JP2002053478A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a platelet agglutination inhibitor, an anti-inflammatory agent, an active oxygen scavenger, a radical scavenger, an antioxidant and a skin care preparation by selecting the substances having the platelet agglutination inhibition, the anti-inflammatory action, the active oxygen-scavenging action, the radical-scavenging action and the antioxidant action from the natural products and using them. SOLUTION: The extract from a plant, Canarium, is formulated to a platelet agglutination inhibitor, an anti-inflammatory agent, an active oxygen scavenger, a radical scavenger an antioxidant and a skin care preparation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a platelet aggregation inhibitor, an anti-inflammatory agent, an active oxygen scavenger, a radical scavenger, an antioxidant, and an extract from a plant, which contain an extract from a plant as an active ingredient. The present invention relates to an external preparation for skin contained.

[0002]

2. Description of the Related Art The causes and mechanisms of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, and various other skin diseases associated with rough skin are various, and one of the causes is one of the causes. One of them is due to platelet aggregation.

[0003] When platelets aggregate and activate,
In addition to physiologically causing hemostasis and pathologically causing thrombus formation, platelet aggregation is considered to be involved in the progression of arteriosclerosis, cancer metastasis, inflammation and the like. For this reason, attempts have been made to address the above-mentioned diseases by using substances that inhibit or suppress platelet aggregation, and aspirin, ticlopidine, sulfipyrazone, and the like have been used as platelet aggregation inhibitors. Product and side effects were a problem.

[0004] In recent years, active oxygen has been attracting attention as a factor particularly oxidizing biological components, and its adverse effect on living organisms has become a problem. Active oxygen is generated in the process of energy metabolism in living cells, and includes superoxide (ie, a superoxide anion generated by one-electron reduction of oxygen molecules) (.O ₂ ⁻ ), hydrogen peroxide (H
₂ O ₂ ) and hydroxy radical (.OH). These reactive oxygen species are essential for the bactericidal mechanism of phagocytic cells and play an important role in removing viruses and cancer cells.However, excessive production of reactive oxygen species attacks biological molecules that constitute membranes and tissues in vivo. Induce various diseases. For example, active oxygen is considered to degrade, denature, or crosslink biological tissues such as collagen, and oxidize fats and oils to produce lipid peroxide that damages cells. Is considered to cause aging such as wrinkling of the skin and decreased elasticity of the skin.

[0005] In a living body, the radical generated first based on oxygen is a superoxide, and other radicals such as a hydroxyl radical are generated via the superoxide. These radicals are the source of producing lipid peroxides involved in inflammation and aging. In particular, the hydroxyl radical is the most active of the active oxygen,
Immediately reacts with lipids, proteins, nucleic acids, carbohydrates and the like existing in the living body, causing peroxidation of lipids in cell membranes.
For this reason, ascorbic acid, spinach and the like are used as radical scavengers in order to suppress the production of lipid peroxide due to these in vivo radicals.

[0006] Superoxide dismutase (hereinafter referred to as "S
OD ”. ) Is a catalytic enzyme that converts superoxide, which is produced in cells and is initially generated based on oxygen, into hydrogen peroxide. The amount of SOD decreases with aging, and S
The decrease in OD increases the intracellular concentration of superoxide, reduces the activity of catalase and the like, which is a detoxifying enzyme for active oxygen, and causes superoxide to damage living bodies. For this reason, SOD itself, tocopherols, extracts of pentagon, and the like are used as SOD-like agents effective to compensate for the decrease in the amount of SOD.

[0007]

SUMMARY OF THE INVENTION An object of the present invention is, first, to find a substance having a platelet aggregation inhibitory action from natural products and to provide a platelet aggregation inhibitor containing the same as an active ingredient. . Another object of the present invention is to find a substance having an anti-inflammatory action from natural products, and to provide an anti-inflammatory agent containing the same as an active ingredient. Further, the third object of the present invention is to find a substance having an active oxygen scavenging action from natural products, and to provide an active oxygen scavenger containing the active ingredient as an active ingredient. Furthermore, the present invention fourthly aims to find a substance having a radical scavenging action from natural products, and to provide a radical scavenger containing the same as an active ingredient. Fifth, the present invention aims to find a substance having an active oxygen scavenging action and / or a radical scavenging action from natural products, and to provide an antioxidant containing the active ingredient as an active ingredient. further,
Sixth, the present invention provides an anti-inflammatory effect and / or a natural product.
Another object of the present invention is to find a substance having an antioxidant action and provide a skin external preparation containing the same.

[0008]

Means for Solving the Problems To achieve the above object, the present invention provides a platelet aggregation inhibitor, an anti-inflammatory agent, an active oxygen scavenger comprising an extract from a plant belonging to the genus Canalium as an active ingredient. Provided are a radical scavenger and an antioxidant, and a skin external preparation containing an extract from a plant belonging to the genus Canalium. In a preferred embodiment of the platelet aggregation inhibitor, anti-inflammatory agent, active oxygen scavenger, radical scavenger, antioxidant and skin external preparation of the present invention, the plant belonging to the genus Canalium is Uranium (Canari).
um pimela Koenig).

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.

The platelet aggregation inhibitor, anti-inflammatory agent, active oxygen scavenger, radical scavenger and antioxidant of the present invention are characterized by containing an extract from a plant belonging to the genus Canalium as an active ingredient.

In the present invention, the term “extract” includes an extract obtained from an extraction raw material by an extraction treatment, a diluent or a concentrate of the extract, a dried product obtained by drying the extract, Both of the crude product and the purified product.

In the present invention, a plant belonging to the genus Canalium is used as an extraction raw material, and preferably, a leaf of a plant belonging to the genus Canalium is used. Here, "leaves"
In general, it is composed of leaf blade, petiole, etc., but in the present invention, the whole leaf may be used as an extraction raw material,
A part of a leaf (for example, leaf blade) may be used.

The plant belonging to the genus Canarium is an evergreen tree of the family Orchidaceae. Plants belonging to the genus Canalium are widely distributed from Southeast Asia to the Pacific region, and can be obtained from these regions. The general forms of plants belonging to the genus Canalium are as follows. The leaves are pinnate compound and alternate. The flowers are small, dioecious, axillary or apical panicles. The sepals fuse to form a cylinder, and there are usually three petals.

[0014] Plants belonging to the genus Canalium include canlanium (Canarium album (Lour.) Raeusch), manila elemi (Canarium luzonicum (Bl.) A. Gray), canary tree (Canarium vulgare Leeth.), And wulan (crown) (C).
anarium pimela Koenig).

Canlanium (Canarium album (Lour.) Raeus
ch) is distributed from Indochina to southern China and is available from these regions. The general form of perilla is as follows. Leaves alternate, length 40
Odd pinnate compound leaf of ５０50 cm with 3-8 pairs of leaflets.
The leaflets are 6-14 cm long and 2-5 cm wide, oval, and full-edge. The flowers are pale yellowish white, small flowers of several mm in diameter, and bloom in panicles or racemes from the axils.

[0016] Manila Elemi (Canalium luzonicum (Bl.)
A. Gray) is native to the Philippines. The general form of Manila Elemi is as follows. There is a developed root at the base of the trunk. The leaves are leathery and odd-numbered pinnate compound leaves. The leaflets are 3-5 pairs, and there is a pair of pseudostipules at the base of the petiole,
It is precocious. The panicles emerge from the axils and have many small flowers.

[0017] Canary tree (Canarium vulgare Leet)
h.) is distributed from the Lesser Sunda Islands to the Moluccas and is available from these areas. The general form of canary tree is as follows. The bark is gray-brown and forms giant roots. The leaves are odd-numbered pinnate compound leaves having 3 to 5 pairs of leaflets, and the petiole has a vertical stripe. The inflorescence grows,
The flowers are yellow.

Uranium (Canarium pimela Koenig) is distributed throughout southern China and is available from these areas. The general form of Ulan is as follows. The bark is grayish white. Odd pinnate compound leaf, length 30
６０60 cm. The leaflets are 15 to 21 leaves, the leaf blades are oval or oval in shape, 5 to 15 cm in length and 3 in width.
5-7 cm. Flowers are white, panicles apical or axillary.

In the present invention, any of the above-mentioned plants belonging to the genus Canalium may be used as the extraction raw material, and two or more different plants may be used in combination. pimela Koen
ig) is preferably used.

In the present invention, it is preferable to use, as the extraction raw material, those that have been previously finely cut, crushed or crushed. In addition, as an extraction raw material, a material which has been dried in advance by the sun, a dryer or the like may be used.

In the present invention, it is preferable to use a polar solvent as the extraction solvent. Components that exhibit platelet aggregation inhibitory action, anti-inflammatory action, active oxygen scavenging action, radical scavenging action or antioxidant action contained in plants belonging to the genus Canalium have not yet been identified, but the components are extracted using a polar solvent. Can easily be extracted from plants belonging to the genus Canalium.

Specific examples of suitable polar solvents include water and hydrophilic organic solvents, and these can be used alone or in combination of two or more.

The water that can be used as the extraction solvent in the present invention includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. It is. Examples of the treatment applied to water include heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent which can be used as the extraction solvent in the present invention include lower aliphatic alcohols, hydrated lower aliphatic alcohols, acetone, chloroform, ethyl acetate and the like. Specific examples of lower aliphatic alcohols include methanol, ethanol, propanol,
Examples thereof include 1,3-butylene glycol, glycerin, propylene glycol, and isoprene glycol.

In the present invention, when a mixed solution of two or more polar solvents is used as an extraction solvent, the mixing ratio can be appropriately adjusted. For example, when a mixed solution of water and a lower aliphatic alcohol is used, the mixing ratio of water and the lower aliphatic alcohol can be 7: 3 to 2: 8 (weight ratio).

The extraction treatment in the present invention is not particularly limited as long as the soluble components contained in the plant belonging to the genus Canalium can be eluted into the extraction solvent, and can be performed according to a conventional method. In the extraction treatment, it is not necessary to employ a special extraction method, and any device can be used at room temperature or under reflux.

For example, an extraction raw material is put into a treatment tank filled with an extraction solvent, and a soluble component can be eluted with occasional stirring. At this time, the amount of the extraction solvent is usually 5 to 15 times (weight ratio) of the extraction raw material, the extraction time is usually 1 to 3 hours, and the extraction temperature is usually from room temperature to 95%.
° C.

After the soluble component is eluted by the extraction treatment, the extract is subjected to treatment such as filtration and centrifugation to remove the extraction residue, thereby obtaining an extract. The obtained extract is a diluted or concentrated solution of the extract, a dried product of the extract, or a crude product or a purified product thereof. Processing may be performed.

The treatment such as purification can be specifically carried out by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, etc., and these treatments are carried out by the biological activity of extracts from plants belonging to the genus Canalium. It is performed within a range that does not cause a decrease in platelet aggregation inhibitory activity, anti-inflammatory activity, active oxygen scavenging activity, radical scavenging activity or antioxidant activity.

The extract from the plant belonging to the genus Canalium obtained as described above has a platelet aggregation inhibitory action, an anti-inflammatory action, an active oxygen scavenging action, a radical scavenging action, or an antioxidant action. The mechanism of action of these actions has not been elucidated so far, but it is clear from the examples described below that extracts from plants belonging to the genus Canalium have these actions.

The extract from the plant belonging to the genus Canalium can be used as it is as a platelet aggregation inhibitor, an anti-inflammatory agent, an active oxygen scavenger, a radical scavenger or an antioxidant. Can also be used. In the case of formulation, a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other optional auxiliaries can be added to facilitate storage and handling. An extract from a plant belonging to the genus Canalium can be made into any dosage form such as a powder or a tablet by formulation.

The platelet aggregation inhibitor of the present invention can inhibit platelet aggregation. Aggregation and activation of platelets in vivo causes thrombus formation, development of arteriosclerosis, cancer metastasis, inflammation, and the like. Therefore, according to the platelet aggregation inhibitor of the present invention, diseases such as inflammation associated with platelet aggregation can be improved by suppressing platelet aggregation.

The anti-inflammatory agent of the present invention can prevent or treat inflammation. Since the extract from a plant belonging to the genus Canalium, which is an active ingredient of the anti-inflammatory agent of the present invention, also has a platelet aggregation inhibitory effect, according to the anti-inflammatory agent of the present invention, platelet aggregation is involved through the platelet aggregation inhibitory effect. Can be prevented or treated. However, the inflammation that can be prevented or treated by the anti-inflammatory agent of the present invention is not limited to inflammation involving platelet aggregation. When the purpose is to prevent or treat inflammation involving platelet aggregation, the active ingredient of the anti-inflammatory agent of the present invention can be used as the platelet aggregation inhibitor of the present invention.

The active oxygen scavenger of the present invention can scavenge active oxygen. Here, “active oxygen” includes superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like. The active oxygen elimination agent of the present invention can be suitably used for erasing superoxide particularly among these active oxygen species. Excessive production of active oxygen attacks biological molecules constituting membranes and tissues in the living body and induces various diseases.Accordingly, the active oxygen scavenger of the present invention improves the above-mentioned diseases involving active oxygen. can do. For example, active oxygen is considered to degrade, denature, or crosslink biological tissues such as collagen, and oxidize fats and oils to produce lipid peroxide that damages cells. Is considered to cause aging such as wrinkling of the skin and decreased elasticity of the skin. Therefore, according to the active oxygen scavenger of the present invention, it is possible to prevent the formation of wrinkles in the skin and a decrease in the elasticity of the skin by suppressing the production of lipid peroxide.

The radical scavenger of the present invention can scavenge radicals. Here, the “radical” means a molecule or an atom having one or more unpaired electrons. The radical that can be eliminated by the radical scavenger of the present invention is not particularly limited, but the radical scavenger of the present invention can be suitably used to scavenge radicals such as superoxide, hydroxy radical, and DPPH.
In vivo radicals such as superoxide and hydroxyl radicals are a source of producing lipid peroxides involved in inflammation and aging.In particular, hydroxy radicals are immediately linked to lipids, proteins, nucleic acids or carbohydrates and the like present in the body. Reacts chemically, causing peroxidation of cell membrane lipids. Therefore, according to the radical scavenger of the present invention, inflammation and aging can be prevented by suppressing the production of lipid peroxide by these in vivo radicals.

The antioxidant of the present invention can prevent oxidation of a substance. Since the extract from the plant belonging to the genus Canalium, which is an active ingredient of the antioxidant of the present invention, has both an active oxygen scavenging action and a radical scavenging action, according to the antioxidant of the present invention, active oxygen and radicals are involved. Oxidation can be effectively prevented. However, the oxidation that can be prevented by the antioxidant of the present invention is not limited to oxidation involving active oxygen and radicals. The substance that the antioxidant of the present invention can prevent oxidation is not particularly limited,
The antioxidant of the present invention can be suitably used particularly for preventing oxidation of biological components such as oils and fats. When the purpose is to prevent oxidation involving active oxygen and radicals, the active ingredient of the antioxidant of the present invention should be the active oxygen scavenger of the present invention and / or the radical scavenger of the present invention. Can be.

The external preparation for skin of the present invention is characterized by containing an extract from a plant belonging to the genus Canalium. Here, “skin external preparation” means various drugs applied to the skin, and includes, for example, cosmetics, quasi-drugs, and pharmaceuticals. Specific examples of skin external preparations include, for skin, ointments, cataplasms, creams, emulsions, lotions,
Packs, jellies and the like can be exemplified, and as to the scalp, tonics, rinses, shampoos, astringents and the like can be exemplified.

An extract from a plant belonging to the genus Canalium can exert a platelet aggregation inhibitory action, an anti-inflammatory action, an active oxygen scavenging action, a radical scavenging action, and an antioxidant action. These effects can be imparted to the external preparation for skin by containing the extract from E. coli. The external preparation for skin of the present invention may be an extract from a plant belonging to the genus Canalium, which may be a compound that is blended with any main agent or auxiliary agent that does not interfere with its physiological activity, or may be obtained from a plant belonging to the genus Canalium. The extract may be one containing an extract as a main component.

When an external preparation for skin is prepared by blending an extract from a plant belonging to the genus Canalium, an optional auxiliary agent is added to convert the extract from the plant belonging to the genus Canalium into an arbitrary dosage form. It can be formulated.

The amount of the extract from the plant belonging to the genus Canalium in the external preparation for skin of the present invention depends on the strength of the activity of the extract from the plant belonging to the genus Canalium and the amount of the extract from the plant belonging to the genus Canalium. Can be appropriately adjusted depending on the type of skin external preparation,
% By weight.

In the external preparation for skin of the present invention, the following can be exemplified as those which can be used in combination with an extract from a plant belonging to the genus Canalium. When the following constituents are used in combination with the extract from the plant belonging to the genus Canalium, a synergistic effect between the extract and the constituent used in combination with the plant from the genus Canalium is more than expected. May have an excellent use effect.

Astringents: citric acid or a salt thereof, tartaric acid or a salt thereof, lactic acid or a salt thereof, aluminum chloride, aluminum potassium potassium sulfate, allantochlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenol sulfonate, zinc sulfate, zinc extract, Age extract, Hamamelis extract,
Gennoshoko extract, chacatechins, gypsophila extract, lycopodium extract, hypericum extract, rhubarb extract, cornflower extract, horsetail extract, kizuta extract, cucumber extract, marronnier extract, salvia extract, melissa extract and the like.

Bactericidal and antibacterial agents: benzoic acid, sodium benzoate, paraoxybenzoate, distearylmethylammonium chloride, benzethonium chloride, alkyldiaminoethylglycine chloride solution, chlorhexidine hydrochloride,
Orthophenyl phenol, Photosensitizer No. 101, Photosensitizer 2
No. 01, salicylic acid, sodium salicylate, sorbic acid, halocarban, resorcinol, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan hydrolyzate, diu extract, kudin extract, enmeiso extract, loquat extract, awaurushi extract, hop extract, Yucca extract,
Aloe extract, Cahi extract, gajutsu extract, etc.

Whitening agent: ascorbic acid and its derivatives, sulfur, placental extract, kojic acid and its derivatives,
Glucosamine and its derivatives, Azelain and its derivatives, Arbutin and its derivatives, Hydroxycinnamic acid and its derivatives, Glutathione, Arnica extract, Ogon extract, Senkyu extract, Sohakuhi extract, Psychoextract, Bohu extract, Hamabofu extract, Mannentake mycelium culture Or its extract, Gymnema extract, linden extract, peach leaf extract, Eights extract, kujin extract, Jiyu extract, Touki extract,
Yokuinin extract, oyster leaf extract, rhubarb extract, botanical extract, hammerellis extract, marronie extract, hypericum extract, hydrangea extract, oil-soluble licorice extract (liquorice hydrophobic flavone, glabridine, glabrene, lycochalcone A) and the like.

UV absorbers: β-isopropylfuranone derivative, urocanic acid, ethyl urocanate, oxybenzone, oxybenzonesulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, sinoxate, methyl diisopropylcinnamate, octyl methoxycinnamate, paraamino Glyceryl benzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, butylmethoxydibenzoylmethane, titanium oxide, β-carotene, γ-oryzanol, rice bran extract, aloe extract, birch extract , Birch extract, chamomile extract, black beetle extract, hawthorn extract, henna extract, butterfly Extract, horse chestnut extract, ginkgo leaf extract, chamomile extract, hawthorn extract, oil-soluble licorice extract, and the like.

Moisturizers: serine, glycine, threonine,
Alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin heparin, glycerophospholipid, glyceroglycolipid, sphingolipid, glycosphingolipid, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof , Pectin, alge colloid, bifidobacterium fermented product, lactic acid fermented product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol , Below oil extract, yokuinin extract, jasmine extract, diatom extract, diatom extract, kidachi aloe extract, burdock extract, maroni extract, mannen wax extract, arnica extract, wheat bran, rice bran extract .

Cell activator: riboflavin or its derivative, pyridoxine or its derivative, nicotinic acid or its derivative, pantothenic acid or its derivative, α-tocopherol or its derivative, arnica extract, carrot extract, rape ginseng extract, eleuthero extract,
Loofah extract (saponin), radish extract, white birch extract, psyllium extract, peanut extract, peony extract, mukuroji extract, safflower extract, ashitaba extract, loquat leaf extract, hinoki sorghum extract, saxifactinia extract, yellow mulberry extract, salvia extract, garlic extract,
Mannen wax extract and the like.

Anti-inflammatory and anti-allergic agents: azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or its derivatives, glycyrrhetinic acid or its derivatives, photosensitive element 301, photosensitive element 401
No., diphenhydramine hydrochloride, tranexamic acid or a derivative thereof, adenosine phosphate, estradiol, ethrone, ethinyl estradiol, cortisone, hydrocortisone, prednisone, progesterone, corticosterone, arnica extract, intinko extract, sanshishi extract, juyaku extract, horse chestnut extract , Liquorice extract, syrup extract, mugwort extract, sage extract, gentian extract, psycho extract, senkyu extract, boufu extract, Achillea millefolium extract, spinach extract, perilla extract, etc.

Antioxidant / active oxygen scavengers: dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, melissa extract, ougon extract, age extract, loquat leaf extract, Hop extract, Hamamelis extract,
Peony extract, sage extract, kina extract, chamomile extract, eucalyptus extract, perilla extract, ginkgo leaf extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, mace extract, laurel extract, clove extract, turmeric extract, willow extract, etc.

In the case of producing cosmetics containing an extract from a plant belonging to the genus Canalium, selection of other raw materials for producing cosmetics is hardly limited, and general cosmetics as exemplified below are used. Both substrates and auxiliaries can be used.

Oils and fats: soybean oil, linseed oil, drill oil, sesame oil, bran oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil,
Peanut oil, cocoa oil, mokuro, palm oil, palm kernel oil,
Tallow, mink oil, egg yolk oil, jojoba oil, evening primrose oil, horse oil.

Waxes: carnauba wax, candelilla wax, beeswax, salami beeswax, whale wax, Celax, lanolins.

Hydrocarbons: liquid paraffin, vaseline,
Microcrystalline wax, ceresin, squalane, polyethylene powder.

Fatty acids: stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hevenic acid,
Lanolinic acid, oleic acid, undecylenic acid, isostearic acid.

Alcohols: lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol,
Hexadecyl alcohol, 2-octyldodecanol,
Glycerin, sorbitol, propylene glycol,
1,3-butylene glycol, ethylene glycol and polymers thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol.

Esters: decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, propylene glycol dioleate, diethyl phthalate, Propylene glycol monostearate, ethylene glycol monostearate, glyceryl monostearate, glyceryl trimmyristate, lanolin acetate, cetyl lactate.

Surfactants: anionic surfactants, cationic surfactants, amphoteric surfactants, and nonionic surfactants.

Fragrance: menthol, carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil.

The platelet aggregation inhibitor, anti-inflammatory agent, active oxygen scavenger, radical scavenger, antioxidant and external preparation for skin of the present invention described above are preferably applied to humans. The present invention may be applied to animals other than humans as long as the effects of the present invention are exerted.

[0060]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to these examples.

[Production Example 1] Uranium (Canarium pimela)
Koenig) leaves were crushed, and the obtained crushed material was used as an extraction raw material. To 100 g of the extraction raw material, water as an extraction solvent,
50% ethanol (weight ratio of water to ethanol 1: 1)
Then, 1000 ml each of ethanol and ethanol were added, and the mixture was gently stirred for 2 hours while keeping the temperature at 80 ° C., and then filtered using a filter paper to obtain an extract from uranium leaves. Extract 4
After concentration under reduced pressure at 0 ° C., the residue was dried with a reduced pressure drier to obtain an extract and dried product. The yields (% by weight) of the extracts obtained using various extraction solvents were as shown in Table 1 below.

[Table 1] Sample No. Extraction Solvent extraction yield (wt%) 1 Water 21.5 2 50% ethanol 27.0 3 Ethanol 22.6

[Formulation Example 1] A lotion having the following composition was produced by a conventional method using the 50% ethanol extract from the leaves of uranium obtained in Production Example 1. Uranium extract 5.0% by weight Glycerin 3.0% by weight 1,3-butylene glycol 5.0% by weight Ethanol 3.0% by weight Polyoxyethylene olein alcohol 0.5% by weight Methyl paraben 0.1% by weight Citric acid 0 0.1% by weight Sodium citrate 0.1% by weight Hyaluronic acid 0.2% by weight Perfume 0.05% by weight Purified water balance

[Formulation Example 2] Using the ethanol extract obtained from the leaves of uranium obtained in Production Example 1, an emulsion having the following composition was produced by a conventional method. Uranium extract 1.0% by weight Dipotassium glycyrrhizinate 0.5% by weight Chamomile extract 0.5% by weight Hawthorn extract 0.5% by weight Warmoko extract 0.5% by weight Hyaluronic acid 0.03% by weight Juyaku extract 0.3 wt% Arnica extract 0.3 wt% Stearic acid 2.0 wt% Cetyl alcohol 1.5 wt% 1,3-butylene glycol 3.0 wt% Glycerin 2.0 wt% Vaseline 5.0 wt% Liquid paraffin 10.0% by weight Polyoxyethylene (10EO) oleate 2.0% by weight Polyethylene glycol 1500 3.0% by weight Triethanolamine 1.0% by weight Ethyl paraben 0.3% by weight Perfume Appropriate amount Purified water Remainder

[Formulation Example 3] A pack having the following composition was produced by a conventional method using the water extract from the leaves of uranium obtained in Production Example 1. Uranium extract 1.0% by weight Allantoin 0.1% by weight Glutathione 0.1% by weight Birch extract 0.5% by weight Saw amber extract 0.5% by weight Stearyl glycyrrhetinate 0.5% by weight Hydrolyzed conchiolin 0 0.2% by weight Urea 3.0% by weight Hamamelis extract 0.3% by weight Polyvinyl alcohol 13.0% by weight Ethyl alcohol 7.0% by weight Dipropylene glycol 5.0% by weight Polyoxyethylene (60EO) hydrogenated castor oil 5 0.0% by weight Olive oil 5.0% by weight Tocopherol acetate 0.2% by weight Phenoxyethanol 0.5% by weight Perfume Appropriate amount Purified water balance

Test Example 1 The water extract (sample 1), 50% ethanol extract (sample 2) and ethanol extract (sample 3) obtained in Production Example 1 were tested for their platelet aggregation inhibitory activity by the following method. Was tested.

(1) Blood of Japanese white rabbits was 77 mM-
One tenth of EDTA was added, and the precipitate was removed by centrifugation at 1000 rpm for 10 minutes. Centrifuge supernatant for 2100
After centrifuging at 10 rpm for 10 minutes to collect platelets,
Platelet wash (0.15 M sodium chloride solution, 0.1
15 M Tris-HCl buffer (pH 7.4), 77 mM
Washing was performed twice using an EDTA (sodium ethylenediaminetetraacetate) solution (pH 7.4) mixed at a ratio of 90: 8: 2. A platelet suspension (145 mM sodium chloride solution, 5 mM potassium chloride, 5.5 mM glucose-containing 10 mM HEPES buffer (pH 5.5) was added to the collected platelets so that the platelet count was 300,000 / μl.
7.4)). 1 μl of a calcium chloride solution was added to 233 μl of the platelet suspension thus prepared, and the mixture was incubated at 37 ° C. for 1 minute. 1 μl of a sample solution containing each of samples 1 to 3 was added thereto, and the mixture was further kept at 37 ° C. for 2 minutes and then stirred for 1 minute. Next, 25 μl of a collagen solution was added, and the mixture was kept at 37 ° C. for 10 minutes, and then the visible light transmittance was measured, and this was used as an index of the platelet aggregation state. The thus measured visible light transmittance is hereinafter referred to as “visible light transmittance A”.

(2) The visible light transmittance was measured in the same manner as in the above (1) except that the sample solution was not added. In addition,
The visible light transmittance thus measured is hereinafter referred to as “visible light transmittance B”.

(3) The platelet aggregation inhibition rate (%) of Samples 1 to 3 was determined based on the following equation.

[0070]

Formula 1: Platelet aggregation inhibition rate (%) = [(BA) / A] × 100

In the formula, “A” represents the visible light transmittance A
And “B” represents visible light transmittance B.

(4) The visible light transmittance was measured in the same manner as described above while changing the sample concentration stepwise, and the sample concentration at which the platelet aggregation inhibition rate became 50% was determined by interpolation. Sample 1
3 inhibiting platelet aggregation (50% inhibitory concentration (μg / m
l)) was as shown in Table 2 below.

[Table 2] Sample No. 50% inhibitory concentration (μg / ml) 1 126.9 2 91.1 3 89.5

As shown in Table 2, the water extract from uranium leaves (sample 1) and the 50% ethanol extract (sample 2)
Each of the ethanol extract and the ethanol extract (sample 3) showed platelet aggregation inhibitory activity. Among these extracts, especially 50
% Ethanol extract and the ethanol extract showed excellent platelet aggregation inhibitory activity.

Test Example 2 The water extract (sample 1), 50% ethanol extract (sample 2), and ethanol extract (sample 3) obtained in Production Example 1 were tested for superoxide elimination by the following method. Was tested.

(1) 3 mM xanthine, 3 mM EDT
A, 1.5 mg / ml BSA solution, 0.75 mM nitro blue tetrazolium (NBT)
and 0.05 M Na ₂ CO ₃ buffer (pH 10.
2) Transfer 2.4 ml to a test tube, and add a sample solution in which samples 1 to 3 are dissolved in distilled water or DMSO, respectively.
1 ml was added and left at 25 ° C. for 10 minutes. Next, 0.1 ml of a xanthine oxidase solution was added, the mixture was rapidly stirred, and allowed to stand at 25 ° C. for 20 minutes. Thereafter, 0.1 ml of a 6 mM copper chloride solution was added to stop the reaction, and 560 n
The absorbance at m was measured. Hereinafter, this absorbance is referred to as “absorbance at the time of addition of the sample solution and the enzyme solution”.

(2) The absorbance was measured in the same manner as in (1) except that the xanthine oxidase solution was not added.
Hereinafter, this absorbance is referred to as “absorbance when the sample solution is added and the enzyme solution is not added”.

(3) Absorbance was measured in the same manner as in (1) except that distilled water was added instead of the sample solution. Hereinafter, this absorbance is referred to as “absorbance when no sample solution is added and when an enzyme solution is added”.

(4) Instead of adding distilled water instead of the sample solution and not adding the xanthine oxidase solution,
The absorbance was measured in the same manner as in the above (1). This absorbance is referred to as "absorbance when no sample solution is added and no enzyme solution is added".

(5) The superoxide erasure rates of Samples 1 to 3 were determined based on the following equation.

[0081]

[Equation 2] erasure rate (%) = _{[1- (S t -S o) /} (B t -
B _o )] × 100

In the formula, “ _St ” indicates the absorbance when the sample solution is added and the enzyme solution is added, “S _o ” indicates the absorbance when the sample solution is added and the enzyme solution is not added, and “B _t ” indicates the sample solution. Absorbance when no enzyme solution is added and when an enzyme solution is added, and “B _o ” indicates absorbance when no sample solution is added and no enzyme solution is added.

The absorbance was measured in the same manner as described above while changing the sample concentration stepwise.
Was determined by an interpolation method. Superoxide erasing activity (50% erasing concentration (μg / m
l)) was as shown in Table 3 below.

[Table 3] Sample No. 50% erase concentration (μg / ml) 1 10.6 2 5.4 3 6.8

As shown in Table 3, a water extract from uranium leaves (sample 1) and a 50% ethanol extract (sample 2)
And the ethanol extract (Sample 3) showed superoxide scavenging activity. Among these extracts, particularly, a 50% ethanol extract and an ethanol extract showed excellent superoxide scavenging activity.

Test Example 3 With respect to the water extract (sample 1), 50% ethanol extract (sample 2) and ethanol extract (sample 3) obtained in Production Example 1, DPPH, a very stable radical, was used. Was used to test the radical scavenging action by the following method.

(1) 1.5 × 10 ⁻⁴ M DPPH (1,
1-Diphenyl-2-picrylhydraz
yl) To 3 ml of the ethanol solution, 3 ml of the sample solution containing each of the samples 1 to 3 was added, the container was immediately sealed, shaken, and allowed to stand for 30 minutes. Then 520 nm
Was measured. Hereinafter, this absorbance is referred to as “absorbance at the time of addition of the sample solution”.

(2) Except that the solvent (water or DMSO) is added instead of the sample solution, the same procedure as in (1) above is repeated.
The absorbance at 20 nm was measured. Hereinafter, this absorbance is referred to as “the absorbance of the control test”.

(3) 3 ml of a sample solution containing each of samples 1 to 3 was added to 3 ml of ethanol, and immediately
The absorbance at 20 nm was measured. Hereinafter, this absorbance is referred to as “absorbance of blank test”.

(4) The radical scavenging rates of Samples 1 to 3 were determined based on the following equation.

[0091]

Formula 3: Radical scavenging rate (%) = [1- (B-C) /
A] × 100

In the formula, “A” represents the absorbance of the control test, “B” represents the absorbance when the sample solution is added, and “C” represents the absorbance of the blank test.

The absorbance was measured in the same manner as above while changing the sample concentration stepwise, and the sample concentration at which the radical scavenging rate was 50% was determined by interpolation. The radical scavenging activity (50% scavenging concentration (μg / ml)) of Samples 1 to 3 was as shown in Table 4 below.

[Table 4] Sample No. 50% erase concentration (μg / ml) 1 48.8 2 23.6 3 21.5

As shown in Table 4, a water extract (sample 1) and a 50% ethanol extract (sample 2) from the uranium leaves were used.
And the ethanol extract (Sample 3) showed a radical scavenging activity. Among these extracts, especially 50%
The ethanol extract and the ethanol extract showed excellent radical scavenging activity.

Test Example 4 A lotion-like coating solution a containing 50% ethanol extract obtained in Production Example 1 and a coating solution having the same composition as coating solution a except that it does not contain the 50% ethanol extract b (control) were prepared and tested for their ability to prevent razor defeat. In addition, razor defeat is a symptom in which after shaving, such as a beard, the skin turns red and irritates, or becomes swollen, feverish, or itchy. It is a symptom caused by small incisions from which bacteria infect and inflame.

The composition of each coating solution was as shown in Table 5 below.

[Table 5] Coating solution a Coating solution b Uranium extract 1.0 g None 1,3-butylene glycol 5.0 g 5.0 g Glycerin 1.0 g 1.0 g Ethanol 6.0 g 6.0 g Nonionic Surfactant 0.5 g 0.5 g Purified water Remaining Remaining (total amount of both coating liquid a and coating liquid b is 100 ml)

10 male subjects who lose a razor
Each group was divided into two groups, and one group was coated with the coating solution a, and the other group was coated with the coating solution b on the skin immediately after shaving, and the razor losing prevention effect was evaluated according to the following criteria.

If the razor loses, the result is “significantly effective”. If the razor loses weakly, the result is “effective”. If it is not recognized, it is judged as "invalid", and "A" if the number of subjects judged as "invalid" is less than 20%, "B" if it is 20% or more and less than 50%, 50% If it is more than 80%, "C",
When it was 80% or more, it was evaluated as "D".

As a result, the razor loss prevention effect of the coating solution a was evaluated as “A”, and the razor loss prevention effect of the coating solution b was evaluated as “D”. In addition, at the same time as the determination of the razor losing prevention effect, impressions on the degree of irritation to the skin (irritating feeling) were obtained, and all the subjects answered that neither of the application liquids felt the irritation. The results indicated that the extract from uranium leaves had a razor defeat prevention effect, ie, an anti-inflammatory effect.

Test Example 5 The lotion prepared in Formulation Example 1 was tested for the anti-aging effect and the beautiful skin effect by the following methods.

20 healthy women (25 to 45 years old) were used as test subjects. The test subjects were asked to use the lotion prepared in Formulation Example 1 for 2 months, and a questionnaire survey was conducted on the elasticity and smoothness of the skin after use. Then, the anti-aging effect and the beautiful skin effect were evaluated. At this time, a lotion containing no uranium extract was used as control example 1.

The evaluation criteria were "A" for valid cases, "B" for slightly valid cases, "C" for slightly valid cases, and "D" for invalid cases.

The evaluation of the anti-aging effect and the beautiful skin effect was as shown in Table 6 below.

[0106] [Table 6] examined body A B C D Formulation Example 1 lotion twelve six 2 0 people Reference Example 1 Lotion 0 people 2 people 8 people 10 people

As shown in Table 6, the lotion prepared in Formulation Example 1 was superior to the lotion of Comparative Example 1 in the anti-aging effect and the beautiful skin effect. from this result,
It was revealed that the extract from uranium leaves has an antiaging effect and a beautiful skin effect.

[0108]

According to the present invention, a platelet aggregation inhibitor, an anti-inflammatory agent, an active oxygen scavenger, a radical scavenger and an antioxidant are provided. Further, according to the present invention, there is provided a skin external preparation having a platelet aggregation inhibitory action, an anti-inflammatory action, a scavenging action of active oxygen, a radical scavenging action or an antioxidant action. ADVANTAGE OF THE INVENTION According to the platelet aggregation inhibitor of this invention, it can prevent or improve various skin diseases and rough skin related to platelet aggregation. Further, according to the anti-inflammatory agent of the present invention, inflammation particularly involving platelet aggregation among inflammations can be effectively prevented or ameliorated. Further, according to the active oxygen scavenger and the radical scavenger of the present invention, it is possible to effectively prevent or improve aging phenomena such as wrinkle formation and skin elasticity reduction involving active oxygen and in vivo radicals. Further, according to the antioxidant of the present invention, aging phenomena such as wrinkle formation and decrease in skin elasticity can be effectively prevented or improved through prevention of oxidation of biological components.

Claims (12)

[Claims] A platelet aggregation inhibitor comprising an extract from a plant belonging to the genus Canalium as an active ingredient. 2. The platelet aggregation inhibitor according to claim 1, wherein the plant belonging to the genus Canalium is Uranium (Canarium pimela Koenig). 3. An anti-inflammatory agent comprising an extract from a plant belonging to the genus Canalium as an active ingredient. 4. The anti-inflammatory agent according to claim 3, wherein the plant belonging to the genus Canalium is uranium (Canarium pimela Koenig). 5. An active oxygen scavenger comprising an extract from a plant belonging to the genus Canalium as an active ingredient. 6. The active oxygen scavenger according to claim 5, wherein the plant belonging to the genus Canalium is uranium (Canarium pimela Koenig). 7. A radical scavenger comprising an extract from a plant belonging to the genus Canalium as an active ingredient. 8. The radical scavenger according to claim 7, wherein the plant belonging to the genus Canalium is uranium (Canarium pimela Koenig). 9. An antioxidant comprising an extract from a plant belonging to the genus Canalium as an active ingredient. 10. The antioxidant according to claim 9, wherein the plant belonging to the genus Canalium is uranium (Canarium pimela Koenig). 11. An external preparation for skin, comprising an extract from a plant belonging to the genus Canalium. 12. The external preparation for skin according to claim 11, wherein the plant belonging to the genus Canalium is Uranium (Canarium pimela Koenig).