JP2001500370A - P―セレクチンリガンド蛋白 - Google Patents
P―セレクチンリガンド蛋白Info
- Publication number
- JP2001500370A JP2001500370A JP10511682A JP51168298A JP2001500370A JP 2001500370 A JP2001500370 A JP 2001500370A JP 10511682 A JP10511682 A JP 10511682A JP 51168298 A JP51168298 A JP 51168298A JP 2001500370 A JP2001500370 A JP 2001500370A
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- selectin
- seq
- sequence
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.(a)配列番号:2のアミノ酸42からアミノ酸60までを含む第1のア ミノ酸配列、および (b)P−セレクチンリガンド以外の蛋白の配列に由来する第2のアミノ酸配 列 を含む融合蛋白をコードする単離DNA。 2.該ヌクレオチド配列に作動可能に連結された発現制御配列をさらに含む請 求項1のDNA。 3.請求項2のDNAで形質転換された宿主細胞。 4.(a)融合蛋白の発現に適した条件下で請求項3の宿主細胞を培養し;つ いで、 (b)融合蛋白を培地から精製する ことを含む、融合蛋白の製造方法。 5.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸402 までを含むものである請求項1のDNA。 6.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸310 までを含むものである請求項1のDNA。 7.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸88ま でを含むものである請求項1のDNA。 8.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸118 までを含むものである請求項1のDNA。 9.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸189 までを含むものである請求項1のDNA。 10.該第2のアミノ酸配列が該第1のアミノ酸配列のC末端に連結されてい るものである請求項1のDNA。 11.該配列が連結配列により連結されているものである請求項10のDNA 。 12.該第2のアミノ酸配列が該第1のアミノ酸配列のN末端に連結されてい るものである請求項1のDNA。 13.該配列が連結配列により連結されているものである請求項12のDNA 。 14.該第2のアミノ酸配列が抗体、サイトカイン、増殖因子、分化因子、ホ ルモン、酵素、受容体またはそれらのフラグメントならびにリガンドからなる群 より選択される蛋白に由来するものである請求項1のDNA。 15.該第2のアミノ酸配列が抗体の配列に由来するものである請求項14の DNA。 16.該第2のアミノ酸配列が抗体のFc部分に由来するものである請求項1 5のDNA。 17.該第2のアミノ酸配列が抗体由来の配列の変異体である請求項15のD NA。 18.該第2のアミノ酸配列がサイトカインの配列に由来するものである請求 項14のDNA。 19.該第2のアミノ酸配列が増殖因子の配列に由来するものである請求項1 4のDNA。 20.該増殖因子がBMPである請求項19のDNA。 21.配列番号:35のヌクレオチド123からヌクレオチド939までのヌ クレオチド配列を含む請求項7のDNA。 22.配列番号:35のヌクレオチド配列を含む請求項21のDNA。 23.配列番号:37のヌクレオチド123からヌクレオチド807までのヌ クレオチド配列を含む請求項7のDNA。 24.配列番号:37のヌクレオチド配列を含む請求項23のDNA。 25.配列番号:39のヌクレオチド123からヌクレオチド1311までの ヌクレオチド配列を含む請求項7のDNA。 26.配列番号:39のヌクレオチド配列を含む請求項25のDNA。 27.配列番号:41のヌクレオチド123からヌクレオチド792までのヌ クレオチド配列を含む請求項7のDNA。 28.配列番号:41のヌクレオチド配列を含む請求項27のDNA。 29.(a)配列番号:2のアミノ酸42からアミノ酸60までを含む第1の アミノ酸配列、および (b)P−セレクチンリガンド以外の蛋白の配列に由来する第2のアミノ酸配 列 を含む融合蛋白。 30.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸40 2までを含むものである請求項29の融合蛋白。 31.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸31 0までを含むものである請求項29の融合蛋白。 32.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸88 までを含むものである請求項29の融合蛋白。 33.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸11 8までを含むものである請求項29の融合蛋白。 34.該第1のアミノ酸配列が配列番号:2のアミノ酸42からアミノ酸18 9までを含むものである請求項29の融合蛋白。 35.該第2のアミノ酸配列が該第1のアミノ酸配列のC末端に連結されてい るものである請求項29の融合蛋白。 36.該配列が連結配列により連結されているものである請求項35の融合蛋 白。 37.該第2のアミノ酸配列が該第1のアミノ酸配列のN末端に連結されてい るものである請求項29の融合蛋白。 38.該配列が連結配列により連結されているものである請求項37の融合蛋 白。 39.該第2のアミノ酸配列が抗体、サイトカイン、増殖因子、分化因子、ホ ルモン、酵素、受容体またはそれらのフラグメントならびにリガンドからなる群 より選択される蛋白に由来するものである請求項29の融合蛋白。 40.該第2のアミノ酸配列が抗体の配列に由来するものである請求項39の 融合蛋白。 41.該第2のアミノ酸配列が抗体のFc部分に由来するものである請求項4 0の融合蛋白。 42.該第2のアミノ酸配列が抗体由来の配列の変異体である請求項40の融 合蛋白。 43.該第2のアミノ酸配列がサイトカインの配列に由来するものである請求 項39の融合蛋白。 44.該第2のアミノ酸配列が増殖因子の配列に由来するものである請求項3 9の融合蛋白。 45.該増殖因子がBMPである請求項44のDNA。 46.配列番号:36のアミノ酸42からアミノ酸313までのアミノ酸配列 を含む請求項32の融合蛋白。 47.配列番号:36のアミノ酸配列を含む請求項46の融合蛋白。 48.配列番号:38のアミノ酸42からアミノ酸269までのアミノ酸配列 を含む請求項32の融合蛋白。 49.配列番号:38のアミノ酸配列を含む請求項48の融合蛋白。 50.配列番号:40のアミノ酸42からアミノ酸437までのアミノ酸配列 を含む請求項32の融合蛋白。 51.配列番号:40のアミノ酸配列を含む請求項50の融合蛋白。 52.配列番号:42のアミノ酸42からアミノ酸264までを含む請求項3 2の融合蛋白。 53.配列番号:42のアミノ酸配列を含む請求項52の融合蛋白。 54.請求項4の方法により製造される融合蛋白。 55.(a)配列番号:2のアミノ酸42からアミノ酸60までを含む第1の ペプチド、および (b)P−セレクチンリガンド以外の蛋白の配列に由来する第2のペプチド を含む組成物であって、該第1のペプチドおよび該第2のペプチドがペプチド 結合以外の部分により化学結合されているものである組成物。
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US08/713,556 | 1996-08-30 | ||
US08/713,556 US6277975B1 (en) | 1992-10-23 | 1996-08-30 | Fusions of P-selectin ligand protein and polynucleotides encoding same |
PCT/US1997/014159 WO1998008949A1 (en) | 1996-08-30 | 1997-08-29 | P-selectin ligand proteins |
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JP2007271396A Withdrawn JP2008099695A (ja) | 1996-08-30 | 2007-10-18 | P−セレクチンリガンド蛋白 |
JP2008266326A Ceased JP2009060914A (ja) | 1996-08-30 | 2008-10-15 | P−セレクチンリガンド蛋白 |
JP2012014311A Withdrawn JP2012110338A (ja) | 1996-08-30 | 2012-01-26 | P−セレクチンリガンド蛋白 |
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JP2008266326A Ceased JP2009060914A (ja) | 1996-08-30 | 2008-10-15 | P−セレクチンリガンド蛋白 |
JP2012014311A Withdrawn JP2012110338A (ja) | 1996-08-30 | 2012-01-26 | P−セレクチンリガンド蛋白 |
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EP (1) | EP0929670A1 (ja) |
JP (4) | JP4145356B2 (ja) |
AU (1) | AU4149297A (ja) |
CA (1) | CA2263889C (ja) |
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JPH08500722A (ja) | 1992-01-29 | 1996-01-30 | 日立化成工業株式会社 | ポリヌクレオチド固定化担体 |
US5360733A (en) | 1992-10-01 | 1994-11-01 | La Jolla Cancer Research Foundation | Human β1-6 n-acetylglucosaminyl transferase |
CA2497857C (en) | 1992-10-23 | 2009-05-26 | Genetics Institute, Llc | Novel p-selectin ligand protein |
US5843707A (en) | 1992-10-23 | 1998-12-01 | Genetics Institute, Inc. | Nucleic acid encoding a novel P-selectin ligand protein |
US6277975B1 (en) | 1992-10-23 | 2001-08-21 | Genetics Institute, Inc. | Fusions of P-selectin ligand protein and polynucleotides encoding same |
AU681369B2 (en) | 1992-11-16 | 1997-08-28 | Board Of Regents Of The University Of Oklahoma, The | Glycoprotein ligand for P-selectin and methods of use thereof |
WO1995030001A2 (en) * | 1994-04-28 | 1995-11-09 | Genetics Institute, Inc. | Novel p-selectin ligand protein |
US5614615A (en) | 1995-03-21 | 1997-03-25 | The Scripps Research Institute | Sialyl Lewis X mimetics incorporating fucopeptides |
JPH11510543A (ja) | 1995-08-03 | 1999-09-14 | ボード オブ リージェンツ オブ ザ ユニヴァーシティー オブ オクラホマ | ペプチド及びセレクチン介在炎症のo−グリカン阻害剤 |
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JP2012110338A (ja) | 2012-06-14 |
US8232252B2 (en) | 2012-07-31 |
US20080280327A1 (en) | 2008-11-13 |
AU4149297A (en) | 1998-03-19 |
EP0929670A1 (en) | 1999-07-21 |
US20090028883A1 (en) | 2009-01-29 |
US7563760B2 (en) | 2009-07-21 |
JP2008099695A (ja) | 2008-05-01 |
US7927835B2 (en) | 2011-04-19 |
US20090104180A1 (en) | 2009-04-23 |
JP4145356B2 (ja) | 2008-09-03 |
WO1998008949A1 (en) | 1998-03-05 |
US20030018181A1 (en) | 2003-01-23 |
JP2009060914A (ja) | 2009-03-26 |
MXPA99001912A (es) | 2003-07-21 |
CA2263889C (en) | 2007-10-23 |
US6277975B1 (en) | 2001-08-21 |
CA2263889A1 (en) | 1998-03-05 |
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