JP2003081744A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an antioxidant containing, as an effective component, a substance that has been discovered in naturally-occurring substances and has an active oxygen and/or its radical scavenging activity. SOLUTION: This antioxidant contains an extract from an aboveground part of licorice as the active ingredient.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antioxidant containing a plant extract as an active ingredient, particularly an antioxidant containing a plant extract having an antioxidant activity based on active oxygen scavenging activity and / or radical scavenging activity. Regarding oxidants.

[0002]

2. Description of the Related Art In recent years, active oxygen and radicals in the living body have been attracting attention as factors that oxidize biological components, and their adverse effects on the living body have become a problem. Active oxygen is generated in the process of energy metabolism in living cells, and includes superoxide (ie, superoxide anion (.O _2- ) generated by one-electron reduction of oxygen molecule, hydrogen peroxide (H ₂ O
₂ ), hydroxy radical (.OH), etc. These active oxygens are essential for the bactericidal mechanism of phagocytes and play an important role in the removal of viruses and cancer cells, but excessive production of active oxygen attacks the biomolecules that make up the membranes and tissues in the body. , Induce various diseases. For example, active oxygen is believed to decompose, denature or cross-link biological tissues such as collagen, or to oxidize oils and fats to produce lipid peroxides that damage cells, and these are caused by active oxygen. Is considered to cause aging such as wrinkling of the skin and reduction of elasticity of the skin.

On the other hand, the root part of licorice (licorice root) has been used for a long time as an anti-inflammatory agent, etc., and it has been reported in recent years that it is also effective for cancer treatment. ing. However, the medicinal effect has not been confirmed in the above-ground portion of licorice (for example, licorice leaf portion (licorice leaf)), and it is currently treated as waste because it has no intended use.

[0004]

Therefore, the present invention firstly finds a natural substance having an active oxygen scavenging action and / or a radical scavenging action, and an antioxidant containing the same as an active ingredient. The purpose is to provide. A second object of the present invention is to find utility in the above-ground portion of licorice that is treated as a waste and to effectively utilize licorice.

[0005]

In order to achieve the above object, the antioxidant of the present invention is characterized by containing an extract from the aerial part of licorice as an active ingredient. In the antioxidant of the present invention, the extract preferably has an active oxygen scavenging action and / or a radical scavenging action.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The "extract from the aerial part of licorice", which is the active ingredient of the antioxidant of the present invention, means an extract obtained from the aerial part of licorice, which is obtained from the aerial part of licorice. An extract, a diluted solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or a crudely purified product or a purified product thereof is included.

The licorice aerial part is used as the extraction raw material. The above-ground part of licorice includes leaves, branches, stems, flowers, seeds and the like. Of the above-ground parts of licorice, it is particularly preferable to use the licorice leaf part (licorice leaf) as an extraction raw material. Licorice is a useful plant that belongs to the legume family and has been used as a raw material for medicinal or sweeteners since ancient times. In particular, it has been confirmed that glycyrrhizin and other useful ingredients are contained in the root portion, but these useful ingredients are not contained in the above-ground portion of licorice. On the other hand, the component having an active oxygen scavenging action and / or a radical scavenging action, which was first confirmed by the present inventor, is contained in the above-ground part (particularly licorice leaf) of licorice.

For licorice, Glychyrrhiza glabra, Glychyr
rhiza inflata, Glychyrrhiza uralensis, Glychyrrhiz
a aspera, Glychyrrhiza eurycarpa, Glychyrrhiza pal
lidiflora, Glychyrrhiza yunnanensis, Glychyrrhiza
lepidota, Glychyrrhiza echinata, Glychyrrhiza acan
There are various types such as thocarpa, among these,
The above-ground part of licorice of any kind may be used as an extraction raw material, but it is particularly preferable to use above-ground part of licorice of Glychyrrhiza glabra as an extraction material.

The above-ground part of licorice used as an extraction raw material is
It is suitable to dry and crush immediately after collection. Drying may be performed in the sun or using a commonly used dryer. The above-ground part of licorice may be used as an extraction raw material after pretreatment such as degreasing with a non-polar solvent such as hexane or benzene. By performing a pretreatment such as defatting, it is possible to efficiently perform the extraction treatment of the above-ground portion of licorice with a polar solvent.

In the extraction treatment, it is preferable to use a polar solvent as the extraction solvent. The component having an active oxygen scavenging action and / or a radical scavenging action contained in the aerial part of licorice can be easily extracted by an extraction treatment using a polar solvent as an extraction solvent.

Specific examples of suitable extraction solvents include water, lower aliphatic alcohols, hydrous lower aliphatic alcohols and the like, and these can be used alone or as a mixture of two or more thereof. Specific examples of suitable lower aliphatic alcohols include methanol, ethanol, propanol, 1,3-butylene glycol, glycerin,
Propylene glycol and the like can be mentioned.

Water that can be used as an extraction solvent includes pure water,
In addition to tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, those that have been subjected to various treatments are included. Examples of the treatment applied to water include purification, heating, sterilization, sterilization,
It includes filtration, ion exchange, adjustment of osmotic pressure, buffering, etc. Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

When a mixed solution of two or more polar solvents is used as the extraction solvent, the mixing ratio can be adjusted appropriately. For example, when a mixed liquid of water and lower aliphatic alcohol is used, the mixing ratio of water and lower aliphatic alcohol can be freely changed, but in particular 7: 3 to 2:
8 (weight ratio) is suitable.

The extraction treatment is not particularly limited as long as the soluble component contained in licorice can be eluted in the extraction solvent, and can be carried out by a conventional method. At the time of the extraction treatment, it is not necessary to employ a special extraction method, and any device can be used at room temperature or under reflux heating.

For example, the extraction raw material is put into a treatment tank filled with an extraction solvent, and the soluble components are eluted with stirring occasionally. At this time, the extraction conditions may be appropriately adjusted depending on the extraction raw material and the like, but the extraction solvent amount is usually 5 to 15 times the extraction raw material amount (weight ratio), the extraction time is usually 1 to 4 hours,
The extraction temperature is usually room temperature to 95 ° C.

After the soluble component is eluted by the extraction treatment, the extract residue can be removed by filtration to obtain an extract. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted solution or concentrated solution of the extracted solution, a dried product of the extracted solution, or a crudely purified product or a purified product thereof. You may give a process.

The obtained extract can be used as an antioxidant as it is, but a concentrated solution or a dried product thereof is easier to use. In order to obtain a dried product of the extract, dextrin, in order to improve hygroscopicity,
A carrier such as cyclodextrin may be added.

Further, the extract from the aerial part of licorice does not have an unpleasant odor and does not have the color tone peculiar to the plant extract, so that it can be directly used as a raw material for skin cosmetics, foods and drinks, etc. If necessary, it is also possible to carry out purification for the purpose of activity enhancement, decolorization, deodorization, etc. within a range that does not lead to a decrease in its physiological activity. Specifically, the purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The extract from the aerial part of licorice can be formulated and used according to a conventional method. When formulating,
For facilitating storage and handling, a pharmaceutically acceptable carrier such as dextrin or cyclodextrin or any other auxiliary agent can be added. The extract from the aerial part of licorice can be made into any dosage form such as powder, granules and tablets by formulation.

The antioxidant effect of the extract from the above-ground part of licorice is exerted on the basis of the active oxygen scavenging action and / or radical scavenging action. Here, the "active oxygen" superoxide (· O ₂ -), hydrogen peroxide (H ₂ O _2), contained hydroxy radicals (· OH) and the like. Further, the “radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like. Since the extract from the above-mentioned licorice has an active oxygen scavenging action or a radical scavenging action, it can also be used as an active ingredient of an active oxygen scavenger or a radical scavenger, and the antioxidant of the present invention is Alternatively, these active oxygen scavengers and / or radical scavengers may be contained as active ingredients.

The antioxidant of the present invention effectively prevents the aging phenomenon such as the formation of wrinkles and the decrease in elasticity of the skin by preventing the oxidation of the biological components by the active oxygen scavenging action and / or the radical scavenging action in the living body. -Because it can be treated and has an excellent feeling of use and safety when applied to the skin, it is suitable for incorporation into skin cosmetics.

Skin cosmetics to which the antioxidant of the present invention can be added are not particularly limited, but specific examples thereof include ointments, creams, emulsions, lotions, packs, lips, bath agents,
Hair tonics, hair lotions, soaps, body shampoos and the like can be mentioned.

The blending amount of the antioxidant in the skin cosmetic is
Although it can be appropriately prepared depending on the kind of skin cosmetics, the physiological activity of the extract, etc., a suitable blending ratio is about 0.0001 to 10% by weight in terms of a standard licorice root extract.

The skin cosmetic may contain various main agents and auxiliaries usually used in the production of skin cosmetics, and other optional auxiliaries, as long as they do not hinder the antioxidant action.
Regarding the antioxidative effect of the skin, the antioxidative agent of the present invention is not limited to the main ingredient.

Specific examples of the skin cosmetics which can be used as a constituent together with the antioxidant of the present invention are as follows. When the following constituents are used in combination with the licorice aerial part extract, a synergistic effect between the licorice aerial part extract and the constituents used in combination may bring about an excellent use effect more than normally expected. is there.

Astringent: citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof, aluminum chloride, potassium aluminum sulfate, allantoinchlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenolsulfonate, zinc sulfate, jiu extract, ages. Extract, hamamelis extract, ginger ginger extract, tea catechins, proanthocyanidins,
Gaillard extract, Strawberry extract, Hypericum extract, Rheum palmatum extract, Cornflower extract, Horsetail extract, Kizuta extract, Cucumber extract, Horse chestnut extract, Salvia extract, Melissa extract, Tamarind husk extract, etc.

Sterilizing / antibacterial agents: benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, alkyldiaminoethylglycine chloride liquid, chlorhexidine hydrochloride,
Orthophenylphenol, Photosensitizer No. 101, Photosensitizer 2
No. 01, salicylic acid, sodium salicylate, sorbic acid, halocarban, resorcin, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan degradation product, syrup extract, kujin extract, nematode extract, loquat extract, hop extract, hop extract, Yucca extract,
Aloe extract, cinnamon extract, zedoary extract, oil-soluble licorice extract (licorice hydrophobic flavonoid, glabridin,
Grabrene, Ricochalcone A) etc.

Ultraviolet absorber: β-isopropylfuranone derivative, urocanic acid, ethyl urocanate, oxybenzone, oxybenzone sulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, synoxate, methyl diisopropylcinnamate, octyl methoxycinnamate, paraamino Glyceryl benzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, octyl paraaminobenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, butylmethoxydibenzoylmethane, titanium oxide, β-carotene, γ-oryzanol,
Rice syrup extract, aloe extract, birch extract, birch extract, chamomile extract, black-necked extract, hawthorn extract, henna extract, euglena extract, horse chestnut extract, ginkgo biloba extract, chamomile extract, oil-soluble licorice extract and the like.

Whitening agents: ascorbic acid and its derivatives, sulfur, placenta hydrolysates, ellagic acid and its derivatives, kojic acid and its derivatives, glucosamine and its derivatives, azelei and its derivatives, arbutin and its derivatives, hydroxycinnamic acid and Its derivatives,
Glutathione, Arnica extract, Scutellaria extract, Gypsophila extract, Sophoraceae extract, Psycho-extract, Boufu extract, Hamaboufu extract, Ganoderma lucidum mycelium culture and its extract, Gymnema extract, Shinnaki extract, Peach leaf extract, Agez extract, Kujin extract,
Jew extract, Touki extract, Yokuinin extract, Oyster leaf extract, Rhizoma extract, Button pipi extract, Hamamelis extract, Horse chestnut extract, Hypericum perforatum extract, Odorikosou extract, Oil-soluble licorice extract (liquorice hydrophobic flavone, glabridin, glabrene, Ricochalcone A) and the like.

Moisturizers: serine, glycine, threonine,
Alanine, collagen, hydrolyzed collagen, vitronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipids, sphingolipids, glycosphingolipids,
Linoleic acid or its esters, eicosapentaenoic acid or its esters, pectin, algae colloid, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, Reishi mycelium culture or extract thereof, wheat germ oil, avocado oil, Rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol,
Birodoaoi extract, Yokuinin extract, scented extract, turmeric extract, Kaiso extract, Kidachialoe extract, burdock extract, horse chestnut extract, mannen wax extract, arnica extract, wheat bran, rice bran extract and the like.

Cell activating agents: placenta extract, riboflavin or its derivative, pyridoxine or its derivative, nicotinic acid or its derivative, pantothenic acid or its derivative, α-
Tocopherol or a derivative thereof, arnica extract, carrot extract, ginseng extract, eleuthero extract, loofah extract (saponin), shikon extract, birch extract, ouaba extract, button pi extract, peony extract, swordfish extract, safflower extract, ashitaba extract, loquat leaf extract, Hikiokoshi extract, Yukinoshita extract, yellow mucous extract, salvia extract, garlic extract, mannen wax extract and the like.

Anti-inflammatory / anti-allergic agents: azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or its derivative, glycyrrhetinic acid or its derivative, Photosensitivity No. 301, Photosensitivity No. 401, Hydrochloric acid Diphenhydramine, tranexamic acid or a derivative thereof, adenosine phosphate, estradiol, estrone, ethinyl estradiol, cortisone, hydrocortisone,
Prednisolone, progesterone, corticosterone,
Arnica extract, inchinko extract, sardine extract, sardine extract, licorice extract, Japanese tallow extract, mugwort extract, oleander extract, gentian extract, psychological extract, senkyu extract, sardine extract, perilla extract, laurel extract, perilla extract,
Horse chestnut extract, sardine extract, etc.

Antioxidant / active oxygen scavenger: dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, melissa extract, sardine extract, age extract, loquat leaf. Extract, hop extract, hamamelis extract,
Peony extract, sage extract, kina extract, chamomile extract, eucalyptus extract, perilla extract, ginkgo biloba extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, mace extract, laurel extract, clove extract, turmeric extract, willow extract.

Oils and fats: soybean oil, linseed oil, tung oil, sesame oil, bran oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil, peanut oil, cacao oil, mokurou , Palm oil, palm kernel oil, beef tallow, mink oil, yolk oil, jojoba oil, evening primrose oil,
Horse oil, etc.

Waxes: carnauba wax, candelilla wax, beeswax, honey beeswax, whale wax, cerax, lanolin and the like.

Hydrocarbons: liquid paraffin, petrolatum,
Micro listerin wax, ceresin, squalane, polyethylene powder, etc.

Fatty acids: stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hevenic acid,
Lanolinic acid, oleic acid, undecylenic acid, isostearic acid, etc.

Alcohols: lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol,
Hexadecyl alcohol, 2-octyldodecanol,
Glycerin, sorbitol, propylene glycol, 1,
3-butylene glycol, ethylene glycol or a polymer thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol and the like.

Esters: Decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, propylene glycol dioleate, diethyl phthalate, Propylene glycol monostearate, ethylene glycol monostearate, glyceryl monostearate, glyceryl trimyristate, lanolin acetate, cetyl lactate and the like.

Surfactant: Anionic surfactant, cationic surfactant, amphoteric surfactant, nonionic surfactant and the like.

Fragrances: menthol, carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil and other oily flavors from various animals and plants.

The antioxidant of the present invention can also be incorporated into any food or drink or dietary supplement. In that case, the blending amount should be 1 adult considering the general intake of the food or drink to be added.
The daily intake of licorice ground extract is about 1 to 1000
It is suitable to adjust to mg.

There are no particular restrictions on foods and drinks to which the extract from the above-ground part of licorice is mixed, but specific examples thereof include drinks such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks (these drinks). Concentrated stock solution and adjustment powder); frozen desserts such as ice cream, ice sorbet, shaved ice; noodles such as buckwheat, udon, harusame, gyoza rind, maimai rind, Chinese noodles, instant noodles; candy, chewing gum, candy , Sweets such as gum, chocolate, tablets, snacks, biscuits, jellies, jams, creams, baked goods; processed fish and livestock products such as kamaboko, ham, sausages; dairy products such as processed milk and fermented milk; salad oil, Fats and oils and processed foods such as tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing; sauces Seasonings such as sauce; soup, stew, salad, prepared foods, pickles, and the like.

The antioxidant of the present invention described above is preferably applied to humans, but may also be applied to animals other than humans as long as the respective effects are exhibited. it can.

[0045]

EXAMPLES The present invention will be described in more detail below with reference to examples. The scope of the present invention is not limited to these examples.

Production Example 1 Licorice (Glychyrrhiza glabr
300 g of coarsely crushed a) above-ground part (mainly leaf part, partly including branch part) is put into 2000 mL of extraction solvent (water, 50 wt% ethanol, ethanol or 50 wt% propanol) and loosely added. It was kept at 70 ° C. for 3 hours while stirring. Then, it filtered, the filtrate was concentrated under reduced pressure at 40 degreeC, and also it dried with the reduced pressure dryer, and the licorice ground part extract was obtained. When the above extraction was carried out using four kinds of extraction solvents, the yield of the extract was as shown in Table 1.

[Table 1] Sample No. Extraction Solvent extract yield (wt%) 1 Water 24.0 2 50% Ethanol 21.7 3 Ethanol 12.7 4 50% Propanol 14.3

[Test Example 1] Superoxide erasing test (NBT method) The extract obtained in Production Example 1 was tested for its superoxide erasing action by the following test method.

3 mM xanthine, 0.05M Na ₂ CO
₃ mL of buffer solution (pH 10.2), 0.1 mM each of ₃ mM EDTA, BSA solution and 0.75 mM NBT was placed in a test tube, and 0.1 mL of the sample solution was added to this, and the mixture was allowed to stand at 25 ° C. for 1 hour.
It was left for 0 minutes. Then, a xanthine oxidase solution was added, the mixture was stirred rapidly, and the mixture was allowed to stand at 25 ° C. for 20 minutes. Then add 0.1 mL of 6 mM copper chloride to stop the reaction,
The absorbance at a wavelength of 560 nm was measured. The absorbance measured at this time is referred to as "absorbance upon addition of enzyme solution and sample solution".

Further, the same operation and the measurement of the absorbance were carried out without adding the enzyme solution. The absorbance measured at this time is referred to as "absorbance with no enzyme solution added and sample solution added".

The same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as "absorbance with and without addition of enzyme solution".

Further, the same measurement was carried out when the distilled water was added without adding the enzyme solution and further without adding the sample solution. The absorbance measured at this time is referred to as "absorbance when enzyme solution is not added and sample solution is not added". Then, the superoxide scavenging rate (%) was calculated by the following formula.

Erasure rate (%) = {1- (AB) / (C-
D)} × 100

In the above formula, "A" is "absorbance when enzyme solution is added and sample solution is added", "B" is "absorbance when enzyme solution is not added and sample solution is added", and "C" is "enzyme solution added""Absorptance without addition of sample solution" and "D" represent "Absorptivity without addition of enzyme solution and without addition of sample solution".

The erasing rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the superoxide erasing rate was 50% was determined by the interpolation method. The results are shown in Table 2.

[0056] [Table 2] Sample NO. Extraction was 50% Clear sample concentration 1 water extract 148.0 2 50% ethanol extract 70.6 3 ethanol extract 68.4 4 50% propanol 92.4

From the results shown in Table 2, it was confirmed that the extract from the aerial part of licorice had a superoxide scavenging action. It was also confirmed that the degree of this superoxide scavenging action can be adjusted by the concentration of the extract.

[Test Example 2] Hydrogen Peroxide Elimination Test The extract obtained in Production Example 1 was tested for its action of eliminating hydrogen peroxide by the following test method.

Standard solution of hydrogen peroxide (concentration 1.5 mM)
After adding 10 μL of the sample solution to 10 μL and incubating at 37 ° C. for 20 minutes, the coloring reagent [DA-64 (manufactured by Wako Pure Chemical Industries, Ltd.) was 10 mM, Triton X-100 was 0.5.
% Of 0.1 M PIPES buffer (pH 7.0) containing 1 mL of peroxidase solution (100 unit / mL, manufactured by Wako Pure Chemical Industries, Ltd., adjusted to 100 mL in total) 2.98 mL was added, and the mixture was added at 5 ° C. at 37 ° C. Incubated for minutes. Then, the light absorbency in wavelength 727nm was measured. The absorbance measured at this time is called "absorbance when adding the hydrogen peroxide standard solution and the sample solution".

Further, the same operation and the measurement of the absorbance were carried out without adding the hydrogen peroxide standard solution. The absorbance measured at this time is referred to as "absorbance without addition of hydrogen peroxide standard solution and with addition of sample solution".

The same measurement was carried out when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as "absorbance when the hydrogen peroxide standard solution is added and the sample solution is not added".

Further, the same measurement was carried out when the standard solution of hydrogen peroxide was not added and the distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as "absorbance without addition of hydrogen peroxide standard solution and without addition of sample solution". Then, the hydrogen peroxide elimination rate (%) was calculated by the following formula.

Erasure rate (%) = {1- (AB) / (C-
D)} × 100

In the above formula, "A" is "absorbance when adding hydrogen peroxide standard solution and sample solution", "B" is "absorbance when no hydrogen peroxide standard solution is added and sample solution", "C""
Is the "absorbance when the hydrogen peroxide standard solution is added and the sample solution is not added", and "D" is the "absorbance when the hydrogen peroxide standard solution is not added and the sample solution is not added".

The erasure rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the hydrogen peroxide erasure rate was 50% was determined by the interpolation method. The results are shown in Table 3.

[0066] [Table 3] Sample NO. Extraction was 50% Clear sample concentration 1 water extract 46.7 2 50% ethanol extract 37.3 3 ethanol extract 42.5 4 50% propanol 56.7

From the results shown in Table 3, it was confirmed that the extract from the above-ground part of licorice has a hydrogen peroxide scavenging action. It was also confirmed that the degree of this hydrogen peroxide scavenging action can be adjusted by the concentration of the extract.

Test Example 3 Radical Scavenging Test The extract obtained in Production Example 1 was tested for radical scavenging action using DPPH, which is a very stable radical, by the following test method.

3 mL of the sample solution was added to 3 mL of 1.5 × 10 ⁻⁴ M DPPH ethanol solution, the container was immediately sealed and shaken, and the mixture was left standing for 30 minutes. Then wavelength 520
Absorbance at nm was measured. As a control
The same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, 3 mL of the sample solution was added to ethanol, and immediately thereafter, the absorbance at a wavelength of 520 nm was measured. The radical scavenging rate (%) was calculated by the following formula from each measured absorbance.

Radical scavenging rate (%) = {1- (BC)
/ A} × 100

In the above formula, "A" is "absorbance of control", "B" is "absorbance of sample solution added",
"C" represents "blank absorbance".

The scavenging rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the scavenging rate of DPPH radical was 50% was determined by the interpolation method. The results are shown in Table 4.

[0073] [Table 4] Sample NO. Extraction was 50% Clear sample concentration 1 water extract 230.0 2 50% ethanol extract 119.7 3 ethanol extract 140.1 4 50% propanol 160.3

From the results shown in Table 4, it was confirmed that the extract from the aerial part of licorice has a DPPH radical scavenging action. It was also confirmed that the degree of this DPPH radical scavenging action can be adjusted by the concentration of the extract.

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method. Jojoba oil 4.0 g Placenta extract 0.1 g Magnesium ascorbyl phosphate 0.1 g Prune extract 1.0 g Tonin extract 1.0 g Kyounin extract 1.0 g Taiou extract 0.5 g Kujin extract 0.5 g Olive oil 2.0 g squalane 2.0 g cetanol 2.0 g glyceryl monostearate 2.0 g polyoxyethylene cetyl ether (20E.0) 2.5 g polyoxyethylene sorbitan oleate (20E.0) 2.0 g 1,3-butylene Glycol 3.0 g Hinokitiol 0.15 g Perfume 0.15 g Licorice ground part water extract 10.0 g Purified water balance (total amount is 100 g)

[Formulation Example 2] A cream having the following composition was produced by a conventional method. Liquid paraffin 5.0 g Salix beeswax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Arbutin 0.1 g Rucinol 0.1 g Kojic acid 0.1 g Yellow mud extract 0.1 g Ginkgo biloba extract 0.1 g Conchiolin 0.1. 1 g Oat extract 0.1 g Stearic acid 1.0 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3.0 g 1,3-Butylene glycol 6.0 g Methyl paraoxybenzoate 0.05 g Perfume 0 1 g 50% ethanol extract of licorice above ground 5.0 g Purified water balance (total amount is 100 g)

[Formulation Example 3] A pack having the following composition was produced by a conventional method. Polyvinyl alcohol 15.0 g Polyethylene glycol 3.0 g Propylene glycol 7.0 g Ethanol 10.0 g Chamomile extract 0.1 g Ellagic acid 0.1 g Oil-soluble licorice extract 0.1 g Seaweed extract 0.1 g Yeast extract 0.1 g Perilla extract Liquid 0.1 g Ju extract 0.1 g Linden extract 0.1 g Ethyl paraoxybenzoate 0.05 g Perfume 0.05 g Licorice ground part ethanol extract 3.0 g Purified water balance (total amount is 100 g)

[Formulation Example 4] A capsule having the following composition was produced by a conventional method. No. 1 hard gelatin capsule was used as the capsule. Licorice ground part water extract 20.0 mg Corn starch 60.0 mg Lactose 100.0 mg Calcium lactate 10.0 mg Hydroxypropyl cellulose (HPC-L) 10.0 mg

[Formulation Example 5] A granular preparation having the following composition was produced by a conventional method. Licorice 50% ethanol extract 50.0 mg Beef bone powder (containing 20% calcium) 250.0 mg Eggshell calcium (containing 34% calcium) 147.0 mg Soybean extract (containing 10% isoflavone) 25.0 mg Vitamin B group mixed powder (Including all vitamin B groups) 100.0 mg Fat-soluble vitamin mixed powder (including vitamins D, K, E and A) 100.0 mg Milk protein hydrolyzate (including amino acids and low molecular weight peptides) 50.0 mg Corn Soy protein hydrolyzate (including amino acids and peptides) 20.0 mg Wheat germ extract (including vitamins, chromium, selenium and molybdenum) 30.0 mg Yeast extract (including vitamins, nucleic acids and zinc) 50.0 mg Erythritol 300. 0 mg lactulose 100.0 mg maltitol 168 7mg beet oligosaccharides 100.0mg Chichihate oligosaccharide 100.0mg guar gum 60.0mg glycerin fatty acid ester 15.0mg

[Formulation Example 6] An oral liquid preparation having the following composition was produced by a conventional method. Licorice ground ethanol extract 0.5 g sorbit 12.0 g sodium benzoate 0.1 g fragrance 1.0 g calcium sulfate 0.5 g purified water balance (total amount is 100 g)

[0081]

INDUSTRIAL APPLICABILITY The present invention provides a novel antioxidant. According to the antioxidant of the present invention, aging phenomena such as formation of wrinkles of the skin and reduction of elasticity are effectively prevented by preventing oxidation of biological components due to active oxygen scavenging action and / or in vivo radical scavenging action. Can be treated. In addition to the above effects, the antioxidant of the present invention has excellent usability and safety when applied to the skin, and is therefore suitable for incorporation in skin cosmetics and foods and drinks.

   ─────────────────────────────────────────────────── ─── Continued front page    F-term (reference) 4C083 AA072 AA111 AA112 AA122                       AC022 AC072 AC102 AC122                       AC182 AC242 AC422 AC442                       AC482 AC842 AD042 AD112                       AD412 AD512 AD552 AD642                       BB47 CC05 CC07 DD31 EE09                       EE12 EE16                 4C088 AB60 AC02 BA08 NA14 ZA89                 4H025 BA01

Claims (2)

[Claims] 1. An antioxidant comprising an extract of licorice from the aerial part as an active ingredient. 2. The extract has an active oxygen scavenging action and / or
Alternatively, the antioxidant according to claim 1, which has a radical scavenging action.