JP2001524320A5 - - Google Patents
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- JP2001524320A5 JP2001524320A5 JP2000522279A JP2000522279A JP2001524320A5 JP 2001524320 A5 JP2001524320 A5 JP 2001524320A5 JP 2000522279 A JP2000522279 A JP 2000522279A JP 2000522279 A JP2000522279 A JP 2000522279A JP 2001524320 A5 JP2001524320 A5 JP 2001524320A5
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- JP
- Japan
- Prior art keywords
- ligand
- seq
- nucleic acid
- acid ligand
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000003446 ligand Substances 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 239000008139 complexing agent Substances 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 血管に対する核酸リガンドであって、配列番号２４２〜２５８に示されるヌクレオチド配列から成る群から選択されるＲＮＡリガンドであることを特徴するリガンド。
【請求項２】 前記リガンドが更に標識を含む、請求項１記載の核酸リガンド。
【請求項３】 前記リガンドが更に錯化剤を含む、請求項１記載の核酸リガンド。
【請求項４】 前記錯化剤が標識を含む、請求項３記載の核酸リガンド。
【請求項５】 前記標識が99m-Tcである、請求項４記載の核酸リガンド。
【請求項１】 血管に対する核酸リガンドであって、配列番号２４２〜２５８に示されるヌクレオチド配列から成る群から選択されるＲＮＡリガンドであることを特徴するリガンド。
【請求項２】 前記リガンドが更に標識を含む、請求項１記載の核酸リガンド。
【請求項３】 前記リガンドが更に錯化剤を含む、請求項１記載の核酸リガンド。
【請求項４】 前記錯化剤が標識を含む、請求項３記載の核酸リガンド。
【請求項５】 前記標識が99m-Tcである、請求項４記載の核酸リガンド。
両方の配列ファミリーからの代表的なクローンは、組織学的オートラジオグラフィーによって、結合に関してクローン12.2と特異的に競合することができた。放射能標識したクローン12.2は、30倍のモル過剰の非標識クローンによる完全な競合を伴う用量依存的な方法において、非標識のクローン12.2（配列番号：242 ）、12.13 （配列番号：246 ）、12.15 （配列番号：248 ）及び12.37 （配列番号：250 ）によって、新鮮な凍結切片から競合脱離（compete off)された。しかしながら、300 倍のモル過剰のクローン12.2のスクランブル配列型の添加によるシグナルの減少は存在しなかった。識別できる配列類似性を有さない2 種の配列ファミリーが、標的結合に関して互いに競合できることがわかった点は興味深い。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/976,413 US6127119A (en) | 1990-06-11 | 1997-11-21 | Nucleic acid ligands of tissue target |
| US08/976,413 | 1997-11-21 | ||
| PCT/US1998/025006 WO1999027138A1 (en) | 1997-11-21 | 1998-11-19 | Nucleic acid ligands of tissue target |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001524320A JP2001524320A (ja) | 2001-12-04 |
| JP2001524320A5 true JP2001524320A5 (ja) | 2009-09-10 |
Family
ID=25524076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000522279A Withdrawn JP2001524320A (ja) | 1997-11-21 | 1998-11-19 | 組織標的に対する核酸リガンド |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6127119A (ja) |
| EP (1) | EP1032708A4 (ja) |
| JP (1) | JP2001524320A (ja) |
| AU (1) | AU750820B2 (ja) |
| CA (1) | CA2311164A1 (ja) |
| WO (1) | WO1999027138A1 (ja) |
Families Citing this family (62)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6232071B1 (en) * | 1990-06-11 | 2001-05-15 | Gilead Sciences, Inc. | Tenascin-C nucleic acid ligands |
| US7005260B1 (en) | 2000-01-28 | 2006-02-28 | Gilead Sciences, Inc. | Tenascin-C nucleic acid ligands |
| DE10019154A1 (de) * | 2000-04-18 | 2001-10-31 | Univ Eberhard Karls | Zellbindende Nukleinsäuremoleküle (Aptamere) |
| US8914114B2 (en) | 2000-05-23 | 2014-12-16 | The Feinstein Institute For Medical Research | Inhibition of inflammatory cytokine production by cholinergic agonists and vagus nerve stimulation |
| US7312325B2 (en) | 2000-09-26 | 2007-12-25 | Duke University | RNA aptamers and methods for identifying the same |
| DK2070939T3 (da) * | 2001-05-25 | 2014-06-23 | Univ Duke | Modulatorer af farmakologiske midler |
| US7206639B2 (en) * | 2002-03-15 | 2007-04-17 | Sarcos Investments Lc | Cochlear drug delivery system and method |
| US7727969B2 (en) | 2003-06-06 | 2010-06-01 | Massachusetts Institute Of Technology | Controlled release nanoparticle having bound oligonucleotide for targeted delivery |
| JP2007525661A (ja) * | 2003-12-12 | 2007-09-06 | セントルイス ユニバーシティー | 大分子その他の分析物の検出用生物センサー |
| US7910297B2 (en) * | 2004-02-27 | 2011-03-22 | Operational Technologies Corporation | Therapeutic nucleic acid-3' -conjugates |
| US8318920B2 (en) * | 2004-02-27 | 2012-11-27 | Operational Technologies Corporation | Therapeutic nucleic acid-3′-conjugates |
| US8389710B2 (en) | 2004-02-27 | 2013-03-05 | Operational Technologies Corporation | Therapeutic nucleic acid-3′-conjugates |
| JP2007530586A (ja) | 2004-03-25 | 2007-11-01 | ザ ファインスタイン インスティテュート フォー メディカル リサーチ | 神経性止血法 |
| US10912712B2 (en) | 2004-03-25 | 2021-02-09 | The Feinstein Institutes For Medical Research | Treatment of bleeding by non-invasive stimulation |
| JP4718541B2 (ja) * | 2004-04-22 | 2011-07-06 | リガド・バイオサイエンシーズ・インコーポレーテツド | 改良された凝固因子調節剤 |
| JP2008525102A (ja) | 2004-12-27 | 2008-07-17 | ノース ショア−ロング アイランド ジューウィッシュ リサーチ インスティテュート | 電気的な迷走神経刺激による炎症性障害の処置 |
| US11207518B2 (en) | 2004-12-27 | 2021-12-28 | The Feinstein Institutes For Medical Research | Treating inflammatory disorders by stimulation of the cholinergic anti-inflammatory pathway |
| US7795009B2 (en) * | 2005-06-15 | 2010-09-14 | Saint Louis University | Three-component biosensors for detecting macromolecules and other analytes |
| US8956857B2 (en) | 2005-06-06 | 2015-02-17 | Mediomics, Llc | Three-component biosensors for detecting macromolecules and other analytes |
| WO2006135527A2 (en) | 2005-06-10 | 2006-12-21 | Saint Louis University | Methods for the selection of aptamers |
| US7811809B2 (en) * | 2005-06-15 | 2010-10-12 | Saint Louis University | Molecular biosensors for use in competition assays |
| US7964356B2 (en) * | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US8975026B2 (en) | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| WO2009029614A1 (en) | 2007-08-27 | 2009-03-05 | The Feinstein Institute For Medical Research | Devices and methods for inhibiting granulocyte activation by neural stimulation |
| WO2009052301A1 (en) * | 2007-10-16 | 2009-04-23 | Regado Biosciences, Inc. | Steady-state subcutaneous administration of aptamers |
| US9662490B2 (en) | 2008-03-31 | 2017-05-30 | The Feinstein Institute For Medical Research | Methods and systems for reducing inflammation by neuromodulation and administration of an anti-inflammatory drug |
| US9211409B2 (en) | 2008-03-31 | 2015-12-15 | The Feinstein Institute For Medical Research | Methods and systems for reducing inflammation by neuromodulation of T-cell activity |
| US8703416B2 (en) | 2008-07-17 | 2014-04-22 | Somalogic, Inc. | Method for purification and identification of sperm cells |
| ES2452484T3 (es) | 2008-11-18 | 2014-04-01 | Setpoint Medical Corporation | Dispositivos para optimizar la colocación de electrodos para la estimulación antiinflamatoria |
| CN102317779A (zh) | 2008-11-21 | 2012-01-11 | 圣路易大学 | 检测目标物多个表位的生物感应器 |
| US9211410B2 (en) | 2009-05-01 | 2015-12-15 | Setpoint Medical Corporation | Extremely low duty-cycle activation of the cholinergic anti-inflammatory pathway to treat chronic inflammation |
| US8996116B2 (en) | 2009-10-30 | 2015-03-31 | Setpoint Medical Corporation | Modulation of the cholinergic anti-inflammatory pathway to treat pain or addiction |
| JP2012528597A (ja) | 2009-06-03 | 2012-11-15 | レガド・バイオサイエンシズ・インク | 血小板グリコプロテインｖｉの核酸調節因子 |
| EP2440284B1 (en) | 2009-06-09 | 2018-09-12 | Setpoint Medical Corporation | Nerve cuff with pocket for leadless stimulator |
| US8236570B2 (en) | 2009-11-03 | 2012-08-07 | Infoscitex | Methods for identifying nucleic acid ligands |
| US8841429B2 (en) | 2009-11-03 | 2014-09-23 | Vivonics, Inc. | Nucleic acid ligands against infectious prions |
| US9833621B2 (en) | 2011-09-23 | 2017-12-05 | Setpoint Medical Corporation | Modulation of sirtuins by vagus nerve stimulation |
| WO2014169145A1 (en) | 2013-04-10 | 2014-10-16 | Setpoint Medical Corporation | Closed-loop vagus nerve stimulation |
| EP2515996B1 (en) | 2009-12-23 | 2019-09-18 | Setpoint Medical Corporation | Neural stimulation devices and systems for treatment of chronic inflammation |
| US20110165037A1 (en) * | 2010-01-07 | 2011-07-07 | Ismagilov Rustem F | Interfaces that eliminate non-specific adsorption, and introduce specific interactions |
| CA2787483C (en) | 2010-02-12 | 2018-03-06 | Saint Louis University | Molecular biosensors capable of signal amplification |
| CN103619405B (zh) | 2011-05-09 | 2015-11-25 | 赛博恩特医疗器械公司 | 用于治疗慢性炎症的胆碱能抗炎通路的单个脉冲激活 |
| US9737480B2 (en) | 2012-02-06 | 2017-08-22 | President And Fellows Of Harvard College | ARRDC1-mediated microvesicles (ARMMs) and uses thereof |
| US9572983B2 (en) | 2012-03-26 | 2017-02-21 | Setpoint Medical Corporation | Devices and methods for modulation of bone erosion |
| CA2938776C (en) | 2014-02-05 | 2023-08-22 | Deakin University | Aptamer construct |
| WO2016040830A1 (en) | 2014-09-12 | 2016-03-17 | Mediomics, Llc | Molecular biosensors with a modular design |
| US11311725B2 (en) | 2014-10-24 | 2022-04-26 | Setpoint Medical Corporation | Systems and methods for stimulating and/or monitoring loci in the brain to treat inflammation and to enhance vagus nerve stimulation |
| US9816080B2 (en) | 2014-10-31 | 2017-11-14 | President And Fellows Of Harvard College | Delivery of CAS9 via ARRDC1-mediated microvesicles (ARMMs) |
| WO2016126807A1 (en) | 2015-02-03 | 2016-08-11 | Setpoint Medical Corporation | Apparatus and method for reminding, prompting, or alerting a patient with an implanted stimulator |
| US11634704B2 (en) | 2015-04-13 | 2023-04-25 | Research Foundation Of The City University Of New York | Ligand-guided-selection method for screening antigen-specific ligands |
| US10253314B2 (en) * | 2015-04-13 | 2019-04-09 | Research Foundation Of The City University Of New York | Ligand-guided-selection method for screening antigen-specific ligands |
| US10596367B2 (en) | 2016-01-13 | 2020-03-24 | Setpoint Medical Corporation | Systems and methods for establishing a nerve block |
| US10695569B2 (en) | 2016-01-20 | 2020-06-30 | Setpoint Medical Corporation | Control of vagal stimulation |
| US10314501B2 (en) | 2016-01-20 | 2019-06-11 | Setpoint Medical Corporation | Implantable microstimulators and inductive charging systems |
| US11471681B2 (en) | 2016-01-20 | 2022-10-18 | Setpoint Medical Corporation | Batteryless implantable microstimulators |
| US10583304B2 (en) | 2016-01-25 | 2020-03-10 | Setpoint Medical Corporation | Implantable neurostimulator having power control and thermal regulation and methods of use |
| EP3518981A4 (en) | 2016-10-03 | 2020-06-10 | President and Fellows of Harvard College | DELIVERING THERAPEUTIC RNAS VIA ARRDC1-MEDIATED MICROVESICLES |
| EP3668402B1 (en) | 2017-08-14 | 2024-07-31 | Setpoint Medical Corporation | Vagus nerve stimulation pre-screening test |
| US11660443B2 (en) | 2018-04-20 | 2023-05-30 | The Feinstein Institutes For Medical Research | Methods and apparatuses for reducing bleeding via electrical trigeminal nerve stimulation |
| US11260229B2 (en) | 2018-09-25 | 2022-03-01 | The Feinstein Institutes For Medical Research | Methods and apparatuses for reducing bleeding via coordinated trigeminal and vagal nerve stimulation |
| AU2020381646A1 (en) * | 2019-11-14 | 2022-04-07 | i-ZUM-X Pty Ltd | Method of detection of fibrin clots |
| CA3178409A1 (en) | 2020-05-21 | 2021-11-25 | Stavros ZANOS | Systems and methods for vagus nerve stimulation |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3590766T (ja) * | 1985-03-30 | 1987-04-23 | ||
| WO1989006694A1 (en) * | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
| US5750342A (en) * | 1990-06-11 | 1998-05-12 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
| US5688935A (en) * | 1990-06-11 | 1997-11-18 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
| US5270163A (en) * | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| CA2104698A1 (en) * | 1991-02-21 | 1992-08-22 | John J. Toole | Aptamers specific for biomolecules and methods of making |
| WO1994006934A1 (en) * | 1992-09-11 | 1994-03-31 | Duke University | Method for deriving epitopes |
| US5614503A (en) * | 1993-11-12 | 1997-03-25 | Aronex Pharmaceuticals, Inc. | Amphipathic nucleic acid transporter |
| DE69633386T2 (de) * | 1995-05-03 | 2005-09-22 | Gilead Sciences, Inc., Foster City | Nukleinsäureliganden für gewebeziele |
-
1997
- 1997-11-21 US US08/976,413 patent/US6127119A/en not_active Expired - Fee Related
-
1998
- 1998-11-19 AU AU16004/99A patent/AU750820B2/en not_active Expired
- 1998-11-19 WO PCT/US1998/025006 patent/WO1999027138A1/en not_active Application Discontinuation
- 1998-11-19 JP JP2000522279A patent/JP2001524320A/ja not_active Withdrawn
- 1998-11-19 EP EP98960403A patent/EP1032708A4/en not_active Withdrawn
- 1998-11-19 CA CA002311164A patent/CA2311164A1/en not_active Abandoned
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