 1% by weight aqueous solution. The improvement effect of the pigmentation symptoms was obtained by grouping 10 female panelers who had remarkable pigmentation symptoms such as spots and freckles, using each of the examples twice a day blindly for one month. The state of pigmentation of the skin after months was observed, and the improvement effect was shown by the number of panelists who were evaluated to have an improvement effect compared to before use. Table 8 shows the results of the effects of improving skin irritation and pigmentation symptoms.

[0078]

[Table 7]

[0079]

[Table 8]

As shown in Table 8, the examples of the present invention showed no skin irritation, good safety, and an excellent effect of improving pigmentation.

[0081]

Industrial Applicability As described in detail above, the present invention synergistically enhances the melanin production inhibitory effect, is effective in preventing and improving pigmentation, spots, freckles, spots, etc. after tanning. A highly safe skin external preparation with significantly improved whitening effect was obtained.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 7/50 A61K 7/50 31/05 31/05 31/122 31/122 31/19 31/19 31/194 31 / 194 31/198 31/198 31/375 31/375 31/7008 31/7008 31/716 31/716 35/50 35/50 35/78 35/78 CT 35/80 35/80 Z A61P 17 / 16 A61P 17/16 F-term (reference) 4C083 AA032 AA071 AA072 AA082 AA111 AB032 AB232 AB242 AB432 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC301 AC302 AC352 AC402 AC422 AC442 AC482 AC542 AC782 AC792 AD092 AD112 AD2AD1 AD642 AD662 CC04 CC05 CC07 CC12 CC23 EE16 4C086 AA01 BA18 EA02 EA20 MA03 MA28 NA14 ZA89 4C087 AA01 BB58 MA02 NA14 ZA89 4C088 AB12 AB26 AB29 AB34 AB38 AB44 AB45 AB58 AB60 AB66 BA08 MA05 MA07 MA28 NA14 ZA89 MA28 NA14 ZA89 MAC

Claims (2)

[Claims] 1. L-ascorbic acid and its salts or derivatives thereof, hinokitiol and its derivatives, 2-hydroxycarboxylic acid and its salts or its derivatives, hydroquinone and its derivatives, cysteine and its derivatives, excluding ascorbic acid glycosides, An external preparation for skin containing glycogen and one or more selected from glucosamine and its derivatives, azelaic acid and its derivatives, placental extracts, and plant / alga extracts having melanin production inhibitory activity, and glycogen. 2. A plant / algae extract having a melanin production inhibitory action, wherein said extract is of the genus Mansacea, Saxifraga, Zinko, Camellia, Aesculus, Polygonum, Acacia genus,
Genus Genus, Artemisia, Yarrow, Yarrow, Genus Periwinkle, Rhododendron, Hypericum, Genus Ilex, Genus Rosa, Genus Gampi, Mitsumata, Button Genus, Genus Genus, Mulberry, Genju, Genus Genus, Genus Selected from the group consisting of the plants of the genus Urashi and the extracts from algae of the genus Spiraeus, Coralum, Phytopathus, Aphididaceae, Hijiki, Sozo, Fushitsunagi, Ichihige, Darus, Hondawara and Ishimozuku One or two or more
The external preparation for skin according to claim 1.