JP2001515204A - 電気流体制御および電気熱制御を有するマイクロ流体システム - Google Patents
電気流体制御および電気熱制御を有するマイクロ流体システムInfo
- Publication number
- JP2001515204A JP2001515204A JP2000508970A JP2000508970A JP2001515204A JP 2001515204 A JP2001515204 A JP 2001515204A JP 2000508970 A JP2000508970 A JP 2000508970A JP 2000508970 A JP2000508970 A JP 2000508970A JP 2001515204 A JP2001515204 A JP 2001515204A
- Authority
- JP
- Japan
- Prior art keywords
- channel
- temperature
- fluid
- current
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012530 fluid Substances 0.000 claims abstract description 470
- 238000000034 method Methods 0.000 claims abstract description 235
- 238000010438 heat treatment Methods 0.000 claims abstract description 131
- 239000000758 substrate Substances 0.000 claims abstract description 88
- 238000006243 chemical reaction Methods 0.000 claims abstract description 46
- 238000012544 monitoring process Methods 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims description 173
- 230000008569 process Effects 0.000 claims description 71
- 150000007523 nucleic acids Chemical class 0.000 claims description 59
- 108020004707 nucleic acids Proteins 0.000 claims description 54
- 102000039446 nucleic acids Human genes 0.000 claims description 54
- 230000003321 amplification Effects 0.000 claims description 39
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 39
- 238000004590 computer program Methods 0.000 claims description 27
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 239000000872 buffer Substances 0.000 claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- 238000004891 communication Methods 0.000 claims description 15
- 230000015654 memory Effects 0.000 claims description 15
- 238000002844 melting Methods 0.000 claims description 14
- 230000008018 melting Effects 0.000 claims description 14
- 230000001351 cycling effect Effects 0.000 claims description 12
- 230000005684 electric field Effects 0.000 claims description 12
- 230000007423 decrease Effects 0.000 claims description 7
- 230000003247 decreasing effect Effects 0.000 claims description 6
- -1 nucleoside triphosphate Chemical class 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 6
- 238000000137 annealing Methods 0.000 claims description 5
- 239000002777 nucleoside Substances 0.000 claims description 2
- 239000001226 triphosphate Substances 0.000 claims description 2
- 235000011178 triphosphate Nutrition 0.000 claims description 2
- 239000012491 analyte Substances 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract description 37
- 238000007834 ligase chain reaction Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 39
- 238000001514 detection method Methods 0.000 description 32
- 230000032258 transport Effects 0.000 description 29
- 238000001816 cooling Methods 0.000 description 27
- 230000006870 function Effects 0.000 description 27
- 238000002474 experimental method Methods 0.000 description 25
- 108020004414 DNA Proteins 0.000 description 21
- 238000012546 transfer Methods 0.000 description 21
- 230000003287 optical effect Effects 0.000 description 19
- 230000004048 modification Effects 0.000 description 17
- 238000012986 modification Methods 0.000 description 17
- 230000001276 controlling effect Effects 0.000 description 15
- 239000013615 primer Substances 0.000 description 14
- 239000000523 sample Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 239000012809 cooling fluid Substances 0.000 description 11
- 238000004925 denaturation Methods 0.000 description 11
- 230000036425 denaturation Effects 0.000 description 11
- 230000008859 change Effects 0.000 description 10
- 239000000700 radioactive tracer Substances 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 230000033001 locomotion Effects 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 238000000926 separation method Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000003556 assay Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000005382 thermal cycling Methods 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 210000004698 lymphocyte Anatomy 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000011065 in-situ storage Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 238000009830 intercalation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000004417 polycarbonate Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000003068 static effect Effects 0.000 description 4
- 230000004544 DNA amplification Effects 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 3
- 239000004926 polymethyl methacrylate Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 235000012239 silicon dioxide Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000005672 electromagnetic field Effects 0.000 description 2
- 238000004049 embossing Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000003505 heat denaturation Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 238000000206 photolithography Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- 238000010791 quenching Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000003631 wet chemical etching Methods 0.000 description 2
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 102100032566 Carbonic anhydrase-related protein 10 Human genes 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 1
- 229910004373 HOAc Inorganic materials 0.000 description 1
- 101000867836 Homo sapiens Carbonic anhydrase-related protein 10 Proteins 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 208000037380 MINOCA Diseases 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 235000013400 Quercus lobata Nutrition 0.000 description 1
- 240000001749 Quercus lobata Species 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000002820 assay format Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000009739 binding Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000004401 flow injection analysis Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hcl hcl Chemical compound Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 238000012188 high-throughput screening assay Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000003698 laser cutting Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- XEBWQGVWTUSTLN-UHFFFAOYSA-M phenylmercury acetate Chemical compound CC(=O)O[Hg]C1=CC=CC=C1 XEBWQGVWTUSTLN-UHFFFAOYSA-M 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 1
- 229920000307 polymer substrate Polymers 0.000 description 1
- 239000012704 polymeric precursor Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229920005591 polysilicon Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000275 quality assurance Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000006903 response to temperature Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
- B01L2200/147—Employing temperature sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1833—Means for temperature control using electrical currents in the sample itself
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5605897P | 1997-09-02 | 1997-09-02 | |
| US60/056,058 | 1997-09-02 | ||
| US08/977,528 US5965410A (en) | 1997-09-02 | 1997-11-25 | Electrical current for controlling fluid parameters in microchannels |
| US08/977,528 | 1997-11-25 | ||
| US8353298P | 1998-04-29 | 1998-04-29 | |
| US60/083,532 | 1998-04-29 | ||
| PCT/US1998/017910 WO1999012016A1 (fr) | 1997-09-02 | 1998-08-27 | Systeme microfluidique a regulation electrofluidique et electrothermique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2001515204A true JP2001515204A (ja) | 2001-09-18 |
Family
ID=27368961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000508970A Pending JP2001515204A (ja) | 1997-09-02 | 1998-08-27 | 電気流体制御および電気熱制御を有するマイクロ流体システム |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1009995A4 (fr) |
| JP (1) | JP2001515204A (fr) |
| AU (1) | AU746098B2 (fr) |
| CA (1) | CA2300203A1 (fr) |
| WO (1) | WO1999012016A1 (fr) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003149252A (ja) * | 2001-11-16 | 2003-05-21 | Starlite Co Ltd | 化学マイクロデバイス |
| JP2005505754A (ja) * | 2001-07-25 | 2005-02-24 | ザ トラスティーズ オブ プリンストン ユニバーシテイ | 高スループットのマクロ分子分析用のナノチャンネル・アレイ並びにその準備および使用 |
| WO2006006679A1 (fr) * | 2004-07-14 | 2006-01-19 | Ebara Corporation | Système de commande de réaction de puce à microcanal, système de réaction micrototal possédant ledit système et système d’analyse micrototale |
| JPWO2004029079A1 (ja) * | 2002-09-25 | 2006-01-26 | 農工大ティー・エル・オー株式会社 | タンパク質複合体の精製方法、アッセイ方法、精製装置およびアッセイ装置 |
| JP2006082079A (ja) * | 2004-09-16 | 2006-03-30 | F Hoffmann La Roche Ag | 流体の温度を調整する方法および装置ならびに微細加工システム |
| JP2006145516A (ja) * | 2004-07-14 | 2006-06-08 | Ebara Corp | マイクロチャンネルチップ反応制御システム、それを含むマイクロトータルリアクションシステムおよびマイクロトータルアナリシスシステム |
| JP2009521682A (ja) * | 2005-12-22 | 2009-06-04 | ハネウェル・インターナショナル・インコーポレーテッド | 携帯用サンプル分析システム |
| JP2009525759A (ja) * | 2006-02-09 | 2009-07-16 | カリパー ライフ サイエンシズ，インコーポレイテッド | マイクロ流体素子内の熱融解曲線を生成する方法および装置 |
| JP2014198337A (ja) * | 2003-08-27 | 2014-10-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 流体種の電子的制御 |
| US20150283546A1 (en) | 2003-04-10 | 2015-10-08 | President And Fellows Of Harvard College | Formation and control of fluidic species |
Families Citing this family (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5885470A (en) | 1997-04-14 | 1999-03-23 | Caliper Technologies Corporation | Controlled fluid transport in microfabricated polymeric substrates |
| EP0907412B1 (fr) | 1996-06-28 | 2008-08-27 | Caliper Life Sciences, Inc. | Systeme de dosage de criblage a fort rendement dans des dispositifs microscopiques de transfert de fluides |
| EP0988529B1 (fr) * | 1997-04-25 | 2013-06-12 | Caliper Life Sciences, Inc. | Dispositifs microfluidiques a geometrie de canaux amelioree |
| US7033474B1 (en) | 1997-04-25 | 2006-04-25 | Caliper Life Sciences, Inc. | Microfluidic devices incorporating improved channel geometries |
| WO1998056956A1 (fr) | 1997-06-09 | 1998-12-17 | Caliper Technologies Corporation | Dispositifs et procedes pour la correction des variations de vitesse dans les systemes microfluidiques |
| US5989402A (en) | 1997-08-29 | 1999-11-23 | Caliper Technologies Corp. | Controller/detector interfaces for microfluidic systems |
| US7497994B2 (en) | 1998-02-24 | 2009-03-03 | Khushroo Gandhi | Microfluidic devices and systems incorporating cover layers |
| US6251343B1 (en) | 1998-02-24 | 2001-06-26 | Caliper Technologies Corp. | Microfluidic devices and systems incorporating cover layers |
| US6756019B1 (en) | 1998-02-24 | 2004-06-29 | Caliper Technologies Corp. | Microfluidic devices and systems incorporating cover layers |
| US6306590B1 (en) | 1998-06-08 | 2001-10-23 | Caliper Technologies Corp. | Microfluidic matrix localization apparatus and methods |
| EP1084391A4 (fr) | 1998-06-08 | 2006-06-14 | Caliper Life Sciences Inc | Dispositifs microfluidiques, systemes et procedes pour realiser des reactions et des separations integrees |
| US6716394B2 (en) | 1998-08-11 | 2004-04-06 | Caliper Technologies Corp. | DNA sequencing using multiple fluorescent labels being distinguishable by their decay times |
| US6447724B1 (en) | 1998-08-11 | 2002-09-10 | Caliper Technologies Corp. | DNA sequencing using multiple fluorescent labels being distinguishable by their decay times |
| US6149787A (en) | 1998-10-14 | 2000-11-21 | Caliper Technologies Corp. | External material accession systems and methods |
| US6416642B1 (en) | 1999-01-21 | 2002-07-09 | Caliper Technologies Corp. | Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection |
| EP2177627B1 (fr) | 1999-02-23 | 2012-05-02 | Caliper Life Sciences, Inc. | Manipulation de microparticules dans des systèmes microfluidiques |
| US6326083B1 (en) | 1999-03-08 | 2001-12-04 | Calipher Technologies Corp. | Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety |
| CN1181337C (zh) | 2000-08-08 | 2004-12-22 | 清华大学 | 微流体系统中实体分子的操纵方法及相关试剂盒 |
| US6500323B1 (en) | 1999-03-26 | 2002-12-31 | Caliper Technologies Corp. | Methods and software for designing microfluidic devices |
| US6303343B1 (en) | 1999-04-06 | 2001-10-16 | Caliper Technologies Corp. | Inefficient fast PCR |
| US6349740B1 (en) * | 1999-04-08 | 2002-02-26 | Abbott Laboratories | Monolithic high performance miniature flow control unit |
| US6458259B1 (en) | 1999-05-11 | 2002-10-01 | Caliper Technologies Corp. | Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow |
| US6592821B1 (en) | 1999-05-17 | 2003-07-15 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
| WO2000070080A1 (fr) | 1999-05-17 | 2000-11-23 | Caliper Technologies Corp. | Focalisation de microparticules dans des systemes microfluidiques |
| WO2000073799A1 (fr) | 1999-06-01 | 2000-12-07 | Caliper Technologies Corp. | Dosages a micro-echelle et dispositifs microfluidiques destines au controle des activites de transporteur, de gradient induit et de liaison |
| US6613580B1 (en) | 1999-07-06 | 2003-09-02 | Caliper Technologies Corp. | Microfluidic systems and methods for determining modulator kinetics |
| ATE419384T1 (de) | 1999-07-28 | 2009-01-15 | Merck Serono Biodevelopment | Durchfluss-mikroreaktor in dem lokale temperaturzyklen auf eine fliessende probe einwirken |
| US6977145B2 (en) | 1999-07-28 | 2005-12-20 | Serono Genetics Institute S.A. | Method for carrying out a biochemical protocol in continuous flow in a microreactor |
| US6858185B1 (en) | 1999-08-25 | 2005-02-22 | Caliper Life Sciences, Inc. | Dilutions in high throughput systems with a single vacuum source |
| JP2003510034A (ja) * | 1999-08-26 | 2003-03-18 | ザ トラスティーズ オブ プリンストン ユニバーシティ | 流体の静電容量の変化を検出するためのマイクロ流体およびナノ流体電子素子並びに使用方法 |
| US6613581B1 (en) | 1999-08-26 | 2003-09-02 | Caliper Technologies Corp. | Microfluidic analytic detection assays, devices, and integrated systems |
| US6537771B1 (en) | 1999-10-08 | 2003-03-25 | Caliper Technologies Corp. | Use of nernstein voltage sensitive dyes in measuring transmembrane voltage |
| US6884395B2 (en) | 2000-05-12 | 2005-04-26 | Gyros Ab | Integrated microfluidic disc |
| US6699713B2 (en) * | 2000-01-04 | 2004-03-02 | The Regents Of The University Of California | Polymerase chain reaction system |
| EP1738829A1 (fr) * | 2000-01-04 | 2007-01-03 | The Regents of the University of California | Systeme d'amplification en chaine par polymerase |
| US6468761B2 (en) | 2000-01-07 | 2002-10-22 | Caliper Technologies, Corp. | Microfluidic in-line labeling method for continuous-flow protease inhibition analysis |
| US7037416B2 (en) | 2000-01-14 | 2006-05-02 | Caliper Life Sciences, Inc. | Method for monitoring flow rate using fluorescent markers |
| US6556923B2 (en) | 2000-01-26 | 2003-04-29 | Caliper Technologies Corp. | Software for high throughput microfluidic systems |
| US20020012971A1 (en) | 2000-03-20 | 2002-01-31 | Mehta Tammy Burd | PCR compatible nucleic acid sieving medium |
| US6733645B1 (en) | 2000-04-18 | 2004-05-11 | Caliper Technologies Corp. | Total analyte quantitation |
| AU6154101A (en) | 2000-05-11 | 2001-11-20 | Caliper Techn Corp | Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers |
| DE60135912D1 (de) | 2000-05-12 | 2008-11-06 | Caliper Life Sciences Inc | Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation |
| WO2002001081A2 (fr) * | 2000-06-23 | 2002-01-03 | Micronics, Inc. | Valve pour structures microfluidiques |
| EP1315570A4 (fr) | 2000-08-03 | 2006-12-13 | Caliper Life Sciences Inc | Procedes et dispositifs pour la distribution de fluides avec une production elevee |
| JP3993372B2 (ja) * | 2000-09-13 | 2007-10-17 | 独立行政法人理化学研究所 | リアクタの製造方法 |
| EP1317569B1 (fr) * | 2000-09-14 | 2009-11-18 | Caliper Life Sciences, Inc. | Dispositifs microfluidiques et procedes permettant la mise en oeuvre de reactions dependant de la temperature |
| CN100495030C (zh) | 2000-09-30 | 2009-06-03 | 清华大学 | 多力操纵装置及其应用 |
| EP1322949A2 (fr) | 2000-10-05 | 2003-07-02 | E.I. Du Pont De Nemours And Company | Dispositif fluidique polymere microfabrique convenant pour la detection dans l'ultraviolet |
| US20090118139A1 (en) | 2000-11-07 | 2009-05-07 | Caliper Life Sciences, Inc. | Microfluidic method and system for enzyme inhibition activity screening |
| JP3628611B2 (ja) * | 2000-11-29 | 2005-03-16 | 独立行政法人科学技術振興機構 | マイクロシステムにおける流れの制御方法 |
| AU2002217067A1 (en) * | 2000-11-29 | 2002-06-11 | Proteologics Inc. | Method and apparatus for decoupling temperature and electric field in electrophoresis separation |
| US6692700B2 (en) | 2001-02-14 | 2004-02-17 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
| JP3941537B2 (ja) * | 2001-02-28 | 2007-07-04 | ソニー株式会社 | 熱輸送装置 |
| US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
| US6852287B2 (en) * | 2001-09-12 | 2005-02-08 | Handylab, Inc. | Microfluidic devices having a reduced number of input and output connections |
| US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
| US7010391B2 (en) | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
| US6575188B2 (en) * | 2001-07-26 | 2003-06-10 | Handylab, Inc. | Methods and systems for fluid control in microfluidic devices |
| US7723123B1 (en) | 2001-06-05 | 2010-05-25 | Caliper Life Sciences, Inc. | Western blot by incorporating an affinity purification zone |
| US9678038B2 (en) | 2001-07-25 | 2017-06-13 | The Trustees Of Princeton University | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
| JP4417116B2 (ja) | 2002-03-05 | 2010-02-17 | カリパー・ライフ・サイエンシズ・インク． | 混合式マイクロ流体システム |
| AU2004245094A1 (en) | 2003-06-06 | 2004-12-16 | Perkinelmer Health Sciences, Inc. | System and method for heating, cooling and heat cycling on microfluidic device |
| US7648835B2 (en) | 2003-06-06 | 2010-01-19 | Micronics, Inc. | System and method for heating, cooling and heat cycling on microfluidic device |
| DE10332289B4 (de) * | 2003-07-16 | 2018-06-14 | Disetronic Licensing Ag | Fluidsystem mit Sicherungseinrichtung |
| EP3718635A1 (fr) | 2003-07-31 | 2020-10-07 | Handylab, Inc. | Traitement d'échantillons contenant des particules |
| US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US8883490B2 (en) | 2006-03-24 | 2014-11-11 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| ES2587007T3 (es) | 2006-03-24 | 2016-10-20 | Handylab, Inc. | Sistema integrado para procesar muestras microfluídicas, y métodos de uso del mismo |
| US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| WO2008061165A2 (fr) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Cartouche microfluidique et son procédé de fabrication |
| EP2604344A3 (fr) | 2007-03-28 | 2014-07-16 | BioNano Genomics, Inc. | Procédés d'analyse macromoléculaire utilisant des réseaux de nanocanaux |
| ES2453107T3 (es) * | 2007-06-11 | 2014-04-04 | Wako Pure Chemical Industries, Ltd. | Procedimiento de amplificación de ácidos nucleicos usando una PCR sobre microchip con detección integrada de CE en tiempo real |
| US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
| US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
| US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
| US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
| ES2648798T3 (es) | 2007-07-13 | 2018-01-08 | Handylab, Inc. | Materiales de captura de polinucleótidos y métodos de utilización de los mismos |
| US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| US8133671B2 (en) | 2007-07-13 | 2012-03-13 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
| JP5279926B2 (ja) * | 2011-03-23 | 2013-09-04 | アークレイ株式会社 | 分析装置、及び分析方法 |
| ES2617599T3 (es) | 2011-04-15 | 2017-06-19 | Becton, Dickinson And Company | Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración |
| USD692162S1 (en) | 2011-09-30 | 2013-10-22 | Becton, Dickinson And Company | Single piece reagent holder |
| EP2761305B1 (fr) | 2011-09-30 | 2017-08-16 | Becton, Dickinson and Company | Bandelette réactive unitisée |
| WO2013067202A1 (fr) | 2011-11-04 | 2013-05-10 | Handylab, Inc. | Dispositif de préparation d'échantillons de polynucléotides |
| US10822644B2 (en) | 2012-02-03 | 2020-11-03 | Becton, Dickinson And Company | External files for distribution of molecular diagnostic tests and determination of compatibility between tests |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5252293A (en) * | 1989-01-17 | 1993-10-12 | Vladimir Drbal | Analytical slide with porous filter membrane |
| US5587128A (en) * | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5637469A (en) * | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
| US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5639423A (en) * | 1992-08-31 | 1997-06-17 | The Regents Of The University Of Calfornia | Microfabricated reactor |
| AU4850793A (en) * | 1992-09-14 | 1994-04-12 | Purdue Research Foundation | Electrophoretically mediated chemical analysis |
| DE69403071D1 (de) * | 1993-03-05 | 1997-06-12 | Jansz Pawliszyn | Verfahren und vorrichtung zur isoelektrofokussierung ohne tragerampholyten |
| US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US5641400A (en) * | 1994-10-19 | 1997-06-24 | Hewlett-Packard Company | Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems |
| US5571410A (en) * | 1994-10-19 | 1996-11-05 | Hewlett Packard Company | Fully integrated miniaturized planar liquid sample handling and analysis device |
| US6132580A (en) * | 1995-09-28 | 2000-10-17 | The Regents Of The University Of California | Miniature reaction chamber and devices incorporating same |
| US5779868A (en) * | 1996-06-28 | 1998-07-14 | Caliper Technologies Corporation | Electropipettor and compensation means for electrophoretic bias |
| US5800690A (en) * | 1996-07-03 | 1998-09-01 | Caliper Technologies Corporation | Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces |
| US5699157A (en) * | 1996-07-16 | 1997-12-16 | Caliper Technologies Corp. | Fourier detection of species migrating in a microchannel |
-
1998
- 1998-08-27 EP EP98944596A patent/EP1009995A4/fr not_active Withdrawn
- 1998-08-27 CA CA002300203A patent/CA2300203A1/fr not_active Abandoned
- 1998-08-27 AU AU92103/98A patent/AU746098B2/en not_active Ceased
- 1998-08-27 WO PCT/US1998/017910 patent/WO1999012016A1/fr active IP Right Grant
- 1998-08-27 JP JP2000508970A patent/JP2001515204A/ja active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013050453A (ja) * | 2001-07-25 | 2013-03-14 | Trustees Of Princeton Univ | 高スループットのマクロ分子分析用のナノチャンネル・アレイ並びにその準備および使用 |
| JP2005505754A (ja) * | 2001-07-25 | 2005-02-24 | ザ トラスティーズ オブ プリンストン ユニバーシテイ | 高スループットのマクロ分子分析用のナノチャンネル・アレイ並びにその準備および使用 |
| JP2009036773A (ja) * | 2001-07-25 | 2009-02-19 | Trustees Of Princeton Univ | 高スループットのマクロ分子分析用のナノチャンネル・アレイ並びにその準備および使用 |
| JP2003149252A (ja) * | 2001-11-16 | 2003-05-21 | Starlite Co Ltd | 化学マイクロデバイス |
| JPWO2004029079A1 (ja) * | 2002-09-25 | 2006-01-26 | 農工大ティー・エル・オー株式会社 | タンパク質複合体の精製方法、アッセイ方法、精製装置およびアッセイ装置 |
| US11141731B2 (en) | 2003-04-10 | 2021-10-12 | President And Fellows Of Harvard College | Formation and control of fluidic species |
| US10293341B2 (en) | 2003-04-10 | 2019-05-21 | President And Fellows Of Harvard College | Formation and control of fluidic species |
| US20150283546A1 (en) | 2003-04-10 | 2015-10-08 | President And Fellows Of Harvard College | Formation and control of fluidic species |
| US11383234B2 (en) | 2003-08-27 | 2022-07-12 | President And Fellows Of Harvard College | Electronic control of fluidic species |
| US10625256B2 (en) | 2003-08-27 | 2020-04-21 | President And Fellows Of Harvard College | Electronic control of fluidic species |
| US9789482B2 (en) | 2003-08-27 | 2017-10-17 | President And Fellows Of Harvard College | Methods of introducing a fluid into droplets |
| US9878325B2 (en) | 2003-08-27 | 2018-01-30 | President And Fellows Of Harvard College | Electronic control of fluidic species |
| JP2014198337A (ja) * | 2003-08-27 | 2014-10-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 流体種の電子的制御 |
| JP2006145516A (ja) * | 2004-07-14 | 2006-06-08 | Ebara Corp | マイクロチャンネルチップ反応制御システム、それを含むマイクロトータルリアクションシステムおよびマイクロトータルアナリシスシステム |
| WO2006006679A1 (fr) * | 2004-07-14 | 2006-01-19 | Ebara Corporation | Système de commande de réaction de puce à microcanal, système de réaction micrototal possédant ledit système et système d’analyse micrototale |
| JP2006082079A (ja) * | 2004-09-16 | 2006-03-30 | F Hoffmann La Roche Ag | 流体の温度を調整する方法および装置ならびに微細加工システム |
| JP2009521682A (ja) * | 2005-12-22 | 2009-06-04 | ハネウェル・インターナショナル・インコーポレーテッド | 携帯用サンプル分析システム |
| JP2016208994A (ja) * | 2006-02-09 | 2016-12-15 | カリパー ライフ サイエンシズ，インコーポレイテッド | マイクロ流体素子内の熱融解曲線を生成する方法および装置 |
| JP2014079252A (ja) * | 2006-02-09 | 2014-05-08 | Caliper Life Sciences Inc | マイクロ流体素子内の熱融解曲線を生成する方法および装置 |
| JP2009525759A (ja) * | 2006-02-09 | 2009-07-16 | カリパー ライフ サイエンシズ，インコーポレイテッド | マイクロ流体素子内の熱融解曲線を生成する方法および装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1009995A1 (fr) | 2000-06-21 |
| WO1999012016A1 (fr) | 1999-03-11 |
| AU746098B2 (en) | 2002-04-18 |
| CA2300203A1 (fr) | 1999-03-11 |
| EP1009995A4 (fr) | 2007-05-02 |
| AU9210398A (en) | 1999-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6174675B1 (en) | Electrical current for controlling fluid parameters in microchannels | |
| AU746098B2 (en) | Microfluidic system with electrofluidic and electrothermal controls | |
| US5965410A (en) | Electrical current for controlling fluid parameters in microchannels | |
| US6750661B2 (en) | Method and apparatus for controllably effecting samples using two signals | |
| US10543466B2 (en) | High resolution temperature profile creation in a digital microfluidic device | |
| US7618811B2 (en) | Thermal cycling device | |
| JP4949600B2 (ja) | 温度介在反応を行うためのマイクロ流体装置及び方法 | |
| US8043849B2 (en) | Thermal cycling device | |
| US20020086439A1 (en) | Integrated devices and systems for performing temperature controlled reactions and analyses | |
| KR101513273B1 (ko) | 회전형 pcr 장치 및 pcr 칩 | |
| US20210178385A1 (en) | Microfluidic devices | |
| JP2010139491A (ja) | 反応液温度測定方法、反応液温度測定装置、反応液温度調整装置及び遺伝子の増幅反応処理を行うための装置 | |
| CN112770841B (zh) | 变温反应器及其加热器和控制电路 | |
| CA2510539A1 (fr) | Systeme microfluidique a regulation electrofluidique et electrothermique | |
| US20020151039A1 (en) | Dna amplification using electrolyte conductance heating and temperature monitoring | |
| Stern et al. | Microfluidic thermocyclers for genetic analysis | |
| Sim | An Integrated Chip-based Device for Droplet-flow Polymerase Chain Reaction | |
| Lagally et al. | Monolithic integrated PCR reactor-CE system for DNA amplification and analysis to the single molecule limit | |
| CN115078313A (zh) | 基于微流控芯片的生物分子分析系统 | |
| Mondal et al. | Miniaturized devices for DNA amplification and fluorescence based detection | |
| Lagally | High-performance integrated DNA analysis microsystems | |
| Kinahan et al. | Thermal Resistance Measurements from a Microchannel Fluorescent Melting Curve Analysis Platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20050422 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20080326 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080625 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080717 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20081001 |