JP2002059131A - Control material for soil microbe-carrying water content and manufacturing method thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To stably provide a water content control material capable of keeping the fermentation time and the fermentation temperature of microorganisms properly by using the water content control material formed by carrying the soil microbe on a cellulose based material to perform the moisture control of a material to be fermented at the time of fermenting human waste, fish refuse, metal refuse, vegetable waste, kitchen waste and the like or dried food residue having small water content or bread waste to reduce volume. SOLUTION: The water content control material is formed by carrying the soil microbe on natural cellulose provided with fine pores, particularly flow out fine pulp residue or sludge generated in a paper making/pulp plant. The water content control material is formed by carrying Bacillus Coagulans and/or Bacillus Circulans (Agency of Science and Technology Biotechnology Laboratory FERM P-17807). The water content control material is treated by heating. The manufacturing method of the soil microbe-carrying moisture control material is provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a fermentation process for excrement of manure, fish residue, meat residue, vegetable waste, kitchen waste, or the like containing a large amount of water, or processed food residue or bread waste containing a small amount of water. The present invention relates to a moisture-regulating material that carries cells for regulating the moisture and the species of a fermented product, and relates to the field of microbial materials for agriculture, fisheries, and livestock.

[0002]

2. Description of the Related Art With regard to fine pulp residues discharged from paper and pulp mills (hereinafter referred to as pulp residues), the soil improvement effect of cellulose fiber has been attracting attention.
On the other hand, when performing fermentation treatment of manure containing a large amount of water, there is a method of adjusting the water suitable for fermentation by using rice husks, bushes, rice bran, straw, or the like, for example, a nozumi fermentation. However, fermentation treatment of organic matter, especially lignin, etc. contained in the added rice hulls, bush, rice bran, straw and the like requires a long time, and as a result, rapid fermentation treatment of manure and the like has not been realized.

[0003] With respect to this problem, a method of shortening the fermentation treatment time by using an inorganic dried product as a moisture control material has been proposed, and is partially used as a moisture control material. However, when the inorganic dried product is used as a moisture control material, careful fermentation treatment management including a formulation is required to obtain sufficient fermentation treatment temperature and time.

Further, in order to obtain a dried inorganic product, it is necessary to carry out heat incineration treatment and the like, and at the same time, additional use of the inorganic material does not contribute to volume reduction of the finished fermentation product.

[0005] On the other hand, materials that require large amounts of water, such as manure, have reached a considerable amount, and fermentation reduction treatment of food residues has been desired. The fermentation volume reduction treatment using a moisture control material not only minimizes the amount of fuel used, but also has the convenience of not requiring excessive wastewater treatment. Development related to stable supply is desired.

[0006] As a fermentation aid, a microbial material containing an enzyme is on the market. However, in general, the content of the composition has not been clarified, and the development of a rational combination technique with natural cellulose materials, particularly pulp residues, has been desired. In the current situation where a recycling-oriented society is being sought and legislative development is underway, the development of a rational fermentation treatment method is attracting attention, but the development of a moisture control material as a microbial material that meets such demands Has not been done.

[0007]

SUMMARY OF THE INVENTION An object of the present invention is to provide a large amount of water-containing excrement, fish scale, meat scale, vegetable waste, kitchen waste, and the like.
In addition, when fermenting processed food residues or bread crumbs that do not contain much water, the fermentation treatment of lignin etc. does not take a long time, does not require excessive wastewater treatment, and reduces the volume of the finished fermentation product. It is an object of the present invention to provide a soil bacteria-carrying moisture control material which is an inexpensive and easy-to-handle treatment material, and a method for producing the same.

[Means for Solving the Problems]

The invention according to claim 1 is characterized in that a dehydrated cake containing water having a water content of 45 to 80% by weight and containing natural cellulose having micropores is mixed with Bacillus coagulans.
Coagulans) and / or Bacillus Circulans (FERM P-17807)
A soil bacteria-carrying moisture regulating material characterized by carrying soil bacteria.

It is necessary that the support for supporting the microbial cells be an excellent material also as a moisture control material for the material to be fermented. The use of a cellulosic material as this natural material-based moisture control material can be generally assumed. However, the present invention is characterized by using a dehydrated cake containing natural cellulose having micropores. In the fermentation decomposition treatment, the indicator for supporting the bacterium can be easily decomposed, that is, it is fine and has many pores, and the cellulosic body is preferably damaged and can be stably supplied in a large amount. Natural cellulose with micropores is necessary and optimal.

As the natural cellulose having such fine pores, for example, a fine pulp residue discharged from a papermaking and pulp mill is particularly preferable. This pulp residue has fine pulp fibers, and at the same time, most of the lignin contained in the raw wood is separated from the pulp fibers, and is strongly damaged by the mechanical damage. In addition, countless pores are formed. Therefore, it is easily affected by soil microorganisms and is easily used as a bacterial carrier.
These pores can also be used as a space for supporting bacterial cells. Further, it is a material capable of stably supplying pulp residues, and can be used as a moisture control material. In the present invention, it is important that natural cellulose having such micropores is contained, and even if some impurities are contained, the effect of the present invention is not impaired.

[0011] Decomposition of the cellulosic material constituting the moisture control material of the present invention by soil microorganisms is also important from the viewpoint of volume reduction. An environment in which the cellulose body is decomposed by soil microorganisms requires a high temperature environment, a certain degree of acidity, and a nutrient source for decomposing bacteria. In the present invention, as long as the cellulose body is used for controlling the moisture content of the fermented product,
As a starting nutrient source for the degrading bacteria, it will be replenished from the water-regulated material, and consequently the acidity will also be regulated. However,
It is also possible that low pH values are reached.

[0012] Therefore, as the characteristics of the soil microorganisms to be employed, it is preferable that heat shock effectively acts on the microorganisms, and that the microorganisms also act on the microorganisms even at a low pH value. Is preferably purified.

In the present invention, spore-forming bacteria, particularly soil bacteria, Bacillus. Coagulans and / or Bacillus. Circulans (hereafter,
B. Coagulans, B .; Circulans cells. The cells are carried on natural cellulose having micropores, especially pulp residue, by focusing on the action on cellulose in a high temperature region and the heat resistance and PH characteristics of spores. There are a large number of non-spore-forming bacteria in nature as thermophilic bacteria, but these soil bacteria were selected because of their spore-forming bacteria in view of their durability against the high temperature region, which is a cellulose degradation environment, and Gram stain characteristics. Utilization is advantageous, and aerobic bacteria are preferred.

There are many harmful microorganisms in microorganisms that live in nature regardless of bacteria. Bacillu
s genus is no exception, and Bacillus. An
thracis is famous. On the other hand, useful microbial cell utilization technology is also accumulated. Bacillus. Subtil
Use of is as a natto fungus and use as a bacterium for wastewater treatment can be mentioned.

Although the use of these cells is not effective and theoretically utilized up to the spore-forming function and the germination mechanism, it is necessary to specify the cells before use. Suggestive. In the present invention, B.I. Coagulans cells and B. Circula
Both the ns microbial cells and the natural cell having micropores may be supported, or either one of them may be supported.

At the time of decomposing fats and oils, B.I. Subtilis is available.
The present invention relates to Subtilis is not limited by the combined use. Coagulans, B .; Cir
culans cells are essential.

Most water used in paper and pulp mills depends on river water. Therefore,
Many of the microorganisms from river water are present in the process water of the mill and consequently adhere to the effluent fine pulp. In the case of containing groundwater, microorganisms derived from the groundwater also attach.
Further, the same applies to microorganisms derived from the air or materials used.

The present invention is not limited by the composition of these microflora. Coagulans,
B. At least one of Circulans cells is essential. Then, the concentration of the cells is increased or the cells are surely supported, and further, the preservation of the moisture control material enables fermentation in a wide area.

B. Coagulans, B .; Circu
Lans cells are inoculated into the dehydrated cake. When inoculating the cells before the dehydration treatment, mixing of the cells is easy, but the cells inevitably flow out in a considerable amount, which increases the cost of the cells and promotes the generation of slime in the reused clear water pipes. It is. It is preferable that the degree of the dehydration treatment be the moisture that can be handled by the dehydrated cake. When the water content of the dewatered cake is dehydrated to less than 45%, it becomes a solid mass, which hinders subsequent processing, for example, stirring and mixing processing or particle size adjustment, resulting in an increase in costs for crushing equipment and power.

If the water content of the dewatered cake exceeds 80%, it will hinder the conveyance of the belt conveyor and the like, and the fuel cost will increase in the subsequent heat treatment and the like. Therefore, as shown in (Example 1), the amount of water that can be handled as a lump, that is, the amount of water is adjusted to 45 to 80% by weight,
It is preferably used as a raw material. As the shape of the moisture control material, it can be in the form of cotton or granular, lump, rod, or clay,
The invention is not limited to these shapes.

As described above, the dewatered cake composed of pulp residue and the like is treated with B.I. Coagulans, B .; Circulan
s The method of constructing a moisture control material by stirring and mixing the cells and inoculating the cells is simple, and the size of the moisture control material can be adjusted at the stage of stirring and mixing, which is reasonable. . However, since the moisture control material according to the present method has a high moisture content of 45% or more, the storage period of the moisture control material is 2%.
About 3 days or less. When stored for longer than this period, mold generation has been confirmed, depending on the storage environment conditions.

Therefore, when storing water at 45% or more, it is necessary to take measures such as vacuum packaging, but this is not preferable from the practical point of view such as an increase in the amount of work or an increase in cost. Rather, storage in the sun and in the air is reasonable. Furthermore,
A combination of heat treatment and solar / air drying treatment is also effective.

As described above, the soil-bacteria-supported water-regulating material of the present invention can be used as a water-regulating material by controlling natural cellulose having micropores, particularly pulp residue, in an economical manner while sufficiently utilizing the characteristics of microorganisms. It can be used for a wide range of fermentation treatments.

The invention according to claim 2 is characterized in that the final water content containing natural cellulose with micropores exceeds 15% by weight.
A soil-bacteria-supported water-regulating material, wherein the soil bacterium is carried on a dewatered cake of less than% by weight.

The production method of the present invention includes, for example,
3. The method for producing a soil-bacteria-supported moisture-regulating material according to 3, 4, or 5, further including a method of adjusting the final moisture of the soil-bacteria-supported moisture-regulating material to more than 15% by weight and less than 45% by weight. According to the present invention, in particular, it is possible to ensure the preservability of the moisture control material and to enable the fermentation treatment in a wide area.

As shown in (Embodiment 10), Coa
gulans, B .; The growth of Circulans cells has been confirmed at 55 ° C. or higher and 80 ° C., and the spores have been subjected to heat shock. However, when the final moisture content of the material to be heated is high, it approaches the state before heating due to various bacteria in the environment. Therefore, a method of simultaneously drying the material to be heated is appropriate.
It should be noted that non-dry storage such as vacuum packaging is not practically suitable but is not limited.

When the heating and drying treatment is carried out at a temperature of 55 ° C. or more and 80 ° C. or less as the material temperature of the material to be heated, water having almost no biological activity, ie, final water 15
%, It takes a long time. However, when the final moisture content of the soil bacteria-carrying moisture control material is adjusted to more than 15% by weight and less than 45% by weight, the damage caused by microbial contamination is improved and the preservability is improved. More preferably, when the final water content is adjusted to more than 15% by weight and not more than 30% by weight, as compared with the case where the final water content exceeds 30% by weight, damage caused by microbial contamination is improved and storage stability is improved.

When the moisture control material having the final moisture is handled as a local material, the material has sufficient storage stability, and furthermore, has a lower moisture absorption speed than the case where the final moisture is 15% or less. Excellent. When the fermentation treatment is performed using the moisture control material, almost the same results can be obtained as compared with the fermentation treatment using the moisture control material of the present invention without heat treatment.

The invention according to claim 3 is characterized in that the soil bacteria are supported on a dehydrated cake containing natural cellulose having micropores and having a final moisture content of 3 to 15% by weight. Material.

The production method of the present invention includes, for example,
3. The method for producing a water-regulating material carrying soil bacteria according to 3, 4, or 5, further comprising adjusting the final moisture of the water-regulating material carrying soil microorganisms to 3 to 15% by weight. According to the present invention, in particular, it is possible to ensure sufficient storage stability of the moisture control material, and to enable fermentation treatment in a wider area.

In the moisture range of more than 15% by weight and less than 45% by weight, depending on the storage environment conditions, storage for more than one week induces mold generation and the like. Therefore, it is preferable that the final moisture of the moisture control material which requires further long-term storage be 15% or less. The minimum moisture content is the minimum air-dried moisture, and the minimum air-dried moisture of the moisture control material of the present invention is 3%. When the final moisture content is adjusted to less than 3%, it becomes 3% or more due to natural moisture absorption. Therefore, especially when long-term storage is required, it is preferable that the final moisture content of the moisture control material according to the present invention is adjusted to 3 to 15% by weight.

B. supported on the moisture control material according to the present invention prepared to have a final moisture content of 3 to 15% by weight. Coagula
ns, B.S. The Circulans cells need to grow or germinate when the moisture control material is used for processing. During the heat treatment, the temperature of the material to be heated is set to 5
Treated in the range of 5 ° C or more and 80 ° C or less, and the final moisture was 3%
When the content is set to １５15% by weight, the instantaneous moisture absorption performance is reduced, but almost the same result is obtained as compared with the fermentation treatment using the moisture control material according to the present invention in which the heating and drying treatment is not performed.

[0033] The invention according to claim 4 is characterized in that the fine pulp residue or sludge discharged from a papermaking and pulp mill has a water content of 45 to 45%.
A dehydrated cake adjusted to 80% by weight was added to the dehydrated cake, and Bacillus Coagrance was added to the dehydrated cake.
urans) and / or Bacillus Circulans (FERM P-17807, National Institute of Bioscience and Biotechnology). Is a manufacturing method.

According to the production method of the present invention, the soil-bacteria-supported moisture control material according to the first aspect can be produced. In the present invention, B.I. Coagulans, B .; Circula
ns cells are inoculated into a dehydrated cake.
When inoculating the cells before the dehydration treatment, mixing of the cells is easy, but the cells inevitably flow out in a considerable amount, which increases the cost of the cells and promotes the generation of slime in the reused clear water pipes. It is. In addition, use the fine pulp residue or sludge discharged from the paper and pulp mill, and the water content is 45 to 80% by weight.
And Bacillus Coagul
ans and / or Bacillus Circula
The significance of using the soil bacteria ns is the same as in the first aspect of the present invention.

According to a fifth aspect of the present invention, there is provided the method for producing a soil bacteria-supported moisture control material according to the fourth aspect, further comprising:
A method for producing a soil-bacteria-carrying moisture-regulating material, characterized in that the material is subjected to a heat treatment at a temperature of ° C.

The water-regulated material inherently contains various germs. Therefore, forcible heat sterilization is required at any stage of the moisture-regulating fermentation in order to kill various germs, rather than the heat treatment which is performed supplementarily to the fermentation. Regarding the heat treatment method, depending on the individual use method, furthermore, a rational means method according to the purpose will be adopted, but it may be reasonable to perform the heat treatment at the stage of producing the moisture control material. .

Further, there is a case where the improvement of the preservability of the moisture control material by the heat treatment is also an important subject. When performing the heat treatment in this manner, the heating temperature range is defined, the final moisture content is adjusted according to the purpose, and the moisture adjusting material is adjusted.

B. Coagulans, B .; Circu
When the purpose is to increase the concentration of lans cells, the water content of the dehydrated cake is adjusted according to the purpose, the cells are inoculated, and then heat treatment is performed to achieve the purpose by using a moisture control material. be able to. In performing the heat treatment, the material to be heated is heated to 55 ° C. or higher, so that B.I. Coagulans, B .; Circulans
Many of the resident bacteria other than the cells can be killed.

According to a sixth aspect of the present invention, in the method for producing a soil bacteria-carrying moisture regulating material according to the fourth aspect, the heat treatment is further performed at a temperature of more than 80 ° C. and 250 ° C. or less, and after cooling to 80 ° C. or less,
A method for producing a soil-bacteria-supported moisture regulating material, comprising inoculating the soil bacterium and supporting the soil bacterium.

For example, the final moisture of the moisture control material is 15%
When the temperature is set to be lower than 80 ° C. or less, it takes a long time. It is effective to increase the heating temperature in order to shorten the heating time and reduce the capital investment cost and the repair cost. However, the upper limit of the heating temperature of the material to be heated is the thermal breakdown temperature of the fine pulp raw material used as the natural cellulose material, that is, 250 ° C.

The temperature of the material to be heated exceeds 80 ° C. and 25
When processing at 0 ° C. or lower, B. Coagulans,
B. A preferred method of inoculating Circulans cells is as follows.

The fine pulp raw material used as a natural cellulosic material has most of the lignin separated, and is further strongly damaged by mechanical damage. However, cellulose decomposition fermentation by microorganisms requires some time. Therefore, in order to shorten the cellulose decomposition fermentation time, it is necessary to have a high temperature. Therefore, the dehydrated cake adjusted to have a water content of 45% or more and 80% or less has B.I. Coag
ulans, B .; The time required for inoculating the Circulans cells and subsequent heat treatment until the cells are killed by heating is a very effective time from the viewpoint of promoting cellulose degradation, that is, supplying nutrients such as inoculated microorganisms. .

It is well known that cellulose has excellent heat stability performance. Therefore, the temperature of the material to be heated is 2
When heated to 50 ° C. as an upper limit, the spores carried in the pores of the cellulosic body have died to some extent, and the cells have been damaged by heat. Therefore, B.I. C
oagulans, B.A. Circulans cells were inoculated and heat-treated at a temperature of more than 80 ° C. and 250 ° C. or less. During the subsequent cooling process, the temperature of the material to be heated was 80 ° C. or less. Coagulans, B .; Circ
Inoculation with B. ulans cells ensures B. ulans cells. Co
Agulans, B.A. Circulans cells can be carried.

According to this method, even if the final moisture content of the moisture control material is 3 to 15% by weight, B.I. Coagula
ns, B.S. Since the Circulans cells are re-inoculated,
Microorganism treatment performance equivalent to that of the above-mentioned moisture control material can be exhibited.

In the present invention, the temperature of the material to be heated exceeds 80 ° C.
Heat treatment in a temperature range of 50 ° C. or less has disadvantages such as fuel cost and addition of inoculation of cells. However, by setting the final moisture of the moisture control material to 15% or less, excellent preservability and wide range Can be moved and stored.

According to a seventh aspect of the present invention, the dehydrated cake according to the fourth aspect is heated at 55 to 250 ° C., cooled to 80 ° C. or lower, and then inoculated with the soil bacteria to carry the soil bacteria. A method for producing a soil bacteria-carrying moisture control material, characterized in that:

By rapidly heating the dehydrated cake,
The odor attached to the pulp residue, for example, the anaerobic fermentation smell after activated sludge treatment can be reduced in a short time.
Activated sludge treated products are promising as a natural cellulose material supply source, but anaerobic fermentation proceeds in a short time if left untreated,
This produces a characteristic odor. Therefore, incineration is generally performed promptly, but according to the present invention, it can be treated almost odorless, and can be effectively used as a natural cellulose material supply source.

Further, for example, when the purpose of the treatment is cattle dung and the time required for fermentation of the fermented product itself is long, the dehydrated cake is not inoculated with the cells.
The method of inoculating the cells after the completion of the heat treatment is an effective form because the operation steps are simplified.

In the present invention, the material to be heated is
Heat treatment in a temperature range of more than 80 ° C. and 250 ° C. or less has disadvantages such as fuel cost. However, by setting the final moisture of the moisture control material to 15% or less, the storage stability is excellent, and the water content is excellent. Can be moved and stored.

BEST MODE FOR CARRYING OUT THE INVENTION

The moisture control material of the present invention comprises Coagu
lans, B .; It is composed of Circulans cells and pulp residue, and heat-treated if necessary. The use of solar air drying is also effective. B. coagulans, B.A. c
As an irculans cell, "SURUGA (Suruga)
-001A "and / or" SURUGA (Suruga) -0 "
01B "(FERM Biotechnology Industrial Technology Research Institute FERM
P-17807) is used. Since the cells have an excellent purification level and the species identification is clarified, enormous knowledge concerning them can be used.
Therefore, its use is an effective form for completing the present invention.

As the pulp residue, fine pulp residue generated from the kraft pulp manufacturing process, the ground pulp manufacturing process, the waste paper pulp manufacturing process, and the papermaking process can be used. These pulp residues are used in the pulp manufacturing process,
It is recovered as a dewatered cake through coagulation treatment and dehydration treatment as a flow-down product passing through a wire mesh in a pulp extracting step and a paper extracting step. On the other hand, a part of the clear water is reused.

The cellulosic material is discharged from other than the above steps. For example, Jansson's knot slag, objects dropped on a conveyor, yard temporary storages, etc. can be mentioned, but the present invention is not limited to the mixed use of these, etc.
The use of pulp residues generated in a pulp mill is a constituent requirement.

A dehydrated cake whose water content has been adjusted by a dehydration treatment and B.I. Coagulans, B .; Circulans cells are mixed with a stirring mixer, and the cells are inoculated. The present invention is not limited to a stirring and mixing system, a heating system, or a heating and drying system, but as an example, a horizontal stirring mixer, a drum rotary stirrer represented by a kiln, a stirring blade rotary type drum fixed type. A stirrer, a combination stirrer, a kneader and the like can be used. As the vertical stirring mixer, a stirring blade rotating type drum fixed type, a type with a smearing mechanism and the like can be used.

As the heating equipment, a direct heat heating type, an indirect heating type, an air flow type, a vibration type, an electric heating type, a high frequency heating type, a far infrared heating type can be used, and a device provided with stirring, mixing and drying is of course also available. Can be used. The present invention is not limited to these facilities.

As shown in (Example 2), a considerable amount of microorganisms can be carried on the dehydrated cake. However, as a measuring method, the number of colonies generated on an agar medium by a dilution method is counted, and it is highly possible that the number of spores is counted, but it can be evaluated that the number is almost the same as that of general soil.
It is well known that spores and water are well-adapted, and it can be said that the water retained in the dehydrated cake is sufficient to support spores.

As a method for drying the dehydrated cake, solar drying and air drying are also effective. Fine cellulose pulp requires a significant amount of fuel to dry. Therefore, the final moisture can be reduced to 3% by drying in the sun or in a greenhouse.
And less than 45%. 45% final moisture
When it is prepared so as to be less than the above, the moisture adjusting material becomes a solid mass as shown in (Example 1), and the storage and handling of the finished product are remarkably easy.

In the case where the moisture control material is subjected to a sun / air drying treatment, it is possible to dry the moisture control material while reducing damage to microbial contamination by reducing the spray thickness. Therefore, the water content can be adjusted to 3% only by sunlight and air drying. The method used in combination as a pre-treatment or a post-treatment of the heat treatment is also excellent in economy. Further, the temperature of the material to be heated is set to 80 ° C.
It is also effective to use it as a pretreatment or a posttreatment in the step of cooling and inoculating the cells below. However, the present invention does not stipulate that a solar-air-drying process is incorporated, and is not limited to the present invention, and is not limited by equipment or jigs and tools.

In the present invention, water is expressed as a percentage by weight obtained by dividing the weight of the contained water by the actual weight of the material.

[0059]

[Examples] As a dewatered cake sample, wastewater generated from the papermaking process is collected by coagulation sedimentation, and a dewatered cake obtained by dewatering, wastewater deinking process wastewater and mixed wastewater of the papermaking process wastewater are treated with activated sludge and then dewatered. After treating the treated dewatered cake, kraft pulp manufacturing process wastewater, ground pulp manufacturing process wastewater and papermaking process wastewater with activated sludge,
Three types of dewatered cakes were used.

(Example 1) Handling properties of dewatered cakes As to the three kinds of dehydrated cake samples, the water content was changed and the easiness of handling was evaluated. Within this range, it can be handled as a raw material for a moisture control material. Table 1 shows the results.

【table 1】

(Example 2) Amount of bacterial cells supported on dehydrated cake Samples No-, No-, and No- were added to sterile water, mixed by stirring for 10 minutes with a reciprocating shaker, and diluted by a 10-fold dilution method. Then, colonies were formed in YG medium and counted. In each sample, the number was 10 ⁶ to 10 ⁷ per 1 g absolute dry weight.

Example 3 Bacterial Cell Carrying Amount of Dry Dehydrated Cake Samples No-, No-, and No- were formed into a diameter of 5 mm and a length of 10 mm by a desk-type granulator, and dried to 3% moisture. Then, steam heat sterilization treatment was performed at 121 ° C. for 30 minutes to obtain a sample. The cells were sprinkled on the sample, washed off with a shaker, and counted according to (Example 2). The results were ¹⁰ 3 to 10 ⁴ for any of the samples.

Example 4 Chicken Manure Fermentation Treatment For the purpose of chicken manure treatment, especially when collected as broiler manure, the water content is generally 50% or less. Therefore, the water content of the fine pulp dewatered cake discharged in the papermaking process is adjusted to a range of 60% or more and 80% or less,
B. Coagulans, B .; The Circulans cells are mixed with stirring and inoculated. Or B. Coa
gulans, B .; By stirring and mixing the Circulans cells and then adjusting the water content to a range of 60% or more and 80% or less, a water-regulating material suitable for treatment can be obtained.

Broiler manure is discharged in a relatively dry state as the poultry house topsoil after completion of fattening, and chicken manure itself is widely used as an auxiliary for fermentation, but its odor has some problems. Therefore, the initial odor can be improved at the same time by adjusting the moisture content of the moisture adjusting material to a higher level.

In this example, the dehydrated cake sample No.
B. Coagulans, B .; Circulans
The number of cells was calculated as (1
/ 5000) × (10 ⁵ to 10 ⁸ ), and the mixture was stirred and mixed at room temperature for 4 hours to prepare a moisture control material. This water-regulating material was mixed and stirred at the same weight ratio as the broiler feces, the water content of the mixture was adjusted to 55 ± 5%, and fermentation treatment was performed for 14 days. The fermentation temperature exceeded 70 ° C. after 3 days, and the fermented product was almost odorless and had a water content of 45 ± 5%.

(Example 5) Cow manure fermentation treatment For the purpose of cattle manure treatment, the manure water content is high, about 90%. Therefore, the water content of the dewatered cake is preferably adjusted to be in the range of 45% to 55%. B. Coagulans, B .; Circul
The method of inoculating ans cells is the same as described above, and can be used as a water-regulating material for treatment.

Cow manure can be roughly classified into dairy cows and fattening cows. As a common feature of the manure, its water content is considerably higher than that of other livestock manure, and although it is a substance that is difficult to ferment, it hardly generates an odor when it is discharged. is there. The odor is presumed to be largely due to roughage, but storage under anaerobic conditions causes odor.

Therefore, composting can be made almost odorless by quickly adjusting the moisture content with the moisture adjusting material according to the present invention. Dehydrated cake sample
No- was used, and the moisture control material prepared in Example 4 was used to adjust the water content of cow manure according to the formulation shown in Table 2, and a fermentation treatment was performed for 60 days. As the cow manure, manure generated from a tethered barn of Japanese Black cattle was used, and switching was performed once after 30 days of accumulation. The fermentation temperature and the presence or absence of odor generation were observed without nitrogen supplementation, and the results are shown in FIG.

[0069]

[Table 2] In addition, Example 5-1 of FIG. 1, 5-2, 5-3, 5-
The mixing weight ratio of the cow manure and the moisture control material used was
See Table 2. The fermentation attainment temperature is slightly lower, but can be sufficiently coped with an increase in the amount of sedimentation, combined use of chicken manure, or forced heating for several days.

FIG. 1 shows that a cow manure fermentation treatment which conventionally required a sedimentation for more than six months can be completed in several months. In addition, when the manure contains a large amount of rice husks, basins, sawdust, etc., it is preferable to use chicken manure as a forced heating or fermentation aid. When these are not included, fermentation treatment is possible only with the moisture control material according to the present invention. Further, in the case of collectively processing cow excrement discharged in large quantities, it is also effective to reduce the weight of the moisture control material by drying.

(Example 6) Fermentation treatment of pig manure The water content of pig manure is in the range of water between chicken manure and cow manure, but there is also a problem of water for cleaning pigs, and the water content of the dehydrated cake is 45% to 65%. It is preferable that the dehydration is adjusted in the range of. Pig manure compost has traditionally left some odor problems. The fermentation-treated product using the moisture control material according to the present invention has a feature that odor is reduced.

Using the dehydrated cake sample No., a water-regulating material was adjusted in accordance with (Example 4) to control the water content of pig manure, and a 14-day fermentation test was performed. The moisture control material used had a water content of 55%, and was mixed with pig manure, adjusted to a total water content of 65%, and subjected to fermentation at room temperature. High temperature fermentation started 3 days after the accumulation,
A maximum fermentation temperature of 71 ° C. was obtained. Odor generation was low and the water content was reduced to 45%.

(Example 7) Fermentation of vegetable waste In addition to livestock manure, vegetable waste treatment in the agricultural field is also an important issue. For the purpose of treating surplus crops or appropriate treatment of disease-damaged crops, the water content of the dehydrated cake is 45% to 80%.
Is adjusted in the range of B. Coagulans, B .; Cir
culans cells are inoculated to obtain a moisture control material. The water-regulated material has a high water content, but during the freshness maintaining period, the water is included and is released at the fermentation stage.

Therefore, the moisture control material appropriately absorbs the released moisture and maintains aerobic fermentation. It is reasonable to perform appropriate heat treatment at the fermentation treatment stage,
In this case, it is particularly necessary to add the moisture regulating material according to the invention, since the moisture is released rapidly. This is because, like vegetable waste, it initially contains moisture, but there is fish processing residue that releases a large amount of moisture when fermentation starts.

Using the dehydrated cake sample No., the moisture control material was adjusted in accordance with (Example 4), the water content of vegetable waste was adjusted, and a heat fermentation test for 6 hours was performed. The moisture content of the moisture control material used is 85%. The same amount of the water-regulating material and vegetable waste was added, and fermentation was performed using a heating-type agitating mixer under the condition that the temperature of the material to be heated was in the range of 55 ° C to 65 ° C. The moisture after 6 hours was 30% to 45%, depending on the type of vegetables. The finished fermentation product was obtained in granular form.

Example 8 Fermentation Treatment of Fish Treatment Residue When the fish treatment residue is left as it is, fermentation starts rapidly and liquefies. Therefore, it is preferable to perform a heating and deodorizing treatment by adding a moisture control material at about the same time as the generation of the residue. However, in practice, liquefaction proceeds before the heating treatment to generate an odor. The water content of the dehydrated cake is adjusted to the range of water that can be handled, that is, in the range of 45% to 80%, and the moisture control material for processing is adjusted according to (Example 4).

In the case of the treatment of fish processing residues, the liquefied matter generated contains fish blood and fish oil. However, when the moisture control material according to the present method is used, even if the moisture control material itself has a high water content, the dripping occurs. Can be improved. The characteristic of the cellulosic material is that it is both hydrophilic and lipophilic, and the moisture control material according to the present invention can be applied to a wide range of fermentation treatments.

Using the dehydrated cake sample No., the moisture control material was adjusted according to (Example 4), the water content of the fish treatment residue was adjusted, and the fermentation treatment was performed. The moisture content of the used moisture control material is 65%. While adding the same amount of water control material and fish processing residue, and stirring and mixing at room temperature for 12 hours,
Fermentation treatment was performed, and then heat fermentation treatment for 6 hours was performed. After a lapse of 12 hours of room temperature fermentation, the state becomes almost odorless, and no dripping has occurred. The heat fermentation treatment was performed according to (Example 8). The finished product has a water content of 30
% To 35%.

Example 9 Animal Fat Fermentation Treatment A large amount of animal fat is produced during the carcass demolition process and before display at a store. This tendency is due to recent trends in which the fat is not favored by consumers. In the case of this fat treatment,
The water content of the dewatered cake can be adjusted to 45% to 80% by using a dewatered cake, and the moisture control material can be adjusted and processed in the same manner as the treatment of fish processing residues. The fat that has released the oil and fat becomes a small sponge and has a shape susceptible to decomposition by soil bacteria.

Using the dehydrated cake sample No., a water-regulating material was adjusted in accordance with (Example 4) to control the water content of animal fat and fermented. The moisture content of the used moisture control material is 75% to 80%. The same amount of the water regulating material and animal fat was added, and a heat fermentation treatment for 6 hours was performed according to (Example 8). It is almost odorless,
The fats and oils are sufficiently absorbed and fermented, and there is almost no oily feeling. The final finished product has a moisture content of 30% to 35%.

(Embodiment 10) Coagulans,
B. Circulans cell growth conditions Table 3 shows the results of measuring the viable cell count of the sample by the Japan Food Research Laboratories, and growth at 55 ° C has been confirmed.

[0082]

[Table 3]

(Example 11) Many food-related residues such as processed food residues, household garbage, and cooked residues are generated, and the fermentation volume reduction process is attracting attention. As for the removal of salts contained in these residues, a rational method has not been developed, and there is a problem in using fermented products in the agricultural field. By using it, it is possible to economically reduce the volume. Since it is a familiar material to be treated, there are some places where combined use with heat sterilization is desired.

Using the dehydrated cake sample No., the moisture control material was adjusted according to (Example 4), the moisture control of the scrap noodles was performed, and a heat fermentation test for 6 hours was performed. The moisture content of the moisture control material used is 70%. The same amount of the moisture-regulating material and the scrap noodles are added, and a fermentation treatment is performed for 2 hours in a heating-type stirring and mixing machine under the condition that the temperature of the material to be heated is in a range of 55 to 65 ° C. The drying process was performed at a temperature of 145 ° C. The finished product was obtained in flake form.

[0085]

The present invention is embodied in the form described above, and has the following effects. According to the invention described in claim 1, it is fine and has many pores,
In addition, because natural cellulose having damaged micropores is used as a bacterial carrier, soil bacteria can be supported at a high concentration, and decomposition by the soil bacteria can be performed relatively smoothly. Easy to reduce volume and reduce fertilizer work amount. Therefore, the utilization of the finished fermentation product using the moisture control material according to the present invention can contribute to the stable supply of organic fertilized crops and horticultural plants. Further, the soil bacteria-supported moisture control material of the present invention has an advantage that it can be supplied inexpensively, in large quantities, and stably.

The present invention relates to a spore-forming bacterium, an aerobic bacterium, Coagulans, B .; The method utilizes Circulans cells, has durability against a high temperature region that is a cellulose decomposition environment, and is easy to handle because it is a spore-forming bacterium.

By using a pulp residue, which is a natural cellulosic material, as a carrier for the cells, not only the water content but also the fat and oil adsorption could be controlled.

In the present invention, the water content is 45 to 80% by weight.
, It can be handled in a lump such as flocculent, granular, lump, rod, clay, etc., and is easy to handle.

As described above, the soil-bacteria-supported water-regulating material of the present invention can be used as a water-regulating material by controlling natural cellulose having micropores, particularly pulp residue, by an economical method while sufficiently utilizing the characteristics of microorganisms. And could cope with a wide range of fermentation treatments.

According to the second aspect of the present invention, the storage stability can be ensured without requiring a prolonged drying treatment, and the fermentation treatment performance of the moisture control material is equal to that of the non-heated moisture control material. It could have fermentation treatment performance. Therefore, appropriate fermentation treatment can be performed on a wide range of materials to be treated in a short time, and at the same time, movement to a wide area or storage in a large amount can be performed. Further, as compared with the case where the final water content was set to 15% or less, the water absorption speed was excellent.

According to the third aspect of the present invention, it is possible to ensure sufficient storage stability of the moisture control material and to enable fermentation treatment in a wider area. In particular, storage for more than one week was made possible. It should be noted that almost the same results were obtained as compared with the fermentation treatment using the moisture control material according to the present invention in which the heating / drying treatment was not performed.

According to the fourth aspect of the present invention, when inoculating cells into the dehydrated cake, it was possible to minimize the outflow, increase the cost of cells and prevent slime from being generated in the reused clear water piping. In addition, by inoculating the cells at the stage of producing the moisture control material, a homogeneous bacterial species could be constituted at the stage of the moisture control treatment. This enabled a quick transition to the fermentation stage.

According to the fifth aspect of the present invention, various germs in the moisture-regulated material, Coagulan
s, B.S. Many of the resident bacteria other than the Circulans cells could be killed, and at the same time, the preservability of the moisture control material could be improved by heat treatment.

According to the sixth aspect of the invention, the heating time can be shortened, and the excessive capital investment cost and repair cost can be reduced. Further, since the treatment was performed at a high temperature, the cellulose decomposition fermentation could be performed in a short time.

In the present invention, when the temperature of the material to be heated is 80.degree. Coagulans, B .; Circ
Inoculation with B. ulans cells ensures B. ulans cells. Co
Agulans, B.A. The Circulans cells could be carried. Therefore, the microorganism treatment performance equivalent to that of the non-heat-treated moisture control material could be exhibited.

In the present invention, the temperature of the material to be heated exceeds 80 ° C.
Heat treatment in a temperature range of 50 ° C. or less has disadvantages such as fuel cost and addition of inoculation of cells. However, by setting the final moisture of the moisture control material to 15% or less, excellent preservability and wide range Can be moved and stored.

According to the invention as set forth in claim 7, by rapidly heating the dewatered cake, the odor attached to the pulp residue, for example, the anaerobic fermentation odor after the activated sludge treatment can be reduced in a short time, The incineration process could be eliminated.

Further, for example, when the purpose of fermentation of the material to be fermented is long for the purpose of treating cow dung, the dehydrated cake is not inoculated with the cells,
The method of inoculating the cells after the completion of the heat treatment was able to simplify the working steps.

In the present invention, the material to be heated is also
Heat treatment in a temperature range of more than 80 ° C. and 250 ° C. or less has disadvantages such as fuel cost. However, by setting the final moisture of the moisture control material to 15% or less, the storage stability is excellent, and the water content is excellent. Can be moved and stored.

[Brief description of the drawings]

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a diagram showing the evaluation results of cow manure fermentation treatment when the mixing ratio of the moisture control material of the present invention and cow manure is changed.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C12R 1:09) B09B 3/00 ZABA (72) Inventor Yoshihisa Someya 961 shares of Tsurugamine Honcho, Asahi-ku, Yokohama-shi, Kanagawa Prefecture. Company Someya (72) Inventor Isao Tomioka 3-11-11 Nihonbashi Honcho, Chuo-ku, Tokyo Co., Ltd. (72) Inventor Shinji Nakagawa 3-5-1 Nishi Zao, Nagaoka City, Niigata Prefecture Hokuetsu Paper Stock 4B033 NA12 NB02 NB12 NB45 NC04 ND04 ND20 4D004 AA02 AA12 BA10 CA18 CC07

Claims (7)

[Claims] 1. A dehydrated cake containing 45-80% by weight of water containing natural cellulose having micropores, and containing Bacillus Coagulans and / or Bacillus Bacillus.
s Circulans) (Science and Technology Research Institute of Biotechnology, FERM P-17807). 2. A soil-bacteria-supported moisture-regulating material, wherein the soil-bacteria is carried on a dehydrated cake having a final moisture content of more than 15% by weight and less than 45% by weight containing natural cellulose having micropores. 3. A soil-bacteria-supported moisture control material, wherein the soil-bacteria is carried on a dehydrated cake containing natural cellulose having micropores and having a final moisture content of 3 to 15% by weight. 4. A dewatered cake in which a fine pulp residue or sludge discharged from a papermaking and pulp mill is adjusted to a water content of 45 to 80% by weight, and the dewatered cake is subjected to Bacillus Coagulans and / or Bacillus thirakulance ( Bacillus Ci
rculans) (Faculty of Biotechnology, Institute of Industrial Science and Technology FE
RM P-17807), which comprises inoculating a soil bacterium and carrying the soil bacterium. 5. The method for producing a moisture control material for soil bacteria according to claim 4, further comprising a heat treatment at 55 to 80 ° C. 6. The method for producing a soil-bacteria-carrying moisture-regulating material according to claim 4, further comprising a heat treatment at a temperature higher than 80 ° C. and 250 ° C. or lower, cooling to 80 ° C. or lower, and inoculating the soil fungus. A method for producing a soil-bacteria-supported moisture regulating material, wherein the soil-bacteria is carried. 7. The dehydrated cake according to claim 4, which is heat-treated at 55 to 250 ° C., cooled to 80 ° C. or less, inoculated with the soil bacteria, and carried the soil bacteria. A method for producing a soil bacteria-carrying moisture control material.