JP2000198714A - Antioxidant and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant having high anti-oxidizing power, controlling active oxygen and lipid peroxide formed in vivo, having excellent safety by making the antioxidant include an extract of Bursera lancifolia. SOLUTION: This antioxidant contains preferably 0.001-20.0 wt.%, calculated as dried substance based on the total of an extract, of Bursera lancifolia (gomosilla) (a plant of the genus Brucella of the family Burseraceae native to Mexico), preferably an extract from either of leaf or stem of Bursera lancifolia or a mixture of both of the leaf and the stem. Preferably, the amount of an extracting solvent such as water and a water-soluble solvent (e.g. ethanol) used is 100 pts.-wt. based on 5-50 pts.wt. of dried plant or green plant calculated as dried plant.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an antioxidant and a composition containing the antioxidant. Furthermore, the present invention relates to an external preparation for skin that can be used in the fields of pharmaceuticals, quasi-drugs, cosmetics and the like containing the antioxidant.

[0002]

2. Description of the Related Art In recent years, active oxygen generated in a living body has
It has become clear that it reacts with unsaturated fatty acids to produce lipid peroxide, which has a bad effect on the human body. For example, active oxygen ischemic injury and radiation injury, lipid peroxide and its oxidized degradation products act on nucleic acids and proteins, and cause arteriosclerosis, hypertension, vascular injury, hepatic dysfunction, and retinopathy caused by them. And cataracts. Especially on the skin,
Because it is directly stimulated by environmental factors such as ultraviolet rays, active oxygen is likely to be generated, increasing active oxygen concentration, abnormal pigmentation such as spots and freckles such as the production of lipid peroxide, inflammation, edema, necrosis, wrinkles, Aging and the effects are remarkable.

In addition, many cosmetics, pharmaceuticals, foods and beverages contain fats and oils, which react with active oxygen during storage or use to produce lipid peroxides, thereby reducing quality and reducing nutrition. -Expression of toxicity to the human body is a major problem.

[0004] For this reason, in order to improve the abnormalities of lipid peroxide in the living body, investigations for drugs having an antioxidant action have been widely conducted. As a typical example, fat-soluble tocopherol (vitamin E) is used as a natural product antioxidant.
And water-soluble ascorbic acid (vitamin C), and BHT (3,5-tert-butyl-4-hydroxyto) as a synthetic antioxidant.
len) and BHA (2, (3) -tert-butyl-hydroxyanysol) and the like, but their effects were not satisfactory.

On the other hand, many attempts have been made to obtain a substance having a high antioxidant activity in order to improve abnormalities of lipid peroxide in the living body, and various crude drug extracts have been disclosed (for example, Japanese Patent Application Laid-Open No. H11-157556). Kaihei 5-246877, JP-A-8-92053,
JP-A-8-301745).

[0006]

However, the above crude drug extract is more effective than tocopherol and ascorbic acid.
Although it has a somewhat high antioxidant effect, its effect is not satisfactory. Further, the synthetic antioxidants BHT and BHA have problems such as suspected carcinogenicity. Therefore, in addition to these antioxidants, a substance having similar means for suppressing lipid peroxide production and having high safety is desired.

[0007]

In view of such circumstances, the present inventors have conducted intensive studies to improve the problems of the prior art, and as a result, surprisingly, Brucella, a plant belonging to the orchid family. Ransifolia (gomosilla) [Bursera
lancifolia (GOMOSILLA)] extract has strong free radical scavenging and antioxidant effects.

[0008] That is, the present invention relates to a plant of the family Orchidaceae, Brucella ransifolia (Gomosilla) [Bursera
lancifolia (GOMOSILLA)], and an antioxidant characterized by comprising an extract of
It is intended to provide a composition for a food, a medicine and the like.

[0009]

Embodiments of the present invention will be described below in detail. Burser Lansifolia used in the invention (Burser
a lancifolia) is a type of Orchidaceae (Burs) native to Mexico.
eraceae) A plant belonging to the genus Brucella.

[0010] The extract of Bursera lancifolia used in the present invention is extracted from a part or the whole of a plant such as a leaf, a stem, a flower, a seed, a fruit, a bark, a rhizome, a root, etc. of the plant. It is obtained by doing. Preferably, it is obtained by extracting from one of the leaves or the stem, or a mixture of both. In general, dried or raw plants are used as they are or cut. Extraction solvent to be used is 5 to 5 per dry matter or dry matter of raw plants.
For 50 parts, 100 parts of the following extraction solvent is used.

[0011] The extraction solvent is generally water, lower 1
Polyhydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol, etc.), lower alkyl esters (ethyl acetate, etc.) ), Hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether), acetonitrile and the like. These solvents may be used alone or as a mixture of two or more. Preferably, water or a water-soluble solvent (a solvent that can be mixed with water at any ratio; for example,
It is preferable to use one or more solvents among ethanol, methanol, propylene glycol, etc.).

Although the extraction method is not particularly limited, the extraction is carried out at normal temperature or under heating, and examples of the method include ordinary extraction and Soxhlet extraction. The extraction time is not limited, but is generally preferably 1 hour to 1 week.

The extract may be used as it is,
Various treatments can be performed before use. For example, a concentrated solution obtained by concentrating them under normal pressure or reduced pressure, or a solid obtained by evaporating the solvent in the concentrated solution to dryness, or a solid obtained by crystallizing from the concentrated solution and drying by filtration, or freezing the concentrated solution Dried solids and the like can be mentioned.

The amount (blended amount) of the extract of Bursera lancifolia according to the present invention per dry matter equivalent as an antioxidant is not particularly limited, but is 0.001 to 20% based on the total amount. 0 wt% (hereinafter referred to as wt%), particularly 0.01 to 10 wt% is desirable.

The composition containing the antioxidant of the present invention is characterized by containing the above-mentioned antioxidant, and its use is optional, but it can be used in cosmetics, foods, pharmaceuticals, quasi-drugs, toiletries and the like. Can be widely used.

[0016] The cosmetics to which the present invention is applied are not particularly limited in dosage form, and include, for example, lotions, emulsions, creams,
Examples include foundations, packs, lipsticks, facial cleansers, shampoos, rinses, hair tonics, and the like. These cosmetics include various components generally used in cosmetics, that is, aqueous components, oily components, powder components, alcohols, esters, surfactants, humectants, whitening components, ultraviolet absorbers, thickeners, Components such as coloring agents, fragrances, antioxidants, pH adjusters, chelating agents, preservatives and the like can be added.

The food to which the present invention is applied is not particularly limited, and can be obtained, for example, by adding a required amount of an extract to each food raw material as a general food, and processing and producing the same by a usual production method.

The pharmaceutical and quasi-drugs to which the present invention is applied are not particularly limited in dosage form, for example, tablets, granules,
Examples include internal preparations such as capsules and drenches, external preparations such as ointments, cataplasms, creams and liquid preparations, injections such as sterile solutions and suspensions, and bath preparations. These pharmaceuticals can take the required unit dosage forms with physiologically acceptable vehicles, carriers, excipients, binders, stabilizers, flavoring agents, and the like. For example, compositions for tablets and capsules include binders such as tragacanth, acacia, gelatin, excipients such as microcrystalline cellulose, gelatinized starch, leavening agents such as alginic acid, lubricating agents such as magnesium stearate. Agents, sucrose, lactose, sweeteners such as saccharin, peppermint,
Mix together red oil and flavoring agents like cherry,
It can be prescribed by a usual method. Sterile compositions for injections also dissolve the active substance in vehicles such as water for injection, naturally occurring vegetable oils such as sesame oil, coconut oil, peanut oil, cottonseed oil, or synthetic fat vehicles such as ethyl oleate. Alternatively, it can be formulated by a usual method of suspending. As an external preparation, vaseline, paraffin, oils and fats, lanolin, macrogol and the like are used as bases, and ointments and creams are prepared by a usual method.

The external preparation for skin of the present invention means any dosage form that can be used externally, for example, lotion, emulsion, cream,
Foundations, packs, essences, lipsticks, facial cleansers, gels, aerosols, ointments, cataplasms, pastes, plasters, baths, cleaners, etc., and shampoos, rinses, treatments, hair tonics And the like applied to hair. Further, the external preparation for skin of the present invention can be used for any of medicine, quasi-drug, and cosmetic.

The external preparation for skin of the present invention includes aqueous components, oil components, powder components, waxes, fatty acids, alcohols, esters, surfactants, humectants, which are components used in ordinary skin external preparations. , Whitening ingredients, UV absorbers, thickeners,
A coloring agent, a fragrance, an antioxidant, a pH adjuster, a chelating agent, a preservative, and the like can be appropriately compounded as long as the object of the present invention is achieved.

[0021]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail based on embodiments. Note that the present invention is not limited to these.

[Extraction Example 1] (Production of Bursera lancifolia extract I) Dried Brusera lancifolia
ia) 100 g was extracted with 1 L of methanol at room temperature for 24 hours, and then extracted twice in the same manner. The extracts were combined and concentrated to dryness to obtain 20.3 g of a powdery solid.

[Extraction Example 2] (Production of Bursera lancifolia extract II) Dried Brusera lancifol
ia) 100 g was placed in 1 L of a water / ethanol (1: 1, volume ratio) solvent, extracted with stirring at room temperature for 24 hours, filtered, and the filtrate was concentrated to dryness to obtain a powdery solid.
8 g were obtained.

[Extraction Example 3] (Production of Bursera lancifolia extract III) Dried Brusera lancifolia
ia) 100 g was extracted with 1 L of a mixture of 1,3-butylene glycol and water (1: 1) at room temperature for 10 days, filtered, and the filtrate was concentrated to dryness to obtain 17 g of a powdery solid. .

Test Example 1 The evaluation test method used in the present invention to examine the antioxidant ability of the above extract is as follows. For comparison, rosemary (scientific name: Rosmarinus o.) Already known to have antioxidant power
fficinalis), the same evaluation was performed for a 50% ethanol extract, and 100 ug / ml and 10 ug / ml of the sample were obtained.
, The radical removal rate (%) was determined. (1) Active oxygen elimination test 3.8 mU / ml xanthine oxidase (XOD, 80 mM sodium carbonate buffer (pH 10.2))
Sigma), 0.2 mM EDTA, 100 ug / ml
BSA (Bovine serum albumin, Sigma), 50 uM
It is dissolved so as to become NBT (nitro blue tetrazolium), 0.03 ml of a sample is added to 1.5 ml of this mixture, and pre-incubation is performed at 30 ° C. for 5 minutes. 1.5 ml of 0.2 mM sodium xanthine solution
Is added and left at 30 ° C. for 20 minutes, the reaction is stopped by adding 0.1 ml of a 6 mM cupric chloride solution, and the absorbance (A) is measured at 560 nm. Measure the absorbance (B) of the buffer with the buffer added instead of XOD, the absorbance of the buffer with the buffer added instead of the test sample (C), and the absorbance of the buffer with the buffer added instead of the test sample and XOD (D). Then, the inhibition rate was determined according to the following equation. Active oxygen scavenging rate (%) = (1- (AB) / (C-
D)) × 100

(2) DPPH Radical Scavenging Test Diphenyl-p-picrylhydradil (DP
The inhibitory effect of the extract on the radical chain reaction of lipids was examined using an ethanol solution of PH). 250 mM acetate buffer (pH 5.5) Ethanol 8 in 800 ul
Mix 00 ul and 20 ul of the sample and pre-incubate at 30 ° C for 5 minutes. Add 250 uM DPPH to this solution
400 ul / ethanol solution, add 30 ℃ 30
After standing for 5 minutes, the absorbance at 517 nm was measured,
0 ug / ml BHT (3,5-tert-butyl-4-hydroxytole
n) Add 20 ul, and measure the absorbance when the DPPH radical is completely removed. From this difference, the radical removal rate is determined. The results are shown in Table 1 (Table 1). Bursera lancifolia (Bursera lanc)
ifolia) extract has a DP comparable to rosemary extract
It was found to have a PH radical scavenging action and an active oxygen scavenging action.

[0027]

[Table 1] Table 1 Test DPPH radical removal rate (%) Active oxygen scavenging rate (%) Concentration (ug / ml) 100 10 100 10 例 Extraction example 1 98 100 70 70 Extraction example 2 100 100 73 70 Extraction example 3 100 95 80 75 Rosemary extract 100 33 97 60

[Example 1] (Cream) The B component was mixed with the A component in the following amount, and the mixture was uniformly heated and dissolved to a temperature of 80 ° C. Next, the C component was poured and mixed, and then cooled to 30 ° C. while stirring to obtain a cream. (Composition) Blended ingredients Blended amount (wt%) (A) Squalane 10.0 Olive oil 10.0 Solid paraffin 5.0 Cetanol 4.0 Sorbitan monostearate 2.0 Polyoxyethylene sorbitan monostearate 2.0 (B) Brucella ransifolia extract Extract I (Extraction example) 1) 1.0 (C) Glycerin 5.0 Methyl parapen 0.1 Purified water 100wt% remaining

[Comparative Example 1] (Cream) Except for removing the component (B) Brusera lancifolia extract in Example 1, all were prepared in the same manner as in Example 1 and used in the above-described tests. did.

Example 2 (Two-Phase Lotion) The A component was uniformly mixed and dissolved at room temperature in the following amount, and the B component was slowly added by stirring to obtain a two-phase lotion. (Composition) Ingredients Ingredients Amount (wt%) (A) Olive oil 15.0 Isopropyl myristate 5.0 Polyoxyethylene nonyl phenol ether (2E.O.) 0.5 Glycerin 5.0 Methyl parapen 0.1 Ethanol 7.0 Purified water 65.4 (B) Extract III (extraction) Example 3) 2.0

[Comparative Example 2] (Biphasic lotion) Except for removing the component (B) Bursera lancifolia extract in Example 2, all preparations were carried out in the same manner as in Example 2, and Used for testing.

[Test Example 2] Usefulness evaluation test Eighty healthy women (25 to 40 years old) were divided into four groups of 20 persons each, and the samples of Examples 1 and 2 and Comparative Examples 1 and 2 were taken for 1 day. It was applied twice, and a questionnaire survey was conducted to evaluate the anti-aging effect (prevention of rough skin, gloss / tension of skin) three months after the start of application. The evaluation criteria of the questionnaire were evaluated as "excellent" for valid items, "good" for slightly effective items, "acceptable" for slightly effective items, and "impossible" for invalid items. went. As shown in Table 2 (Table 2), better results were obtained in Examples 1 and 2 than in Comparative Examples 1 and 2.

[0033]

[Table 2] Table 2 ─────────────────────────────── Test Skin roughening prevention effect Skin gloss / tension promoting effect Excellent OK Not possible Excellent OK Not possible ─────────────────────────────── Example 1 15 4 10 19 19 0 0 Comparative Example 1 0 0 0 20 0 1 1 18 Example 2 18 2 0 0 18 2 0 0 Comparative Example 2 0 1 0 1 0 0 0 3 17 ────────────── Note) Number is the number of people

Example 3 (Ointment) An oily ointment was obtained by mixing the components in the following amounts, heating to 80 ° C., and gradually cooling. (Composition) Blended components Blended amount (wt%) (A) Vaseline 96.0 Shortening oil 3.0 (B) Extract I (Extraction Example 1) 1.0

Test Example 3 The oil-based ointment prepared in Example 3 was subjected to an antioxidant test according to the same method as the test method shown in Test Example 1 for evaluating the antioxidant effect. as a result,
The oil-based ointment was excellent in the DPPH radical scavenging action and the active oxygen scavenging action as compared to the oil-based ointment containing all components except that the component (B) was not blended.

[0036]

As described above, compositions such as cosmetics, pharmaceuticals, quasi-drugs, and foods containing the antioxidant of the present invention can be used to prevent disorders caused by active oxygen and lipid peroxide generated in vivo. It has the effect of suppressing. Therefore, it is effective for treating health and cosmetic disorders, and is also useful for stabilizing and improving the preservability of food and drink.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Hiroyuki Haraguchi 11-3 F-term (reference) 11-3 Shimizugaoka, Fukuyama-shi, Hiroshima 4C083 AA111 AA112 AA122 AC012 AC022 AC072 AC102 AC122 AC182 AC352 AC442 AC482 BB47 BB51 CC02 CC05 DD05 DD22 DD28 DD31 EE10 EE12 FF01 FF05 4C088 AB12 AB99 AC03 AC04 AC05 AC06 AC11 AC13 BA08 BA09 BA10 MA63 NA14 ZA33 ZA36 ZA42 ZA45 ZA75 ZA89 ZC21 ZC37

Claims (3)

[Claims] [Claim 1] Bursera lancifolia
An antioxidant comprising an extract of ncifolia). 2. A composition comprising the antioxidant according to claim 1. 3. An external preparation for skin, comprising the antioxidant according to claim 1.